SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_049774007.1 NCBI__GCF_000015565.1:WP_049774007.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
34% identity, 85% coverage: 11:241/273 of query aligns to 16:246/265 of P07821

query
sites
P07821

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K50 (= K45) mutation to Q: Lack of activity.
D172 (= D169) mutation to E: Lack of activity.
E173 (= E170) mutation to A: Lack of activity.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
33% identity, 84% coverage: 16:245/273 of query aligns to 12:240/241 of 4u00A

query
sites
4u00A

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binding adenosine-5'-diphosphate: F12 (≠ I16), V17 (≠ I21), S37 (≠ N41), G38 (= G42), S39 (≠ A43), G40 (= G44), K41 (= K45), S42 (= S46), T43 (= T47)

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
33% identity, 79% coverage: 7:222/273 of query aligns to 4:217/223 of 2pclA

query
sites
2pclA

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binding magnesium ion: E165 (= E170), P166 (= P171), T167 (≠ L172), T196 (≠ L201)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
29% identity, 79% coverage: 9:225/273 of query aligns to 4:218/240 of 4ymuJ

query
sites
4ymuJ

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binding adenosine-5'-triphosphate: F11 (≠ I16), V16 (≠ I21), S36 (≠ N41), G37 (= G42), S38 (≠ A43), G39 (= G44), K40 (= K45), S41 (= S46), T42 (= T47), E162 (= E170), H194 (= H202)
binding magnesium ion: S41 (= S46), E162 (= E170)

6panA Structure of a bacterial atm1-family abc exporter with atp bound (see paper)
34% identity, 76% coverage: 19:225/273 of query aligns to 344:547/572 of 6panA

query
sites
6panA

R
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binding adenosine-5'-triphosphate: R344 (= R19), I346 (= I21), S366 (≠ N41), G367 (= G42), K370 (= K45), S371 (= S46), T372 (= T47), K467 (≠ Q144), S469 (= S146), G470 (= G147), E472 (= E149), H524 (= H202)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 340

4mrvA Structure of a bacterial atm1-family abc transporter (see paper)
34% identity, 76% coverage: 19:225/273 of query aligns to 367:570/600 of 4mrvA

query
sites
4mrvA

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K

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A

M

Y

L

-

G

G

R

R

L

D

P

G

H

A

Q

S

A

R

W

A

L

E

A

V

P

D

P

A

G

A

P

A

A

K

D

G

H

A

A

A

A

I

V

A

E

D

Q

F

A

I

L

A

R

R

A

L

T

P

Q

Q

A

G

W

Y

D

D

W

T

R

E

-

V

-

G

Q

E

R

R

P

G

L

L

S

-

Q

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

L

A

L

I

A

A

R

R

A

T

L

L

A

V

V

K

Q

N

A

P

P

P

V

I

L

L

L

F

D

D

E

E

P

A

L

T

A

S

N

A

L

L

D

D

P

T

P

R

H

T

Q

E

T

Q

D

D

W

I

L

L

H

S

T

T

M

M

R

R

T

A

L

-

V

V

A

A

A

S

G

H

T

R

T

T

V

T

V

I

S

S

V

I

L

A

H

H

E

R

V

L

S

S

L

T

A

I

L

A

Q

D

A

S

D

D

D

T

M

I

V

L

L

V

M

L

A

D

A

Q

G

G

R

R

V

L

L

A

H

E

Q

Q

G

G

binding phosphate ion: S389 (≠ N41), G392 (= G44), S394 (= S46)

Sites not aligning to the query:

binding mercury bis(L-gamma-glutamyl-3-sulfido-L-alanylglycine): 149, 261, 262, 265, 309, 310, 311, 312, 313
binding phosphate ion: 313, 316

4mrsA Structure of a bacterial atm1-family abc transporter (see paper)
34% identity, 76% coverage: 19:225/273 of query aligns to 367:570/600 of 4mrsA

query
sites
4mrsA

R

R

Q

E

I

I

L

L

H

H

G

G

I

L

D

S

L

F

Q

E

L

V

P

A

A

A

G

G

R

S

W

R

T

V

S

A

I

I

V

V

G

G

P

P

N
x
S

G

G

A

A

G
|
G

K

K

S
|
S

T

T

L

I

L

-

K

-

A

-

L

-

A

A

G

R

L

L

P

F

R

R

A

F

A

Y

V

D

-

P

-

W

Q

E

G

G

R

R

V

I

Q

L

L

I

L

D

G

G

R

Q

A

D

L

I

A

A

Q

H

I

V

P

T

A

Q

R

T

E

S

R

L

A

R

R

A

Q

A

L

L

A

G

W

I

L

V

G

P

Q

Q

G

D

E

S

G

V

V

L

T

F

D

N

E

D

L

T

T

I

S

G

Y

Y

D

N

I

I

A

A

M

Y

L

-

G

G

R

R

L

D

P

G

H

A

Q

S

A

R

W

A

L

E

A

V

P

D

P

A

G

A

P

A

A

K

D

G

H

A

A

A

A

I

V

A

E

D

Q

F

A

I

L

A

R

R

A

L

T

P

Q

Q

A

G

W

Y

D

D

W

T

R

E

-

V

-

G

Q

E

R

R

P

G

L

L

S

-

Q

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

L

A

L

I

A

A

R

R

A

T

L

L

A

V

V

K

Q

N

A

P

P

P

V

I

L

L

L

F

D

D

E

E

P

A

L

T

A

S

N

A

L

L

D

D

P

T

P

R

H

T

Q

E

T

Q

D

D

W

I

L

L

H

S

T

T

M

M

R

R

T

A

L

-

V

V

A

A

A

S

G

H

T

R

T

T

V

T

V

I

S

S

V

I

L

A

H

H

E

R

V

L

S

S

L

T

A

I

L

A

Q

D

A

S

D

D

D

T

M

I

V

L

L

V

M

L

A

D

A

Q

G

G

R

R

V

L

L

A

H

E

Q

Q

G

G

binding phosphate ion: S389 (≠ N41), G392 (= G44), S394 (= S46)

Sites not aligning to the query:

binding oxidized glutathione disulfide: 142, 149, 199, 203, 261, 262, 265, 309, 310, 312, 313

4mrpA Structure of a bacterial atm1-family abc transporter (see paper)
34% identity, 76% coverage: 19:225/273 of query aligns to 367:570/600 of 4mrpA

query
sites
4mrpA

R

R

Q

E

I

I

L

L

H

H

G

G

I

L

D

S

L

F

Q

E

L

V

P

A

A

A

G

G

R

S

W

R

T

V

S

A

I

I

V

V

G

G

P

P

N
x
S

G
|
G

A

A

G
|
G

K

K

S
|
S

T

T

L

I

L

-

K

-

A

-

L

-

A

A

G

R

L

L

P

F

R

R

A

F

A

Y

V

D

-

P

-

W

Q

E

G

G

R

R

V

I

Q

L

L

I

L

D

G

G

R

Q

A

D

L

I

A

A

Q

H

I

V

P

T

A

Q

R

T

E

S

R

L

A

R

R

A

Q

A

L

L

A

G

W

I

L

V

G

P

Q

Q

G

D

E

S

G

V

V

L

T

F

D

N

E

D

L

T

T

I

S

G

Y

Y

D

N

I

I

A

A

M

Y

L

-

G

G

R

R

L

D

P

G

H

A

Q

S

A

R

W

A

L

E

A

V

P

D

P

A

G

A

P

A

A

K

D

G

H

A

A

A

A

I

V

A

E

D

Q

F

A

I

L

A

R

R

A

L

T

P

Q

Q

A

G

W

Y

D

D

W

T

R

E

-

V

-

G

Q

E

R

R

P

G

L

L

S

-

Q

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

L

A

L

I

A

A

R

R

A

T

L

L

A

V

V

K

Q

N

A

P

P

P

V

I

L

L

L

F

D

D

E

E

P

A

L

T

A

S

N

A

L

L

D

D

P

T

P

R

H

T

Q

E

T

Q

D

D

W

I

L

L

H

S

T

T

M

M

R

R

T

A

L

-

V

V

A

A

A

S

G

H

T

R

T

T

V

T

V

I

S

S

V

I

L

A

H

H

E

R

V

L

S

S

L

T

A

I

L

A

Q

D

A

S

D

D

D

T

M

I

V

L

L

V

M

L

A

D

A

Q

G

G

R

R

V

L

L

A

H

E

Q

Q

G

G

binding phosphate ion: S389 (≠ N41), G390 (= G42), G392 (= G44), S394 (= S46)

Sites not aligning to the query:

binding glutathione: 149, 261, 262, 265, 309, 310, 310, 311, 312, 313

4mrnA Structure of a bacterial atm1-family abc transporter (see paper)
34% identity, 76% coverage: 19:225/273 of query aligns to 367:570/600 of 4mrnA

query
sites
4mrnA

R

R

Q

E

I

I

L

L

H

H

G

G

I

L

D

S

L

F

Q

E

L

V

P

A

A

A

G

G

R

S

W

R

T

V

S

A

I

I

V

V

G

G

P

P

N
x
S

G

G

A

A

G
|
G

K
|
K

S
|
S

T

T

L

I

L

-

K

-

A

-

L

-

A

A

G

R

L

L

P

F

R

R

A

F

A

Y

V

D

-

P

-

W

Q

E

G

G

R

R

V

I

Q

L

L

I

L

D

G

G

R

Q

A

D

L

I

A

A

Q

H

I

V

P

T

A

Q

R

T

E

S

R

L

A

R

R

A

Q

A

L

L

A

G

W

I

L

V

G

P

Q

Q

G

D

E

S

G

V

V

L

T

F

D

N

E

D

L

T

T

I

S

G

Y

Y

D

N

I

I

A

A

M

Y

L

-

G

G

R

R

L

D

P

G

H

A

Q

S

A

R

W

A

L

E

A

V

P

D

P

A

G

A

P

A

A

K

D

G

H

A

A

A

A

I

V

A

E

D

Q

F

A

I

L

A

R

R

A

L

T

P

Q

Q

A

G

W

Y

D

D

W

T

R

E

-

V

-

G

Q

E

R

R

P

G

L

L

S

-

Q

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

L

A

L

I

A

A

R

R

A

T

L

L

A

V

V

K

Q

N

A

P

P

P

V

I

L

L

L

F

D

D

E

E

P

A

L

T

A

S

N

A

L

L

D

D

P

T

P

R

H

T

Q

E

T

Q

D

D

W

I

L

L

H

S

T

T

M

M

R

R

T

A

L

-

V

V

A

A

A

S

G

H

T

R

T

T

V

T

V

I

S

S

V

I

L

A

H

H

E

R

V

L

S

S

L

T

A

I

L

A

Q

D

A

S

D

D

D

T

M

I

V

L

L

V

M

L

A

D

A

Q

G

G

R

R

V

L

L

A

H

E

Q

Q

G

G

binding phosphate ion: S389 (≠ N41), G392 (= G44), K393 (= K45), S394 (= S46)

6parA Structure of a bacterial atm1-family abc exporter with mgamppnp bound (see paper)
34% identity, 76% coverage: 19:225/273 of query aligns to 353:556/578 of 6parA

query
sites
6parA

R
|
R

Q

E

I
|
I

L

L

H

H

G

G

I

L

D

S

L

F

Q

E

L

V

P

A

A

A

G

G

R

S

W

R

T

V

S

A

I

I

V

V

G

G

P

P

N
x
S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

I

L

-

K

-

A

-

L

-

A

A

G

R

L

L

P

F

R

R

A

F

A

Y

V

D

-

P

-

W

Q

E

G

G

R

R

V

I

Q

L

L

I

L

D

G

G

R

Q

A

D

L

I

A

A

Q

H

I

V

P

T

A

Q

R

T

E

S

R

L

A

R

R

A

Q

A

L

L

A

G

W

I

L

V

G

P

Q
|
Q

G

D

E

S

G

V

V

L

T

F

D

N

E

D

L

T

T

I

S

G

Y

Y

D

N

I

I

A

A

M

Y

L

-

G

G

R

R

L

D

P

G

H

A

Q

S

A

R

W

A

L

E

A

V

P

D

P

A

G

A

P

A

A

K

D

G

H

A

A

A

A

I

V

A

E

D

Q

F

A

I

L

A

R

R

A

L

T

P

Q

Q

A

G

W

Y

D

D

W

T

R

E

-

V

-

G

Q

E

R

R

P

G

L

L

S

-

Q
x
K

L

L

S
|
S

G
|
G

E
|
E

R

K

Q

Q

R

R

V

V

L

A

L

I

A

A

R

R

A

T

L

L

A

V

V

K

Q

N

A

P

P

P

V

I

L

L

L

F

D

D

E

E

P

A

L

T

A

S

N

A

L

L

D

D

P

T

P

R

H

T

Q

E

T

Q

D

D

W

I

L

L

H

S

T

T

M

M

R

R

T

A

L

-

V

V

A

A

A

S

G

H

T

R

T

T

V

T

V

I

S

S

V

I

L

A

H

H

E

R

V

L

S

S

L

T

A

I

L

A

Q

D

A

S

D

D

D

T

M

I

V

L

L

V

M

L

A

D

A

Q

G

G

R

R

V

L

L

A

H

E

Q

Q

G

G

binding phosphoaminophosphonic acid-adenylate ester: R353 (= R19), I355 (= I21), S375 (≠ N41), G376 (= G42), G378 (= G44), K379 (= K45), S380 (= S46), T381 (= T47), K476 (≠ Q144), S478 (= S146), G479 (= G147), G480 (= G148), E481 (= E149)
binding magnesium ion: S380 (= S46), Q421 (= Q89)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 349

6pamA Structure of a bacterial atm1-family abc transporter with mgadp bound (see paper)
34% identity, 76% coverage: 19:225/273 of query aligns to 362:565/586 of 6pamA

query
sites
6pamA

R
|
R

Q

E

I

I

L

L

H

H

G

G

I

L

D

S

L

F

Q

E

L

V

P

A

A

A

G

G

R

S

W

R

T

V

S

A

I

I

V

V

G

G

P

P

N
x
S

G

G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

I

L

-

K

-

A

-

L

-

A

A

G

R

L

L

P

F

R

R

A

F

A

Y

V

D

-

P

-

W

Q

E

G

G

R

R

V

I

Q

L

L

I

L

D

G

G

R

Q

A

D

L

I

A

A

Q

H

I

V

P

T

A

Q

R

T

E

S

R

L

A

R

R

A

Q

A

L

L

A

G

W

I

L

V

G

P

Q

Q

G

D

E

S

G

V

V

L

T

F

D

N

E

D

L

T

T

I

S

G

Y

Y

D

N

I

I

A

A

M

Y

L

-

G

G

R

R

L

D

P

G

H

A

Q

S

A

R

W

A

L

E

A

V

P

D

P

A

G

A

P

A

A

K

D

G

H

A

A

A

A

I

V

A

E

D

Q

F

A

I

L

A

R

R

A

L

T

P

Q

Q

A

G

W

Y

D

D

W

T

R

E

-

V

-

G

Q

E

R

R

P

G

L

L

S

-

Q

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

L

A

L

I

A

A

R

R

A

T

L

L

A

V

V

K

Q

N

A

P

P

P

V

I

L

L

L

F

D
|
D

E

E

P

A

L

T

A

S

N

C

L

L

D

D

P

T

P

R

H

T

Q

E

T

Q

D

D

W

I

L

L

H

S

T

T

M

M

R

R

T

A

L

-

V

V

A

A

A

S

G

H

T

R

T

T

V

T

V

I

S

S

V

I

L

A

H

H

E

R

V

L

S

S

L

T

A

I

L

A

Q

D

A

S

D

D

D

T

M

I

V

L

L

V

M

L

A

D

A

Q

G

G

R

R

V

L

L

A

H

E

Q

Q

G

G

binding adenosine-5'-diphosphate: R362 (= R19), S384 (≠ N41), G387 (= G44), K388 (= K45), S389 (= S46), T390 (= T47)
binding magnesium ion: S389 (= S46), D510 (= D169)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 120, 358

Q2G506 ATM1-type heavy metal exporter; ATP-binding cassette transporter Atm1; NaAtm1; EC 7.-.-.- from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CCUG 56034 / CIP 105152 / NBRC 16084 / F199) (see paper)
34% identity, 76% coverage: 19:225/273 of query aligns to 374:577/608 of Q2G506

query
sites
Q2G506

R

R

Q

E

I

I

L

L

H

H

G

G

I

L

D

S

L

F

Q

E

L

V

P

A

A

A

G

G

R

S

W

R

T

V

S

A

I

I

V

V

G

G

P

P

N

S

G

G

A

A

G

G

K

K

S

S

T

T

L

I

L

-

K

-

A

-

L

-

A

A

G

R

L

L

P

F

R

R

A

F

A

Y

V

D

-

P

-

W

Q

E

G

G

R

R

V

I

Q

L

L

I

L

D

G

G

R

Q

A

D

L

I

A

A

Q

H

I

V

P

T

A

Q

R

T

E

S

R

L

A

R

R

A

Q

A

L

L

A

G

W

I

L

V

G

P

Q

Q

G

D

E

S

G

V

V

L

T

F

D

N

E

D

L

T

T

I

S

G

Y

Y

D

N

I

I

A

A

M

Y

L

-

G

G

R

R

L

D

P

G

H

A

Q

S

A

R

W

A

L

E

A

V

P

D

P

A

G

A

P

A

A

K

D

G

H

A

A

A

A

I

V

A

E

D

Q

F

A

I

L

A

R

R

A

L

T

P

Q

Q

A

G

W

Y

D

D

W

T

R

E

-

V

-

G

Q

E

R

R

P

G

L

L

S

-

Q

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

L

A

L

I

A

A

R

R

A

T

L

L

A

V

V

K

Q

N

A

P

P

P

V

I

L

L

L

F

D

D

E
|
E

P

A

L

T

A

S

N

A

L

L

D

D

P

T

P

R

H

T

Q

E

T

Q

D

D

W

I

L

L

H

S

T

T

M

M

R

R

T

A

L

-

V

V

A

A

A

S

G

H

T

R

T

T

V

T

V

I

S

S

V

I

L

A

H

H

E

R

V

L

S

S

L

T

A

I

L

A

Q

D

A

S

D

D

D

T

M

I

V

L

L

V

M

L

A

D

A

Q

G

G

R

R

V

L

L

A

H

E

Q

Q

G

G

E523 (= E170) mutation to Q: Abolishes transporter activity.

Sites not aligning to the query:

195 Y→F: Strongly increases basal rate of ATP hydrolysis.
269 N→A: Increases basal rate of ATP hydrolysis and abolishes stimulation of ATP hydrolysis by glutathione.
269:272 binding glutathione
272 Q→A: Abolishes glutathione-dependent ATP hydrolysis.
316:319 binding glutathione
319 G→L: Abolishes glutathione-dependent ATP hydrolysis.

5x40A Structure of a cbio dimer bound with amppcp (see paper)
38% identity, 78% coverage: 17:229/273 of query aligns to 16:226/280 of 5x40A

query
sites
5x40A

G

G

A

G

R
x
V

Q

K

I
x
A

L

L

H

D

G

D

I

L

D

S

L

L

Q

A

L

V

P

P

A

K

G

G

R

E

W

S

T

L

S

A

I

I

V

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

L

A

H

L

L

A

N

G

G

L

T

L

L

-

R

P

P

R

Q

A

S

A

-

V

-

Q

-

G

G

R

R

V

V

Q

L

L

L

L

G

G

G

R

T

A

A

L

T

A

G

Q

H

I

-

P

-

A

S

R

R

E

K

-

D

-

L

-

T

-

G

-

W

R

R

A

R

R

R

Q

V

L

G

A

L

W

V

L

L

G
x
Q

Q

D

G

A

E

D

G

D

V

Q

T

L

D

F

E

A

L

T

T

T

S

V

Y

F

D

E

I

D

A

V

M

S

L

F

G

G

R

P

L

L

P

N

H

-

Q

-

A

-

W

-

L

L

A

G

P

L

P

S

G

E

P

A

A

E

D

A

H

R

A

A

A

R

V

V

E

E

Q

E

A

A

L

L

R

A

A

A

T

L

Q

S

A

I

W

S

D

D

W

L

R

R

Q

D

R

R

P

P

L

T

S
x
H

Q
x
M

L
|
L

S
|
S

G

G

G
|
G

E
x
Q

R

K

Q

R

R

R

V

V

L

A

L

I

A

A

R

G

A

A

L

V

A

A

V

M

Q

R

A

P

P

E

V

V

L

L

D

D

E
x
Q

P

P

L

T

A

A

N

G

L

L

D

D

P

L

P

A

H

G

Q

T

T

E

D

Q

W

L

L

L

H

T

T

L

M

L

R

R

T

G

L

L

V

R

A

A

G

G

T

M

T

T

V

L

V

V

S

F

V

S

L

T

H
|
H

E

D

V

V

S

E

L

L

A

A

L

A

Q

A

-

L

A

A

D

D

D

R

M

V

V

A

L

L

M

F

A

R

A

T

G

G

R

R

V

V

L

L

H

A

Q

E

G

G

R

A

C

A

T

E

A

A

binding phosphomethylphosphonic acid adenylate ester: V18 (≠ R19), A20 (≠ I21), N40 (= N41), G41 (= G42), G43 (= G44), K44 (= K45), S45 (= S46), T46 (= T47), Q88 (≠ G88), H139 (≠ S143), M140 (≠ Q144), L141 (= L145), S142 (= S146), G144 (= G148), Q145 (≠ E149), Q166 (≠ E170), H198 (= H202)
binding magnesium ion: S45 (= S46), Q88 (≠ G88)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 14

Q61102 Iron-sulfur clusters transporter ABCB7, mitochondrial; ATP-binding cassette sub-family B member 7, mitochondrial; ATP-binding cassette transporter 7; ABC transporter 7 protein from Mus musculus (Mouse) (see paper)
30% identity, 77% coverage: 16:225/273 of query aligns to 482:688/752 of Q61102

query
sites
Q61102

I

I

G

E

A

G

R

Q

Q

K

I

V

L

L

H

N

G

G

I

V

D

S

L

F

Q

E

L

V

P

P

A

A

G

G

R

K

W

K

T

V

S

A

I

I

V

V

G

G

P

G

N

S

G

G

A

S

G

G

K

K

S

S

T

T

L

I

L

V

K

R

A

L

L

L

A

F

G

R

L

F

L

Y

-

E

P

P

R

Q

A

-

V

-

Q

K

G

G

R

S

V

I

Q

Y

L

L

L

A

G

G

R

Q

A

N

L

L

A

Q

Q

D

I

V

P

S

A

L

R

E

E

S

R

L

A

R

R

R

Q

A

L

V

A

G

W

V

L

V

G

P

Q

Q

G

-

E

D

G

A

V

V

T

L

D

F

E

H

L

N

T

T

S

I

Y

Y

D

Y

I

N

A

L

M

L

L

Y

G

G

R

N

L

I

-

N

-

A

-

S

P

P

H

E

Q

E

A

V

W

Y

L

A

A

V

P

A

P

K

G

L

P

A

A

G

D

L

H

H

A

D

A

A

V

I

E

-

Q

-

A

-

L

L

R

R

A

M

T

P

Q

H

A

G

W

Y

D

D

W

T

R

Q

-

V

-

G

Q

E

R

R

P

G

L

L

S

-

Q

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

L

A

L

I

A

A

R

R

A

A

L

I

A

L

V

K

Q

N

A

P

P

P

V

V

L

I

L

L

L

Y

D

D

E

E

P

A

L

T

A

S

N

S

L

L

D

D

P

S

P

I

H

T

Q

E

T

E

D

T

W

I

L

L

H

G

T

A

M

M

R

R

T

D

L

V

V

V

A

K

A

H

G

R

T

T

T

S

V

I

V

F

S

-

V

I

L

A

H

H

E

R

V

L

S

S

L

T

A

V

L

V

Q

D

A

A

D

D

D

E

M

I

V

I

L

V

M

L

A

S

A

Q

G

G

R

K

V

V

L

A

H

E

Q

R

G

G

Sites not aligning to the query:

433 E→K: Exhibits siderocytosis with increased zinc protoporphyrin.

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
30% identity, 77% coverage: 16:225/273 of query aligns to 27:230/378 of P69874

query
sites
P69874

I
x
F

G

D

A

G

R

K

Q

E

I

V

L

I

H

P

G

Q

I

L

D

D

L

L

Q

T

L

I

P

N

A

N

G

G

R

E

W
x
F

T

L

S

T

I

L

V

L

G

G

P

P

N

S

G

G

A
x
C

G

G

K

K

S

T

T

T

L

V

L
|
L

K

R

A

L

L

I

A

A

G

G

L

L

L

-

P

-

R

E

A

T

A

V

V

D

Q

S

G

G

R

R

V

I

Q

M

L
|
L

L

D

G

N

R

E

A

D

L

I

A

T

Q

H

I

V

P

P

A

A

R

E

E

N

R

R

A

Y

R

V

Q

N

L

T

A

V

W

F

L

-

G

-

Q

Q

G

S

E

Y

G

A

V

L

T

F

D

P

E

H

L

M

T

T

S

V

Y

F

D

E

I

N

A

V

M

A

L

F

G

G

R

L

L

R

P

M

H

Q

Q

K

A

T

W

P

L

A

A

A

P

E

P

I

G

T

P

P

A

R

D

-

H

-

A

-

V

V

E

M

Q

E

A

A

L

L

R

R

A

M

T
x
V

Q

Q

A

L

W

E

D

T

W

F

R

A

Q

Q

R

R

P

K

L

P

S

H

Q

Q

L

L

S

S

G

G

E

Q

R

Q

Q

Q

R

R

V

V

L

A

L

I

A

A

R

R

A

A

L

V

A

V

V

N

Q

K

A

P

P

R

V

L

L

L

D
|
D

E

E

P

S

L

L

A

S

N

A

L

L

D

D

P

Y

P

K

H

L

Q

R

T

K

D

Q

W

M

L

Q

H

N

T

E

M

L

R

K

T

A

L

L

V

Q

A

R

A

K

-

L

G

G

T

I

T

T

V

F

V

V

S

F

V

V

L

T

H

H

E

D

V

Q

S

E

L

E

A

A

L

L

-

T

Q

M

A

S

D

D

D

R

M

I

V

V

L

V

M

M

A

R

A

D

G

G

R

R

V

I

L

E

H

Q

Q

D

G

G

F27 (≠ I16) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ W34) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ A43) mutation to T: Loss of ATPase activity and transport.
L60 (= L49) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L67) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ T132) mutation to M: Loss of ATPase activity and transport.
D172 (= D169) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

26 C→A: Lower ATPase activity and transport efficiency.
276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

H2LNR5 Iron-sulfur clusters transporter ABCB7, mitochondrial; ATP-binding cassette sub-family B member 7, mitochondrial from Oryzias latipes (Japanese rice fish) (Japanese killifish) (see paper)
28% identity, 80% coverage: 19:236/273 of query aligns to 478:686/746 of H2LNR5

query
sites
H2LNR5

R

Q

Q

K

I

V

L

L

H

N

G

G

I

V

D

S

L

F

Q

E

L

V

P

P

A

A

G

G

R

K

W

K

T

V

S

A

I

I

V

V

G

G

P

G

N

S

G

G

A

S

G

G

K

K

S

S

T

T

L

I

L

V

K

R

A

L

L

L

A

F

G

R

L

F

L

Y

-

E

P

P

R

Q

A

-

V

-

Q

Q

G

G

R

N

V

I

Q

Y

L

I

L

S

G

G

R

Q

A

N

L

I

A

R

Q

D

I

V

P

S

A

L

R

E

E

S

R

L

A

R

R

K

Q

A

L

L

A

G

W

V

L

V

G

P

Q

Q

G

D

E

A

G

V

V

L

T

F

D

H

E

N

L

N

T

I

S

F

Y

Y

D

N

I

L

A

Q

M

Y

-

G

-

N

L

I

G

N

R

A

L

T

P

P

H

E

Q

E

A

V

W

Y

L

Q

A

V

P

A

P

R

G

L

P

A

A

G

D

L

H

H

A

D

A

A

V

I

E

-

Q

-

A

-

L

L

R

R

A

M

T

P

Q

H

A

G

W

Y

D

D

W

T

R

Q

-

V

-

G

Q

E

R

R

P

G

L

L

S

-

Q

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

L

A

L

I

A

A

R

R

A

A

L

I

A

L

V

K

Q

N

A

P

P

P

V

I

L

L

L

Y

D

D

E

E

P

A

L

T

A

S

N

S

L

L

D

D

P

S

P

I

H

T

Q

E

T

E

D

N

W

I

L

M

H

T

T

S

M

M

R

K

T

E

L

M

V

V

A

K

A

D

G

R

T

T

T

S

V

V

V

F

S

-

V

I

L

A

H

H

E

R

V

L

S

S

L

T

A

I

L

V

Q

D

A

A

D

D

D

E

M

I

V

L

L

V

M

L

A

S

A

Q

G

G

R

K

V

V

L

A

H

E

Q

R

G

G

R

-

C

-

T

-

A

-

P

-

A

-

T

T

H

H

A

Q

A

A

L

L

Sites not aligning to the query:

222 V→D: In namako (nmk) mutant. Nmk homozygous mutants exhibit steatosis and die several days after hatching. The liver of nmk mutant develops normally until the hatching stage (10 dpf) but by 2 dph, it exhibits a deformed morphology and loss of transparency. Hepatic cells are haphazardly arranged and contain a lot of vacuoles where neutral lipids are abnormally accumulated. Furthermore, mitochondoria in mutant livers tend to be enlarged and swollen.

7vgfA Cryo-em structure of amp-pnp bound human abcb7
29% identity, 77% coverage: 16:225/273 of query aligns to 338:544/550 of 7vgfA

query
sites
7vgfA

I

I

G

E

A

G

R

Q

Q

K

I

V

L

L

H

S

G

G

I

I

D

S

L

F

Q

E

L

V

P

P

A

A

G

G

R

K

W

K

T

V

S

A

I

I

V

V

G

G

P

G

N

S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T

T

L

I

L

V

K

R

A

L

L

L

A

F

G

R

L

F

L

Y

-

E

P

P

R

Q

A

-

V

-

Q

K

G

G

R

S

V

I

Q

Y

L

L

L

A

G

G

R

Q

A

N

L

I

A

Q

Q

D

I

V

P

S

A

L

R

E

E

S

R

L

A

R

R

R

Q

A

L

V

A

G

W

V

L

V

G

P

Q
|
Q

G

-

E

D

G

A

V

V

T

L

D

F

E

H

L

N

T

T

S

I

Y

Y

D

Y

I

N

A

L

M

L

L

Y

G

G

R

N

L

I

-

S

-

A

-

S

P

P

H

E

Q

E

A

V

W

Y

L

A

A

V

P

A

P

K

G

L

P

A

A

G

D

L

H

H

A

D

A

A

V

I

E

-

Q

-

A

-

L

L

R

R

A

M

T

P

Q

H

A

G

W

Y

D

D

W

T

R

Q

-

V

-

G

Q

E

R

R

P

G

L

L

S

-

Q

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

L

A

L

I

A

A

R

R

A

A

L

I

A

L

V

K

Q

D

A

P

P

P

V

V

L

I

L

L

L

Y

D

D

E

E

P

A

L
x
T

A

S

N

S

L

L

D

D

P

S

P

I

H

T

Q

E

T

E

D

T

W

I

L

L

H

G

T

A

M

M

R

K

T

D

L

V

V

V

A

K

A

H

G

R

T

T

T

S

V

I

V

F

S

-

V

I

L

A

H

H

E

R

V

L

S

S

L

T

A

V

L

V

Q

D

A

A

D

D

D

E

M

I

V

I

L

V

M

L

A

D

A

Q

G

G

R

K

V

V

L

A

H

E

Q

R

G

G

binding phosphoaminophosphonic acid-adenylate ester: G364 (= G42), G366 (= G44), K367 (= K45), S368 (= S46), Q409 (= Q89), T492 (≠ L172)
binding magnesium ion: S368 (= S46), Q409 (= Q89)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 337

O75027 Iron-sulfur clusters transporter ABCB7, mitochondrial; ATP-binding cassette sub-family B member 7, mitochondrial; ATP-binding cassette transporter 7; ABC transporter 7 protein from Homo sapiens (Human) (see 4 papers)
29% identity, 77% coverage: 16:225/273 of query aligns to 482:688/752 of O75027

query
sites
O75027

I

I

G

E

A

G

R

Q

Q

K

I

V

L

L

H

S

G

G

I

I

D

S

L

F

Q

E

L

V

P

P

A

A

G

G

R

K

W

K

T

V

S

A

I

I

V

V

G

G

P

G

N

S

G

G

A

S

G

G

K

K

S

S

T

T

L

I

L

V

K

R

A

L

L

L

A

F

G

R

L

F

L

Y

-

E

P

P

R

Q

A

-

V

-

Q

K

G

G

R

S

V

I

Q

Y

L

L

L

A

G

G

R

Q

A

N

L

I

A

Q

Q

D

I

V

P

S

A

L

R

E

E

S

R

L

A

R

R

R

Q

A

L

V

A

G

W

V

L

V

G

P

Q

Q

G

-

E

D

G

A

V

V

T

L

D

F

E

H

L

N

T

T

S

I

Y

Y

D

Y

I

N

A

L

M

L

L

Y

G

G

R

N

L

I

-

S

-

A

-

S

P

P

H

E

Q

E

A

V

W

Y

L

A

A

V

P

A

P

K

G

L

P

A

A

G

D

L

H

H

A

D

A

A

V

I

E

-

Q

-

A

-

L

L

R

R

A

M

T

P

Q

H

A

G

W

Y

D

D

W

T

R

Q

-

V

-

G

Q

E

R

R

P

G

L

L

S

-

Q

K

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

V

L

A

L

I

A

A

R

R

A

A

L

I

A

L

V

K

Q

D

A

P

P

P

V

V

L

I

L

L

L

Y

D

D

E

E

P

A

L

T

A

S

N

S

L

L

D

D

P

S

P

I

H

T

Q

E

T

E

D

T

W

I

L

L

H

G

T

A

M

M

R

K

T

D

L

V

V

V

A

K

A

H

G

R

T

T

T

S

V

I

V

F

S

-

V

I

L

A

H

H

E

R

V

L

S

S

L

T

A

V

L

V

Q

D

A

A

D

D

D

E

M

I

V

I

L

V

M

L

A

D

A

Q

G

G

R

K

V

V

L

A

H

E

Q

R

G

G

Sites not aligning to the query:

315 natural variant: R -> G
346 natural variant: F -> I
433 E → K: in ASAT; impaired maturation of cytosolic Fe/S proteins, loss of the ability to couple MgATP binding with stimulation of ATPase activity at the nucleotide binding domain;; loss of [2Fe-2S]-(GS)4 cluster transport; dbSNP:rs80356714; E→D: Significantly increases ATPase activity by [2Fe-2S]-(GS)4 cluster stimulation. Increases affinity for Mg-ATP. Does not affect affinity for Mg-ATP in the presence of the in the presence of [2Fe-2S]-(GS)4 cluster. Does not affect [2Fe-2S]-(GS)4 cluster transport.; E→K: Loss of ATPase activity stimulation by [2Fe-2S]-(GS)4 cluster stimulation. Loss of the ability to couple MgATP binding with stimulation of ATPase activity at the nucleotide binding domain. Loss of [2Fe-2S]-(GS)4 cluster transport.; E→Q: Loss of ATPase activity stimulation by [2Fe-2S]-(GS)4 cluster stimulation. Loss of the ability to couple MgATP binding with stimulation of ATPase activity at the nucleotide binding domain. Loss of [2Fe-2S]-(GS)4 cluster transport.

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
33% identity, 74% coverage: 20:222/273 of query aligns to 22:222/650 of 5ws4A

query
sites
5ws4A

Q

Q

I

I

L

L

H

K

G

G

I

I

D

D

L

L

Q

T

L

I

P

Y

A

E

G

G

R

E

W

L

T

V

S

A

I

I

V

V

G

G

P

Q

N
x
S

G
|
G

A

S

G

G

K

K

S
|
S

T

T

L

L

L

M

K

N

A

I

L

L

A

-

G

G

L

C

L

L

P

D

R

R

A

P

A

T

V

-

Q

S

G

G

R

S

V

Y

Q

K

L

V

L

N

G

G

R

Q

A

E

L

T

A

G

Q

K

I

L

P

E

-

P

-

D

-

Q

-

L

A

A

R

Q

E

L

R

R

A

R

R

E

Q

Y

L

F

A

G

W

F

L

I

G

F

Q

Q

G

R

E

Y

G

H

V

L

T

L

D

G

E

D

L

L

T

S

S

A

Y

E

D

-

I

-

A

-

M

-

L

-

G

G

R

N

L

V

P

E

H

V

Q

P

A

A

W

V

L

Y

A

A

P

G

P

V

G

T

P

P

A

A

D

D

H

R

A

K

A

Q

V

R

E

A

Q

T

A

A

L

L

R

L

A

T

T

E

Q

L

A

G

W

L

D

G

W

T

R

K

-

T

-

Q

Q

N

R

R

P

P

L

-

S

S

Q

Q

L

L

S

S

G

G

E

Q

R

Q

Q

Q

R

R

V

V

L

S

L

I

A

A

R

R

A

A

L

L

A

M

V

N

Q

G

A

G

P

D

V

V

L

I

L

L

L

A

D

D

E

E

P

P

L

T

A

G

N

A

L

L

D

D

P

S

P

H

H

S

Q

G

T

V

D

E

W

V

L

M

H

R

T

I

M

L

R

R

T

E

L

L

V

N

A

A

G

G

T

H

T

T

V

I

S

L

V

V

L

T

H

H

E

D

V

M

S

Q

L

V

A

A

L

K

Q

N

A

A

D

T

D

R

M

I

V

I

L

E

M

I

A

S

A

D

G

G

R

E

V

I

L

I

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (≠ N41), G44 (= G42), S48 (= S46)

A0R6H8 Mycobactin import ATP-binding/permease protein IrtA; EC 7.2.2.- from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
32% identity, 75% coverage: 21:225/273 of query aligns to 624:824/860 of A0R6H8

query
sites
A0R6H8

I

V

L

L

H

H

G

D

I

I

D

S

L

L

Q

Q

L

L

P

T

A

P

G

G

R

T

W

V

T

T

S

A

I

L

V

V

G

G

P

P

N

S

G

G

A

S

G

G

K

K

S

S

T

T

L

L

L

A

K

A

A

L

L

L

A

A

G

R

L

F

L

H

P

D

R

V

A

D

A

A

V

-

Q

-

G

G

R

A

V

I

Q

R

L

L

L

G

G

G

R

R

A

D

L

I

A

R

Q

T

I

L

P

T

A

A

R

D

E

E

R

L

A

Y

R

R

Q

Q

L

V

A

G

W

F

L

V

G

L

Q

Q

G

D

E

T

G

Q

V

L

T

V

D

G

E

G

L

T

T

V

S

A

Y

E

D

N

I

I

A

A

M

L

L

A

G

D

R

-

L

-

P

P

H

D

Q

A

A

S

W

I

L

E

A

R

P

I

P

Q

G

D

P

A

A

A

D

R

H

D

A

A

A

Q

V

I

-

H

E

D

Q

R

A

I

L

M

R

R

A

L

T

P

Q

N

A

G

W

Y

D

D

W

T

R

P

Q

L

R

G

P

A

L

A

S

S

Q

S

L

L

S

S

G

G

E

E

R

K

Q

Q

R

R

V

L

L

T

L

I

A

A

R

R

A

A

L

I

A

L

V

A

Q

D

A

T

P

P

V

V

L

L

L

I

L

L

D

D

E

E

P

A

L

T

A

A

N

F

L

A

D

D

P

P

P

E

H

S

Q

E

T

Y

D

L

W

V

L

Q

H

Q

T

A

M

L

R

N

T

R

L

L

V

T

A

-

A

R

G

D

T

R

T

T

V

V

V

L

S

V

V

I

L

A

H

H

E

R

V

L

S

H

L

T

A

I

L

T

Q

H

A

A

D

D

D

Q

M

I

V

V

L

V

M

L

A

E

A

G

G

G

R

R

V

I

L

V

H

E

Q

T

G

G

Sites not aligning to the query:

55 R→E: Cannot reduce Fe(III)-cMBT and Fe(III)-MBT; when associated with E-234 and E-241.
234 Q→E: Cannot reduce Fe(III)-cMBT and Fe(III)-MBT; when associated with E-55 and E-241.
241 R→E: Cannot reduce Fe(III)-cMBT and Fe(III)-MBT; when associated with E-55 and E-234.

Query Sequence

>WP_049774007.1 NCBI__GCF_000015565.1:WP_049774007.1
MKNPIALGASQLSARIGARQILHGIDLQLPAGRWTSIVGPNGAGKSTLLKALAGLLPRAA
VQGRVQLLGRALAQIPARERARQLAWLGQGEGVTDELTSYDIAMLGRLPHQAWLAPPGPA
DHAAVEQALRATQAWDWRQRPLSQLSGGERQRVLLARALAVQAPVLLLDEPLANLDPPHQ
TDWLHTMRTLVAAGTTVVSVLHEVSLALQADDMVLMAAGRVLHQGRCTAPATHAALEQVF
GQRILVRHLQGMWLALPALGGGQYTPPDTPPGV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory