SitesBLAST
Comparing WP_049776088.1 NCBI__GCF_000017645.1:WP_049776088.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
50% identity, 99% coverage: 1:256/258 of query aligns to 4:257/257 of 6slbAAA
- active site: Q64 (= Q60), F69 (vs. gap), L80 (≠ I78), N84 (= N83), A108 (= A107), S111 (≠ N110), A130 (= A129), F131 (= F130), L136 (= L135), P138 (= P137), D139 (= D138), A224 (≠ D223), G234 (= G233)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (= R54), A62 (= A58), Q64 (= Q60), D65 (= D61), L66 (= L62), Y76 (≠ L74), A108 (= A107), F131 (= F130), D139 (= D138)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
48% identity, 99% coverage: 1:256/258 of query aligns to 1:245/245 of 6slaAAA
- active site: Q61 (= Q60), L68 (≠ K67), N72 (= N83), A96 (= A107), S99 (≠ N110), A118 (= A129), F119 (= F130), L124 (= L135), P126 (= P137), N127 (≠ D138), A212 (≠ D223), G222 (= G233)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (= L21), A59 (= A58), Q61 (= Q60), D62 (= D61), L63 (= L62), L68 (≠ K67), Y71 (= Y82), A94 (= A105), G95 (= G106), A96 (= A107), F119 (= F130), I122 (= I133), L124 (= L135), N127 (≠ D138), F234 (= F245), K237 (= K248)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
36% identity, 96% coverage: 10:256/258 of query aligns to 16:259/259 of 5zaiC
- active site: A65 (≠ Q60), F70 (≠ L72), S82 (vs. gap), R86 (≠ N83), G110 (≠ A107), E113 (≠ N110), P132 (≠ A129), E133 (≠ F130), I138 (≠ L135), P140 (= P137), G141 (≠ D138), A226 (≠ D223), F236 (≠ G233)
- binding coenzyme a: K24 (≠ R20), L25 (= L21), A63 (= A58), G64 (= G59), A65 (≠ Q60), D66 (= D61), I67 (≠ L62), P132 (≠ A129), R166 (≠ P163), F248 (= F245), K251 (= K248)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
34% identity, 95% coverage: 13:256/258 of query aligns to 16:256/256 of 3h81A
- active site: A64 (≠ Q60), M69 (≠ R65), T79 (≠ E79), F83 (≠ N83), G107 (≠ A107), E110 (≠ N110), P129 (≠ A129), E130 (≠ F130), V135 (≠ L135), P137 (= P137), G138 (≠ D138), L223 (≠ D223), F233 (≠ G233)
- binding calcium ion: F233 (≠ G233), Q238 (≠ Y238)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
33% identity, 98% coverage: 2:254/258 of query aligns to 6:255/255 of 3q0jC
- active site: A65 (≠ Q60), M70 (≠ R65), T80 (≠ E79), F84 (≠ N83), G108 (≠ A107), E111 (≠ N110), P130 (≠ A129), E131 (≠ F130), V136 (≠ L135), P138 (= P137), G139 (≠ D138), L224 (≠ D223), F234 (≠ G233)
- binding acetoacetyl-coenzyme a: Q23 (≠ D19), A24 (≠ R20), L25 (= L21), A27 (= A23), A63 (= A58), G64 (= G59), A65 (≠ Q60), D66 (= D61), I67 (≠ L62), K68 (≠ G63), M70 (≠ R65), F84 (≠ N83), G107 (= G106), G108 (≠ A107), E111 (≠ N110), P130 (≠ A129), E131 (≠ F130), P138 (= P137), G139 (≠ D138), M140 (≠ S139)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
33% identity, 98% coverage: 2:254/258 of query aligns to 6:255/255 of 3q0gC
- active site: A65 (≠ Q60), M70 (≠ R65), T80 (≠ E79), F84 (≠ N83), G108 (≠ A107), E111 (≠ N110), P130 (≠ A129), E131 (≠ F130), V136 (≠ L135), P138 (= P137), G139 (≠ D138), L224 (≠ D223), F234 (≠ G233)
- binding coenzyme a: L25 (= L21), A63 (= A58), I67 (≠ L62), K68 (≠ G63), Y104 (≠ V103), P130 (≠ A129), E131 (≠ F130), L134 (≠ I133)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
35% identity, 93% coverage: 13:253/258 of query aligns to 20:255/258 of 1mj3A
- active site: A68 (≠ Q60), M73 (≠ R65), S83 (≠ N83), L85 (= L85), G109 (≠ A107), E112 (≠ N110), P131 (≠ A129), E132 (≠ F130), T137 (≠ L135), P139 (= P137), G140 (≠ D138), K225 (≠ D223), F235 (≠ G233)
- binding hexanoyl-coenzyme a: K26 (≠ D19), A27 (≠ R20), L28 (= L21), A30 (= A23), A66 (= A58), G67 (= G59), A68 (≠ Q60), D69 (= D61), I70 (≠ L62), G109 (≠ A107), P131 (≠ A129), E132 (≠ F130), L135 (≠ I133), G140 (≠ D138)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
35% identity, 93% coverage: 13:253/258 of query aligns to 19:251/254 of 2dubA
- active site: A67 (≠ Q60), M72 (≠ R65), S82 (≠ N83), G105 (≠ A107), E108 (≠ N110), P127 (≠ A129), E128 (≠ F130), T133 (≠ L135), P135 (= P137), G136 (≠ D138), K221 (≠ D223), F231 (≠ G233)
- binding octanoyl-coenzyme a: K25 (≠ D19), A26 (≠ R20), L27 (= L21), A29 (= A23), A65 (= A58), A67 (≠ Q60), D68 (= D61), I69 (≠ L62), K70 (≠ G63), G105 (≠ A107), E108 (≠ N110), P127 (≠ A129), E128 (≠ F130), G136 (≠ D138), A137 (≠ S139)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
34% identity, 93% coverage: 13:253/258 of query aligns to 18:255/258 of 1ey3A
- active site: A66 (≠ Q60), M71 (≠ R65), S81 (≠ G75), L85 (≠ E79), G109 (≠ A107), E112 (≠ N110), P131 (≠ A129), E132 (≠ F130), T137 (≠ L135), P139 (= P137), G140 (≠ D138), K225 (≠ D223), F235 (≠ G233)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ D19), L26 (= L21), A28 (= A23), A64 (= A58), G65 (= G59), A66 (≠ Q60), D67 (= D61), I68 (≠ L62), L85 (≠ E79), W88 (≠ Y82), G109 (≠ A107), P131 (≠ A129), L135 (≠ I133), G140 (≠ D138)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
34% identity, 93% coverage: 13:253/258 of query aligns to 20:257/260 of 1dubA
- active site: A68 (≠ Q60), M73 (≠ R65), S83 (≠ G75), L87 (≠ E79), G111 (≠ A107), E114 (≠ N110), P133 (≠ A129), E134 (≠ F130), T139 (≠ L135), P141 (= P137), G142 (≠ D138), K227 (≠ D223), F237 (≠ G233)
- binding acetoacetyl-coenzyme a: K26 (≠ D19), A27 (≠ R20), L28 (= L21), A30 (= A23), A66 (= A58), A68 (≠ Q60), D69 (= D61), I70 (≠ L62), Y107 (≠ V103), G110 (= G106), G111 (≠ A107), E114 (≠ N110), P133 (≠ A129), E134 (≠ F130), L137 (≠ I133), G142 (≠ D138), F233 (≠ Q229), F249 (= F245)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
34% identity, 93% coverage: 13:253/258 of query aligns to 50:287/290 of P14604
- E144 (≠ N110) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (≠ F130) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
34% identity, 93% coverage: 13:253/258 of query aligns to 20:257/260 of 2hw5C
- active site: A68 (≠ Q60), M73 (≠ E69), S83 (≠ E79), L87 (≠ N83), G111 (≠ A107), E114 (≠ N110), P133 (≠ A129), E134 (≠ F130), T139 (≠ L135), P141 (= P137), G142 (≠ D138), K227 (≠ D223), F237 (≠ G233)
- binding crotonyl coenzyme a: K26 (≠ D19), A27 (≠ R20), L28 (= L21), A30 (= A23), K62 (≠ R54), I70 (≠ L62), F109 (≠ A105)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
34% identity, 94% coverage: 13:254/258 of query aligns to 16:250/250 of 3q0gD
- active site: A64 (≠ Q60), M69 (≠ R65), T75 (≠ P71), F79 (≠ G75), G103 (≠ A107), E106 (≠ N110), P125 (≠ A129), E126 (≠ F130), V131 (≠ L135), P133 (= P137), G134 (≠ D138), L219 (≠ D223), F229 (≠ G233)
- binding Butyryl Coenzyme A: F225 (≠ Q229), F241 (= F245)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
35% identity, 96% coverage: 9:256/258 of query aligns to 13:261/261 of 5jbxB
- active site: A67 (≠ Q60), R72 (= R65), L84 (≠ Y82), R88 (≠ I86), G112 (≠ A107), E115 (≠ N110), T134 (≠ A129), E135 (≠ F130), I140 (≠ L135), P142 (= P137), G143 (≠ D138), A228 (≠ D223), L238 (≠ G233)
- binding coenzyme a: S24 (≠ D19), R25 (= R20), R26 (≠ L21), A28 (= A23), A65 (= A58), D68 (= D61), L69 (= L62), K70 (≠ G63), L110 (≠ A105), G111 (= G106), T134 (≠ A129), E135 (≠ F130), L138 (≠ I133), R168 (≠ P163)
7borA Structure of pseudomonas aeruginosa coa-bound odaa (see paper)
33% identity, 96% coverage: 1:248/258 of query aligns to 3:247/247 of 7borA
- active site: N63 (≠ Q60), F68 (≠ K67), D77 (≠ G75), G81 (≠ E79), I105 (≠ A107), T108 (≠ N110), F128 (= F130), L133 (= L135), P135 (= P137), E136 (≠ D138), A222 (≠ D223), L232 (≠ G233)
- binding coenzyme a: D21 (= D19), K22 (≠ R20), A25 (= A23), S61 (≠ A58), I65 (≠ L62), V103 (≠ A105), F128 (= F130), L131 (≠ I133), F244 (= F245), R247 (≠ K248)
5dufA Crystal structure of m. Tuberculosis echa6 bound to ligand gsk729a (see paper)
35% identity, 99% coverage: 1:255/258 of query aligns to 3:244/245 of 5dufA
- active site: A62 (≠ Q60), D67 (= D64), P74 (= P71), I78 (= I78), A102 (= A107), Q105 (≠ N110), P124 (≠ A129), T125 (≠ F130), L130 (= L135), L132 (≠ P137), D133 (= D138), P212 (≠ D223), W222 (≠ G233)
- binding (5R,7S)-5-(4-ethylphenyl)-7-(trifluoromethyl)-4,5,6,7-tetrahydropyrazolo[1,5-a]pyrimidine-3-carboxylic acid: L77 (= L74), I78 (= I78),