SitesBLAST

P02911 Lysine/arginine/ornithine-binding periplasmic protein; LAO-binding protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
34% identity, 100% coverage: 1:255/256 of query aligns to 1:259/260 of P02911

query
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P02911

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D33 (≠ E33) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
Y36 (= Y35) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
D52 (≠ E51) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
C60 (= C59) modified: Disulfide link with 67
C67 (= C66) modified: Disulfide link with 60
F74 (≠ W73) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
S91 (≠ N90) binding L-arginine; binding L-ornithine; mutation to A: Has no effect on the arginine and histidine binding affinity.
S92 (= S91) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
S94 (= S93) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
R99 (= R98) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
L139 (≠ Q137) mutation to K: Changes ligand specificity. Can bind glutamine. Still able to bind basic amino acids, however, with 1000-fold less affinity for arginine.; mutation to Q: Does not affect ligand preference.
T143 (= T141) binding L-arginine; binding L-lysine; binding L-ornithine
D183 (= D181) binding L-arginine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.

4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
35% identity, 88% coverage: 26:250/256 of query aligns to 6:226/226 of 4zv1A

query
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4zv1A

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binding arginine: E11 (≠ S31), F14 (≠ Y35), F52 (≠ W73), A69 (≠ N90), G70 (≠ S91), M71 (≠ L92), T72 (≠ S93), R77 (= R98), Q117 (= Q137), S120 (≠ T140), T121 (= T141), D161 (= D181)

P02910 Histidine-binding periplasmic protein; HBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
38% identity, 90% coverage: 25:255/256 of query aligns to 25:259/260 of P02910

query
sites
P02910

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E40 (≠ T39) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
K42 (= K41) mutation to E: Increases ATPase-inducing activity and histidine transport; when associated with K-47.
E47 (≠ S46) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
D171 (= D169) mutation D->A,N: Strong decrease in ATPase-inducing activity and histidine transport.
R176 (= R174) mutation R->D,S: Strong decrease in ATPase-inducing activity and histidine transport.
D178 (= D176) mutation to A: Slight decrease in ATPase-inducing activity and histidine transport.

Sites not aligning to the query:

1:22 signal peptide

1hslA Refined 1.89 angstroms structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport(slash)chemosensory receptors (see paper)
38% identity, 91% coverage: 23:255/256 of query aligns to 1:237/238 of 1hslA

query
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1hslA

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binding histidine: Y14 (= Y35), L52 (≠ W73), S69 (≠ N90), S70 (= S91), L71 (= L92), S72 (= S93), R77 (= R98), T120 (= T140), T121 (= T141), D161 (= D181)

5owfA Structure of a lao-binding protein mutant with glutamine (see paper)
35% identity, 86% coverage: 35:255/256 of query aligns to 11:234/235 of 5owfA

query
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S

D

D

D

D

T

D

E

L

L

R

K

Q

A

R

A

L

F

N

D

Q

K

A

A

I

L

S

T

E

E

A

L

L

R

E

Q

D

D

G

G

T

T

C

Y

T

D

D

K

L

M

S

A

Q

K

E

K

Y

Y

F

F

G

D

T

F

D

N

I

V

C

Y

G

G

binding glutamine: Y11 (= Y35), F49 (≠ W73), S66 (≠ N90), S67 (= S91), S69 (= S93), R74 (= R98), K114 (≠ Q137), S117 (≠ T140), T118 (= T141), D158 (= D181)

1lstA Three-dimensional structures of the periplasmic lysine-, arginine-, ornithine-binding protein with and without a ligand (see paper)
35% identity, 86% coverage: 35:255/256 of query aligns to 14:237/238 of 1lstA

query
sites
1lstA

Y
|
Y

P

A

P

P

F

F

T

S

F

S

K

K

G

D

S

A

D

K

G

G

S

E

W

F

T

I

G

G

F

F

E

D

V

I

E

D

L

L

G

G

Q

N

A

E

L

M

C

C

E

K

K

R

M

M

Q

Q

A

V

E

K

C

C

E

T

I

W

T

V

P

A

T

S

G

D

W
x
F

S

D

G

A

I

L

F

I

A

P

A

S

L

L

D

K

S

A

G

K

K

K

I

I

D

D

M

A

I

I

M

I

N

S

S
|
S

L

L

S
|
S

I

I

T

T

E

D

Q

K

R
|
R

K

Q

E

Q

V

E

I

I

D

A

F

F

T

S

D

D

P

K

Y

L

Y

Y

F

A

T

A

P

D

S

S

A

R

Y

L

V

I

A

A

K

K

S

G

D

S

S

P

F

I

D

Q

-

P

I

T

P

L

E

E

G

S

L

L

D

K

G

G

K

K

L

H

L

V

G

G

V

V

Q
x
L

G

Q

G

G

T
x
S

T
|
T

N
x
Q

A

E

T

A

Y

Y

A

A

R

N

R

D

E

N

L

W

R

R

D

T

T

K

G

G

V

V

E

D

I

V

K

V

I

A

Y

Y

D

A

Q

N

Q

Q

E

D

Q

L

A

I

N

Y

R

S

D

D

L

L

F

T

A

A

G

G

R

R

V

L

D

D

A

A

I

A

L

L

A

Q

D
|
D

K

E

V

V

A

A

M

A

T

S

E

E

-

G

L

F

V

L

E

K

R

Q

D

P

E

A

A

G

S

K

E

E

Y

Y

E

A

I

F

R

A

A

G

T

P

A

S

P

V

R

K

H

D

A

K

-

Y

F

F

G

G

E

D

G

G

V

T

G

G

I

V

G

G

L

L

R

R

Q

K

S

D

D

D

D

T

D

E

L

L

R

K

Q

A

R

A

L

F

N

D

Q

K

A

A

I

L

S

T

E

E

A

L

L

R

E

Q

D

D

G

G

T

T

C

Y

T

D

D

K

L

M

S

A

Q

K

E

K

Y

Y

F

F

G

D

T

F

D

N

I

V

C

Y

G

G

binding lysine: Y14 (= Y35), F52 (≠ W73), S70 (= S91), S72 (= S93), R77 (= R98), L117 (≠ Q137), S120 (≠ T140), T121 (= T141), Q122 (≠ N142), D161 (= D181)

1lahE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
35% identity, 86% coverage: 35:255/256 of query aligns to 14:237/238 of 1lahE

query
sites
1lahE

Y
|
Y

P

A

P

P

F

F

T

S

F

S

K

K

G

D

S

A

D

K

G

G

S

E

W

F

T

I

G

G

F

F

E

D

V

I

E

D

L

L

G

G

Q

N

A

E

L

M

C

C

E

K

K

R

M

M

Q

Q

A

V

E

K

C

C

E

T

I

W

T

V

P

A

T

S

G

D

W
x
F

S

D

G

A

I

L

F

I

A

P

A

S

L

L

D

K

S

A

G

K

K

K

I

I

D

D

M

A

I

I

M

I

N

S

S
|
S

L

L

S
|
S

I

I

T

T

E

D

Q

K

R
|
R

K

Q

E

Q

V

E

I

I

D

A

F

F

T

S

D

D

P

K

Y

L

Y

Y

F

A

T

A

P

D

S

S

A

R

Y

L

V

I

A

A

K

K

S

G

D

S

S

P

F

I

D

Q

-

P

I

T

P

L

E

E

G

S

L

L

D

K

G

G

K

K

L

H

L

V

G

G

V

V

Q

L

G

Q

G

G

T
x
S

T

T

N
x
Q

A

E

T

A

Y

Y

A

A

R

N

R

D

E

N

L

W

R

R

D

T

T

K

G

G

V

V

E

D

I

V

K

V

I

A

Y

Y

D

A

Q

N

Q

Q

E

D

Q

L

A

I

N

Y

R

S

D

D

L

L

F

T

A

A

G

G

R

R

V

L

D

D

A

A

I

A

L

L

A

Q

D
|
D

K

E

V

V

A

A

M

A

T

S

E

E

-

G

L

F

V

L

E

K

R

Q

D

P

E

A

A

G

S

K

E

E

Y

Y

E

A

I

F

R

A

A

G

T

P

A

S

P

V

R

K

H

D

A

K

-

Y

F

F

G

G

E

D

G

G

V

T

G

G

I

V

G

G

L

L

R

R

Q

K

S

D

D

D

D

T

D

E

L

L

R

K

Q

A

R

A

L

F

N

D

Q

K

A

A

I

L

S

T

E

E

A

L

L

R

E

Q

D

D

G

G

T

T

C

Y

T

D

D

K

L

M

S

A

Q

K

E

K

Y

Y

F

F

G

D

T

F

D

N

I

V

C

Y

G

G

binding L-ornithine: Y14 (= Y35), F52 (≠ W73), S70 (= S91), S72 (= S93), R77 (= R98), S120 (≠ T140), Q122 (≠ N142), D161 (= D181)

1lagE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
35% identity, 86% coverage: 35:255/256 of query aligns to 14:237/238 of 1lagE

query
sites
1lagE

Y
|
Y

P

A

P

P

F

F

T

S

F

S

K

K

G

D

S

A

D

K

G

G

S

E

W

F

T

I

G

G

F

F

E

D

V

I

E

D

L

L

G

G

Q

N

A

E

L

M

C

C

E

K

K

R

M

M

Q

Q

A

V

E

K

C

C

E

T

I

W

T

V

P

A

T

S

G

D

W
x
F

S

D

G

A

I

L

F

I

A

P

A

S

L

L

D

K

S

A

G

K

K

K

I

I

D

D

M

A

I

I

M

I

N
x
S

S
|
S

L
|
L

S
|
S

I

I

T

T

E

D

Q

K

R
|
R

K

Q

E

Q

V

E

I

I

D

A

F

F

T

S

D

D

P

K

Y

L

Y

Y

F

A

T

A

P

D

S

S

A

R

Y

L

V

I

A

A

K

K

S

G

D

S

S

P

F

I

D

Q

-

P

I

T

P

L

E

E

G

S

L

L

D

K

G

G

K

K

L

H

L

V

G

G

V

V

Q
x
L

G

Q

G

G

T
x
S

T

T

N
x
Q

A

E

T

A

Y

Y

A

A

R

N

R

D

E

N

L

W

R

R

D

T

T

K

G

G

V

V

E

D

I

V

K

V

I

A

Y

Y

D

A

Q

N

Q

Q

E

D

Q

L

A

I

N

Y

R

S

D

D

L

L

F

T

A

A

G

G

R

R

V

L

D

D

A

A

I

A

L

L

A

Q

D
|
D

K

E

V

V

A

A

M

A

T

S

E

E

-

G

L

F

V

L

E

K

R

Q

D

P

E

A

A

G

S

K

E

E

Y

Y

E

A

I

F

R

A

A

G

T

P

A

S

P

V

R

K

H

D

A

K

-

Y

F

F

G

G

E

D

G

G

V

T

G

G

I

V

G

G

L

L

R

R

Q

K

S

D

D

D

D

T

D

E

L

L

R

K

Q

A

R

A

L

F

N

D

Q

K

A

A

I

L

S

T

E

E

A

L

L

R

E

Q

D

D

G

G

T

T

C

Y

T

D

D

K

L

M

S

A

Q

K

E

K

Y

Y

F

F

G

D

T

F

D

N

I

V

C

Y

G

G

binding histidine: Y14 (= Y35), F52 (≠ W73), S69 (≠ N90), S70 (= S91), L71 (= L92), S72 (= S93), R77 (= R98), L117 (≠ Q137), S120 (≠ T140), Q122 (≠ N142), D161 (= D181)

1lafE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
35% identity, 86% coverage: 35:255/256 of query aligns to 14:237/238 of 1lafE

query
sites
1lafE

Y
|
Y

P

A

P

P

F

F

T

S

F

S

K

K

G

D

S

A

D

K

G

G

S

E

W

F

T

I

G

G

F

F

E

D

V

I

E

D

L

L

G

G

Q

N

A

E

L

M

C

C

E

K

K

R

M

M

Q

Q

A

V

E

K

C

C

E

T

I

W

T

V

P

A

T

S

G

D

W
x
F

S

D

G

A

I

L

F

I

A

P

A

S

L

L

D

K

S

A

G

K

K

K

I

I

D

D

M

A

I

I

M

I

N
x
S

S
|
S

L

L

S
|
S

I

I

T

T

E

D

Q

K

R
|
R

K

Q

E

Q

V

E

I

I

D

A

F

F

T

S

D

D

P

K

Y

L

Y

Y

F

A

T

A

P

D

S

S

A

R

Y

L

V

I

A

A

K

K

S

G

D

S

S

P

F

I

D

Q

-

P

I

T

P

L

E

E

G

S

L

L

D

K

G

G

K

K

L

H

L

V

G

G

V

V

Q
x
L

G

Q

G

G

T
x
S

T
|
T

N
x
Q

A

E

T

A

Y

Y

A

A

R

N

R

D

E

N

L

W

R

R

D

T

T

K

G

G

V

V

E

D

I

V

K

V

I

A

Y

Y

D

A

Q

N

Q

Q

E

D

Q

L

A

I

N

Y

R

S

D

D

L

L

F

T

A

A

G

G

R

R

V

L

D

D

A

A

I

A

L

L

A

Q

D
|
D

K

E

V

V

A

A

M

A

T

S

E

E

-

G

L

F

V

L

E

K

R

Q

D

P

E

A

A

G

S

K

E

E

Y

Y

E

A

I

F

R

A

A

G

T

P

A

S

P

V

R

K

H

D

A

K

-

Y

F

F

G

G

E

D

G

G

V

T

G

G

I

V

G

G

L

L

R

R

Q

K

S

D

D

D

D

T

D

E

L

L

R

K

Q

A

R

A

L

F

N

D

Q

K

A

A

I

L

S

T

E

E

A

L

L

R

E

Q

D

D

G

G

T

T

C

Y

T

D

D

K

L

M

S

A

Q

K

E

K

Y

Y

F

F

G

D

T

F

D

N

I

V

C

Y

G

G

binding arginine: Y14 (= Y35), F52 (≠ W73), S69 (≠ N90), S70 (= S91), S72 (= S93), R77 (= R98), L117 (≠ Q137), S120 (≠ T140), T121 (= T141), Q122 (≠ N142), D161 (= D181)

Sites not aligning to the query:

binding arginine: 11

4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
34% identity, 88% coverage: 26:250/256 of query aligns to 6:224/225 of 4zv2A

query
sites
4zv2A

L

L

K

R

I

V

G

G

I

T

S
x
E

A

A

E

-

P

T

Y
x
F

P

P

F

F

T

G

F

F

K

K

G

D

S

E

D

N

G

G

S

K

W

L

T

V

G

G

F

F

E

D

V

I

E

D

L

L

G

A

Q

K

A

A

L

I

C

A

E

K

K

K

M

L

Q

G

A

V

E

K

C

V

E

E

I

F

T

K

P

P

T

M

G

D

W
x
F

S

D

G

G

I

I

F

I

A

P

A

A

L

L

D

Q

S

S

G

G

K

K

I

I

D

D

M

V

I

V

M

I

N

A

S
x
G

L
x
M

S
x
T

I

I

T

T

E

E

Q

E

R
|
R

K

K

E

K

V

Q

I

V

D

D

F

F

T

S

D

D

P

P

Y

Y

Y

F

F

E

T

A

P

G

S

Q

A

A

Y

I

V

V

A

V

A

K

K

K

-

G

S

N

D

D

S

S

F

I

D

K

I

S

P

L

E

E

G

D

L

L

D

K

G

G

K

K

L

K

L

V

G

G

V

V

Q

Q

G

L

G

G

T
x
S

T
|
T

N

S

A

E

T

Q

Y

H

A

V

R

K

E

V

L

A

R

-

D

-

T

A

G

G

V

V

E

K

I

V

K

K

I

K

Y

F

D

D

Q

N

Q

F

E

S

Q

E

A

A

N

F

R

Q

D

E

L

L

F

K

A

S

G

G

R

R

V

V

D

D

A

A

I

V

L

V

A

T

D
|
D

K

N

V

A

A

V

M

A

T

L

E

A

L

Y

V

V

E

K

R

Q

D

N

E

P

A

N

S

A

E

G

Y

V

E

K

I

I

R

V

A

G

T

-

A

-

P

-

R

-

H

E

A

T

A

F

F

S

G

G

E

E

G

P

V

Y

G

G

I

I

G

A

L

V

R

R

Q

K

S

G

D

N

D

S

D

E

L

L

R

L

Q

E

R

K

L

I

N

N

Q

K

A

A

I

L

S

E

E

E

A

M

L

K

E

K

D

D

G

G

T

T

C

Y

T

D

D

K

L

I

S

Y

Q

E

E

K

Y

W

F

F

G

G

binding glutamine: E11 (≠ S31), F14 (≠ Y35), F52 (≠ W73), G70 (≠ S91), M71 (≠ L92), T72 (≠ S93), R77 (= R98), S120 (≠ T140), T121 (= T141), D159 (= D181)

5itoA Structure of the periplasmic binding protein m117n-noct from a. Tumefaciens in complex with octopine (see paper)
33% identity, 85% coverage: 35:252/256 of query aligns to 13:252/255 of 5itoA

query
sites
5itoA

Y
|
Y

P

A

P

P

F

Y

T

N

F

F

K

K

G

D

S

A

D

G

G

G

S

K

W

L

T

I

G

G

F

F

E

D

V

I

E

D

L

L

G

G

Q

N

A

D

L

L

C

C

E

K

K

R

M

M

Q

N

A

I

E

E

C

C

E

K

I

F

T

V

P

E

T

Q

G

A

W
|
W

S

D

G

G

I

I

F

I

A

P

A

S

L

L

D

T

S

A

G

G

K

R

I

Y

D

D

M

A

I

I

M

M

N
x
A

S
x
A

L

M

S
x
G

I

I

T

Q

E

P

Q

A

R
|
R

K

E

E

K

V

V

I

I

D

A

F

F

T

S

D

R

P

P

Y

Y

Y

L

F

L

T

T

P

P

S

N

A

T

Y

F

-

L

-

T

-

A

-

D

-

S

-

P

-

L

-

K

-

T

-

Q

V

V

A

A

A

I

K

E

S

N

D

L

S

P

F

L

D

D

-

N

-

I

I

T

P

P

E

E

G

Q

-

K

-

A

-

E

-

L

-

D

-

K

-

F

-

T

-

K

-

I

L

F

D

E

G

G

K

V

L

K

L

F

G

G

V

V

Q
|
Q

G

A

G

G

T
|
T

T
x
S

N
x
H

A

E

T

A

Y

F

A

M

R

K

R

Q

E

M

L

M

R

-

D

-

T

P

G

S

V

V

E

Q

I

I

K

S

I

T

Y

Y

D

D

Q

T

Q

I

E

D

Q

N

A

V

N

V

R

M

D

D

L

L

F

K

A

A

G

G

R

R

V

I

D

D

A

A

I

S

L

L

A

A

D
x
S

K

V

V

S

A

F

M

L

T

K

E

P

L

L

V

T

E

-

R

-

D

D

E

K

A

P

S

D

E

N

Y

K

E

D

I

L

R

K

A

M

T

F

A

G

P

P

R

R

H

M

A

T

A

G

-

G

-

L

F

F

G

G

E

K

G

G

V

V

G

G

I

V

G

G

L

I

R

R

Q

K

S

E

D

D

D

A

D

D

L

L

R

K

Q

A

R

L

L

F

N

D

Q

K

A

A

I

I

S

D

E

A

A

A

L

I

E

A

D

D

G

G

T

T

C

V

T

Q

D

K

L

L

S

S

Q

Q

E

Q

Y

W

F

F

G

G

T

Y

D

D

binding octopine: Y13 (= Y35), W51 (= W73), A68 (≠ N90), A69 (≠ S91), G71 (≠ S93), R76 (= R98), Q139 (= Q137), T142 (= T140), S143 (≠ T141), H144 (≠ N142), S181 (≠ D181)

Sites not aligning to the query:

binding octopine: 10

5otcA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with noroctopinic acid. (see paper)
33% identity, 85% coverage: 35:252/256 of query aligns to 12:251/256 of 5otcA

query
sites
5otcA

Y
|
Y

P

A

P

P

F

Y

T

N

F

F

K

K

G

D

S

A

D

G

G

G

S

K

W

L

T

I

G

G

F

F

E

D

V

I

E

D

L

L

G

G

Q

N

A

D

L

L

C

C

E

K

K

R

M

M

Q

N

A

I

E

E

C

C

E

K

I

F

T

V

P

E

T

Q

G

A

W
|
W

S

D

G

G

I

I

F

I

A

P

A

S

L

L

D

T

S

A

G

G

K

R

I

Y

D

D

M

A

I

I

M

M

N

A

S
x
A

L

M

S
x
G

I

I

T

Q

E

P

Q

A

R
|
R

K

E

E

K

V

V

I

I

D

A

F

F

T

S

D

R

P

P

Y

Y

Y

L

F

L

T

T

P

P

S

M

A

T

Y

F

-

L

-

T

-

A

-

D

-

S

-

P

-

L

-

K

-

T

-

Q

V

V

A

A

A

I

K

E

S

N

D

L

S

P

F

L

D

D

-

N

-

I

I

T

P

P

E

E

G

Q

-

K

-

A

-

E

-

L

-

D

-

K

-

F

-

T

-

K

-

I

L

F

D

E

G

G

K

V

L

K

L

F

G

G

V

V

Q
|
Q

G

A

G

G

T
|
T

T
x
S

N
x
H

A

E

T

A

Y

F

A

M

R

K

R

Q

E

M

L

M

R

-

D

-

T

P

G

S

V

V

E

Q

I

I

K

S

I

T

Y

Y

D

D

Q

T

Q

I

E

D

Q

N

A

V

N

V

R

M

D

D

L

L

F

K

A

A

G

G

R

R

V

I

D

D

A

A

I

S

L

L

A

A

D
x
S

K

V

V

S

A

F

M

L

T

K

E

P

L

L

V

T

E

-

R

-

D

D

E

K

A

P

S

D

E

N

Y

K

E

D

I

L

R

K

A

M

T

F

A

G

P

P

R

R

H

M

A

T

A

G

-

G

-

L

F

F

G

G

E

K

G

G

V

V

G

G

I

V

G

G

L

I

R

R

Q

K

S

E

D

D

D

A

D

D

L

L

R

K

Q

A

R

L

L

F

N

D

Q

K

A

A

I

I

S

D

E

A

A

A

L

I

E

A

D

D

G

G

T

T

C

V

T

Q

D

K

L

L

S

S

Q

Q

E

Q

Y

W

F

F

G

G

T

Y

D

D

binding (2~{S})-5-azanyl-2-(2-hydroxy-2-oxoethylamino)pentanoic acid: Y12 (= Y35), W50 (= W73), A68 (≠ S91), G70 (≠ S93), R75 (= R98), Q138 (= Q137), T141 (= T140), S142 (≠ T141), H143 (≠ N142), S180 (≠ D181)

Sites not aligning to the query:

binding (2~{S})-5-azanyl-2-(2-hydroxy-2-oxoethylamino)pentanoic acid: 9

5otaA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with octopinic acid (see paper)
33% identity, 85% coverage: 35:252/256 of query aligns to 12:251/254 of 5otaA

query
sites
5otaA

Y
|
Y

P

A

P

P

F

Y

T

N

F

F

K

K

G

D

S

A

D

G

G

G

S

K

W

L

T

I

G

G

F

F

E

D

V

I

E

D

L

L

G

G

Q

N

A

D

L

L

C

C

E

K

K

R

M

M

Q

N

A

I

E

E

C

C

E

K

I

F

T

V

P

E

T

Q

G

A

W
|
W

S

D

G

G

I

I

F

I

A

P

A

S

L

L

D

T

S

A

G

G

K

R

I

Y

D

D

M

A

I

I

M

M

N

A

S
x
A

L

M

S
x
G

I

I

T

Q

E

P

Q

A

R
|
R

K

E

E

K

V

V

I

I

D

A

F

F

T

S

D

R

P

P

Y

Y

Y

L

F

L

T

T

P

P

S
x
M

A

T

Y

F

-

L

-

T

-

A

-

D

-

S

-

P

-

L

-

K

-

T

-

Q

V

V

A

A

A

I

K

E

S

N

D

L

S

P

F

L

D

D

-

N

-

I

I

T

P

P

E

E

G

Q

-

K

-

A

-

E

-

L

-

D

-

K

-

F

-

T

-

K

-

I

L

F

D

E

G

G

K

V

L

K

L

F

G

G

V

V

Q
|
Q

G

A

G

G

T
|
T

T
x
S

N
x
H

A

E

T

A

Y

F

A

M

R

K

R

Q

E

M

L

M

R

-

D

-

T

P

G

S

V

V

E

Q

I

I

K

S

I

T

Y

Y

D

D

Q

T

Q

I

E

D

Q

N

A

V

N

V

R

M

D

D

L

L

F

K

A

A

G

G

R

R

V

I

D

D

A

A

I

S

L

L

A

A

D
x
S

K

V

V

S

A

F

M

L

T

K

E

P

L

L

V

T

E

-

R

-

D

D

E

K

A

P

S

D

E

N

Y

K

E

D

I

L

R

K

A

M

T

F

A

G

P

P

R

R

H

M

A

T

A

G

-

G

-

L

F

F

G

G

E

K

G

G

V

V

G

G

I

V

G

G

L

I

R

R

Q

K

S

E

D

D

D

A

D

D

L

L

R

K

Q

A

R

L

L

F

N

D

Q

K

A

A

I

I

S

D

E

A

A

A

L

I

E

A

D

D

G

G

T

T

C

V

T

Q

D

K

L

L

S

S

Q

Q

E

Q

Y

W

F

F

G

G

T

Y

D

D

binding (2~{S})-5-azanyl-2-[[(2~{R})-1-oxidanyl-1-oxidanylidene-propan-2-yl]amino]pentanoic acid: Y12 (= Y35), W50 (= W73), A68 (≠ S91), G70 (≠ S93), R75 (= R98), M90 (≠ S113), Q138 (= Q137), T141 (= T140), S142 (≠ T141), H143 (≠ N142), S180 (≠ D181)

Sites not aligning to the query:

binding (2~{S})-5-azanyl-2-[[(2~{R})-1-oxidanyl-1-oxidanylidene-propan-2-yl]amino]pentanoic acid: 9

5ot9A Structure of the periplasmic binding protein (pbp) noct from a.Tumefaciens c58 in complex with histopine. (see paper)
33% identity, 85% coverage: 35:252/256 of query aligns to 12:251/254 of 5ot9A

query
sites
5ot9A

Y
|
Y

P

A

P

P

F

Y

T

N

F

F

K

K

G

D

S

A

D

G

G

G

S

K

W

L

T

I

G

G

F

F

E

D

V

I

E

D

L

L

G

G

Q

N

A

D

L

L

C

C

E

K

K

R

M

M

Q

N

A

I

E

E

C

C

E

K

I

F

T

V

P

E

T

Q

G

A

W
|
W

S

D

G

G

I

I

F

I

A

P

A

S

L

L

D

T

S

A

G

G

K

R

I

Y

D

D

M

A

I

I

M

M

N
x
A

S
x
A

L

M

S

G

I

I

T

Q

E

P

Q

A

R
|
R

K

E

E

K

V

V

I

I

D

A

F

F

T

S

D

R

P

P

Y

Y

Y

L

F

L

T

T

P

P

S
x
M

A

T

Y

F

-

L

-

T

-

A

-

D

-

S

-

P

-

L

-

K

-

T

-

Q

V

V

A

A

A

I

K

E

S

N

D

L

S

P

F

L

D

D

-

N

-

I

I

T

P

P

E

E

G

Q

-

K

-

A

-

E

-

L

-

D

-

K

-

F

-

T

-

K

-

I

L

F

D

E

G

G

K

V

L

K

L

F

G

G

V

V

Q
|
Q

G

A

G

G

T
|
T

T
x
S

N
x
H

A

E

T

A

Y

F

A

M

R

K

R

Q

E

M

L

M

R

-

D

-

T

P

G

S

V

V

E

Q

I

I

K

S

I

T

Y

Y

D

D

Q

T

Q

I

E

D

Q

N

A

V

N

V

R

M

D

D

L

L

F

K

A

A

G

G

R

R

V

I

D

D

A

A

I

S

L

L

A

A

D
x
S

K

V

V

S

A

F

M

L

T

K

E

P

L

L

V

T

E

-

R

-

D

D

E

K

A

P

S

D

E

N

Y

K

E

D

I

L

R

K

A

M

T

F

A

G

P

P

R

R

H

M

A

T

A

G

-

G

-

L

F

F

G

G

E

K

G

G

V

V

G

G

I

V

G

G

L

I

R

R

Q

K

S

E

D

D

D

A

D

D

L

L

R

K

Q

A

R

L

L

F

N

D

Q

K

A

A

I

I

S

D

E

A

A

A

L

I

E

A

D

D

G

G

T

T

C

V

T

Q

D

K

L

L

S

S

Q

Q

E

Q

Y

W

F

F

G

G

T

Y

D

D

binding Histopine: Y12 (= Y35), W50 (= W73), A67 (≠ N90), A68 (≠ S91), R75 (= R98), M90 (≠ S113), Q138 (= Q137), T141 (= T140), S142 (≠ T141), H143 (≠ N142), S180 (≠ D181)

Sites not aligning to the query:

binding Histopine: 9

4powA Structure of the pbp noct in complex with pyronopaline (see paper)
33% identity, 85% coverage: 35:252/256 of query aligns to 12:251/254 of 4powA

query
sites
4powA

Y
|
Y

P

A

P

P

F

Y

T

N

F

F

K

K

G

D

S

A

D

G

G

G

S

K

W

L

T

I

G

G

F

F

E

D

V

I

E

D

L

L

G

G

Q

N

A

D

L

L

C

C

E

K

K

R

M

M

Q

N

A

I

E

E

C

C

E

K

I

F

T

V

P

E

T

Q

G

A

W
|
W

S

D

G

G

I

I

F

I

A

P

A

S

L

L

D

T

S

A

G

G

K

R

I

Y

D

D

M

A

I

I

M

M

N
x
A

S
x
A

L

M

S
x
G

I

I

T

Q

E

P

Q

A

R
|
R

K

E

E

K

V

V

I

I

D

A

F

F

T

S

D

R

P

P

Y

Y

Y

L

F

L

T

T

P

P

S
x
M

A

T

Y

F

-

L

-

T

-

A

-

D

-

S

-

P

-

L

-

K

-

T

-

Q

V

V

A

A

A

I

K

E

S

N

D

L

S

P

F

L

D

D

-

N

-

I

I

T

P

P

E

E

G

Q

-

K

-

A

-

E

-

L

-

D

-

K

-

F

-

T

-

K

-

I

L

F

D

E

G

G

K

V

L

K

L

F

G

G

V

V

Q
|
Q

G

A

G

G

T
|
T

T
x
S

N
x
H

A

E

T

A

Y

F

A

M

R

K

R

Q

E

M

L

M

R

-

D

-

T

P

G

S

V

V

E

Q

I

I

K

S

I

T

Y

Y

D

D

Q

T

Q

I

E

D

Q

N

A

V

N

V

R

M

D

D

L

L

F

K

A

A

G

G

R

R

V

I

D

D

A

A

I

S

L

L

A

A

D
x
S

K

V

V

S

A

F

M

L

T

K

E

P

L

L

V

T

E

-

R

-

D

D

E

K

A

P

S

D

E

N

Y

K

E

D

I

L

R

K

A

M

T

F

A

G

P

P

R

R

H

M

A

T

A

G

-

G

-

L

F

F

G

G

E

K

G

G

V

V

G

G

I

V

G

G

L

I

R

R

Q

K

S

E

D

D

D

A

D

D

L

L

R

K

Q

A

R

L

L

F

N

D

Q

K

A

A

I

I

S

D

E

A

A

A

L

I

E

A

D

D

G

G

T

T

C

V

T

Q

D

K

L

L

S

S

Q

Q

E

Q

Y

W

F

F

G

G

T

Y

D

D

binding 1-[(1S)-4-carbamimidamido-1-carboxybutyl]-5-oxo-D-proline: Y12 (= Y35), W50 (= W73), A67 (≠ N90), A68 (≠ S91), G70 (≠ S93), R75 (= R98), M90 (≠ S113), Q138 (= Q137), T141 (= T140), S142 (≠ T141), H143 (≠ N142), S180 (≠ D181)

Sites not aligning to the query:

binding 1-[(1S)-4-carbamimidamido-1-carboxybutyl]-5-oxo-D-proline: 9

5ovzA High resolution structure of the pbp noct in complex with nopaline (see paper)
33% identity, 85% coverage: 35:252/256 of query aligns to 13:252/259 of 5ovzA

query
sites
5ovzA

Y
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Y

P

A

P

P

F

Y

T

N

F

F

K

K

G

D

S

A

D

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K

W

L

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I

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G

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F

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N

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N
x
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x
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R

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x
M

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-

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binding N-[(1S)-4-carbamimidamido-1-carboxybutyl]-D-glutamic acid: Y13 (= Y35), W51 (= W73), A68 (≠ N90), A69 (≠ S91), G71 (≠ S93), R76 (= R98), M91 (≠ S113), Q139 (= Q137), T142 (= T140), S143 (≠ T141), H144 (≠ N142), S181 (≠ D181), V213 (= V213)

Sites not aligning to the query:

binding N-[(1S)-4-carbamimidamido-1-carboxybutyl]-D-glutamic acid: 10

5orgA Structure of the periplasmic binding protein (pbp) occj from a. Tumefaciens b6 in complex with octopine. (see paper)
34% identity, 86% coverage: 35:253/256 of query aligns to 14:254/257 of 5orgA

query
sites
5orgA

Y
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Y

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A

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P

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binding octopine: Y14 (= Y35), W52 (= W73), A69 (≠ N90), A70 (≠ S91), M71 (≠ L92), S72 (= S93), R77 (= R98), N92 (≠ S113), Q140 (= Q137), T143 (= T140), T144 (= T141), N183 (≠ D181)

Sites not aligning to the query:

binding octopine: 11

8hqrA Crystal structure of the arginine-/lysine-binding protein sar11_1210 from 'candidatus pelagibacter ubique' htcc1062 bound to arginine (see paper)
33% identity, 89% coverage: 24:252/256 of query aligns to 8:264/267 of 8hqrA

query
sites
8hqrA

D

D

P

K

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K

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I

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P

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Y
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Y

P

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F

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F

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A

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W
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W

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G

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A

A

L

L

D

L

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I

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S

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Y

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E

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A

A

A

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-

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