SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_052664679.1 NCBI__GCF_000969705.1:WP_052664679.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WKJ7 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
61% identity, 99% coverage: 1:332/336 of query aligns to 1:332/337 of P9WKJ7

query
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P9WKJ7

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M1 (= M1) modified: Initiator methionine, Removed
D192 (= D192) binding Mg(2+); binding Mg(2+)
E196 (= E196) binding Mg(2+)
E228 (= E228) binding Mg(2+)
E232 (= E232) binding Mg(2+)

4ypoA Crystal structure of mycobacterium tuberculosis ketol-acid reductoisomerase in complex with mg2+ (see paper)
61% identity, 97% coverage: 5:329/336 of query aligns to 1:325/325 of 4ypoA

query
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4ypoA

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active site: K128 (= K132), D188 (= D192), E192 (= E196)
binding magnesium ion: D188 (= D192), E192 (= E196), E224 (= E228), E228 (= E232)

7rduA Crystal structure of campylobacter jejuni keto said reductoisomerase in complex with magnesium and oxidixized and reduced NADPH
54% identity, 98% coverage: 2:330/336 of query aligns to 1:329/329 of 7rduA

query
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7rduA

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binding nadp nicotinamide-adenine-dinucleotide phosphate: G24 (= G25), G26 (= G27), S27 (= S28), Q28 (= Q29), R48 (= R49), S51 (= S52), S53 (= S54), A81 (= A82), P82 (= P83), D83 (= D84), I89 (≠ M90), A107 (≠ G108), H108 (= H109), P130 (= P131), K131 (= K132), A132 (≠ S133)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G24 (= G25), F25 (≠ Y26), G26 (= G27), S27 (= S28), Q28 (= Q29), S51 (= S52), S53 (= S54), L80 (= L81), P82 (= P83), D83 (= D84), I89 (≠ M90), A107 (≠ G108), H108 (= H109)

8upqB Campylobacter jejuni ketol-acid reductoisomerase in complex with 2,3- dihydroxy-3-isovalerate.
54% identity, 98% coverage: 2:330/336 of query aligns to 1:329/342 of 8upqB

query
sites
8upqB

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I

S

A

W

D

M

M

R

R

Y

Y

S

S

V

I

S
|
S

E

N

T

T

A
|
A

E

E

Y

Y

G

G

D

D

Y

Y

V

I

S

T

G

G

P

P

R

K

V

I

I

I

D

T

D

E

R

E

A

T

K

K

Q

K

A

A

M

M

R

K

D

G

I

V

L

L

T

K

D

D

I

I

Q

Q

D

N

G

G

T

V

F

F

A

A

A

K

A

D

L

F

A

I

D

L

E

E

W

R

E

R

G

A

G

G

A

F

K

A

T

R

F

M

E

H

A

A

N

E

R

R

A

K

A

N

G

M

K

N

D

D

H

S

Q

L

I

I

E

E

Q

K

V

T

G

G

R

R

K

N

V

L

R

R

D

A

M

M

I

M

P

P

F

W

I

I

T

S

binding magnesium ion: D191 (= D192), E195 (= E196)
binding (2R)-2,3-dihydroxy-3-methylbutanoic acid: A132 (≠ S133), P133 (= P134), D191 (= D192), E195 (= E196), E231 (= E232), S252 (= S253), A255 (= A256)

8uppA Campylobacter jejuni ketol-acid reductoisomerase in complex with NADPH and hoe704
54% identity, 97% coverage: 4:329/336 of query aligns to 2:327/327 of 8uppA

query
sites
8uppA

T

T

I

V

F

Y

Y

Y

D

D

Q

K

D

D

A

C

D

D

L

L

S

N

L

L

I

I

Q

K

G

S

R

K

T

K

V

V

A

A

V

I

L

I

G

G

Y
x
F

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

R

R

D

D

S

N

G

G

V

V

D

N

V

V

R

T

V

I

G

G

L

L

R
|
R

P

E

G

G

S
|
S

A

V

S

S

R

A

A

V

K

K

A

A

E

K

E

N

A

A

G

G

L

F

K

E

V

V

L

M

D

S

T

V

N

S

A

E

A

A

A

S

A

K

E

I

A

A

D

D

V

V

V

I

V

M

I

I

L
|
L

A

A

P
|
P

D
|
D

T

E

V
x
I

Q
|
Q

K

A

Q

D

M
x
I

W

F

H

N

D

V

G

E

L

I

A

K

D

P

Q

N

L

L

T

S

E

E

G

G

D

K

A

A

L

I

V

A

F

F

G

A

H
|
H

G

G

F

F

N

N

V

I

H

H

Y

Y

G

G

W

Q

I

I

D

V

P

V

P

P

A

K

G

G

I

V

D

D

V

V

F

I

L

M

V

I

A

A

P

P

K

K

S
x
A

P
|
P

G

G

H

H

I

T

A

V

R

R

R

N

T

E

Y

F

E

T

Q

L

G

G

Q

G

G

G

V

T

P

P

G

C

L

L

V

I

A

A

V

I

H

H

Q

Q

D

D

A

E

T

S

G

K

T

N

A

A

L

K

Q

N

L

L

G

A

V

L

S

S

Y

Y

A

A

A

S

A

A

L

I

G

G

L

G

T

G

R

R

A

T

G

G

V

I

L

I

Q

E

T

T

F

F

A

K

E

A

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

V

L

S

S

H

A

L

L

V

I

Q

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

Q

E

P

P

E

E

A

M

A

A

Y

Y

F

F

E

E

T

C

L

L

H

H

E
|
E

L

M

K

K

L

L

I

I

V

V

D

D

M

L

I

I

F

Y

E

Q

G

G

L

I

S

A

W

D

M

M

R

R

Y

Y

S

S

V
x
I

S
|
S

E

N

T

T

A
|
A

E

E

Y

Y

G

G

D

D

Y

Y

V

I

S

T

G

G

P

P

R

K

V

I

I

I

D

T

D

E

R

E

A

T

K

K

Q

K

A

A

M

M

R

K

D

G

I

V

L

L

T

K

D

D

I

I

Q

Q

D

N

G

G

T

V

F

F

A

A

A

K

A

D

L

F

A

I

D

L

E

E

W

R

E

R

G

A

G

G

A

F

K

A

T

R

F

M

E

H

A

A

N

E

R

R

A

K

A

N

G

M

K

N

D

D

H

S

Q

L

I

I

E

E

Q

K

V

T

G

G

R

R

K

N

V

L

R

R

D

A

M

M

I

M

P

P

F

W

I

I

binding magnesium ion: D190 (= D192), E194 (= E196)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F24 (≠ Y26), G25 (= G27), S26 (= S28), Q27 (= Q29), R47 (= R49), S50 (= S52), L79 (= L81), P81 (= P83), D82 (= D84), I84 (≠ V86), Q85 (= Q87), I88 (≠ M90), H107 (= H109), P132 (= P134), I250 (≠ V252), S251 (= S253)
binding (2R)-(dimethylphosphoryl)(hydroxy)acetic acid: A131 (≠ S133), P132 (= P134), D190 (= D192), E194 (= E196), E230 (= E232), I250 (≠ V252), S251 (= S253), A254 (= A256)

8sxdA Campylobacter jejuni keto-acid reductoisomerase in complex with intermediate and NADP+
54% identity, 97% coverage: 4:329/336 of query aligns to 2:327/327 of 8sxdA

query
sites
8sxdA

T

T

I

V

F

Y

Y

Y

D

D

Q

K

D

D

A

C

D

D

L

L

S

N

L

L

I

I

Q

K

G

S

R

K

T

K

V

V

A

A

V

I

L

I

G
|
G

Y
x
F

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

R

R

D

D

S

N

G

G

V

V

D

N

V

V

R

T

V

I

G

G

L

L

R

R

P

E

G

G

S
|
S

A

V

S
|
S

R

A

A

V

K

K

A

A

E

K

E

N

A

A

G

G

L

F

K

E

V

V

L

M

D

S

T

V

N

S

A

E

A

A

A

S

A

K

E

I

A

A

D

D

V

V

V

I

V

M

I

I

L
|
L

A
|
A

P
|
P

D
|
D

T

E

V

I

Q

Q

K

A

Q

D

M

I

W

F

H

N

D

V

G

E

L

I

A

K

D

P

Q

N

L

L

T

S

E

E

G

G

D

K

A

A

L

I

V

A

F

F

G

A

H

H

G

G

F

F

N

N

V

I

H

H

Y

Y

G

G

W

Q

I

I

D

V

P

V

P

P

A

K

G

G

I

V

D

D

V

V

F

I

L

M

V

I

A

A

P
|
P

K

K

S

A

P

P

G

G

H

H

I

T

A

V

R

R

R

N

T

E

Y

F

E

T

Q

L

G

G

Q

G

G

G

V

T

P

P

G

C

L

L

V

I

A

A

V

I

H

H

Q

Q

D

D

A

E

T

S

G

K

T

N

A

A

L

K

Q

N

L

L

G

A

V

L

S

S

Y

Y

A

A

A

S

A

A

L

I

G

G

L

G

T

G

R

R

A

T

G

G

V

I

L

I

Q

E

T

T

F

F

A

K

E

A

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

V

L

S

S

H

A

L

L

V

I

Q

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

Q

E

P

P

E

E

A

M

A

A

Y

Y

F

F

E

E

T

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

M

L

I

I

F

Y

E

Q

G

G

L

I

S

A

W

D

M

M

R

R

Y

Y

S

S

V

I

S

S

E

N

T

T

A

A

E

E

Y

Y

G

G

D

D

Y

Y

V

I

S

T

G

G

P

P

R

K

V

I

I

I

D

T

D

E

R

E

A

T

K

K

Q

K

A

A

M

M

R

K

D

G

I

V

L

L

T

K

D

D

I

I

Q

Q

D

N

G

G

T

V

F

F

A

A

A

K

A

D

L

F

A

I

D

L

E

E

W

R

E

R

G

A

G

G

A

F

K

A

T

R

F

M

E

H

A

A

N

E

R

R

A

K

A

N

G

M

K

N

D

D

H

S

Q

L

I

I

E

E

Q

K

V

T

G

G

R

R

K

N

V

L

R

R

D

A

M

M

I

M

P

P

F

W

I

I

binding magnesium ion: D190 (= D192), E194 (= E196)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G23 (= G25), F24 (≠ Y26), G25 (= G27), S26 (= S28), Q27 (= Q29), S50 (= S52), S52 (= S54), L79 (= L81), A80 (= A82), P81 (= P83), D82 (= D84), P129 (= P131)
binding 3-hydroxy-3-methyl-2-oxobutanoic acid: D190 (= D192), E194 (= E196)

D0WGK0 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Slackia exigua (strain ATCC 700122 / DSM 15923 / CIP 105133 / JCM 11022 / KCTC 5966 / S-7) (see paper)
55% identity, 98% coverage: 1:329/336 of query aligns to 10:338/342 of D0WGK0

query
sites
D0WGK0

M

M

A

A

A

V

T

T

I

I

F

L

Y

Y

D

E

Q

Q

D

D

A

V

D

D

L

P

S

K

L

V

I

I

Q

Q

G

G

R

L

T

K

V

V

A

G

V

I

L

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

L

N

N

L

L

R

M

D

D

S

S

G

G

V

V

D

D

V

V

R

R

V

V

G

G

L

L

R
|
R

P

E

G

G

S
|
S

A

S

S
|
S

R

W

A

K

K

T

A

A

E

E

A

A

G

G

L

L

K

K

V

V

L

T

D

D

T

M

N

D

A

T

A

A

A

E

E

E

A

A

D

D

V

V

V

I

V

M

I

V

L

L

A

V

P

P

D
|
D

T
x
E

V
x
I

Q
|
Q

K

P

Q

K

M

V

W

Y

H

Q

D

E

G

H

L

I

A

A

D

A

Q

H

L

L

T

K

E

A

G

G

D

N

A

T

L

L

V

A

F

F

G

A

H

H

G

G

F

F

N

N

V

I

H

H

Y

Y

G

G

W

Y

I

I

D

V

P

P

A

E

G

D

I

V

D

N

V

V

F

I

L

M

V

C

A

A

P

P

K

K

S

G

P

P

G
|
G

H

H

I

I

A

V

R

R

T

Q

Y

F

E

T

Q

E

G

G

Q

S

G

G

V

V

P

P

G

D

L

L

V

A

A

C

V

V

H

Q

Q

Q

D

D

A

A

T

T

G

G

T

N

A

A

L

W

Q

D

L

I

G

V

V

L

S

S

Y

Y

A

C

A

W

A

G

L

V

G

G

L

G

T

A

R

R

A

S

G

G

V

I

L

I

Q

K

T

A

T

T

F

F

A

A

E

E

T

T

E

E

T

E

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

V

L

S

V

H

E

L

L

V

V

Q

K

A

A

G

G

F

F

E

E

T

T

L

L

V

T

E

E

A

A

G

G

Y

Y

Q

P

P

P

E

E

A

L

A

A

Y

Y

F

F

E

E

T

C

L

Y

H

H

E

E

L

M

K

K

L

M

I

I

V

V

D

D

M

L

I

M

F

Y

E

E

G

S

G

G

L

I

S

H

W

F

M

M

R

N

Y

Y

S

S

V

I

S
|
S

E

N

T

T

A

A

E

E

Y

Y

G

G

D

E

Y

Y

V

Y

S

A

G

G

P

P

R

K

V

V

I

I

D

N

D

E

R

Q

A

S

K

R

Q

E

A

A

M

M

R

K

D

E

I

I

L

L

T

K

D

R

I

I

Q

Q

D

D

G

G

T

S

F

F

A

A

A

Q

A

E

L

F

A

V

D

D

E

D

W

C

E

N

G

N

G

G

A

H

K

K

T

R

F

L

E

L

A

E

N

Q

R

R

A

E

A

A

G

I

K

N

D

T

H

H

Q

P

I

I

E

E

Q

T

V

T

G

G

R

A

K

Q

V

I

R

R

D

S

M

M

I

F

P

S

F

W

I

I

YGSQ 35:38 (= YGSQ 26:29) binding NADP(+)
R58 (= R49) binding NADP(+)
S61 (= S52) binding NADP(+); mutation to D: Inversion of cofactor specificity from NADPH to NADH. Strong decrease of the affinity for NADPH and 2.5-fold decrease of the affinity for NADH. 8-fold decrease of the catalytic efficiency for NADPH and 2-fold increase of the catalytic efficiency for NADH; when associated with D-63.
S63 (= S54) binding NADP(+); mutation to D: Inversion of cofactor specificity from NADPH to NADH. Strong decrease of the affinity for NADPH and 2.5-fold decrease of the affinity for NADH. 8-fold decrease of the catalytic efficiency for NADPH and 2-fold increase of the catalytic efficiency for NADH; when associated with D-61.
DEIQ 93:96 (≠ DTVQ 84:87) binding NADP(+)
G144 (= G135) binding NADP(+)
D201 (= D192) binding Mg(2+)
E205 (= E196) binding Mg(2+)
S262 (= S253) binding substrate

8swmA Crystal structure of campylobacter jejuni ketol-acid reductoisomerase in complex with 2-acetolactate
54% identity, 96% coverage: 6:328/336 of query aligns to 1:323/323 of 8swmA

query
sites
8swmA

F

Y

Y

Y

D

D

Q

K

D

D

A

C

D

D

L

L

S

N

L

L

I

I

Q

K

G

S

R

K

T

K

V

V

A

A

V

I

L

I

G

G

Y

F

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

R

R

D

D

S

N

G

G

V

V

D

N

V

V

R

T

V

I

G

G

L

L

R

R

P

E

G

G

S

S

A

V

S

S

R

A

A

V

K

K

A

A

E

K

E

N

A

A

G

G

L

F

K

E

V

V

L

M

D

S

T

V

N

S

A

E

A

A

A

S

A

K

E

I

A

A

D

D

V

V

V

I

V

M

I

I

L

L

A

A

P

P

D

D

T

E

V

I

Q

Q

K

A

Q

D

M

I

W

F

H

N

D

V

G

E

L

I

A

K

D

P

Q

N

L

L

T

S

E

E

G

G

D

K

A

A

L

I

V

A

F

F

G

A

H

H

G

G

F

F

N

N

V

I

H

H

Y

Y

G

G

W

Q

I

I

D

V

P

V

P

P

A

K

G

G

I

V

D

D

V

V

F

I

L

M

V

I

A

A

P

P

K

K

S

A

P

P

G

G

H

H

I

T

A

V

R

R

R

N

T

E

Y

F

E

T

Q

L

G

G

Q

G

G

G

V

T

P

P

G

C

L

L

V

I

A

A

V

I

H

H

Q

Q

D

D

A

E

T

S

G

K

T

N

A

A

L

K

Q

N

L

L

G

A

V

L

S

S

Y

Y

A

A

A

S

A

A

L

I

G

G

L

G

T

G

R

R

A

T

G

G

V

I

L

I

Q

E

T

T

F

F

A

K

E

A

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

V

L

S

S

H

A

L

L

V

I

Q

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

Q

E

P

P

E

E

A

M

A

A

Y

Y

F

F

E

E

T

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

M

L

I

I

F

Y

E

Q

G

G

L

I

S

A

W

D

M

M

R

R

Y

Y

S

S

V

I

S

S

E

N

T

T

A

A

E

E

Y

Y

G

G

D

D

Y

Y

V

I

S

T

G

G

P

P

R

K

V

I

I

I

D

T

D

E

R

E

A

T

K

K

Q

K

A

A

M

M

R

K

D

G

I

V

L

L

T

K

D

D

I

I

Q

Q

D

N

G

G

T

V

F

F

A

A

A

K

A

D

L

F

A

I

D

L

E

E

W

R

E

R

G

A

G

G

A

F

K

A

T

R

F

M

E

H

A

A

N

E

R

R

A

K

A

N

G

M

K

N

D

D

H

S

Q

L

I

I

E

E

Q

K

V

T

G

G

R

R

K

N

V

L

R

R

D

A

M

M

I

M

P

P

F

W

binding magnesium ion: D187 (= D192), E191 (= E196)
binding (2S)-2-hydroxy-2-methyl-3-oxobutanoic acid: D187 (= D192), E191 (= E196)

4kqxB Mutant slackia exigua kari ddv in complex with NAD and an inhibitor (see paper)
55% identity, 98% coverage: 1:329/336 of query aligns to 1:329/335 of 4kqxB

query
sites
4kqxB

M

M

A

A

A

V

T

T

I

I

F

L

Y

Y

D

E

Q

Q

D

D

A

V

D

D

L

P

S

K

L

V

I

I

Q

Q

G

G

R

L

T

K

V

V

A

G

V

I

L

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G
|
G

H

H

A

A

H

H

A

A

L

L

N

N

L

L

R

M

D

D

S

S

G

G

V

V

D

D

V

V

R

R

V

V

G

G

L

L

R
|
R

P

E

G

G

S

D

A

S

S

D

R

W

A

K

K

T

A

A

E

E

A

A

G

G

L

L

K

K

V

V

L

T

D

D

T

M

N

D

A

T

A

A

A
|
A

A
x
E

E

E

A
|
A

D

D

V

V

V

I

V

M

I

V

L
|
L

A
x
V

P
|
P

D
|
D

T

E

V

V

Q

Q

K

P

Q

K

M
x
V

W

Y

H

Q

D

E

G

H

L

I

A

A

D

A

Q

H

L

L

T

K

E

A

G

G

D
x
N

A

T

L

L

V

A

F

F

G

A

H
|
H

G

G

F

F

N

N

V

I

H

H

Y

Y

G

G

W

Y

I

I

D

V

P

P

A

E

G

D

I

V

D

N

V

V

F

I

L

M

V

C

A

A

P

P

K
|
K

S

G

P
|
P

G

G

H

H

I

I

A

V

R

R

T

Q

Y

F

E

T

Q

E

G

G

Q

S

G

G

V

V

P

P

G

D

L

L

V

A

A

C

V

V

H

Q

Q

Q

D

D

A

A

T

T

G

G

T

N

A

A

L

W

Q

D

L

I

G

V

V

L

S

S

Y

Y

A

C

A

W

A

G

L

V

G

G

L

G

T

A

R

R

A

S

G

G

V

I

L

I

Q

K

T

A

T

T

F

F

A

A

E

E

T

T

E

E

T

E

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

V

L

S

V

H

E

L

L

V

V

Q

K

A

A

G

G

F

F

E

E

T

T

L

L

V

T

E

E

A

A

G

G

Y

Y

Q

P

P

P

E

E

A

L

A

A

Y

Y

F

F

E

E

T

C

L

Y

H

H

E
|
E

L

M

K

K

L

M

I

I

V

V

D

D

M

L

I

M

F

Y

E

E

G

S

G

G

L

I

S

H

W

F

M

M

R
x
N

Y

Y

S
|
S

V
x
I

S
|
S

E
x
N

T

T

A
|
A

E
|
E

Y

Y

G

G

D

E

Y

Y

V

Y

S

A

G

G

P

P

R

K

V

V

I

I

D

N

D

E

R

Q

A

S

K

R

Q

E

A

A

M

M

R

K

D

E

I

I

L

L

T

K

D

R

I

I

Q

Q

D

D

G

G

T

S

F

F

A

A

A

Q

A

E

L

F

A

V

D

D

E

D

W

C

E

N

G

N

G

G

A

H

K

K

T

R

F

L

E

L

A

E

N

Q

R

R

A

E

A

A

G

I

K

N

D

T

H

H

Q

P

I

I

E

E

Q

T

V

T

G

G

R

A

K

Q

V

I

R

R

D

S

M

M

I

F

P

S

F

W

I

I

active site: K132 (= K132), D192 (= D192), E196 (= E196)
binding n-hydroxy-n-isopropyloxamic acid: P134 (= P134), D192 (= D192), E196 (= E196), E232 (= E232), I252 (≠ V252), S253 (= S253), A256 (= A256)
binding magnesium ion: G27 (= G27), Q29 (= Q29), G30 (= G30), A72 (= A72), E73 (≠ A73), A75 (= A75), L81 (= L81), N103 (≠ D103), D192 (= D192), E196 (= E196), N249 (≠ R249), S251 (= S251), I252 (≠ V252), I252 (≠ V252), S253 (= S253), N254 (≠ E254), E257 (= E257)
binding nicotinamide-adenine-dinucleotide: Y26 (= Y26), G27 (= G27), S28 (= S28), Q29 (= Q29), R49 (= R49), L81 (= L81), V82 (≠ A82), P83 (= P83), D84 (= D84), V90 (≠ M90), H109 (= H109), P134 (= P134), S251 (= S251), I252 (≠ V252), S253 (= S253)

Sites not aligning to the query:

binding histidine: 333, 334, 335

4kqwA The structure of the slackia exigua kari in complex with NADP (see paper)
55% identity, 97% coverage: 4:329/336 of query aligns to 1:326/326 of 4kqwA

query
sites
4kqwA

T

T

I

I

F

L

Y

Y

D

E

Q

Q

D

D

A

V

D

D

L

P

S

K

L

V

I

I

Q

Q

G

G

R

L

T

K

V

V

A

G

V

I

L

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

L

N

N

L

L

R

M

D

D

S

S

G

G

V

V

D

D

V

V

R

R

V

V

G

G

L

L

R
|
R

P

E

G

G

S
|
S

A

S

S
|
S

R

W

A

K

K

T

A

A

E

E

A

A

G

G

L

L

K

K

V

V

L

T

D

D

T

M

N

D

A

T

A

A

A

E

E

E

A

A

D

D

V

V

V

I

V

M

I

V

L
|
L

A
x
V

P
|
P

D
|
D

T

E

V
x
I

Q
|
Q

K

P

Q

K

M

V

W

Y

H

Q

D

E

G

H

L

I

A

A

D

A

Q

H

L

L

T

K

E

A

G

G

D

N

A

T

L

L

V

A

F

F

G

A

H
|
H

G

G

F

F

N

N

V

I

H

H

Y

Y

G

G

W

Y

I

I

D

V

P

P

A

E

G

D

I

V

D

N

V

V

F

I

L

M

V

C

A

A

P

P

K
|
K

S

G

P
|
P

G

G

H

H

I

I

A

V

R

R

T

Q

Y

F

E

T

Q

E

G

G

Q

S

G

G

V

V

P

P

G

D

L

L

V

A

A

C

V

V

H

Q

Q

Q

D

D

A

A

T

T

G

G

T

N

A

A

L

W

Q

D

L

I

G

V

V

L

S

S

Y

Y

A

C

A

W

A

G

L

V

G

G

L

G

T

A

R

R

A

S

G

G

V

I

L

I

Q

K

T

A

T

T

F

F

A

A

E

E

T

T

E

E

T

E

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

V

L

S

V

H

E

L

L

V

V

Q

K

A

A

G

G

F

F

E

E

T

T

L

L

V

T

E

E

A

A

G

G

Y

Y

Q

P

P

P

E

E

A

L

A

A

Y

Y

F

F

E

E

T

C

L

Y

H

H

E

E

L

M

K

K

L

M

I

I

V

V

D

D

M

L

I

M

F

Y

E

E

G

S

G

G

L

I

S

H

W

F

M

M

R

N

Y

Y

S

S

V
x
I

S
|
S

E

N

T

T

A

A

E

E

Y

Y

G

G

D

E

Y

Y

V

Y

S

A

G

G

P

P

R

K

V

V

I

I

D

N

D

E

R

Q

A

S

K

R

Q

E

A

A

M

M

R

K

D

E

I

I

L

L

T

K

D

R

I

I

Q

Q

D

D

G

G

T

S

F

F

A

A

A

Q

A

E

L

F

A

V

D

D

E

D

W

C

E

N

G

N

G

G

A

H

K

K

T

R

F

L

E

L

A

E

N

Q

R

R

A

E

A

A

G

I

K

N

D

T

H

H

Q

P

I

I

E

E

Q

T

V

T

G

G

R

A

K

Q

V

I

R

R

D

S

M

M

I

F

P

S

F

W

I

I

active site: K129 (= K132), D189 (= D192), E193 (= E196)
binding magnesium ion: D189 (= D192), E193 (= E196)
binding nadp nicotinamide-adenine-dinucleotide phosphate: Y23 (= Y26), G24 (= G27), S25 (= S28), Q26 (= Q29), R46 (= R49), S49 (= S52), S51 (= S54), L78 (= L81), V79 (≠ A82), P80 (= P83), D81 (= D84), I83 (≠ V86), Q84 (= Q87), H106 (= H109), P131 (= P134), I249 (≠ V252), S250 (= S253)

C1DFH7 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Azotobacter vinelandii (strain DJ / ATCC BAA-1303) (see paper)
56% identity, 97% coverage: 5:329/336 of query aligns to 3:327/338 of C1DFH7

query
sites
C1DFH7

I

V

F

Y

Y

Y

D

D

Q

K

D

D

A

C

D

D

L

L

S

S

L

I

I

I

Q

Q

G

S

R

K

T

K

V

V

A

A

V

I

L

I

G

G

Y

Y

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

A

L

C

N

N

L

L

R

K

D

D

S

S

G

G

V

V

D

D

V

V

R

Y

V

V

G

G

L

L

R

R

P

A

G

G

S

S

A

A

S

S

R

V

A

A

K

K

A

A

E

E

E

A

A

H

G

G

L

L

K

T

V

V

L

K

D

S

T

V

N

K

A

D

A

A

A

V

A

A

E

A

A

A

D

D

V

V

V

M

I

I

L

L

A

T

P

P

D

D

T

E

V

F

Q

Q

K

G

Q

R

M

L

W

Y

H

K

D

D

G

E

L

I

A

E

D

P

Q

N

L

L

T

K

E

K

G

G

D

A

A

T

L

L

V

A

F

F

G

A

H

H

G

G

F

F

N

S

V

I

H

H

Y

Y

G

N

W

Q

I

V

D

V

P

P

P

R

A

A

G

D

I

L

D

D

V

V

F

I

L

M

V

I

A

A

P

P

K

K

S

A

P

P

G

G

H

H

I

T

A

V

R

R

R

S

T

E

Y

F

E

V

Q

R

G

G

Q

G

G

G

V

I

P

P

G

D

L

L

V

I

A

A

V

V

H

Y

Q

Q

D

D

A

A

T

S

G

G

T

N

A

A

L

K

Q

N

L

L

G

A

V

L

S

S

Y

Y

A

A

A

C

A

G

L

V

G

G

L

G

T

G

R

R

A

T

G

G

V

I

L

I

Q

E

T

T

F

F

A

K

E

D

E

E

T

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E

E

Q

Q

A

A

V

V

L

L

C

C

G

G

V

C

S

V

H

E

L

L

V

V

Q

K

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

Q

A

P

P

E

E

A

M

A

A

Y

Y

F

F

E
|
E

T

C

L

L

H

H

E
|
E

L

L

K

K

L

L

I

I

V

V

D

D

M

L

I

M

F

F

E

E

G

G

L

I

S

A

W

N

M

M

R

N

Y

Y

S

S

V

I

S

S

E

N

T

N

A

A

E

E

Y

Y

G

G

D

E

Y

Y

V

V

S

T

G

G

P

P

R

E

V

V

I

I

D

N

D

E

R

Q

A

S

K

R

Q

Q

A

A

M

M

R

R

D

N

I

A

L

L

T

K

D

R

I

I

Q

Q

D

D

G

G

T

E

F

Y

A

A

A

K

A

M

L

F

A

I

D

T

E

E

W

G

E

A

G

A

G

N

A

Y

K

P

T

S

F

M

E

T

A

A

N

Y

R

R

A

R

A

N

G

N

K

A

D

A

H

H

Q

Q

I

I

E

E

Q

V

V

V

G

G

R

E

K

K

V

L

R

R

D

T

M

M

I

M

P

P

F

W

I

I

D190 (= D192) binding Mg(2+)
E226 (= E228) binding Mg(2+)
E230 (= E232) binding Mg(2+)

4xiyA Crystal structure of ketol-acid reductoisomerase from azotobacter (see paper)
56% identity, 97% coverage: 5:329/336 of query aligns to 3:327/328 of 4xiyA

query
sites
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active site: K130 (= K132), D190 (= D192), E194 (= E196)
binding fe (iii) ion: D190 (= D192), E194 (= E196)
binding magnesium ion: E226 (= E228), E230 (= E232)

6jx2B Crystal structure of ketol-acid reductoisomerase from corynebacterium glutamicum (see paper)
60% identity, 97% coverage: 5:329/336 of query aligns to 3:327/327 of 6jx2B

query
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6jx2B

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binding magnesium ion: A73 (= A75), N98 (≠ T100), D101 (= D103), D190 (= D192), D190 (= D192), E194 (= E196), E194 (= E196), E226 (= E228), E230 (= E232)

6jx2A Crystal structure of ketol-acid reductoisomerase from corynebacterium glutamicum (see paper)
61% identity, 96% coverage: 5:326/336 of query aligns to 3:324/325 of 6jx2A

query
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6jx2A

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