SitesBLAST
Comparing WP_050465826.1 NCBI__GCF_001189915.1:WP_050465826.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 18 hits to proteins with known functional sites (download)
P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 100% coverage: 3:430/430 of query aligns to 81:509/534 of P53322
- K283 (≠ Q207) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
23% identity, 98% coverage: 1:423/430 of query aligns to 5:428/430 of P0AA76
- Y29 (= Y26) binding D-galactonate
- D31 (= D28) mutation to N: Loss of galactonate transport activity.
- R32 (= R29) binding D-galactonate
- Y64 (= Y61) binding D-galactonate
- E118 (= E115) mutation to Q: Loss of galactonate transport activity.
- W358 (= W350) binding D-galactonate
6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
25% identity, 97% coverage: 8:423/430 of query aligns to 1:409/409 of 6e9nA
6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
25% identity, 97% coverage: 8:423/430 of query aligns to 4:393/393 of 6e9oA
8u3gA Structure of naag-bound sialin (see paper)
23% identity, 86% coverage: 55:423/430 of query aligns to 52:423/427 of 8u3gA
Sites not aligning to the query:
8u3hA Structure of fmoc-leu-oh bound sialin (see paper)
23% identity, 86% coverage: 55:423/430 of query aligns to 50:421/425 of 8u3hA
Sites not aligning to the query:
Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
22% identity, 86% coverage: 55:423/430 of query aligns to 113:484/495 of Q9NRA2
- K136 (≠ R78) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
- H183 (≠ L123) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
- LL 198:199 (≠ IY 138:139) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- IL 266:267 (≠ LT 212:213) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- SSLRN 268:272 (≠ SGIER 214:218) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
- G328 (= G277) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
- P334 (= P283) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
- G371 (vs. gap) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794
Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
20% identity, 69% coverage: 2:298/430 of query aligns to 90:393/570 of Q9C0U9
Sites not aligning to the query:
- 14 modified: Phosphoserine
8uo8A Synaptic vesicle glycoprotein 2B (see paper)
18% identity, 47% coverage: 74:276/430 of query aligns to 85:316/574 of 8uo8A
- binding [(2R)-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-2-propanoyloxy-propyl] propanoate: M167 (≠ L160), I171 (vs. gap), Y185 (≠ L167), S189 (≠ G171), V192 (≠ W174)
- binding (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en: E148 (≠ R137), T159 (≠ V152), S170 (vs. gap)
- binding (4R)-4-(2-chloro-2,2-difluoroethyl)-1-{[(4R)-2-(methoxymethyl)-6-(trifluoromethyl)imidazo[2,1-b][1,3,4]thiadiazol-5-yl]methyl}pyrrolidin-2-one: F134 (≠ L123), W157 (≠ P150), W288 (≠ Y248), Y295 (≠ I255), Y296 (= Y256)
Sites not aligning to the query:
- binding (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en: 449
- binding 1,2-didecanoyl-sn-glycero-3-[phospho-l-serine]: 18, 19, 23, 27
- binding (4R)-4-(2-chloro-2,2-difluoroethyl)-1-{[(4R)-2-(methoxymethyl)-6-(trifluoromethyl)imidazo[2,1-b][1,3,4]thiadiazol-5-yl]methyl}pyrrolidin-2-one: 440, 494, 495, 498, 502, 522, 525, 526
- binding cholesterol hemisuccinate: 442, 443, 462, 492, 493
Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 82% coverage: 55:406/430 of query aligns to 129:486/582 of Q9JI12
- R184 (= R108) mutation R->A,E,K: Greatly lowers L-glutamate transport.
- E191 (= E115) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
- R322 (≠ I251) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.
Sites not aligning to the query:
- 88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
- 128 H→A: Greatly lowers L-glutamate transport.
7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
23% identity, 82% coverage: 55:406/430 of query aligns to 71:428/452 of 7t3nA
Sites not aligning to the query:
Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
22% identity, 86% coverage: 55:423/430 of query aligns to 113:484/495 of Q8BN82
- H183 (≠ L123) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.
Sites not aligning to the query:
- 39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.
Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
21% identity, 86% coverage: 55:423/430 of query aligns to 113:484/495 of Q5Q0U0