SitesBLAST
Comparing WP_050654861.1 NCBI__GCF_002893965.1:WP_050654861.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4zz7A Crystal structure of methylmalonate-semialdehyde dehydrogenase (dddc) from oceanimonas doudoroffii (see paper)
53% identity, 96% coverage: 9:491/501 of query aligns to 4:485/489 of 4zz7A
- active site: N149 (= N155), K172 (= K178), L246 (= L252), C280 (= C286), E382 (= E386), A462 (= A466)
- binding nicotinamide-adenine-dinucleotide: T146 (≠ S152), P147 (= P153), F148 (= F154), N149 (= N155), K172 (= K178), E175 (= E181), K205 (= K211), V208 (= V214), F222 (= F228), V223 (= V229), G224 (= G230), S225 (= S231), I228 (= I234), L246 (= L252), G247 (= G253), C280 (= C286), E382 (= E386), F384 (= F388)
5tjrD X-ray crystal structure of a methylmalonate semialdehyde dehydrogenase from pseudomonas sp. Aac (see paper)
53% identity, 98% coverage: 8:497/501 of query aligns to 2:468/468 of 5tjrD
- active site: N144 (= N155), K167 (= K178), L241 (= L252), C270 (= C286), E356 (= E386), A436 (= A466)
- binding adenosine-5'-diphosphate: I140 (= I151), T141 (≠ S152), F143 (= F154), K167 (= K178), E170 (= E181), K200 (= K211), F217 (= F228), S220 (= S231), I223 (= I234)
4iymC Crystal structure of putative methylmalonate-semialdehyde dehydrogenase from sinorhizobium meliloti 1021 complexed with NAD, target 011934
54% identity, 95% coverage: 9:485/501 of query aligns to 7:485/491 of 4iymC
- active site: N153 (= N155), K176 (= K178), F250 (≠ L252), C284 (= C286), E386 (= E386), Q466 (≠ A466)
- binding nicotinamide-adenine-dinucleotide: I149 (= I151), T150 (≠ S152), P151 (= P153), F152 (= F154), N153 (= N155), F154 (= F156), K176 (= K178), K209 (= K211), V212 (= V214), F226 (= F228), V227 (= V229), G228 (= G230), S229 (= S231), I232 (= I234), G251 (= G253), C284 (= C286), E386 (= E386), F388 (= F388)
1t90A Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
45% identity, 97% coverage: 5:488/501 of query aligns to 1:482/484 of 1t90A
- active site: N151 (= N155), K174 (= K178), L248 (= L252), C282 (= C286), E380 (= E386), A460 (= A466)
- binding nicotinamide-adenine-dinucleotide: I147 (= I151), A148 (≠ S152), P149 (= P153), F150 (= F154), N151 (= N155), W159 (= W163), K174 (= K178), E177 (= E181), R178 (≠ K182), H207 (≠ K211), V225 (= V229), G226 (= G230), S227 (= S231), V230 (≠ I234), L248 (= L252), T249 (≠ G253), C282 (= C286), E380 (= E386), F382 (= F388)
P42412 Malonate-semialdehyde dehydrogenase; MSA dehydrogenase; Methylmalonate-semialdehyde dehydrogenase; MMSA dehydrogenase; MMSDH; MSDH; EC 1.2.1.27 from Bacillus subtilis (strain 168) (see 3 papers)
45% identity, 97% coverage: 5:488/501 of query aligns to 3:484/487 of P42412
- C36 (≠ G38) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
- R107 (= R109) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- A150 (≠ S152) binding NAD(+)
- F152 (= F154) binding NAD(+)
- C160 (≠ M162) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
- K176 (= K178) binding NAD(+)
- E179 (= E181) binding NAD(+)
- R180 (≠ K182) binding NAD(+)
- S229 (= S231) binding NAD(+)
- T251 (≠ G253) binding NAD(+)
- R283 (= R285) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- C287 (≠ I289) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
- C351 (≠ V352) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
- E382 (= E386) binding NAD(+)
- C413 (≠ A417) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.
8skfA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (lattice translocation disorder)
34% identity, 94% coverage: 12:481/501 of query aligns to 17:486/497 of 8skfA
- binding calcium ion: T33 (≠ V28), I34 (≠ T29), D100 (≠ E95), V187 (≠ K182)
- binding nicotinamide-adenine-dinucleotide: I156 (= I151), G157 (≠ S152), A158 (≠ P153), W159 (≠ F154), K183 (= K178), E186 (= E181), G216 (≠ K211), G220 (≠ D215), T235 (≠ V229), G236 (= G230), G237 (≠ S231), S240 (≠ I234), K243 (≠ Y237), E259 (≠ L252), C293 (= C286), F396 (= F388)
8vr1A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (ctp bound)
34% identity, 94% coverage: 12:481/501 of query aligns to 8:477/488 of 8vr1A
8vr0A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (gmp bound)
34% identity, 94% coverage: 12:481/501 of query aligns to 8:477/488 of 8vr0A
8vqzA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (cmp bound)
34% identity, 94% coverage: 12:481/501 of query aligns to 8:477/488 of 8vqzA
8vqwC Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (coa bound)
34% identity, 94% coverage: 12:481/501 of query aligns to 8:477/488 of 8vqwC