SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_050463021.1 NCBI__GCF_001189915.1:WP_050463021.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8i01A Crystal structure of escherichia coli glyoxylate carboligase (see paper)
77% identity, 100% coverage: 1:590/591 of query aligns to 2:591/594 of 8i01A

query
sites
8i01A

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binding flavin-adenine dinucleotide: R155 (= R154), G212 (= G211), G213 (= G212), G214 (= G213), N217 (= N216), T238 (= T237), L239 (= L238), M240 (= M239), V256 (= V255), G257 (= G256), Q259 (= Q258), T260 (= T259), G279 (= G278), N280 (= N279), R281 (= R280), A283 (= A282), R285 (= R284), H286 (= H285), D303 (= D302), I304 (= I303), Q308 (= Q307), D322 (= D321), A323 (= A322), G417 (= G416)
binding magnesium ion: D447 (= D446), F452 (= F451), E455 (= E454), N474 (= N473), Y476 (= Y475)
binding thiamine diphosphate: G395 (= G394), L396 (= L395), S397 (= S396), L422 (= L421), G446 (= G445), D447 (= D446), F448 (≠ Y447), D449 (= D448), N474 (= N473), Y476 (= Y475), L477 (= L476), G478 (= G477), L479 (= L478), I480 (= I479)
binding 2,3-dimethoxy-5-methyl-1,4-benzoquinone: Q354 (≠ R353), C492 (= C491), Q494 (= Q493), C589 (≠ S588)

8beoB Crystal structure of e. Coli glyoxylate carboligase mutant i393a with map
77% identity, 100% coverage: 2:590/591 of query aligns to 1:589/592 of 8beoB

query
sites
8beoB

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binding methyl hydrogen (s)-acetylphosphonate: C490 (= C491), Q492 (= Q493)
binding flavin-adenine dinucleotide: R153 (= R154), P154 (= P155), G210 (= G211), G211 (= G212), G212 (= G213), N215 (= N216), T236 (= T237), L237 (= L238), M238 (= M239), V254 (= V255), G255 (= G256), Q257 (= Q258), T258 (= T259), G277 (= G278), N278 (= N279), R279 (= R280), A281 (= A282), R283 (= R284), H284 (= H285), D301 (= D302), I302 (= I303), Q306 (= Q307), D320 (= D321), A321 (= A322), G415 (= G416)
binding magnesium ion: D445 (= D446), F450 (= F451), L451 (≠ M452), E453 (= E454), N472 (= N473), Y474 (= Y475)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(1s)-1-hydroxy-1-[(r)-hydroxy(methoxy)phosphoryl]ethyl}-5-(2-{[(s)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: R283 (= R284), G393 (= G394), L394 (= L395), S395 (= S396), L420 (= L421), G444 (= G445), D445 (= D446), F446 (≠ Y447), D447 (= D448), N472 (= N473), Y474 (= Y475), L475 (= L476), G476 (= G477), L477 (= L478), I478 (= I479)
binding 2,3-dimethoxy-5-methyl-1,4-benzoquinone: Q352 (≠ R353), R356 (= R357), C587 (≠ S588)

8beoA Crystal structure of e. Coli glyoxylate carboligase mutant i393a with map
77% identity, 100% coverage: 2:590/591 of query aligns to 1:589/592 of 8beoA

query
sites
8beoA

A

A

K

K

M

M

K

R

A

A

V

V

D

D

A

A

A

A

V

M

Y

Y

V

V

L

L

E

E

K

K

E

E

G

G

C

I

L

T

Q

T

A

A

F

F

G

G

V

V

P

P

G

G

A

A

A

A

I

I

N

N

P

P

F

F

Y

Y

A

S

A

A

L

M

K

R

R

K

H

H

G

G

G

G

V

I

K

R

H

H

V

I

L

L

A

A

R

R

H

H

V

V

E

E

G

G

A

A

S

S

H

H

M

M

A

A

E

E

G

G

Y

Y

T

T

R

R

A

A

N

T

P

A

G

G

N

N

I

I

G

G

L

V

C

C

I

L

G

G

T

T

S

S

G

G

P

P

A

A

G

G

T

T

D

D

M

M

I

I

T

T

G

A

L

L

Y

Y

S

S

A

A

Q

S

A

A

D

D

S

S

I

I

P

P

I

I

L

L

C

C

V

I

T

T

G

G

Q

Q

A

A

P

P

R

R

A

A

R

R

L

L

Y

H

K

K

E

E

D

D

F

F

Q

Q

A

A

V

V

D

D

I

I

E

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I

I

A

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K

P

P

V

V

T

S

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K

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M

A

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V

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T

V

V

R

R

E

E

P

A

A

A

L

L

V

V

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P

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V

F

L

Q

Q

Q

Q

A

A

F

F

H

H

L

L

M

M

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R

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S

G

G

R
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R

P
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P

G

G

P

P

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I

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D

M

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P

P

F

F

D

D

V

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M

V

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A

E

E

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I

E

E

F

F

D

D

P

P

D

D

T

M

Y

Y

E

E

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L

P

P

V

V

Y

Y

R

K

P

P

S

A

A

A

S

S

R

R

A

M

Q

Q

I

I

E

E

K

K

A

A

L

V

S

E

M

M

L

L

N

I

A

Q

A

A

E

E

R

R

P

P

L

V

L

I

T

V

I

A

G
|
G

G
|
G

G
|
G

V

V

I

I

N
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N

A

A

D

D

A

A

S

A

A

A

L

L

A

L

V

Q

E

Q

F

F

A

A

E

E

L

L

T

T

G

S

V

V

P

P

V

V

I

I

P

P

T
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T

L
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L

M
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M

A

G

W

W

G

G

A

C

I

I

P

P

D

D

D

D

H

H

P
x
E

Q

L

M

M

A

A

G

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M

M

V
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V

G
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G

L

L

Q
|
Q

T
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T

S

A

H

H

R

R

Y

Y

G

G

N

N

A

A

S

T

M

L

L

L

A

A

S

S

D

D

F

M

V

V

L

F

G

G

I

I

G
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G

N
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N

R
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R

W

F

A
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A

N

N

R
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R

H
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H

T

T

G

G

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D

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K

F

Y

T

T

K

E

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R

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I

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I

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D

I
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I

E

E

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I

I

G

G

R

R

V

V

F

L

G

C

P

P

D

D

Y

L

G

G

I

I

V

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S

S

D
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D

A
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A

K

K

A

A

A

A

L

L

T

T

L

L

F

L

I

V

E

E

V

V

A

A

K

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E

E

W

M

Q

Q

A

K

A

A

G

G

K

R

L

L

P

P

D

C

R

R

S

K

A

E

W

W

L

V

A

A

E

D

C

C

R
x
Q

E

Q

R

R

K

K

R

R

T

T

L

L

Q

L

R

R

R

K

T

T

N

H

F

F

T

D

E

N

V

V

P

P

I

V

K

K

P

P

Q

Q

R

R

V

V

Y

Y

E

E

E

E

M

M

N

N

K

K

A

A

F

F

D

G

R
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R

N

D

T

V

C

C

Y

Y

V

V

S

T

T

T

I

A

G
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G

L
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L

S
|
S

Q

Q

I
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I

A

A

A

A

A

A

Q

Q

F

M

L

L

H

H

V
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V

Y
x
F

K

K

P

D

R

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H
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H

W

W

I

I

N

N

C

C

G
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G

Q

Q

A

A

G

G

P

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L

G

G

W

W

T

T

I

I

P

P

A

A

A

A

L

L

G

G

V

V

C

C

A

A

A

A

D

D

P

P

Q

K

R

R

K

N

V

V

V

V

A

A

I

I

S

S

G
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G

D
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D

Y
x
F

D
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D

F

F

Q

Q

F
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F

M
x
L

I

I

E
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E

E

E

L

L

A

A

V

V

G

G

A

A

Q

Q

F

F

N

N

L

I

P

P

Y

Y

L

I

H

H

V

V

V

L

V

V

N

N

N
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N

S

A

Y
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Y

L
|
L

G
|
G

L
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L

I
|
I

R

R

Q

Q

A

S

Q

Q

R

R

G

A

F

F

E

D

M

M

D

D

Y

Y

C

C

V

V

Q

Q

L

L

A

A

F

F

E

E

N

N

I

I

N

N

A

S

P

S

E

E

L

V

N

N

G

G

Y

Y

G

G

V

V

D

D

H

H

V

V

A

K

V

V

V

A

E

E

G

G

L

L

G

G

C

C

K

K

A

A

I

I

R

R

V

V

R

F

D

K

P

P

N

E

A

D

I

I

Q

A

D

P

A

A

F

F

A

E

Q

Q

A

A

K

K

R

A

W

L

M

M

E

A

E

Q

Y

Y

R

R

V

V

P

P

V

V

V

V

V

V

E

E

I

V

I

I

L

L

E

E

R

R

V

V

T

T

N

N

I

I

S

S

M

M

G

G

T

S

E

E

I

L

D

D

A

N

V

V

N

M

E

E

F

F

E

E

E

D

L

I

A

A

T

D

R

N

R

A

E

A

H

D

A

A

P

P

T

T

A

E

V

T

S
x
C

L

F

L

M

binding flavin-adenine dinucleotide: R153 (= R154), P154 (= P155), G210 (= G211), G211 (= G212), G212 (= G213), N215 (= N216), T236 (= T237), L237 (= L238), M238 (= M239), V254 (= V255), G255 (= G256), Q257 (= Q258), T258 (= T259), G277 (= G278), N278 (= N279), R279 (= R280), A281 (= A282), R283 (= R284), H284 (= H285), D301 (= D302), I302 (= I303), Q306 (= Q307), D320 (= D321), A321 (= A322), I397 (= I398), G415 (= G416)
binding magnesium ion: R384 (= R385), V405 (= V406), F406 (≠ Y407), H410 (= H411), D445 (= D446), F450 (= F451), L451 (≠ M452), E453 (= E454), N472 (= N473), Y474 (= Y475)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(1s)-1-hydroxy-1-[(r)-hydroxy(methoxy)phosphoryl]ethyl}-5-(2-{[(s)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: R283 (= R284), G393 (= G394), L394 (= L395), S395 (= S396), L420 (= L421), G444 (= G445), D445 (= D446), F446 (≠ Y447), D447 (= D448), N472 (= N473), Y474 (= Y475), L475 (= L476), G476 (= G477), L477 (= L478), I478 (= I479)
binding 2,3-dimethoxy-5-methyl-1,4-benzoquinone: E248 (≠ P249), Q352 (≠ R353), C587 (≠ S588)

8i07D Crystal structure of escherichia coli glyoxylate carboligase double mutant in complex with glycolaldehyde (see paper)
77% identity, 100% coverage: 1:590/591 of query aligns to 2:591/594 of 8i07D

query
sites
8i07D

M

M

A

A

K

K

M

M

K

R

A

A

V

V

D

D

A

A

A

A

V

M

Y

Y

V

V

L

L

E

E

K

K

E

E

G

G

C

I

L

T

Q

T

A

A

F

F

G

G

V

V

P

P

G

G

A

A

A

A

I

I

N

N

P

P

F

F

Y

Y

A

S

A

A

L

M

K

R

R

K

H

H

G

G

G

G

V

I

K

R

H

H

V

I

L

L

A

A

R

R

H

H

V
|
V

E

E

G

G

A

A

S

S

H

H

M

M

A

A

E

E

G

G

Y

Y

T

T

R

R

A

A

N

T

P

A

G

G

N

N

I

I

G

G

L

V

C

C

I

L

G

G

T
|
T

S

S

G

G

P

P

A

A

G

G

T

T

D

D

M

M

I

I

T

T

G

A

L

L

Y

Y

S

S

A

A

Q

S

A

A

D

D

S

S

I

I

P

P

I

I

L

L

C

C

V

I

T

T

G

G

Q

Q

A

A

P

P

R

R

A

A

R

R

L

L

Y

H

K

K

E

E

D

D

F

F

Q

Q

A

A

V

V

D

D

I

I

E

E

S

A

I

I

A

A

K

K

P

P

V

V

T

S

K

K

W

M

A

A

V

V

T

T

V

V

R

R

E

E

P

A

A

A

L

L

V

V

P

P

R

R

V

V

F

L

Q

Q

Q

Q

A

A

F

F

H

H

L

L

M

M

R

R

S

S

G

G

R
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R

P
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P

G

G

P

P

V

V

L

L

I

V

D

D

M

L

P

P

F

F

D

D

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M

V

A

A

E

E

I

I

E

E

F

F

D

D

P

P

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D

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M

Y

Y

E

E

P

P

L

L

P

P

V

V

Y

Y

R

K

P

P

S

A

A

A

S

S

R

R

A

M

Q

Q

I

I

E

E

K

K

A

A

L

V

S

E

M

M

L

L

N

I

A

Q

A

A

E

E

R

R

P

P

L

V

L

I

T

V

I

A

G
|
G

G
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G

G
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G

V

V

I

I

N
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N

A

A

D

D

A

A

S

A

A

A

L

L

A

L

V

Q

E

Q

F

F

A

A

E

E

L

L

T

T

G

S

V

V

P

P

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V

I

I

P

P

T
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T

L
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L

M
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M

A

G

W

W

G

G

A

C

I

I

P

P

D

D

D

D

H

H

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E

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M

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A

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M

V
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V

G
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G

L

L

Q
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Q

T
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T

S

A

H

H

R

R

Y

Y

G

G

N

N

A

A

S

T

M

L

L

L

A

A

S

S

D

D

F

M

V

V

L

F

G

G

I

I

G
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G

N
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N

R
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R

W

F

A

A

N

Q

R
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R

H
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H

T

T

G

G

S

S

V

V

D

E

V

K

F

Y

T

T

K

E

G

G

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K

K

F

I

V

V

H

H

V

I

D
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D

I
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I

E

E

P

P

T

T

Q
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Q

I

I

G

G

R

R

V

V

F

L

G

C

P

P

D

D

Y

L

G

G

I

I

V

V

S

S

D
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D

A
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A

K

K

A

A

A

A

L

L

T

T

L

L

F

L

I

V

E

E

V

V

A

A

K

Q

E

E

W

M

Q

Q

A

K

A

A

G

G

K

R

L

L

P

P

D

C

R

R

S

K

A

E

W

W

L

V

A

A

E

D

C

C

R
x
Q

E

Q

R

R

K

K

R
|
R

T

T

L

L

Q

L

R

R

R

K

T

T

N

H

F

F

T

D

E

N

V

V

P

P

I

V

K

K

P

P

Q

Q

R

R

V

V

Y

Y

E

E

E

E

M

M

N

N

K

K

A

A

F

F

D

G

R

R

N

D

T

V

C

C

Y

Y

V

V

S

T

T

T

I

I

G
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G

L
|
L

S
|
S

Q

Q

I
|
I

A

A

A

A

A

A

Q

Q

F

M

L

L

H

H

V

V

Y

F

K

K

P

D

R

R

H

H

W

W

I

I

N

N

C

C

G
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G

Q

Q

A

A

G

G

P

P

L
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L

G

G

W

W

T

T

I

I

P

P

A

A

A

A

L

L

G

G

V

V

C

C

A

A

A

A

D

D

P

P

Q

K

R

R

K

N

V

V

V

V

A

A

I

I

S

S

G
|
G

D
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D

Y
x
F

D
|
D

F

F

Q

Q

F
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F

M
x
L

I

I

E
|
E

E

E

L

L

A

A

V

V

G

G

A

A

Q

Q

F

F

N

N

L

I

P

P

Y

Y

L

I

H

H

V

V

V

L

V

V

N

N

N
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N

S

A

Y
|
Y

L
|
L

G
|
G

L
|
L

I
|
I

R

R

Q

Q

A

S

Q

Q

R

M

G

A

F

F

E

D

M

M

D

D

Y

Y

C
|
C

V

V

Q
|
Q

L

L

A

A

F

F

E

E

N

N

I

I

N

N

A

S

P

S

E

E

L

V

N

N

G

G

Y

Y

G

G

V

V

D

D

H

H

V

V

A

K

V

V

V

A

E

E

G

G

L

L

G

G

C

C

K

K

A

A

I

I

R

R

V

V

R

F

D

K

P

P

N

E

A

D

I

I

Q

A

D

P

A

A

F

F

A

E

Q

Q

A

A

K

K

R

A

W

L

M

M

E

A

E

Q

Y

Y

R

R

V

V

P

P

V

V

V

V

V

V

E

E

I

V

I

I

L

L

E

E

R

R

V

V

T

T

N

N

I

I

S

S

M

M

G

G

T

S

E

E

I

L

D

D

A

N

V

V

N

M

E

E

F

F

E

E

E

D

L

I

A

A

T

D

R

N

R

A

E

A

H

D

A

A

P

P

T

T

A

E

V

T

S
x
C

L

F

L

M

binding 2-oxidanylethanal: R285 (= R284), I480 (= I479)
binding flavin-adenine dinucleotide: R155 (= R154), P156 (= P155), G212 (= G211), G213 (= G212), G214 (= G213), N217 (= N216), T238 (= T237), L239 (= L238), M240 (= M239), V256 (= V255), G257 (= G256), Q259 (= Q258), T260 (= T259), G279 (= G278), N280 (= N279), R281 (= R280), R285 (= R284), H286 (= H285), D303 (= D302), I304 (= I303), Q308 (= Q307), D322 (= D321), A323 (= A322), I399 (= I398), G417 (= G416)
binding magnesium ion: D447 (= D446), F452 (= F451), L453 (≠ M452), E455 (= E454), N474 (= N473), Y476 (= Y475)
binding thiamine diphosphate: V52 (= V51), T76 (= T75), G395 (= G394), L396 (= L395), S397 (= S396), L422 (= L421), G446 (= G445), D447 (= D446), F448 (≠ Y447), D449 (= D448), N474 (= N473), Y476 (= Y475), L477 (= L476), G478 (= G477), L479 (= L478), I480 (= I479)
binding ubiquinone-1: Q354 (≠ R353), R358 (= R357), C492 (= C491), Q494 (= Q493), C589 (≠ S588)

8i07A Crystal structure of escherichia coli glyoxylate carboligase double mutant in complex with glycolaldehyde (see paper)
77% identity, 100% coverage: 1:590/591 of query aligns to 2:591/594 of 8i07A

query
sites
8i07A

M

M

A

A

K

K

M

M

K

R

A

A

V

V

D

D

A

A

A

A

V

M

Y

Y

V

V

L

L

E

E

K

K

E

E

G

G

C

I

L

T

Q

T

A

A

F

F

G

G

V

V

P

P

G

G

A

A

A

A

I

I

N

N

P

P

F

F

Y

Y

A

S

A

A

L

M

K

R

R

K

H

H

G

G

G

G

V

I

K

R

H

H

V

I

L

L

A

A

R

R

H

H

V

V

E

E

G

G

A

A

S

S

H

H

M

M

A

A

E

E

G

G

Y

Y

T

T

R

R

A

A

N

T

P

A

G

G

N

N

I

I

G

G

L

V

C

C

I

L

G

G

T

T

S

S

G

G

P

P

A

A

G

G

T

T

D

D

M

M

I

I

T

T

G

A

L

L

Y

Y

S

S

A

A

Q

S

A

A

D

D

S

S

I

I

P

P

I

I

L

L

C

C

V

I

T

T

G

G

Q

Q

A

A

P

P

R

R

A

A

R

R

L

L

Y

H

K

K

E

E

D

D

F

F

Q

Q

A

A

V

V

D

D

I

I

E

E

S

A

I

I

A

A

K

K

P

P

V

V

T

S

K

K

W

M

A

A

V

V

T

T

V

V

R

R

E

E

P

A

A

A

L

L

V

V

P

P

R

R

V

V

F

L

Q

Q

Q

Q

A

A

F

F

H

H

L

L

M

M

R

R

S

S

G

G

R
|
R

P
|
P

G

G

P

P

V

V

L

L

I

V

D

D

M

L

P

P

F

F

D

D

V

V

Q

Q

M

V

A

A

E

E

I

I

E

E

F

F

D

D

P

P

D

D

T

M

Y

Y

E

E

P

P

L

L

P

P

V

V

Y

Y

R

K

P

P

S

A

A

A

S

S

R

R

A

M

Q

Q

I

I

E

E

K

K

A

A

L

V

S

E

M

M

L

L

N

I

A

Q

A

A

E

E

R

R

P

P

L

V

L

I

T

V

I

A

G
|
G

G
|
G

G
|
G

V

V

I

I

N
|
N

A

A

D

D

A

A

S

A

A

A

L

L

A

L

V

Q

E

Q

F

F

A

A

E

E

L

L

T

T

G

S

V

V

P

P

V

V

I

I

P

P

T
|
T

L
|
L

M
|
M

A

G

W

W

G

G

A

C

I

I

P

P

D

D

D

D

H

H

P

E

Q

L

M

M

A

A

G

G

M

M

V
|
V

G
|
G

L

L

Q
|
Q

T
|
T

S

A

H

H

R

R

Y

Y

G

G

N

N

A

A

S

T

M

L

L

L

A

A

S

S

D

D

F

M

V

V

L

F

G

G

I

I

G
|
G

N
|
N

R
|
R

W

F

A

A

N

Q

R
|
R

H
|
H

T

T

G

G

S

S

V

V

D

E

V

K

F

Y

T

T

K

E

G

G

R

R

K

K

F

I

V

V

H

H

V

I

D
|
D

I
|
I

E

E

P

P

T

T

Q
|
Q

I

I

G

G

R

R

V

V

F

L

G

C

P

P

D

D

Y

L

G

G

I

I

V

V

S

S

D
|
D

A
|
A

K

K

A

A

A

A

L

L

T

T

L

L

F

L

I

V

E

E

V

V

A

A

K

Q

E

E

W

M

Q

Q

A

K

A

A

G

G

K

R

L

L

P

P

D

C

R

R

S

K

A

E

W

W

L

V

A

A

E

D

C

C

R
x
Q

E

Q

R

R

K

K

R

R

T

T

L

L

Q

L

R

R

R

K

T

T

N

H

F

F

T

D

E

N

V

V

P

P

I

V

K

K

P

P

Q

Q

R

R

V

V

Y

Y

E

E

E

E

M

M

N

N

K

K

A

A

F

F

D

G

R

R

N

D

T

V

C

C

Y

Y

V

V

S

T

T

T

I
|
I

G
|
G

L
|
L

S
|
S

Q

Q

I
|
I

A

A

A

A

A

A

Q

Q

F

M

L

L

H

H

V

V

Y

F

K

K

P

D

R

R

H

H

W

W

I

I

N

N

C

C

G
|
G

Q

Q

A

A

G

G

P

P

L
|
L

G

G

W

W

T

T

I

I

P

P

A

A

A

A

L

L

G

G

V

V

C

C

A

A

A

A

D

D

P

P

Q

K

R

R

K

N

V

V

V

V

A

A

I

I

S

S

G
|
G

D
|
D

Y
x
F

D
|
D

F

F

Q

Q

F
|
F

M
x
L

I

I

E
|
E

E

E

L

L

A

A

V

V

G

G

A

A

Q

Q

F

F

N

N

L

I

P

P

Y

Y

L

I

H

H

V

V

V

L

V

V

N

N

N
|
N

S

A

Y
|
Y

L
|
L

G
|
G

L
|
L

I
|
I

R

R

Q

Q

A

S

Q

Q

R

M

G

A

F

F

E

D

M

M

D

D

Y

Y

C
|
C

V

V

Q
|
Q

L

L

A

A

F

F

E

E

N

N

I

I

N

N

A

S

P

S

E

E

L

V

N

N

G

G

Y

Y

G

G

V

V

D

D

H

H

V

V

A

K

V

V

V

A

E

E

G

G

L

L

G

G

C

C

K

K

A

A

I

I

R

R

V

V

R

F

D

K

P

P

N

E

A

D

I

I

Q

A

D

P

A

A

F

F

A

E

Q

Q

A

A

K

K

R

A

W

L

M

M

E

A

E

Q

Y

Y

R

R

V

V

P

P

V

V

V

V

V

V

E

E

I

V

I

I

L

L

E

E

R

R

V

V

T

T

N

N

I

I

S

S

M

M

G

G

T

S

E

E

I

L

D

D

A

N

V

V

N

M

E

E

F

F

E

E

E

D

L

I

A

A

T

D

R

N

R

A

E

A

H

D

A

A

P

P

T

T

A

E

V

T

S
x
C

L

F

L

M

binding flavin-adenine dinucleotide: R155 (= R154), P156 (= P155), G212 (= G211), G213 (= G212), G214 (= G213), N217 (= N216), T238 (= T237), L239 (= L238), M240 (= M239), V256 (= V255), G257 (= G256), Q259 (= Q258), T260 (= T259), G279 (= G278), N280 (= N279), R281 (= R280), R285 (= R284), H286 (= H285), D303 (= D302), I304 (= I303), Q308 (= Q307), D322 (= D321), A323 (= A322), I394 (= I393), I399 (= I398), G417 (= G416)
binding magnesium ion: D447 (= D446), F452 (= F451), L453 (≠ M452), E455 (= E454), N474 (= N473), Y476 (= Y475)
binding thiamine diphosphate: I394 (= I393), G395 (= G394), L396 (= L395), S397 (= S396), L422 (= L421), G446 (= G445), D447 (= D446), F448 (≠ Y447), D449 (= D448), N474 (= N473), Y476 (= Y475), L477 (= L476), G478 (= G477), L479 (= L478), I480 (= I479)
binding ubiquinone-1: Q354 (≠ R353), C492 (= C491), Q494 (= Q493), C589 (≠ S588)

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
35% identity, 82% coverage: 5:486/591 of query aligns to 95:575/667 of P09342

query
sites
P09342

K

K

A

G

V

S

D

D

A

V

A

L

V

V

Y

E

V

A

L

L

E

E

K

R

E

E

G

G

C

V

L

T

Q

D

A

V

F

F

G

A

V

Y

P

P

G

G

A

G

A

A

I

S

N

M

P

E

F

I

Y

H

A

Q

A

A

L

L

K

T

R

R

H

S

G

S

G

I

V

I

K

R

H

N

V

V

L

L

A

P

R

R

H

H

V

E

E

Q

G

G

A

G

S

V

H

F

M

A

A

A

E

E

G

G

Y

Y

T

A

R

R

A

A

N

T

P

-

G

G

N

F

I

P

G

G

L

V

C
|
C

I

I

G

A

T

T

S

S

G

G

P

P

A

G

G

A

T

T

D

N

M

L

I

V

T

S

G

G

L

L

Y

A

S

D

A

A

Q

L

A

L

D

D

S

S

I

V

P

P

I

I

L

V

C

A

V

I

T

T

G

G

Q

Q

A

V

P
|
P

R

R

A

R

R

M

L

I

Y

G

K

T

E

D

D

A

F

F

Q

Q

A

E

V

T

D

P

I

I

E

V

S

E

I

V

A

T

K

R

P

S

V

I

T

T

K

K

W

H

A

N

V

Y

T

L

V

V

R

M

E

D

P

V

A

E

L

D

V

I

P

P

R

R

V

V

F

V

Q

R

Q

E

A

A

F

F

H

F

L

L

M

A

R

R

S

S

G

G

R

R

P

P

G

G

P

P

V

I

L

L

I

I

D

D

M

V

P

P

F

K

D

D

V

I

Q

Q

M

Q

A

Q

E

L

I

V

E

I

F

P

D

D

P

W

D

D

T

Q

Y

P

E

M

P

R

L

L

P

P

V

G

Y

Y

R

M

P

S

S

R

A

L

S

P

R

K

A

L

Q

P

-

N

-

E

-

M

-

L

I

L

E

E

K

Q

A

I

L

V

S

R

M

L

L

I

N

S

A

E

A

S

E

K

R

K

P

P

L

V

L

L

T

Y

I

V

G

G

G

G

G

G

V
x
C

I

-

N

-

A

S

D

Q

A

S

S

S

A

E

L

D

A

L

V

R

E

R

F

F

A

V

E

E

L

L

T

T

G

G

V

I

P

P

V

V

I

A

P

S

T

T

L

L

M

M

A

G

W

L

G

G

A

A

I

F

P

P

D

T

D

G

H

D

P

E

Q

L

M

S

A

L

G

S

M

M

V

L

G

G

L

M

Q

H

T

G

S

T

H

-

R

V

Y

Y

G

A

N

N

A

Y

S

A

M

V

L

D

A

S

S

S

D

D

F

L

V

L

L

L

G

A

I

F

G

G

N

V

R

R

W

F

A

D

N

D

R

R

H

V

T

T

G

G

S

K

V

L

D

E

V

A

F

F

T

A

K

S

G

R

R

A

K

K

F

I

V

V

H

H

V

I

D

D

I

I

E

D

P

S

T

A

Q

E

I

I

G

G

R

K

V

N

F

K

G

Q

P

P

D

H

Y

V

G

S

I

I

V

C

S

A

D

D

A

I

K

K

A

L

A

A

L

L

T

Q

L

G

F

L

I

N

E

S

V

I

A

L

K

E

E

S

W

K

Q

E

A

G

A

K

G

L

K

K

L

L

P

-

D

D

R

F

S

S

A

A

W

W

L

R

A

Q

E

E

C

L

R

T

E

E

R

Q

K

K

R

-

T

-

L

V

Q

K

R

H

R

P

T

L

N

N

F

F

T

K

E

T

V

F

-

G

-

D

P

A

I

I

K

P

P

P

Q

Q

R

Y

V

A

Y

I

E

Q

E

V

M

L

N

D

K

E

A

L

F

T

D

N

R

G

N

N

T

A

C

I

Y

I

V

S

S

T

T

G

I

V

G

G

L

Q

S

H

Q

Q

I

M

A

W

A

A

A

A

Q

Q

F

Y

L

Y

H

K

V

Y

Y

R

K

K

P

P

R

R

H

Q

W

W

I

L

N

T

C

S

G

G

Q

G

A

L

G

G

P

A

L

M

G

G

W

F

T

G

I

L

P

P

A

A

A

A

L

I

G

G

V

A

C

A

A

V

A

G

D

R

P

P

Q

D

R

E

K

V

V

V

V

V

A

D

I

I

S

D

G

G

D

D

Y

G

D

S

F

F

Q

I

F

M

M

N

I

V

E

Q

E

E

L

L

A

A

V

T

G

I

A

K

Q

V

F

E

N

N

L

L

P

P

Y

V

L

K

H

I

V

M

V

L

V

L

N

N

N

N

S

Q

Y

H

L

L

G

G

L

M

I

V

R

V

Q

Q

A

W

Q

E

R

D

G

R

F

F

C161 (= C72) modified: Disulfide link with 307
P194 (= P105) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ V214) modified: Disulfide link with 161

6lpiB Crystal structure of ahas holo-enzyme (see paper)
34% identity, 81% coverage: 11:486/591 of query aligns to 13:468/539 of 6lpiB

query
sites
6lpiB

V

V

Y

H

V

F

L

L

E

E

K

Q

E

Q

G

G

C

I

L

K

Q

I

A

V

F

T

G

G

V
x
I

P

P

G
|
G

A
x
G

A
x
S

I
|
I

N

L

P

P

F

V

Y

Y

A

D

A

A

L

L

K

S

R

Q

H

S

G

T

G

Q

V

I

K

R

H

H

V

I

L

L

A

A

R

R

H

H

V
x
E

E

Q

G

G

A

A

S

G

H

F

M

I

A

A

E

Q

G

G

Y

M

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active site: I27 (≠ V25), G29 (= G27), G30 (≠ A28), S31 (≠ A29), I32 (= I30), E53 (≠ V51), C76 (≠ T75), F115 (= F114), Q116 (= Q115), E117 (≠ A116), K165 (≠ F164), M256 (≠ L257), A283 (≠ H285), V375 (≠ I393), G401 (= G419), M403 (≠ L421), D428 (= D446), N455 (= N473), A457 (≠ Y475), L458 (= L476), L460 (= L478), V461 (≠ I479), Q464 (≠ A482)
binding flavin-adenine dinucleotide: R155 (= R154), G212 (= G211), G213 (= G212), G214 (= G213), T236 (= T237), L237 (= L238), M238 (= M239), L254 (≠ V255), M256 (≠ L257), H257 (≠ Q258), G276 (= G278), A277 (≠ N279), R278 (= R280), D280 (≠ A282), R282 (= R284), A283 (≠ H285), D300 (= D302), I301 (= I303), D319 (= D321), V320 (≠ A322), M380 (≠ I398), G398 (= G416)
binding magnesium ion: D428 (= D446), N455 (= N473)
binding thiamine diphosphate: E53 (≠ V51), C76 (≠ T75), P79 (= P78), G376 (= G394), Q377 (≠ L395), H378 (≠ S396), G401 (= G419), M403 (≠ L421), G427 (= G445), D428 (= D446), G429 (≠ Y447), S430 (≠ D448), M433 (≠ F451), N455 (= N473), A457 (≠ Y475), L458 (= L476), G459 (= G477), L460 (= L478), V461 (≠ I479)

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
35% identity, 82% coverage: 5:486/591 of query aligns to 92:572/664 of P09114

query
sites
P09114

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