SitesBLAST
Comparing WP_051953766.1 NCBI__GCF_000745855.1:WP_051953766.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
44% identity, 93% coverage: 15:653/686 of query aligns to 10:654/692 of 6iunB
- active site: A60 (≠ S65), F65 (= F70), E73 (≠ D76), H77 (≠ P80), G101 (= G104), E104 (= E107), E124 (= E127), G132 (= G135), K248 (≠ Y247), S407 (= S405), H428 (= H426), E440 (= E438), N478 (= N476)
- binding nicotinamide-adenine-dinucleotide: G300 (= G298), T301 (= T299), M302 (= M300), E321 (= E319), T322 (≠ Q320), Y365 (≠ W363), A377 (= A375), V378 (= V376), E380 (= E378), V384 (≠ I382), V388 (= V386), N405 (= N403), S407 (= S405)
Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 5 papers)
39% identity, 94% coverage: 17:661/686 of query aligns to 13:679/723 of Q08426
- V40 (≠ E44) to G: in dbSNP:rs1062551
- I41 (≠ A45) to R: in dbSNP:rs1062552
- T75 (≠ Q78) to I: in dbSNP:rs1062553
- K165 (≠ V168) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-171; Q-346 and Q-584.
- K171 (≠ A174) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-346 and Q-584.
- A274 (= A271) to T: in dbSNP:rs2302819
- A325 (≠ L317) to G: in dbSNP:rs1062555
- K346 (≠ S338) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-584.
- K584 (≠ E567) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-346.
- K598 (≠ A580) to T: in dbSNP:rs1042437
- T606 (≠ A588) to P: in dbSNP:rs1042438
Sites not aligning to the query:
- 3 E → K: in FRTS3; the mutant is mistargeted to mitochondria; results in impaired mitochondrial oxidative phosphorylation and defects in the transport of fluids across the epithelium of renal proximal tubular cells; dbSNP:rs398124646
6z5oAAA Peroxisomal bifunctional enzyme (see paper)
39% identity, 94% coverage: 17:661/686 of query aligns to 19:677/716 of 6z5oAAA
- active site: A67 (≠ S65), F72 (= F70), G82 (≠ P80), G106 (= G104), E109 (= E107), P128 (= P126), E129 (= E127), G137 (= G135), K255 (≠ Y247), S409 (= S405), H430 (= H426), E442 (= E438), N480 (= N476)
- binding coenzyme a: P26 (= P24), V27 (≠ I25), A65 (= A63), D68 (= D66), I69 (≠ L67), P128 (= P126), Y162 (≠ R160), F277 (= F269), K281 (≠ R273)
- binding nicotinamide-adenine-dinucleotide: G309 (= G296), G311 (= G298), T312 (= T299), M313 (= M300), E332 (= E319), S333 (≠ Q320), Q337 (≠ A324), A379 (= A375), V380 (= V376), F381 (= F377), E382 (= E378), K387 (= K383), N407 (= N403), S409 (= S405), H430 (= H426)
- binding nicotinamide: A67 (≠ S65), E109 (= E107), E129 (= E127), P136 (= P134), F261 (= F253)
3zw9A Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with (2s,3s)-3-hydroxy-2-methylbutanoyl-coa (see paper)
39% identity, 94% coverage: 17:661/686 of query aligns to 16:681/723 of 3zw9A
- active site: A64 (≠ S65), F69 (= F70), G79 (≠ P80), G103 (= G104), E106 (= E107), P125 (= P126), E126 (= E127), P133 (= P134), G134 (= G135), K252 (≠ Y247), S413 (= S405), H434 (= H426), E446 (= E438), N484 (= N476)
- binding nicotinamide-adenine-dinucleotide: L305 (≠ I295), G306 (= G296), G308 (= G298), T309 (= T299), M310 (= M300), E329 (= E319), Q334 (≠ A324), A383 (= A375), V384 (= V376), F385 (= F377), E386 (= E378), N411 (= N403), S413 (= S405), H434 (= H426)
- binding (2s,3s)-3-hydroxy-2-methylbutanoyl-coa: V24 (≠ I25), A62 (= A63), G63 (= G64), A64 (≠ S65), I66 (≠ L67), G102 (= G103), G103 (= G104), E106 (= E107), E126 (= E127), P133 (= P134), Y159 (≠ R160)
5omoA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with with 3s-hydroxy-decanoyl-coa and 3-keto- decanoyl-coa
39% identity, 94% coverage: 17:661/686 of query aligns to 18:683/725 of 5omoA
- active site: A66 (≠ S65), F71 (= F70), G81 (≠ P80), G105 (= G104), E108 (= E107), P127 (= P126), E128 (= E127), P135 (= P134), G136 (= G135), K254 (≠ Y247), S415 (= S405), H436 (= H426), E448 (= E438), N486 (= N476)
- binding (s)-3-hydroxydecanoyl-coa: P25 (= P24), V26 (≠ I25), A28 (= A27), P31 (≠ A30), A64 (= A63), A66 (≠ S65), D67 (= D66), I68 (≠ L67), L103 (= L102), G105 (= G104), E108 (= E107), P127 (= P126), E128 (= E127), Y161 (≠ R160), F260 (= F253), K280 (≠ R273)
- binding 3-keto-decanoyl-coa: S415 (= S405), N486 (= N476), K519 (≠ A509), M520 (= M510), V525 (= V515), Y658 (= Y636)
5mgbA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and NAD (see paper)
39% identity, 94% coverage: 17:661/686 of query aligns to 18:683/725 of 5mgbA
- active site: A66 (≠ S65), F71 (= F70), G81 (≠ P80), G105 (= G104), E108 (= E107), P127 (= P126), E128 (= E127), P135 (= P134), G136 (= G135), K254 (≠ Y247), S415 (= S405), H436 (= H426), E448 (= E438), N486 (= N476)
- binding acetoacetyl-coenzyme a: P25 (= P24), V26 (≠ I25), A64 (= A63), G65 (= G64), A66 (≠ S65), D67 (= D66), I68 (≠ L67), G105 (= G104), E128 (= E127), Y161 (≠ R160)
- binding nicotinamide-adenine-dinucleotide: L307 (≠ I295), G308 (= G296), G310 (= G298), T311 (= T299), M312 (= M300), E331 (= E319), S332 (≠ Q320), Q336 (≠ A324), V386 (= V376), F387 (= F377), E388 (= E378), N413 (= N403), S415 (= S405), H436 (= H426)
3zwcA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-decanoyl-coa (see paper)
39% identity, 94% coverage: 17:661/686 of query aligns to 18:683/725 of 3zwcA