SitesBLAST

P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
22% identity, 98% coverage: 3:421/427 of query aligns to 79:502/534 of P53322

query
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P53322

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K283 (≠ Q208) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
22% identity, 95% coverage: 10:414/427 of query aligns to 4:387/393 of 6e9oA

query
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6e9oA

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binding D-galactonic acid: Y21 (≠ F28), F114 (= F121), Q141 (≠ A148), Q228 (≠ V257), S320 (≠ A343), N343 (= N369)

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
23% identity, 87% coverage: 57:426/427 of query aligns to 113:489/495 of Q9NRA2

query
sites
Q9NRA2

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D

K136 (≠ R80) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ I125) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ IM 140:141) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ LQ 214:215) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ KTLAA 216:220) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G273) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (≠ V279) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (= G320) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

8u3gA Structure of naag-bound sialin (see paper)
23% identity, 86% coverage: 57:425/427 of query aligns to 52:427/427 of 8u3gA

query
sites
8u3gA

G

G

I

S

F

F

V

Y

A

G

Y

Y

A

I

F

I

L

T

G

Q

V

I

P

P

A

G

N

G

L

Y

L

V

M

A

R

S

K

K

F

I

G

G

A

G

R

K

A

M

W

L

I

L

G

G

S

F

T

G

T

I

L

L

V

G

W

T

G

A

V

V

L

L

S

T

-

L

-

F

-

T

-

P

-

I

-

A

A

A

S

D

M

L

G

G

Y

V

A

G

D

-

T

-

E

-

W

-

K

P

F

L

L

I

L

V

V

L

R

R

S

A

L

L

L

E

G

G

A

L

A

G

E

E

A

G

G

V

F

T

F
|
F

P

P

G

A

M

M

I

H

Y

A

L

M

T

W

S

S

Q

S

W

W

F

A

P

P

Q

P

K

L

S

E

R

R

A

S

G

K

I

L

M

L

G

S

L

I

F

S

Y

Y

M

A

G

G

A

A

P

Q

L

L

A

G

L

T

T

V

L

I

G

S

S

L

P

P

L

L

S

S

G

G

A

I

L

I

L

C

E

Y

M

-

H

-

G

-

F

-

M

-

G

-

H

Y

P

M

G

N

W

W

Y

T

W

Y

M

V

F

F

I

Y

I

F

E

F

G

G

A

T

L

I

A

G

L

I

V

F

A

W

G

F

V

L

A

L

T

W

F

I

C

W

Y

L

L

V

D

S

D

D

R

T

P

P

S

Q

E

K

A

H

R

K

F

R

L

I

S

S

Q

H

V

Y

E

E

R

K

D

E

L

Y

L

I

Q

L

K

S

T

S

L

L

A

R

A

N

E

Q

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L

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S

K

Q

S

K

T

S

S

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K

P

I

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S

V

D

P

A

I

A

L

R

K

N

S

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L

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P

V

L

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W

H

A

L

I

A

V

I

V

I

A

Y

H

M

F

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Y

Q

N

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Y

Y

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T

Y

L

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L

P

P

S

T

Q

Y

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M

A

K

A

E

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I

M

L

G

R

T

F

N

N

V

V

-

Q

-

E

-

N

G

G

F

F

T

L

A

S

S

S

L

L

V

P

T

Y

A

L

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G

P

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W

W

V

L

A

C

A

M

L

I

F

L

G

S

T

G

Y

-

I

-

P

-

R

Q

Y

A

S

A

D

D

R

N

T

L

G

R

E

A

R

K

R

W

N

N

I

F

A

S

A

T

L

L

T

C

L

V

L

R

V

R

A

I

G

F

L

S

G

L

I

I

G

G

I

M

S

-

A

-

F

-

A

I

S

G

P

P

V

A

V

V

A

F

I

L

V

V

A

A

L

A

C

G

F

F

A

I

A

G

A

C

G

D

F

Y

I

-

A

S

V

L

Q

A

P

V

V

A

F

F

W

L

T

T

M

I

P

S

T

T

G

T

L

L

-

G

-

F

-

C

L

S

S

S

G

G

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F

A
x
S

L

I

A

N

-
x
H

-

L

-

D

-

I

-

A

-

P

-

S

-

Y

A

A

G

G

I

I

-

L

-
x
L

G

G

F

I

T

T

N

N

M

T

F

F

G

A

A

T

F

I

G

P

G

G

F

M

L

V

A

G

P

P

N

V

I

I

K

A

A

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Q

S

-

L

-

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A

P

D

D

L

N

F

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F

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G

N

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H

W

L

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A

T

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V

L

F

V

Y

S

I

L

A

A

A

I

A

I

I

T

N

V

V

I

F

G

G

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A

I

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T

M

L

L

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A

K

K

K

G

E

E

A

V

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Q

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N

V

W

V

A

L

L

S

N

binding aspartic acid: F118 (= F121)
binding glutamic acid: S350 (≠ A360), H353 (vs. gap), L365 (vs. gap)

Sites not aligning to the query:

binding aspartic acid: 15, 19

8u3hA Structure of fmoc-leu-oh bound sialin (see paper)
23% identity, 86% coverage: 57:425/427 of query aligns to 50:425/425 of 8u3hA

query
sites
8u3hA

G

G

I

S

F

F

V

Y

A

G

Y

Y

A

I

F

I

L

T

G

Q

V

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P

A

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N

G

L

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L

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M

A

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K

F

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G

A

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F

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I

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L

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G

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T

G

A

V

V

L

L

S

T

-

L

-

F

-

T

-

P

-

I

-

A

A

A

S

D

M

L

G

G

Y

V

A

G

D

-

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-

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P

F

L

L

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L

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V

L

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R

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A

L

L

L

E

G

G

A

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A

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A

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A

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A

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G

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Q

L

L

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L

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S

L

P

P

L

L

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G

A

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M

-

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M

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Y

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M

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N

W

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Y

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W

Y

M

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F

F

I

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I

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x
H

M

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x
Y

Q

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Y
|
Y

G

T

L

L

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T

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Y

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A

K

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N

N

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-

Q

-

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-

N

G

G

F

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T

L

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S

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L

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Y

A

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W

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x
M

L

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Y

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Y

A

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A

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D

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N

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L

G

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A

R

K

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N

N

I

F

A

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A

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L

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L

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V

R

A

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F

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L

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I

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G

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M

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-

A

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-

A

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P

V

A

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V

A

F

I

L

V

V

A

A

L

A

C

G

F

F

A

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A

G

A

C

G

D

F

Y

I

-

A

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M

I

P

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T

T

G

T

L

L

-

G

-

F

-

C

L
x
S

S

S

G

G

T

F

A

S

L

I

A

N

-

H

-

L

-

D

-

I

-

A

-

P

-

S

-

Y

A

A

G

G

I

I

-

L

G

G

F

I

T

T

N

N

M

T

F

F

G

A

A

T

F

I

G

P

G

G

F

M

L

V

A

G

P

P

N

V

I

I

K

A

A

K

Q

S

-

L

-

T

A

P

D

D

L

N

F

T

F

V

G

G

N

E

H

W

L

Q

A

T

G

V

L

F

V

Y

S

I

L

A

A

A

I

A

I

I

T

N

V

V

I

F

G

G

S

A

I

I

T

F

I

F

M

T

M

L

L

F

P

A

K

K

K

G

E

E

A

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P

Q

R

N

V

W

V

A

L

L

S

N

binding leucine: M279 (≠ A287), S344 (≠ L356)
binding Fluorenylmethyloxycarbonyl chloride: H235 (≠ Y246), Y238 (≠ I249), Y243 (= Y254)

Sites not aligning to the query:

binding Fluorenylmethyloxycarbonyl chloride: 19

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 75% coverage: 107:426/427 of query aligns to 165:489/495 of Q5Q0U0

query
sites
Q5Q0U0

L

I

L

V

V

L

R
|
R

S

A

L

L

L
x
E

G
|
G

A

L

A

G

E
|
E

A
x
G

G

V

F

T

F
|
F

P
|
P

G

A

M

M

I
x
H

Y

A

L

M

T
x
W

S

S

Q

S

W

W

F

A

P

P

Q

P

K

L

S

E

R

R

A

S

G

K

I

L

M

L

G

T

L

I

F

S

Y

Y

M

A

G

G

A

A

P

Q

L

L

A

G

L

T

T

V

L

I

G

S

S

L

P

P

L

L

S

S

G

G

A

I

L

I

L

C

E

Y

M

-

H

-

G

-

F

-

M

-

G

-

H

Y

P

M

G

N

W

W

Y

T

W

Y

M

V

F

F

I

Y

I

L

E

F

G

G

A

L

L

V

A

G

L

I

V

V

A

W

G

F

V

I

A

L

T

W

F

M

C

W

Y

I

L

V

D

S

D

D

R

T

P

P

S

E

E

T

A

H

R

K

F

T

L

I

S

S

Q

H

V

Y

E

E

R

K

D

E

L

Y

L

I

Q

V

K
x
S

T
x
S

L
|
L

A
x
K

A
x
N

E

Q

E

L

S

S

K

Q

S

K

T

V

S

V

K

P

I

W

S

V

D

S

A

I

A

L

R

K

N

S

W

L

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P

V

L

W

W

H

A

L

I

A

V

I

V

I

A

Y

H

M

F

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Y

Q

N

I

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F

Y

Y

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T

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L

V

L

F

T

Y

L

L

L

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P

S

T

Q

Y

V

M

A

K

A

E

L

I

M

L

G

R

T

F

N

N

V

V

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Q

F

-

T

E

A

N

S

G

L

F

V

L

T

S

A

A

I

L

P
|
P

W

Y

V

F

A

G

A

C

L

W

F

L

G

C

T

M

Y

I

Y

V

I

C

P

G

R

Q

Y

A

S

A

D

D

R

Y

T

L

G

R

E

V

R

K

R

W

N

N

I

F

A

S

A

T

L

I

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S

L

V

L

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V

R

A

I

G

F

L

S

G

L

I

I

G
|
G

I

M

S

-

A

-

F

-

A

V

S

G

P

P

V

A

V

V

A

F

I

L

V

V

A

A

L

A

C

G

F

F

A

I

A

G

A

C

G

D

F

Y

I

-

A

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Q

A

P

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V

A

F

F

W

L

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T

M

I

P

S

T

T

G

T

L

L

-

G

-

F

-

A

L

S

S

S

G

G

T

F

A

S

L

I

A

N

-

H

-

L

-

D

-

I

-

A

-

P

-

S

-

Y

A

A

G

G

I

I

-

L

G

G

F

I

T

T

N

N

M

T

F

F

G

A

A

T

F

I

G

P

G

G

F

M

L

I

A

G

P

P

N

I

I

I

K

A

A

K

Q

S

-

L

-

T

A

P

D

D

L

N

F

T

F

I

G

K

N

E

H

W

L

Q

A

T

G

V

L

F

V

C

S

I

L

A

A

A

I

A

I

I

T

N

V

V

I

F

G

G

S

A

I

I

T

F

I

F

M

T

M

L

L

F

P

A

K

K

K

G

E

E

A

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P

Q

R

N

V

W

V

A

L

L

S

S

N

D

R168 (= R110) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ L113) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G114) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E117) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A118) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (= F121) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P122) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ I125) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ T128) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ KTLAA 216:220) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P283) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
G371 (= G320) mutation to V: Remains in the endoplasmic reticulum.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
136 K→E: Markedly decreases H(+)-coupled sialic acid transporter activity.

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
23% identity, 40% coverage: 107:278/427 of query aligns to 165:333/495 of Q8BN82

query
sites
Q8BN82

L

V

V

L

R

R

S

A

L

L

L

E

G

G

A

L

A

G

E

E

A

G

G

V

F

T

F

F

P

P

G

A

M

M

I
x
H

Y

A

L

M

T

W

S

S

Q

S

W

W

F

A

P

P

Q

P

K

L

S

E

R

R

A

S

G

K

I

L

M

L

G

T

L

I

F

S

Y

Y

M

A

G

G

A

A

P

Q

L

L

A

G

L

T

T

V

L

I

G

S

S

L

P

P

L

L

S

S

G

G

A

I

L

I

L

C

E

Y

M

-

H

-

G

-

F

-

M

-

G

-

H

Y

P

M

G

N

W

W

Y

T

W

Y

M

V

F

F

I

Y

I

L

E

F

G

G

A

I

L

V

A

G

L

I

V

V

A

W

G

F

V

I

A

L

T

W

F

M

C

W

Y

I

L

V

D

S

D

D

R

T

P

P

S

E

E

T

A

H

R

K

F

T

L

I

S

S

Q

H

V

Y

E

E

R

K

D

E

L

Y

L

I

Q

V

K

S

T

S

L

L

A

K

A

N

E

Q

E

L

S

S

K

Q

S

K

T

V

S

V

K

P

I

W

S

G

D

S

A

I

A

L

R

K

N

S

W

L

T

P

V

L

W

W

H

A

L

I

A

V

I

V

I

A

Y

H

M

F

I

S

I

Y

Q

N

I

W

S

S

V

F

Y

Y

G

T

L

L

V

L

F

T

Y

L

L

L

P

P

S

T

Q

Y

V

M

A

K

A

E

L

I

M

L

G

R

T

F

N

N

V

V

-

Q

-

E

-

N

G

G

F

F

T

L

A

S

S

A

L

L

H183 (≠ I125) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
23% identity, 68% coverage: 10:298/427 of query aligns to 90:393/570 of Q9C0U9

query
sites
Q9C0U9

N

Q

K

E

T

S

R

K

L

L

R

K

L

W

I

K

P

L

F

Y

M

I

L

Y

T

L

L

L

Y

L

I

M

L

L

A

G

F

F

L

L

D

D

R

M

A

M

-

L

N

F

I

I

G

G

F

K

A

A

K

T

V

L

S

S

Y

Y

Q

S

M

T

D

I

T

L

G

G

L

L

G

F

D

D

G

D

A

V

F

H

A

I

L

T

G

S

A

N

G

Q

-

Y

-

N

-

L

-

N

-

T

I

L

F

F

F

Y

V

V

A

G

Y

Y

A

I

F

V

L

G

G

Q

V

F

P

P

A

G

N

H

L

Y

L

I

M

M

R

Q

K

T

F

F

G

P

A

L

R

G

A

K

W

F

I

V

G

G

S

L

T

V

T

T

L

F

V

S

W

W

G

S

V

V

L

I

S

V

A

F

S

L

M

H

G

C

Y

C

A

A

D

Y

T

N

E

Y

W

G

K

G

F

L

L

I

L

A

V

L

R

R

S

F

L

F

L

L

G

G

A

F

A

T

E

E

A

S

G

C

F

L

P

P

G

A

M

M

I

E

Y

A

L

T

T

M

S

G

Q

M

W

F

F

F

P

T

Q

H

K

Q

S

E

R

Q

A

A

G

F

I

L

M

Q

G

P

L

V

F

F

Y

W

M

I

G

S

A

C

P

-

L

-

A

-

L

L

T

S

L

C

G

G

S

I

P

P

L

-

S

-

G

-

A

-

L

-

E

-

M

-

H

A

G

G

F

F

M

I

G

A

H

Y

-

G

-

L

-

E

-

F

-

V

-

T

-

K

-

S

-

I

P

A

G

P

W

W

Y

K

W

L

M

F

F

M

I

I

E

T

G

G

A

G

L

I

A

T

L

F

V

F

A

L

G

S

V

I

A

F

T

L

F

F

C

F

Y

Y

L

Y

D

P

D

D

R

N

P

P

S

S

E

K

A

A

R

R

F

F

L

L

S

T

Q

D

V

E

E

E

R

K

-

L

-

Y

-

T

-

I

D

D

L

R

L

V

Q

R

K

K

T

S

L

T

A

R

A

G

E

G

E

I

S

E

S

N

K

K

-

I

-

F

S

K

T

K

S

H

K

Q

I

F

S

I

D

E

A

A

A

L

R

K

N

D

W

P

T

I

V

T

W

W

H

L

L

F

A

T

I

F

I

A

Y

A

M

F

I

T

I

L

Q

M

I

L

S

S

-

N

-

L

-

A

-

Y

V

Q

Y

Q

G

N

L

L

V

I

F

F

Y

-

L

T

P

S

S

L

Q

N

V

V

A

S

A

D

L

L

M

N

G

S

T

T

N

L

V

V

G

G

F

V

T

A

A

L

S

A

L

G

V

Y

T

N

A

T

I

V

P

S

W

A

V

I

A

I

A

A

L

T

F

F

G

A

T

M

Y

Y

Y

L

I

I

P

P

R

N

Y

Q

S

S

Sites not aligning to the query:

14 modified: Phosphoserine

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
25% identity, 43% coverage: 102:286/427 of query aligns to 135:319/493 of Q03567

query
sites
Q03567

T

S

E

E

W

Y

K

A

F

L

L

A

L

I

V

L

R

R

S

A

L

A

L

I

G

G

A

F

A

L

E

Q

A

G

G

A

F

T

F

F

P

P

G

A

M

M

I

H

Y

T

L

M

T

W

S

S

Q

V

W

W

F

G

P

P

Q

P

K

L

S

E

R

L

A

S

G

V

I

L

M

T

G

G

L

V

F

T

Y

Y

M

A

G

G

A

A

P

Q

L

I

A

G

L

N

T

V

L

I

G

V

S

L

P

P

L

L

S

S

G

G

A

F

L

L

L

C

E

E

M

-

H

Y

G

G

F

F

M

D

G

G

H

-

P

-

G

G

W

W

Y

P

W

S

M

I

F

F

I

Y

I

I

E

I

G

G

A

V

L

F

A

G

L

V

V

L

A

W

G

T

V

A

A

V

T

W

F

W

C

Y

Y

V

L

S

D

S

D

D

R

K

P

P

S

A

E

T

A

H

R

P

F

R

L

I

S

T

Q

P

V

E

E

E

R

K

D

Q

L

Y

L

I

Q

V

K

T

T

A

L

V

A

E

A

A

-

S

-

M

-

G

E

K

E

D

S

T

S

G

K

K

S

V

T

P

S

S

-

T

-

P

-

W

-

I

K

K

I

I

S

L

D

T

A

S

A

P

R

A

N

V

W

W

T

A

V

C

W

W

-

A

-

G

H

H

L

F

A

A

I

G

I

D

Y

W

M

-

I

-

Q

-

I

-

S

G

V

A

Y

Y

G

T

L

M

V

L

F

V

Y

S

L

L

P

P

S

S

Q

F

V

L

A

K

A

D

L

V

M

L

G

G

T

L

N

N

V

L

G

S

F

-

T

S

A

L

S

G

L

A

V

V

T

A

A

S

I

I

P

P

W

Y

V

I

A

A

Sites not aligning to the query:

69 modified: carbohydrate, N-linked (GlcNAc...) asparagine

A2SWM2 Sphingosine-1-phosphate transporter SPNS2; Protein spinster homolog 2; Protein two of hearts from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
23% identity, 39% coverage: 25:189/427 of query aligns to 66:227/504 of A2SWM2

query
sites
A2SWM2

I

V

L

L

A

N

F

Y

L

M

D

D

R

R

A

Y

N

T

I

V

G

A

F

G

A

V

K

L

V

L

S

D

Y

I

Q

Q

M

K

D

Q

T

F

G

K

L

V

G

G

D

D

G

S

A

S

F

A

A

G

L

L

G

L

A

Q

G

T

I

V

F

F

F

I

V

C

A

S

Y

F

A

M

F

V

L

A

G

A

V

P

P

I

A

F

N

G

L

Y

L

L

M

G

R

D

K

R

F

F

G

N

A

R

R

K

A

I

W

I

I

L

G

S

S

C

T

G

T

I

L

F

V

F

W

W

G

S

V

A

L

V

S

T

A

L

S

L

M

S

G

S

Y

F

A

I

D

T

T

K

E

E

W

Y

K

Y

F

W

L

L

V

V

-

L

-

S

R
|
R

S

C

L

L

L

V

G

G

A

I

A

G

E

E

A

S

G

S

F

Y

F

S

P

S

G

I

M

S

I

P

Y

T

L

I

T

I

S

G

Q

D

W

L

F

F

P

T

Q

N

K

N

S

K

R

R

A

T

G

V

I

M

M

L

G

S

L

V

F

F

Y

Y

M

L

G

A

A

I

P

P

L

L

A

G

L

S

T

G

L

L

G

G

S

Y

P

I

L

L

S

-

G

G

A

S

L

I

L

A

E

K

M

D

H

A

G

G

F

-

M

-

G

G

H

H

P

-

G

-

W

W

Y

Y

W

W

M

A

F

L

I

R

I

V

E

S

G

P

A

M

L

L

A

G

L

L

V

T

A

A

G

G

R153 (= R110) mutation to S: In ko157; displays cardia bifida (2 hearts).

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
22% identity, 50% coverage: 6:219/427 of query aligns to 20:226/448 of Q51955

query
sites
Q51955

E

Q

Q

Q

V

P

V

L

N

S

K

R

T

Y

R

Q

L

W

R

R

L

V

I

V

P

L

F

L

M

C

L

F

T

L

L

I

Y

V

I

F

L

L

A
x
D

F

G

L

L

D
|
D

R

T

A

A

N

A

I

M

G

G

F

F

A

I

K

A

V

P

S

A

Y

L

Q

S

M

Q

D

E

T

W

G

G

L

I

G

D

D

R

G

A

A

S

F

L

A

G

L

P

G

V

A

M

G

S

I

A

F

A

F

L

V

I

A

G

Y

M

A

V

F

F

L

G

G

A

V

L

P

G

A

S

N
x
G

L

P

L

L

M

A

R
x
D

K

R

F

F

G
|
G

A

R

R

K

A

G

W

V

I

L

G

V

S

G

T

A

T

V

L

L

V

V

W

F

G

G

V

G

L

F

S

S

A

L

S

A

M

S

G

A

Y

Y

A

A

D

T

T

N

E

V

W

D

K

Q

F

L

L

L

L

V

V

L

R
|
R

S

F

L

L

L

T

G

G

A

L

A

G

E

L

A

G

G

A

F

G

F

M

P

P

G

N

M

A

I

T

Y

T

L

L

T

L

S

S

Q
x
E

W

Y

F

T

P

P

Q

E

K

R

S

L

R

K

A

S

G

L

I

L

M

V

G

T

L

S

F

M

Y

F

M

C

G

G

A

F

P

N

L

L

A

G

L

M

T

A

L

G

G

G

S

G

P

F

L

I

S

S

G

A

A

K

L

M

L

I

E

P

M

A

H

Y

G

-

F

-

M

-

G

-

H

-

P

-

G

G

W

W

Y
x
H

W

S

M

L

F

L

I

V

I

I

E

G

G

G

A

V

L

L

A

P

L

L

V

L

A

L

G

A

V

L

A

V

T

L

F

M

C

V

Y

W

L

L

D

-

R

-

P

P

S

E

E

S

A

A

R

R

F

F

L

L

S

V

Q

V

V

R

E

N

R

R

-

G

-

T

D

D

L

K

L

I

Q

R

K

K

T

T

L

L

A

S

D41 (≠ A27) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (= D30) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (≠ N71) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (≠ R75) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (= G78) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R110) mutation to A: Abolishes 4-HBA transport.
E144 (≠ Q130) mutation to A: Strong decrease in 4-HBA transport.
H183 (≠ Y175) mutation to A: Decrease in 4-HBA transport and chemotaxis.

Sites not aligning to the query:

323 D→N: Abolishes 4-HBA transport and chemotaxis.
328 H→A: Decrease in 4-HBA transport and chemotaxis.; H→R: Decrease in 4-HBA transport and loss of chemotaxis.
386 R→A: Strong decrease in 4-HBA transport.
398 R→A: Abolishes 4-HBA transport.
444 H→A: No change in 4-HBA transport and chemotaxis.

Query Sequence

>WP_051249719.1 NCBI__GCF_000425265.1:WP_051249719.1
MSPNIEQVVNKTRLRLIPFMLTLYILAFLDRANIGFAKVSYQMDTGLGDGAFALGAGIFF
VAYAFLGVPANLLMRKFGARAWIGSTTLVWGVLSASMGYADTEWKFLLVRSLLGAAEAGF
FPGMIYLTSQWFPQKSRAGIMGLFYMGAPLALTLGSPLSGALLEMHGFMGHPGWYWMFII
EGALALVAGVATFCYLDDRPSEARFLSQVERDLLQKTLAAEESSKSTSKISDAARNWTVW
HLAIIYMIIQISVYGLVFYLPSQVAALMGTNVGFTASLVTAIPWVAALFGTYYIPRYSDR
TGERRNIAALTLLVAGLGIGISAFASPVVAIVALCFAAAGFIAVQPVFWTMPTGLLSGTA
LAAGIGFTNMFGAFGGFLAPNIKAQADLFFGNHLAGLVSLAIITVIGSITIMMLPKKEAP
RVVLSNA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory