SitesBLAST

Comparing WP_052591916.1 NCBI__GCF_001190945.1:WP_052591916.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 20 (the maximum) hits to proteins with known functional sites

7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
33% identity, 52% coverage: 25:235/404 of query aligns to 45:255/467 of 7qh2C

• binding flavin-adenine dinucleotide: V73 (= V53), G75 (= G55), S76 (≠ A56), G77 (= G57), T78 (= T58), G79 (≠ K59), L80 (≠ S60), A83 (≠ G63), C84 (≠ N64), P137 (≠ E117), G138 (≠ V118), E139 (≠ V119), A142 (≠ T122), T143 (= T123), G146 (= G126), N147 (≠ L127), S149 (≠ A129), T150 (≠ S130), A152 (≠ P132), G153 (≠ S133), E203 (≠ F183), G204 (= G184), I209 (= I189)

Sites not aligning to the query:

• binding flavin-adenine dinucleotide: 422, 423
• binding fe (iii) ion: 377, 384, 422

P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
35% identity, 43% coverage: 25:198/404 of query aligns to 40:213/459 of P9WIT1

Sites not aligning to the query:

• 354 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
34% identity, 46% coverage: 28:213/404 of query aligns to 44:230/465 of 3pm9A

• active site: A149 (≠ P132), L159 (≠ T142)
• binding flavin-adenine dinucleotide: P69 (≠ V53), Q70 (≠ R54), G71 (= G55), G72 (≠ A56), N73 (≠ G57), T74 (= T58), G75 (≠ K59), L76 (≠ S60), G79 (= G63), Q80 (≠ N64), L91 (= L75), L133 (vs. gap), G134 (vs. gap), A135 (vs. gap), C139 (≠ T122), T140 (= T123), G142 (= G125), G143 (= G126), S146 (≠ A129), T147 (≠ S130), A149 (≠ P132), G150 (≠ S133), E200 (≠ F183), G201 (= G184), I205 (≠ V188), I206 (= I189)

Sites not aligning to the query:

• binding flavin-adenine dinucleotide: 423

6gouA Development of alkyl glycerone phosphate synthase inhibitors: complex with inhibitor 2i (see paper)
26% identity, 61% coverage: 24:271/404 of query aligns to 125:392/554 of 6gouA

• binding (3~{S})-3-[2,6-bis(fluoranyl)phenyl]-~{N}-[(2-oxidanylidene-1,3-dihydrobenzimidazol-5-yl)methyl]butanamide: D223 (≠ E117)
• binding flavin-adenine dinucleotide: P154 (≠ V53), I155 (≠ R54), G156 (= G55), G157 (≠ A56), G158 (= G57), T159 (= T58), S160 (≠ K59), V161 (≠ S60), G164 (= G63), P222 (vs. gap), D223 (≠ E117), S224 (≠ V118), S228 (≠ T122), T229 (= T123), G232 (= G126), W233 (≠ L127), S235 (≠ A129), T236 (≠ S130), A238 (≠ P132), S239 (= S133), E288 (≠ F183), V293 (= V188), I294 (= I189)

Sites not aligning to the query:

• binding (3~{S})-3-[2,6-bis(fluoranyl)phenyl]-~{N}-[(2-oxidanylidene-1,3-dihydrobenzimidazol-5-yl)methyl]butanamide: 411, 421, 422, 423, 474, 476, 478
• binding flavin-adenine dinucleotide: 16, 512

5ae1B Ether lipid-generating enzyme agps in complex with inhibitor zinc69435460 (see paper)
26% identity, 61% coverage: 24:271/404 of query aligns to 111:377/539 of 5ae1B

• binding flavin-adenine dinucleotide: P140 (≠ V53), I141 (≠ R54), G142 (= G55), G143 (≠ A56), G144 (= G57), T145 (= T58), S146 (≠ K59), G150 (= G63), L151 (vs. gap), P208 (vs. gap), D209 (≠ E117), S210 (≠ V118), S214 (≠ T122), T215 (= T123), G218 (= G126), W219 (≠ L127), S221 (≠ A129), T222 (≠ S130), A224 (≠ P132), S225 (= S133), E274 (≠ F183), V279 (= V188), I280 (= I189)

Sites not aligning to the query:

• binding (3-(2-fluorophenyl)-n-(1-(2-oxo-2,3-dihydro-1h-benzo[d]imidazol-5-yl)ethyl)butanamide): 392, 396, 406, 407, 408, 459, 497
• binding flavin-adenine dinucleotide: 16, 497, 535

5ae3A Ether lipid-generating enzyme agps in complex with antimycin a (see paper)
26% identity, 61% coverage: 24:271/404 of query aligns to 111:378/539 of 5ae3A

• binding [(2R,3S,6S,7R,8R)-3-[(3-formamido-2-oxidanyl-phenyl)carbonylamino]-8-hexyl-2,6-dimethyl-4,9-bis(oxidanylidene)-1,5-dioxonan-7-yl] 3-methylbutanoate: R325 (≠ E234), M327 (vs. gap), Q331 (vs. gap), F334 (vs. gap), T349 (≠ S246)
• binding flavin-adenine dinucleotide: P140 (≠ V53), I141 (≠ R54), G142 (= G55), G143 (≠ A56), G144 (= G57), T145 (= T58), S146 (≠ K59), V147 (≠ S60), L151 (vs. gap), P208 (vs. gap), D209 (≠ E117), S210 (≠ V118), S214 (≠ T122), T215 (= T123), G218 (= G126), W219 (≠ L127), S221 (≠ A129), T222 (≠ S130), A224 (≠ P132), S225 (= S133), E274 (≠ F183), V279 (= V188), I280 (= I189)

Sites not aligning to the query:

• binding [(2R,3S,6S,7R,8R)-3-[(3-formamido-2-oxidanyl-phenyl)carbonylamino]-8-hexyl-2,6-dimethyl-4,9-bis(oxidanylidene)-1,5-dioxonan-7-yl] 3-methylbutanoate: 45, 49, 53, 386, 393, 396, 400, 447, 461
• binding flavin-adenine dinucleotide: 497

5ae2B Ether lipid-generating enzyme agps in complex with inhibitor 1e (see paper)
26% identity, 61% coverage: 24:271/404 of query aligns to 111:380/542 of 5ae2B

• binding flavin-adenine dinucleotide: P140 (≠ V53), G142 (= G55), G143 (≠ A56), G144 (= G57), T145 (= T58), S146 (≠ K59), G150 (= G63), L151 (vs. gap), P208 (vs. gap), D209 (≠ E117), S210 (≠ V118), S214 (≠ T122), T215 (= T123), G218 (= G126), W219 (≠ L127), S221 (≠ A129), T222 (≠ S130), A224 (≠ P132), S225 (= S133), E274 (≠ F183), G275 (= G184), V279 (= V188), I280 (= I189)

Sites not aligning to the query:

• binding flavin-adenine dinucleotide: 16, 500
• binding 3-(2-fluorophenyl)-2-methyl-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)acrylamide: 399, 409, 410, 411, 462, 464, 466, 500, 501

4bc7B Mammalian alkyldihydroxyacetonephosphate synthase: arg419his mutant (see paper)
26% identity, 61% coverage: 24:271/404 of query aligns to 111:381/543 of 4bc7B

• binding flavin-adenine dinucleotide: P140 (≠ V53), G142 (= G55), G143 (≠ A56), G144 (= G57), T145 (= T58), S146 (≠ K59), V147 (≠ S60), G150 (= G63), L151 (vs. gap), P208 (vs. gap), D209 (≠ E117), S210 (≠ V118), S214 (≠ T122), T215 (= T123), G218 (= G126), S221 (≠ A129), T222 (≠ S130), A224 (≠ P132), S225 (= S133), E274 (≠ F183), G275 (= G184), I280 (= I189)

Sites not aligning to the query:

• binding 1-dodecanol: 410, 412
• binding flavin-adenine dinucleotide: 16, 501

5adzC Ether lipid-generating enzyme agps in complex with inhibitor 1a (see paper)
26% identity, 61% coverage: 24:271/404 of query aligns to 125:395/557 of 5adzC

• binding flavin-adenine dinucleotide: P154 (≠ V53), I155 (≠ R54), G156 (= G55), G157 (≠ A56), G158 (= G57), T159 (= T58), S160 (≠ K59), P222 (vs. gap), D223 (≠ E117), S224 (≠ V118), S228 (≠ T122), T229 (= T123), G232 (= G126), W233 (≠ L127), S235 (≠ A129), T236 (≠ S130), A238 (≠ P132), S239 (= S133), E288 (≠ F183), G289 (= G184), I294 (= I189)
• binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): D223 (≠ E117)

Sites not aligning to the query:

• binding flavin-adenine dinucleotide: 16, 515
• binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): 410, 414, 424, 425, 426, 477, 515, 516

4bc9B Mammalian alkyldihydroxyacetonephosphate synthase: wild-type, adduct with cyanoethyl (see paper)
26% identity, 61% coverage: 24:270/404 of query aligns to 111:380/542 of 4bc9B

• binding propanenitrile: D209 (≠ E117)
• binding flavin-adenine dinucleotide: P140 (≠ V53), G142 (= G55), G143 (≠ A56), G144 (= G57), T145 (= T58), S146 (≠ K59), V147 (≠ S60), G150 (= G63), L151 (vs. gap), P208 (vs. gap), D209 (≠ E117), S210 (≠ V118), S214 (≠ T122), T215 (= T123), G218 (= G126), W219 (≠ L127), S221 (≠ A129), T222 (≠ S130), A224 (≠ P132), S225 (= S133), E274 (≠ F183), G275 (= G184), I280 (= I189)

Sites not aligning to the query:

• binding propanenitrile: 462, 501
• binding flavin-adenine dinucleotide: 500

4bc9A Mammalian alkyldihydroxyacetonephosphate synthase: wild-type, adduct with cyanoethyl (see paper)
25% identity, 61% coverage: 24:271/404 of query aligns to 116:393/555 of 4bc9A

• binding propanenitrile: D214 (≠ E117)
• binding flavin-adenine dinucleotide: P145 (≠ V53), I146 (≠ R54), G147 (= G55), G148 (≠ A56), G149 (= G57), T150 (= T58), S151 (≠ K59), V152 (≠ S60), G155 (= G63), L156 (vs. gap), P213 (vs. gap), D214 (≠ E117), S215 (≠ V118), S219 (≠ T122), T220 (= T123), G223 (= G126), W224 (≠ L127), S226 (≠ A129), T227 (≠ S130), A229 (≠ P132), S230 (= S133), E279 (≠ F183), V284 (= V188), I285 (= I189)

Sites not aligning to the query:

• binding propanenitrile: 475, 513, 514
• binding flavin-adenine dinucleotide: 513

P97275 Alkyldihydroxyacetonephosphate synthase, peroxisomal; Alkyl-DHAP synthase; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Cavia porcellus (Guinea pig) (see 4 papers)
27% identity, 52% coverage: 24:235/404 of query aligns to 205:420/658 of P97275

• 234:240 (vs. 53:59, 43% identical) binding FAD
• H300 (≠ V115) mutation to A: Loss of activity.
• 303:309 (vs. 117:123, 14% identical) binding FAD
• T309 (= T123) mutation to I: Impaired FAD binding and protein stability. Loss of activity.
• TRAS 316:319 (≠ SNPS 130:133) binding FAD
• S367 (= S182) mutation to A: Strongly reduced activity.
• 368:374 (vs. 183:189, 71% identical) binding FAD
• R419 (≠ E234) Important for enzyme activity; mutation to H: Loss of activity.; mutation to K: Strongly reduced activity.

Sites not aligning to the query:

• 1:58 modified: transit peptide, Peroxisome
• 469 L→P: Impaired FAD binding and protein stability. Loss of activity.
• 515 binding substrate; R→L: Impaired FAD binding and protein stability. Loss of activity.
• 576 C→A: No effect on activity.
• 578 active site, Proton donor/acceptor; Y→F: Loss of activity.
• 615 H→A: Loss of activity.
• 615:617 Important for enzyme activity
• 616 H→A: Loss of activity.
• 617 H→A: Loss of activity.
• 654:658 Important for enzyme activity

5ae2A Ether lipid-generating enzyme agps in complex with inhibitor 1e (see paper)
27% identity, 52% coverage: 24:235/404 of query aligns to 116:331/561 of 5ae2A

• binding flavin-adenine dinucleotide: P145 (≠ V53), I146 (≠ R54), G147 (= G55), G148 (≠ A56), G149 (= G57), T150 (= T58), S151 (≠ K59), G155 (= G63), L156 (vs. gap), P213 (vs. gap), D214 (≠ E117), S215 (≠ V118), S219 (≠ T122), T220 (= T123), G223 (= G126), W224 (≠ L127), S226 (≠ A129), T227 (≠ S130), A229 (≠ P132), S230 (= S133), E279 (≠ F183), V284 (= V188), I285 (= I189)

Sites not aligning to the query:

• binding flavin-adenine dinucleotide: 16, 519
• binding 3-(2-fluorophenyl)-2-methyl-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)acrylamide: 415, 418, 428, 429, 430, 481, 483, 519

5adzA Ether lipid-generating enzyme agps in complex with inhibitor 1a (see paper)
27% identity, 52% coverage: 24:235/404 of query aligns to 113:328/566 of 5adzA

• binding flavin-adenine dinucleotide: P142 (≠ V53), I143 (≠ R54), G144 (= G55), G145 (≠ A56), G146 (= G57), T147 (= T58), S148 (≠ K59), G152 (= G63), P210 (vs. gap), D211 (≠ E117), S212 (≠ V118), S216 (≠ T122), T217 (= T123), G220 (= G126), W221 (≠ L127), S223 (≠ A129), T224 (≠ S130), A226 (≠ P132), S227 (= S133), E276 (≠ F183), G277 (= G184), V281 (= V188), I282 (= I189)
• binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): D211 (≠ E117)

Sites not aligning to the query:

• binding flavin-adenine dinucleotide: 524
• binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): 423, 433, 434, 435, 486, 488, 489, 524, 525

O00116 Alkyldihydroxyacetonephosphate synthase, peroxisomal; Alkyl-DHAP synthase; Aging-associated gene 5 protein; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Homo sapiens (Human) (see 2 papers)
26% identity, 52% coverage: 24:235/404 of query aligns to 205:420/658 of O00116

• R419 (≠ E234) Important for enzyme activity; to H: in RCDP3; loss of enzyme activity; dbSNP:rs121434411

Sites not aligning to the query:

• 182 R → Q: in RCDP3; severely reduced protein levels
• 471 E → K: in RCDP3; severely reduced protein levels
• 568 T → M: in RCDP3; does not affect protein levels; dbSNP:rs387907214

5ae1A Ether lipid-generating enzyme agps in complex with inhibitor zinc69435460 (see paper)
27% identity, 52% coverage: 24:235/404 of query aligns to 110:325/560 of 5ae1A

• binding flavin-adenine dinucleotide: P139 (≠ V53), I140 (≠ R54), G141 (= G55), G142 (≠ A56), G143 (= G57), T144 (= T58), S145 (≠ K59), V146 (≠ S60), G149 (= G63), L150 (vs. gap), P207 (vs. gap), D208 (≠ E117), S209 (≠ V118), S213 (≠ T122), T214 (= T123), G216 (= G125), G217 (= G126), W218 (≠ L127), S220 (≠ A129), T221 (≠ S130), A223 (≠ P132), S224 (= S133), E273 (≠ F183), G274 (= G184), V278 (= V188), I279 (= I189)

Sites not aligning to the query:

• binding (3-(2-fluorophenyl)-n-(1-(2-oxo-2,3-dihydro-1h-benzo[d]imidazol-5-yl)ethyl)butanamide): 416, 417, 427, 428, 429, 480, 518
• binding flavin-adenine dinucleotide: 518

8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
33% identity, 52% coverage: 25:235/404 of query aligns to 40:247/455 of 8jdpA