SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_049989922.1 NCBI__GCF_000784335.1:WP_049989922.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

7cagA Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
35% identity, 88% coverage: 34:309/314 of query aligns to 6:278/285 of 7cagA

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7cagA

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binding alpha-D-glucopyranose: E159 (≠ D188), K162 (≠ Q191), R216 (= R245), D219 (= D248)

P94529 Arabinooligosaccharides transport system permease protein AraP from Bacillus subtilis (strain 168) (see paper)
29% identity, 92% coverage: 4:292/314 of query aligns to 5:288/313 of P94529

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E205 (≠ H210) mutation to A: Has no effect on the growth rate.
E208 (= E213) mutation to A: Small negative impact in the uptake of arabinotriose. Shows an increase of about 10% in the doubling time.
D213 (= D218) mutation to A: Severely impairs the uptake of arabinotriose. Shows a 3.6-fold increase of the doubling time.

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
23% identity, 61% coverage: 122:314/314 of query aligns to 309:513/514 of P02916

query
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P02916

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L334 (= L147) mutation to W: Ability to transport lactose in a saturable manner.
L372 (≠ V184) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ D188) mutation N->K,H: No growth on maltose.
G380 (≠ W192) mutation G->C,S: No growth on maltose.
E401 (= E213) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A215) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G219) mutation G->A,P: No effect.
P420 (= P232) mutation to A: No effect.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
24% identity, 58% coverage: 122:304/314 of query aligns to 294:488/490 of 4ki0F

query
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4ki0F

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binding alpha-D-glucopyranose: Y310 (≠ M138), N361 (≠ D188), G365 (≠ W192), Y368 (≠ L195), S418 (≠ R245), N422 (≠ A249)

Query Sequence

>WP_049989922.1 NCBI__GCF_000784335.1:WP_049989922.1
MATETGTDVRPTADLEEQLGAEQSLRERTLLWIDERLKWLMTLPAVFLLFALTFYPLVRA
VQMSTQRYQPGGRTVFVGVENYINLLNNGAFLQSLWVTGKFIGLAVTIEFLLGFAIAIAL
NKKIKLRGLWQTMILVPMILSPTVIGLIWRLMYTPDGLLDYMAMPLVGGNVGWISDPGVA
LYAVVLTDVWQWTPLVVLVIFAGLQSVPSHIQEAAVMDGASRWRRFVDIVFPYLKSLIVL
VLIIRVVDALRVFAKVYILTRGGPSNATNVISMEMYRTAFRFNNFGEASAMAISLLAVVL
VLAMSFVKIANIEF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory