SitesBLAST
Comparing WP_051952709.1 NCBI__GCF_000745855.1:WP_051952709.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
42% identity, 99% coverage: 3:265/266 of query aligns to 2:258/259 of 5zaiC
- active site: A65 (= A66), F70 (≠ V71), S82 (≠ R91), R86 (vs. gap), G110 (= G117), E113 (= E120), P132 (≠ A139), E133 (≠ H140), I138 (≠ V145), P140 (= P147), G141 (= G148), A226 (≠ R233), F236 (≠ R243)
- binding coenzyme a: K24 (≠ A25), L25 (≠ M26), A63 (= A64), G64 (= G65), A65 (= A66), D66 (= D67), I67 (≠ L68), P132 (≠ A139), R166 (≠ T173), F248 (= F255), K251 (= K258)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
37% identity, 98% coverage: 3:263/266 of query aligns to 1:249/250 of 3q0gD
- active site: A64 (= A66), M69 (≠ V71), T75 (≠ L89), F79 (≠ A93), G103 (= G117), E106 (= E120), P125 (≠ A139), E126 (≠ H140), V131 (= V145), P133 (= P147), G134 (= G148), L219 (≠ R233), F229 (≠ R243)
- binding Butyryl Coenzyme A: F225 (≠ L239), F241 (= F255)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
37% identity, 98% coverage: 3:263/266 of query aligns to 2:254/255 of 3q0jC
- active site: A65 (= A66), M70 (≠ V71), T80 (≠ L89), F84 (≠ A93), G108 (= G117), E111 (= E120), P130 (≠ A139), E131 (≠ H140), V136 (= V145), P138 (= P147), G139 (= G148), L224 (≠ R233), F234 (≠ R243)
- binding acetoacetyl-coenzyme a: Q23 (≠ D24), A24 (= A25), L25 (≠ M26), A27 (= A28), A63 (= A64), G64 (= G65), A65 (= A66), D66 (= D67), I67 (≠ L68), K68 (= K69), M70 (≠ V71), F84 (≠ A93), G107 (≠ A116), G108 (= G117), E111 (= E120), P130 (≠ A139), E131 (≠ H140), P138 (= P147), G139 (= G148), M140 (≠ A149)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
37% identity, 98% coverage: 3:263/266 of query aligns to 2:254/255 of 3q0gC
- active site: A65 (= A66), M70 (≠ V71), T80 (≠ L89), F84 (≠ A93), G108 (= G117), E111 (= E120), P130 (≠ A139), E131 (≠ H140), V136 (= V145), P138 (= P147), G139 (= G148), L224 (≠ R233), F234 (≠ R243)
- binding coenzyme a: L25 (≠ M26), A63 (= A64), I67 (≠ L68), K68 (= K69), Y104 (≠ L113), P130 (≠ A139), E131 (≠ H140), L134 (≠ F143)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
37% identity, 98% coverage: 3:263/266 of query aligns to 1:253/256 of 3h81A
- active site: A64 (= A66), M69 (≠ V71), T79 (≠ L89), F83 (≠ A93), G107 (= G117), E110 (= E120), P129 (≠ A139), E130 (≠ H140), V135 (= V145), P137 (= P147), G138 (= G148), L223 (≠ R233), F233 (≠ R243)
- binding calcium ion: F233 (≠ R243), Q238 (≠ I248)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
36% identity, 95% coverage: 11:264/266 of query aligns to 13:256/258 of 1mj3A
- active site: A68 (= A66), M73 (≠ D83), S83 (≠ A93), L85 (≠ M95), G109 (= G117), E112 (= E120), P131 (≠ A139), E132 (≠ H140), T137 (≠ V145), P139 (= P147), G140 (= G148), K225 (≠ R233), F235 (≠ R243)
- binding hexanoyl-coenzyme a: K26 (≠ D24), A27 (= A25), L28 (≠ M26), A30 (= A28), A66 (= A64), G67 (= G65), A68 (= A66), D69 (= D67), I70 (≠ L68), G109 (= G117), P131 (≠ A139), E132 (≠ H140), L135 (≠ F143), G140 (= G148)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
34% identity, 95% coverage: 11:264/266 of query aligns to 11:256/258 of 1ey3A
- active site: A66 (= A66), M71 (≠ V71), S81 (≠ L89), L85 (≠ A93), G109 (= G117), E112 (= E120), P131 (≠ A139), E132 (≠ H140), T137 (≠ V145), P139 (= P147), G140 (= G148), K225 (≠ R233), F235 (≠ R243)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ D24), L26 (≠ M26), A28 (= A28), A64 (= A64), G65 (= G65), A66 (= A66), D67 (= D67), I68 (≠ L68), L85 (≠ A93), W88 (≠ I96), G109 (= G117), P131 (≠ A139), L135 (≠ F143), G140 (= G148)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
34% identity, 95% coverage: 11:264/266 of query aligns to 13:258/260 of 1dubA
- active site: A68 (= A66), M73 (≠ V71), S83 (≠ L89), L87 (≠ A93), G111 (= G117), E114 (= E120), P133 (≠ A139), E134 (≠ H140), T139 (≠ V145), P141 (= P147), G142 (= G148), K227 (≠ R233), F237 (≠ R243)
- binding acetoacetyl-coenzyme a: K26 (≠ D24), A27 (= A25), L28 (≠ M26), A30 (= A28), A66 (= A64), A68 (= A66), D69 (= D67), I70 (≠ L68), Y107 (≠ L113), G110 (≠ A116), G111 (= G117), E114 (= E120), P133 (≠ A139), E134 (≠ H140), L137 (≠ F143), G142 (= G148), F233 (≠ L239), F249 (= F255)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
34% identity, 95% coverage: 11:264/266 of query aligns to 43:288/290 of P14604
- E144 (= E120) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (≠ H140) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
36% identity, 93% coverage: 18:264/266 of query aligns to 20:258/260 of 2hw5C
- active site: A68 (= A66), M73 (≠ V71), S83 (≠ L89), L87 (≠ A93), G111 (= G117), E114 (= E120), P133 (≠ A139), E134 (≠ H140), T139 (≠ V145), P141 (= P147), G142 (= G148), K227 (≠ R233), F237 (≠ R243)
- binding crotonyl coenzyme a: K26 (≠ D24), A27 (= A25), L28 (≠ M26), A30 (= A28), K62 (≠ R60), I70 (≠ L68), F109 (≠ M115)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
35% identity, 95% coverage: 11:264/266 of query aligns to 12:252/254 of 2dubA
- active site: A67 (= A66), M72 (≠ P84), S82 (≠ G94), G105 (= G117), E108 (= E120), P127 (≠ A139), E128 (≠ H140), T133 (≠ V145), P135 (= P147), G136 (= G148), K221 (≠ R233), F231 (≠ R243)
- binding octanoyl-coenzyme a: K25 (≠ D24), A26 (= A25), L27 (≠ M26), A29 (= A28), A65 (= A64), A67 (= A66), D68 (= D67), I69 (≠ L68), K70 (= K69), G105 (= G117), E108 (= E120), P127 (≠ A139), E128 (≠ H140), G136 (= G148), A137 (= A149)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
40% identity, 94% coverage: 17:265/266 of query aligns to 18:260/261 of 5jbxB
- active site: A67 (= A66), R72 (≠ G74), L84 (= L89), R88 (≠ I96), G112 (= G117), E115 (= E120), T134 (≠ A139), E135 (≠ H140), I140 (≠ V145), P142 (= P147), G143 (= G148), A228 (≠ R233), L238 (≠ R243)
- binding coenzyme a: S24 (≠ D24), R25 (≠ A25), R26 (≠ M26), A28 (= A28), A65 (= A64), D68 (= D67), L69 (= L68), K70 (= K69), L110 (≠ M115), G111 (≠ A116), T134 (≠ A139), E135 (≠ H140), L138 (≠ F143), R168 (≠ T173)
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
36% identity, 96% coverage: 10:264/266 of query aligns to 13:264/266 of O53561
- K135 (≠ R135) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 135:142, 25% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ N142) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
38% identity, 96% coverage: 10:265/266 of query aligns to 8:256/257 of 6slbAAA
- active site: Q64 (≠ A66), F69 (≠ G74), L80 (= L89), N84 (≠ A93), A108 (≠ G117), S111 (≠ E120), A130 (= A139), F131 (≠ H140), L136 (≠ V145), P138 (= P147), D139 (≠ G148), A224 (≠ R233), G234 (≠ R243)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (= R60), A62 (= A64), Q64 (≠ A66), D65 (= D67), L66 (= L68), Y76 (≠ D81), A108 (≠ G117), F131 (≠ H140), D139 (≠ G148)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
36% identity, 96% coverage: 10:265/266 of query aligns to 5:244/245 of 6slaAAA
- active site: Q61 (≠ A66), L68 (= L89), N72 (≠ A93), A96 (≠ G117), S99 (≠ E120), A118 (= A139), F119 (≠ H140), L124 (≠ V145), P126 (= P147), N127 (≠ G148), A212 (≠ R233), G222 (≠ R243)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (≠ M26), A59 (= A64), Q61 (≠ A66), D62 (= D67), L63 (= L68), L68 (= L89), Y71 (≠ I92), A94 (≠ M115), G95 (≠ A116), A96 (≠ G117), F119 (≠ H140), I122 (≠ F143), L124 (≠ V145), N127 (≠ G148), F234 (= F255), K237 (= K258)
6l3pA Crystal strcuture of feruloyl-coa hydratase lyase(fchl) complexed with coa
37% identity, 81% coverage: 4:218/266 of query aligns to 7:215/244 of 6l3pA
- active site: M69 (≠ A66), Y74 (≠ V71), R86 (= R91), Q90 (≠ M95), G114 (= G117), S117 (≠ E120), S136 (≠ A139), E137 (≠ H140), I142 (≠ V145), P144 (= P147), G145 (= G148)
- binding coenzyme a: K28 (≠ A25), R29 (≠ M26), A31 (= A28), A67 (= A64), M69 (≠ A66), D70 (= D67), L71 (= L68), G113 (≠ A116)
Sites not aligning to the query:
Q5HH38 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Staphylococcus aureus (strain COL) (see paper)
34% identity, 99% coverage: 1:263/266 of query aligns to 9:266/273 of Q5HH38
- R34 (≠ M26) binding in other chain
- SGGDQ 73:77 (≠ AGADL 64:68) binding in other chain
- S149 (≠ V145) binding in other chain
2uzfA Crystal structure of staphylococcus aureus 1,4-dihydroxy-2-naphthoyl coa synthase (menb) in complex with acetoacetyl coa (see paper)
34% identity, 99% coverage: 1:263/266 of query aligns to 4:253/260 of 2uzfA
- active site: G70 (≠ A66), R80 (≠ L85), L84 (= L89), G108 (= G117), V111 (≠ E120), T130 (≠ A139), G131 (≠ H140), S136 (≠ V145), D138 (≠ P147), A139 (≠ G148), A225 (≠ V238), Y233 (≠ R243)
- binding acetoacetyl-coenzyme a: V28 (≠ A25), R29 (≠ M26), S68 (≠ A64), G69 (= G65), G70 (≠ A66), D71 (= D67), Y104 (≠ L113), G108 (= G117)
Q7CQ56 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
33% identity, 98% coverage: 4:263/266 of query aligns to 22:278/285 of Q7CQ56
3p85A Crystal structure enoyl-coa hydratase from mycobacterium avium (see paper)
37% identity, 89% coverage: 10:247/266 of query aligns to 8:222/224 of 3p85A
- active site: L62 (≠ A66), L67 (≠ V71), P68 (= P79), G92 (= G117), E95 (= E120), T114 (≠ A139), H115 (= H140), L120 (≠ V145), P122 (= P147), T123 (≠ G148), W208 (≠ R233), T219 (≠ H244)
- binding calcium ion: D43 (= D47), D45 (= D49)
Query Sequence
>WP_051952709.1 NCBI__GCF_000745855.1:WP_051952709.1
MHSYTALRYERRAQTAWLTLNRPDAMNALSDALCDELADAIGRIERDRDVRVAVLTGAGR
AFCAGADLKGVFEGGTAAPDDEDPLNAFLDRIAGMIDRLRVLPKPTIAALNGLTMAGGLE
LAMACDLIIAAEGARIGDAHANFGVFPGAGGAAVLPRRIGPTAAKYLLFTGDTLPAAELV
PLGLVNRVVPDAELAAEVDKLATRIASKSPLVLRRMKQAVADGLDQPQATALRLERLVLD
AHRHSHDIREGVAAFVGKRKPEFKGC
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory