SitesBLAST
Comparing WP_051301816.1 NCBI__GCF_000428045.1:WP_051301816.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4pzeA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with acetoacetyl-coa (see paper)
40% identity, 54% coverage: 17:297/525 of query aligns to 4:283/283 of 4pzeA
4pzdA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with NAD+ (see paper)
40% identity, 54% coverage: 17:297/525 of query aligns to 4:283/283 of 4pzdA
- active site: S118 (= S131), H139 (= H152), E151 (= E164), N189 (= N202)
- binding nicotinamide-adenine-dinucleotide: G11 (= G24), T12 (≠ V25), M13 (= M26), D32 (= D45), A88 (= A101), A89 (≠ I102), T90 (≠ V103), E91 (= E104), I99 (≠ L112), N116 (= N129), S118 (= S131), N142 (= N155)
6aa8E Crystal structure of (s)-3-hydroxybutyryl-coenzymea dehydrogenase from clostridium acetobutylicum complexed with NAD+ (see paper)
40% identity, 53% coverage: 20:297/525 of query aligns to 5:281/281 of 6aa8E
- active site: S116 (= S131), H137 (= H152), E149 (= E164), N187 (= N202)
- binding nicotinamide-adenine-dinucleotide: I6 (≠ V21), G9 (= G24), T10 (≠ V25), M11 (= M26), R29 (≠ Y44), D30 (= D45), I31 (≠ A46), A86 (= A101), A87 (≠ I102), E89 (= E104), K94 (= K109), N114 (= N129), S116 (= S131)
4kugA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with NAD from clostridium butyricum
39% identity, 53% coverage: 20:297/525 of query aligns to 6:282/282 of 4kugA
- active site: S117 (= S131), H138 (= H152), E150 (= E164), N188 (= N202)
- binding nicotinamide-adenine-dinucleotide: G10 (= G24), T11 (≠ V25), M12 (= M26), R30 (≠ Y44), D31 (= D45), A87 (= A101), A88 (≠ I102), E90 (= E104), N115 (= N129), S117 (= S131), H138 (= H152)
4kuhA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with acetoacetyl-coa from clostridium butyricum
39% identity, 53% coverage: 20:295/525 of query aligns to 6:280/280 of 4kuhA
P00348 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; L-3-hydroxyacyl CoA dehydrogenase; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Sus scrofa (Pig) (see paper)
37% identity, 53% coverage: 20:296/525 of query aligns to 32:314/314 of P00348
- GGGLMG 34:39 (≠ GAGVMG 22:27) binding NAD(+)
- D57 (= D45) binding NAD(+)
- E122 (= E104) binding NAD(+)
- K127 (= K109) binding NAD(+)
- S149 (= S131) binding NAD(+)
1f0yA L-3-hydroxyacyl-coa dehydrogenase complexed with acetoacetyl-coa and NAD+ (see paper)
37% identity, 53% coverage: 20:296/525 of query aligns to 9:291/291 of 1f0yA
- active site: S126 (= S131), H147 (= H152), E159 (= E164), N197 (= N202)
- binding acetoacetyl-coenzyme a: S50 (≠ I61), K57 (= K68), S126 (= S131), H147 (= H152), F149 (= F154), N150 (= N155), P151 (= P156), P153 (= P158), V154 (≠ L159), N197 (= N202), P232 (≠ R237), M233 (≠ L238), L238 (= L243)
- binding nicotinamide-adenine-dinucleotide: G13 (= G24), L14 (≠ V25), M15 (= M26), D34 (= D45), Q35 (≠ A46), A96 (= A101), I97 (= I102), E99 (= E104), K104 (= K109), N124 (= N129), S126 (= S131), H147 (= H152), N150 (= N155), V242 (≠ T247), T246 (≠ V251), K282 (= K287)
Q16836 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Homo sapiens (Human) (see 7 papers)
38% identity, 53% coverage: 20:296/525 of query aligns to 32:314/314 of Q16836
- GGGLMG 34:39 (≠ GAGVMG 22:27) binding NAD(+)
- A40 (≠ R28) to T: in HADH deficiency; dbSNP:rs137853101
- D57 (= D45) binding NAD(+); to E: in HADH deficiency; dbSNP:rs137853102; to G: found in a patient with Reye-like syndrome; does not affect 3-hydroxyacyl-CoA dehydrogenase activity; increases KM value for NADH; does not affect dimerization
- S73 (≠ I61) binding CoA
- K80 (= K68) binding CoA
- L86 (vs. gap) to P: in dbSNP:rs4956145
- E122 (= E104) binding NAD(+)
- K127 (= K109) binding NAD(+); modified: N6-(2-hydroxyisobutyryl)lysine
- S149 (= S131) binding CoA; binding NAD(+)
- Q152 (≠ S134) to H: in dbSNP:rs1051519
- N173 (= N155) binding NAD(+)
- Y226 (≠ M208) to H: found in a patient with Reye-like syndrome; loss of 3-hydroxyacyl-CoA dehydrogenase activity. Does not affect dimerization; dbSNP:rs146036912
- P258 (= P240) to L: in HHF4; loss of 3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137853103
- K305 (= K287) binding NAD(+)
1f17A L-3-hydroxyacyl-coa dehydrogenase complexed with nadh (see paper)
37% identity, 53% coverage: 20:296/525 of query aligns to 9:291/293 of 1f17A
- active site: S126 (= S131), H147 (= H152), E159 (= E164), N197 (= N202)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G13 (= G24), L14 (≠ V25), M15 (= M26), D34 (= D45), Q35 (≠ A46), A96 (= A101), I97 (= I102), E99 (= E104), K104 (= K109), N124 (= N129), S126 (= S131), H147 (= H152)
1f12A L-3-hydroxyacyl-coa dehydrogenase complexed with 3-hydroxybutyryl-coa (see paper)
37% identity, 53% coverage: 20:296/525 of query aligns to 9:291/293 of 1f12A
- active site: S126 (= S131), H147 (= H152), E159 (= E164), N197 (= N202)
- binding 3-hydroxybutanoyl-coenzyme a: K57 (= K68), S126 (= S131), H147 (= H152), F149 (= F154), N150 (= N155), M155 (= M160), N197 (= N202), L200 (≠ G205), P232 (≠ R237), M233 (≠ L238), L238 (= L243)
P9WNP7 3-hydroxybutyryl-CoA dehydrogenase; Beta-hydroxybutyryl-CoA dehydrogenase; BHBD; EC 1.1.1.157 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
37% identity, 53% coverage: 16:295/525 of query aligns to 6:286/286 of P9WNP7
- S122 (= S131) mutation to A: Loss of fatty acyl dehydrogenase activity.
P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
34% identity, 54% coverage: 18:300/525 of query aligns to 364:644/763 of P40939
- E510 (= E164) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769
Sites not aligning to the query:
- 282 V → D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
- 305 I → N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
- 342 L → P: in LCHAD deficiency; dbSNP:rs137852772
P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12) (see 2 papers)
31% identity, 52% coverage: 34:307/525 of query aligns to 332:604/729 of P21177
- H450 (= H152) active site, For 3-hydroxyacyl-CoA dehydrogenase activity; mutation H->A,Q: Almost complete loss of 3-hydroxyacyl-CoA dehydrogenase activity.
Sites not aligning to the query:
- 116 G→F: Absence of both enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities. Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase is only slightly affected.
- 322 G→A: 10-fold increase in KM for NADH.
6tnmA E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
31% identity, 52% coverage: 34:307/525 of query aligns to 332:604/719 of 6tnmA
Sites not aligning to the query:
1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
33% identity, 51% coverage: 34:300/525 of query aligns to 333:597/715 of 1wdlA
- active site: S430 (= S131), H451 (= H152), E463 (= E164), N501 (= N202)
- binding nicotinamide-adenine-dinucleotide: D344 (= D45), I345 (≠ A46), A400 (= A101), V401 (≠ I102), E403 (= E104), N428 (= N129), T429 (= T130), S430 (= S131)
Sites not aligning to the query:
- active site: 69, 89, 93, 117, 120, 139, 140, 147, 148
- binding nicotinamide-adenine-dinucleotide: 322, 324, 325
P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see paper)
33% identity, 51% coverage: 34:300/525 of query aligns to 333:597/715 of P28793
- D344 (= D45) binding NAD(+)
- VVE 401:403 (≠ IVE 102:104) binding NAD(+)
- K408 (= K109) binding NAD(+)
- S430 (= S131) binding NAD(+)
- N454 (= N155) binding NAD(+)
- N501 (= N202) binding substrate
Sites not aligning to the query:
- 297 binding substrate
- 325 binding NAD(+)
- 660 binding substrate
1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3) (see paper)
33% identity, 50% coverage: 34:297/525 of query aligns to 333:594/707 of 1wdmA
- active site: S430 (= S131), H451 (= H152), E463 (= E164), N501 (= N202)
- binding acetyl coenzyme *a: M459 (= M160), N501 (= N202), P534 (≠ R237)
- binding nicotinamide-adenine-dinucleotide: D344 (= D45), V401 (≠ I102), E403 (= E104), N428 (= N129), S430 (= S131), N454 (= N155)
Sites not aligning to the query:
- active site: 69, 89, 93, 117, 120, 139, 140, 147, 148
- binding acetyl coenzyme *a: 142, 297, 652, 658
- binding nicotinamide-adenine-dinucleotide: 321, 322, 324, 325
6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
33% identity, 53% coverage: 34:313/525 of query aligns to 325:601/707 of 6yswA
Sites not aligning to the query:
- active site: 66, 71, 84, 88, 112, 115, 136, 137, 145, 264
- binding coenzyme a: 23, 25, 66, 67, 68, 136, 137, 140, 290, 293
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
30% identity, 56% coverage: 16:307/525 of query aligns to 292:578/692 of 6iunB
- active site: S407 (= S131), H428 (= H152), E440 (= E164), N478 (= N202)
- binding nicotinamide-adenine-dinucleotide: G300 (= G24), T301 (≠ V25), M302 (= M26), E321 (≠ D45), T322 (≠ A46), Y365 (≠ L89), A377 (= A101), V378 (≠ I102), E380 (= E104), V384 (≠ A108), V388 (≠ L112), N405 (= N129), S407 (= S131)
Sites not aligning to the query:
6zibAAA structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and nadh'
30% identity, 54% coverage: 14:297/525 of query aligns to 300:586/723 of 6zibAAA
- active site: S413 (= S131), H434 (= H152), E446 (= E164), N484 (= N202)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G310 (= G24), T311 (≠ V25), M312 (= M26), E331 (≠ D45), S332 (≠ A46), Q336 (≠ A50), A383 (= A101), V384 (≠ I102), F385 (≠ V103), E386 (= E104), N411 (= N129), H434 (= H152)
Sites not aligning to the query:
- active site: 66, 71, 81, 105, 108, 127, 128, 136, 254
- binding acetoacetyl-coenzyme a: 25, 26, 64, 65, 66, 67, 68, 104, 105, 128, 161
Query Sequence
>WP_051301816.1 NCBI__GCF_000428045.1:WP_051301816.1
MSQQQPNCTKAWVPGETLGVVGAGVMGRGIVQVAAQAGIDVVLYDANSEAVSVAIEEIAH
IFDRLQNKKKLTPEQVVQALSRISCASDLDAFSSCAVVIEAIVERLDAKQGLLGSLERVV
ADDCILATNTSSLSVTSIAAGCTRPERVGGFHCFNPVPLMKLVEVIEGVQTEPRVTDKLT
ALAQQVGHTAVRAQDTPGFVVNHAGRGMITESLRLHSEGVADFRAVDDILRDGVGFRLGP
FELMDLTGLDVSQPVMESIYQQYYQEPRYRPTPLALQRLQAGLLGRKSGKGFYTYEQNQK
LLAPRDEPLPVPLPTIWVSPGNEQTFKQLSGLVEKLGGTVDRGAKPAEDSLCLLAPLGQD
ATTAALELGVDPARAIAIDVIPDLDRLRTLMATPATGEAIKRAAYCLFSSDKVPVVMIRE
SAGFVAQRVLATIINIGCDMAQQRIASPADIDRAVTLGLGYPHGPLAWGDILGANKVVKI
LTNMHQASQDPRYRVSPWLSRRARLGLSLLSDEHIFSEGWTGATV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory