SitesBLAST
Comparing WP_051402369.1 NCBI__GCF_000496075.1:WP_051402369.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
A4F2N8 L-threo-3-hydroxyaspartate ammonia-lyase; L-threo-3-hydroxyaspartate dehydratase; L-THA DH; EC 4.3.1.16 from Pseudomonas sp. (see paper)
45% identity, 95% coverage: 18:340/340 of query aligns to 2:319/319 of A4F2N8
- K53 (= K69) mutation to A: Loss of enzymatic activity.
Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
41% identity, 89% coverage: 25:328/340 of query aligns to 24:326/339 of Q7XSN8
- E219 (= E224) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
- D225 (= D230) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.
1wtcA Crystal structure of s.Pombe serine racemase complex with amppcp (see paper)
39% identity, 94% coverage: 19:338/340 of query aligns to 2:316/318 of 1wtcA
- active site: K52 (= K69), S77 (= S94), E203 (= E224), G207 (≠ F228), D209 (= D230), G231 (≠ A253), I302 (≠ L324), S303 (= S325)
- binding phosphomethylphosphonic acid adenylate ester: N20 (≠ V37), K47 (≠ R64), M48 (≠ T65), A109 (= A126), A110 (≠ R127), Y114 (≠ S131)
- binding magnesium ion: E203 (= E224), G207 (≠ F228), D209 (= D230)
- binding pyridoxal-5'-phosphate: F51 (= F68), K52 (= K69), N79 (= N96), G178 (≠ S199), G179 (= G200), G180 (= G201), G181 (= G202), G231 (≠ A253), E276 (= E298), T278 (≠ G300), S303 (= S325)
1v71A Crystal structure of s.Pombe serine racemase
39% identity, 94% coverage: 19:338/340 of query aligns to 2:316/318 of 1v71A
- active site: K52 (= K69), S77 (= S94), E203 (= E224), G207 (≠ F228), D209 (= D230), G231 (≠ A253), I302 (≠ L324), S303 (= S325)
- binding magnesium ion: E203 (= E224), G207 (≠ F228), D209 (= D230)
- binding pyridoxal-5'-phosphate: F51 (= F68), K52 (= K69), N79 (= N96), G178 (≠ S199), G179 (= G200), G180 (= G201), G181 (= G202), G231 (≠ A253), E276 (= E298), T278 (≠ G300), S303 (= S325), G304 (= G326)
O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
39% identity, 94% coverage: 19:338/340 of query aligns to 7:321/323 of O59791
- K57 (= K69) active site, Proton acceptor; modified: Lysino-D-alanine (Lys); alternate; modified: N6-(pyridoxal phosphate)lysine; alternate
- S82 (= S94) active site, Proton acceptor; mutation to A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.
- N84 (= N96) binding pyridoxal 5'-phosphate
- G183 (≠ S199) binding pyridoxal 5'-phosphate
- G184 (= G200) binding pyridoxal 5'-phosphate
- G185 (= G201) binding pyridoxal 5'-phosphate
- G186 (= G202) binding pyridoxal 5'-phosphate
- L187 (= L203) binding pyridoxal 5'-phosphate
- E208 (= E224) binding Mg(2+)
- G212 (≠ F228) binding Mg(2+)
- D214 (= D230) binding Mg(2+)
- S308 (= S325) binding pyridoxal 5'-phosphate
2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
39% identity, 94% coverage: 19:338/340 of query aligns to 3:317/319 of 2zr8A
- active site: K53 (= K69), S78 (= S94), E204 (= E224), G208 (≠ F228), D210 (= D230), G232 (≠ A253), I303 (≠ L324), S304 (= S325)
- binding magnesium ion: E204 (= E224), G208 (≠ F228), D210 (= D230)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F68), K53 (= K69), S77 (= S93), S78 (= S94), N80 (= N96), H81 (= H97), P147 (= P163), G179 (≠ S199), G180 (= G200), G181 (= G201), G182 (= G202), G232 (≠ A253), E277 (= E298), T279 (≠ G300), S304 (= S325)
- binding serine: S78 (= S94), R129 (= R145), D231 (= D252), G232 (≠ A253), A233 (≠ L254), Q234 (≠ L255), T235 (≠ A256)
2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
39% identity, 94% coverage: 19:338/340 of query aligns to 3:317/319 of 2zpuA
- active site: K53 (= K69), S78 (= S94), E204 (= E224), G208 (≠ F228), D210 (= D230), G232 (≠ A253), I303 (≠ L324), S304 (= S325)
- binding magnesium ion: E204 (= E224), G208 (≠ F228), D210 (= D230)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F68), K53 (= K69), S77 (= S93), S78 (= S94), N80 (= N96), H81 (= H97), P147 (= P163), G179 (≠ S199), G180 (= G200), G181 (= G201), G182 (= G202), G232 (≠ A253), E277 (= E298), T279 (≠ G300), S304 (= S325)
5cvcA Structure of maize serine racemase (see paper)
40% identity, 90% coverage: 22:328/340 of query aligns to 5:310/329 of 5cvcA
- active site: K52 (= K69), S77 (= S94), E203 (= E224), A207 (≠ F228), D209 (= D230), G231 (≠ A253), V306 (≠ L324), S307 (= S325)
- binding magnesium ion: E203 (= E224), A207 (≠ F228), D209 (= D230)
- binding pyridoxal-5'-phosphate: F51 (= F68), K52 (= K69), N79 (= N96), S178 (= S199), G179 (= G200), G180 (= G201), G181 (= G202), L232 (= L254), E275 (= E298), S307 (= S325), G308 (= G326)
Q9GZT4 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Homo sapiens (Human) (see 4 papers)
38% identity, 91% coverage: 21:328/340 of query aligns to 8:316/340 of Q9GZT4
- E13 (≠ R26) binding Mg(2+)
- S31 (= S44) binding ATP
- S32 (≠ A45) binding ATP
- I33 (= I46) binding ATP
- K51 (≠ R64) binding ATP
- T52 (= T65) binding ATP
- K56 (= K69) modified: N6-(pyridoxal phosphate)lysine
- P69 (≠ A82) binding Ca(2+)
- T81 (≠ A91) binding Ca(2+)
- N86 (= N96) binding pyridoxal 5'-phosphate
- Q89 (= Q99) binding ATP
- Y121 (≠ S131) binding ATP
- D178 (≠ G188) binding Mg(2+)
- G185 (≠ S199) binding pyridoxal 5'-phosphate
- G186 (= G200) binding pyridoxal 5'-phosphate
- G187 (= G201) binding pyridoxal 5'-phosphate
- G188 (= G202) binding pyridoxal 5'-phosphate
- M189 (≠ L203) binding pyridoxal 5'-phosphate
- E210 (= E224) binding Ca(2+); binding Mg(2+); binding Mn(2+)
- A214 (≠ F228) binding Ca(2+); binding Mg(2+); binding Mn(2+)
- D216 (= D230) binding Ca(2+); binding Mg(2+); binding Mn(2+)
- N247 (≠ I262) binding Ca(2+); binding Mg(2+)
- K279 (= K294) binding ATP
- S313 (= S325) binding pyridoxal 5'-phosphate
- N316 (= N328) binding ATP
7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
38% identity, 91% coverage: 21:328/340 of query aligns to 5:313/323 of 7nbfAAA
- active site: K53 (= K69), S81 (= S94), E207 (= E224), A211 (≠ F228), D213 (= D230), G236 (≠ A253), L309 (= L324), S310 (= S325)
- binding calcium ion: E207 (= E224), A211 (≠ F228), D213 (= D230)
- binding magnesium ion: N244 (≠ I262)
- binding pyridoxal-5'-phosphate: F52 (= F68), K53 (= K69), N83 (= N96), G182 (≠ S199), G183 (= G200), G184 (= G201), G185 (= G202), M186 (≠ L203), G236 (≠ A253), V237 (≠ L254), T282 (≠ G300), S310 (= S325), G311 (= G326)
- binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: H21 (≠ V37), L22 (≠ R38), T23 (= T39), P24 (= P40), L26 (≠ V42), T27 (≠ R43), F46 (≠ L62)
Sites not aligning to the query:
7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
38% identity, 91% coverage: 21:328/340 of query aligns to 5:313/323 of 7nbdAAA
- active site: K53 (= K69), S81 (= S94), E207 (= E224), A211 (≠ F228), D213 (= D230), G236 (≠ A253), L309 (= L324), S310 (= S325)
- binding calcium ion: E207 (= E224), A211 (≠ F228), D213 (= D230)
- binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (≠ F290), L278 (≠ V296)
- binding magnesium ion: N244 (≠ I262)
- binding pyridoxal-5'-phosphate: F52 (= F68), K53 (= K69), N83 (= N96), G182 (≠ S199), G183 (= G200), G184 (= G201), G185 (= G202), M186 (≠ L203), G236 (≠ A253), V237 (≠ L254), E280 (= E298), T282 (≠ G300), S310 (= S325), G311 (= G326)
Sites not aligning to the query:
7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
38% identity, 91% coverage: 21:328/340 of query aligns to 5:313/323 of 7nbcCCC
- active site: K53 (= K69), S81 (= S94), E207 (= E224), A211 (≠ F228), D213 (= D230), G236 (≠ A253), L309 (= L324), S310 (= S325)
- binding biphenyl-4-ylacetic acid: T78 (≠ A91), H79 (≠ C92), H84 (= H97), V148 (= V161), H149 (≠ P162), P150 (= P163)
- binding calcium ion: E207 (= E224), A211 (≠ F228), D213 (= D230)
- binding pyridoxal-5'-phosphate: F52 (= F68), K53 (= K69), N83 (= N96), G182 (≠ S199), G183 (= G200), G184 (= G201), G185 (= G202), M186 (≠ L203), G236 (≠ A253), V237 (≠ L254), T282 (≠ G300), S310 (= S325), G311 (= G326)
7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
38% identity, 91% coverage: 21:328/340 of query aligns to 5:313/323 of 7nbcAAA
- active site: K53 (= K69), S81 (= S94), E207 (= E224), A211 (≠ F228), D213 (= D230), G236 (≠ A253), L309 (= L324), S310 (= S325)
- binding calcium ion: E207 (= E224), A211 (≠ F228), D213 (= D230)
- binding magnesium ion: N244 (≠ I262)
- binding pyridoxal-5'-phosphate: F52 (= F68), K53 (= K69), N83 (= N96), G182 (≠ S199), G183 (= G200), G184 (= G201), G185 (= G202), M186 (≠ L203), G236 (≠ A253), V237 (≠ L254), T282 (≠ G300), S310 (= S325), G311 (= G326)
Sites not aligning to the query:
7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
38% identity, 91% coverage: 21:328/340 of query aligns to 5:313/320 of 7nbhAAA