SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_049953503.1 NCBI__GCF_000517625.1:WP_049953503.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 11 hits to proteins with known functional sites (download)

P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
42% identity, 100% coverage: 1:317/318 of query aligns to 1:319/321 of P53571

query
sites
P53571

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M1 (= M1) modified: Initiator methionine, Removed
R211 (= R210) binding FAD
SR 236:237 (= SR 235:236) binding FAD
QVGQS 250:254 (= QVGQS 249:253) binding FAD
268:275 (vs. 266:273, 75% identical) binding FAD
N289 (= N287) binding FAD
DI 307:308 (≠ DL 305:306) binding FAD

3clrD Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
42% identity, 99% coverage: 2:317/318 of query aligns to 1:318/319 of 3clrD

query
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3clrD

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binding flavin-adenine dinucleotide: G209 (= G209), R210 (= R210), G211 (= G211), S235 (= S235), A236 (≠ R236), P237 (= P237), Q249 (= Q249), V250 (= V250), G251 (= G251), Q252 (= Q252), S253 (= S253), G267 (= G266), I268 (= I267), S269 (= S268), S271 (≠ A270), Q273 (= Q272), H274 (= H273), N288 (= N287), T289 (= T288), D306 (= D305), I307 (≠ L306)

6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
33% identity, 99% coverage: 4:317/318 of query aligns to 68:388/393 of 6fahE

query
sites
6fahE

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binding flavin-adenine dinucleotide: L180 (≠ I114), R200 (= R132), M281 (≠ R210), G282 (= G211), R307 (= R236), A308 (≠ P237), Q320 (= Q249), V321 (= V250), G322 (= G251), Q323 (= Q252), T324 (≠ S253), G337 (= G266), I338 (= I267), S339 (= S268), Q343 (= Q272), H344 (= H273), N358 (= N287), K359 (≠ T288), L377 (= L306)

Sites not aligning to the query:

binding iron/sulfur cluster: 7, 10, 13, 17, 18, 35, 36, 37, 38, 41, 45, 49

4kpuA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
32% identity, 99% coverage: 3:318/318 of query aligns to 12:333/338 of 4kpuA

query
sites
4kpuA

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V

D

D

N

E

G

G

W

F

L

I

P

E

K

H

N

P

R

R

Q
|
Q

V
|
V

G
|
G

Q
|
Q

S
|
S

G

G

K

K

V

T

V

V

T

T

P

P

D

K

V

I

Y

Y

I

F

A

A

I

C

G
|
G

I
|
I

S
|
S

G

G

A
x
S

V

V

Q
|
Q

H
|
H

V

K

A

A

G

G

M

M

K

S

G

K

S

S

D

D

T

T

I

I

V

V

A

C

I

I

N
|
N

T
x
K

D

D

E

P

N

D

A

A

P

P

I

M

M

F

D

E

I

I

A

S

D

K

Y

Y

A

G

I

I

Y

V

D

G

D
|
D

L
x
A

F

L

D

K

V

I

V

L

P

P

A

L

L

L

T

T

E

A

Q

K

F

I

R

K

binding flavin-adenine dinucleotide: L125 (≠ I114), R144 (= R132), I155 (≠ V143), G224 (= G209), R225 (= R210), G226 (= G211), S250 (= S235), R251 (= R236), A252 (≠ P237), Q264 (= Q249), V265 (= V250), G266 (= G251), Q267 (= Q252), S268 (= S253), G281 (= G266), I282 (= I267), S283 (= S268), S285 (≠ A270), Q287 (= Q272), H288 (= H273), N302 (= N287), K303 (≠ T288), D320 (= D305), A321 (≠ L306)

P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 6 papers)
34% identity, 99% coverage: 5:318/318 of query aligns to 23:331/333 of P13804

query
sites
P13804

L
|
L

A
x
V

V
x
I

A
|
A

D
x
E

H
|
H

R
x
A

R
x
N

G
x
D

E
x
S

L
|
L

R
x
A

D
x
P

V
x
I

S
x
T

Y
x
L

E
x
N

I
x
T

I
|
I

T
|
T

A
|
A

G
x
A

R
x
T

E
x
R

L
|
L

A

-

D

-

E

-

T

-

G
|
G

E
|
E

L
x
V

H
x
S

L

-

A
x
C

I
x
L

I
x
V

S
x
A

G
|
G

T
|
T

V

-

D

-

F

-

A

-

E

-

K
|
K

A
x
C

N
x
D

R
x
K

E
x
V

G
x
A

V
x
Q

D
|
D

V
x
L

I
x
C

H
x
K

T
x
V

V
x
A

S
x
G

Y
x
I

G
x
A

E
x
K

E
x
V

F
x
L

-
x
V

-
x
A

N
x
Q

H
|
H

D
|
D

V
|
V

Y
|
Y

-
x
K

-
x
G

-
x
L

-
x
P

-
x
E

-
x
L

T
|
T

Q
x
P

A
x
L

I
|
I

T
x
L

Q
x
A

L
x
T

Y
x
Q

D
x
K

E
x
Q

V
x
F

G
x
N

P
x
Y

Q
x
T

Y
x
H

V
x
I

L
x
C

A
|
A

P
x
G

N
x
A

S
|
S

V
x
A

N
x
F

G
|
G

L
x
K

D
x
N

Y
x
L

A
x
L

P
|
P

A
x
R

V
|
V

A
|
A

D
x
A

E
x
K

L
|
L

E
|
E

L
x
V

P
x
A

I
x
P

V
x
I

T
x
S

D
|
D

T
x
I

V
x
I

G
x
A

L
x
I

E
x
K

T
x
S

D
x
P

G

-

E
x
D

T
|
T

L
x
F

I
x
V

A

-

N

-

R
|
R

E
x
T

M
x
I

Y
|
Y

G
x
A

G
|
G

K
x
N

V
x
A

E
x
L

T
x
C

T
|
T

V
|
V

E
x
K

I
x
C

D
|
D

S
x
E

N
x
K

S
x
V

A
x
K

V
|
V

V
x
F

T
x
S

I
x
V

R
|
R

S
x
G

A
x
T

E
x
S

W
x
F

P
x
D

V
x
A

A
|
A

E
x
A

G
x
T

T
x
S

G
|
G

D
x
G

A
x
S

A
|
A

V
x
S

E
x
S

V
x
E

F
x
K

D
x
A

A
x
S

D
x
S

I
x
T

D
x
S

E
x
P

D
x
V

A
x
E

I
|
I

G
x
S

S
x
E

S
x
W

V
x
L

N
x
D

G

-

F

-

E
x
Q

E
x
K

V
x
L

A
x
T

G
x
K

G
x
S

D
|
D

-
x
R

V
x
P

D
x
E

I
x
L

S
x
T

E
x
G

A
|
A

D
x
K

V
|
V

L
x
V

V
|
V

S
|
S

V
x
G

G
|
G

R
|
R

G
|
G

I
x
L

E
x
K

E
x
S

E
x
G

E
|
E

N
|
N

L
x
F

E
x
K

I
x
L

V
x
L

R
x
Y

E
x
D

L
|
L

A
|
A

D
|
D

A
x
Q

L
|
L

D
x
H

A
|
A

T
x
A

L
x
V

S
x
G

S
x
A

S
|
S

R
|
R

P
x
A

I
x
A

V
|
V

D
|
D

N
x
A

G
|
G

W
x
F

L
x
V

P
|
P

K
x
N

N
x
D

R
x
M

Q
|
Q

V
|
V

G
|
G

Q
|
Q

S
x
T

G
|
G

K
|
K

V
x
I

V
|
V

T
x
A

P
|
P

D
x
E

V
x
L

Y
|
Y

I
|
I

A
|
A

I
x
V

G
|
G

I
|
I

S
|
S

G
|
G

A
|
A

V
x
I

Q
|
Q

H
|
H

V
x
L

A
|
A

G
|
G

M
|
M

K
|
K

G
x
D

S
|
S

D
x
K

T
|
T

I
|
I

V
|
V

A
|
A

I
|
I

N
|
N

T
x
K

D
|
D

E
x
P

N
x
E

A
|
A

P
|
P

I
|
I

M
x
F

D
x
Q

I
x
V

A
|
A

D
|
D

Y
|
Y

A
x
G

I
|
I

Y
x
V

D
x
A

D
|
D

L
|
L

F
|
F

D
x
K

V
|
V

P
|
P

A
x
E

L
x
M

T
|
T

E
|
E

Q
x
I

F
x
L

R
x
K

G116 (= G99) to R: in GA2A; impaired protein stability and loss of electron transfer activity; dbSNP:rs119458971
T171 (≠ A157) to I: decreased protein stability; dbSNP:rs1801591
R223 (= R210) binding FAD
S248 (= S235) binding FAD
R249 (= R236) mutation to A: Loss of electron transfer activity.
VGQT 263:266 (≠ VGQS 250:253) binding FAD
T266 (≠ S253) to M: in GA2A; decreased electron transfer activity; dbSNP:rs119458970
SGAIQH 281:286 (≠ SGAVQH 268:273) binding FAD
N300 (= N287) binding FAD
DL 318:319 (= DL 305:306) binding FAD

Sites not aligning to the query:

20:204 Domain I
205:333 Domain II

2a1uA Crystal structure of the human etf e165betaa mutant (see paper)
34% identity, 99% coverage: 5:318/318 of query aligns to 5:313/315 of 2a1uA

query
sites
2a1uA

L

L

A

V

V

I

A

A

D

E

H

H

R

A

R

N

G

D

E

S

L

L

R

A

D

P

V

I

S

T

Y

L

E

N

I

T

I

I

T

T

A

A

G

A

R

T

E

R

L

L

A

-

D

-

E

-

T

-

G

G

E

E

L

V

H

S

L

-

A

C

I

L

I

V

S

A

G

G

T

T

V

-

D

-

F

-

A

-

E

-

K

K

A

C

N

D

R

K

E

V

G

A

V

Q

D

D

V

L

I

C

H

K

T

V

V

A

S

G

Y

I

G

A

E

K

E

V

F

L

-

V

-

A

N

Q

H

H

D

D

V

V

Y

Y

-

K

-

G

-

L

-

P

-

E

-

L

T

T

Q

P

A

L

I

I

T

L

Q

A

L

T

Y

Q

D

K

E

Q

V

F

G

N

P

Y

Q

T

Y

H

V

I

L

C

A

A

P

G

N

A

S

S

V

A

N

F

G

G

L

K

D

N

Y

L

A

L

P

P

A

R

V

V

A

A

D

A

E

K

L

L

E

E

L

V

P

A

I

P

V

I

T

S

D

D

T

I

V

I

G

A

L

I

E

K

T

S

D

P

G

-

E

D

T

T

L

F

I

V

A

-

N

-

R

R

E

T

M

I

Y

Y

G

A

G

G

K

N

V

A

E

L

T

C

T

T

V

V

E

K

I

C

D

D

S

E

N

K

S

V

A

K

V

V

V

F

T

S

I

V

R

R

S

G

A

T

E

S

W

F

P

D

V

A

A

A

E

A

G

T

T

S

G

G

D

G

A

S

A

A

V

S

E

S

V

E

F

K

D

A

A

S

D

S

I

T

D

S

E

P

D

V

A

E

I

I

G

S

S

E

S

W

V

L

N

D

G

-

F

-

E

Q

E

K

V

L

A

T

G

K

G

S

D

D

-

R

V

P

D

E

I

L

S

T

E

G

A

A

D

K

V

V

L

V

V

V

S

S

V

G

G
|
G

R
|
R

G

G

I

L

E

K

E

S

E

G

E

E

N

N

L

F

E

K

I

L

V

L

R

Y

E

D

L

L

A

A

D

D

A

Q

L

L

D

H

A

A

T

A

L

V

S

G

S

A

S
|
S

R
|
R

P
x
A

I

A

V

V

D

D

N

A

G

G

W

F

L

V

P

P

K

N

N

D

R

M

Q
|
Q

V
|
V

G
|
G

Q

Q

S
x
T

G

G

K

K

V

I

V

V

T

A

P

P

D

E

V

L

Y

Y

I

I

A

A

I

V

G
|
G

I
|
I

S
|
S

G

G

A
|
A

V

I

Q
|
Q

H
|
H

V

L

A

A

G

G

M

M

K

K

G

D

S

S

D

K

T

T

I

I

V

V

A

A

I

I

N
|
N

T
x
K

D

D

E

P

N

E

A

A

P

P

I

I

M

F

D

Q

I

V

A

A

D

D

Y

Y

A

G

I

I

Y

V

D

A

D
|
D

L
|
L

F

F

D

K

V

V

P

P

A

E

L

M

T

T

E

E

Q

I

F

L

R

K

binding flavin-adenine dinucleotide: G204 (= G209), R205 (= R210), S230 (= S235), R231 (= R236), A232 (≠ P237), Q244 (= Q249), V245 (= V250), G246 (= G251), T248 (≠ S253), G261 (= G266), I262 (= I267), S263 (= S268), A265 (= A270), Q267 (= Q272), H268 (= H273), N282 (= N287), K283 (≠ T288), D300 (= D305), L301 (= L306)

5ol2A The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
33% identity, 100% coverage: 1:317/318 of query aligns to 1:324/331 of 5ol2A

query
sites
5ol2A

M

M

T

G

D

N

I

V

L

L

A

V

V

V

A

I

D

E

H

Q

R

R

R

E

G

N

E

V

L

I

R

Q

D

T

V

V

S

S

Y

L

E

E

I

L

T

G

A

K

G

A

R

T

E

E

L

I

A

A

D

K

E

D

T

Y

G

D

G

T

E

K

L

V

H

S

L

A

A

L

I

L

S

G

G

S

T

K

V

V

D

E

D

G

F

L

A

I

E

D

K

T

A

L

N

A

R

H

E

Y

G

G

V

A

D

D

V

E

I

V

H

I

T

V

V

V

-

D

S

E

Y

A

G

L

E

A

E

V

F

Y

N

T

H

T

D
x
E

V

P

Y

Y

T

T

Q

K

A

A

I

A

T

Y

Q

E

L

A

Y

I

D

K

E

A

V

A

G

D

P

P

Q

I

Y

V

V

V

L

L

A

F

P

G

N

A

S

T

V

S

N

I

G

G

L

R

D

D

Y

L

A

A

P

P

A

R

V

V

A

S

D

A

E

R

L

I

E

H

L

T

P

G

I

L

V
x
T

T

A

D

D

T

C

V

T

G

G

L

L

E

A

T

V

-

A

-

E

D

D

G

T

E

K

T

L

L

L

I

L

A

M

N

T

R
|
R

E

P

M

A

Y

F

G

G

K

N

V

I

E

M

T

A

T

T

V
x
I

E

V

I

C

-

K

D

D

S

F

N

R

S

P

A

Q

V

M

V

S

T

T

I

V

R

R

S

P

A

G

E

V

W

M

P

K

V

K

A

N

E

E

G

P

-

D

-

E

T

T

G

K

D

E

A

A

A

V

V

I

E

N

V

R

F

F

D

K

A

V

D

E

I

F

-

N

D

D

E

A

D
|
D

A

K

I

L

G

V

S

Q

S

V

V

V

N

Q

G

V

F

I

E

K

E

E

V

-

A

A

G

K

D

Q

V

V

D

K

I

I

S

E

E

D

A

A

D

K

V

I

L

L

V

V

S

S

V

A

G
|
G

R
|
R

G
|
G

I

M

E

G

E

K

E

E

N

N

L

L

E

D

I

I

V

L

R

Y

E

E

L

L

A

A

D

E

A

I

L

I

D

G

A

G

T

E

L

V

S

S

S

G

S
|
S

R
|
R

P
x
A

I

T

V

I

D

D

N

A

G

G

W

W

L

L

P

D

K

K

N

A

R

R

Q
|
Q

V
|
V

G
|
G

Q

Q

S
x
T

G

G

K

K

V

T

V

V

T

R

P

P

D

D

V

L

Y

Y

I

I

A

A

I

C

G
|
G

I
|
I

S
|
S

G

G

A
|
A

V

I

Q
|
Q

H
|
H

V

I

A

A

G

G

M

M

K

E

G

D

S

A

D

E

T

F

I

I

V

V

A

A

I

I

N
|
N

T
x
K

D

N

E

P

N

E

A

A

P

P

I

I

M

F

D

K

I

Y

A

A

D

D

Y

V

A

G

I

I

Y

V

D

G

D
|
D

L
x
V

F

H

D

K

V

V

V

L

P

P

A

E

L

L

T

I

E

S

Q

Q

F

L

binding calcium ion: E75 (≠ D73), D188 (= D180)
binding flavin-adenine dinucleotide: T117 (≠ V115), R136 (= R132), I147 (≠ V143), G216 (= G209), R217 (= R210), G218 (= G211), S242 (= S235), R243 (= R236), A244 (≠ P237), Q256 (= Q249), V257 (= V250), G258 (= G251), T260 (≠ S253), G273 (= G266), I274 (= I267), S275 (= S268), A277 (= A270), Q279 (= Q272), H280 (= H273), N294 (= N287), K295 (≠ T288), D312 (= D305), V313 (≠ L306)

7qh2A Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
31% identity, 97% coverage: 4:313/318 of query aligns to 13:327/337 of 7qh2A

query
sites
7qh2A

I

I

L

T

A

V

V

Y

A

V

D

D

H

H

R

I

R

E

G

G

E

Q

L

I

R

H

D

P

V

V

S

T

Y

F

E

E

I

L

I

I

T

G

A

K

G

A

R

R

E

E

L

L

A

A

D

A

E

V

T

I

G

G

G

H

E

P

L

V

H

Y

L

A

A

L

I

L

I

M

S

G

G

T

T

N

V

I

D

T

D

E

F

K

A

A

E

D

K

E

A

L

N

L

R

K

E

Y

G

G

V

V

D

D

V

K

I

V

H

F

T

-

V

V

S

Y

Y

D

G

K

E

P

E

E

F

L

N

K

H

H

D

F

V

V

-

I

-

E

-

P

Y

Y

T

A

Q

N

A

V

I

L

T

E

Q

D

L

F

Y

I

D

E

E

K

V

V

G

K

P

P

Q

S

Y

S

V

I

L

L

A

V

P

G

N

A

S

T

V

N

N

V

G

G

L

R

D

S

Y

L

A

A

P

P

A

R

V

V

A

A

D

A

E

R

L

Y

E

R

L

T

P

G

I
x
L

V
x
T

T

A

D

D

T

C

V

T

G

I

L

L

E

E

T

M

D

K

G

E

E

N

T

T

-

D

L

L

I

V

A

Q

N

I

R
|
R

E

P

M

A

Y

F

G

G

K

N

V

I

E

M

T

A

T

Q

V
x
I

E

V

I

T

-

E

D

N

S

T

N

R

S

P

A

Q

V

F

V

C

T

T

I

V

R

R

S

Y

A

K

E

V

W

F

P

T

V

A

A

P

E

E

G

R

T

V

G

N

D

E

A

P

A

W

V

G

E

D

V

V

F

E

D

M

A

M

D

D

I

I

D

E

E

K

D

A

A

K

I

L

G

V

S

S

S

A

V

I

N

E

G

V

F

M

E

E

E

V

V

I

A

K

G

K

-

E

G

K

D

G

V

I

D

D

I

L

S

S

E

E

A

A

D

E

V

T

L

I

V

V

S

A

V

V

G

G

R
|
R

G
|
G

I

V

E

K

E

C

E

E

E

K

N

D

L

L

E

D

I

M

V

I

R

H

E

E

L

F

A

A

D

E

A

K

L

I

D

G

A

A

T

T

L

V

S

A

S

C

S
x
T

R
|
R

P

P

I

G

V

I

D

E

N

A

G

G

W

W

L

F

P

D

K

A

N

R

R

L

Q
|
Q

V
x
I

G
|
G

Q
x
L

S
|
S

G

G

K

R

V

T

V

V

T

K

P

P

D

K

V

L

Y

I

I

I

A

A

I

L

G
|
G

I
|
I

S
|
S

G

G

A

A

V

V

Q
|
Q

H

F

V

A

A

A

G

G

M

M

K

Q

G

N

S

S

D

E

T

Y

I

I

V

I

A

A

I

I

N
|
N

T
x
S

D
|
D

E

P

N

K

A

A

P

P

I

I

M

F

D

N

I

I

A

A

D

H

Y

C

A

G

I

M

Y

V

D

G

D
|
D

L
|
L

F

Y

D

E

V

I

V

L

P

P

A

E

L

L

binding flavin-adenine dinucleotide: L125 (≠ I114), T126 (≠ V115), R144 (= R132), I155 (≠ V143), R224 (= R210), G225 (= G211), T249 (≠ S235), R250 (= R236), Q263 (= Q249), I264 (≠ V250), G265 (= G251), L266 (≠ Q252), S267 (= S253), G280 (= G266), I281 (= I267), S282 (= S268), Q286 (= Q272), N301 (= N287), S302 (≠ T288), D303 (= D289), D319 (= D305), L320 (= L306)

1efpA Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
41% identity, 72% coverage: 89:317/318 of query aligns to 84:307/307 of 1efpA

query
sites
1efpA

Q

S

Y

H

V

I

L

A

A

A

P

P

N

A

S

T

V

T

N

D

G

A

L

K

D

N

Y

V

A

M

P

P

A

R

V

V

A

A

D

A

E

L

L

L

E

D

L

V

P

M

I

V

V

L

T

S

D

D

T

V

V

S

G

A

L

I

E

-

T

L

D

D

G

A

E

D

T

T

L

F

I

-

A

-

N

E

R

R

E

P

M

I

Y

Y

G

A

G

G

K

N

V

A

E

I

T

Q

T

V

V

V

E

K

I

S

D

K

S

D

N

A

S

K

A

K

V

V

V

F

T

T

I

I

R

R

S

T

A

A

E

S

W

F

P

D

V

A

A

A

E

G

G

E

T

G

G

G

D

T

A

A

A

P

V

V

E

T

V

E

F

T

D

A

A

A

D

A

I

A

D

D

E

P

D

-

A

-

I

-

G

G

S

L

S

S

V

S

N

W

G

V

F

A

E

D

E

E

V

V

A

A

G

E

G

S

D

D

-

R

V

P

D

E

I

L

S

T

E

S

A

A

D

R

V

R

L

V

V

V

S

S

V

G

G
|
G

R
|
R

G
|
G

I

L

E

G

E

S

E

K

E

E

N

S

L

F

E

A

I

I

V

I

R

E

E

E

L

L

A

A

D

D

A

K

L

L

D

G

A

A

T

A

L

V

S

G

S

A

S
|
S

R
|
R

P
x
A

I

A

V

V

D

D

N

S

G

G

W

Y

L

A

P

P

K

N

N

D

R

W

Q
|
Q

V
|
V

G
|
G

Q

Q

S
x
T

G

G

K

K

V

V

T

A

P

P

D

E

V

L

Y

Y

I

V

A

A

I

V

G
|
G

I
|
I

S
|
S

G

G

A
|
A

V

I

Q
|
Q

H
|
H

V

L

A

A

G

G

M

M

K

K

G

D

S

S

D

K

T

V

I

I

V

V

A

A

I

I

N
|
N

T
x
K

D

D

E

E

N

E

A

A

P

P

I

I

M

F

D

Q

I

I

A

A

D

D

Y

Y

A

G

I

L

Y

V

D

G

D
|
D

L
|
L

F

F

D

S

V

V

P

P

A

E

L

L

T

T

E

G

Q

K

F

L

binding flavin-adenine dinucleotide: G199 (= G209), R200 (= R210), G201 (= G211), S225 (= S235), R226 (= R236), A227 (≠ P237), Q239 (= Q249), V240 (= V250), G241 (= G251), T243 (≠ S253), G256 (= G266), I257 (= I267), S258 (= S268), A260 (= A270), Q262 (= Q272), H263 (= H273), N277 (= N287), K278 (≠ T288), D295 (= D305), L296 (= L306)

7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
34% identity, 99% coverage: 4:318/318 of query aligns to 7:328/336 of 7koeB

query
sites
7koeB

I

I

L

F

A

V

V

L

A

I

D

E

H

H

R

H

R

G

G

G

E

K

L

A

R

H

D

P

V

V

S

S

Y

W

E

E

I

L

I

I

T

G

A

K

G

A

R

R

E

D

L

L

A

A

D

S

E

K

T

L

-

E

G

N

G

S

E

E

L

V

H

W

L

G

A

V

I

L

S

G

G

E

T

G

V

L

D

E

D

S

F

V

A

A

E

K

K

E

A

A

N

I

R

Q

E

R

G

G

V

A

D

D

V

K

I

V

H

L

T

Y

V

V

S

K

Y

-

G

N

E

R

E

E

F

F

N

N

H

T

D

Y

V

V

-

N

-

Y

-

L

Y

Y

T

K

Q

K

A

A

I

L

T

V

Q

D

L

M

Y

V

D

R

E

K

V

Y

G

R

P

P

Q

E

Y

I

V

F

L

L

A

I

P

G

N

A

S

T

V

L

N

E

G

G

L

R

D

E

Y

L

A

A

P

G

A

M

V

V

A

A

D

T

E

E

L

L

E

E

L

T

P

G

I

L

V
x
T

T

A

D

D

T

C

V

T

G

G

L

L

E

D

-

I

T

P

D

D

G

K

E

K

T

L

L

L

I

A

A

M

N

T

R
|
R

E

P

M
x
T

Y

F

G

G

K

N

V

L

E

M

T

A

T

T

V

I

E

M

I

C

-

P

D

D

S

H

N

R

S

P

A

Q

V

M

V

A

T

T

I

V

R

R

S

P

A

G

E

V

W

M

-

K

-

E

-

L

-

P

P

P

V

D

A

P

E

E

G

R

T

T

G

G

D

-

A

-

V

-

E

E

V

I

F

I

D

E

A

E

D

E

I

Y

D

D

E

L

D

G

A

T

I

F

G

D

S

K

S

L

V

I

N

E

G

I

F

L

E

E

E

T

V

I

A

P

-

L

G

Q

G

T

D

Q

V

V

D

N

I

L

S

E

E

Y

A

A

D

P

V

V

L

V

V

V

S

A

V

G

G
|
G

R
x
K

G
|
G

I

V

E

G

E

P

E

E

N

G

L

F

E

K

I

K

V

L

R

K

E

E

L

L

A

A

D

D

A

L

L

L

D

G

A

G

T

E

L

V

S

G

S

A

S
|
S

R
|
R

P
x
A

I

A

V

V

D

K

N

A

G

G

W

W

L

I

P

S

K

P

N

E

R

H

Q
|
Q

V
|
V

G
|
G

Q
|
Q

S
x
T

G

G

K

K

V

T

V

V

T

R

P

P

D

V

V

L

Y

Y

I

F

A

A

I

C

G
|
G

I

I

S
|
S

G

G

A

A

V

I

Q
|
Q

H
|
H

V

V

A

V

G

G

M

I

K

K

G

E

S

S

D

E

T

I

I

I

V

V

A

A

I

I

N
|
N

T
x
I

D

D

E

E

N

K

A

A

P

P

I

I

M

F

D

D

I

I

A

A

D

D

Y

I

A

G

I

I

Y

V

D

G

D

D

L
|
L

F

H

D

K

V

V

P

P

A

A

L

L

T

T

E

A

Q

K

F

L

R

R

binding flavin-adenine dinucleotide: T121 (≠ V115), R140 (= R132), T142 (≠ M134), G219 (= G209), K220 (≠ R210), G221 (= G211), S245 (= S235), R246 (= R236), A247 (≠ P237), Q259 (= Q249), V260 (= V250), G261 (= G251), Q262 (= Q252), T263 (≠ S253), G276 (= G266), S278 (= S268), Q282 (= Q272), H283 (= H273), N297 (= N287), I298 (≠ T288), L316 (= L306)

5ow0A Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
31% identity, 72% coverage: 89:318/318 of query aligns to 70:292/292 of 5ow0A

query
sites
5ow0A

Q

E

Y

L

V

I

L

L

A

L

P

S

N

T

S

T

V

A

N

I

G

G

L

S

D

G

Y

L

A

A

P

G

A

P

V

L

A

A

D

V

E

S

L

L

E

G

L

A

P

P

I

I

V

L

T

S

D

E

T

V

V

T

G

A

L

I

E

S

T

P

D

D

G

-

E

-

T

-

L

L

I

T

A

I

N

E

R

R

E

S

M

L

Y

Y

G

G

G

S

K

K

V

A

E

V

T

A

T

R

V

Y

E

K

I

L

D

E

S

S

N

G

S

P

A

L

V

V

V

L

T

T

I

I

R

K

S

R

A

K

E

Y

W

F

P

E

V

A

A

A

E

T

G

L

T

E

G

G

D

T

A

T

A

A

V

T

E

E

V

E

F

L

D

P

A

V

D

G

I

P

D

Q

E

K

D

I

A

T

I

L

G

L

S

E

V

I

N

E

G

E

F

E

E

R

E

T

V

-

A

-

G

-

D

G

V

I

D

P

I

L

S

E

E

D

A

A

D

E

V

V

L

V

V

V

S

T

V

G

G
|
G

R
|
R

G
|
G

I

I

E

G

E

S

E

G

E

D

N

N

L

F

E

S

I

I

V

L

R

K

E

E

L

I

A

A

D

G

A

M

L

L

D

N

A

G

T

A

L

V

S

G

S

A

S
|
S

R
|
R

P

G

I

A

V

V

D

D

N

E

G

G

W

W

L

M

P

P

K

P

N

G

R

A

Q
|
Q

V
x
I

G
|
G

Q

Q

S
x
T

G

G

K

K

V

I

V

V

T

A

P

P

D

T

V

V

Y

Y

I

F

A

A

I

V

G
|
G

I
x
V

S
|
S

G

G

A
|
A

V

S

Q
|
Q

H
|
H

V

L

A

A

G

G

M

I

K

S

G

N

S

A

D

K

T

C

I

V

V

I

A

A

I

I

N
|
N

T
x
K

D

D

E

N

N

E

A

A

P

N

I

I

M

F

D

K

I

R

A

A

D

R

Y

F

A

G

I

I

Y

V

D

G

D
|
D

L
x
Y

F

K

D

K

V

A

V

V

P

P

A

A

L

L

T

I

E

N

Q

A

F

L

R

K

binding flavin-adenine dinucleotide: G183 (= G209), R184 (= R210), G185 (= G211), S209 (= S235), R210 (= R236), Q223 (= Q249), I224 (≠ V250), G225 (= G251), T227 (≠ S253), G240 (= G266), V241 (≠ I267), S242 (= S268), A244 (= A270), Q246 (= Q272), H247 (= H273), N261 (= N287), K262 (≠ T288), D279 (= D305), Y280 (≠ L306)

Query Sequence

>WP_049953503.1 NCBI__GCF_000517625.1:WP_049953503.1
MTDILAVADHRRGELRDVSYEIITAGRELADETGGELHLAIISGTVDDFAEKANREGVDV
IHTVSYGEEFNHDVYTQAITQLYDEVGPQYVLAPNSVNGLDYAPAVADELELPIVTDTVG
LETDGETLIANREMYGGKVETTVEIDSNSAVVTIRSAEWPVAEGTGDAAVEVFDADIDED
AIGSSVNGFEEVAGGDVDISEADVLVSVGRGIEEEENLEIVRELADALDATLSSSRPIVD
NGWLPKNRQVGQSGKVVTPDVYIAIGISGAVQHVAGMKGSDTIVAINTDENAPIMDIADY
AIYDDLFDVVPALTEQFR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory