SitesBLAST

C98 (= C101) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
C100 (= C103) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
C125 (= C127) mutation to S: Loss of activity. Strong decrease in zinc content.
H127 (≠ A129) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
H129 (≠ L131) mutation to Q: No change in activity or in zinc content.
H131 (≠ P133) mutation to Q: No change in activity or in zinc content.
H132 (≠ G134) mutation to Q: No change in activity or in zinc content.
C138 (≠ L140) mutation to S: No change in activity or in zinc content.
S239 (= S244) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.

4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
37% identity, 81% coverage: 11:252/297 of query aligns to 6:232/380 of 4g6zA

query
sites
4g6zA

R
|
R

F

F

A
|
A

P

P

S
|
S

P

P

S

T

G

G

P

F

L

I

H

H

A

L

G

G

S

N

L

I

V

R

A

S

A

A

M

L

A

Y

S

P

Y

W

L

A

D

F

A

A

K

R

A

K

H

M

D

K

G

G

R

T

W

F

L

V

L

L

R

R

M

I

E
|
E

D

D

I

T

D

D

E

V

A

E

R

R

S

S

A

S

A

Q

G

E

A

A

A

V

A

D

D

A

I

I

V

L

H

E

T

G

L

M

A

A

A

W

L

L

G

G

M

L

Q

D

W

Y

D

D

G

E

P

G

I

P

R

Y

V

Y

Q

Q

S

M

E

Q

S

R

L

M

P

D

D

R

Y

Y

Q

R

A

E

A

V

C

L

A

A

R

Q

L

M

-

Q

-

E

D

K

G

G

L

L

V

V

Y

Y

P

P

C

C

G

Y

C

M

T

R

R

-

E

-

I

-

A

-

D

-

S

-

G

-

V

-

G

-

I

-

A

-

D

-

G

-

A

-

I

-

Y

Y

P

D

G

G

T

T

C

W

R

R

A

P

G

-

L

-

A

E

P

P

G

G

K

K

I

V

-

L

-

P

-

E

-

P

-

A

-

G

-

V

A

A

R

P

A

V

L

L

R

R

V

F

R

R

V

N

P

P

D

L

A

T

G

G

T

T

A

-

S

-

E

-

F

-

I

V

Q

A

F

W

E

D

D

D

R

A

W

V

L

K

G

G

R

R

Q

-

S

V

Q

E

Y

I

L

S

A

N

R

E

E

E

A

L

G

D

D

D

F

L

V

V

L

V

K

A

R

R

A

P

D

D

G

G

Y

T

W

P

A

M

Y

Y

Q

N

L

F

A

C

V

V

D

D

A

L

L

D

Q

M

G

G

V

I

T

T

H

H

V

V

V

I

R
|
R

G

G

A

D

D

D

L
x
H

L

V

E

N

S

N

T

T

A

P

R

R

Q

Q

I

I

Y

N

L

I

Q

L

R

R

L

A

L

L

G

G

Y

G

P

E

V

V

P

P

V

V

Y

Y

L

A

H

H

V

L

P

P

L
x
T

V

V

C

L

T

N

A

E

D

Q

G

G

E

E

K

K

L

M

S

S

K
|
K

Q

R

H

H

G

G

A

A

Q

M

A

S

L

V

active site: S10 (= S15), T214 (≠ L234), K225 (= K245)
binding glutamic acid: R6 (= R11), A8 (= A13), S10 (= S15), E42 (= E47), R184 (= R204), H188 (≠ L208)

Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
35% identity, 81% coverage: 11:252/297 of query aligns to 6:258/485 of Q8DLI5

query
sites
Q8DLI5

R
|
R

F

L

A

A

P

P

S

S

P

P

S

T

G

G

P

N

L

L

H

H

A

I

G

G

S

T

L

A

V

R

A

T

A

A

M

V

A

F

S

N

Y

W

L

L

D

Y

A

A

K

R

A

H

H

R

D

G

G

G

R

K

W

F

L

I

L

L

R

R

M

I

E

E

D

D

I

T

D

D

E

R

A

E

R

R

S

S

A

R

A

P

G

E

A

Y

A

T

A

E

D

N

I

I

V

L

H

E

T

G

L

L

A

Q

A

W

L

L

G

G

M

L

Q

T

W

W

D

D

G

E

P

G

I

P

R

Y

V

F

Q

Q

S

S

E

D

S

R

L

L

P

D

D

L

Y

Y

Q

R

A

Q

A

A

C

I

A

Q

R

T

L

L

-

L

-

D

D

K

G

G

L

L

V

A

Y

Y

P

Y

C

C

G

Y

C

C

T

T

R

P

R

E

E

E

I

L

A

E

D

A

S

L

G

R

V

A

G

E

I

Q

A

K

A

A

D

K

G

G

A

Q

A

A

-

P

-

R

-

Y

-

D

-

N

-

R

-

H

-

R

-

H

I

L

Y

T

P

P

G

E

T

E

C

Q

R

A

A

A

G

F

L

E

A

A

P

A

G

G

K

R

I

T

A

P

R

-

A

V

L

I

R

R

V

F

R

K

V

I

P

E

D

D

A

-

G

-

T

-

A

-

S

D

E

R

F

Q

I

I

Q

E

F

W

E

Q

D

D

R

L

W

V

L

R

G

G

R

R

Q

V

S

S

Q

W

Y

Q

L

G

A

A

R

D

E

L

A

G

G

G

D

D

F

M

V

V

L

I

K

A

R

R

A

A

D

A

-

P

-

R

-

G

-

E

-

I

G

G

Y

Y

W

P

A

L

Y
|
Y

Q

N

L

L

A

V

V

V

D

D

A

I

L

A

Q

M

G

G

V

I

T

T

H

D

V

V

V

I

R

R

G

G

A

E

D

D

L

H

L

I

E

G

S

N

T

T

A

P

R

K

Q

Q

I

I

Y

L

L

L

Q

Y

R

E

L

A

L

L

G

G

Y

A

P

T

V

P

P

P

V

N

Y

F

L

A

H

H

V

T

P

P

L

L

V

I

C

L

T

N

A

S

D

T

G

G

E

Q

K

K

L

L

S

S

K

K

Q

R

H

D

G

G

A

V

Q

T

A

S

L

I

R6 (= R11) binding L-glutamate
Y192 (= Y186) binding L-glutamate

2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
35% identity, 81% coverage: 11:252/297 of query aligns to 5:257/484 of 2cfoA

query
sites
2cfoA

R
|
R

F

L

A

A

P

P

S
|
S

P

P

S

T

G

G

P

N

L

L

H

H

A

I

G

G

S

T

L

A

V

R

A

T

A

A

M

V

A

F

S

N

Y

W

L

L

D

Y

A

A

K

R

A

H

H

R

D

G

G

G

R

K

W

F

L

I

L

L

R

R

M

I

E

E

D

D

I

T

D

D

E

R

A

E

R

R

S

S

A

R

A

P

G

E

A

Y

A

T

A

E

D

N

I

I

V

L

H

E

T

G

L

L

A

Q

A

W

L

L

G

G

M

L

Q

T

W

W

D

D

G

E

P

G

I

P

R

Y

V

F

Q

Q

S

S

E

D

S

R

L

L

P

D

D

L

Y

Y

Q

R

A

Q

A

A

C

I

A

Q

R

T

L

L

-

L

-

D

D

K

G

G

L

L

V

A

Y

Y

P

Y

C

C

G

Y

C

C

T

T

R

P

R

E

E

E

I

L

A

E

D

A

S

L

G

R

V

A

G

E

I

Q

A

K

A

A

D

K

G

G

A

Q

A

A

I

P

Y

R

P

Y

G

D

T

N

C

R

R

H

A

R

G

H

L

L

A

T

P

P

G

E

K

E

I

Q

A

A

R

A

-

F

-

E

-

A

-

G

-

R

-

T

-

P

A

V

L

I

R

R

V

F

R

K

V

I

P

E

D

D

A

-

G

-

T

-

A

-

S

D

E

R

F

Q

I

I

Q

E

F

W

E

Q

D

D

R

L

W

V

L

R

G

G

R

R

Q

V

S

S

Q

W

Y

Q

L

G

A

A

R

D

E

L

A

G

G

G

D

D

F

M

V

V

L

I

K

A

R

R

A

A

D

A

-

P

-

R

-

G

-

E

-

I

G

G

Y

Y

W

P

A

L

Y
|
Y

Q

N

L

L

A

V

V

V

D

D

A

I

L

A

Q

M

G

G

V

I

T

T

H

D

V

V

V

I

R
|
R

G

G

A

E

D

D

L
x
H

L

I

E

G

S

N

T

T

A

P

R

K

Q

Q

I

I

Y

L

L

L

Q

Y

R

E

L

A

L

L

G

G

Y

A

P

T

V

P

P

P

V

N

Y

F

L

A

H

H

V

T

P

P

L
|
L

V

I

C

L

T

N

A

S

D

T

G

G

E

Q

K

K

L

L

S

S

K
|
K

Q

R

H

D

G

G

A

V

Q

T

A

S

L

I

active site: S9 (= S15), L239 (= L234), K250 (= K245)
binding glutamic acid: R5 (= R11), Y191 (= Y186), R209 (= R204), H213 (≠ L208)

3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
30% identity, 82% coverage: 11:253/297 of query aligns to 106:343/564 of 3al0C

query
sites
3al0C

R
|
R

F

F

A
|
A

P
|
P

S
|
S

P

P

S

T

G

G

P

H

L

L

H

H

A

V

G
|
G

S

G

L

A

V

R

A
x
T

A

A

M

L

A

F

S

N

Y

W

L

M

D

F

A

A

K

R

A

K

H

E

D

G

G

G

R

K

W

F

L

I

L

L

R

R

M

I

E
|
E

D

D

I
x
T

D
|
D

E

T

A

E

R
|
R

S

S

A

S

A

R

G

E

A

Y

A

E

A

Q

D

Q

I

I

V

L

H

E

T

S

L

L

A

R

A

W

L

C

G

G

M

L

Q

D

W

W

D

D

-

E

-

G

-

P

-

D

-

I

-

G

-

D

-

F

G

G

P

P

I

Y

R

R

V

-

Q

Q

S

S

E

E

S

R

L

L

P

E

D

I

Y

Y

Q

R

A

E

A

Y

C

A

A

E

R

K

L

L

D

V

G

E

-

D

-

K

-

R

-

A

-

Y

L

V

V

V

Y
|
Y

P

D

C

K

G

E

C

D

T
x
P

R

S

R

K

E

E

I

L

A

F

D

T

S

T

G

Y

V

E

G

Y

I

P

A

H

A

E

D

Y

G

K

A

E

A

K

I

G

Y

H

P

P

G

V

T

T

C

I

R

K

A

F

G

K

L

V

A

L

P

P

G

G

K

K

I

T

A

S

R

-

A

-

L

-

R

-

V

-

R

-

V

-

P

-

D

-

A

-

G

-

T

-

A

-

S

-

E

-

F

-

I

-

Q

-

F

F

E

E

D

D

R

L

W

L

L
x
K

G

G

R

Y

Q
x
M

S

-

Q

E

Y

F

L

D

A

N

R

S

E

T

A

L

G

E

D

D

F

F

V
x
I

L

I

K

M

R
x
K

A
x
S

D

N

G

G

Y

F

W

P

A

T

Y
|
Y

Q

N

L

F

A

A

V

V

D

D

A

H

L

L

Q

M

G

R

V

I

T

S

H

H

V

V

V

F

R
|
R

G
|
G

A

E

D
|
D

L
x
H

L
|
L

E
x
S

S
x
N

T

T

A

P

R
x
K

Q

Q

I

L

Y

M

L

I

Q

Y

R

E

L

A

L

F

G

G

Y

W

P

E

V

A

P

P

V

V

Y

F

L

M

H

H

V

I

P

P

L
|
L

V
x
I

C

L

T

G

A

S

D

D

G
x
R

E

T

K
x
P

L
|
L

S

S

K
|
K

Q

R

H

H

G

G

A

A

Q

T

A

S

L

V

D

E

active site: S110 (= S15), K335 (= K245)
binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R11), A108 (= A13), P109 (= P14), G118 (= G23), T122 (≠ A27), E142 (= E47), Y276 (= Y186), R294 (= R204), G295 (= G205), D297 (= D207), H298 (≠ L208), L324 (= L234), I325 (≠ V235), L333 (= L243)
binding : T144 (≠ I49), D145 (= D50), R148 (= R53), Y208 (= Y99), P213 (≠ T104), K252 (≠ L161), M255 (≠ Q164), I266 (≠ V176), K269 (≠ R179), S270 (≠ A180), Y276 (= Y186), D297 (= D207), H298 (≠ L208), L299 (= L209), S300 (≠ E210), N301 (≠ S211), K304 (≠ R214), R330 (≠ G240), P332 (≠ K242)

Sites not aligning to the query:

binding : 363, 364, 365, 370, 387, 389, 391, 392, 397, 400, 407, 446, 447, 453, 457, 509, 520, 524, 527, 535, 536, 538, 539

4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
33% identity, 81% coverage: 11:252/297 of query aligns to 5:260/485 of 4griB

query
sites
4griB

R
|
R

F

Y

A
|
A

P

P

S
|
S

P

P

S

T

G

G

P

L

L

Q

H

H

A

I

G

G

S

G

L

I

V

R

A

T

A

A

M

L

A

F

S

N

Y

Y

L

F

D

F

A

A

K

K

A

S

H

C

D

G

G

G

R

K

W

F

L

L

R

R

M

I

E
|
E

D

D

I

T

D

D

E

Q

A

S

R

R

S

Y

A

S

A

P

G

E

A

A

A

E

A

N

D

D

I

L

V

Y

H

S

T

S

L

L

A

K

A

W

L

L

G

G

M

I

Q

S

W

F

D

D

-

E

G

G

P

P

I

V

-

V

-

G

-

D

-

Y

-

A

-

P

R

Y

V

V

Q

Q

S

S

E

Q

S

R

L

S

P

A

D

I

Y

Y

Q

K

A

Q

A

Y

C

A

A

K

R

Y

L

L

-

I

-

E

D

S

G

G

L

H

V

A

Y

Y

P

Y

C
|
C

G

Y

C
|
C

T

S

R

P

R

E

E

R

I

L

A

-

D

-

S

E

G

R

V

I

G

K

I

K

A

I

A

Q

D

N

G

I

A

N

A

K

I

M

Y

P

P

P

G

G

T
x
Y

-

D

-

R

-

H

C
|
C

R

R

A

-

G

N

L

L

A

S

P

N

G

E

K

E

I

V

A

E

R

N

A

A

L

L

-

I

-

K

-

I

-

K

-

P

-

V

R

R

V

F

R

K

V

I

P

P

D

L

A

E

G

G

T

D

A

T

S

S

E

-

F

-

I

-

Q

-

F

F

E

D

D

D

R

I

W

L

L

L

G

G

R

R

Q

I

S

T

Q

W

Y

A

L

N

A

K

R

D

E

I

A

S

G

P

D

D

F

P

V

V

L

I

K

L

R

K

A

S

D

D

G

G

Y

L

W

P

A

T

Y
|
Y

Q

H

L

L

A

A

V

N

V

V

D

D

A

Y

L

L

Q

M

G

K

V

I

T

T

H

H

V

V

V

L

R
|
R

G

A

A

Q

D

E

L
x
W

L

V

E

S

S

S

T

G

A

P

R

L

Q

H

I

V

Y

L

L

L

Q

Y

R

K

L

A

L

F

G

K

Y

W

P

K

V

P

P

P

V

I

Y

Y

L

C

H

H

V

L

P

P

L

M

V

V

C

M

T

G

A

N

D

D

G

G

E

Q

K

K

L

L

S

S

K
|
K

Q

R

H

H

G

G

A

S

Q

T

A

A

L

L

active site: S9 (= S15), K253 (= K245)
binding glutamic acid: R5 (= R11), A7 (= A13), S9 (= S15), E41 (= E47), Y194 (= Y186), R212 (= R204), W216 (≠ L208)
binding zinc ion: C105 (= C101), C107 (= C103), Y128 (≠ T126), C132 (= C127)

2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
35% identity, 88% coverage: 9:268/297 of query aligns to 3:262/468 of 2cv2A

query
sites
2cv2A

V

V

G

T

R
|
R

F

I

A
|
A

P

P

S
|
S

P

P

S

T

G

G

P

D

L

P

H

H

A

V

G
|
G

S

T

L

A

V

Y

A
x
I

A

A

M

L

A

F

S

N

Y

Y

L

A

D

W

A

A

K

R

A

R

H

N

D

G

G

G

R

R

W

F

L

I

L

V

R

R

M

I

E
|
E

D

D

I
x
T

D
|
D

E

R

A

A

R
|
R

S

Y

A

V

A

P

G

G

A

A

A

E

D

R

I

I

V

L

H

A

T

A

L

L

A

K

A

W

L

L

G

G

M

L

Q

S

W

Y

D

D

-

E

-

G

-

P

-

D

-

V

-

G

-

P

-

H

G

G

P

P

I

Y

R

R

V

-

Q

Q

S

S

E

E

S

R

L

L

P

P

D

L

Y

Y

Q

Q

A

K

A

Y

C

A

A

E

R

E

L

L

-

L

-

K

D

R

G

G

L

W

V

A

Y

Y

P

R

C

A

G

F

C

E

T

T

R

P

R

E

E

E

-

L

-

E

-

Q

I

I

A

R

D

K

S

E

G

K

V

G

G

G

I

Y

A

D

A

G

D

R

G

A

A

R

A

N

I

I

Y

P

P

P

G

E

T

E

C

A

R

E

A

E

G

R

L

A

A

R

P

R

G

G

K

E

I

-

A

P

R

H

A
x
V

L

I

R

R

V

L

R

K

V

V

P

P

D

R

A

P

G

G

T

T

A

T

S

-

E

-

F

-

I

-

Q

E

F

V

E

K

D

D

R

E

W

L

L
x
R

G

G

R

-

Q

V

S

V

Q

V

Y
|
Y

L

D

A

N

R

Q

E
|
E

A

I

G

P

D

D

F

V

V
|
V

L

L

K

L

R
x
K

A
x
S

D

D

G

G

Y

Y

W

P

A

T

Y
|
Y

Q

H

L

L

A

A

V

N

V

V

D

D

A

H

L

L

Q

M

G

G

V

V

T

T

H

D

V

V

V

I

R
|
R

G
x
A

A
x
E

D
x
E

L
x
W

L

L

E
x
V

S

S

T

T

A

P

R

I

Q

H

I

V

Y

L

L

L

Q

Y

R

R

L

A

L

F

G

G

Y

W

P

E

V

A

P

P

V

R

Y

F

L

Y

H

H

V

M

P

P

L
|
L

V
x
L

C
x
R

T

N

A

P

D

D

G
x
K

E

T

K

K

L

I

S

S

K
|
K

Q

R

H

K

G

S

A

H

Q

T

A

S

L

L

D

D

L

-

Q

-

P

-

L

-

P

-

E

-

L

W

Q

Y

R

K

A

A

A

E

G

G

F

F

L

L

active site: K246 (= K245)
binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R11), A7 (= A13), S9 (= S15), G17 (= G23), I21 (≠ A27), E41 (= E47), Y187 (= Y186), R205 (= R204), A206 (≠ G205), E208 (≠ D207), W209 (≠ L208), L235 (= L234), L236 (≠ V235)
binding : S9 (= S15), T43 (≠ I49), D44 (= D50), R47 (= R53), V145 (≠ A139), R163 (≠ L161), Y168 (= Y167), E172 (= E171), V177 (= V176), K180 (≠ R179), S181 (≠ A180), Y187 (= Y186), E207 (≠ A206), E208 (≠ D207), W209 (≠ L208), V211 (≠ E210), R237 (≠ C236), K241 (≠ G240)

Sites not aligning to the query:

binding : 272, 273, 274, 282, 299, 303, 304, 309, 312, 319, 357, 358, 417, 432, 435, 442, 443, 444, 446, 447, 448

2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
35% identity, 88% coverage: 9:268/297 of query aligns to 3:262/468 of 2cv1A

query
sites
2cv1A

V

V

G

T

R
|
R

F

I

A
|
A

P
|
P

S
|
S

P

P

S

T

G

G

P

D

L

P

H

H

A

V

G
|
G

S
x
T

L

A

V

Y

A
x
I

A

A

M

L

A

F

S

N

Y

Y

L

A

D

W

A

A

K

R

A

R

H

N

D

G

G

G

R

R

W

F

L

I

L

V

R

R

M

I

E
|
E

D

D

I
x
T

D
|
D

E

R

A

A

R
|
R

S

Y

A

V

A

P

G

G

A

A

A

E

D

R

I

I

V

L

H

A

T

A

L

L

A

K

A

W

L

L

G

G

M

L

Q

S

W

Y

D

D

-

E

-

G

-

P

-

D

-

V

-

G

-

P

-

H

G

G

P

P

I

Y

R

R

V

-

Q

Q

S

S

E

E

S

R

L

L

P

P

D

L

Y

Y

Q

Q

A

K

A

Y

C

A

A

E

R

E

L

L

-

L

-

K

D

R

G

G

L

W

V

A

Y

Y

P

R

C

A

G

F

C

E

T

T

R

P

R

E

E

E

-

L

-

E

-

Q

I

I

A

R

D

K

S

E

G

K

V

G

G

G

I

Y

A

D

A

G

D

R

G

A

A

R

A

N

I

I

Y

P

P

P

G

E

T

E

C

A

R

E

A

E

G

R

L

A

A

R

P

R

G

G

K

E

I

-

A

P

R

H

A
x
V

L

I

R

R

V

L

R

K

V

V

P

P

D

R

A

P

G

G

T

T

A

T

S

-

E

-

F

-

I

-

Q

E

F

V

E

K

D

D

R

E

W

L

L
x
R

G

G

R

-

Q

V

S
x
V

Q

V

Y

Y

L

D

A

N

R

Q

E
|
E

A

I

G

P

D

D

F

V

V
|
V

L

L

K

L

R
x
K

A
x
S

D

D

G

G

Y

Y

W

P

A

T

Y
|
Y

Q

H

L

L

A

A

V

N

V

V

D

D

A

H

L

L

Q

M

G

G

V

V

T

T

H

D

V

V

V

I

R
|
R

G
x
A

A
x
E

D
x
E

L
x
W

L

L

E
x
V

S

S

T

T

A

P

R

I

Q

H

I

V

Y

L

L

L

Q

Y

R

R

L

A

L

F

G

G

Y

W

P

E

V

A

P

P

V

R

Y

F

L

Y

H

H

V

M

P

P

L
|
L

V
x
L

C
x
R

T

N

A

P

D

D

G
x
K

E

T

K
|
K

L

I

S

S

K
|
K

Q

R

H

K

G

S

A

H

Q

T

A

S

L

L

D

D

L

-

Q

-

P

-

L

-

P

-

E

-

L

W

Q

Y

R

K

A

A

A

E

G

G

F

F

L

L

active site: K246 (= K245)
binding adenosine-5'-triphosphate: P8 (= P14), S9 (= S15), G17 (= G23), T18 (≠ S24), I21 (≠ A27), R47 (= R53), A206 (≠ G205), W209 (≠ L208), L235 (= L234), L236 (≠ V235)
binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R11), A7 (= A13), E41 (= E47), Y187 (= Y186), R205 (= R204), W209 (≠ L208)
binding : S9 (= S15), E41 (= E47), T43 (≠ I49), D44 (= D50), R47 (= R53), V145 (≠ A139), R163 (≠ L161), V166 (≠ S165), E172 (= E171), V177 (= V176), K180 (≠ R179), S181 (≠ A180), Y187 (= Y186), E207 (≠ A206), E208 (≠ D207), W209 (≠ L208), V211 (≠ E210), R237 (≠ C236), K241 (≠ G240), K243 (= K242)

Sites not aligning to the query:

binding : 273, 274, 276, 282, 299, 303, 304, 309, 312, 319, 357, 358, 417, 427, 432, 435, 442, 443, 444, 446, 447, 448

2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
35% identity, 88% coverage: 9:268/297 of query aligns to 3:262/468 of 2cuzA

query
sites
2cuzA

V

V

G

T

R
|
R

F

I

A
|
A

P

P

S
|
S

P

P

S

T

G

G

P

D

L

P

H

H

A

V

G

G

S

T

L

A

V

Y

A

I

A

A

M

L

A

F

S

N

Y

Y

L

A

D

W

A

A

K

R

A

R

H

N

D

G

G

G

R

R

W

F

L

I

L

V

R

R

M

I

E
|
E

D

D

I

T

D

D

E

R

A

A

R

R

S

Y

A

V

A

P

G

G

A

A

A

E

D

R

I

I

V

L

H

A

T

A

L

L

A

K

A

W

L

L

G

G

M

L

Q

S

W

Y

D

D

-

E

-

G

-

P

-

D

-

V

-

G

-

P

-

H

G

G

P

P

I

Y

R

R

V

-

Q

Q

S

S

E

E

S

R

L

L

P

P

D

L

Y

Y

Q

Q

A

K

A

Y

C

A

A

E

R

E

L

L

-

L

-

K

D

R

G

G

L

W

V

A

Y

Y

P

R

C

A

G

F

C

E

T

T

R

P

R

E

E

E

-

L

-

E

-

Q

I

I

A

R

D

K

S

E

G

K

V

G

G

G

I

Y

A

D

A

G

D

R

G

A

A

R

A

N

I

I

Y

P

P

P

G

E

T

E

C

A

R

E

A

E

G

R

L

A

A

R

P

R

G

G

K

E

I

-

A

P

R

H

A

V

L

I

R

R

V

L

R

K

V

V

P

P

D

R

A

P

G

G

T

T

A

T

S

-

E

-

F

-

I

-

Q

E

F

V

E

K

D

D

R

E

W

L

L

R

G

G

R

-

Q

V

S

V

Q

V

Y

Y

L

D

A

N

R

Q

E

E

A

I

G

P

D

D

F

V

V

V

L

L

K

L

R

K

A

S

D

D

G

G

Y

Y

W

P

A

T

Y
|
Y

Q

H

L

L

A

A

V

N

V

V

D

D

A

H

L

L

Q

M

G

G

V

V

T

T

H

D

V

V

V

I

R
|
R

G

A

A

E

D

E

L

W

L

L

E

V

S

S

T

T

A

P

R

I

Q

H

I

V

Y

L

L

L

Q

Y

R

R

L

A

L

F

G

G

Y

W

P

E

V

A

P

P

V

R

Y

F

L

Y

H

H

V

M

P

P

L

L

V

L

C

R

T

N

A

P

D

D

G

K

E

T

K

K

L

I

S

S

K
|
K

Q

R

H

K

G

S

A

H

Q

T

A

S

L

L

D

D

L

-

Q

-

P

-

L

-

P

-

E

-

L

W

Q

Y

R

K

A

A

A

E

G

G

F

F

L

L

active site: K246 (= K245)
binding glutamic acid: R5 (= R11), A7 (= A13), S9 (= S15), E41 (= E47), Y187 (= Y186), R205 (= R204)

1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
35% identity, 88% coverage: 9:268/297 of query aligns to 3:262/468 of 1n78A

query
sites
1n78A

V

V

G

T

R
|
R

F

I

A
|
A

P
|
P

S
|
S

P

P

S

T

G

G

P

D

L

P

H

H

A

V

G
|
G

S
x
T

L

A

V

Y

A
x
I

A

A

M

L

A

F

S

N

Y

Y

L

A

D

W

A

A

K

R

A

R

H

N

D

G

G

G

R

R

W

F

L

I

L

V

R

R

M

I

E
|
E

D

D

I
x
T

D
|
D

E

R

A

A

R
|
R

S

Y

A

V

A

P

G

G

A

A

A

E

D

R

I

I

V

L

H

A

T

A

L

L

A

K

A

W

L

L

G

G

M

L

Q

S

W

Y

D

D

-

E

-

G

-

P

-

D

-

V

-

G

-

P

-

H

G

G

P

P

I

Y

R

R

V

-

Q

Q

S

S

E

E

S

R

L

L

P

P

D

L

Y

Y

Q

Q

A

K

A

Y

C

A

A

E

R

E

L

L

-

L

-

K

D

R

G

G

L

W

V

A

Y

Y

P

R

C

A

G

F

C

E

T

T

R

P

R

E

E

E

-

L

-

E

-

Q

I

I

A

R

D

K

S

E

G

K

V

G

G

G

I

Y

A

D

A

G

D

R

G

A

A

R

A

N

I

I

Y

P

P

P

G

E

T

E

C

A

R

E

A

E

G

R

L

A

A

R

P

R

G

G

K

E

I

-

A

P

R

H

A
x
V

L

I

R

R

V

L

R

K

V

V

P

P

D

R

A

P

G

G

T

T

A

T

S

-

E

-

F

-

I

-

Q

E

F

V

E

K

D

D

R

E

W

L

L
x
R

G

G

R

-

Q

V

S
x
V

Q

V

Y
|
Y

L

D

A

N

R

Q

E
|
E

A

I

G

P

D

D

F

V

V
|
V

L

L

K

L

R
x
K

A
x
S

D

D

G

G

Y

Y

W

P

A

T

Y
|
Y

Q

H

L

L

A

A

V
x
N

V

V

D

D

A

H

L

L

Q

M

G

G

V

V

T

T

H

D

V

V

V

I

R
|
R

G
x
A

A
x
E

D
x
E

L
x
W

L
|
L

E
x
V

S

S

T

T

A

P

R

I

Q

H

I

V

Y

L

L

L

Q

Y

R

R

L

A

L

F

G

G

Y

W

P

E

V

A

P

P

V

R

Y

F

L

Y

H

H

V

M

P

P

L
|
L

V
x
L

C
x
R

T

N

A

P

D

D

G
x
K

E

T

K

K

L

I

S

S

K
|
K

Q

R

H

K

G

S

A

H

Q

T

A

S

L

L

D

D

L

-

Q

-

P

-

L

-

P

-

E

-

L

W

Q

Y

R

K

A

A

A

E

G

G

F

F

L

L

active site: K246 (= K245)
binding glutamol-amp: R5 (= R11), A7 (= A13), P8 (= P14), S9 (= S15), G17 (= G23), T18 (≠ S24), I21 (≠ A27), E41 (= E47), Y187 (= Y186), N191 (≠ V190), R205 (= R204), A206 (≠ G205), E208 (≠ D207), W209 (≠ L208), L235 (= L234), L236 (≠ V235)
binding : S9 (= S15), T43 (≠ I49), D44 (= D50), R47 (= R53), V145 (≠ A139), R163 (≠ L161), V166 (≠ S165), Y168 (= Y167), E172 (= E171), V177 (= V176), K180 (≠ R179), S181 (≠ A180), Y187 (= Y186), E207 (≠ A206), E208 (≠ D207), W209 (≠ L208), L210 (= L209), V211 (≠ E210), R237 (≠ C236), K241 (≠ G240)

Sites not aligning to the query:

binding : 273, 274, 282, 297, 303, 304, 309, 312, 319, 357, 358, 417, 427, 432, 435, 442, 443, 444, 446, 447, 448

1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
35% identity, 88% coverage: 9:268/297 of query aligns to 3:262/468 of 1j09A

query
sites
1j09A

V

V

G

T

R
|
R

F

I

A
|
A

P

P

S
|
S

P

P

S

T

G

G

P

D

L

P

H
|
H

A

V

G

G

S

T

L

A

V

Y

A

I

A

A

M

L

A

F

S

N

Y

Y

L

A

D

W

A

A

K

R

A

R

H

N

D

G

G

G

R

R

W

F

L

I

L

V

R

R

M

I

E
|
E

D

D

I

T

D

D

E

R

A

A

R

R

S

Y

A

V

A

P

G

G

A

A

A

E

D

R

I

I

V

L

H

A

T

A

L

L

A

K

A

W

L

L

G

G

M

L

Q

S

W

Y

D

D

-

E

-

G

-

P

-

D

-

V

-

G

-

P

-

H

G

G

P

P

I

Y

R

R

V

-

Q

Q

S

S

E

E

S

R

L

L

P

P

D

L

Y

Y

Q

Q

A

K

A

Y

C

A

A

E

R

E

L

L

-

L

-

K

D

R

G

G

L

W

V

A

Y

Y

P

R

C

A

G

F

C

E

T

T

R

P

R

E

E

E

-

L

-

E

-

Q

I

I

A

R

D

K

S

E

G

K

V

G

G

G

I

Y

A

D

A

G

D

R

G

A

A

R

A

N

I

I

Y

P

P

P

G

E

T

E

C

A

R

E

A

E

G

R

L

A

A

R

P

R

G

G

K

E

I

-

A

P

R

H

A

V

L

I

R

R

V

L

R

K

V

V

P

P

D

R

A

P

G

G

T

T

A

T

S

-

E

-

F

-

I

-

Q

E

F

V

E

K

D

D

R

E

W

L

L

R

G

G

R

-

Q

V

S

V

Q

V

Y

Y

L

D

A

N

R

Q

E

E

A

I

G

P

D

D

F

V

V

V

L

L

K

L

R

K

A

S

D

D

G

G

Y

Y

W

P

A

T

Y
|
Y

Q

H

L

L

A

A

V
x
N

V

V

D

D

A

H

L

L

Q

M

G

G

V

V

T

T

H

D

V

V

V

I

R
|
R

G

A

A

E

D
x
E

L
x
W

L

L

E

V

S

S

T

T

A

P

R

I

Q

H

I

V

Y

L

L

L

Q

Y

R

R

L

A

L

F

G

G

Y

W

P

E

V

A

P

P

V

R

Y

F

L

Y

H

H

V

M

P

P

L
|
L

V
x
L

C

R

T

N

A

P

D

D

G

K

E

T

K
|
K

L
x
I

S
|
S

K
|
K

Q
x
R

H

K

G

S

A

H

Q

T

A

S

L

L

D

D

L

-

Q

-

P

-

L

-

P

-

E

-

L

W

Q

Y

R

K

A

A

A

E

G

G

F

F

L

L

active site: K246 (= K245)
binding adenosine-5'-triphosphate: H15 (= H21), E208 (≠ D207), L235 (= L234), L236 (≠ V235), K243 (= K242), I244 (≠ L243), S245 (= S244), K246 (= K245), R247 (≠ Q246)
binding glutamic acid: R5 (= R11), A7 (= A13), S9 (= S15), E41 (= E47), Y187 (= Y186), N191 (≠ V190), R205 (= R204), W209 (≠ L208)

P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
35% identity, 88% coverage: 9:268/297 of query aligns to 3:262/468 of P27000

query
sites
P27000

V

V

G

T

R

R

F

I

A

A

P

P

S

S

P

P

S

T

G

G

P

D

L

P

H

H

A

V

G

G

S

T

L

A

V

Y

A

I

A

A

M

L

A

F

S

N

Y

Y

L

A

D

W

A

A

K

R

A

R

H

N

D

G

G

G

R

R

W

F

L

I

L

V

R

R

M

I

E

E

D

D

I

T

D

D

E

R

A

A

R

R

S

Y

A

V

A

P

G

G

A

A

A

E

D

R

I

I

V

L

H

A

T

A

L

L

A

K

A

W

L

L

G

G

M

L

Q

S

W

Y

D

D

-

E

-

G

-

P

-

D

-

V

-

G

-

P

-

H

G

G

P

P

I

Y

R

R

V

-

Q

Q

S

S

E

E

S

R

L

L

P

P

D

L

Y

Y

Q

Q

A

K

A

Y

C

A

A

E

R

E

L

L

-

L

-

K

D

R

G

G

L

W

V

A

Y

Y

P

R

C

A

G

F

C

E

T

T

R

P

R

E

E

E

-

L

-

E

-

Q

I

I

A

R

D

K

S

E

G

K

V

G

G

G

I

Y

A

D

A

G

D

R

G

A

A

R

A

N

I

I

Y

P

P

P

G

E

T

E

C

A

R

E

A

E

G

R

L

A

A

R

P

R

G

G

K

E

I

-

A

P

R

H

A

V

L

I

R

R

V

L

R

K

V

V

P

P

D

R

A

P

G

G

T

T

A

T

S

-

E

-

F

-

I

-

Q

E

F

V

E

K

D

D

R

E

W

L

L

R

G

G

R

-

Q

V

S

V

Q

V

Y

Y

L

D

A

N

R

Q

E

E

A

I

G

P

D

D

F

V

V

V

L

L

K

L

R

K

A

S

D

D

G

G

Y

Y

W

P

A

T

Y

Y

Q

H

L

L

A

A

V

N

V

V

D

D

A

H

L

L

Q

M

G

G

V

V

T

T

H

D

V

V

V

I

R

R

G

A

A

E

D

E

L

W

L

L

E

V

S

S

T

T

A

P

R

I

Q

H

I

V

Y

L

L

L

Q

Y

R

R

L

A

L

F

G

G

Y

W

P

E

V

A

P

P

V

R

Y

F

L

Y

H

H

V

M

P

P

L

L

V

L

C

R

T

N

A

P

D

D

G

K

E

T

K

K

L

I

S

S

K

K

Q

R

H

K

G

S

A

H

Q

T

A

S

L

L

D

D

L

-

Q

-

P

-

L

-

P

-

E

-

L

W

Q

Y

R

K

A

A

A

E

G

G

F

F

L

L

Sites not aligning to the query:

358 R→Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).

1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
35% identity, 88% coverage: 9:268/297 of query aligns to 3:262/468 of 1g59A

query
sites
1g59A

V

V

G

T

R

R

F

I

A

A

P

P

S

S

P

P

S

T

G

G

P

D

L

P

H

H

A

V

G

G

S

T

L

A

V

Y

A

I

A

A

M

L

A

F

S

N

Y

Y

L

A

D

W

A

A

K

R

A

R

H

N

D

G

G

G

R

R

W

F

L

I

L

V

R

R

M

I

E

E

D

D

I

T

D
|
D

E
x
R

A
|
A

R
|
R

S

Y

A

V

A

P

G

G

A

A

A

E

D

R

I

I

V

L

H

A

T

A

L

L

A

K

A

W

L

L

G

G

M

L

Q

S

W

Y

D

D

-

E

-

G

-

P

-

D

-

V

-

A

-

P

-

T

G

G

P

P

I

Y

R

R

V

-

Q

Q

S

S

E

E

S

R

L

L

P

P

D

L

Y

Y

Q

Q

A

K

A

Y

C

A

A

E

R

E

L

L

-

L

-

K

D

R

G

G

L

W

V

A

Y

Y

P

R

C

A

G

F

C

E

T

T

R
x
P

R

E

E

E

-

L

-

E

-

Q

I

I

A

R

D

K

S

E

G

K

V

G

G

G

I

Y

A

D

A

G

D

R

G

A

A

R

A

N

I

I

Y

P

P

P

G

E

T

E

C

A

R

E

A

E

G

R

L

A

A

R

P

R

G

G

K

E

I

-

A

P

R

H

A
x
V

L

I

R

R

V

L

R

K

V

V

P

P

D

R

A

P

G

G

T

T

A

T

S

-

E

-

F

-

I

-

Q

E

F

V

E

K

D

D

R

E

W

L

L
x
R

G

G

R

-

Q

V

S
x
V

Q

V

Y

Y

L

D

A

N

R

Q

E
|
E

A

I

G

P

D

D

F

V

V
|
V

L

L

K

L

R
x
K

A
x
S

D
|
D

G

G

Y

Y

W

P

A

T

Y

Y

Q

H

L

L

A

A

V

N

V

V

D

D

A

H

L

L

Q

M

G

G

V

V

T

T

H

D

V

V

V

I

R

R

G

A

A
x
E

D
x
E

L

W

L

L

E

V

S

S

T

T

A

P

R

I

Q

H

I

V

Y

L

L

L

Q

Y

R

R

L

A

L

F

G

G

Y

W

P

E

V

A

P

P

V

R

Y

F

L

Y

H

H

V

M

P

P

L

L

V

L

C
x
R

T

N

A

P

D

D

G
x
K

E
x
T

K
|
K

L

I

S

S

K

K

Q

R

H

K

G

S

A

H

Q

T

A

S

L

L

D

D

L

-

Q

-

P

-

L

-

P

-

E

-

L

W

Q

Y

R

K

A

A

A

E

G

G

F

F

L

L

binding : D44 (= D50), R45 (≠ E51), A46 (= A52), R47 (= R53), P109 (≠ R105), V145 (≠ A139), R163 (≠ L161), V166 (≠ S165), E172 (= E171), V177 (= V176), K180 (≠ R179), S181 (≠ A180), D182 (= D181), E207 (≠ A206), E208 (≠ D207), R237 (≠ C236), K241 (≠ G240), T242 (≠ E241), K243 (= K242)

Sites not aligning to the query:

binding : 273, 274, 282, 299, 300, 303, 304, 309, 312, 319, 357, 358, 417, 426, 427, 432, 435, 442, 443, 444, 445, 446, 447, 448

8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
31% identity, 87% coverage: 11:269/297 of query aligns to 7:279/488 of 8vc5A

query
sites
8vc5A

R

R

F

I

A

A

P

P

S

S

P

P

S

T

G

G

P

D

L

P

H

H

A

V

G

G

S

T

L

A

V

Y

A

I

A

A

M

L

A

F

S

N

Y

L

L

C

D

F

A

A

K

R

A

Q

H

H

D

G

G

G

R

Q

W

F

L

I

L

L

R

R

M

I

E

E

D

D

I

T

D

D

E

Q

A

L

R

R

S

S

A

T

A

R

G

E

A

S

A

E

A

Q

D

Q

I

I

V

Y

H

D

T

A

L

L

A

R

A

W

L

L

G

G

M

I

Q

E