SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_051952941.1 NCBI__GCF_000746085.1:WP_051952941.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
62% identity, 88% coverage: 16:259/277 of query aligns to 6:249/250 of 4hzdA

query
sites
4hzdA

I

V

D

D

P

P

I

I

F

W

A

H

R

S

L

I

R

R

N

A

E

E

A

A

Q

E

E

E

V

A

V

T

Q

R

N

N

E

D

P

P

E

V

L

L

A

G

G

A

A

F

I

L

F

Y

S

A

T

T

I

I

L

L

G

N

Q

Q

G

P

T

S

L

L

E

E

A

E

A

A

I

V

I

M

H

H

R

R

I

I

A

A

T

E

R

R

L

L

G

G

H

H

Q

P

A

D

A

V

P

S

A

A

D

D

L

I

I

L

R

R

Q

Q

T

T

Y

F

L

D

D

T

I

M

I

L

A

E

D

A

Q

N

P

P

S

E

I

W

G

S

E

H

A

V

F

L

R

R

A

V

D

D

I

I

I

Q

A

A

V

V

V

Y

E

D

R

R

D

D

P

P

A

A

C

Y

T

S

R

R

I

F

I

M

E

D

P

P

V

V

F

L

Y

Y

F

L

K

K

G

G

F

F

Q

H

A

A

I

I

Q

Q

T

T

H

H

R

R

L

L

A

A

H

H

A

W

L

L

W

Y

S

K

A

Q

G

G

R

R

R

K

D

D

F

F

A

A

L

Y

Y

Y

L

L

Q

Q

S

S

R

R

S

S

S

S

E

S

V

I

F

F

Q

Q

T

T

D

D

I

I

H

H

P

P

A

A

A

A

R

R

I

L

G

G

K

S

G

G

I

L

F

F

L

L

D

D

H
|
H

A

A

T

T

G

G

L

L

V

V

I

V

G

G

A

E

T

T

A

A

V

V

I

V

D

E

D

D

D

N

V

V

S

S

M

I

L

L

Q

H

D

G

V

V

T

T

L

L

G

G

G

G

T

T

G

G

K

K

E

S

K

S

G

G

D

D

R

R

H

H

P

P

K

K

V

I

R

R

S

Q

G

G

V

V

L

L

I

I

G
|
G

A
|
A

G

G

A

A

Q

K

I

I

L

L

G

G

N

N

I

I

E

Q

I

V

G

G

R

Q

C

C

S

S

R

K

I

I

A
|
A

A
|
A

G

G

S
|
S

V

V

V

V

L

L

K

K

P

S

V

V

P

P

D

H

H

N

K

V

T
|
T

V

V

A
|
A

G
|
G

V

V

P

P

A

A

K

R

I

I

V
x
I

G

G

E

E

A

T

G

G

C

C

binding coenzyme a: H158 (= H168), G203 (= G213), A204 (= A214), A221 (= A231), A222 (= A232), T235 (= T245), A237 (= A247), I244 (≠ V254)
binding magnesium ion: S224 (= S234), A237 (= A247), G238 (= G248)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
53% identity, 90% coverage: 19:268/277 of query aligns to 5:254/258 of 4h7oA

query
sites
4h7oA

I

V

F

W

A

Q

R

T

L

I

R

V

N

A

E

E

A

A

Q

R

E

E

V

Q

V

A

Q

E

N

Q

E

E

P

P

E

M

L

L

A

A

G

S

A

F

I

Y

F

H

S

A

T

T

I

I

L

I

G

K

Q

H

G

D

T

S

L

L

E

K

A

A

A

A

I

L

I

S

H

Y

R

I

I

L

A

A

T

N

R

R

L

L

G

N

H
x
T

Q

A

A
x
S

A

M

P

P

A

A

D

M

L

A

I

V

R

R

Q

E

T

V

Y

I

L

E

D

E

I

A

I

F

A

A

D

A

Q

D

P

P

S

S

I

I

G

S

E

E

A

A

F

A

R

A

A

C

D

D

I

I

I

C

A

A

V

T

V

V

E

N

R

R

D
|
D

P
|
P

A

A

C

V

T

S

R

M

I

Y

I

S

E

M

P

P

V

L

F

L

Y

Y

F

L

K

K

G

G

F

Y

Q

H

A

A

I

L

Q

Q

T

G

H

Y

R

R

L

V

A

A

H

N

A

W

L

L

W

W

S

R

A

Q

G

G

R

R

R

K

D

A

F

L

A

A

L

T

Y
|
Y

L

F

Q

Q

S

N

R
x
Q

S

I

S

S

E

V

V

A

F

C

Q

Q

T

V

D

D

I

I

H

H

P

P

A

A

A

A

R

R

I

I

G

G

K

R

G

G

I

I

F

M

L

L

D
|
D

H
|
H

A

A

T

T

G

G

L

I

V

V

I

I

G

G

A

E

T

T

A

A

V

V

I

V

D

E

D

D

D

D

V

V

S

S

M

I

L

L

Q

Q

D

D

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

E

K

C

G

G

D

D

R
|
R

H
|
H

P

P

K

K

V

I

R

R

S

E

G

G

V

V

L

M

I

I

G

G

A

A

G

G

A

A

Q

K

I

I

L

L

G

G

N

N

I

I

E

E

I

V

G

G

R

E

C

G

S

A

R

K

I

I

A

G

A

S

G

G

S

S

V

V

V

V

L

L

K

Q

P

A

V

V

P

P

D

P

H

H

K

T

T

T

V

V

A

A

G

G

V

V

P

P

A

A

K

R

I

I

V

V

G

G

E

R

A

P

G

Q

C

S

A

D

E

K

P

P

S

S

R

L

L

D

M

M

D

D

Q

Q

binding arginine: T51 (≠ H65), S53 (≠ A67), Y127 (= Y141), Q131 (≠ R145)
binding cysteine: D88 (= D102), P89 (= P103), D153 (= D167), H154 (= H168), G180 (= G194), R188 (= R202), H189 (= H203)

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
51% identity, 91% coverage: 16:268/277 of query aligns to 2:254/258 of 8i04A

query
sites
8i04A

I

L

D

E

P

I

I

V

F

W

A

K

R

N

L

I

R

K

N

A

E

E

A

A

Q

R

E

A

V

L

V

A

Q

D

N

C

E

E

P

P

E

M

L

L

A

A

G

S

A

F

I

Y

F

H

S

A

T

T

I

L

L

L

G

K

Q

H

G

E

T

N

L

L

E

G

A

S

A

A

I

L

I

S

H

Y

R

M

I

L

A

A

T

N

R

K

L

L

G

A

H

S

Q

P

A

I

A

M

P

P

A

A

D

I

L

A

I

I

R

R

Q

E

T

V

Y

V

L

E

D

E

I

A

I

Y

A

A

D

A

Q

D

P

P

S

E

I

M

G

I

E

A

A

S

F

A

R

A

A

C

D

D

I

I

I

Q

A

A

V

V

V

R

E

T

R

R

D
|
D

P
|
P

A

A

C

V

T

D

R

K

I

Y

I

S

E

T

P

P

V

L

F

L

Y

Y

F

L

K

K

G

G

F

F

Q

H

A

A

I

L

Q

Q

T

A

H

Y

R

R

L

I

A

G

H

H

A

W

L

L

W

W

S

N

A

K

G

G

R

R

R

R

D

A

F

L

A

A

L

I

Y

F

L

L

Q

Q

S

N

R

Q

S

V

S

S

E

V

V

S

F

F

Q

Q

T

V

D

D

I

I

H

H

P

P

A

A

A

A

R

K

I

I

G

G

K

R

G

G

I

I

F

M

L

L

D
|
D

H
|
H

A

A

T

T

G

G

L

I

V

V

I

V

G

G

A

E

T

T

A

A

V

V

I

I

D

E

D

D

D

D

V

V

S

S

M

I

L

L

Q

Q

D

S

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

T

K

S

G

G

D

D

R
|
R

H
|
H

P

P

K

K

V

I

R

R

S

E

G

G

V

V

L

M

I

I

G

G

A

A

G

G

A

A

Q

K

I

I

L

L

G

G

N

N

I

I

E

E

I

V

G

G

R

R

C

G

S

A

R

K

I

I

A

G

A

A

G

G

S

S

V

V

V

V

L

L

K

Q

P

P

V

V

P

P

D

P

H

H

K

T

T

T

V

A

A

A

G

G

V

V

P

P

A

A

K

R

I

I

V

V

G

G

E

K

A

P

G

G

C

S

A

D

E

K

P

P

S

S

R

M

L

D

M

M

D

D

Q

Q

binding serine: D88 (= D102), P89 (= P103), D153 (= D167), H154 (= H168), G180 (= G194), R188 (= R202), H189 (= H203)

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
51% identity, 90% coverage: 19:268/277 of query aligns to 5:254/257 of 1ssqD

query
sites
1ssqD

I

V

F

W

A

Q

R

H

L

I

R

R

N

Q

E

E

A

A

Q

K

E

E

V

L

V

A

Q

E

N

N

E

E

P

P

E

M

L

L

A

A

G

S

A

F

I

F

F

H

S

S

T

T

I

I

L

L

G

K

Q

H

G

Q

T

N

L

L

E

G

A

G

A

A

I

L

I

S

H

Y

R

L

I

L

A

A

T

N

R

K

L

L

G

A

H

N

Q

P

A

I

A

M

P

P

A

A

D

I

L

S

I

L

R

R

Q

E

T

I

Y

I

L

E

D

E

I

A

I

Y

A

Q

D

S

Q

N

P

P

S

S

I

I

G

I

E

D

A

C

F

A

R

A

A

C

D

D

I

I

I

Q

A

A

V

V

V

R

E

H

R

R

D
|
D

P

P

A

A

C

V

T

E

R

L

I

W

I

S

E

T

P

P

V

L

F

L

Y

Y

F

L

K

K

G

G

F

F

Q

H

A

A

I

I

Q

Q

T

S

H

Y

R

R

L

I

A

T

H

H

A

Y

L

L

W

W

S

N

A

Q

G

N

R

R

R

K

D

S

F

L

A

A

L

L

Y

Y

L

L

Q

Q

S

N

R

Q

S

I

S

S

E

V

V

A

F

F

Q

D

T

V

D

D

I

I

H

H

P

P

A

A

A

A

R

K

I

I

G

G

K

H

G

G

I

I

F

M

L

F

D
|
D

H
|
H

A

A

T

T

G

G

L

I

V

V

I

V

G

G

A

E

T

T

A

S

V

V

I

I

D

E

D

N

D

D

V

V

S

S

M

I

L

L

Q

Q

D

G

V

V

T

T

L

L

G

G

G

G

T

T

G

G

K

K

E

E

K

S

G

G

D

D

R

R

H

H

P

P

K

K

V

V

R

R

S

E

G

G

V

V

L

M

I

I

G

G

A

A

G

G

A

A

Q

K

I

I

L

L

G

G

N

N

I

I

E

E

I

V

G

G

R

K

C

Y

S

A

R

K

I

I

A

G

A

A

G

N

S

S

V

V

V

V

L

L

K

N

P

P

V

V

P

P

D

E

H

Y

K

A

T

T

V

A

A

A

G

G

V

V

P

P

A

A

K

R

I

I

V

V

G

S

E

Q

A

D

G

K

C

A

A

A

E

K

P

P

S

A

R

F

L

D

M

M

D

N

Q

Q

binding cysteine: D88 (= D102), D153 (= D167), H154 (= H168)

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
51% identity, 91% coverage: 16:268/277 of query aligns to 6:258/262 of 1t3dA

query
sites
1t3dA

I

L

D

E

P

I

I

V

F

W

A

N

R

N

L

I

R

K

N

A

E

E

A

A

Q

R

E

T

V

L

V

A

Q

D

N

C

E

E

P

P

E

M

L

L

A

A

G

S

A

F

I

Y

F

H

S

A

T

T

I

L

L

L

G

K

Q

H

G

E

T

N

L

L

E

G

A

S

A

A

I

L

I

S

H

Y

R

M

I

L

A

A

T

N

R

K

L

L

G

S

H

S

Q

P

A

I

A

M

P

P

A

A

D

I

L

A

I

I

R

R

Q

E

T

V

Y

V

L

E

D

E

I

A

I

Y

A

A

D

A

Q

D

P

P

S

E

I

M

G

I

E

A

A

S

F

A

R

A

A

C

D

D

I

I

I

Q

A

A

V

V

V

R

E

T

R

R

D
|
D

P
|
P

A

A

C

V

T

D

R

K

I

Y

I

S

E

T

P

P

V

L

F

L

Y

Y

F

L

K

K

G

G

F

F

Q

H

A

A

I

L

Q

Q

T

A

H

Y

R

R

L

I

A

G

H

H

A

W

L

L

W

W

S

N

A

Q

G

G

R

R

R

R

D

A

F

L

A

A

L

I

Y

F

L

L

Q

Q

S

N

R

Q

S

V

S

S

E

V

V

T

F

F

Q

Q

T

V

D

D

I

I

H

H

P

P

A

A

A

A

R

K

I

I

G

G

K

R

G

G

I

I

F

M

L

L

D
|
D

H

H

A

A

T

T

G

G

L

I

V

V

I

V

G

G

A

E

T

T

A

A

V

V

I

I

D

E

D

N

D

D

V

V

S

S

M

I

L

L

Q

Q

D

S

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

S

K

G

G

G

D

D

R
|
R

H
|
H

P

P

K

K

V

I

R

R

S

E

G

G

V

V

L

M

I

I

G

G

A

A

G

G

A

A

Q

K

I

I

L

L

G

G

N

N

I

I

E

E

I

V

G

G

R

R

C

G

S

A

R

K

I

I

A

G

A

A

G

G

S

S

V

V

V

V

L

L

K

Q

P

P

V

V

P

P

D

P

H

H

K

T

T

T

V

A

A

A

G

G

V

V

P

P

A

A

K

R

I

I

V

V

G

G

E

K

A

P

G

D

C

S

A

D

E

K

P

P

S

S

R

M

L

D

M

M

D

D

Q

Q

binding cysteine: D92 (= D102), P93 (= P103), D157 (= D167), G184 (= G194), R192 (= R202), H193 (= H203)

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
51% identity, 87% coverage: 16:256/277 of query aligns to 5:245/246 of 8i09A

query
sites
8i09A

I

L

D

E

P

I

I

V

F

W

A

K

R

N

L

I

R

K

N

A

E

E

A

A

Q

R

E

A

V

L

V

A

Q

D

N

C

E

E

P

P

E

M

L

L

A

A

G

S

A

F

I

Y

F

H

S

A

T

T

I

L

L

L

G

K

Q

H

G

E

T

N

L

L

E

G

A

S

A

A

I

L

I

S

H

Y

R

M

I

L

A

A

T

N

R

K

L

L

G

A

H

S

Q

P

A

I

A

M

P

P

A

A

D

I

L

A

I

I

R

R

Q

E

T

V

Y

V

L

E

D

E

I

A

I

Y

A

A

D

A

Q

D

P

P

S

E

I

M

G

I

E

A

A

S

F

A

R

A

A

C

D

D

I

I

I

Q

A

A

V

V

V

R

E

T

R

R

D
|
D

P

P

A

A

C

V

T

D

R

K

I

Y

I

S

E

T

P

P

V

L

F

L

Y

Y

F

L

K

K

G

G

F

F

Q

H

A

A

I

L

Q

Q

T

A

H

Y

R

R

L

I

A

G

H

H

A

W

L

L

W

W

S

N

A

K

G

G

R

R

R

R

D

A

F

L

A

A

L

I

Y

F

L

L

Q

Q

S

N

R

Q

S

V

S

S

E

V

V

S

F

F

Q

Q

T

V

D

D

I

I

H

H

P

P

A

A

A

A

R

K

I

I

G

G

K

R

G

G

I

I

F

M

L

L

D
|
D

H
|
H

A

A

T

T

G

G

L

I

V

V

I

V

G
|
G

A

E

T

T

A

A

V

V

I

I

D

E

D

D

D

D

V

V

S

S

M

I

L

L

Q

Q

D

S

V

V

T

T

L

L

G

G

G
|
G

T
|
T

G

G

K

K

E

T

K

S

G

G

D

D

R
|
R

H
|
H

P

P

K

K

V

I

R

R

S

E

G

G

V

V

L

M

I

I

G
|
G

A

A

G

G

A

A

Q

K

I

I

L

L

G

G

N

N

I

I

E

E

I

V

G

G

R

R

C

G

S

A

R
x
K

I
|
I

A
x
G

A

A

G

G

S

S

V

V

V

V

L
|
L

K

Q

P

P

V

V

P

P

D

P

H

H

K

T

T

T

V

A

A
|
A

G

G

V

V

P

P

A

A

K

R

I

I

V

V

G

G

E

K

binding cysteine: D91 (= D102), D156 (= D167), H157 (= H168), G164 (= G175), R191 (= R202), H192 (= H203)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (= G194), T184 (= T195), G202 (= G213), K218 (≠ R229), I219 (= I230), G220 (≠ A231), L226 (= L237), A236 (= A247)

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
51% identity, 86% coverage: 16:254/277 of query aligns to 6:244/244 of 8i06A

query
sites
8i06A

I

L

D

E

P

I

I

V

F

W

A

K

R

N

L

I

R

K

N

A

E

E

A

A

Q

R

E

A

V

L

V

A

Q

D

N

C

E

E

P

P

E

M

L

L

A

A

G

S

A

F

I

Y

F

H

S

A

T

T

I

L

L

L

G

K

Q

H

G

E

T

N

L

L

E

G

A

S

A

A

I

L

I

S

H

Y

R

M

I

L

A

A

T

N

R

K

L

L

G

A

H

S

Q

P

A

I

A

M

P

P

A

A

D

I

L

A

I

I

R

R

Q

E

T

V

Y

V

L

E

D

E

I

A

I

Y

A

A

D

A

Q

D

P

P

S

E

I

M

G

I

E

A

A

S

F

A

R

A

A

C

D

D

I

I

I

Q

A

A

V

V

V

R

E

T

R

R

D

D

P
|
P

A

A

C

V

T

D

R

K

I

Y

I

S

E

T

P

P

V

L

F

L

Y

Y

F

L

K

K

G

G

F

F

Q

H

A

A

I

L

Q

Q

T

A

H

Y

R

R

L

I

A

G

H

H

A

W

L

L

W

W

S

N

A

K

G

G

R

R

R

R

D

A

F

L

A

A

L

I

Y

F

L

L

Q

Q

S

N

R

Q

S

V

S

S

E

V

V

S

F

F

Q

Q

T

V

D

D

I

I

H

H

P

P

A

A

A

A

R

K

I

I

G

G

K

R

G

G

I

I

F

M

L

L

D

D

H
|
H

A

A

T

T

G

G

L

I

V

V

I

V

G

G

A

E

T

T

A

A

V

V

I

I

D

E

D

D

D

D

V

V

S

S

M

I

L
|
L

Q
|
Q

D

S

V

V

T

T

L

L

G

G

G

G

T

T

G

G

K

K

E

T

K

S

G

G

D

D

R

R

H

H

P

P

K

K

V

I

R

R

S

E

G

G

V

V

L

M

I

I

G
|
G

A
|
A

G

G

A

A

Q

K

I

I

L

L

G

G

N

N

I

I

E

E

I

V

G

G

R

R

C

G

S

A

R

K

I

I

A
x
G

A
|
A

G

G

S

S

V

V

V

V

L

L

K

Q

P

P

V

V

P

P

D

P

H

H

K

T

T
|
T

V

A

A
|
A

G

G

V

V

P

P

A

A

K

R

I

I

V

V

binding coenzyme a: H158 (= H168), L177 (= L187), Q178 (= Q188), G203 (= G213), A204 (= A214), G221 (≠ A231), A222 (= A232), T235 (= T245), A237 (= A247)
binding cysteine: P93 (= P103), H158 (= H168)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
50% identity, 91% coverage: 16:268/277 of query aligns to 9:261/272 of 3gvdI

query
sites
3gvdI

I

L

D

E

P

Q

I

V

F

W

A

S

R

N

L

I

R

K

N

S

E

E

A

A

Q

R

E

A

V

L

V

A

Q

E

N

C

E

E

P

P

E

M

L

L

A

A

G

S

A

F

I

F

F

H

S

A

T

T

I

L

L

L

G

K

Q

H

G

E

T

N

L

L

E

G

A

S

A

A

I

L

I

S

H

Y

R

I

I

L

A

A

T

N

R

K

L

L

G

A

H

N

Q

P

A

I

A

M

P

P

A

A

D

I

L

A

I

I

R

R

Q

E

T

V

Y

V

L

E

D

E

I

A

I

Y

-

R

A

S

D

D

Q

A

P

H

S

M

I

I

G

V

E

S

A

A

F

A

R

R

A

-

D

D

I

I

I

L

A

A

V

V

V

R

E

L

R

R

D
|
D

P
|
P

A

A

C

V

T

D

R

K

I

Y

I

S

E

T

P

P

V

L

F

L

Y

Y

F

L

K

K

G

G

F

F

Q

H

A

A

I

L

Q

Q

T

A

H

Y

R

R

L

I

A

G

H

H

A

W

L

L

W

W

S

A

A

Q

G

D

R

R

R

K

D

A

F

L

A

A

L

I

Y

Y

L

L

Q

Q

S

N

R

Q

S

V

S

S

E

V

V

A

F

F

Q

G

T

V

D

D

I

I

H

H

P

P

A

A

A

A

R

T

I

I

G

G

K

C

G

G

I

I

F

M

L

L

D
|
D

H

H

A

A

T

T

G

G

L

I

V

V

I

I

G

G

A

E

T

T

A

A

V

V

I

V

D

E

D

N

D

D

V

V

S

S

M

I

L

L

Q

Q

D

S

V

V

T

T

L

L

G
|
G

G
|
G

T

T

G

G

K

K

E

T

K

S

G

G

D

D

R
|
R

H

H

P

P

K

K

V

I

R

R

S

E

G

G

V

V

L

M

I

I

G

G

A

A

G

G

A

A

Q

K

I

I

L

L

G

G

N

N

I

I

E

E

I

V

G

G

R

R

C

G

S

A

R

K

I

I

A

G

A

A

G

G

S

S

V

V

V

V

L

L

K

Q

P

S

V

V

P

P

D

A

H

H

K

T

T

T

V

A

A

A

G

G

V

V

P

P

A

A

K

R

I

I

V

V

G

G

E

K

A

P

G

E

C

S

A

D

E

K

P

P

S

S

R

L

L

D

M

M

D

D

Q

Q

binding cysteine: D95 (= D102), P96 (= P103), D160 (= D167), G186 (= G193), G187 (= G194), R195 (= R202)

1sstA Serine acetyltransferase- complex with coa (see paper)
50% identity, 85% coverage: 19:254/277 of query aligns to 5:233/233 of 1sstA

query
sites
1sstA

I

V

F

W

A

Q

R

H

L

I

R

R

N

Q

E

E

A

A

Q

K

E

E

V

L

V

A

Q

E

N

N

E

E

P

P

E

M

L

L

A

A

G

S

A

F

I

F

F

H

S

S

T

T

I

I

L

L

G

K

Q

H

G

Q

T

N

L

L

E

G

A

G

A

A

I

L

I

S

H

Y

R

L

I

L

A

A

T

N

R

K

L

L

G

A

H

N

Q

P

A

I

A

M

P

P

A

A

D

I

L

S

I

L

R

R

Q

E

T

I

Y

I

L

E

D

E

I

A

I

Y

A

Q

D

S

Q

N

P

P

S

S

I

I

G

I

E

D

A

C

F

A

R

A

A

C

D

D

I

I

I

Q

A

A

V

V

V

R

E

H

R

R

D

D

P

P

A

A

C

V

T

E

R

L

I

W

I

S

E

T

P

P

V

L

F

L

Y

Y

F

L

K

K

G

G

F

F

Q

H

A

A

I

I

Q

Q

T

S

H

Y

R

R

L

I

A

T

H

H

A

Y

L

L

W

W

S

N

A

Q

G

N

R

R

R

K

D

S

F

L

A

A

L

L

Y

Y

L

L

Q

Q

S

N

R

Q

S

I

S

S

E

V

V

A

F

F

Q

D

T

V

D

D

I

I

H

H

P

P

A

A

A

A

R

K

I

I

G

G

K

H

G

G

I

I

F

M

L

F

D

D

H
|
H

A

A

T

T

G

G

L

I

V

V

I

V

G

G

A

E

T

T

A

S

V

V

I

I

D

E

D

N

D

D

V

V

S

S

M

I

L
|
L

Q

Q

D

G

V

V

T

T

L

L

G

G

G
|
G

T
|
T

G

-

K

-

E

-

K

-

G

-

D

-

R

-

H

H

P

P

K

K

V

V

R

R

S

E

G

G

V

V

L

M

I

I

G

G

A
|
A

G

G

A

A

Q

K

I

I

L
|
L

G

G

N

N

I

I

E

E

I

V

G

G

R

K

C

Y

S

A

R
x
K

I

I

A
x
G

A
|
A

G

N

S

S

V
|
V

V

V

L
|
L

K

N

P

P

V

V

P

P

D

E

H

Y

K

A

T
|
T

V

A

A
|
A

G

G

V
|
V

P
|
P

A

A

K

R

I

I

V
|
V

binding coenzyme a: H154 (= H168), L173 (= L187), G180 (= G194), T181 (= T195), A193 (= A214), L198 (= L219), K208 (≠ R229), G210 (≠ A231), A211 (= A232), V214 (= V235), L216 (= L237), T224 (= T245), A226 (= A247), V228 (= V249), P229 (= P250), V233 (= V254)

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
49% identity, 85% coverage: 19:254/277 of query aligns to 8:243/243 of 4n69A

query
sites
4n69A

I

V

F

W

A

G

R

Q

L

I

R

K

N

A

E

E

A

A

Q

R

E

R

V

D

V

A

Q

E

N

S

E

E

P

P

E

A

L

L

A

A

G

S

A

Y

I

L

F

Y

S

S

T

T

I

I

L

L

G

S

Q

H

G

S

T

S

L

L

E

E

A

R

A

S

I

L

I

S

H

F

R

H

I

L

A

G

T

N

R

K

L

L

G

C

H

S

Q

S

A

T

A

L

P

L

A

S

D

T

L

L

I

L

R

Y

Q

D

T

L

Y

F

L

L

D

N

I

A

I

F

A

S

D

S

Q

D

P

P

S

S

I

L

G

R

E

S

A

A

F

A

R

V

A

A

D

D

I

L

I

R

A

A

V

A

V

R

E

E

R

R

D

D

P

P

A

A

C

C

T

V

R

S

I

Y

I

S

E

H

P

C

V

L

F

L

Y

N

F

Y

K

K

G

G

F

F

Q

L

A

A

I

C

Q

Q

T

A

H

H

R

R

L

V

A

A

H

H

A

L

L

L

W

W

S

R

A

Q

G

S

R

R

R

R

D

P

F

L

A

A

L

L

Y

A

L

L

Q

H

S

S

R

R

S

I

S

A

E

N

V

V

F

F

Q

A

T

V

D

D

I

I

H

H

P

P

A

A

A

A

R

R

I

I

G

G

K

K

G

G

I

I

F

L

L

F

D

D

H

H

A

A

T

T

G

G

L

V

V

V

I

V

G

G

A

E

T

T

A

A

V

V

I

I

D

G

D

N

D

N

V

V

S

S

M

I

L

L

Q

H

D

H

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

K

E

V

K

G

G

G

D

D

R
|
R

H
|
H

P

P

K

K

V

I

R

G

S

D

G

G

V

V

L

L

I

I

G

G

A

A

G

G

A

A

Q

T

I

I

L

L

G

G

N

N

I

I

E

K

I

I

G

G

R

E

C

G

S

A

R

K

I

V

A

G

A

A

G

G

S

S

V

V

V

V

L

L

K

I

P

D

V

V

P

P

D

P

H

R

K

T

T

T

V

A

A

V

G

G

V

N

P

P

A

A

K

R

I

L

V

V

binding serine: G183 (= G194), R191 (= R202), H192 (= H203)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
46% identity, 91% coverage: 19:270/277 of query aligns to 10:260/261 of 6wyeA

query
sites
6wyeA

I

L

F

W

A

H

R

T

L

I

R

R

N

E

E

E

A

T

Q

A

E

A

V

A

V

A

Q

A

N

A

E

E

P

P

E

M

L

L

A

A

G

S

A

F

I

L

F

H

S

Q

T

T

I

V

L

L

G

R

Q

H

G

E

T

S

L

L

E

G

A

S

A

V

I

L

I

A

H

Y

R

H

I

L

A

S

T

S

R

K

L

L

G

G

H

S

Q

P

A

I

A

M

P

D

A

V

D

R

L

A

I

L

R

F

Q

E

T

I

Y

Y

L

Q

D

Q

I

A

I

L

A

G

D

S

Q

D

P

T

S

Q

I

I

G

S

E

K

A

C

F

V

R

E

A

A

D

D

I

L

I

K

A

A

V

I

V

Y

E

E

R

R

D
|
D

P
|
P

A

A

C

C

T

D

R

E

I

Y

I

S

E

L

P

P

V

L

F

L

Y

Y

F

F

K

K

G

G

F

F

Q

H

A

A

I

I

Q

Q

T

A

H

H

R

R

L

I

A

N

H

H

A

R

L

L

W

Y

S

L

A

D

G

G

R

R

R

K

D

T

F

L

A

A

L

Y

Y

F

L

L

Q

Q

S

N

R

R

S

M

S

S

E

E

V

V

F

F

Q

G

T

V

D

D

I

I

H

H

P

P

A

A

A

A

R

R

I

L

G

G

K

Y

G

G

I

L

F

M

L

L

D
|
D

H
|
H

A

A

T

T

G

G

L

F

V

V

I

A

G

G

A

E

T

T

A

A

V

V

I

L

D

G

D

N

D

N

V

I

S

S

M

I

L

L

Q

H

D

G

V

V

T

T

L

L

G

G

G
|
G

T

S

G

G

K

K

E

E

K

G

G

G

D

D

R
|
R

H
|
H

P

P

K

K

V

I

R

G

S

D

G

G

V

V

L

M

I

I

G

G

A

A

G

N

A

A

Q

S

I

I

L

L

G

G

N

N

I

I

E

R

I

I

G

G

R

S

C

N

S

A

R

K

I

I

A

G

A

A

G

G

S

S

V

V

V

V

L

V

K

S

P

D

V

V

P

P

D

P

H

S

K

I

T

T

V

V

A

V

G

G

V

V

P

P

A

A

K

K

I

P

V

V

G

A

E

R

A

S

G

-

C

L

A

K

E

T

P

P

S

S

R

A

L

D

M

M

D

D

Q

Q

I

N

I

I

binding (2S)-2-hydroxybutanedioic acid: D93 (= D102), P94 (= P103), D158 (= D167), H159 (= H168), G185 (= G194), R193 (= R202), H194 (= H203)

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
47% identity, 85% coverage: 19:254/277 of query aligns to 8:243/243 of 7ra4A

query
sites
7ra4A

I

L

F

W

A

H

R

T

L

I

R

R

N

E

E

E

A

T

Q

A

E

A

V

A

V

A

Q

A

N

A

E

E

P

P

E

M

L

L

A

A

G

S

A

F

I

L

F

H

S

Q

T

T

I

V

L

L

G

R

Q

H

G

E

T

S

L

L

E

G

A

S

A

V

I

L

I

A

H

Y

R

H

I

L

A

S

T

S

R

K

L

L

G

G

H

S

Q

P

A

I

A

M

P

D

A

V

D

R

L

A

I

L

R

F

Q

E

T

I

Y

Y

L

Q

D

Q

I

A

I

L

A

G

D

S

Q

D

P

T

S

Q

I

I

G

S

E

K

A

C

F

V

R

E

A

A

D

D

I

L

I

K

A

A

V

I

V

Y

E

E

R

R

D
|
D

P
|
P

A

A

C

C

T

D

R

E

I

Y

I

S

E

L

P

P

V

L

F

L

Y

Y

F

F

K

K

G

G

F

F

Q

H

A

A

I

I

Q

Q

T

A

H

H

R

R

L

I

A

N

H

H

A

R

L

L

W

Y

S

L

A

D

G

G

R

R

R

K

D

T

F

L

A

A

L

Y

Y

F

L

L

Q

Q

S

N

R

R

S

M

S

S

E

E

V

V

F

F

Q

G

T

V

D

D

I

I

H

H

P

P

A

A

A

A

R

R

I

L

G

G

K

Y

G

G

I

L

F

M

L

L

D
|
D

H
|
H

A

A

T

T

G

G

L

F

V

V

I

A

G

G

A

E

T

T

A

A

V

V

I

L

D

G

D

N

D

N

V

I

S

S

M

I

L

L

Q

H

D

G

V

V

T

T

L

L

G

G

G

G

T

S

G

G

K

K

E

E

K

G

G

G

D

D

R
|
R

H
|
H

P

P

K

K

V

I

R

G

S

D

G

G

V

V

L

M

I

I

G

G

A

A

G

N

A

A

Q

S

I

I

L

L

G

G

N

N

I

I

E

R

I

I

G

G

R

S

C

N

S

A

R

K

I

I

A

G

A

A

G

G

S

S

V

V

V

V

L

V

K

S

P

D

V

V

P

P

D

P

H

S

K

I

T

T

V

V

A

V

G

G

V

V

P

P

A

A

K

K

I

P

V

V

binding serine: D91 (= D102), P92 (= P103), D156 (= D167), H157 (= H168), R191 (= R202), H192 (= H203)

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
47% identity, 85% coverage: 19:254/277 of query aligns to 4:233/233 of 4n6bA

query
sites
4n6bA

I

V

F

W

A

G

R

Q

L

I

R

K

N

A

E

E

A

A

Q

R

E

R

V

D

V

A

Q

E

N

S

E

E

P

P

E

A

L

L

A

A

G

S

A

Y

I

L

F

Y

S

S

T

T

I

I

L

L

G

S

Q

H

G

S

T

S

L

L

E

E

A

R

A

S

I

L

I

S

H

F

R

H

I

L

A

G

T

N

R

K

L

L

G

C

H

S

Q

S

A

T

A

L

P

L

A

S

D

T

L

L

I

L

R

Y

Q

D

T

L

Y

F

L

L

D

N

I

A

I

F

A

S

D

S

Q

D

P

P

S

S

I

L

G

R

E

S

A

A

F

A

R

V

A

A

D

D

I

L

I

R

A

A

V

A

V

R

E

E

R

R

D

D

P

V

A

S

C

Y

T

S

R

H

I

C

I

L

E

-

P

-

V

-

F

L

Y

N

F

Y

K

K

G

G

F

F

Q

L

A

A

I

C

Q

Q

T

A

H

H

R

R

L

V

A

A

H

H

A

L

L

L

W

W

S

R

A

Q

G

S

R

R

R

R

D

P

F

L

A

A

L

L

Y

A

L

L

Q

H

S

S

R

R

S

I

S

A

E

N

V

V

F

F

Q

A

T

V

D

D

I

I

H

H

P

P

A

A

A

A

R

R

I

I

G

G

K

K

G

G

I

I

F

L

L

F

D

D

H
|
H

A

A

T

T

G

G

L

V

V

V

I

V

G

G

A

E

T

T

A

A

V

V

I

I

D

G

D

N

D

N

V

V

S

S

M

I

L
|
L

Q

H

D

H

V

V

T

T

L

L

G

G

G
|
G

T

T

G

G

K

-

E

-

K

-

G

G

D

D

R

R

H

H

P

P

K

K

V

I

R

G

S

D

G

G

V

V

L

L

I

I

G

G

A

A

G

G

A

A

Q

T

I

I

L

L

G

G

N

N

I

I

E

K

I

I

G

G

R

E

C

G

S

A

R
x
K

I

V

A
x
G

A
|
A

G

G

S

S

V

V

V

V

L
|
L

K

I

P

D

V

V

P

P

D

P

H

R

K

T

T

T

V

A

A
x
V

G

G

V

N

P

P

A

A

K

R

I

L

V

V

binding coenzyme a: H150 (= H168), L169 (= L187), G176 (= G194), K208 (≠ R229), G210 (≠ A231), A211 (= A232), L216 (= L237), V226 (≠ A247)

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
35% identity, 62% coverage: 74:246/277 of query aligns to 88:268/270 of 3p47A

query
sites
3p47A

R

H

Q

Q

T

C

Y

V

L

M

D

A

I

I

I

L

A

E

D

K

Q

L

P

P

S

S

I

I

G

K

E

R

A

T

F

L

R

K

A

T

D

D

I

L

I

I

A

A

V

A

V

Y

E

A

R

G

D

D

P

P

A

A

C

A

T

P

R

G

I

L

I

S

E

L

P

I

V

I

F

R

Y

C

F

Y

K

P

G

G

F

F

Q

Q

A

A

I

V

Q

I

T

V

H

Y

R

R

L

I

A

A

H

H

A

V

L

L

W

Y

S

E

A

C

G

G

R

E

R

R

D

Y

F

Y

A

C

L

R

Y

E

L

M

Q

M

S

E

R

S

S

V

S

H

E

S

V

Y

F

T

Q

S

T

I

D

D

I

I

H

H

P

P

A

G

A

A

R

S

I

I

G

K

K

G

G

H

I

F

F

F

L

I

D

D

H

H

A

G

T

V

G

G

L

V

V

V

I

I

G

G

A

E

T

T

A

A

V

I

I

I

D

G

D

E

D

W

V

C

S

R

M

I

L

Y

Q

Q

D

S

V

V

T

T

L

L

-

G

-
x
A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

G

G

T

V

G

I

K

K

E

R

K

G

G

T

D

K

R
|
R

H
|
H

P

P

K

T

V

V

R

G

S

D

G

Y

V

V

L

T

I

I

G

G

A

T

G

G

A

A

Q

K

I

V

L

L

G

G

N

N

I

I

E

I

I

V

G

G

R

S

C

H

S

V

R

R

I

I

A

G

A

A

G

N

S

C

V

W

V

I

L

D

K

R

P

D

V

V

P

D

D

S

H

N

K

Q

T

T

V

V

binding cysteine: A208 (vs. gap), H210 (vs. gap), R224 (= R202), H225 (= H203)

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
35% identity, 62% coverage: 74:246/277 of query aligns to 86:266/267 of 3q1xA

query
sites
3q1xA

R

H

Q

Q

T

C

Y

V

L

M

D

A

I

I

I

L

A

E

D

K

Q

L

P

P

S

S

I

I

G

K

E

R

A

T

F

L

R

K

A

T

D

D

I

L

I

I

A

A

V

A

V

Y

E

A

R

G

D

D

P

P

A

A

C

A

T

P

R

G

I

L

I

S

E

L

P

I

V

I

F

R

Y

C

F

Y

K

P

G

G

F

F

Q

Q

A

A

I

V

Q

I

T

V

H

Y

R

R

L

I

A

A

H

H

A

V

L

L

W

Y

S

E

A

C

G

G

R

E

R

R

D

Y

F

Y

A

C

L

R

Y

E

L

M

Q

M

S

E

R

S

S

V

S

H

E

S

V

Y

F

T

Q

S

T

I

D

D

I

I

H

H

P

P

A

G

A

A

R

S

I

I

G

K

K

G

G

H

I

F

F

F

L

I

D

D

H

H

A

G

T

V

G

G

L

V

V

V

I

I

G

G

A

E

T

T

A

A

V

I

I

I

D

G

D

E

D

W

V

C

S

R

M

I

L

Y

Q

Q

D

S

V

V

T

T

L

L

-

G

-

A

-

M

-
x
H

-

F

-

Q

-

E

-

E

G

G

G

G

T

V

G

I

K

K

E

R

K

G

G

T

D

K

R
|
R

H
|
H

P

P

K

T

V

V

R

G

S

D

G

Y

V

V

L

T

I

I

G

G

A

T

G

G

A

A

Q

K

I

V

L

L

G

G

N

N

I

I

E

I

I

V

G

G

R

S

C

H

S

V

R

R

I

I

A

G

A

A

G

N

S

C

V

W

V

I

L

D

K

R

P

D

V

V

P

D

D

S

H

N

K

Q

T

T

V

V

binding serine: H208 (vs. gap), R222 (= R202), H223 (= H203)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
35% identity, 62% coverage: 86:256/277 of query aligns to 98:270/280 of 7bw9A

query
sites
7bw9A

S

T

I

I

G

R

E

E

A

L

F

V

R

K

A

Q

D

D

I

I

I

I

A

A

V

A

V

Y

E

T

R

G

D
|
D

P
|
P

A

A

C

A

T

S

R

S

I

L

I

A

E

M

P

I

V

I

F

R

Y

S

F

Y

K

P

G

G

F

I

Q

H

A

V

I

M

Q

M

T

I

H

Q

R

R

L

V

A

A

H

H

A

I

L

L

W

Y

S

M

A

N

G

G

R

D

R

I

D

E

F

Y

A

S

L

R

Y

E

L

L

Q

M

S

E

R

N

S

I

S

H

E

S

V

V

F

T

Q

G

T

I

D

D

I

I

H

H

P

P

A

G

A

T

R

S

I

I

G

G

K

N

G

H

I

F

F

F

L

I

D
|
D

H
|
H

A

G

T

V

G

G

L

V

V

V

I

I

G

G

A

E

T

T

A

A

V

V

I

I

D

G

D

N

D

W

V

C

S

R

M

V

L

Y

Q

Q

D

S

V

V

T

T

L

L

G

G

G
x
A

T

M

G

S

K

F

E

K

K

G

G

N

D

K

R
|
R

H
|
H

P

P

K

T

V

I

R

G

S

D

G

F

V

V

L

V

I

I

G

G

A

A

G

G

A

A

Q

K

I

V

L

L

G

G

N

N

I

I

E

T

I

I

G

G

R

S

C

N

S

V

R

K

I

I

A

G

A

A

G

N

S

C

V

W

V

I

L

T

K

Q

P

N

V

I

P

D

D

Q

H

D

K

Q

T

I

V

V

-

F

-

I

A

S

G

E

V

H

P

P

A

S

K

Q

I

I

V

T

G

K

E

E

binding cysteine: D114 (= D102), P115 (= P103), D179 (= D167), H180 (= H168), A206 (≠ G194), R214 (= R202), H215 (= H203)

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
30% identity, 36% coverage: 171:269/277 of query aligns to 52:156/294 of 4mzuF

query
sites
4mzuF

G

N

L

V

V

T

I

I

G

K

A

S

T

G

A

V

V

Q

I

I

D

W

D

D

D

G

V

I

S

H

M

I

L

Q

Q

D

D

D

V

V

T

F

L

I

G

G

G

P

T

N

G

V

K

T

E

F

K

T

G
x
N

D
|
D

R
x
K

H

Q

P

P

K

R

-

S

-

K

-

I

-

K

-

T

-

I

V

V

R

K

S

K

G

G

V

A

L

S

I

I

G
|
G

A
|
A

G

N

A

S

Q

T

I

I

L
|
L

G

P

N

G

I

I

E

L

I

I

G

G

R

E

C

N

S

A

R

M

I

V

A
x
G

A
|
A

G

G

S

A

V

V

V

I

L
x
T

K
|
K

P

N

V

V

P

P

D

D

H

N

K

A

T

I

V

V

A
x
I

G

G

V
x
N

P

P

A

G

K
x
R

I

I

V

T

G

G

E

Y

A

V

G

E

C

A

A

F

E

E

P

L

S

K

R

L

L

Q

M

M

D

S

Q

K

I

V

binding coenzyme a: N81 (≠ G200), D82 (= D201), K83 (≠ R202), G100 (= G213), A101 (= A214), L106 (= L219), G118 (≠ A231), A119 (= A232), T124 (≠ L237), K125 (= K238), I134 (≠ A247), N136 (≠ V249), R139 (≠ K252)

Sites not aligning to the query:

binding thymine: 20, 21, 38
binding thymidine-5'-diphosphate: 189, 203, 205, 270

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
36% identity, 27% coverage: 180:255/277 of query aligns to 61:141/290 of 4mzuB

query
sites
4mzuB

I

I

D

W

D

D

D

G

V

I

S

H

M

I

L

Q

Q

D

D

D

V

V

T
x
F

L

I

G

G

G

P

T
x
N

G

V

K

T

E

F

K
x
T

G
x
N

D
|
D

R
x
K

H

Q

P
|
P

K

R

-

S

-

L

-

K

-

T

-

I

V

V

R

K

S

K

G

G

V

A

L

S

I

I

G
|
G

A
|
A

G

N

A

S

Q

T

I

I

L

L

G

P

N

G

I

I

E

L

I

I

G

G

R

E

C

N

S

A

R

M

I

V

A
x
G

A
|
A

G

G

S

A

V

V

V

I

L
x
T

K
|
K

P

N

V

V

P

P

D

D

H

N

K

A

T

I

V

V

A
x
I

G

G

V

N

P

P

A

G

K
x
R

I

I

V

T

G

G

binding coenzyme a: F72 (≠ T191), T80 (≠ K199), N81 (≠ G200), D82 (= D201), K83 (≠ R202), P85 (= P204), G99 (= G213), A100 (= A214), G117 (≠ A231), A118 (= A232), T123 (≠ L237), K124 (= K238), I133 (≠ A247), R138 (≠ K252)
binding magnesium ion: N76 (≠ T195)

Sites not aligning to the query:

binding coenzyme a: 199
binding magnesium ion: 58
binding thymine: 20, 21, 38, 39
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
29% identity, 39% coverage: 150:256/277 of query aligns to 72:184/203 of P07464

query
sites
P07464

F

Y

Q

G

T

S

D

N

I

I

H

H

P

-

A

-

A

-

R

-

I

I

G

G

K

R

G

N

I

F

F

Y

L

A

D
x
N

H

F

A

N

T

L

G

T

L

I

V

V

I

D

G
x
D

A

Y

T

T

A

V

V

T

I

I

D

G

D

D

D

N

V

V

S

L

M

I

L

A

Q

P

D

N

V

V

T

T

L

L

G

S

G

V

T

T

G

G

-
x
H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

K

N

G

G

D

E

R

M

H

Y

P

S

-

F

-

P

-

I

K

T

V

I

R

G

S

N

G

N

V

V

L

W

I

I

G

G

A
x
S

G

H

A

V

Q

V

I

I

L

N

G

P

N

G

I

V

E

T

I

I

G

G

R

D

C

N

S

S

R

V

I

I

A

G

A
|
A

G

G

S

S

V

I

V

V

L
x
T

K
|
K

P

D

V

I

P

P

D

P

H

N

K

V

T

V

V

A

A

A

G

G

V

V

P

P

A

C

K
x
R

I

V

V

I

G
x
R

E

E

N85 (≠ D167) binding in other chain; binding in other chain
D93 (≠ G175) binding substrate
H115 (vs. gap) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A214) binding in other chain
A160 (= A232) binding in other chain
TK 165:166 (≠ LK 237:238) binding acetyl-CoA
R180 (≠ K252) binding acetyl-CoA
R183 (≠ G255) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding substrate
71 binding substrate

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
29% identity, 39% coverage: 150:256/277 of query aligns to 71:183/201 of 1krvA

query
sites
1krvA

F

Y

Q

G

T

S

D

N

I

I

H

H

P

-

A

-

A

-

R

-

I

I

G

G

K

R

G

N

I

F

F
x
Y

L

A

D
x
N

H

F

A

N

T

L

G

T

L

I

V
|
V

I

D

G
x
D

A

Y

T

T

A

V

V

T

I

I

D

G

D

D

D

N

V

V

S

L

M

I

L
x
A

Q

P

D

N

V

V

T

T

L

L

G
x
S

G

V

T
|
T

G

G

-

H

-

P

-

V

-

H

-

H

-

E

-

L

K

R

E

K

K

N

G

G

D

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

K

T

V

I

R

G

S

N

G

N

V

V

L
x
W

I

I

G
|
G

A
x
S

G

H

A

V

Q

V

I

I

L
x
N

G

P

N

G

I

V

E

T

I

I

G

G

R

D

C

N

S

S

R

V

I

I

A

G

A
|
A

G

G

S

S

V

I

V

V

L

T

K

K

P

D

V

I

P

P

D

P

H

N

K

V

T

V

V

A

A

A

G

G

V

V

P
|
P

A

C

K
x
R

I

V

V

I

G
x
R

E

E

binding 4-nitrophenyl beta-D-galactopyranoside: Y82 (≠ F165), N84 (≠ D167), V90 (= V173), D92 (≠ G175), M126 (≠ R202)
binding coenzyme a: A104 (≠ L187), S110 (≠ G193), T112 (= T195), W138 (≠ L211), G140 (= G213), S141 (≠ A214), N146 (≠ L219), A159 (= A232), P177 (= P250), R179 (≠ K252), R182 (≠ G255)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70

Query Sequence

>WP_051952941.1 NCBI__GCF_000746085.1:WP_051952941.1
MTYVRGAAKIVDPAAIDPIFARLRNEAQEVVQNEPELAGAIFSTILGQGTLEAAIIHRIA
TRLGHQAAPADLIRQTYLDIIADQPSIGEAFRADIIAVVERDPACTRIIEPVFYFKGFQA
IQTHRLAHALWSAGRRDFALYLQSRSSEVFQTDIHPAARIGKGIFLDHATGLVIGATAVI
DDDVSMLQDVTLGGTGKEKGDRHPKVRSGVLIGAGAQILGNIEIGRCSRIAAGSVVLKPV
PDHKTVAGVPAKIVGEAGCAEPSRLMDQIIADGPIKK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory