SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_051327420.1 NCBI__GCF_000429905.1:WP_051327420.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
36% identity, 54% coverage: 46:229/343 of query aligns to 72:245/257 of 1ssqD

query
sites
1ssqD

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binding cysteine: D88 (= D62), D153 (= D134), H154 (= H135)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
38% identity, 48% coverage: 54:219/343 of query aligns to 80:235/258 of 4h7oA

query
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4h7oA

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binding arginine: Y127 (≠ K108), Q131 (≠ E112)
binding cysteine: D88 (= D62), P89 (= P63), D153 (= D134), H154 (= H135), G180 (≠ A161), R188 (= R172), H189 (= H173)

Sites not aligning to the query:

binding arginine: 51, 53

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
38% identity, 48% coverage: 54:219/343 of query aligns to 84:239/250 of 4hzdA

query
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4hzdA

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binding coenzyme a: H158 (= H135), G203 (≠ A183), A204 (≠ S184), A221 (≠ N201), A222 (≠ S202), T235 (≠ K215), A237 (≠ S217)
binding magnesium ion: S224 (≠ C204), A237 (≠ S217), G238 (≠ I218)

Sites not aligning to the query:

binding coenzyme a: 244

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
37% identity, 48% coverage: 53:218/343 of query aligns to 107:270/270 of 3p47A

query
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3p47A

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binding cysteine: A208 (= A161), H210 (≠ G163), R224 (= R172), H225 (= H173)

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
37% identity, 48% coverage: 53:216/343 of query aligns to 105:266/267 of 3q1xA

query
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3q1xA

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binding serine: H208 (≠ G163), R222 (= R172), H223 (= H173)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
36% identity, 48% coverage: 51:216/343 of query aligns to 82:237/261 of 6wyeA

query
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6wyeA

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binding (2S)-2-hydroxybutanedioic acid: D93 (= D62), P94 (= P63), D158 (= D134), H159 (= H135), G185 (≠ A161), R193 (= R172), H194 (= H173)

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
36% identity, 48% coverage: 51:216/343 of query aligns to 80:235/243 of 7ra4A

query
sites
7ra4A

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P

D

S

C

I

K

T

V

V

binding serine: D91 (= D62), P92 (= P63), D156 (= D134), H157 (= H135), R191 (= R172), H192 (= H173)

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
37% identity, 47% coverage: 51:211/343 of query aligns to 80:230/243 of 4n69A

query
sites
4n69A

V

A

M

V

S

A

D

D

L

L

E

R

A

A

L

A

T

R

R

E

R

R

D

D

P

P

A

A

A

C

K

V

G

S

D

Y

S

S

V

H

F

C

V

L

L

L

D

N

T

-

Y

Y

K

K

S

G

F

F

E

L

A

A

V

C

M

Q

N

A

Y

H

R

R

L

V

A

A

N

H

N

-

L

-

I

-

N

-

S

-

E

-

L

L

I

L

G

W

K

R

Q

Q

L

S

R

R

L

R

K

P

Y

L

A

A

N

L

K

A

I

L

Q

H

E

S

E

R

S

I

K

A

V

N

A

V

T

F

Q

A

I

V

D

D

I

I

N

H

P

P

S

A

A

A

Q

R

I

I

G

G

K

K

G

G

F

I

I

L

I

F

D

D

H

H

G

A

I

T

G

G

T

V

V

V

I

V

G

G

E

E

T

T

C

A

E

V

I

I

G

G

E

N

N

N

C

V

Y

S

F

I

L

L

N

H

G

H

I

V

V

T

L

L

G

G

A
x
G

T

T

G

G

I

-

A

-

D

-

N

K

E

V

S

G

G

G

R

D

R
|
R

H
|
H

P

P

K

K

I

I

G

G

K

D

D

G

V

V

S

L

I

I

A

G

S

A

F

G

A

A

R

T

L

I

L

L

G

G

P

N

I

I

S

K

V

I

G

G

D

E

R

G

V

A

T

K

I

V

N

G

S

A

F

G

C

S

V

V

I

V

T

L

S

I

D

D

I

V

P

P

binding serine: G183 (≠ A161), R191 (= R172), H192 (= H173)

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
37% identity, 46% coverage: 54:211/343 of query aligns to 80:227/258 of 8i04A

query
sites
8i04A

D

D

L

I

E

Q

A

A

L

V

T

R

R

T

R

R

D
|
D

P
|
P

A

A

A

V

K

D

G

K

D

Y

S

S

V

T

F

P

V

L

L

L

D

-

T

Y

Y

L

K

K

S

G

F

F

E

H

A

A

V

L

M

Q

N

A

Y

Y

R

R

L

I

A

G

N

H

N

W

L

L

I

W

N

N

S

-

E

-

L

-

I

-

G

-

K

-

Q

K

L

G

R

R

L

R

K

A

Y

L

A

A

N

I

K

F

I

L

Q

Q

E

N

E

Q

S

V

K

S

V

V

A

S

T

F

Q

Q

I

V

D

D

I

I

N

H

P

P

S

A

A

A

Q

K

I

I

G

G

K

R

G

G

F

I

I

M

I

L

D
|
D

H
|
H

G

A

I

T

G

G

T

I

V

V

I

V

G

G

E

E

T

T

C

A

E

V

I

I

G

E

E

D

N

D

C

V

Y

S

F

I

L

L

N

Q

G

S

I

V

V

T

L

L

G

G

A
x
G

T

T

G

G

I

-

A

-

D

-

N

K

E

T

S

S

G

G

R

D

R
|
R

H
|
H

P

P

K

K

I

I

G

R

K

E

D

G

V

V

S

M

I

I

A

G

S

A

F

G

A

A

R

K

L

I

L

L

G

G

P

N

I

I

S

E

V

V

G

G

D

R

R

G

V

A

T

K

I

I

N

G

S

A

F

G

C

S

V

V

I

V

T

L

S

Q

D

P

I

V

P

P

binding serine: D88 (= D62), P89 (= P63), D153 (= D134), H154 (= H135), G180 (≠ A161), R188 (= R172), H189 (= H173)

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
37% identity, 46% coverage: 54:211/343 of query aligns to 83:230/246 of 8i09A

query
sites
8i09A

D

D

L

I

E

Q

A

A

L

V

T

R

R

T

R

R

D
|
D

P

P

A

A

A

V

K

D

G

K

D

Y

S

S

V

T

F

P

V

L

L

L

D

-

T

Y

Y

L

K

K

S

G

F

F

E

H

A

A

V

L

M

Q

N

A

Y

Y

R

R

L

I

A

G

N

H

N

W

L

L

I

W

N

N

S

-

E

-

L

-

I

-

G

-

K

-

Q

K

L

G

R

R

L

R

K

A

Y

L

A

A

N

I

K

F

I

L

Q

Q

E

N

E

Q

S

V

K

S

V

V

A

S

T

F

Q

Q

I

V

D

D

I

I

N

H

P

P

S

A

A

A

Q

K

I

I

G

G

K

R

G

G

F

I

I

M

I

L

D
|
D

H
|
H

G

A

I

T

G

G

T

I

V

V

I

V

G
|
G

E

E

T

T

C

A

E

V

I

I

G

E

E

D

N

D

C

V

Y

S

F

I

L

L

N

Q

G

S

I

V

V

T

L

L

G

G

A
x
G

T
|
T

G

G

I

-

A

-

D

-

N

K

E

T

S

S

G

G

R

D

R
|
R

H
|
H

P

P

K

K

I

I

G

R

K

E

D

G

V

V

S

M

I

I

A
x
G

S

A

F

G

A

A

R

K

L

I

L

L

G

G

P

N

I

I

S

E

V

V

G

G

D

R

R

G

V

A

T
x
K

I
|
I

N
x
G

S

A

F

G

C

S

V

V

I

V

T
x
L

S

Q

D

P

I

V

P

P

binding cysteine: D91 (= D62), D156 (= D134), H157 (= H135), G164 (= G142), R191 (= R172), H192 (= H173)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (≠ A161), T184 (= T162), G202 (≠ A183), K218 (≠ T199), I219 (= I200), G220 (≠ N201), L226 (≠ T207)

Sites not aligning to the query:

binding butyl 3,4,5-tris(oxidanyl)benzoate: 236

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
37% identity, 46% coverage: 54:211/343 of query aligns to 84:231/244 of 8i06A

query
sites
8i06A

D

D

L

I

E

Q

A

A

L

V

T

R

R

T

R

R

D

D

P
|
P

A

A

A

V

K

D

G

K

D

Y

S

S

V

T

F

P

V

L

L

L

D

-

T

Y

Y

L

K

K

S

G

F

F

E

H

A

A

V

L

M

Q

N

A

Y

Y

R

R

L

I

A

G

N

H

N

W

L

L

I

W

N

N

S

-

E

-

L

-

I

-

G

-

K

-

Q

K

L

G

R

R

L

R

K

A

Y

L

A

A

N

I

K

F

I

L

Q

Q

E

N

E

Q

S

V

K

S

V

V

A

S

T

F

Q

Q

I

V

D

D

I

I

N

H

P

P

S

A

A

A

Q

K

I

I

G

G

K

R

G

G

F

I

I

M

I

L

D

D

H
|
H

G

A

I

T

G

G

T

I

V

V

I

V

G

G

E

E

T

T

C

A

E

V

I

I

G

E

E

D

N

D

C

V

Y

S

F

I

L
|
L

N
x
Q

G

S

I

V

V

T

L

L

G

G

A

G

T

T

G

G

I

-

A

-

D

-

N

K

E

T

S

S

G

G

R

D

R

R

H

H

P

P

K

K

I

I

G

R

K

E

D

G

V

V

S

M

I

I

A
x
G

S
x
A

F

G

A

A

R

K

L

I

L

L

G

G

P

N

I

I

S

E

V

V

G

G

D

R

R

G

V

A

T

K

I

I

N
x
G

S
x
A

F

G

C

S

V

V

I

V

T

L

S

Q

D

P

I

V

P

P

binding coenzyme a: H158 (= H135), L177 (= L154), Q178 (≠ N155), G203 (≠ A183), A204 (≠ S184), G221 (≠ N201), A222 (≠ S202)
binding cysteine: P93 (= P63), H158 (= H135)

Sites not aligning to the query:

binding coenzyme a: 235, 237

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
37% identity, 46% coverage: 54:211/343 of query aligns to 84:231/262 of 1t3dA

query
sites
1t3dA

D

D

L

I

E

Q

A

A

L

V

T

R

R

T

R

R

D
|
D

P
|
P

A

A

A

V

K

D

G

K

D

Y

S

S

V

T

F

P

V

L

L

L

D

-

T

Y

Y

L

K

K

S

G

F

F

E

H

A

A

V

L

M

Q

N

A

Y

Y

R

R

L

I

A

G

N

H

N

W

L

L

I

W

N

N

S

-

E

-

L

-

I

-

G

-

K

-

Q

Q

L

G

R

R

L

R

K

A

Y

L

A

A

N

I

K

F

I

L

Q

Q

E

N

E

Q

S

V

K

S

V

V

A

T

T

F

Q

Q

I

V

D

D

I

I

N

H

P

P

S

A

A

A

Q

K

I

I

G

G

K

R

G

G

F

I

I

M

I

L

D
|
D

H

H

G

A

I

T

G

G

T

I

V

V

I

V

G

G

E

E

T

T

C

A

E

V

I

I

G

E

E

N

N

D

C

V

Y

S

F

I

L

L

N

Q

G

S

I

V

V

T

L

L

G

G

A
x
G

T

T

G

G

I

-

A

-

D

-

N

K

E

S

S

G

G

G

R

D

R
|
R

H
|
H

P

P

K

K

I

I

G

R

K

E

D

G

V

V

S

M

I

I

A

G

S

A

F

G

A

A

R

K

L

I

L

L

G

G

P

N

I

I

S

E

V

V

G

G

D

R

R

G

V

A

T

K

I

I

N

G

S

A

F

G

C

S

V

V

I

V

T

L

S

Q

D

P

I

V

P

P

binding cysteine: D92 (= D62), P93 (= P63), D157 (= D134), G184 (≠ A161), R192 (= R172), H193 (= H173)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
36% identity, 50% coverage: 49:218/343 of query aligns to 101:260/280 of 7bw9A

query
sites
7bw9A

D

E

F

L

V

V

M

K

S

Q

D

D

L

I

E

I

A

A

L

A

T

Y

R

T

R

G

D
|
D

P
|
P

A

A

A

A

K

S

G

S

D

L

S

A

V

M

F

-

V

I

L

I

D

R

T

S

Y

Y

K

P

S

G

F

I

E

H

A

V

V

M

M

M

N

I

Y

Q

R

R

L

V

A

A

N

H

N

I

L

L

-

Y

I

M

N

N

S

G

E

D

L

I

I

-

G

-

K

-

Q

-

L

-

R

-

L

-

K

E

Y

Y

A

S

N

R

K

E

I

L

Q

M

E

E

E

N

S

I

K

H

V

S

A

V

T

T

Q

G

I

I

D

D

I

I

N

H

P

P

S

G

A

T

Q

S

I

I

G

G

K

N

G

H

F

F

I

F

I

I

D
|
D

H
|
H

G

G

I

V

G

G

T

V

V

V

I

I

G

G

E

E

T

T

C

A

E

V

I

I

G

G

E

N

N

W

C

C

Y

R

F

V

L

Y

N

Q

G

S

I

V

V

T

L

L

G

G

A
|
A

T

M

G

S

I

F

A

K

D

G

N

N

E

-

S

-

G

-

R

K

R
|
R

H
|
H

P

P

K

T

I

I

G

G

K

D

D

F

V

V

S

V

I

I

A

G

S

A

F

G

A

A

R

K

L

V

L

L

G

G

P

N

I

I

S

T

V

I

G

G

D

S

R

N

V

V

T

K

I

I

N

G

S

A

F

N

C

C

V

W

I

I

T

T

S

Q

D

N

I

I

P

D

N

Q

D

D

C

Q

K

I

V

V

S

F

I

I

binding cysteine: D114 (= D62), P115 (= P63), D179 (= D134), H180 (= H135), A206 (= A161), R214 (= R172), H215 (= H173)

1sstA Serine acetyltransferase- complex with coa (see paper)
35% identity, 48% coverage: 46:211/343 of query aligns to 72:220/233 of 1sstA

query
sites
1sstA

S

S

T

I

I

I

D

D

F

C

V

A

M

A

S

C

D

D

L

I

E

Q

A

A

L

V

T

R

R

H

R

R

D

D

P

P

A

A

A

V

K

E

G

L

D

W

S

S

V

T

F

P

V

L

L

L

D

-

T

Y

Y

L

K

K

S

G

F

F

E

H

A

A

V

I

M

Q

N

S

Y

Y

R

R

L

I

A

T

N

H

N

Y

L

L

I

W

N

N

S

-

E

-

L

-

I

-

G

-

K

-

Q

Q

L

N

R

R

L

K

K

S

Y

L

A

A

N

L

K

Y

I

L

Q

Q

E

N

E

Q

S

I

K

S

V

V

A

A

T

F

Q

D

I

V

D

D

I

I

N

H

P

P

S

A

A

A

Q

K

I

I

G

G

K

H

G

G

F

I

I

M

I

F

D

D

H
|
H

G

A

I

T

G

G

T

I

V

V

I

V

G

G

E

E

T

T

C

S

E

V

I

I

G

E

E

N

N

D

C

V

Y

S

F

I

L
|
L

N

Q

G

G

I

V

V

T

L

L

G

G

A
x
G

T
|
T

G

-

I

-

A

-

D

-

N

-

E

-

S

-

G

-

R

-

R

-

H

H

P

P

K

K

I

V

G

R

K

E

D

G

V

V

S

M

I

I

A

G

S
x
A

F

G

A

A

R

K

L

I

L
|
L

G

G

P

N

I

I

S

E

V

V

G

G

D

K

R

Y

V

A

T
x
K

I

I

N
x
G

S
x
A

F

N

C

S

V
|
V

I

V

T
x
L

S

N

D

P

I

V

P

P

binding coenzyme a: H154 (= H135), L173 (= L154), G180 (≠ A161), T181 (= T162), A193 (≠ S184), L198 (= L189), K208 (≠ T199), G210 (≠ N201), A211 (≠ S202), V214 (= V205), L216 (≠ T207)

Sites not aligning to the query:

binding coenzyme a: 224, 226, 228, 229, 233

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
35% identity, 47% coverage: 51:211/343 of query aligns to 76:220/233 of 4n6bA

query
sites
4n6bA

V

A

M

V

S

A

D

D

L

L

E

R

A

A

L

A

T

R

R

E

R

R

D

D

P

V

A

S

A

Y

K

S

G

-

D

-

S

-

V

-

F

H

V

C

L

L

D

L

T

N

Y

Y

K

K

S

G

F

F

E

L

A

A

V

C

M

Q

N

A

Y

H

R

R

L

V

A

A

N

H

N

-

L

-

I

-

N

-

S

-

E

-

L

L

I

L

G

W

K

R

Q

Q

L

S

R

R

L

R

K

P

Y

L

A

A

N

L

K

A

I

L

Q

H

E

S

E

R

S

I

K

A

V

N

A

V

T

F

Q

A

I

V

D

D

I

I

N

H

P

P

S

A

A

A

Q

R

I

I

G

G

K

K

G

G

F

I

I

L

I

F

D

D

H
|
H

G

A

I

T

G

G

T

V

V

V

I

V

G

G

E

E

T

T

C

A

E

V

I

I

G

G

E

N

N

N

C

V

Y

S

F

I

L
|
L

N

H

G

H

I

V

V

T

L

L

G

G

A
x
G

T

T

G

G

I

-

A

-

D

-

N

-

E

-

S

-

G

G

R

D

R

R

H

H

P

P

K

K

I

I

G

G

K

D

D

G

V

V

S

L

I

I

A

G

S

A

F

G

A

A

R

T

L

I

L

L

G

G

P

N

I

I

S

K

V

I

G

G

D

E

R

G

V

A

T
x
K

I

V

N
x
G

S
x
A

F

G

C

S

V

V

I

V

T
x
L

S

I

D

D

I

V

P

P

binding coenzyme a: H150 (= H135), L169 (= L154), G176 (≠ A161), K208 (≠ T199), G210 (≠ N201), A211 (≠ S202), L216 (≠ T207)

Sites not aligning to the query:

binding coenzyme a: 226

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
35% identity, 51% coverage: 38:211/343 of query aligns to 76:234/272 of 3gvdI

query
sites
3gvdI

N

S

D

D

T

A

S

H

I

M

I

I

L

V

S

S

S

A

T

A

I

R

D

D

F

I

V

L

M

-

S

-

D

-

L

-

E

-

A

A

L

V

T

R

R

L

R

R

D
|
D

P
|
P

A

A

A

V

K

D

G

K

D

Y

S

S

V

T

F

P

V

L

L

L

D

-

T

Y

Y

L

K

K

S

G

F

F

E

H

A

A

V

L

M

Q

N

A

Y

Y

R

R

L

I

A

G

N

H

N

W

L

L

I

W

N

-

S

-

E

-

L

-

I

-

G

-

K

A

Q

Q

L

D

R

R

L

K

K

A

Y

L

A

A

N

I

K

Y

I

L

Q

Q

E

N

E

Q

S

V

K

S

V

V

A

A

T

F

Q

G

I

V

D

D

I

I

N

H

P

P

S

A

A

A

Q

T

I

I

G

G

K

C

G

G

F

I

I

M

I

L

D
|
D

H

H

G

A

I

T

G

G

T

I

V

V

I

I

G

G

E

E

T

T

C

A

E

V

I

V

G

E

E

N

N

D

C

V

Y

S

F

I

L

L

N

Q

G

S

I

V

V

T

L

L

G
|
G

A
x
G

T

T

G

G

I

-

A

-

D

-

N

K

E

T

S

S

G

G

R

D

R
|
R

H

H

P

P

K

K

I

I

G

R

K

E

D

G

V

V

S

M

I

I

A

G

S

A

F

G

A

A

R

K

L

I

L

L

G

G

P

N

I

I

S

E

V

V

G

G

D

R

R

G

V

A

T

K

I

I

N

G

S

A

F

G

C

S

V

V

I

V

T

L

S

Q

D

S

I

V

P

P

binding cysteine: D95 (= D62), P96 (= P63), D160 (= D134), G186 (= G160), G187 (≠ A161), R195 (= R172)

4aawA S.Pneumoniae glmu in complex with an antibacterial inhibitor (see paper)
29% identity, 31% coverage: 107:214/343 of query aligns to 307:430/455 of 4aawA

query
sites
4aawA

N

N

K

S

I

M

Q

I

E

E

E

E

S

S

K

S

V

V

A

A

T

D

Q

G

I

V

D

T

I

V

N

G

P

P

S

Y

A

A

Q

H

I

I

-

R

-

P

-

N

-

S

-

S

-

L

G

G

K

A

G

Q

F

V

I

H

I

I

D

G

H

N

G

F

I

V

-

E

-

V

-

K

G

G

T

S

V

S

I

I

G

G

E

E

T

N

C

T

E

K

I

A

G

G

E

H

-

L

-

T

-

Y

-

I

-

G

N

N

C

C

Y

E

F

V

L

G

N

S

G

N

I

V

V

N

L

F

G

G

A

A

T

G

G

T

I

I

A

T

D
x
V

N

N

E
x
Y

S
x
D

G

G

R

K

R

N

H

K

P

Y

K

K

-

T

-

V

I

I

G

G

K

D

D

N

V

V

S

F

I

V

A

G

S

S

F

N

A

S

R

T

L

I

L
x
I

G

A

P

P

I

V

S

E

V

L

G

G

D

D

R

N

V

S

T

L

I

V

N

G

S

A

F

G

C

S

V

T

I

I

T

T

S

K

D

D

I

V

P

P

N

A

D

D

C

A

binding 4-{[1-(2-{[({5-[(3-carboxypropanoyl)amino]-2,4-dimethoxyphenyl}sulfonyl)amino]methyl}phenyl)piperidin-4-yl]methoxy}-4-oxobutanoic acid: V380 (≠ D166), Y382 (≠ E168), D383 (≠ S169), I405 (≠ L189)

Sites not aligning to the query:

active site: 14

4ac3A S.Pneumoniae glmu in complex with an antibacterial inhibitor (see paper)
29% identity, 31% coverage: 107:214/343 of query aligns to 308:431/456 of 4ac3A

query
sites
4ac3A

N

N

K

S

I

M

Q

I

E

E

E

E

S

S

K

S

V

V

A

A

T

D

Q

G

I

V

D

T

I

V

N

G

P

P

S

Y

A

A

Q

H

I

I

-

R

-

P

-

N

-

S

-

S

-

L

G

G

K

A

G

Q

F

V

I

H

I

I

D

G

H

N

G

F

I

V

-

E

-

V

-

K

G

G

T

S

V

S

I

I

G

G

E

E

T

N

C

T

E

K

I

A

G

G

E

H

-

L

-

T

-

Y

-

I

-

G

N

N

C

C

Y

E

F

V

L

G

N

S

G

N

I

V

V

N

L

F

G

G

A

A

T

G

G

T

I

I

A

T

D
x
V

N

N

E
x
Y

S
x
D

G

G

R

K

R

N

H

K

P

Y

K

K

-

T

-

V

I

I

G

G

K

D

D

N

V

V

S

F

I

V

A

G

S

S

F

N

A

S

R

T

L

I

L
x
I

G

A

P

P

I

V

S

E

V

L

G

G

D

D

R

N

V

S

T

L

I

V

N

G

S

A

F

G

C

S

V

T

I

I

T

T

S

K

D

D

I

V

P

P

N

A

D

D

C

A

binding N-{2,4-DIMETHOXY-5-[(2-PIPERIDIN-1-YLBENZYL)sulfamoyl]phenyl}acetamide: V381 (≠ D166), Y383 (≠ E168), D384 (≠ S169), I406 (≠ L189)

Sites not aligning to the query:

active site: 14

7kr9A Bifunctional enzyme glmu bound to zn(ii) (see paper)
29% identity, 31% coverage: 107:214/343 of query aligns to 305:428/453 of 7kr9A

query
sites
7kr9A

N

N

K

S

I

M

Q

I

E
|
E

E

E

S

S

K

S

V

V

A

A

T

D

Q

G

I

V

D

T

I

V

N

G

P

P

S

Y

A

A

Q
x
H

I

I

-

R

-

P

-

N

-

S

-

S

-

L

G

G

K

A

G

Q

F

V

I

H

I

I

D

G

H

N

G

F

I

V

-

E

-

V

-

K

G

G

T

S

V

S

I

I

G

G

E

E

T

N

C

T

E

K

I

A

G

G

E

H

-

L

-

T

-
x
Y

-

I

-

G

N

N

C

C

Y

E

F

V

L

G

N

S

G

N

I

V

V

N

L

F

G

G

A

A

T

G

G

T

I

I

A

T

D
x
V

N
|
N

E
x
Y

S

D

G

G

R

K

R

N

H

K

P

Y

K

K

-

T

-

V

I

I

G

G

K

D

D

N

V

V

S

F

I

V

A

G

S

S

F

N

A

S

R

T

L

I

L

I

G

A

P

P

I

V

S

E

V

L

G

G

D

D

R

N

V

S

T

L

I

V

N

G

S

A

F

G

C

S

V

T

I

I

T

T

S

K

D

D

I

V

P

P

N

A

D

D

C

A

binding acetyl coenzyme *a: Y359 (vs. gap), V378 (≠ D166), N379 (= N167), Y380 (≠ E168)
binding zinc ion: E309 (= E111), H324 (≠ Q126)

1hm9A Crystal structure of s.Pneumoniae n-acetylglucosamine-1-phosphate uridyltransferase, glmu, bound to acetyl coenzyme a and udp-n- acetylglucosamine (see paper)
29% identity, 31% coverage: 107:214/343 of query aligns to 310:433/458 of 1hm9A

query
sites
1hm9A

N

N

K

S

I

M

Q

I

E

E

E

E

S

S

K

S

V

V

A

A

T

D

Q

G

I

V

D

T

I

V

N

G

P

P

S

Y

A

A

Q

H

I

I

-

R

-

P

-

N

-

S

-

S

-

L

G

G

K

A

G

Q

F

V

I

H

I

I

D

G

H

N

G

F

I

V

-

E

-

V

-

K

G

G

T

S

V

S

I

I

G

G

E

E

T

N

C

T

E

K

I

A

G

G

E

H

-

L

-

T

-
x
Y

-

I

-

G

N

N

C

C

Y

E

F

V

L

G

N

S

G

N

I

V

V

N

L

F

G

G

A

A

T

G

G

T

I

I

A

T

D
x
V

N
|
N

E
x
Y

S

D

G

G

R

K

R

N

H

K

P

Y

K

K

-

T

-

V

I

I

G

G

K
x
D

D

N

V

V

S

F

I

V

A

G

S

S

F

N

A

S

R

T

L

I

L

I

G

A

P

P

I

V

S

E

V

L

G

G

D
|
D

R

N

V

S

T

L

I

V

N

G

S

A

F

G

C

S

V

T

I

I

T

T

S

K

D

D

I

V

P

P

N

A

D

D

C

A

binding acetyl coenzyme *a: Y364 (vs. gap), V383 (≠ D166), N384 (= N167), Y385 (≠ E168)
binding calcium ion: D397 (≠ K178), D415 (= D196)

Sites not aligning to the query:

active site: 14
binding calcium ion: 101, 226
binding uridine-diphosphate-n-acetylglucosamine: 7, 8, 9, 10, 71, 74, 76, 77, 137, 138, 153, 168, 169, 196, 198, 226

Query Sequence

>WP_051327420.1 NCBI__GCF_000429905.1:WP_051327420.1
MEQEVDFDLVGKVYQRIGDLLDQHLPKTYRPTIGLTDNDTSIILSSTIDFVMSDLEALTR
RDPAAKGDSVFVLDTYKSFEAVMNYRLANNLINSELIGKQLRLKYANKIQEESKVATQID
INPSAQIGKGFIIDHGIGTVIGETCEIGENCYFLNGIVLGATGIADNESGRRHPKIGKDV
SIASFARLLGPISVGDRVTINSFCVITSDIPNDCKVSIVNQLQVSSHKSFEIYGIVPATN
GNFCIYGSGFPDDVDVSILNIHCDDTGCFIIEKYPLVNIEITDKKNGVIKCQLSLSSENM
DKKEIAKDISMNKNRYHLALSHAEEYAYITPPLALIRTIETLN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory