SitesBLAST
Comparing WP_052384180.1 NCBI__GCF_000759345.1:WP_052384180.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4kugA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with NAD from clostridium butyricum
40% identity, 56% coverage: 13:289/498 of query aligns to 6:281/282 of 4kugA
- active site: S117 (= S124), H138 (= H145), E150 (= E157), N188 (= N195)
- binding nicotinamide-adenine-dinucleotide: G10 (= G17), T11 (≠ A18), M12 (= M19), R30 (≠ H37), D31 (= D38), A87 (= A94), A88 (≠ I95), E90 (= E97), N115 (= N122), S117 (= S124), H138 (= H145)
4kuhA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with acetoacetyl-coa from clostridium butyricum
40% identity, 55% coverage: 13:288/498 of query aligns to 6:280/280 of 4kuhA
6aa8E Crystal structure of (s)-3-hydroxybutyryl-coenzymea dehydrogenase from clostridium acetobutylicum complexed with NAD+ (see paper)
40% identity, 56% coverage: 13:289/498 of query aligns to 5:280/281 of 6aa8E
- active site: S116 (= S124), H137 (= H145), E149 (= E157), N187 (= N195)
- binding nicotinamide-adenine-dinucleotide: I6 (≠ V14), G9 (= G17), T10 (≠ A18), M11 (= M19), R29 (≠ H37), D30 (= D38), I31 (≠ A39), A86 (= A94), A87 (≠ I95), E89 (= E97), K94 (= K102), N114 (= N122), S116 (= S124)
Q16836 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Homo sapiens (Human) (see 7 papers)
40% identity, 55% coverage: 13:288/498 of query aligns to 32:313/314 of Q16836
- GGGLMG 34:39 (≠ GAGAMG 15:20) binding NAD(+)
- A40 (≠ G21) to T: in HADH deficiency; dbSNP:rs137853101
- D57 (= D38) binding NAD(+); to E: in HADH deficiency; dbSNP:rs137853102; to G: found in a patient with Reye-like syndrome; does not affect 3-hydroxyacyl-CoA dehydrogenase activity; increases KM value for NADH; does not affect dimerization
- S73 (vs. gap) binding CoA
- K80 (≠ D53) binding CoA
- L86 (≠ V59) to P: in dbSNP:rs4956145
- E122 (= E97) binding NAD(+)
- K127 (= K102) binding NAD(+); modified: N6-(2-hydroxyisobutyryl)lysine
- S149 (= S124) binding CoA; binding NAD(+)
- Q152 (≠ P127) to H: in dbSNP:rs1051519
- N173 (= N148) binding NAD(+)
- Y226 (= Y201) to H: found in a patient with Reye-like syndrome; loss of 3-hydroxyacyl-CoA dehydrogenase activity. Does not affect dimerization; dbSNP:rs146036912
- P258 (= P233) to L: in HHF4; loss of 3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137853103
- K305 (= K280) binding NAD(+)
P00348 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; L-3-hydroxyacyl CoA dehydrogenase; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Sus scrofa (Pig) (see paper)
39% identity, 55% coverage: 13:288/498 of query aligns to 32:313/314 of P00348
- GGGLMG 34:39 (≠ GAGAMG 15:20) binding NAD(+)
- D57 (= D38) binding NAD(+)
- E122 (= E97) binding NAD(+)
- K127 (= K102) binding NAD(+)
- S149 (= S124) binding NAD(+)
1f17A L-3-hydroxyacyl-coa dehydrogenase complexed with nadh (see paper)
40% identity, 55% coverage: 13:288/498 of query aligns to 9:290/293 of 1f17A
- active site: S126 (= S124), H147 (= H145), E159 (= E157), N197 (= N195)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G13 (= G17), L14 (≠ A18), M15 (= M19), D34 (= D38), Q35 (≠ A39), A96 (= A94), I97 (= I95), E99 (= E97), K104 (= K102), N124 (= N122), S126 (= S124), H147 (= H145)
1f12A L-3-hydroxyacyl-coa dehydrogenase complexed with 3-hydroxybutyryl-coa (see paper)
40% identity, 55% coverage: 13:288/498 of query aligns to 9:290/293 of 1f12A
- active site: S126 (= S124), H147 (= H145), E159 (= E157), N197 (= N195)
- binding 3-hydroxybutanoyl-coenzyme a: K57 (≠ D53), S126 (= S124), H147 (= H145), F149 (= F147), N150 (= N148), M155 (= M153), N197 (= N195), L200 (≠ G198), P232 (≠ R230), M233 (= M231), L238 (= L236)
1f0yA L-3-hydroxyacyl-coa dehydrogenase complexed with acetoacetyl-coa and NAD+ (see paper)
40% identity, 55% coverage: 13:288/498 of query aligns to 9:290/291 of 1f0yA
- active site: S126 (= S124), H147 (= H145), E159 (= E157), N197 (= N195)
- binding acetoacetyl-coenzyme a: S50 (vs. gap), K57 (≠ D53), S126 (= S124), H147 (= H145), F149 (= F147), N150 (= N148), P151 (= P149), P153 (= P151), V154 (≠ A152), N197 (= N195), P232 (≠ R230), M233 (= M231), L238 (= L236)
- binding nicotinamide-adenine-dinucleotide: G13 (= G17), L14 (≠ A18), M15 (= M19), D34 (= D38), Q35 (≠ A39), A96 (= A94), I97 (= I95), E99 (= E97), K104 (= K102), N124 (= N122), S126 (= S124), H147 (= H145), N150 (= N148), V242 (≠ T240), T246 (≠ V244), K282 (= K280)
4pzeA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with acetoacetyl-coa (see paper)
37% identity, 56% coverage: 10:289/498 of query aligns to 4:282/283 of 4pzeA
4pzdA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with NAD+ (see paper)
37% identity, 56% coverage: 10:289/498 of query aligns to 4:282/283 of 4pzdA
- active site: S118 (= S124), H139 (= H145), E151 (= E157), N189 (= N195)
- binding nicotinamide-adenine-dinucleotide: G11 (= G17), T12 (≠ A18), M13 (= M19), D32 (= D38), A88 (= A94), A89 (≠ I95), T90 (≠ V96), E91 (= E97), I99 (≠ L105), N116 (= N122), S118 (= S124), N142 (= N148)
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
34% identity, 62% coverage: 13:320/498 of query aligns to 296:594/692 of 6iunB
- active site: S407 (= S124), H428 (= H145), E440 (= E157), N478 (= N195)
- binding nicotinamide-adenine-dinucleotide: G300 (= G17), T301 (≠ A18), M302 (= M19), E321 (≠ D38), T322 (≠ A39), Y365 (≠ L82), A377 (= A94), V378 (≠ I95), E380 (= E97), V384 (= V101), V388 (≠ L105), N405 (= N122), S407 (= S124)
Sites not aligning to the query:
P9WNP7 3-hydroxybutyryl-CoA dehydrogenase; Beta-hydroxybutyryl-CoA dehydrogenase; BHBD; EC 1.1.1.157 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
39% identity, 56% coverage: 11:288/498 of query aligns to 8:286/286 of P9WNP7
- S122 (= S124) mutation to A: Loss of fatty acyl dehydrogenase activity.
P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
34% identity, 59% coverage: 11:303/498 of query aligns to 364:654/763 of P40939
- E510 (= E157) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769
Sites not aligning to the query:
- 282 V → D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
- 305 I → N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
- 342 L → P: in LCHAD deficiency; dbSNP:rs137852772
8oqqA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-79 (see paper)
35% identity, 56% coverage: 11:291/498 of query aligns to 331:613/723 of 8oqqA
Sites not aligning to the query:
8oqpA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-76 (see paper)
35% identity, 56% coverage: 11:291/498 of query aligns to 331:613/723 of 8oqpA
Sites not aligning to the query:
- binding 2-azanyl-5-sulfo-benzoic acid: 28, 29, 63, 64, 64, 65, 143, 144, 147, 307, 661, 662, 674, 698, 705
8oqvA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-109 (see paper)
35% identity, 56% coverage: 11:291/498 of query aligns to 335:617/726 of 8oqvA