SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_049766682.1 NCBI__GCF_000016185.1:WP_049766682.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
64% identity, 98% coverage: 11:891/903 of query aligns to 354:1227/1227 of P13009

query
sites
P13009

A

S

L

L

F

F

V

V

N

N

V

V

G

G

E

E

R

R

T

T

N

N

V

V

T

T

G

G

S

S

A

A

R

K

F

F

K

K

K

R

L

L

I

I

L

K

E

E

G

E

N

K

F

Y

E

S

A

E

A

A

L

L

D

D

V

V

A

A

R

R

E

Q

Q

Q

V

V

A

E

N

N

G

G

A

A

Q

Q

V

I

I

I

D

D

V

I

N

N

M

M

D

D

E

E

A

G

M

M

L

L

D

D

S

A

E

E

A

A

M

M

V

V

R

R

F

F

L

L

R

N

L

L

I

I

A

A

S

G

E

E

P

P

D

D

I

I

A

A

R

R

V

V

P

P

V

I

M

M

I

I

D

D

S

S

K

K

W

W

S

D

V

V

I

I

E

E

A

K

G

G

L

L

K

K

N

C

V

I

Q

Q

G

G

K

K

P

G

I

I

V

V

N

N

S

S

I

I

S

S

L

M

K

K

E

E

G

G

E

V

E

D

P

A

F

F

I

I

E

H

H

H

A

A

R

K

K

L

I

L

R

R

Y

Y

G

G

A

A

V

V

M

M

A

A

F

F

D

D

E

E

K

Q

G

G

Q

Q

A

A

D

D

T

T

R

R

D

A

R

R

K

K

V

I

E

E

I

I

C

C

R

R

S

A

Y

Y

D

K

I

I

L

L

V

T

N

E

R

E

V

V

G

G

F

F

P

P

A

P

E

E

D

D

I

I

F

F

D

D

P

P

N

N

V

I

F

F

A

A

V

V

A

A

T

T

G

G

I

I

E

E

H

H

N

N

Y

Y

A

A

V

Q

D

D

F

F

I

I

E

G

A

A

A

C

R

E

L

D

I

I

R

K

R

R

A

E

C

L

P

P

G

H

C

A

H

L

I

I

S

S

G

G

L

V

S

S

N

N

V

V

S

S

F

F

S

S

F

F

R

R

G

G

N

N

N

D

A

P

V

V

R

R

A

E

A

A

M

I

H

H

S

A

V

V

F

F

L

L

Y

Y

H

Y

A

A

I

I

P

R

A

N

G

G

M

M

D

D

M

M

G

G

I

I

V

V

N

N

A

A

G

G

E

Q

L

L

P

A

V

I

Y

Y

D

D

E

D

I

L

P

P

A

A

E

E

L

L

R

R

E

D

R

A

V

V

E

E

D

D

V

V

I

I

L

L

N

N

R

R

P

D

D

D

A

G

T

T

D

E

R

R

L

L

E

E

V

L

A

A

D

E

K

K

Y

Y

K

R

G

G

G

S

A

K

A

T

K

D

E

T

A

A

D

N

L

A

S

Q

-

Q

-

A

-

E

W

W

R

R

Q

S

Q

W

D

E

V

V

R

N

A

K

R

R

L

L

T

E

H

Y

A

S

L

L

V

V

H

K

G

G

I

I

D

T

E

E

Y

F

I

I

D

E

T

Q

D

D

V

T

E

E

A

A

R

R

R

Q

Q

Q

A

A

D

T

R

R

P

P

L

I

S

E

V

V

I

I

E
|
E

G

G

P

P

L

L

M

M

D

D

G

G

M

M

N

N

V

V

G

G

D

D

L

L

F

F

G

G

A

E

G

G

K

K

M

M

F

F

L

L

P

P

Q

Q

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

K

Q

A

A

V

V

A

A

Y

Y

L

L

L

E

P

P

F

F

I

I

E

E

E

A

E

S

K

K

A

E

A

Q

A

G

G

-

S

-

D

K

S

T

A

N

G

G

R

K

V

M

L

V

M

I

A

A

T

T

V

V

K

K

G
|
G

D
|
D

V
|
V

H
|
H

D
|
D

I

I

G

G

K

K

N

N

I

I

V

V

G

G

V

V

L

L

Q

Q

C

C

N

N

F

Y

E

E

V

I

V

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

C

A

A

E

K

K

I

I

L

L

E

R

E

T

A

A

R

K

R

E

H

V

K

N

V

A

D

D

I

L

I

I

G

G

L

L

S
|
S

G

G

L

L

I

I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

V

V

F

N

V

V

A

A

K

K

E

E

M

M

A

E

R

R

E

Q

G

G

F

F

D

T

I

I

P

P

L

L

I

I

G

G

A

A

T

T

S

S

K

K

V

A

H

H

T

T

A

A

V

V

K

K

I

I

D

E

P

Q

H

N

Y

Y

A

S

G

G

P

P

V

T

V

V

H

Y

V

V

L

Q

D

N

A
|
A

S

S

R

R

A

T

V

V

G

G

V

V

A

V

Q

A

N

A

L

L

S

S

A

D

D

T

A

Q

K

R

T

D

G

D

Y

F

A

V

G

A

G

R

I

T

K

R

A

K

D

E

Y

Y

A

E

R

T

M

V

R

R

E

I

A

Q

H

H

E

G

R

R

N

K

Q

K

Y

P

G

R

R

T

A

P

R

P

L

V

S

T

L

L

A

E

D

A

A

A

R

R

A

D

N

N

R

D

F

F

S

A

P

F

D

D

W

W

S

Q

A

A

Y

Y

A

T

P

P

V

-

R

-

P

P

A

V

A

A

P

H

G

R

V

L

T

G

V

V

F

Q

E

E

-

V

D

E

Y

A

D

S

L

I

A

E

E

T

L

L

A

R

T

N

R

Y

I

I

D
|
D

W

W

K

T

F

P

F

F

E

M

A

T

W

W

E

S

L

L

A

A

G

G

S

K

F

Y

P

P

A

R

I

I

L

L

D

E

D

D

E

E

I

V

V

V

G

G

E

V

S

E

A

A

R

Q

G

R

L

L

F

F

K

K

D

D

A

A

Q

N

V

D

M

M

L

L

Q

D

R

K

I

L

I

S

G

A

E

E

K

K

W

T

L

L

T

N

A

P

R

R

G

G

V

V

I

V

G

G

L

L

F

F

P

P

A

A

N

N

S

R

V

V

G

G

D

D

V

I

E

E

I

I

Y

Y

T

R

D

D

E

E

S

T

R

R

T

T

T

H

V

V

L

I

T

N

R

V

L

S

H

H

F

H

L

L

R

R

Q

Q

M

T

A

E

R

K

E

T

P

G

G

-

R

-

E

-

R

F

A

A

H

N

L

Y

C

C

L

L

A

A

D

D

F

F

I

V

A

A

P

P

K

K

Q

L

S

S

G

G

V

K

P

A

D

D

W

Y

I

I

G

G

S

A

F

F

A

A

V

V

T

T

A

G

G

G

H

L

G

E

L

E

E

D

E

A

R

L

A

A

R

D

A

A

F

F

E

E

A

A

A

Q

H

H

D

D

Y

Y

N

N

A

K

I

I

L

M

L

V

K

K

A

A

L

L

G

A

D

D

R

R

L

L

A

A

E

E

A

A

F

F

A

A

E

E

R

Y

M

L

H

H

E

E

R

R

V

V

R

R

R

K

E

V

F

Y

W

W

A

G

Y

Y

A

A

P

P

D

N

E

E

T

N

L

L

S

S

N

N

E

E

L

L

I

I

A

R

E

E

A

N

Y

Y

R

Q

G

G

I

I

R
|
R

P

P

A

A

P

P

G

G

Y

Y

P

P

A

A

C

C

P

P

D

E

H

H

T

T

E

E

K

K

A

A

T

T

L

I

W

W

R

E

L

L

D

E

A

V

E

E

K

K

A

H

A

T

G

G

I

M

S

K

L

L

T

T

E

E

T

S

F

F

A

A

M

M

M

W

P

P

T

G

A

A

A

S

V

V

S

S

G

G

W

W

Y

Y

I

F

A

S

H

H

P

P

E

D

A

S

K

K

Y
|
Y

F
x
Y

G

A

L

V

G

A

K

Q

I

I

E

Q

R

R

D

D

Q

Q

V

V

A

E

D

D

Y

Y

A

A

R

R

K

K

G

G

M

M

E

S

V

V

A

T

A

E

M

V

E

E

R

R

W

W

L

L

A

A

P

P

N

N

L

L

N

G

Y

Y

D

D

P

A

D

D

E694 (= E348) binding methylcob(III)alamin
GDVHD 756:760 (= GDVHD 416:420) binding methylcob(III)alamin
D757 (= D417) mutation to E: Decreases activity by about 70%.; mutation to N: Decreases activity by about 45%.
H759 (= H419) binding axial binding residue; mutation to G: Loss of catalytic activity.
S804 (= S464) binding methylcob(III)alamin
T808 (= T468) binding methylcob(III)alamin
S810 (= S470) mutation to A: Decreases activity by about 40%.
A860 (= A520) binding methylcob(III)alamin
D946 (= D607) binding S-adenosyl-L-methionine
R1134 (= R798) binding S-adenosyl-L-methionine
YY 1189:1190 (≠ YF 853:854) binding S-adenosyl-L-methionine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
247 binding Zn(2+)
310 binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
311 binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.

Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
56% identity, 97% coverage: 13:891/903 of query aligns to 371:1265/1265 of Q99707

query
sites
Q99707

F

F

V

V

N

N

V

I

G

G

E

E

R

R

T

C

N

N

V

V

T

A

G
|
G

S
|
S

A
x
R

R

K

F

F

K

A

K

K

L

L

I

I

L

M

E

A

G

G

N

N

F

Y

E

E

A

E

A

A

L

L

D

C

V

V

A

A

R

K

E

V

Q

Q

V

V

A

E

N

M

G

G

A

A

Q

Q

V

V

I

L

D

D

V

V

N

N

M

M

D

D

E

D

A

G

M

M

L

L

D

D

S

G

E

P

A

S

A

A

M

M

V

T

R

R

F

F

L

C

R

N

L

L

I

I

A

A

S

S

E

E

P

P

D

D

I

I

A

A

R

K

V

V

P

P

V

L

M

C

I

I

D
|
D

S

S

K

N

W

F

S

A

V

V

I

I

E

E

A

A

G

G

L

L

K

K

N

C

V

C

Q

Q

G

G

K

K

P

C

I

I

V

V

N
|
N

S

S

I

I

S

S

L

L

K

K

E

E

G

G

E

E

E

D

P

D

F

F

I

L

E

E

H

K

A

A

R

R

K

K

I

I

R

K

Y

Y

G

G

A

A

V

M

V

V

M

M

A

A

F

F

D

D

E

E

K

E

G

G

Q

Q

A

A

D

T

T

E

R

T

D

D

R

T

K

K

V

I

E

R

I

V

C

C

R

T

R

R

S

A

Y

Y

D

H

I

L

L

L

V

V

N

K

R

K

V

L

G

G

F

F

P

N

A

P

E

N

D

D

I

I

F

F

D
|
D

P

P

N

N

V

I

F

L

A

T

V

I

A

G

T

T

G

G

I

M

E

E

H

H

N

N

N

L

Y

Y

A

A

V

I

D

N

F

F

I

I

E

H

A

A

A

T

R

K

L

V

I

I

R

K

R

E

A

T

C

L

P

P

G

G

C

A

H

R

I

I

S

S

G

G

L

L

S

S

N
|
N

V

L

S

S

F

F

S

S

F

F

R
|
R

G

G

N

M

N

E

A

A

V

I

R
|
R

A

E

A

A

M

M

H

H

S

G

V

V

F

F

L

L

Y

Y

H

H

A

A

I

I

P

K

A

S

G

G

M

M

D

D

M

M

G

G

I

I

V

V

N

N

A

A

G

G

E

N

L

L

P

P

V

V

Y

Y

D

D

E

D

I

I

P

H

A

K

E

E

L

L

R

L

E

Q

R

L

V

C

E

E

D

D

V

L

I

I

L

W

N

N

R

K

R

D

P

P

D

E

A

A

T

T

D

E

R

K

L

L

E

R

V

Y

A

A

D

Q

K

T

Y

Q

K

G

G

T

G

G

A

G

A

K

K

K

K

V

E

I

A

Q

D

T

L

D

S

E

W

W

R

R

Q

N

Q

G

D

P

V

V

R

E

A

E

R

R

L

L

T

E

H

Y

A

A

L

L

V

V

H

K

G

G

I

I

D

E

E

K

Y

H

I

I

D

I

T

E

D

D

V

T

E

E

A

A

R

R

-

L

-

N

-

Q

R

K

Q

K

A

Y

D

P

R

R

P

P

L

L

S

N

V

I

I

I

E

E

G

G

P

P

L

L

M

M

D

N

G

G

M

M

N

K

V

I

V

V

G

G

D

D

L

L

F

F

G

G

A

A

G

G

K

K

M

M

F

F

L

L

P

P

Q

Q

V

V

V

I

K

K

S

S

A

A

R

R

V

V

M

M

K

K

A

A

V

V

A

G

Y

H

L

L

L

I

P

P

F

F

I

M

E

E

E

K

E

E

K

R

A

E

A

T

G

R

G

V

G

L

G

N

G

G

S

T

-

V

-

E

-

D

D

P

S

Y

A

Q

G

G

R

T

V

I

L

V

M

L

A

A

T

T

V

V

K

K

G

G

D

D

V

V

H

H

D

D

I

I

G

G

K

K

N

N

I

I

V

V

G

G

V

V

L

L

Q

G

C

C

N

N

F

F

E

R

V

V

V

I

D

D

L

L

G

G

V

V

M

M

V

T

P

P

C

C

A

D

K

K

I

I

L

L

E

K

E

A

A

A

R

L

R

D

H

H

K

K

V

A

D

D

I

I

G

G

L

L

S

S

G

G

L

L

I

I

T

T

P

P

S

S

L

L

D

D

E

E

M

M

V

I

F

F

V

V

A

A

K

K

E

E

M

M

A

E

R

R

E

L

G

A

F

I

D

R

I

I

P

P

L

L

I

I

G

G

A

A

T

T

S

S

K

K

V

T

H

H

T

T

A

A

V

V

K

K

I

I

D

A

P

P

H

R

Y

Y

A

S

G

A

P

P

V

V

V

I

H

H

V

V

L

L

D

D

A

A

S

S

R

K

A

S

V

V

G

V

V

V

A

C

Q

S

N

Q

L

L

S

D

A

E

D

N

A

L

K

K

T

D

G

E

Y

Y

A

F

G

E

I

I

K

M

A

E

D

E

Y

Y

A

E

R

D

M

I

R

R

E

Q

A
x
D

H

H

E

Y

R

E

N

S

Q

L

Y

K

G

E

R

R

A

R

R

Y

L

L

S

P

L

L

A

S

D

Q

A

A

R

R

A

K

N

S

R

G

F

F

S

Q

P

M

D

D

W

W

-

L

S

S

A

E

Y

P

A

H

P

P

V

V

R

K

P

P

A

T

A

F

P

I

G

G

V

T

T

Q

V

V

F

F

E

E

D
|
D

Y

Y

D

D

L

L

A

Q

E

K

L

L

A

V

T

D

R

Y

I

I

D

D

W

W

K

K

F

P

F

F

E

D

A

V

W

W

E

Q

L

L

A

R

G

G

S

K

-

Y

-

P

-

N

-

R

-

G

F

F

P

P

A

K

I

I

L

F

D

N

D

D

E

K

I

T

V

V

G

G

E

G

S

E

A

A

R

R

G

K

L

V

F

Y

K

D

D

D

A

A

Q

H

V

N

M

M

L

L

Q

N

R

T

I

L

I

I

G

S

E

Q

K

K

W

K

L

L

T

R

A

A

R

R

G

G

V

V

I

V

G

G

L

F

F

W

P

P

A

A

N

Q

S

S

V

I

G

Q

D

D

V

I

E

H

I

L

Y

Y

T

A

D

E

E

A

S

A

R

V

T

P

T

Q

V

A

-

A

-

E

-

P

L

I

T

A

R

T

L

F

H

Y

F

G

L

L

R

R

Q

Q

M

A

A

E

R
x
K

E

D

P

S

G

A

R

S

E

T

R

E

A

P

H

Y

L

Y

C

C

L

L

A

S

D

D

F

F

I

I

A

A

P

P

K

L

Q

H

S

S

G

G

V

I

P

R

D

D

W

Y

I

L

G

G

S

L

F

F

A

A

V

V

T

-

A

A

G

C

H

F

G

G

L

V

E

E

R

L

A

S

R

K

A

A

F

Y

E

E

A

D

H

G

D

D

Y

Y

N

S

A

S

I

I

L

M

L

V

K

K

A

A

L

L

G

G

D

D

R

R

L

L

A

A

E

E

A

A

F

F

A

A

E

E

R

E

M

L

H

H

E

E

R

R

V

V

R

R

E

E

F

L

W

W

A

A

Y

Y

A

C

P

G

D

S

E

E

T

Q

L

L

S

D

N

V

E

A

E

D

L

L

I

R

A

R

E

L

A

R

Y

Y

R

K

G

G

I

I

R

R

P

P

A

A

P

P

G

G

Y

Y

P

P

A

S

C

Q

P

P

D

D

H

H

T

T

E

E

K

K

A

L

T

T

L

M

W

W

R

R

L

L

L

A

D

D

A

I

E

E

K

Q

A

S

A

T

G

G

I

I

S

R

L

L

T

T

E

E

T

S

F

L

A

A

M

M

M

A

P

P

T

A

A

S

A

A

V

V

S

S

G

G

W

L

Y

Y

I

F

A

S

H

N

P

L

E

K

A

S

K

K

Y

Y

F

F

G

A

L

V

G

G

K

K

I

I

E

S

R

K

D

D

Q

Q

V

V

A

E

D

D

Y

Y

A

A

R

L

R

R

K

K

G

N

M

I

E

S

V

V

A

A

A

E

M

V

E

E

R

K

W

W

L

L

A

G

P

P

N

I

L

L

N

G

Y

Y

D

D

P

T

D

D

GSR 382:384 (≠ GSA 24:26) binding (6S)-5,6,7,8-tetrahydrofolate
D449 (= D91) binding (6S)-5,6,7,8-tetrahydrofolate
N470 (= N112) binding (6S)-5,6,7,8-tetrahydrofolate
D537 (= D179) binding (6S)-5,6,7,8-tetrahydrofolate
N579 (= N221) binding (6S)-5,6,7,8-tetrahydrofolate
R585 (= R227) binding (6S)-5,6,7,8-tetrahydrofolate
R591 (= R233) binding (6S)-5,6,7,8-tetrahydrofolate
D919 (≠ A553) to G: in dbSNP:rs1805087
D963 (= D596) mutation to E: Decreases binding to MTRR; when associated with N-1071.
K1071 (≠ R696) mutation to N: Decreases binding to MTRR; when associated with E-963.

Sites not aligning to the query:

61 natural variant: R -> K
255 C → Y: in dbSNP:rs1140598

3bulA E. Coli i690c/g743c meth c-terminal fragment (649-1227) (see paper)
61% identity, 65% coverage: 308:891/903 of query aligns to 4:577/577 of 3bulA

query
sites
3bulA

W

W

R

R

Q

S

Q

W

D

E

V

V

R

N

A

K

R

R

L

L

T

E

H

Y

A

S

L

L

V

V

H

K

G

G

I

I

D

T

E

E

Y

F

I

I

D

E

T

Q

D

D

V

T

E

E

A

A

R

R

R

Q

Q

Q

A

A

D

T

R

R

P

P

L

C

S

E

V

V

I

I

E

E

G

G

P

P

L

L

M

M

D

D

G

G

M

M

N

N

V

V

G

G

D

D

L

L

F

F

G

G

A

E

G

G

K

K

M

M

F

F

L

L

P

P

Q

Q

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

K

Q

A

A

V

V

A

A

Y

Y

L

L

L

E

P

P

F

F

I

I

E

E

E

A

E

S

K

K

A

E

A

Q

A

C

G

-

S

-

D

K

S

T

A

N

G

G

R

K

V

M

L

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V

V

H
|
H

D

D

I

I

G

G

K

K

N

N

I

I

V
|
V

G

G

V

V

L

L

Q

Q

C

C

N

N

F

Y

E

E

V

I

V

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

C

A

A

E

K

K

I

I

L

L

E

R

E

T

A

A

R

K

R

E

H

V

K

N

V

A

D

D

I

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

V

V

F

N

V

V

A

A

K

K

E

E

M

M

A

E

R

R

E

Q

G

G

F

F

D

T

I

I

P

P

L

L

I

I

G
|
G

A

A

T

T

S

S

K

K

V

A

H

H

T

T

A

A

V

V

K

K

I

I

D

E

P

Q

H

N

Y

Y

A

S

G

G

P

P

V

T

V

V

H

Y

V

V

L
x
Q

D
x
N

A
|
A

S

S

R

R

A
x
T

V

V

G

G

V

V

A

V

Q

A

N

A

L

L

S

S

A

D

D

T

A

Q

K

R

T

D

G

D

Y

F

A

V

G

A

G

R

I

T

K

R

A

K

D

E

Y

Y

A

E

R

T

M

V

R

R

E

I

A

Q

H

H

E

G

R

R

N

K

Q

K

Y

P

G

R

R

T

A

P

R

P

L

V

S

T

L

L

A

E

D

A

A

A

R

R

A

D

N

N

R

D

F

F

S

A

P

F

D

D

W

W

S

Q

A

A

Y

Y

A

T

P

P

V

-

R

-

P

P

A

V

A

A

P

H

G

R

V

L

T

G

V

V

F

Q

E

E

-

V

D

E

Y

A

D

S

L

I

A

E

E

T

L

L

A

R

T

N

R

Y

I

I

D

D

W

W

K

T

F

P

F

F

E
x
M

A

T

W

W

E

S

L

L

A

A

G

G

S

K

F

Y

P

P

A

R

I

I

L

L

D

E

D

D

E

E

I

V

V

V

G

G

E

V

S

E

A

A

R

Q

G

R

L

L

F

F

K

K

D

D

A

A

Q

N

V

D

M

M

L

L

Q

D

R

K

I

L

I

S

G

A

E

E

K

K

W

T

L

L

T

N

A

P

R

R

G

G

V

V

I

V

G

G

L

L

F

F

P

P

A

A

N

N

S

R

V

V

G

G

D

D

V

I

E

E

I

I

Y

Y

T

R

D

D

E

E

S

T

R

R

T

T

T

H

V

V

L

I

T

N

R

V

L

S

H

H

F

H

L

L

R

R

Q

Q

M

T

A

E

R

K

E

T

P

G

G

F

R

-

E

-

R

-

A

A

H

N

L

Y

C

C

L

L

A

A

D

D

F

F

I

V

A

A

P

P

K

K

Q

L

S

S

G

G

V

K

P

A

D

D

W

Y

I

I

G

G

S

A

F

F

A

A

V

V

T

T

A

G

G

G

H

L

G

E

L

E

E

D

E

A

R

L

A

A

R

D

A

A

F

F

E

E

A

A

A

Q

H

H

D

D

Y

Y

N

N

A

K

I

I

L

M

L

V

K

K

A

A

L

L

G

A

D
|
D

R

R

L

L

A

A

E

E

A

A

F

F

A

A

E

E

R

Y

M

L

H

H

E

E

R

R

V

V

R

R

R

K

E

V

F

Y

W

W

A

G

Y

Y

A

A

P

P

D

N

E

E

T

N

L

L

S

S

N

N

E

E

L

L

I

I

A

R

E

E

A

N

Y

Y

R

Q

G

G

I

I

R

R

P

P

A
|
A

P
|
P

G
|
G

Y
|
Y

P

P

A

A

C

C

P

P

D

E

H
|
H

T

T

E

E

K
|
K

A

A

T

T

L

I

W

W

R

E

L

L

D

E

A

V

E

E

K

K

A

H

A

T

G

G

I

M

S

K

L

L

T

T

E

E

T

S

F

F

A

A

M
|
M

M

W

P

P

T
x
G

A

A

A

S

V
|
V

S
|
S

G

G

W

W

Y

Y

I

F

A

S

H

H

P

P

E

D

A

S

K

K

Y

Y

F

Y

G

A

L

V

G

A

K

Q

I

I

E

Q

R

R

D

D

Q

Q

V

V

A

E

D

D

Y

Y

A

A

R

R

K

K

G

G

M

M

E

S

V

V

A

T

A

E

M

V

E

E

R

R

W

W

L

L

A

A

P

P

N

N

L

L

N

G

Y

Y

D

D

P

A

D

D

active site: D107 (= D417), H109 (= H419), S160 (= S470)
binding cobalamin: H109 (= H419), V116 (= V426), G152 (= G462), L153 (= L463), S154 (= S464), L156 (= L466), I157 (= I467), T158 (= T468), G183 (= G493), G184 (= G494), Q208 (≠ L518), N209 (≠ D519), A210 (= A520), T213 (≠ A523), M302 (≠ E613), D443 (= D757), A486 (= A800), P487 (= P801), G488 (= G802), Y489 (= Y803), H495 (= H809), K498 (= K812), M521 (= M835), G524 (≠ T838), V527 (= V841), S528 (= S842)

3ivaA Structure of the b12-dependent methionine synthase (meth) c-teminal half with adohcy bound (see paper)
61% identity, 64% coverage: 308:889/903 of query aligns to 4:575/576 of 3ivaA

query
sites
3ivaA

W

W

R

R

Q

S

Q

W

D

E

V

V

R

N

A

K

R

R

L

L

T

E

H

Y

A

S

L

L

V

V

H

K

G

G

I

I

D

T

E

E

Y

F

I

I

D

E

T

Q

D

D

V

T

E

E

A

A

R

R

R

Q

Q

Q

A

A

D

T

R

R

P

P

L

C

S

E

V

V

I

I

E

E

G

G

P

P

L

L

M

M

D

D

G

G

M

M

N

N

V

V

G

G

D

D

L

L

F

F

G

G

A

E

G

G

K

K

M

M

F

F

L

L

P

P

Q

Q

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

K

Q

A

A

V

V

A

A

Y

Y

L

L

L

E

P

P

F

F

I

I

E

E

E

A

E

S

K

K

A

E

A

Q

A

C

G

-

S

-

D

K

S

T

A

N

G

G

R

K

V

M

L

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V

V

H
|
H

D

D

I

I

G
|
G

K

K

N

N

I

I

V
|
V

G

G

V

V

L

L

Q

Q

C

C

N

N

F

Y

E

E

V

I

V

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

C

A

A

E

K

K

I

I

L

L

E

R

E

T

A

A

R

K

R

E

H

V

K

N

V

A

D

D

I

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

V

V

F

N

V

V

A

A

K

K

E

E

M

M

A

E

R

R

E

Q

G

G

F

F

D

T

I

I

P

P

L

L

I

I

G
|
G

A

A

T

T

S

S

K

K

V

A

H

H

T

T

A

A

V

V

K

K

I

I

D

E

P

Q

H

N

Y

Y

A

S

G

G

P

P

V

T

V

V

H

Y

V

V

L
x
Q

D
x
N

A

A

S

S

R

R

A

T

V

V

G

G

V

V

A

V

Q

A

N

A

L

L

S

S

A

D

D

T

A

Q

K

R

T

D

G

D

Y

F

A

V

G

A

G

R

I

T

K

R

A

K

D

E

Y

Y

A

E

R

T

M

V

R

R

E

I

A

Q

H

H

E

G

R

R

N

K

Q

K

Y

P

G

R

R

T

A

P

R

P

L

V

S

T

L

L

A

E

D

A

A

A

R

R

A

D

N

N

R

D

F

F

S

A

P

F

D

D

W

W

S

Q

A

A

Y

Y

A

T

P

P

V

-

R

-

P

P

A

V

A

A

P

H

G

R

V

L

T

G

V

V

F

Q

E

E

-

V

D

E

Y

A

D

S

L

I

A

E

E

T

L

L

A

R

T

N

R

Y

I

I

D

D

W

W

K

T

F

P

F

F

E

M

A
x
T

W

W

E

S

L

L

A

A

G

G

S

K

F

Y

P

P

A

R

I

I

L

L

D

E

D

D

E

E

I

V

V

V

G

G

E

V

S

E

A

A

R

Q

G

R

L

L

F

F

K

K

D

D

A

A

Q

N

V

D

M

M

L

L

Q

D

R

K

I

L

I

S

G

A

E

E

K

K

W

T

L

L

T

N

A

P

R

R

G

G

V

V

I

V

G

G

L

L

F

F

P

P

A

A

N

N

S

R

V

V

G

G

D

D

V

I

E

E

I

I

Y

Y

T

R

D

D

E

E

S

T

R

R

T

T

T

H

V

V

L

I

T

N

R

V

L

S

H

H

F

H

L

L

R

R

Q

Q

M

T

A

E

R

K

E

T

P

G

G

F

R

-

E

-

R

-

A

A

H

N

L

Y

C

C

L

L

A

A

D

D

F

F

I

V

A

A

P

P

K

K

Q

L

S

S

G

G

V

K

P

A

D

D

W

Y

I

I

G

G

S

A

F

F

A

A

V

V

T

T

A

G

G

G

H

L

G

E

L

E

E

D

E

A

R

L

A

A

R

D

A

A

F

F

E

E

A

A

A

Q

H

H

D

D

Y

Y

N

N

A

K

I

I

L

M

L

V

K

K

A

A

L

L

G

A

D
|
D

R

R

L

L

A

A

E
|
E

A

A

F

F

A

A

E

E

R

Y

M

L

H

H

E

E

R

R

V

V

R

R

R

K

E

V

F

Y

W

W

A

G

Y

Y

A

A

P

P

D

N

E

E

T

N

L

L

S

S

N

N

E

E

L

L

I

I

A

R

E

E

A

N

Y

Y

R

Q

G

G

I

I

R
|
R

P
|
P

A
|
A

P

P

G
|
G

Y
|
Y

P

P

A
|
A

C

C

P

P

D

E

H
|
H

T

T

E

E

K

K

A

A

T

T

L

I

W

W

R

E

L

L

D

E

A

V

E

E

K

K

A

H

A

T

G

G

I

M

S

K

L

L

T

T

E

E

T

S

F

F

A
|
A

M
|
M

M

W

P

P

T
x
G

A

A

A

S

V
|
V

S
|
S

G

G

W

W

Y

Y

I

F

A

S

H

H

P

P

E

D

A

S

K

K

Y
|
Y

F

Y

G

A

L

V

G

A

K

Q

I

I

E

Q

R

R

D

D

Q

Q

V

V

A

E

D

D

Y

Y

A

A

R

R

K

K

G

G

M

M

E

S

V

V

A

T

A

E

M

V

E

E

R

R

W

W

L

L

A

A

P

P

N

N

L

L

N

G

Y

Y

D

D

active site: D107 (= D417), H109 (= H419), S160 (= S470)
binding cobalamin: H109 (= H419), G112 (= G422), V116 (= V426), G152 (= G462), L153 (= L463), S154 (= S464), L156 (= L466), I157 (= I467), T158 (= T468), G183 (= G493), G184 (= G494), Q208 (≠ L518), N209 (≠ D519), T303 (≠ A614), D443 (= D757), A486 (= A800), G488 (= G802), Y489 (= Y803), H495 (= H809), A520 (= A834), M521 (= M835), G524 (≠ T838), V527 (= V841), S528 (= S842)
binding s-adenosyl-l-homocysteine: E447 (= E761), R484 (= R798), P485 (= P799), Y489 (= Y803), A491 (= A805), Y539 (= Y853)

3k13C Structure of the pterin-binding domain metr of 5- methyltetrahydrofolate-homocysteine methyltransferase from bacteroides thetaiotaomicron
68% identity, 31% coverage: 13:296/903 of query aligns to 4:286/287 of 3k13C

query
sites
3k13C

F

F

V

V

N

N

V

I

G

G

E
|
E

R

R

T

C

N

N

V

V

T

A

G
|
G

S

S

A
x
R

R

K

F

F

K

L

K

R

L

L

I

V

L

N

E

E

G

K

N

K

F

Y

E

D

A

E

A

A

L

L

D

S

V

I

A

A

R

R

E

Q

Q

Q

V

V

A

E

N

D

G

G

A

A

Q

L

V

V

I

I

D

D

V

V

N

N

M

M

D

D

E

D

A

G

M

L

L

L

D

D

S

A

E

R

A

T

A

E

M

M

V

T

R

T

F

F

L

L

R

N

L

L

I

I

A

M

S

S

E

E

P

P

D

E

I

I

A

A

R

R

V

V

P

P

V

V

M

M

I

I

D

D

S

S

K

K

W

W

S

E

V

V

I

I

E

E

A

A

G

G

L

L

K

K

N

C

V

L

Q

Q

G

G

K

K

P

S

I

I

V

V

N
|
N

S

S

I

I

S

S

L

L

K

K

E

E

G

G

E

E

P

V

F

F

I

L

E

E

H

H

A

A

R

R

K

I

I

I

R

K

R

Q

Y

Y

G

G

A

A

V

T

V

V

M

M

A

A

F

F

D

D

E

E

K

K

G

G

Q

Q

A

A

D

D

T

T

R

A

D

A

R

R

K

K

V

I

E

E

I

V

C

C

R

E

R

R

S

A

Y

Y

D

R

I

L

L

L

V

V

N

D

R

K

V

V

G

G

F

F

P

N

A

P

E

H

D

D

I

I

F

F

D
|
D

P

P

N

N

V

V

F

L

A

A

V

V

A

A

T

T

G

G

I

I

E

E

H

H

N

N

Y

Y

A

A

V

V

D

D

F

F

I

I

E

E

A

A

A

T

R

G

L

W

I

I

R

R

R

K

A

N

C

L

P

P

G

G

C

A

H

H

I

V

S

S

G

G

G
|
G

L

V

S
|
S

N
|
N

V

L

S

S

F

F

S

S

F

F

R
|
R

G

G

N

N

A

Y

V

I

R
|
R

A

E

A

A

M

M

H

H

S

A

V

V

F

F

L

L

Y

Y

H

H

A

A

I

I

P

Q

A

Q

G

G

M

M

D

D

M

M

G

G

I
|
I

V

V

N

N

A

P

G

G

E

S

L

V

P

-

V

L

Y

Y

D

S

E

D

I

I

P

P

A

A

E

D

L

T

R

L

E

E

R

K

V

I

E

E

D

D

V

V

I

V

L

L

N

N

R

R

P

P

D

D

A

A

T

A

D

E

R

R

L

L

L

I

E

E

V

L

A

A

D

E

K

A

Y

L

K

K

binding n-[4-({[(6s)-2-amino-4-hydroxy-5-methyl-5,6,7,8-tetrahydropteridin-6-yl]methyl}amino)benzoyl]-l-glutamic acid: E9 (= E18), G15 (= G24), R17 (≠ A26), N103 (= N112), D170 (= D179), G209 (= G218), S211 (= S220), N212 (= N221), R218 (= R227), R224 (= R233), I244 (= I253)

1mskA Methionine synthase (activation domain) (see paper)
59% identity, 36% coverage: 564:891/903 of query aligns to 4:327/327 of 1mskA

query
sites
1mskA

L

V

S

T

L

L

A

E

D

A

A

A

R

R

A

D

N

N

R

D

F

F

S

A

P

F

D

D

W

W

S

Q

A

A

Y

Y

A

T

P

P

V

-

R

-

P

P

A

V

A

A

P

H

G

R

V

L

T

G

V

V

F

Q

E

E

-

V

D

E

Y

A

D

S

L

I

A

E

E

T

L

L

A

R

T

N

R

Y

I

I

D
|
D

W

W

K

T

F

P

F

F

E

M

A

T

W

W

E

S

L

L

A

A

G

G

S

K

F

Y

P

P

A

R

I

I

L

L

D

E

D

D

E

E

I

V

V

V

G

G

E

V

S

E

A

A

R

Q

G

R

L

L

F

F

K

K

D

D

A

A

Q

N

V

D

M

M

L

L

Q

D

R

K

I

L

I

S

G

A

E

E

K

K

W

T

L

L

T

N

A

P

R

R

G

G

V

V

I

V

G

G

L

L

F

F

P

P

A

A

N

N

S

R

V

V

G

G

D

D

V

I

E

E

I

I

Y

Y

T

R

D

D

E

E

S

T

R

R

T

T

T

H

V

V

L

I

T

N

R

V

L

S

H

H

F

H

L

L

R

R

Q

Q

M

T

A

E

R

K

E

-

P

-

G

-

R

T

E

G

R

F

A

A

H

N

L

Y

C

C

L

L

A

A

D

D

F

F

I

V

A

A

P

P

K

K

Q

L

S

S

G

G

V

K

P

A

D

D

W

Y

I

I

G

G

S

A

F

F

A

A

V

V

T

T

A

G

G

G

H

L

G

E

L

E

E

D

E

A

R

L

A

A

R

D

A

A

F

F

E

E

A

A

A

Q

H

H

D

D

Y

Y

N

N

A

K

I

I

L

M

L

V

K

K

A

A

L

L

G

A

D

D

R

R

L

L

A

A

E

E

A

A

F

F

A

A

E

E

R

Y

M

L

H

H

E

E

R

R

V

V

R

R

R

K

E

V

F

Y

W

W

A

G

Y

Y

A

A

P

P

D

N

E

E

T

N

L

L

S

S

N

N

E

E

L

L

I

I

A

R

E

E

A

N

Y

Y

R

Q

G

G

I

I

R
|
R

P
|
P

A

A

P

P

G

G

Y
|
Y

P

P

A
|
A

C

C

P

P

D

E

H

H

T

T

E

E

K

K

A

A

T

T

L

I

W

W

R

E

L

L

D

E

A

V

E

E

K

K

A

H

A

T

G

G

I

M

S

K

L

L

T

T

E

E

T

S

F

F

A

A

M

M

M

W

P

P

T

G

A

A

A

S

V

V

S

S

G

G

W

W

Y

Y

I

F

A

S

H

H

P

P

E

D

A

S

K

K

Y
|
Y

F

Y

G

A

L

V

G

A

K

Q

I

I

E

Q

R

R

D

D

Q

Q

V

V

A

E

D

D

Y

Y

A

A

R

R

K

K

G

G

M

M

E

S

V

V

A

T

A

E

M

V

E

E

R

R

W

W

L

L

A

A

P

P

N

N

L

L

N

G

Y

Y

D

D

P

A

D

D

binding s-adenosylmethionine: D46 (= D607), R234 (= R798), P235 (= P799), Y239 (= Y803), A241 (= A805), Y289 (= Y853)

6bdyA Crystal structure of the meth reactivation domain bound to sinefungin (see paper)
59% identity, 36% coverage: 564:889/903 of query aligns to 4:325/326 of 6bdyA

query
sites
6bdyA

L

V

S

T

L

L

A

E

D

A

A

A

R

R

A

D

N

N

R

D

F

F

S

A

P

F

D

D

W

W

S

Q

A

A

Y

Y

A

T

P

P

V

-

R

-

P

P

A

V

A

A

P

H

G

R

V

L

T

G

V

V

F

Q

E

E

-

V

D

E

Y

A

D

S

L

I

A

E

E

T

L

L

A

R

T

N

R

Y

I

I

D
|
D

W

W

K

T

F

P

F

F

E

M

A

T

W

W

E

S

L

L

A

A

G

G

S

K

F

Y

P

P

A

R

I

I

L

L

D

E

D

D

E

E

I

V

V

V

G

G

E

V

S

E

A

A

R

Q

G

R

L

L

F

F

K

K

D

D

A

A

Q

N

V

D

M

M

L

L

Q

D

R

K

I

L

I

S

G

A

E

E

K

K

W

T

L

L

T

N

A

P

R

R

G

G

V

V

I

V

G

G

L

L

F

F

P

P

A

A

N

N

S

R

V

V

G

G

D

D

V

I

E

E

I

I

Y

Y

T

R

D

D

E

E

S

T

R

R

T

T

T

H

V

V

L

I

T

N

R

V

L

S

H

H

F

H

L

L

R

R

Q

Q

M

T

A

E

R

K

E

-

P

-

G

-

R

T

E

G

R

F

A

A

H

N

L

Y

C

C

L

L

A

A

D

D

F

F

I

V

A

A

P

P

K

K

Q

L

S

S

G

G

V

K

P

A

D

D

W

Y

I

I

G

G

S

A

F

F

A

A

V

V

T

T

A

G

G

G

H

L

G

E

L

E

E

D

E

A

R

L

A

A

R

D

A

A

F

F

E

E

A

A

A

Q

H

H

D

D

Y

Y

N

N

A

K

I

I

L

M

L

V

K

K

A

A

L

L

G

A

D

D

R

R

L

L

A

A

E

E

A

A

F

F

A

A

E

E

R

Y

M

L

H

H

E

E

R

R

V

V

R

R

R

K

E

V

F

Y

W

W

A

G

Y

Y

A

A

P

P

D

N

E

E

T

N

L

L

S

S

N

N

E

E

L

L

I

I

A

R

E

E

A

N

Y

Y

R

Q

G

G

I

I

R
|
R

P
|
P

A
|
A

P

P

G

G

Y
|
Y

P
|
P

A
|
A

C

C

P

P

D

E

H

H

T

T

E

E

K

K

A

A

T

T

L

I

W

W

R

E

L

L

D

E

A

V

E

E

K

K

A

H

A

T

G

G

I

M

S

K

L

L

T

T

E

E

T

S

F

F

A

A

M

M

M

W

P

P

T

G

A

A

A

S

V

V

S

S

G

G

W

W

Y

Y

I

F

A

S

H

H

P

P

E

D

A

S

K

K

Y
|
Y

F

Y

G

A

L

V

G

A

K

Q

I

I

E

Q

R

R

D

D

Q

Q

V

V

A

E

D

D

Y

Y

A

A

R

R

K

K

G

G

M

M

E

S

V

V

A

T

A

E

M

V

E

E

R

R

W

W

L

L

A

A

P

P

N

N

L

L

N

G

Y

Y

D

D

binding sinefungin: D46 (= D607), R234 (= R798), P235 (= P799), A236 (= A800), Y239 (= Y803), P240 (= P804), A241 (= A805), Y289 (= Y853)

4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
64% identity, 31% coverage: 13:292/903 of query aligns to 331:610/611 of 4cczA

query
sites
4cczA

F

F

V

V

N

N

V

I

G

G

E
|
E

R

R

T

C

N

N

V

V

T

A

G
|
G

S

S

A
x
R

R

K

F

F

K

A

K

K

L

L

I

I

L

M

E

A

G

G

N

N

F

Y

E

E

A

E

A

A

L

L

D

C

V

V

A

A

R

K

E

V

Q

Q

V

V

A

E

N

M

G

G

A

A

Q

Q

V

V

I

L

D

D

V

V

N

N

M

M

D

D

E

D

A

G

M

M

L

L

D

D

S

G

E

P

A

S

A

A

M

M

V

T

R

R

F

F

L

C

R

N

L

L

I

I

A

A

S

S

E

E

P

P

D

D

I

I

A

A

R

K

V

V

P

P

V

L

M

C

I

I

D

D

S

S

K

N

W

F

S

A

V

V

I

I

E

E

A

A

G

G

L

L

K

K

N

C

V

C

Q

Q

G

G

K

K

P

C

I

I

V

V

N
|
N

S

S

I

I

S

S

L

L

K

K

E

E

G

G

E

E

E

D

P

D

F

F

I

L

E

E

H

K

A

A

R

R

K

K

I

I

R

K

Y

Y

G

G

A

A

V

M

V

V

M
|
M

A

A

F

F

D

D

E

E

K

E

G

G

Q

Q

A

A

D

T

T

E

R

T

D

D

R

T

K

K

V

I

E

R

I

V

C

C

R

T

R

R

S

A

Y

Y

D

H

I

L

L

L

V

V

N

K

R

K

V

L

G

G

F

F

P

N

A

P

E

N

D

D

I

I

F

F

D
|
D

P

P

N

N

V

I

F

L

A

T

V

I

A

G

T

T

G

G

I

M

E

E

H

H

N

N

N

L

Y

Y

A

A

V

I

D

N

F

F

I

I

E

H

A

A

A

T

R

K

L

V

I

I

R

K

R

E

A

T

C

L

P

P

G

G

C

A

H

R

I

I

S

S

G

G

G
|
G

L

L

S
|
S

N
|
N

V

L

S

S

F
|
F

S

S

F

F

R
|
R

G

G

N

M

N

E

A

A

V

I

R
|
R

A

E

A

A

M

M

H

H

S

G

V

V

F

F

L

L

Y

Y

H

H

A

A

I

I

P

K

A

S

G

G

M

M

D

D

M

M

G

G

I

I

V

V

N

N

A

A

G

G

E

N

L

L

P

P

V

V

Y

Y

D

D

E

D

I

I

P

H

A

K

E

E

L

L

R

L

E

Q

R

L

V

C

E

E

D

D

V

L

I

I

L

W

N

N

R

K

R

D

P

P

D

E

A

A

T

T

D

E

R

K

L

L

E

R

V

Y

A

A

binding (6s)-5,6,7,8-tetrahydrofolate: E336 (= E18), G342 (= G24), R344 (≠ A26), N430 (= N112), M458 (= M140), D497 (= D179), G536 (= G218), S538 (= S220), N539 (= N221), F542 (= F224), R545 (= R227), R551 (= R233)

1bmtA How a protein binds b12: a 3.O angstrom x-ray structure of the b12- binding domains of methionine synthase (see paper)
65% identity, 28% coverage: 308:556/903 of query aligns to 4:246/246 of 1bmtA

query
sites
1bmtA

W

W

R

R

Q

S

Q

W

D

E

V

V

R

N

A

K

R

R

L

L

T

E

H

Y

A

S

L

L

V

V

H

K

G

G

I

I

D

T

E

E

Y

F

I

I

D

E

T

Q

D

D

V

T

E

E

A

A

R

R

R

Q

Q

Q

A

A

D

T

R

R

P

P

L

I

S

E

V

V

I

I

E
|
E

G

G

P

P

L

L

M
|
M

D

D

G

G

M
|
M

N

N

V

V

G
|
G

D

D

L

L

F

F

G

G

A

E

G

G

K

K

M

M

F

F

L
|
L

P

P

Q

Q

V
|
V

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

K

Q

A

A

V

V

A

A

Y

Y

L

L

L

E

P

P

F

F

I

I

E

E

E

A

E

S

K

K

A

E

A

Q

A

G

G

-

S

-

D

K

S

T

A

N

G

G

R

K

V

M

L

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V
|
V

H
|
H

D
|
D

I
|
I

G

G

K

K

N

N

I
|
I

V

V

G

G

V

V

L

L

Q

Q

C

C

N

N

F

Y

E

E

V

I

V

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

C

A

A

E

K

K

I

I

L

L

E

R

E

T

A

A

R

K

R

E

H

V

K

N

V

A

D

D

I

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

V

V

F

N

V

V

A

A

K

K

E

E

M

M

A

E

R

R

E

Q

G

G

F

F

D

T

I

I

P

P

L

L

I

I

G
|
G

A
|
A

T

T

S

S

K

K

V

A

H

H

T

T

A

A

V

V

K

K

I

I

D

E

P

Q

H

N

Y

Y

A

S

G

G

P

P

V

T

V

V

H

Y

V
|
V

L

Q

D
x
N

A
|
A

S

S

R

R

A

T

V

V

G

G

V

V

A

V

Q

A

N

A

L

L

S

S

A

D

D

T

A

Q

K

R

T

D

G

D

Y

F

A

V

G

A

G

R

I

T

K

R

A

K

D

E

Y

Y

A

E

R

T

M

V

R

R

E

I

A

Q

H

H

E

G

R

R

active site: D107 (= D417), H109 (= H419), S160 (= S470)
binding co-methylcobalamin: E44 (= E348), M48 (= M352), M51 (= M355), G55 (= G359), L65 (= L369), V68 (= V372), D107 (= D417), V108 (= V418), H109 (= H419), D110 (= D420), I111 (= I421), I115 (= I425), G152 (= G462), L153 (= L463), S154 (= S464), L156 (= L466), I157 (= I467), T158 (= T468), G183 (= G493), G184 (= G494), A185 (= A495), V207 (= V517), N209 (≠ D519), A210 (= A520)

8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
37% identity, 55% coverage: 10:502/903 of query aligns to 330:806/841 of 8g3hA

query
sites
8g3hA

T
|
T

A
x
S

L

L

F

L

V

L

N

-

V

V

G

G

E

E

R

R

T

L

N

N

V

A

T

T

G

G

S

S

A

K

R

R

F

F

K

R

K

E

L

L

I

L

L

F

E

A

G

G

N

D

F

L

E

E

A

G

A

I

L

L

D

A

V

L

A

A