SitesBLAST
Comparing WP_052383965.1 NCBI__GCF_000759345.1:WP_052383965.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
A0QV09 Aldo-keto reductase MSMEG_2407/MSMEI_2346; AKR; AKR5H1; EC 1.1.1.- from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
39% identity, 93% coverage: 2:249/268 of query aligns to 19:267/283 of A0QV09
- G196 (≠ F178) binding NADPH
- L198 (≠ M180) binding NADPH
- V200 (≠ M182) binding NADPH
- I236 (≠ L218) binding NADPH
- R238 (= R220) binding NADPH
- S239 (= S221) binding NADPH
- A240 (≠ T222) binding NADPH
- R244 (= R226) binding NADPH
- S247 (≠ E229) binding NADPH
- N248 (= N230) binding NADPH
Sites not aligning to the query:
2wzmA Crystal structure of a mycobacterium aldo-keto reductase in its apo and liganded form (see paper)
39% identity, 93% coverage: 2:249/268 of query aligns to 10:258/274 of 2wzmA
- active site: D44 (= D36), Y49 (= Y41), K74 (= K66), H107 (= H99)
- binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4s)-3,4-dihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: Y186 (≠ F177), G187 (≠ F178), P188 (≠ V179), L189 (≠ M180), G190 (≠ A181), V191 (≠ M182), G192 (= G183), L195 (≠ P186), A212 (= A203), I227 (≠ L218), R229 (= R220), S230 (= S221), R235 (= R226), N239 (= N230)
Sites not aligning to the query:
1vbjA The crystal structure of prostaglandin f synthase from trypanosoma brucei
35% identity, 96% coverage: 5:260/268 of query aligns to 20:273/281 of 1vbjA
- active site: D52 (= D36), Y57 (= Y41), K82 (= K66), H115 (= H99)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G26 (= G11), M27 (≠ L12), W28 (≠ D13), D52 (= D36), Y57 (= Y41), H115 (= H99), N145 (= N130), Q166 (= Q151), W192 (≠ F177), S193 (≠ F178), P194 (≠ V179), L195 (≠ M180), Q197 (≠ M182), G198 (= G183), V201 (≠ P186), A218 (= A203), I233 (≠ L218), K235 (≠ R220), S236 (= S221), G237 (≠ T222), R241 (= R226), E244 (= E229), N245 (= N230)
A0QV10 Aldo-keto reductase MSMEG_2408/MSMEI_2347; EC 1.1.1.- from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
36% identity, 96% coverage: 3:260/268 of query aligns to 11:267/275 of A0QV10
- K262 (≠ G254) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
Q9GV41 9,11-endoperoxide prostaglandin H2 reductase; Prostaglandin F2-alpha synthase; EC 1.1.1.- from Trypanosoma brucei brucei
35% identity, 96% coverage: 5:260/268 of query aligns to 15:268/276 of Q9GV41
4gieA Crystal structure of prostaglandin f synthase from trypanosoma cruzi bound to NADP (see paper)
35% identity, 97% coverage: 5:264/268 of query aligns to 23:285/288 of 4gieA
- active site: D55 (= D36), Y60 (= Y41), K85 (= K66), H118 (= H99)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G29 (= G11), W31 (≠ D13), D55 (= D36), Y60 (= Y41), H118 (= H99), W119 (= W100), N148 (= N130), Q169 (= Q151), W195 (≠ F177), S196 (≠ F178), P197 (≠ V179), L198 (≠ M180), S200 (≠ M182), L207 (≠ P186), A224 (= A203), I239 (≠ L218), P240 (≠ T219), K241 (≠ R220), S242 (= S221), R247 (= R226), E250 (= E229), N251 (= N230)
4fziA Crystal structure of prostaglandin f synthase from trypanosoma cruzi (see paper)
35% identity, 97% coverage: 5:264/268 of query aligns to 12:274/277 of 4fziA
3wbwA Crystal structure of gox0644 in complex with NADPH
32% identity, 97% coverage: 3:263/268 of query aligns to 12:269/271 of 3wbwA
- active site: D45 (= D36), Y50 (= Y41), K71 (= K66), H104 (= H99)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G20 (= G11), H104 (= H99), N136 (= N130), W183 (≠ F177), R184 (≠ F178), P185 (≠ V179), L186 (≠ M180), L192 (≠ P186), A209 (= A203), K226 (≠ R220), S227 (= S221), V228 (≠ T222), R232 (= R226), E235 (= E229), N236 (= N230)
3d3fA Crystal structure of yvgn and cofactor NADPH from bacillus subtilis (see paper)
35% identity, 97% coverage: 4:262/268 of query aligns to 15:273/275 of 3d3fA
- active site: D48 (= D36), Y53 (= Y41), K78 (= K66), H111 (= H99)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G22 (= G11), F24 (≠ D13), D48 (= D36), Y53 (= Y41), H111 (= H99), S140 (= S129), N141 (= N130), Q162 (= Q151), W188 (≠ F177), S189 (≠ F178), P190 (≠ V179), L191 (≠ M180), Q193 (≠ M182), L197 (≠ P186), I229 (≠ L218), K231 (≠ R220), S232 (= S221), K234 (≠ R223), R237 (= R226), E240 (= E229), N241 (= N230)
4g5dA X-ray crystal structure of prostaglandin f synthase from leishmania major friedlin bound to NADPH (see paper)
33% identity, 92% coverage: 4:249/268 of query aligns to 15:268/283 of 4g5dA
- active site: D48 (= D36), Y53 (= Y41), K78 (= K66), H111 (= H99)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G22 (= G11), V23 (≠ L12), W24 (vs. gap), D48 (= D36), Y53 (= Y41), H111 (= H99), S148 (= S129), N149 (= N130), Q170 (= Q151), W196 (≠ F177), S197 (≠ F178), P198 (≠ V179), L199 (≠ M180), Q201 (≠ M182), G202 (= G183), L205 (≠ P186), I237 (≠ L218), P238 (≠ T219), K239 (≠ R220), S240 (= S221), V241 (≠ T222), H242 (≠ R223), R245 (= R226), E248 (= E229), N249 (= N230)
H9JTG9 Aldo-keto reductase AKR2E4; 3-dehydroecdysone reductase; Aldo-keto reductase 2E; EC 1.1.1.- from Bombyx mori (Silk moth) (see paper)
34% identity, 84% coverage: 27:252/268 of query aligns to 44:291/308 of H9JTG9
- D53 (= D36) binding NADP(+)
- SN 158:159 (= SN 129:130) binding NADP(+)
- R215 (≠ M182) binding NADP(+)
- 259:269 (vs. 220:230, 55% identical) binding NADP(+)
Sites not aligning to the query:
3wczA Crystal structure of bombyx mori aldo-keto reductase (akr2e4) in complex with NADP (see paper)
34% identity, 84% coverage: 27:252/268 of query aligns to 43:290/307 of 3wczA
- active site: D52 (= D36), Y57 (= Y41), K86 (= K66), H119 (= H99)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: D52 (= D36), Y57 (= Y41), H119 (= H99), Q179 (= Q151), Y205 (≠ F177), S206 (vs. gap), P207 (vs. gap), F208 (vs. gap), R214 (≠ M182), I256 (≠ L218), P257 (≠ T219), K258 (≠ R220), S259 (= S221), T260 (= T222), R264 (= R226), N268 (= N230)
Sites not aligning to the query:
1a80A Native 2,5-diketo-d-gluconic acid reductase a from corynbacterium sp. Complexed with NADPH (see paper)
38% identity, 92% coverage: 3:249/268 of query aligns to 11:260/277 of 1a80A
- active site: D44 (= D36), Y49 (= Y41), K74 (= K66), H107 (= H99)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G19 (= G11), F21 (≠ D13), D44 (= D36), Y49 (= Y41), H107 (= H99), S138 (= S129), Q160 (= Q151), W186 (vs. gap), G187 (vs. gap), P188 (= P173), L189 (= L174), Q191 (≠ G176), A214 (= A203), F229 (≠ L218), K231 (≠ R220), S232 (= S221), V233 (≠ T222), R234 (= R223), R237 (= R226), E240 (= E229), N241 (= N230)
P06632 2,5-diketo-D-gluconic acid reductase A; 2,5-DKG reductase A; 2,5-DKGR A; 25DKGR-A; AKR5C; EC 1.1.1.346 from Corynebacterium sp. (strain ATCC 31090) (see 3 papers)
38% identity, 92% coverage: 3:249/268 of query aligns to 12:261/278 of P06632
- Y50 (= Y41) active site, Proton donor
- H108 (= H99) binding substrate
- 188:242 (vs. 173:230, 42% identical) binding NADP(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3b3dA B.Subtilis ytbe (see paper)
34% identity, 97% coverage: 4:262/268 of query aligns to 16:278/280 of 3b3dA
P17516 Aldo-keto reductase family 1 member C4; 3-alpha-hydroxysteroid dehydrogenase type I; 3-alpha-HSD1; 3alpha-hydroxysteroid 3-dehydrogenase; Chlordecone reductase; CDR; Dihydrodiol dehydrogenase 4; DD-4; DD4; HAKRA; EC 1.1.1.-; EC 1.1.1.209; EC 1.1.1.210; EC 1.1.1.51; EC 1.1.1.53; EC 1.1.1.62; EC 1.1.1.357; EC 1.1.1.225 from Homo sapiens (Human) (see 9 papers)
31% identity, 93% coverage: 5:252/268 of query aligns to 16:302/323 of P17516
- GFGTY 20:24 (≠ GFGL- 9:12) binding NADP(+)
- D50 (= D36) binding NADP(+)
- S145 (vs. gap) to C: in dbSNP:rs3829125
- SN 166:167 (= SN 129:130) binding NADP(+)
- C170 (≠ A133) to Y: in dbSNP:rs17851824
- Q190 (= Q151) binding NADP(+)