SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_051184377.1 NCBI__GCF_000422285.1:WP_051184377.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 16 hits to proteins with known functional sites (download)

P0A7B5 Glutamate 5-kinase; Gamma-glutamyl kinase; GK; EC 2.7.2.11 from Escherichia coli (strain K12) (see paper)
44% identity, 90% coverage: 24:382/398 of query aligns to 7:365/367 of P0A7B5

query
sites
P0A7B5

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S50 (= S66) binding substrate
D137 (= D153) binding substrate
N149 (= N165) binding substrate

2j5tD Glutamate 5-kinase from escherichia coli complexed with glutamate (see paper)
44% identity, 90% coverage: 24:382/398 of query aligns to 5:363/365 of 2j5tD

query
sites
2j5tD

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binding glutamic acid: S48 (= S66), G49 (= G67), A50 (= A68), N132 (= N150), D135 (= D153), D146 (= D164), N147 (= N165), A282 (= A301), A285 (= A304)

2j5vB Glutamate 5-kinase from escherichia coli complexed with glutamyl-5- phosphate and pyroglutamic acid (see paper)
40% identity, 90% coverage: 24:382/398 of query aligns to 5:323/325 of 2j5vB

query
sites
2j5vB

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binding pyroglutamic acid: T11 (≠ S30), G49 (= G67), A50 (= A68), I51 (= I69), A52 (= A70), D135 (= D153)
binding gamma-glutamyl phosphate: K8 (= K27), S48 (= S66), D135 (= D153), G145 (= G163), D146 (= D164), N147 (= N165)

2j5vA Glutamate 5-kinase from escherichia coli complexed with glutamyl-5- phosphate and pyroglutamic acid (see paper)
40% identity, 90% coverage: 24:382/398 of query aligns to 5:321/323 of 2j5vA

query
sites
2j5vA

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-

G

-

L

-

F

-

D

-

K

-

D

-

P

-

R

-

I

-

H

-

P

-

D

-

A

-

R

-

L

-

I

-

H

-

T

-

I

-

D

-

K

-

V

-

D

-

R

-

R

-

V

-

S

-

G

-

Y

-

A

-

S

-

T

-

I

-

P

-

G

-

F

-

L

-

G

-

K

-

G

-

G

Q

M

M

A

S

S

T

K

K

V

L

K

Q

A

A

A

A

Q

D

K

V

V

A

A

C

L

R

L

A

G

G

V

I

P

D

T

T

V

I

I

I

A

A

N

A

G

G

F

S

K

K

P

P

G

G

V

V

L

I

K

G

R

D

V

V

L

M

K

E

G

G

E

I

Q

S

E

V

G

G

T

T

L

L

F

F

M

H

P

A

R

Q

P

A

V

T

S

P

L

L

C

E

H

N

R

R

K

K

H

R

W

W

I

I

A

-

F

F

T

G

K

A

S

P

P

P

R

A

G

G

Q

E

I

I

V

T

V

V

D

D

N

E

G

G

A

A

E

T

R

A

A

A

L

I

L

L

E

E

N

R

G

G

K

S

S

S

L

L

L

L

P

P

S

K

G

G

I

I

K

K

E

S

V

V

V

T

G

G

R

N

F

F

S

S

L

R

G

G

D

E

A

V

V

I

L

R

I

I

V

C

S

N

E

L

E

E

G

G

E

R

D

D

L

I

A

A

V

H

G

G

M

V

V

S

N

R

Y

Y

H

N

S

S

G

D

D

A

L

L

K

R

K

R

I

I

M

A

G

G

V

H

K

H

T

S

S

Q

G

E

I

I

E

D

A

A

I

I

L

L

G

G

F

Y

K

E

H

Y

E

G

D

P

E

V

V

A

V

V

H

H

R

R

D

D

N

D

L

M

V

I

binding magnesium ion: K8 (= K27), G10 (= G29), L166 (= L184)
binding pyroglutamic acid: T11 (≠ S30), S48 (= S66), G49 (= G67), A50 (= A68), I51 (= I69)
binding gamma-glutamyl phosphate: K8 (= K27), G10 (= G29), S48 (= S66), D135 (= D153), D146 (= D164), N147 (= N165)

2akoA Crystal structure of glutamate 5-kinase from campylobacter jejuni
35% identity, 63% coverage: 22:271/398 of query aligns to 1:240/241 of 2akoA

query
sites
2akoA

R

K

R

R

I

I

V

V

V

V

K

K

V

V

G

G

S

S

G
x
H

V

V

L

I

T

S

A

E

A

E

D

N

G

T

L

L

N

S

R

F

A

E

V

R

I

L

E

K

S

N

I

L

T

V

D

A

D

F

I

L

A

A

D

K

L

L

R

M

R

E

S

-

K

K

I

Y

E

E

I

V

I

I

L

L

V

V

S

T

S

S

G

A

A

A

I

I

A

S

S

A

G

G

L

H

R

T

K

K

V

L

G

D

L

I

K

D

R

R

R

K

P

-

E

-

S

N

V

L

S

I

Q

N

Q

K

Q

Q

A

V

L

L

A

A

A

A

V

I

G

G

Q

Q

S

P

S

F

L

L

M

I

R

S

A

V

Y

Y

E

N

E

E

A

L

F

L

G

A

R

K

H

F

R

N

Q

K

K

L

V

G

A

G

Q

Q

I

I

L

L

V

L

T

T

R

G

D

K

D

D

L

F

T

D

H

S

R

R

R

K

R

A

Y

T

L

K

N

H

A

A

R

K

N

N

T

A

L

I

L

D

T

M

L

M

L

I

S

N

W

L

K

G

I

I

V

L

P

P

I

I

I

I

N

N

E

E

N

N

D

D

T

A

V

T

V

A

V

I

D

E

E

E

I

I

K

V

F

F

G

G

D

D

N

N

D

D

N

S

L

L

S

S

A

A

M

Y

I

A

T

T

C

H

L

F

S

F

E

D

A

A

G

D

L

L

L

L

V

V

N

I

L

L

T
x
S

N
x
D

L
x
I

D

D

G

G

L
x
F

F
x
Y

D

D

K

K

D
x
N

P
|
P

R

S

I

E

H

F

P

S

D

D

A

A

R

K

L

R

I

L

H

E

T

K

I

I

D

T

K

H

V

I

D

K

R

E

R

E

V

-

S

-

G

-

Y

-

A

-

S

-

T

-

I

-

P

-

G

W

F

L

L

H

G

G

K
x
T

G

G

G
|
G

M

I

A

V

S

T

K
|
K

V

L

K

K

A

A

A

A

Q

K

K

F

V

L

A

L

L

E

L

H

G

N

V

K

P

K

T

M

V

F

I

L

A

A

N

S

G

G

F

F

K

D

P

L

G

S

V

V

L

A

K

K

R

T

V

F

L

L

K

L

G

E

E

D

Q

K

E

Q

-

I

-

G

G

G

T

T

L

L

F

F

binding adenosine-5'-diphosphate: H10 (≠ G31), S161 (≠ T185), D162 (≠ N186), I163 (≠ L187), F166 (≠ L190), Y167 (≠ F191), N170 (≠ D194), P171 (= P195), T194 (≠ K227), G196 (= G229), K200 (= K233)

7wx3B Gk domain of drosophila p5cs filament with glutamate, atp, and NADPH (see paper)
36% identity, 66% coverage: 11:271/398 of query aligns to 2:256/258 of 7wx3B

query
sites
7wx3B

T

T

V

F

G

T

R

E

E

R

A

S

L

Q

L

L

R

K

G

Y

I

A

R

R

R

R

I

L

V

V

V

V

K

K

V

L

G

G

S

S

G

A

V

V

L

I

T

T

A

R

A

E

D

D

-

N

-

H

G

G

L

L

N

A

R

L

A

G

V

R

I

L

E

A

S

S

I

I

T

V

D

E

D

Q

I

V

A

A

D

E

L

C

R

H

R

L

S

E

K

G

I

R

E

E

I

V

I

M

L

M

V

V

S

T

S
|
S

G

G

A
|
A

I

V

A

A

S

F

G

G

L

K

R

Q

K

K

V

L

G

A

-

Q

-
x
E

-

L

-

L

-
x
M

-

S

L
|
L

K
x
S

R
x
M

R
|
R

P

E

E

T

S

L

V

N

S

L

Q

E

Q

P

Q

R

A

A

L

A

A

A

A

A

V

V

G

G

Q

Q

S

S

S

G

L

L

M

M

R

S

A

L

Y

Y

E

D

E

A

A

M

F

F

G

A

R

Q

H

Y

R

G

Q

V

K

K

V

I

A

A

Q

Q

I

V

L

L

V

V

T

T

R

K

D

P

D

D

L

F

T

Y

H

N

R

E

R

E

R

T

Y

R

L

N

N

N

A

L

R

F

N

C

T

T

L

L

L

S

T

E

L

L

L

I

S

S

W

L

K

N

I

I

V

V

P

P

I

I

I

I

N
|
N

E
x
T

N

N

D
|
D

T

A

V

V

V

S

V

P

D

P

E
x
M

-

F

I

I

K

P

F

I

G

K

D

D

N
|
N

D

D

N

S

L

L

S

S

A

A

M

M

I

L

T

A

C

A

L

E

S

V

E

Q

A

A

G

D

L

L

L

L

V

I

N

L

L

M

T

S

N

D

L

V

D

D

G

G

L

I

F

Y

D

N

K

K

D

P

P

P

R

W

I

-

H

E

P

D

D

G

A

A

R

K

L

L

I

M

H

H

T

T

I

Y

D

T

K

S

V

D

D

D

R

-

R

-

V

-

S

-

G

-

Y

-

A

-

S

-

T

-

I

-

P

-

G

-

F

-

L

-

G

S

K

N

G

S

G

I

M

M

A

D

S

S

K

K

V

V

K

K

A

A

A

A

Q

T

K

W

V

A

A

L

L

D

L

R

G

G

V

V

P

S

T

V

V

V

I

I

A

C

N

N

G

G

F

M

K

Q

P

E

G

K

V

A

L

I

K

K

R

T

V

I

L

I

K

G

G

G

E

R

Q

K

E

V

G

G

T

T

L

F

F

F

binding gamma-glutamyl phosphate: S59 (= S66), A61 (= A68), N149 (= N150), T150 (≠ E151), D152 (= D153), M158 (≠ E159), N165 (= N165)
binding : E72 (vs. gap), M75 (vs. gap), L77 (= L78), S78 (≠ K79), M79 (≠ R80), R80 (= R81)

7f5xA Gk domain of drosophila p5cs filament with glutamate (see paper)
35% identity, 66% coverage: 11:271/398 of query aligns to 2:234/236 of 7f5xA

query
sites
7f5xA

T

T

V

F

G

T

R

E

E

R

A

S

L

Q

L

L

R

K

G

Y

I

A

R

R

R

R

I

L

V

V

V

V

K

K

V

L

G

G

S

S

G

A

V

V

L

I

T

T

A

R

A

E

D

D

-

N

-

H

G

G

L

L

N

A

R

L

A

G

V

R

I

L

E

A

S

S

I

I

T

V

D

E

D

Q

I

V

A

A

D

E

L

C

R

H

R

L

S

E

K

G

I

R

E

E

I

V

I

M

L

M

V

V

S

T

S
|
S

G

G

A
|
A

I

V

A

A

S

F

G

G

L

K

R

Q

K

K

V

L

G

A

L

Q

K

E

R

L

R

L

P

M

E

S

S

L

V

S

S

M

Q

R

Q

P

Q

R

A

A

L

A

A

A

A

A

V

V

G

G

Q

Q

S

S

S

G

L

L

M

M

R

S

A

L

Y

Y

E

D

E

A

A

M

F

F

G

A

R

Q

H

Y

R

G

Q

V

K

K

V

I

A

A

Q

Q

I

V

L

L

V

V

T

T

R

K

D

P

D

D

L

F

T

Y

H

N

R

E

R

E

R

T

Y

R

L

N

N

N

A

L

R

F

N

C

T

T

L

L

L

S

T

E

L

L

L

I

S

S

W

L

K

N

I

I

V

V

P

P

I

I

I

I

N
|
N

E

T

N

N

D
|
D

T

A

V

V

V

S

V

-

D

-

E

-

I

-

K

-

F

-

G

P

D

D

N

N

D

D

N

S

L

L

S

S

A

A

M

M

I

L

T

A

C

A

L

E

S

V

E

Q

A

A

G

D

L

L

L

L

V

I

N

L

L

M

T

S

N

D

L

V

D

D

G

G

L

I

F

Y

D

N

K

K

D

P

P

P

R

W

I

-

H

E

P

D

D

G

A

A

R

K

L

L

I

M

H

H

T

T

I

-

D

-

K

-

V

-

D

-

R

-

R

-

V

-

S

-

G

-

Y

-

A

-

S

-

T

-

I

-

P

-

G

-

F

-

L

-

G

-

K

-

G

-

G

-

M

Y

A

T

S

S

K

K

V

V

K

K

A

A

A

A

Q

T

K

W

V

A

A

L

L

D

L

R

G

G

V

V

P

S

T

V

V

V

I

I

A

C

N

N

G

G

F

M

K

Q

P

E

G

K

V

A

L

I

K

K

R

T

V

I

L

I

K

G

G

G

E

R

Q

K

E

V

G

G

T

T

L

F

F

F

binding gamma-l-glutamic acid: S59 (= S66), A61 (= A68), N143 (= N150), D146 (= D153)

8j0gB Gk monomer complexes with glutamate and atp
35% identity, 64% coverage: 16:271/398 of query aligns to 2:267/274 of 8j0gB

query
sites
8j0gB

A

A

L

F

L

A

R

K

G

D

I

V

R

K

R

R

I

I

V

V

V

V

K
|
K

V

V

G
|
G

S
x
T

G
x
A

V

V

L

V

T

T

A

G

A

K

D

G

G

G

-

R

L

L

N

A

R

L

A

G

V

R

I

L

E

G

S

A

I

I

T

C

D

E

D

Q

I

L

A

A

D

E

L

L

R

N

R

S

S

D

K

G

I

F

E

E

I

V

I

I

L

L

V

V

S

S

S

S

G

G

A
|
A

I

V

A

G

S

L

G

G

L

R

R

Q

K

R

V

L

G

R

L
x
Y

K

R

R

Q

-
x
L

-
x
V

-

N

-

S

-

S

-
x
F

-

A

-

D

-

L

-

Q

R

K

P

P

E

Q

S

M

V

E

S

L

Q

D

Q

G

Q

K

A

A

L

C

A

A

A

G

V

V

G

G

Q

Q

S

S

S

S

L

L

M

M

R

A

A

Y

Y

Y

E

E

E

T

A

M

F

F

G

D

R

Q

H

L

R

D

Q

V

K

T

V

V

A

A

Q

Q

I

M

L

L

V

V

T

T

R

D

D

S

D

S

L

F

T

R

H

D

R

K

R

D

R

F

Y

R

L

K

N

Q

A

L

R

S

N

E

T

T

L

V

L

K

T

A

L

M

L

L

S

R

W

M

K

R

I

V

V

I

P

P

I

V

I

F

N
|
N

E

E

N

N

D
|
D

T

A

V

I

V

S

V

T

D

R

E

R

I

T

K

G

-

I

F

F

G

W

D

D

N
|
N

D

D

N

S

L

L

S

A

A

A

M

L

I

L

T

S

C

L

L

E

S

L

E

K

A

A

G

D

L

L

L

L

V

I

N

L

L

L

T

S

N

D

L
x
V

D

E

G

G

L

L

F

Y

D

T

K
x
G

D

P

P
|
P

R

S

I

-

H

D

P

S

D

T

A

S

R

K

L

L

I

I

H

H

T

T

I

F

D

I

K

K

-

E

-

K

-

H

V

Q

D

D

R

E

R

I

V

T

S
x
F

G

G

Y

E

A

K

S

S

T

K

I

-

P

-

G

-

F

-

L

L

G

G

K
x
R

G
|
G

G
|
G

M
|
M

A

T

S

A

K
|
K

V

V

K

K

A

A

A

A

Q

V

K

N

V

A

A

A

L

Y

L

G

G

G

V

V

P

P

T

V

V

I

I

I

A

T

N

S

G

G

F

Y

K

A

P

A

G

E

V

N

L

I

K

S

R

K

V

V

L

L

K

R

G

G

E

L

Q

R

E

V

G

G

T

T

L

L

F

F

binding adenosine-5'-triphosphate: K13 (= K27), G15 (= G29), T16 (≠ S30), A17 (≠ G31), V185 (≠ L187), G191 (≠ K193), P193 (= P195), F215 (≠ S215), R223 (≠ K227), G225 (= G229), M226 (= M230), K229 (= K233)
binding gamma-l-glutamic acid: A55 (= A68), N147 (= N150), D150 (= D153), N163 (= N165), R223 (≠ K227)
binding magnesium ion: R223 (≠ K227), G224 (= G228), G225 (= G229)
binding : Y65 (≠ L78), L68 (vs. gap), V69 (vs. gap), F73 (vs. gap)

8j0eB Gk monomer complexes with catalytic intermediate
34% identity, 64% coverage: 16:271/398 of query aligns to 5:262/269 of 8j0eB

query
sites
8j0eB

A

A

L

F

L

A

R

K

G

D

I

V

R

K

R

R

I

I

V

V

V

V

K
|
K

V

V

G
|
G

S
x
T

G
x
A

V

V

L

V

T

T

A

G

A

K

D

G

G

G

-

R

L

L

N

A

R

L

A

G

V

R

I

L

E

G

S

A

I

I

T

C

D

E

D

Q

I

L

A

A

D

E

L

L

R

N

R

S

S

D

K

G

I

F

E

E

I

V

I

I

L

L

V

V

S

S

S
|
S

G
|
G

A

A

I
x
V

A

G

S

L

G

G

L

R

R

Q

K

R

V

L

G

R

L
x
Y

K

R

R

Q

-
x
L

-
x
V

-

N

-
x
S

-

S

-
x
F

-

A

-

D

-

L

-
x
Q

R

K

P

P

E

Q

S

M

V

E

S

L

Q

D

Q

G

Q

K

A

A

L

C

A

A

A

G

V

V

G

G

Q

Q

S

S

S

S

L

L

M

M

R

A

A

Y

Y

Y

E

E

E

T

A

M

F

F

G

D

R

Q

H

L

R

D

Q

V

K

T

V

V

A

A

Q

Q

I

M

L

L

V

V

T

T

R

D

D

S

D

S

L

F

T

R

H

D

R

K

R

D

R

F

Y

R

L

K

N

Q

A

L

R

S

N

E

T

T

L

V

L

K

T

A

L

M

L

L

S

R

W

M

K

R

I

V

V

I

P

P

I

V

I

F

N

N

E

E

N

N

D
|
D

T

A

V

I

V

-

V

-

D

-

E

S

I

T

K

R

F

F

G

W

D

D

N
|
N

D
|
D

N

S

L

L

S

A

A

A

M

L

I

L

T

S

C

L

L

E

S

L

E

K

A

A

G

D

L

L

L

L

V

I

N

L

L

L

T

S

N

D

L
x
V

D

E

G

G

L

L

F

Y

D

T

K

G

D

P

P
|
P

R

S

I

-

H

D

P

S

D

T

A

S

R

K

L

L

I

I

H

H

T

T

I

F

D

I

K

K

V

-

D

-

R

-

R

-

V

-

S

E

G

K

Y

H

A

Q

S

D

T

E

I

I

P

T

G

F

F

G

L

E

G
x
K

K
x
R

G
|
G

G
|
G

M
|
M

A

T

S

A

K

K

V

V

K

K

A

A

A

A

Q

V

K

N

V

A

A

A

L

Y

L

G

G

G

V

V

P

P

T

V

V

I

I

I

A

T

N

S

G

G

F

Y

K

A

P

A

G

E

V

N

L

I

K

S

R

K

V

V

L

L

K

R

G

G

E

L

Q

R

E

V

G

G

T

T

L

L

F

F

binding magnesium ion: D163 (= D166), G219 (= G228)
binding (2~{R})-5-[[[[(2~{R},3~{S},4~{S},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-2-azanyl-5-oxidanylidene-pentanoic acid: K16 (= K27), G18 (= G29), T19 (≠ S30), A20 (≠ G31), S56 (= S66), G57 (= G67), V59 (≠ I69), D153 (= D153), N162 (= N165), V184 (≠ L187), P192 (= P195), K217 (≠ G226), R218 (≠ K227), G219 (= G228), G220 (= G229), M221 (= M230)
binding : Y68 (≠ L78), L71 (vs. gap), V72 (vs. gap), V72 (vs. gap), S74 (vs. gap), F76 (vs. gap), F76 (vs. gap), Q80 (vs. gap)

8j0fA Gk tetramer with adjacent hooks at reaction state (see paper)
34% identity, 64% coverage: 16:271/398 of query aligns to 6:261/270 of 8j0fA

query
sites
8j0fA

A

A

L

F

L

A

R

K

G

D

I

V

R

K

R

R

I

I

V

V

V

V

K
|
K

V

V

G

G

S
x
T

G
x
A

V

V

L

V

T

T

A

G

A

K

D

G

G

G

-

R

L

L

N

A

R

L

A

G

V

R

I

L

E

G

S

A

I

I

T

C

D

E

D

Q

I

L

A

A

D

E

L

L

R

N

R

S

S

D

K

G

I

F

E

E

I

V

I

I

L

L

V

V

S

S

S
|
S

G

G

A

A

I

V

A

G

S

L

G

G

L

R

R

Q

K

R

V

L

G

R

L

Y

K

R

R

Q

-
x
L

-
x
V

-
x
N

-
x
S

-
x
S

-
x
F

-
x
A

-

D

-
x
L

-

Q

R

K

P

P

E

Q

S

M

V

E

S

L

Q

D

Q

G

Q

K

A

A

L

C

A

A

A

G

V

V

G

G

Q

Q

S

S

S

S

L

L

M

M

R

A

A

Y

Y

Y

E

E

E

T

A

M

F

F

G

D

R

Q

H

L

R

D

Q

V

K

T

V

V

A

A

Q

Q

I

M

L

L

V

V

T

T

R

D

D

S

D

S

L

F

T

R

H

D

R

K

R

D

R

F

Y

R

L

K

N

Q

A

L

R

S

N

E

T

T

L

V

L

K

T

A

L

M

L

L

S

R

W

M

K

R

I

V

V

I

P

P

I

V

I

F

N

N

E

E

N

N

D
|
D

T

A

V

I

V

S

V

T

D

R

E

-

I

-

K

-

F

-

G

W

D

D

N
|
N

D

D

N

S

L

L

S

A

A

A

M

L

I

L

T

S

C

L

L

E

S

L

E

K

A

A

G

D

L

L

L

L

V

I

N

L

L

L

T

S

N
x
D

L
x
V

D

E

G

G

L

L

F
x
Y

D

T

K
x
G

D

P

P

P

R

S

I

-

H

D

P

S

D

T

A

S

R

K

L

L

I

I

H

H

T

T

I

F

D

I

K

K

V

-

D

-

R

-

R

-

V

-

S

E

G

K

Y

H

A

Q

S

D

T

E

I

I

P

T

G

-

F
|
F

L

G

G

E

K
x
R

G
|
G

G
|
G

M
|
M

A

T

S

A

K

K

V

V

K

K

A

A

A

A

Q

V

K

N

V

A

A

A

L

Y

L

G

G

G

V

V

P

P

T

V

V

I

I

I

A

T

N

S

G

G

F

Y

K

A

P

A

G

E

V

N

L

I

K

S

R

K

V

V

L

L

K

R

G

G

E

L

Q

R

E

V

G

G

T

T

L

L

F

F

binding adenosine-5'-diphosphate: A21 (≠ G31), D183 (≠ N186), V184 (≠ L187), Y188 (≠ F191), G190 (≠ K193), F214 (= F224), G219 (= G229), M220 (= M230)
binding magnesium ion: R217 (≠ K227), G218 (= G228), G219 (= G229)
binding gamma-glutamyl phosphate: K17 (= K27), T20 (≠ S30), S57 (= S66), D154 (= D153), N162 (= N165), R217 (≠ K227)
binding : L72 (vs. gap), V73 (vs. gap), N74 (vs. gap), S75 (vs. gap), S76 (vs. gap), F77 (vs. gap), F77 (vs. gap), A78 (vs. gap), L80 (vs. gap)

2bndA The structure of e. Coli ump kinase in complex with udp (see paper)
24% identity, 63% coverage: 22:270/398 of query aligns to 6:234/237 of 2bndA

query
sites
2bndA

R

K

R

R

I

I

V

L

V

L

K

K

V

L

G
x
S

S
x
G

G

E

V

A

L

L

-

Q

-

G

T

T

A

E

A

G

D

F

G

G

L

I

N

D

R

A

A

S

V

I

I

L

E

D

S

R

I

M

T

A

D

Q

D

E

I

I

A

K

D

E

L

L

R

V

R

E

S

L

K

G

I

I

E

Q

I

V

-

G

I

V

L

V

V

I

S
x
G

S
x
G

G
|
G

A

N

I

L

-

F

-
x
R

A
x
G

S

A

G

G

L

L

R

A

K

K

V

A

G

G

L

M

K

N

R

R

R

-

P

-

E

-

S

-

V

-

S

-

Q

-

Q

-

Q

-

A

-

L

-

A

-

A

-

V

-

G

-

Q

-

S

-

S

-

L

-

M

-

R

-

A

-

Y

-

E

-

E

-

A

V

F

V

G

G

R
x
D

H

H

R

M

Q
x
G

K
x
M

V

L

A

A

Q

T

I

V

-

M

-

N

-

G

L

L

V

A

T

M

R

R

D

D

D

A

L

L

T

-

H

-

R

H

R

R

R

A

Y

Y

L

V

N

N

A

A

R

R

-

L

-

M

-

S

-

A

-

I

-

P

-

L

N

N

T

A

L

V

L

C

T

D

L

S

L

Y

S

S

W

W

K

A

I

E

V

A

P

I

I

S

I

L

N

L

E

R

N

N

D

N

T

R

V

V

V

V

V

I

D

L

E

S

I

A

K

G

F
x
T

G

G

D
x
N

N

P

D

F

N
x
F

L
x
T

S
x
T

A

D

M

S

I

A

T

A

C

C

L

L

S

R

-

G

-

I

-

E

-

I

E

E

A

A

G

N

L

V

L

V

V

L

N

K

L

A

T

T

N

K

L

V

D

D

G

G

L

V

F

F

D

T

K

A

D

D

P

P

R

A

I

K

H

D

P

P

D

T

A

A

R

T

L

M

I

Y

H

E

T

Q

I

L

D

T

K

-

V

-

D

-

R

-

R

-

V

-

S

-

G

-

Y

Y

A

S

S

E

T

V

I

L

P

E

G

K

F

E

L

L

G

K

K

V

G

M

G

D

M

L

A

A

S

A

K

F

V

T

K

L

A

A

A

R

Q

D

K

H

V

-

A

-

L

-

L

-

G

K

V

L

P

P

T

I

V

R

I

V

A

F

N

N

G

M

F

N

K

K

P

P

G

G

V

A

L

L

K

R

R

R

V

V

L

V

K

M

G

G

E

E

Q

K

E

E

G

G

T

T

L

L

binding uridine-5'-diphosphate: S13 (≠ G29), G14 (≠ S30), G52 (≠ S65), G53 (≠ S66), G54 (= G67), R58 (vs. gap), G59 (≠ A70), D73 (≠ R109), G76 (≠ Q112), M77 (≠ K113), T134 (≠ F162), N136 (≠ D164), F139 (≠ N167), T140 (≠ L168), T141 (≠ S169)

2bneB The structure of e. Coli ump kinase in complex with ump (see paper)
24% identity, 63% coverage: 22:270/398 of query aligns to 8:235/238 of 2bneB

query
sites
2bneB

R

K

R

R

I

I

V

L

V

L

K

K

V

L

G

S

S

G

G

E

V

A

L

L

T

Q

A

G

A

T

D

E

-

F

G

G

L

I

N

D

R

A

A

S

V

I

I

L

E

D

S

R

I

M

T

A

D

Q

D

E

I

I

A

K

D

E

L

L

R

V

R

E

S

L

K

G

I

I

E

Q

I

V

-

G

I

V

L

V

V

I

S
x
G

S
x
G

G
|
G

A

N

I

L

-

F

-
x
R

A
x
G

S

A

G

G

L

L

R

A

K

K

V

A

G

G

L

M

K

N

R

R

R

-

P

-

E

-

S

-

V

-

S

-

Q

-

Q

-

Q

-

A

-

L

-

A

-

A

-

V

-

G

-

Q

-

S

-

S

-

L

-

M

-

R

-

A

-

Y

-

E

-

E

-

A

V

F

V

G

G

R
x
D

H

H

R

M

Q
x
G

K
x
M

V

L

A

A

Q

T

I

V

-

M

-

N

-

G

L

L

V

A

T

M

R

R

D

D

D

A

L

L

T

-

H

-

R

H

R

R

R

A

Y

Y

L

V

N

N

A

A

R

R

-

L

-

M

-

S

-

A

-

I

-

P

-

L

N

N

T

G

L

V

L

C

T

D

L

S

L

Y

S

S

W

W

K

A

I

E

V

A

P

I

I

S

I

L

N

L

E

R

N

N

D

N

T

R

V

V

V

V

V

I

D

L

E

S

I

A

K

G

F
x
T

G

G

D
x
N

N

P

D

F

N
x
F

L
x
T

S
x
T

A

D

M

S

I

A

T

A

C

C

L

L

S

R

-

G

-

I

-

E

-

I

E

E

A

A

G

N

L

V

L

V

V

L

N

K

L

A

T

T

N

K

L

V

D

D

G

G

L

V

F

F

D

T

K

A

D

D

P

P

R

A

I

K

H

D

P

P

D

T

A

A

R

T

L

M

I

Y

H

E

T

Q

I

L

D

T

K

-

V

-

D

-

R

-

R

-

V

-

S

-

G

-

Y

Y

A

S

S

E

T

V

I

L

P

E

G

K

F

E

L

L

G

K

K

V

G

M

G

D

M

L

A

A

S

A

K

F

V

T

K

L

A

A

A

R

Q

D

K

H

V

-

A

-

L

-

L

-

G

K

V

L

P

P

T

I

V

R

I

V

A

F

N

N

G

M

F

N

K

K

P

P

G

G

V

A

L

L

K

R

R

R

V

V

L

V

K

M

G

G

E

E

Q

K

E

E

G

G

T

T

L

L

binding uridine-5'-monophosphate: G53 (≠ S65), G54 (≠ S66), G55 (= G67), R59 (vs. gap), G60 (≠ A70), D74 (≠ R109), G77 (≠ Q112), M78 (≠ K113), T135 (≠ F162), N137 (≠ D164), F140 (≠ N167), T141 (≠ L168), T142 (≠ S169)

2v4yA The structure of e. Coli ump kinase in complex with its allosteric regulator gtp (see paper)
24% identity, 63% coverage: 22:270/398 of query aligns to 6:234/237 of 2v4yA

query
sites
2v4yA

R

K

R

R

I

I

V

L

V

L

K

K

V

L

G
x
S

S
x
G

G
x
E

V

A

L

L

-

Q

-

G

T

T

A

E

A

G

D

F

G

G

L

I

N

D

R

A

A

S

V

I

I

L

E

D

S

R

I

M

T

A

D

Q

D

E

I

I

A

K

D

E

L

L

R

V

R

E

S

L

K

G

I

I

E

Q

I

V

-

G

I

V

L

V

V

I

S
x
G

S
x
G

G
|
G

A

N

I

L

-

F

-

R

A

G

S

A

G

G

L

L

R

A

K

K

V

A

G

G

L

M

K

N

R

R

R

-

P

-

E

-

S

-

V

-

S

-

Q

-

Q

-

Q

-

A

-

L

-

A

-

A

-

V

-

G

-

Q

-

S

-

S

-

L

-

M

-

R

-

A

-

Y

-

E

-

E

-

A

V

F

V

G

G

R

D

H

H

R

M

Q

G

K

M

V

L

A

A

Q

T

I

V

-

M

-

N

-

G

L

L

V

A

T

M

R

R

D
|
D

D

A

L

L

T

-

H

-

R
x
H

R

R

R

A

Y

Y

L

V

N
|
N

A
|
A

R
|
R

-

L

-

M

-

S

-

A

-

I

-

P

-

L

N

N

T

G

L

V

L

C

T

D

L

S

L

Y

S

S

W
|
W

K
x
A

I

E

V

A

P

I

I
x
S

I

L

N

L

E
x
R

N

N

D

N

T
x
R

V

V

V

V

V

I

D

L

E

S

I

A

K

G

F

T

G

G

D

N

N

P

D

F

N

F

L
x
T

S

T

A

D

M

S

I

A

T

A

C

C

L

L

S

R

-

G

-

I

-

E

-

I

E

E

A

A

G

N

L

V

L

V

V

L

N

K

L

A

T

T

N

K

L

V

D

D

G

G

L

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F

F

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T

K

A

D

D

P

P

R

A

I

K

H

D

P

P

D

T

A

A

R

T

L

M

I

Y

H

E

T

Q

I

L

D

T

K

-

V

-

D

-

R

-

R

-

V

-

S

-

G

-

Y

Y

A

S

S

E

T

V

I

L

P

E

G

K

F

E

L

L

G

K

K

V

G

M

G

D

M

L

A

A

S

A

K

F

V

T

K

L

A

A

A

R

Q

D

K

H

V

-

A

-

L

-

L

-

G

K

V

L

P

P

T

I

V

R

I

V

A

F

N

N

G

M

F

N

K

K

P

P

G

G

V

A

L

L

K

R

R

R

V

V

L

V

K

M

G

G

E

E

Q

K

E

E

G

G

T

T

L

L

binding guanosine-5'-triphosphate: S13 (≠ G29), G14 (≠ S30), E15 (≠ G31), G52 (≠ S65), G53 (≠ S66), G54 (= G67), D89 (= D122), H92 (≠ R127), N97 (= N132), A98 (= A133), R99 (= R134), W115 (= W143), A116 (≠ K144), S120 (≠ I148), R123 (≠ E151), R126 (≠ T154), T140 (≠ L168)

P0A7E9 Uridylate kinase; UK; Uridine monophosphate kinase; UMP kinase; UMPK; EC 2.7.4.22 from Escherichia coli (strain K12) (see 4 papers)
24% identity, 63% coverage: 22:270/398 of query aligns to 10:238/241 of P0A7E9

query
sites
P0A7E9

R

K

R

R

I

I

V

L

V

L

K
|
K

V
x
L

G
x
S

S
x
G

G

E

V

A

L

L

-

Q

-

G

T

T

A

E

A

G

D

F

G

G

L

I

N

D

R

A

A

S

V

I

I

L

E

D

S

R

I

M

T

A

D

Q

D

E

I

I

A

K

D

E

L

L

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V

R

E

S

L

K

G

I

I

E

Q

I

V

-

G

I

V

L

V

V

I

S

G

S
x
G

G
|
G

A

N

I

L

-

F

-
x
R

A

G

S

A

G

G

L

L

R

A

K

K

V

A

G

G

L

M

K

N

R

R

R

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P

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E

-

S

-

V

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S

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Q

-

Q

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Q

-

A

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L

-

A

-

A

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V

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G

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Q

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S

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S

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L

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M

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R

-

A

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Y

-

E

-

E

-

A

V

F

V

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G

R
x
D

H

H

R

M

Q

G

K

M

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L

A

A

Q

T

I

V

-

M

-

N

-

G

L

L

V

A

T

M

R
|
R

D
|
D

D
x
A

L
|
L

T

-

H

-

R
x
H

R

R

R

A

Y

Y

L

V

N
|
N

A
|
A

R
|
R

-

L

-

M

-

S

-

A

-

I

-

P

-

L

N

N

T

G

L

V

L

C

T

D

L

S

L

Y

S

S

W

W

K

A

I

E

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A

P

I

I

S

I

L

N

L

E

R

N

N

D

N

T

R

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V

V

V

V

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D

L

E

S

I

A

K

G

F
x
T

G
|
G

D
x
N

N
x
P

D
x
F

N
x
F

L
x
T

S
x
T

A
x
D

M

S

I

A

T

A

C

C

L

L

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R

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G

-

I

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E

-

I

E

E

A

A

G
x
D

L

V

L

V

V

L

N

K

L

A

T

T

N

K

L

V

D

D

G

G

L

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F

D

T

K

A

D
|
D

P

P

R

A

I

K

H

D

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P

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T

A

A

R

T

L

M

I

Y

H

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T

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I

L

D

T

K

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V

-

D

-

R

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R

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G

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Y

Y

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E

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F

E

L

L

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K

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G
x
D

M

L

A

A

S

A

K

F

V

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K

L

A

A

A

R

Q

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K

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A

-

L

-

L

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G

K

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L

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P

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R

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F

N

N

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M

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N

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K

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P

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R

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L

KLSG 15:18 (≠ KVGS 27:30) binding ATP
G57 (≠ S66) binding UMP
G58 (= G67) binding ATP
R62 (vs. gap) binding ATP; mutation to H: Loss of activation by GTP and decreased affinity for UTP.
D77 (≠ R109) binding UMP; mutation to N: Loss of activation by GTP and decreased affinity for UTP.
RDAL--H 92:96 (≠ RDDLTHR 121:127) binding ATP
D93 (= D122) mutation to A: Loss of activation by GTP and of inhibition by UTP.
NAR 101:103 (= NAR 132:134) binding ATP
T138 (≠ F162) mutation to A: Loss of activation by GTP. Moderate loss of sensitivity to UTP inhibition. 4-fold and 2-fold decrease in affinity for UMP and ATP, respectively.
138:145 (vs. 162:169, 13% identical) binding UMP
N140 (≠ D164) mutation to A: Loss of activation by GTP. Moderate loss of sensitivity to UTP inhibition.
D146 (≠ A170) mutation to N: Drastically reduced activity.
D159 (≠ G179) mutation to N: Increased solubility at neutral pH. Nearly no change in kinetic properties and stability.
D174 (= D194) mutation to N: Reduced UMP-binding affinity.
D201 (≠ G229) mutation to N: Loss of activation by GTP.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2bnfA The structure of e. Coli ump kinase in complex with utp (see paper)
24% identity, 63% coverage: 22:270/398 of query aligns to 6:233/236 of 2bnfA

query
sites
2bnfA

R

K

R

R

I

I

V

L

V

L

K
|
K

V

L

G
x
S

S
x
G

G

E

V

A

L

L

-

Q

-

G

T

T

A

E

A

G

D

F

G

G

L

I

N

D

R

A

A

S

V

I

I

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E

D

S

R

I

M

T

A

D

Q

D

E

I

I

A

K

D

E

L

L

R

V

R

E

S

L

K

G

I

I

E

Q

I

V

-

G

I

V

L

V

V

I

S
x
G

S
x
G

G
|
G

A

N

I

L

-
x
F

-
x
R

A
x
G

S

A

G

G

L

L

R

A

K

K

V

A

G

G

L

M

K

N

R

R

R

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P

-

E

-

S

-

V

-

S

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Q

-

Q

-

Q

-

A

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L

-

A

-

A

-

V

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G

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Q

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S

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S

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L

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M

-

R

-

A

-

Y

-

E

-

E

-

A

V

F

V

G

G

R
x
D

H

H

R

M

Q
x
G

K
x
M

V

L

A

A

Q

T

I

V

-

M

-

N

-

G

L

L

V

A

T

M

R

R

D

D

D

A

L

L

T

-

H

-

R

H

R

R

R

A

Y

Y

L

V

N

N

A

A

R

R

N

-

T

-

L

L

L

M

T

S

L

A

L

I

-

P

-

L

-

N

-

V

-

C

-

D

-

S

-

Y

S

S

W

W

K

A

I

E

V

A

P

I

I

S

I

L

N

L

E

R

N

N

D

N

T

R

V

V

V

V

V

I

D

L

E

S

I

A

K

G

F
x
T

G

G

D
x
N

N

P

D

F

N
x
F

L
x
T

S
x
T

A

D

M

S

I

A

T

A

C

C

L

L

S

R

-

G

-

I

-

E

-

I

E

E

A

A

G

N

L

V

L

V

V

L

N

K

L

A

T

T

N

K

L

V

D

D

G

G

L

V

F

F

D

T

K

A

D

D

P

P

R

A

I

K

H

D

P

P

D

T

A

A

R

T

L

M

I

Y

H

E

T

Q

I

L

D

T

K

-

V

-

D

-

R

-

R

-

V

-

S

-

G

-

Y

Y

A

S

S

E

T

V

I

L

P

E

G

K

F

E

L

L

G

K

K

V

G

M

G

D

M

L

A

A

S

A

K

F

V

T

K

L

A

A

A

R

Q

D

K

H

V

-

A

-

L

-

L

-

G

K

V

L

P

P

T

I

V

R

I

V

A

F

N

N

G

M

F

N

K

K

P

P

G

G

V

A

L

L

K

R

R

R

V

V

L

V

K

M

G

G

E

E

Q

K

E

E

G

G

T

T

L

L

binding uridine 5'-triphosphate: K11 (= K27), S13 (≠ G29), G14 (≠ S30), G52 (≠ S65), G53 (≠ S66), G54 (= G67), F57 (vs. gap), R58 (vs. gap), G59 (≠ A70), D73 (≠ R109), G76 (≠ Q112), M77 (≠ K113), T133 (≠ F162), N135 (≠ D164), F138 (≠ N167), T139 (≠ L168), T140 (≠ S169)

3ll9B X-ray structures of isopentenyl phosphate kinase (see paper)
32% identity, 21% coverage: 185:266/398 of query aligns to 178:242/249 of 3ll9B

query
sites
3ll9B

T
|
T

N
x
D

L
x
V

D

D

G

G

L

V

F
x
Y

D

T

K
x
R

D
x
N

P
|
P

R
x
K

I

K

H

H

P

P

D

D

A

A

R

R

L

L

I

L

H

D

T

V