SitesBLAST

2cnbA Trypanosoma brucei udp-galactose-4-epimerase in ternary complex with NAD and the substrate analogue udp-4-deoxy-4-fluoro-alpha-d-galactose (see paper)
40% identity, 100% coverage: 1:324/325 of query aligns to 3:365/366 of 2cnbA

query
sites
2cnbA

M

M

R

R

V

V

L

L

V

V

T

C

G
|
G

G

G

A

A

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

F

T

V

A

R

A

A

L

L

-

R

G

D

A

T

G

N

H

H

E

S

V

V

T

V

V

I

L

V

D
|
D

D
x
S

L

L

-
x
V

-
x
G

S

T

T

H

G

G

H

K

R

S

D

D

A

H

V

V

P

E

-

T

-

R

-

E

-

N

A

V

G

A

A

R

E

K

L

L

V

Q

A

S

D

D

A

G

T

P

D

K

P

P

-

P

-

W

-

A

-

D

-

R

-

Y

-

A

T

L

A

E

V

V

G

G

P
x
D

V
|
V

V

R

G

N

D

E

-

D

-

F

-

L

-

N

-

G

-

V

-

F

-

T

-

R

-

H

G

G

R

P

F

I

D

D

A

A

C

V

I

V

H

H

F
x
M

A
x
C

A
|
A

L

F

I
x
L

E

A

A

V

G

G

E

E

S

S

M

V

R

R

H

D

P

P

E

L

R

K

F

Y

T

D

V
x
N

N

N

T

V

A

V

G

G

T

I

A

L

R

R

L

L

E

Q

V

A

L

M

I

L

D

L

H

H

A

K

V

C

P

D

R

K

F

I

V

I

L

F

S

S

S
|
S

T
x
S

A
|
A

V

I

Y

F

G

G

E

N

P

P

E

T

R

N

T

A

-

E

P

P

I

I

D

D

E

I

D

N

H

A

P

K

L

K

A

S

P

P

T

E

N

S

A

P

Y
|
Y

G

G

E

E

S

S

K
|
K

L

L

L

I

I

A

E

E

R

R

M

M

L

I

A

R

W

D

H

C

H

A

R

E

I

A

H

Y

G

G

L

I

R

K

T

G

A

I

A

C

L

L

R

R

Y
|
Y

F
|
F

N
|
N

A
|
A

A

C

G

G

A

A

T

H

P

E

G

D

R

G

-

D

-

I

G

G

E

E

R

H

H

Y

D

Q

P

G

E

S

T

T

H
|
H

L
|
L

I

I

P

P

L

I

V

I

L

L

-

G

-

R

-

V

-

M

Q

S

V

D

A

I

A

A

G

P

E

D

R

D

D

K

Q

R

V

M

S
x
P

I
|
I

F
|
F

G

G

S

T

D

D

Y

Y

P

P

T

T

P

P

D

D

G

G

T

T

A
x
C

I

V

R
|
R

D

D

Y

Y

V

V

H

H

V

V

A

C

D

D

L

L

A

A

D

S

A

A

H

H

V

I

R

L

A

A

V

L

E

D

R

Y

L

V

D

E

Q

K

L

L

G

G

T

P

-

N

-

D

-

K

-

S

-

K

-

Y

-

F

C

S

T

V

V

F

N

N

L

L

G

G

S

T

G

S

T

R

G

G

F

Y

S

S

V
|
V

R

R

E

E

V

V

I

I

D

E

A

V

A

A

R

R

R

K

V

T

T

T

G

G

H

H

A

P

I

I

P

P

V

V

I

R

E

E

A

C

D

G

R

R

R
|
R

A

E

G

G

D
|
D

P

P

A

A

R

Y

L

L

V

V

A

A

S

A

A

S

T

D

R

K

A

A

R

R

E

E

L

V

L

L

G

G

W

W

E

K

P

P

A

K

I

Y

T

D

D

T

L

L

D

E

R

A

I

I

V

M

A

E

D

T

A

S

W

W

N

K

A

F

T

Q

V

R

D

T

H

H

A

P

G

N

A

G

H

Y

active site: S144 (≠ T117), A145 (= A118), A146 (= A119), Y169 (= Y141), K173 (= K145)
binding nicotinamide-adenine-dinucleotide: G9 (= G7), G12 (= G10), Y13 (≠ F11), I14 (= I12), D34 (= D31), S35 (≠ D32), V37 (vs. gap), G38 (vs. gap), D77 (≠ P60), V78 (= V61), M100 (≠ F73), C101 (≠ A74), A102 (= A75), L104 (≠ I77), N119 (≠ V92), S143 (= S116), S144 (≠ T117), Y169 (= Y141), K173 (= K145), Y196 (= Y168), F197 (= F169), A199 (= A171)
binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: L104 (≠ I77), S144 (≠ T117), Y169 (= Y141), F197 (= F169), N198 (= N170), H217 (= H187), L218 (= L188), P238 (≠ S204), I239 (= I205), F240 (= F206), C251 (≠ A217), R253 (= R219), V297 (= V256), R320 (= R279), D323 (= D282)

1gy8C Trypanosoma brucei udp-galactose 4' epimerase (see paper)
40% identity, 100% coverage: 1:324/325 of query aligns to 3:369/370 of 1gy8C

query
sites
1gy8C

M

M

R

R

V

V

L

L

V

V

T

C

G
|
G

G

G

A

A

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

F

T

V

A

R

A

A

L

L

-

R

G

D

A

T

G

N

H

H

E

S

V

V

T

V

V

I

L

V

D
|
D

D
x
S

L
|
L

-
x
V

-
x
G

S

T

T

H

G

G

H

K

R

S

D

D

A

H

V

V

P

E

-

T

-

R

-

E

-

N

A

V

G

A

A

R

E

K

L

L

V

Q

A

S

D

D

A

G

T

P

D

K

P

P

-

P

-

W

-

A

-

D

-

R

-

Y

-

A

T

L

A

E

V

V

G

G

P
x
D

V
|
V

V

R

G

N

D

E

-

D

-

F

-

L

-

N

-

G

-

V

-

F

-

T

-

R

-

H

G

G

R

P

F

I

D

D

A

A

C

V

I

V

H

H

F
x
M

A
x
C

A
|
A

L

F

I
x
L

E

A

A

V

G

G

E

E

S

S

M

V

R

R

H

D

P

P

E

L

R

K

F

Y

T

D

V
x
N

N

N

T

V

A

V

G

G

T

I

A

L

R

R

L

L

E

Q

V

A

L

M

I

L

D

L

H

H

A

K

V

C

P

D

R

K

F

I

V

I

L

F

S
|
S

T
x
S

A
|
A

V

I

Y

F

G

G

E

N

P

P

E

T

R

M

T

N

-

A

-

E

P

P

I

I

D

D

E

I

D

N

H

A

P

K

L

K

A

S

P

P

T

E

N

S

A

P

Y
|
Y

G

G

E

E

S

S

K
|
K

L

L

L

I

I

A

E

E

R

R

M

M

L

I

A

R

W

D

H

C

H

A

R

E

I

A

H

Y

G

G

L

I

R

K

T

G

A

I

A

C

L

L

R

R

Y
|
Y

F

F

N
|
N

A
|
A

A

C

G

G

A

A

T

H

P

E

G

D

R

G

-

D

-

I

G

G

E

E

R

H

H

Y

D

Q

P

G

E

S

T

T

H
|
H

L
|
L

I

I

P

P

L
x
I

V

I

L

L

-

G

-

R

-

V

-

M

-

S

Q

D

V

I

A

A

-

P

-

D

A

A

G

S

E

T

R

D

D

K

Q

R

V
x
M

S
x
P

I
|
I

F
|
F

G

G

S

T

D

D

Y

Y

P

P

T

T

P

P

D

D

G

G

T

T

A
x
C

I

V

R
|
R

D

D

Y

Y

V

V

H

H

V

V

A

C

D

D

L

L

A

A

D

S

A

A

H

H

V

I

R

L

A

A

V

L

E

D

R

Y

L

V

D

E

Q

K

L

L

G

G

T

P

-

N

-

D

-

K

-

S

-

K

-

Y

-

F

C

S

T

V

V

F

N

N

L

L

G

G

S

T

G

S

T

R

G

G

F

Y

S

S

V

V

R

R

E

E

V

V

I

I

D

E

A

V

A

A

R

R

R

K

V

T

T

T

G

G

H

H

A

P

I

I

P

P

V

V

I

R

E

E

A

C

D

G

R

R

R
|
R

A

E

G

G

D
|
D

P

P

A

A

R

Y

L

L

V

V

A

A

S

A

A

S

T

D

R

K

A

A

R

R

E

E

L

V

L

L

G

G

W

W

E

K

P

P

A

K

I

Y

T

D

D

T

L

L

D

E

R

A

I

I

V

M

A

E

D

T

A

S

W

W

N

K

A

F

T

Q

V

R

D

T

H

H

A

P

G

N

A

G

H

Y

active site: S144 (≠ T117), A145 (= A118), A146 (= A119), Y170 (= Y141), K174 (= K145)
binding nicotinamide-adenine-dinucleotide: G9 (= G7), G12 (= G10), Y13 (≠ F11), I14 (= I12), D34 (= D31), S35 (≠ D32), L36 (= L33), V37 (vs. gap), G38 (vs. gap), D77 (≠ P60), V78 (= V61), M100 (≠ F73), C101 (≠ A74), A102 (= A75), L104 (≠ I77), N119 (≠ V92), S142 (= S115), S143 (= S116), S144 (≠ T117), Y170 (= Y141), K174 (= K145), Y197 (= Y168), A200 (= A171)
binding uridine-5'-diphosphate: N199 (= N170), H218 (= H187), L219 (= L188), I222 (≠ L191), M241 (≠ V203), P242 (≠ S204), I243 (= I205), F244 (= F206), C255 (≠ A217), R257 (= R219), R324 (= R279), D327 (= D282)

8wovB Crystal structure of arabidopsis thaliana udp-glucose 4-epimerase 2 (atuge2) complexed with udp, g233a mutant (see paper)
39% identity, 96% coverage: 3:315/325 of query aligns to 5:332/341 of 8wovB

query
sites
8wovB

V

V

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

T

T

V

A

L

A

Q

L

L

G

E

A

G

G

G

H

Y

E

S

V

A

T

V

V

V

L

V

D
|
D

D
x
N

L

Y

S
x
D

T
x
N

G
x
S

H

S

R

A

D

A

A

S

V

L

P

Q

A

R

G

V

A

K

E

K

L

L

V

A

-

G

-

E

-

N

-

G

-

N

-

R

-

L

-

S

-

F

-

H

V

Q

A

V

D
|
D

A
x
L

T

R

D

D

P

R

T

P

A

A

V

L

G

E

P

K

V

I

V

F

G

S

D

E

G

T

R

K

F

F

D

D

A

A

C

V

I

I

H

H

F
|
F

A
|
A

A
x
G

L

L

I
x
K

E

A

A

V

G

G

E

E

S

S

M

V

R

E

H

K

P

P

E

L

R

L

F

Y

T

N

V
x
N

N

N

T

I

A

V

G

G

T

T

A

V

R

T

L

L

E

E

V

V

L

M

I

A

D

Q

H

Y

A

G

V

C

P

K

R

N

F

L

V

V

L

F

S
|
S

S

S

T
x
S

A

A

A

T

V

V

Y

Y

G

G

E

W

P

P

E

K

R

E

T

V

P

P

I

C

D

T

E

E

D

E

H

S

P

P

L

I

A

S

P

A

T

T

N

N

A

P

Y
|
Y

G

G

E

R

S

T

K
|
K

L

L

L

F

I

I

E

E

R

E

M

I

L

C

A

R

W

D

H

V

H

H

R

R

I

S

H

D

G

S

-

E

L

W

R

K

T

I

A

I

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

A
x
P

A

V

G

G

A

A

T

H

P

P

G

S

R

G

G

Y

E

I

R

G

H

E

D

D

P

P

-

L

-

G

-

V

E

P

T

N

H
x
N

L
|
L

I

M

P

P

L

Y

V

V

L

Q

Q

Q

V

V

A

A

A

V

G

G

E

R

R

R

D

P

Q

H

V
x
L

S
x
T

I

V

F
|
F

G

G

S

T

D

D

Y

Y

P

K

T

T

P

K

D

D

G

G

T

T

A

A

I

V

R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

A

M

D

D

L

L

A

A

D

D

A

G

H

H

V

I

R

A

A

A

V

L

E

R

R

K

L

L

D

D

Q

D

L

L

G

K

-

I

T

S

C

C

T

E

V

V

-

Y

N

N

L

L

G

G

S

T

G

G

T

N

G

G

F

T

S

S

V

V

R

L

E

E

V

M

I

V

D

A

A

A

A

F

R

E

R

K

V

A

T

S

G

G

H

K

A

K

I

I

P

P

V

L

I

V

E

M

A

A

D

G

R

R

R
|
R

A

P

G

G

D
|
D

P

A

A

E

R

V

L

V

V

Y

A

A

S

S

A

T

T

E

R

K

A

A

R

E

E

R

L

E

L

L

G

N

W

W

E

K

P

-

A

A

I

K

T

N

D

G

L

I

D

E

R

E

I

M

V

C

A

R

D

D

A

L

W

W

N

N

binding nicotinamide-adenine-dinucleotide: G9 (= G7), G12 (= G10), Y13 (≠ F11), I14 (= I12), D33 (= D31), N34 (≠ D32), D36 (≠ S34), N37 (≠ T35), S38 (≠ G36), D63 (= D51), L64 (≠ A52), F85 (= F73), A86 (= A74), G87 (≠ A75), K89 (≠ I77), N104 (≠ V92), S127 (= S115), S129 (≠ T117), Y153 (= Y141), K157 (= K145), Y181 (= Y168), P184 (≠ A171)
binding uridine-5'-diphosphate: N183 (= N170), N203 (≠ H187), L204 (= L188), L219 (≠ V203), T220 (≠ S204), F222 (= F206), R235 (= R219), Y237 (= Y221), R297 (= R279), D300 (= D282)

1hzjA Human udp-galactose 4-epimerase: accommodation of udp-n- acetylglucosamine within the active site (see paper)
39% identity, 96% coverage: 2:314/325 of query aligns to 3:333/345 of 1hzjA

query
sites
1hzjA

R

K

V

V

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

T

T

V

A

L

A

E

L

L

G

E

A

A

G

G

H

Y

E

L

V

P

T

V

V

V

L

I

D
|
D

D
x
N

L

F

S
x
H

T
x
N

G

A

H

F

R

R

D

G

-

G

A

S

V

L

P

P

A

E

G

S

-

L

-

R

-

V

-

Q

-

E

-

L

-

T

-

G

-

R

-

S

A

V

E

E

L

F

V

E

A

M

D
|
D

A
x
I

T

L

D

D

P

Q

T

G

A

A

V

L

G

Q

P

R

V

L

V

F

G

K

D

K

G

Y

R

S

F

F

D

M

A

A

C

V

I

I

H

H

F
|
F

A
|
A

A
x
G

L

L

I
x
K

E

A

A

V

G

G

E

E

S

S

M

V

R

Q

H

K

P

P

E

L

R

D

F

Y

T

R

V

V

N

N

T

L

A

T

G

G

T

T

A

I

R

Q

L

L

E

E

V

I

L

M

I

K

D

A

H

H

A

G

V

V

P

K

R

N

F

L

V

V

L

F

S
|
S

S

S

T
x
S

A
|
A

A
x
T

V

V

Y

Y

G

G

E

N

P

P

E

Q

R

Y

T

L

P

P

I

L

D

D

E

E

D

A

H

H

P

P

L

T

-

G

A

G

P

C

T

T

N

N

A

P

Y
|
Y

G

G

E

K

S

S

K
|
K

L

F

I

I

E

E

R

E

M

M

L

I

-

R

-

D

-

L

-

C

-

Q

-

A

-

D

-

K

A

T

W

W

H

N

H

-

R

-

I

-

H

-

G

-

L

-

R

-

T

V

A

V

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

A
x
P

A

T

G

G

A

A

T

H

P

A

G

S

R

G

G

C

E

I

R

G

H

E

D

D

P

P

E

Q

-

G

-

I

-

P

T

N

H
x
N

L
|
L

I

M

P

P

L

Y

V

V

L

S

Q

Q

V

V

A

A

A

I

G

G

E

R

R

R

D

E

Q

A

V

L

S
x
N

I
x
V

F
|
F

G

G

S

N

D

D

Y

Y

P

D

T

T

P

E

D

D

G

G

T

T

A

G

I

V

R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

A

V

D

D

L

L

A

A

D

K

A

G

H

H

V

I

R

A

A

A

V

L

E

R

R

K

L

L

-

K

D

E

Q

Q

L

C

G

G

T

C

C

R

T

I

V

Y

N

N

L

L

G

G

S

T

G

G

T

T

G

G

F

Y

S

S

V
|
V

R

L

E

Q

V

M

I

V

D

Q

A

A

A

M

R

E

R

K

V

A

T

S

G

G

H

K

A

K

I

I

P

P

V

Y

I

K

E

V

A

V

D

A

R

R

R
|
R

A

E

G

G

D
|
D

P

V

A

A

R

A

L

C

V

Y

A

A

S

N

A

P

T

S

R

L

A

A

R

Q

E

E

L

E

L

L

G

G

W

W

E

T

P

A

A

A

I

L

T

-

D

G

L

L

D

D

R

R

I

M

V

C

A

E

D

D

A

L

W

W

active site: S131 (≠ T117), A132 (= A118), T133 (≠ A119), Y156 (= Y141), K160 (= K145)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (≠ F11), I13 (= I12), D32 (= D31), N33 (≠ D32), H35 (≠ S34), N36 (≠ T35), D65 (= D51), I66 (≠ A52), F87 (= F73), A88 (= A74), G89 (≠ A75), K91 (≠ I77), S129 (= S115), S131 (≠ T117), Y156 (= Y141), K160 (= K145), Y184 (= Y168), P187 (≠ A171)
binding uridine-diphosphate-n-acetylglucosamine: N186 (= N170), N206 (≠ H187), L207 (= L188), N223 (≠ S204), V224 (≠ I205), F225 (= F206), R238 (= R219), Y240 (= Y221), V276 (= V256), R299 (= R279), D302 (= D282)

1i3lA Molecular basis for severe epimerase-deficiency galactosemia: x-ray structure of the human v94m-substituted udp-galactose 4-epimerase (see paper)
39% identity, 96% coverage: 2:314/325 of query aligns to 3:333/347 of 1i3lA

query
sites
1i3lA

R

K

V

V

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

T

T

V

A

L

A

E

L

L

G

E

A

A

G

G

H

Y

E

L

V

P

T

V

V

V

L

I

D
|
D

D
x
N

L

F

S
x
H

T
x
N

G

A

H

F

R

R

D

G

-

G

A

S

V

L

P

P

A

E

G

S

-

L

-

R

-

V

-

Q

-

E

-

L

-

T

-

G

-

R

-

S

A

V

E

E

L

F

V

E

A

M

D
|
D

A
x
I

T

L

D

D

P

Q

T

G

A

A

V

L

G

Q

P

R

V

L

V

F

G

K

D

K

G

Y

R

S

F

F

D

M

A

A

C

V

I

I

H

H

F
|
F

A
|
A

A
x
G

L

L

I
x
K

E

A

A

M

G

G

E

E

S

S

M

V

R

Q

H

K

P

P

E

L

R

D

F

Y

T

R

V

V

N

N

T

L

A

T

G

G

T

T

A

I

R

Q

L

L

E

E

V

I

L

M

I

K

D

A

H

H

A

G

V

V

P

K

R

N

F

L

V

V

L

F

S
|
S

T
x
S

A
|
A

A
x
T

V

V

Y

Y

G

G

E

N

P

P

E

Q

R

Y

T

L

P

P

I

L

D

D

E

E

D

A

H

H

P

P

L

T

-

G

A

G

P

C

T

T

N

N

A

P

Y
|
Y

G

G

E

K

S

S

K
|
K

L

F

I

I

E

E

R

E

M

M

L

I

-

R

-

D

-

L

-

C

-

Q

-

A

-

D

-

K

A

T

W

W

H

N

H

-

R

-

I

-

H

-

G

-

L

-

R

-

T

V

A

V

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

A
x
P

A

T

G

G

A

A

T

H

P

A

G

S

R

G

G

C

E

I

R

G

H

E

D

D

P

P

E

Q

-

G

-

I

-

P

T

N

H
x
N

L
|
L

I

M

P

P

L

Y

V

V

L

S

Q

Q

V

V

A

A

A

I

G

G

E

R

R

R

D

E

Q

A

V

L

S
x
N

I
x
V

F
|
F

G

G

S

N

D

D

Y

Y

P

D

T

T

P

E

D

D

G

G

T

T

A

G

I

V

R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

A

V

D

D

L

L

A

A

D

K

A

G

H

H

V

I

R

A

A

A

V

L

E

R

R

K

L

L

-

K

D

E

Q

Q

L

C

G

G

T

C

C

R

T

I

V

Y

N

N

L

L

G

G

S

T

G

G

T

T

G

G

F

Y

S

S

V
|
V

R

L

E

Q

V

M

I

V

D

Q

A

A

A

M

R

E

R

K

V

A

T

S

G

G

H

K

A

K

I

I

P

P

V

Y

I

K

E

V

A

V

D

A

R

R

R
|
R

A

E

G

G

D
|
D

P

V

A

A

R

A

L

C

V

Y

A

A

S

N

A

P

T

S

R

L

A

A

R

Q

E

E

L

E

L

L

G

G

W

W

E

T

P

A

A

A

I

L

T

-

D

G

L

L

D

D

R

R

I

M

V

C

A

E

D

D

A

L

W

W

active site: S131 (≠ T117), A132 (= A118), T133 (≠ A119), Y156 (= Y141), K160 (= K145)
binding galactose-uridine-5'-diphosphate: N186 (= N170), N206 (≠ H187), L207 (= L188), N223 (≠ S204), V224 (≠ I205), F225 (= F206), R238 (= R219), Y240 (= Y221), V276 (= V256), R299 (= R279), D302 (= D282)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (≠ F11), I13 (= I12), D32 (= D31), N33 (≠ D32), H35 (≠ S34), N36 (≠ T35), D65 (= D51), I66 (≠ A52), F87 (= F73), A88 (= A74), G89 (≠ A75), K91 (≠ I77), S129 (= S115), S130 (= S116), S131 (≠ T117), Y156 (= Y141), K160 (= K145), Y184 (= Y168), P187 (≠ A171)

1i3kA Molecular basis for severe epimerase-deficiency galactosemia: x-ray structure of the human v94m-substituted udp-galactose 4-epimerase (see paper)
39% identity, 96% coverage: 2:314/325 of query aligns to 3:333/347 of 1i3kA

query
sites
1i3kA

R

K

V

V

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

T

T

V

A

L

A

E

L

L

G

E

A

A

G

G

H

Y

E

L

V

P

T

V

V

V

L

I

D
|
D

D
x
N

L

F

S
x
H

T
x
N

G

A

H

F

R

R

D

G

-

G

A

S

V

L

P

P

A

E

G

S

-

L

-

R

-

V

-

Q

-

E

-

L

-

T

-

G

-

R

-

S

A

V

E

E

L

F

V

E

A

M

D
|
D

A
x
I

T

L

D

D

P

Q

T

G

A

A

V

L

G

Q

P

R

V

L

V

F

G

K

D

K

G

Y

R

S

F

F

D

M

A

A

C

V

I

I

H

H

F
|
F

A
|
A

A
x
G

L

L

I
x
K

E

A

A

M

G

G

E

E

S

S

M

V

R

Q

H

K

P

P

E

L

R

D

F

Y

T

R

V

V

N

N

T

L

A

T

G

G

T

T

A

I

R

Q

L

L

E

E

V

I

L

M

I

K

D

A

H

H

A

G

V

V

P

K

R

N

F

L

V

V

L

F

S
|
S

S

S

T
x
S

A
|
A

A
x
T

V

V

Y

Y

G

G

E

N

P

P

E

Q

R

Y

T

L

P

P

I

L

D

D

E

E

D

A

H

H

P

P

L

T

-

G

A

G

P

C

T

T

N

N

A

P

Y
|
Y

G

G

E

K

S

S

K
|
K

L

F

I

I

E

E

R

E

M

M

L

I

-

R

-

D

-

L

-

C

-

Q

-

A

-

D

-

K

A

T

W

W

H

N

H

-

R

-

I

-

H

-

G

-

L

-

R

-

T

V

A

V

A

L

L

L

R

R

Y
|
Y

F
|
F

N
|
N

A
x
P

A

T

G

G

A

A

T

H

P

A

G

S

R

G

G

C

E

I

R

G

H

E

D

D

P

P

E

Q

-

G

-

I

-

P

T

N

H
x
N

L
|
L

I

M

P

P

L

Y

V

V

L

S

Q

Q

V

V

A

A

A

I

G

G

E

R

R

R

D

E

Q

A

V

L

S
x
N

I
x
V

F
|
F

G

G

S

N

D

D

Y

Y

P

D

T

T

P

E

D

D

G

G

T

T

A

G

I

V

R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

A

V

D

D

L

L

A

A

D

K

A

G

H

H

V

I

R

A

A

A

V

L

E

R

R

K

L

L

-

K

D

E

Q

Q

L

C

G

G

T

C

C

R

T

I

V

Y

N

N

L

L

G

G

S

T

G

G

T

T

G

G

F

Y

S

S

V
|
V

R

L

E

Q

V

M

I

V

D

Q

A

A

A

M

R

E

R

K

V

A

T

S

G

G

H

K

A

K

I

I

P

P

V

Y

I

K

E

V

A

V

D

A

R

R

R
|
R

A

E

G

G

D
|
D

P

V

A

A

R

A

L

C

V

Y

A

A

S

N

A

P

T

S

R

L

A

A

R

Q

E

E

L

E

L

L

G

G

W

W

E

T

P

A

A

A

I

L

T

-

D

G

L

L

D

D

R

R

I

M

V

C

A

E

D

D

A

L

W

W

active site: S131 (≠ T117), A132 (= A118), T133 (≠ A119), Y156 (= Y141), K160 (= K145)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (≠ F11), I13 (= I12), D32 (= D31), N33 (≠ D32), H35 (≠ S34), N36 (≠ T35), D65 (= D51), I66 (≠ A52), F87 (= F73), A88 (= A74), G89 (≠ A75), K91 (≠ I77), S129 (= S115), S131 (≠ T117), Y156 (= Y141), K160 (= K145), Y184 (= Y168), P187 (≠ A171)
binding uridine-5'-diphosphate-glucose: F185 (= F169), N186 (= N170), N206 (≠ H187), L207 (= L188), N223 (≠ S204), V224 (≠ I205), F225 (= F206), R238 (= R219), Y240 (= Y221), V276 (= V256), R299 (= R279), D302 (= D282)

3enkA 1.9a crystal structure of udp-glucose 4-epimerase from burkholderia pseudomallei
42% identity, 96% coverage: 3:314/325 of query aligns to 7:329/340 of 3enkA

query
sites
3enkA

V

I

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

T

T

A

A

V

A

E

L

L

G

A

A

H

G

G

H

Y

E

D

V

V

T

V

V

I

L

A

D
|
D

D
x
N

L

L

S
x
V

T
x
N

G
x
S

H

K

R

R

D

E

A

A

V

I

-

A

-

R

-

I

-

E

-

K

-

I

-

T

-

G

-

K

-

T

P

P

A

A

G

F

A

H

E

E

L

-

V

-

A

T

D
|
D

A
x
V

T

S

D

D

P

E

T

R

A

A

V

L

G

A

P

R

V

I

V

F

G

D

D

A

G

H

R

P

F

I

D

T

A

A

C

A

I

I

H

H

F
|
F

A
|
A

L

L

I
x
K

E

A

A

V

G

G

E

E

S

S

M

V

R

A

H

K

P

P

E

I

R

E

F

Y

T

R

V
x
N

N

N

T

L

A

D

G

S

T

L

A

L

R

S

L

L

E

R

V

V

L

M

I

R

D

E

H

R

A

A

V

V

P

K

R

R

F

I

V

V

L

F

S
|
S

T
x
S

A

A

A
x
T

V

V

Y

Y

G

G

E

V

P

P

E

E

R

R

T

S

P

P

I

I

D

D

E

E

D

T

H

F

P

P

L

L

A

S

P

A

T

T

N

N

A

P

Y
|
Y

G

G

E

Q

S

T

K
|
K

L

L

L

M

I

A

E

E

R

Q

M

I

L

L

-

R

-

D

-

V

-

E

-

A

-

D

-

P

A

S

W

W

H

-

R

-

I

-

H

-

G

-

L

-

R

R

T

V

A

A

A

T

L

L

R

R

Y
|
Y

F

F

N
|
N

A
x
P

A

V

G

G

A

A

T

H

P

E

G

S

R

G

G

L

E

I

R

G

H

E

D

D

P

P

E

A

-

G

-

I

-

P

T
x
N

H
x
N

L
|
L

I

M

P

P

L

Y

V

V

L

A

Q

Q

V

V

A

A

A

V

G

G

E

K

R

L

D

E

Q

K

V

L

S
x
R

I
x
V

F
|
F

G

G

S

S

D

D

Y

Y

P

P

T

T

P

P

D

D

G

G

T

T

A

G

I

V

R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

A

V

D

D

L

L

A

A

D

R

A

G

H

H

V

I

R

A

A

A

V

L

E

D

R

A

L

L

D

E

Q

R

L

R

-

D

G

A

T

S

C

L

T

T

V

V

N

N

L

L

G

G

S

T

G

G

T

R

G

G

F

Y

S

S

V
|
V

R

L

E

E

V

V

I

V

D

R

A

A

A

F

R

E

R

K

V

A

T

S

G

G

H

R

A

A

I

V

P

P

V

Y

I

E

E

L

A

V

D

A

R

R

R
|
R

A

P

G

G

D
|
D

P

V

A

A

R

E

L

C

V

Y

A

A

S

N

A

P

T

A

R

A

A

A

R

A

E

E

L

T

L

I

G

G

W

W

E

K

P

-

A

A

I

E

T

R

D

D

L

L

D

E

R

R

I

M

V

C

A

A

D

D

A

H

W

W

active site: S127 (= S116), S128 (≠ T117), T130 (≠ A119), Y152 (= Y141), K156 (= K145)
binding nicotinamide-adenine-dinucleotide: G11 (= G7), G14 (= G10), Y15 (≠ F11), I16 (= I12), D35 (= D31), N36 (≠ D32), V38 (≠ S34), N39 (≠ T35), S40 (≠ G36), D62 (= D51), V63 (≠ A52), F84 (= F73), A85 (= A74), A86 (= A75), K88 (≠ I77), N103 (≠ V92), S126 (= S115), S128 (≠ T117), Y152 (= Y141), K156 (= K145), Y180 (= Y168), P183 (≠ A171)
binding uridine-5'-diphosphate-glucose: T130 (≠ A119), N182 (= N170), N201 (≠ T186), N202 (≠ H187), L203 (= L188), R219 (≠ S204), V220 (≠ I205), F221 (= F206), R234 (= R219), Y236 (= Y221), V272 (= V256), R295 (= R279), D298 (= D282)

1ek6A Structure of human udp-galactose 4-epimerase complexed with nadh and udp-glucose (see paper)
39% identity, 96% coverage: 2:314/325 of query aligns to 4:334/346 of 1ek6A

query
sites
1ek6A

R

K

V

V

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

T

T

V

A

L

A

E

L

L

G

E

A

A

G

G

H

Y

E

L

V

P

T

V

V

V

L

I

D
|
D

D
x
N

L

F

S
x
H

T
x
N

G

A

H

F

R

R

D

G

-

G

A

S

V

L

P

P

A

E

G

S

-

L

-

R

-

V

-

Q

-

E

-

L

-

T

-

G

-

R

-

S

A

V

E

E

L

F

V

E

A

M

D
|
D

A
x
I

T

L

D

D

P

Q

T

G

A

A

V

L

G

Q

P

R

V

L

V

F

G

K

D

K

G

Y

R

S

F

F

D

M

A

A

C

V

I

I

H

H

F
|
F

A
|
A

A
x
G

L

L

I
x
K

E

A

A

V

G

G

E

E

S

S

M

V

R

Q

H

K

P

P

E

L

R

D

F

Y

T

R

V

V

N

N

T

L

A

T

G

G

T

T

A

I

R

Q

L

L

E

E

V

I

L

M

I

K

D

A

H

H

A

G

V

V

P

K

R

N

F

L

V

V

L

F

S
|
S

T
x
S

A
|
A

A
x
T

V

V

Y

Y

G

G

E

N

P

P

E

Q

R

Y

T

L

P

P

I

L

D

D

E

E

D

A

H

H

P

P

L

T

-

G

A

G

P

C

T

T

N

N

A

P

Y
|
Y

G

G

E

K

S

S

K
|
K

L

F

I

I

E

E

R

E

M

M

L

I

-

R

-

D

-

L

-

C

-

Q

-

A

-

D

-

K

A

T

W

W

H

N

H

-

R

-

I

-

H

-

G

-

L

-

R

-

T

A

A

V

A

L

L

L

R

R

Y
|
Y

F
|
F

N
|
N

A
x
P

A

T

G

G

A

A

T

H

P

A

G

S

R

G

G

C

E

I

R

G

H

E

D

D

P

P

E

Q

-

G

-

I

-

P

T

N

H
x
N

L
|
L

I

M

P

P

L

Y

V

V

L

S

Q

Q

V

V

A

A

A

I

G

G

E

R

R

R

D

E

Q

A

V

L

S
x
N

I
x
V

F
|
F

G

G

S

N

D

D

Y

Y

P

D

T

T

P

E

D

D

G

G

T

T

A

G

I

V

R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

A

V

D

D

L

L

A

A

D

K

A

G

H

H

V

I

R

A

A

A

V

L

E

R

R

K

L

L

-

K

D

E

Q

Q

L

C

G

G

T

C

C

R

T

I

V

Y

N

N

L

L

G

G

S

T

G

G

T

T

G

G

F

Y

S

S

V
|
V

R

L

E

Q

V

M

I

V

D

Q

A

A

A

M

R

E

R

K

V

A

T

S

G

G

H

K

A

K

I

I

P

P

V

Y

I

K

E

V

A

V

D

A

R

R

R
|
R

A

E

G

G

D
|
D

P

V

A

A

R

A

L

C

V

Y

A

A

S

N

A

P

T

S

R

L

A

A

R

Q

E

E

L

E

L

L

G

G

W

W

E

T

P

A

A

A

I

L

T

-

D

G

L

L

D

D

R

R

I

M

V

C

A

E

D

D

A

L

W

W

active site: S132 (≠ T117), A133 (= A118), T134 (≠ A119), Y157 (= Y141), K161 (= K145)
binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G7), G12 (= G10), Y13 (≠ F11), I14 (= I12), D33 (= D31), N34 (≠ D32), H36 (≠ S34), N37 (≠ T35), D66 (= D51), I67 (≠ A52), F88 (= F73), A89 (= A74), G90 (≠ A75), K92 (≠ I77), S130 (= S115), S131 (= S116), S132 (≠ T117), Y157 (= Y141), K161 (= K145), Y185 (= Y168), P188 (≠ A171)
binding uridine-5'-diphosphate-glucose: S132 (≠ T117), Y157 (= Y141), F186 (= F169), N187 (= N170), N207 (≠ H187), L208 (= L188), N224 (≠ S204), V225 (≠ I205), F226 (= F206), R239 (= R219), Y241 (= Y221), V277 (= V256), R300 (= R279), D303 (= D282)

Q14376 UDP-glucose 4-epimerase; Galactowaldenase; UDP-N-acetylgalactosamine 4-epimerase; UDP-GalNAc 4-epimerase; UDP-N-acetylglucosamine 4-epimerase; UDP-GlcNAc 4-epimerase; UDP-galactose 4-epimerase; EC 5.1.3.2; EC 5.1.3.7 from Homo sapiens (Human) (see 18 papers)
39% identity, 96% coverage: 2:314/325 of query aligns to 4:334/348 of Q14376

query
sites
Q14376

R

K

V

V

L

L

V

V

T

T

G

G

A

A

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

T

T

V

A

L

A

E

L

L

G

E

A

A

G

G

H

Y

E

L

V

P

T

V

V

V

L

I

D
|
D

D
x
N

L
x
F

S
x
H

T
x
N

G

A

H

F

R

R

D

G

-

G

A

S

V

L

P

P

A

E

G

S

-

L

-

R

-
x
R

-

V

-

Q

-

E

-

L

-

T

-

G

-

R

-

S

A

V

E

E

L

F

V

E

A

M

D
|
D

A
x
I

T

L

D

D

P

Q

T

G

A

A

V

L

G

Q

P

R

V

L

V

F

G

K

D

K

G

Y

R
x
S

F

F

D

M

A

A

C

V

I

I

H

H

F
|
F

A

A

A
x
G

L

L

I
x
K

E

A

A
x
V

G

G

E

E

S

S

M

V

R

Q

H

K

P

P

E

L

R
x
D

F

Y

T

R

V

V

N

N

T

L

A

T

G

G

T

T

A

I

R

Q

L

L

E

E

V

I

L

M

I

K

D

A

H

H

A

G

V

V

P

K

R

N

F

L

V
|
V

L

F

S

S

T
x
S

A
|
A

A
x
T

V

V

Y

Y

G

G

E

N

P

P

E

Q

R

Y

T

L

P

P

I

L

D

D

E

E

D

A

H

H

P

P

L
x
T

-

G

A

G

P

C

T

T

N

N

A

P

Y
|
Y

G

G

E

K

S

S

K
|
K

L

F

I

I

E

E

R

E

M

M

L

I

-

R

-

D

-

L

-

C

-

Q

-

A

-

D

-

K

A

T

W

W

H

N

H

-

R

-

I

-

H

-

G

-

L

-

R

-

T
x
A

A

V

A

L

L
|
L

R

R

Y
|
Y

F
|
F

N
|
N

A

P

A

T

G

G

A

A

T

H

P

A

G

S

R

G

G

C

E

I

R

G

H

E

D

D

P

P

E

Q

-

G

-

I

-

P

T
x
N

H
x
N

L
|
L

I

M

P

P

L

Y

V

V

L

S

Q

Q

V

V

A

A

A

I

G

G

E

R

R

R

D

E

Q

A

V
x
L

S
x
N

I
x
V

F
|
F

G

G

S

N

D

D

Y

Y

P

D

T

T

P

E

D

D

G

G

T

T

A
x
G

I

V

R
|
R

D

D

Y

Y

V

I

H

H

V

V

A

V

D

D

L

L

A

A

D

K

A

G

H

H

V

I

R

A

A

A

V

L

E

R

R
x
K

L

L

-

K

D

E

Q

Q

L

C

G

G

T

C

C

R

T

I

V

Y

N

N

L

L

G

G

S

T

G

G

T

T

G

G

F

Y

S

S

V

V

R

L

E

Q

V

M

I

V

D

Q

A

A

A

M

R

E

R

K

V

A

T

S

G

G

H

K

A

K

I

I

P

P

V

Y

I

K

E

V

A

V

D

A

R

R

R
|
R

A
x
E

G
|
G

D
|
D

P

V

A

A

R

A

L
x
C

V

Y

A

A

S

N

A

P

T

S

R
x
L

A

A

R

Q

E

E

L

E

L

L

G
|
G

W

W

E

T

P

A

A

A

I

L

T

-

D

G

L

L

D

D

R

R

I

M

V

C

A

E

D

D

A

L

W

W

GYI 12:14 (≠ GFI 10:12) binding NAD(+)
DNFHN 33:37 (≠ DDLST 31:35) binding NAD(+)
N34 (≠ D32) to S: in GALAC3; peripheral; nearly normal activity towards UDP-galactose; dbSNP:rs121908046
R51 (vs. gap) to W: in THC13; likely pathogenic; decreased UDP-galactose epimerization activity; decreased UDP-N-acetylglucosamine epimerase activity; reduced NAD+ binding; decreased thermal stability
DI 66:67 (≠ DA 51:52) binding NAD(+)
S81 (≠ R66) mutation to R: 5-fold decreased catalytic efficiency for UDP-galactose epimerization.
F88 (= F73) binding NAD(+)
G90 (≠ A75) to E: in GALAC3; 800-fold decrease in UDP-galactose epimerization activity; dbSNP:rs28940882
K92 (≠ I77) binding NAD(+)
V94 (≠ A79) to M: in GALAC3; generalized; 30-fold decrease in UDP-galactose epimerization activity; 2-fold decrease in affinity for UDP-galactose; 24% of normal activity with respect to UDP-N-acetylgalactosamine; dbSNP:rs121908047
D103 (≠ R88) to G: in GALAC3; uncertain significance; 7-fold decrease in UDP-galactose epimerization activity; very mild decrease in activity towards UDP-N-acetylgalactosamine; dbSNP:rs28940883
V128 (= V113) to M: in THC13; likely pathogenic
S132 (≠ T117) mutation to A: Loss of activity.
SAT 132:134 (≠ TAA 117:119) binding substrate
T150 (≠ L135) to M: in THC13; likely pathogenic; 3-fold decreased catalytic efficiency for UDP-galactose epimerization
Y157 (= Y141) active site, Proton acceptor; mutation to F: Loss of activity.
K161 (= K145) binding NAD(+)
A180 (≠ T163) to V: in dbSNP:rs3204468
L183 (= L166) to P: in GALAC3; peripheral; 3-fold decrease in UDP-galactose epimerization activity; dbSNP:rs121908045
Y185 (= Y168) binding NAD(+)
YFN 185:187 (= YFN 168:170) binding substrate
NNL 206:208 (≠ THL 186:188) binding substrate
L223 (≠ V203) to P: in THC13; likely pathogenic
NVF 224:226 (≠ SIF 204:206) binding substrate
G237 (≠ A217) to D: in THC13; uncertain significance
R239 (= R219) binding substrate
K257 (≠ R237) to R: in GALAC3; 7-fold decrease in UDP-galactose epimerization activity; does not affect affinity for UDP-galactose; dbSNP:rs28940884
REGD 300:303 (≠ RAGD 279:282) binding substrate
C307 (≠ L286) mutation to Y: No effect on activity towards UDP-galactose. Loss of activity towards UDP-N-acetylgalactosamine.
L313 (≠ R292) to M: in GALAC3; 6-fold decrease in UDP-galactose epimerization activity; very mild decrease in activity towards UDP-N-acetylgalactosamine; dbSNP:rs3180383
G319 (= G298) to E: in GALAC3; nearly normal activity towards UDP-galactose; mild impairment under conditions of substrate limitation; dbSNP:rs28940885

Sites not aligning to the query:

335 R → H: in GALAC3; 2-fold decrease in UDP-galactose epimerization activity; dbSNP:rs368637540

2udpA Udp-galactose 4-epimerase complexed with udp-phenol (see paper)
39% identity, 100% coverage: 1:325/325 of query aligns to 1:337/338 of 2udpA

query
sites
2udpA

M

M

R

R

V

V

L

L

V

V

T

T

G
|
G

G

G

A

S

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

T

T

C

A

V

A

Q

L

L

G

Q

A

N

G

G

H

H

E

D

V

V

T

I

V

I

L

L

D
|
D

D
x
N

L
|
L

S
x
C

T
x
N

G
x
S

H

K

R

R

D

S

A

V

V

L

P

P

A

V

G

I

A

E

E

R

L

L

-

G

-

K

-

H

-

P

-

T

-

F

V

V

V

E

A

G

D
|
D

A
x
I

T

R

D

N

P

E

T

A

A

L

V

M

G

T

P

E

V

I

V

L

G

H

D

D

G

H

R

A

F

I

D

D

A

T

C

V

I

I

H

H

F
|
F

A
|
A

A
x
G

L

L

I
x
K

E

A

A

V

G

G

E

E

S

S

M

V

R

Q

H

K

P

P

E

L

R

E

F

Y

T

D

V

N

N

N

T

V

A

N

G

G

T

T

A

L

R

R

L

L

L

I

E

S

V

A

L

M

I

R

D

A

H

A

A

N

V

V

P

K

R

N

F

F

V

I

L

F

S
|
S

S

S

T
x
S

A
|
A

A
x
T

V

V

Y

Y

G

G

E

D

P

Q

E

P

R

K

T

I

P

P

I

Y

D

V

E

E

D

S

H

F

P

P

L

T

A

G

-

T

P

P

T

Q

N

S

A

P

Y
|
Y

G

G

E

K

S

S

K
|
K

L

L

L

M

I

V

E

E

R

Q

M

I

L

L

A

T

W

D

H

L

H

Q

R

K

I

A

H

Q

-

P

G

D

L

W

R

S

T

I

A

A

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

A

P

A

V

G

G

A

A

T

H

P

P

G

S

R

G

G

D

E
x
M

R

G

H

E

D

D

P

P

E

Q

-

G

-

I

-

P

T

N

H
x
N

L
|
L

I

M

P

P

L

Y

V

I

L

A

Q

Q

V

V

A

A

A

V

G

G

E

R

R

R

D

D

Q

S

V

L

S
x
A

I
|
I

F
|
F

G

G

S

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

A

G

I

V

R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

A

M

D

D

L

L

A

A

D

D

A

G

H

H

V

V

R

V

A

A

V

M

E

E

R

K

L

L

-

A

D

N

Q

K

L

P

G

G

T

V

C

H

T

I

V

Y

N

N

L

L

G

G

S

A

G

G

T

V

G

G

F

N

S

S

V
|
V

R

L

E

D

V

V

I

V

D

N

A

A

A

F

R

S

R

K

V

A

T

C

G

G

H

K

A

P

I

V

P

N

V

Y

I

H

E

F

A

A

D

P

R

R

R
|
R

A

E

G

G

D
|
D

P

L

A

P

R

A

L

Y

V

W

A

A

S

D

A

A

T

S

R

K

A

A

R

D

E

R

L

E

L

L

G

N

W

W

E

R

P

V

A

T

I

R

T

T

D

-

L

L

D

D

R

E

I

M

V

A

A

Q

D

D

A

T

W

W

N

H

A

W

T

Q

V

S

D

R

H

H

A

P

G

Q

A

G

H

Y

P

P

active site: S124 (≠ T117), A125 (= A118), T126 (≠ A119), Y149 (= Y141), K153 (= K145), M189 (≠ E180)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (= I12), D31 (= D31), N32 (≠ D32), L33 (= L33), C34 (≠ S34), N35 (≠ T35), S36 (≠ G36), D58 (= D51), I59 (≠ A52), F80 (= F73), A81 (= A74), G82 (≠ A75), K84 (≠ I77), S122 (= S115), S124 (≠ T117), Y149 (= Y141), K153 (= K145), Y177 (= Y168)
binding phenyl-uridine-5'-diphosphate: N179 (= N170), N199 (≠ H187), L200 (= L188), A216 (≠ S204), I217 (= I205), F218 (= F206), R231 (= R219), Y233 (= Y221), V269 (= V256), R292 (= R279), D295 (= D282)

1udcA Structure of udp-galactose-4-epimerase complexed with udp-mannose (see paper)
39% identity, 100% coverage: 1:325/325 of query aligns to 1:337/338 of 1udcA

query
sites
1udcA

M

M

R

R

V

V

L

L

V

V

T

T

G
|
G

G

G

A

S

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

T

T

C

A

V

A

Q

L

L

G

Q

A

N

G

G

H

H

E

D

V

V

T

I

V

I

L

L

D
|
D

D
x
N

L

L

S
x
C

T
x
N

G
x
S

H

K

R

R

D

S

A

V

V

L

P

P

A

V

G

I

A

E

E

R

L

L

-

G

-

K

-

H

-

P

-

T

-

F

V

V

V

E

A

G

D
|
D

A
x
I

T

R

D

N

P

E

T

A

A

L

V

M

G

T

P

E

V

I

V

L

G

H

D

D

G

H

R

A

F

I

D

D

A

T

C

V

I

I

H

H

F
|
F

A
|
A

A
x
G

L

L

I
x
K

E

A

A

V

G

G

E

E

S

S

M

V

R

Q

H

K

P

P

E

L

R

E

F

Y

T

D

V

N

N

N

T

V

A

N

G

G

T

T

A

L

R

R

L

L

L

I

E

S

V

A

L

M

I

R

D

A

H

A

A

N

V

V

P

K

R

N

F

F

V

I

L

F

S
|
S

S

S

T
x
S

A
|
A

A
x
T

V

V

Y

Y

G

G

E

D

P

Q

E

P

R

K

T

I

P

P

I

Y

D

V

E

E

D

S

H

F

P

P

L

T

A

G

-

T

P

P

T

Q

N

S

A

P

Y
|
Y

G

G

E

K

S

S

K
|
K

L

L

L

M

I

V

E

E

R

Q

M

I

L

L

A

T

W

D

H

L

H

Q

R

K

I

A

H

Q

-

P

G

D

L

W

R

S

T

I

A

A

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

A
x
P

A

V

G

G

A

A

T

H

P

P

G

S

R

G

G

D

E
x
M

R

G

H

E

D

D

P

P

E

Q

-

G

-

I

-

P

T

N

H
x
N

L
|
L

I

M

P

P

L

Y

V

I

L

A

Q

Q

V

V

A

A

A

V

G

G

E

R

R

R

D

D

Q

S

V
x
L

S
x
A

I
|
I

F
|
F

G

G

S

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

A

G

I

V

R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

A

M

D

D

L

L

A

A

D

D

A

G

H

H

V

V

R

V

A

A

V

M

E

E

R

K

L

L

-

A

D

N

Q

K

L

P

G

G

T

V

C

H

T

I

V

Y

N

N

L

L

G

G

S

A

G

G

T

V

G

G

F

N

S

S

V
|
V

R

L

E

D

V

V

I

V

D

N

A

A

A

F

R

S

R

K

V

A

T

C

G

G

H

K

A

P

I

V

P

N

V

Y

I

H

E

F

A

A

D

P

R

R

R
|
R

A

E

G

G

D

D

P

L

A

P

R

A

L

Y

V

W

A

A

S

D

A

A

T

S

R

K

A

A

R

D

E

R

L

E

L

L

G

N

W

W

E

R

P

V

A

T

I

R

T

T

D

-

L

L

D

D

R

E

I

M

V

A

A

Q

D

D

A

T

W

W

N

H

A

W

T

Q

V

S

D

R

H

H

A

P

G

Q

A

G

H

Y

P

P

active site: S124 (≠ T117), A125 (= A118), T126 (≠ A119), Y149 (= Y141), K153 (= K145), M189 (≠ E180)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (= I12), D31 (= D31), N32 (≠ D32), C34 (≠ S34), N35 (≠ T35), S36 (≠ G36), D58 (= D51), I59 (≠ A52), F80 (= F73), A81 (= A74), G82 (≠ A75), K84 (≠ I77), S122 (= S115), Y149 (= Y141), K153 (= K145), Y177 (= Y168), P180 (≠ A171)
binding uridine-5'-diphosphate-mannose: T126 (≠ A119), Y149 (= Y141), N179 (= N170), N199 (≠ H187), L200 (= L188), L215 (≠ V203), A216 (≠ S204), I217 (= I205), F218 (= F206), R231 (= R219), Y233 (= Y221), V269 (= V256), R292 (= R279)

P09147 UDP-glucose 4-epimerase; Galactowaldenase; UDP-galactose 4-epimerase; EC 5.1.3.2 from Escherichia coli (strain K12) (see 10 papers)
39% identity, 100% coverage: 1:325/325 of query aligns to 1:337/338 of P09147

query
sites
P09147

M

M

R

R

V

V

L

L

V

V

T

T

G

G

A

S

G

G

F
x
Y

I
|
I

G

G

A

S

T

H

T

T

T

C

A

V

A

Q

L

L

G

Q

A

N

G

G

H

H

E

D

V

V

T

I

V

I

L

L

D
|
D

D
x
N

L
|
L

S
x
C

T
x
N

G
x
S

H

K

R

R

D

S

A

V

V

L

P

P

A

V

G

I

A

E

E

R

L

L

-

G

-

K

-

H

-

P

-

T

-

F

V

V

V

E

A

G

D
|
D

A
x
I

T

R

D

N

P

E

T

A

A

L

V

M

G

T

P

E

V

I

V

L

G

H

D

D

G

H

R

A

F

I

D

D

A

T

C

V

I

I

H

H

F
|
F

A
|
A

A
x
G

L
|
L

I
x
K

E

A

A

V

G

G

E

E

S

S

M

V

R

Q

H

K

P

P

E

L

R

E

F

Y

T

D

V
x
N

N

N

T

V

A

N

G

G

T

T

A

L

R

R

L

L

L

I

E

S

V

A

L

M

I

R

D

A

H

A

A

N

V

V

P

K

R

N

F

F

V

I

L

F

S

S

T
x
S

A

A

A

T

V

V

Y

Y

G

G

E

D

P

Q

E

P

R

K

T

I

P

P

I

Y

D

V

E

E

D

S

H

F

P

P

L

T

A

G

-

T

P

P

T

Q

N

S

A

P

Y
|
Y

G

G

E

K

S

S

K
|
K

L

L

L

M

I

V

E

E

R

Q

M

I

L

L

A

T

W

D

H

L

H

Q

R

K

I

A

H

Q

-

P

G

D

L

W

R

S

T

I

A

A

A

L

L

L

R

R

Y

Y

F
|
F

N

N

A

P

A

V

G

G

A

A

T

H

P

P

G

S

R

G

G

D

E

M

R

G

H

E

D

D

P

P

E

Q

-

G

-

I

-

P

T

N

H

N

L

L

I

M

P

P

L

Y

V

I

L

A

Q

Q

V

V

A

A

A

V

G

G

E

R

R

R

D

D

Q

S

V

L

S

A

I

I

F

F

G

G

S

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

A

G

I

V

R

R

D

D

Y

Y

V

I

H

H

V

V

A

M

D

D

L

L

A

A

D

D

A

G

H

H

V

V

R

V

A

A

V

M

E

E

R

K

L

L

-

A

D

N

Q

K

L

P

G

G

T

V

C

H

T

I

V

Y

N

N

L

L

G

G

S

A

G

G

T

V

G

G

F

N

S

S

V

V

R

L

E

D

V

V

I

V

D

N

A

A

A

F

R

S

R

K

V

A

T

C

G

G

H

K

A

P

I

V

P

N

V

Y

I

H

E

F

A

A

D

P

R

R

A

E

G

G

D

D

P

L

A

P

R

A

L
x
Y

V

W

A

A

S

D

A

A

T

S

R

K

A

A

R

D

E

R

L

E

L

L

G

N

W

W

E

R

P

V

A

T

I

R

T

T

D

-

L

L

D

D

R

E

I

M

V

A

A

Q

D

D

A

T

W

W

N

H

A

W

T

Q

V

S

D

R

H

H

A

P

G

Q

A

G

H

Y

P

P

YI 11:12 (≠ FI 11:12) binding NAD(+)
DNLCNS 31:36 (≠ DDLSTG 31:36) binding NAD(+)
DI 58:59 (≠ DA 51:52) binding NAD(+)
FAGLK 80:84 (≠ FAALI 73:77) binding NAD(+)
N99 (≠ V92) binding NAD(+)
S124 (≠ T117) binding NAD(+); mutation to A: No major structural changes. Catalytic efficiency is very low and affinity binding is 21% of the wild-type enzyme.; mutation to T: No major structural changes. Catalytic efficiency is about 30% of that of the wild-type enzyme, and affinity binding is similar to that of the native enzyme.
Y149 (= Y141) active site, Proton acceptor; binding NAD(+); mutation to F: No major structural changes. Catalytic efficiency is very low and affinity binding is 12% of the wild-type enzyme.
K153 (= K145) binding NAD(+); mutation to A: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.; mutation to M: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.
F178 (= F169) binding NAD(+)
Y299 (≠ L286) mutation to C: Loss of epimerase activity with UDP-Gal by almost 5-fold, but it results in a gain of epimerase activity with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) by 230-fold with minimal changes in its three-dimensional structure.

1udaA Structure of udp-galactose-4-epimerase complexed with udp-4-deoxy-4- fluoro-alpha-d-galactose (see paper)
39% identity, 100% coverage: 1:325/325 of query aligns to 1:337/338 of 1udaA

query
sites
1udaA

M

M

R

R

V

V

L

L

V

V

T

T

G
|
G

G

G

A

S

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

T

T

C

A

V

A

Q

L

L

G

Q

A

N

G

G

H

H

E

D

V

V

T

I

V

I

L

L

D
|
D

D
x
N

L
|
L

S
x
C

T
x
N

G
x
S

H

K

R

R

D

S

A

V

V

L

P

P

A

V

G

I

A

E

E

R

L

L

-

G

-

K

-

H

-

P

-

T

-

F

V

V

V

E

A

G

D
|
D

A
x
I

T

R

D

N

P

E

T

A

A

L

V

M

G

T

P

E

V

I

V

L

G

H

D

D

G

H

R

A

F

I

D

D

A

T

C

V

I

I

H

H

F
|
F

A
|
A

A
x
G

L

L

I
x
K

E

A

A

V

G

G

E

E

S

S

M

V

R

Q

H

K

P

P

E

L

R

E

F

Y

T

D

V

N

N

N

T

V

A

N

G

G

T

T

A

L

R

R

L

L

L

I

E

S

V

A

L

M

I

R

D

A

H

A

A

N

V

V

P

K

R

N

F

F

V

I

L

F

S
|
S

S

S

T
x
S

A
|
A

A
x
T

V

V

Y

Y

G

G

E

D

P

N

E

P

R

K

T

I

P

P

I

Y

D

V

E

E

D

S

H

F

P

P

L

T

A

G

-

T

P

P

T

Q

N

S

A

P

Y
|
Y

G

G

E

K

S

S

K
|
K

L

L

L

M

I

V

E

E

R

Q

M

I

L

L

A

T

W

D

H

L

H

Q

R

K

I

A

H

Q

-

P

G

D

L

W

R

S

T

I

A

A

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

A
x
P

A

V

G

G

A

A

T

H

P

P

G

S

R

G

G

D

E
x
M

R

G

H

E

D

D

P

P

E

Q

-

G

-

I

-

P

T

N

H
x
N

L
|
L

I

M

P

P

L

Y

V

I

L

A

Q

Q

V

V

A

A

A

V

G

G

E

R

R

R

D

D

Q

S

V

L

S
x
A

I
|
I

F
|
F

G

G

S

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

A

G

I

V

R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

A

M

D

D

L

L

A

A

D

D

A

G

H

H

V

V

R

V

A

A

V

M

E

E

R

K

L

L

-

A

D

N

Q

K

L

P

G

G

T

V

C

H

T

I

V

Y

N

N

L

L

G

G

S

A

G

G

T

V

G

G

F

N

S

S

V

V

R

L

E

D

V

V

I

V

D

N

A

A

A

F

R

S

R

K

V

A

T

C

G

G

H

K

A

P

I

V

P

N

V

Y

I

H

E

F

A

A

D

P

R

R

R
|
R

A

E

G

G

D
|
D

P

L

A

P

R

A

L
x
Y

V

W

A

A

S

D

A

A

T

S

R

K

A

A

R

D

E

R

L

E

L

L

G

N

W

W

E

R

P

V

A

T

I

R

T

T

D

-

L

L

D

D

R

E

I

M

V

A

A

Q

D

D

A

T

W

W

N

H

A

W

T

Q

V

S

D

R

H

H

A

P

G

Q

A

G

H

Y

P

P

active site: S124 (≠ T117), A125 (= A118), T126 (≠ A119), Y149 (= Y141), K153 (= K145), M189 (≠ E180)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (= I12), D31 (= D31), N32 (≠ D32), L33 (= L33), C34 (≠ S34), N35 (≠ T35), S36 (≠ G36), D58 (= D51), I59 (≠ A52), F80 (= F73), A81 (= A74), G82 (≠ A75), K84 (≠ I77), S122 (= S115), Y149 (= Y141), K153 (= K145), Y177 (= Y168), P180 (≠ A171)
binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: T126 (≠ A119), N179 (= N170), N199 (≠ H187), L200 (= L188), A216 (≠ S204), I217 (= I205), F218 (= F206), R231 (= R219), Y233 (= Y221), R292 (= R279), D295 (= D282), Y299 (≠ L286)

1naiA Udp-galactose 4-epimerase from escherichia coli, oxidized (see paper)
39% identity, 100% coverage: 1:325/325 of query aligns to 1:337/338 of 1naiA

query
sites
1naiA

M

M

R

R

V

V

L

L

V

V

T

T

G
|
G

G

G

A

S

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

T

T

C

A

V

A

Q

L

L

G

Q

A

N

G

G

H

H

E

D

V

V

T

I

V

I

L

L

D
|
D

D
x
N

L
|
L

S
x
C

T
x
N

G
x
S

H

K

R

R

D

S

A

V

V

L

P

P

A

V

G

I

A

E

E

R

L

L

-

G

-

K

-

H

-

P

-

T

-

F

V

V

V

E

A

G

D
|
D

A
x
I

T

R

D

N

P

E

T

A

A

L

V

M

G

T

P

E

V

I

V

L

G

H

D

D

G

H

R

A

F

I

D

D

A

T

C

V

I

I

H

H

F
|
F

A
|
A

A
x
G

L

L

I
x
K

E

A

A

V

G

G

E

E

S

S

M

V

R

Q

H

K

P

P

E

L

R

E

F

Y

T

D

V

N

N

N

T

V

A

N

G

G

T

T

A

L

R

R

L

L

L

I

E

S

V

A

L

M

I

R

D

A

H

A

A

N

V

V

P

K

R

N

F

F

V

I

L

F

S

S

T
x
S

A
|
A

A
x
T

V

V

Y

Y

G

G

E

D

P

N

E

P

R

K

T

I

P

P

I

Y

D

V

E

E

D

S

H

F

P

P

L

T

A

G

-

T

P

P

T

Q

N

S

A

P

Y
|
Y

G

G

E

K

S

S

K
|
K

L

L

L

M

I

V

E

E

R

Q

M

I

L

L

A

T

W

D

H

L

H

Q

R

K

I

A

H

Q

-

P

G

D

L

W

R

S

T

I

A

A

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

A
x
P

A

V

G

G

A

A

T

H

P

P

G

S

R

G

G

D

E
x
M

R

G

H
x
E

D

D

P
|
P

E

Q

-

G

-

I

-

P

T

N

H
x
N

L
|
L

I

M

P

P

L

Y

V

I

L

A

Q

Q

V

V

A

A

A

V

G

G

E

R

R

R

D

D

Q

S

V
x
L

S
x
A

I

I

F

F

G

G

S

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

A

G

I

V

R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

A

M

D

D

L

L

A

A

D

D

A

G

H

H

V

V

R

V

A

A

V

M

E

E

R

K

L

L

-

A

D

N

Q

K

L

P

G

G

T

V

C

H

T

I

V

Y

N

N

L

L

G

G

S

A

G

G

T

V

G

G

F

N

S

S

V

V

R

L

E

D

V

V

I

V

D

N

A

A

A

F

R

S

R

K

V

A

T

C

G

G

H

K

A

P

I

V

P

N

V

Y

I

H

E

F

A

A

D

P

R

R

R
|
R

A

E

G

G

D

D

P

L

A

P

R

A

L

Y

V

W

A

A

S

D

A

A

T

S

R

K

A

A

R

D

E

R

L

E

L

L

G

N

W

W

E

R

P

V

A

T

I

R

T

T

D

-

L

L

D

D

R

E

I

M

V

A

A

Q

D

D

A

T

W

W

N

H

A

W

T

Q

V

S

D

R

H

H

A

P

G

Q

A

G

H

Y

P

P

active site: S124 (≠ T117), A125 (= A118), T126 (≠ A119), Y149 (= Y141), K153 (= K145), M189 (≠ E180)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (= I12), D31 (= D31), N32 (≠ D32), L33 (= L33), C34 (≠ S34), N35 (≠ T35), S36 (≠ G36), D58 (= D51), I59 (≠ A52), F80 (= F73), A81 (= A74), G82 (≠ A75), K84 (≠ I77), Y149 (= Y141), K153 (= K145), Y177 (= Y168), P180 (≠ A171)
binding 1,3-propandiol: N35 (≠ T35), K84 (≠ I77), E191 (≠ H182), P193 (= P184)
binding uridine-5'-diphosphate: N179 (= N170), N199 (≠ H187), L200 (= L188), L215 (≠ V203), A216 (≠ S204), R231 (= R219), Y233 (= Y221), R292 (= R279)

1lrjA Crystal structure of e. Coli udp-galactose 4-epimerase complexed with udp-n-acetylglucosamine (see paper)
39% identity, 100% coverage: 1:325/325 of query aligns to 1:337/338 of 1lrjA

query
sites
1lrjA

M

M

R

R

V

V

L

L

V

V

T

T

G
|
G

G

G

A

S

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

T

T

C

A

V

A

Q

L

L

G

Q

A

N

G

G

H

H

E

D

V

V

T

I

V

I

L

L

D
|
D

D
x
N

L
|
L

S
x
C

T
x
N

G
x
S

H

K

R

R

D

S

A

V

V

L

P

P

A

V

G

I

A

E

E

R

L

L

-

G

-

K

-

H

-

P

-

T

-

F

V

V

V

E

A

G

D
|
D

A
x
I

T

R

D

N

P

E

T

A

A

L

V

M

G

T

P

E

V

I

V

L

G

H

D

D

G

H

R

A

F

I

D

D

A

T

C

V

I

I

H

H

F
|
F

A
|
A

A
x
G

L

L

I
x
K

E

A

A
x
V

G

G

E

E

S

S

M

V

R

Q

H

K

P

P

E

L

R

E

F

Y

T

D

V

N

N

N

T

V

A

N

G

G

T

T

A

L

R

R

L

L

L

I

E

S

V

A

L

M

I

R

D

A

H

A

A

N

V

V

P

K

R

N

F

F

V

I

L

F

S

S

T
x
S

A
|
A

A
x
T

V

V

Y

Y

G

G

E

D

P

N

E

P

R

K

T

I

P

P

I

Y

D

V

E

E

D

S

H

F

P

P

L

T

A

G

-

T

P

P

T

Q

N

S

A

P

Y
|
Y

G

G

E

K

S

S

K
|
K

L

L

L

M

I

V

E

E

R

Q

M

I

L

L

A

T

W

D

H

L

H

Q

R

K

I

A

H

Q

-

P

G

D

L

W

R

S

T

I

A

A

A

L

L

L

R

R

Y
|
Y

F
|
F

N
|
N

A
x
P

A

V

G

G

A

A

T

H

P

P

G

S

R

G

G

D

E
x
M

R

G

H

E

D

D

P

P

E

Q

-

G

-

I

-

P

T

N

H

N

L
|
L

I

M

P

P

L

Y

V

I

L

A

Q

Q

V

V

A

A

A

V

G

G

E

R

R

R

D

D

Q

S

V
x
L

S
x
A

I

I

F
|
F

G

G

S

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

A

G

I

V

R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

A

M

D

D

L

L

A

A

D

D

A

G

H

H

V

V

R

V

A

A

V

M

E

E

R

K

L

L

-

A

D

N

Q

K

L

P

G

G

T

V

C

H

T

I

V

Y

N

N

L

L

G

G

S

A

G

G

T

V

G

G

F

N

S

S

V
|
V

R

L

E

D

V

V

I

V

D

N

A

A

A

F

R

S

R

K

V

A

T

C

G

G

H

K

A

P

I

V

P

N

V

Y

I

H

E

F

A

A

D

P

R

R

R
|
R

A

E

G

G

D
|
D

P

L

A

P

R

A

L

Y

V

W

A

A

S

D

A

A

T

S

R

K

A

A

R

D

E

R

L

E

L

L

G

N

W

W

E

R

P

V

A

T

I

R

T

T

D

-

L

L

D

D

R

E

I

M

V

A

A

Q

D

D

A

T

W

W

N

H

A

W

T

Q

V

S

D

R

H

H

A

P

G

Q

A

G

H

Y

P

P

active site: S124 (≠ T117), A125 (= A118), T126 (≠ A119), Y149 (= Y141), K153 (= K145), M189 (≠ E180)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (= I12), D31 (= D31), N32 (≠ D32), L33 (= L33), C34 (≠ S34), N35 (≠ T35), S36 (≠ G36), D58 (= D51), I59 (≠ A52), F80 (= F73), A81 (= A74), G82 (≠ A75), K84 (≠ I77), Y149 (= Y141), K153 (= K145), Y177 (= Y168), P180 (≠ A171)
binding uridine-diphosphate-n-acetylglucosamine: V86 (≠ A79), S124 (≠ T117), F178 (= F169), N179 (= N170), L200 (= L188), L215 (≠ V203), A216 (≠ S204), F218 (= F206), R231 (= R219), Y233 (= Y221), V269 (= V256), R292 (= R279), D295 (= D282)

1kvrA Udp-galactose 4-epimerase complexed with udp-phenol (see paper)
39% identity, 100% coverage: 1:325/325 of query aligns to 1:337/338 of 1kvrA

query
sites
1kvrA

M

M

R

R

V

V

L

L

V

V

T

T

G
|
G

G

G

A

S

G
|
G

F
x
Y

I
|
I

G

G

A

S

T

H

T

T

T

C

A

V

A

Q

L

L

G

Q

A

N

G

G

H

H

E

D

V

V

T

I

V

I

L

L

D
|
D

D
x
N

L

L

S
x
C

T
x
N

G
x
S

H

K

R

R

D

S

A

V

V

L

P

P

A

V

G

I

A

E

E

R

L

L

-

G

-

K

-

H

-

P

-

T

-

F

V

V

V

E

A

G

D
|
D

A
x
I

T

R

D

N

P

E

T

A

A

L

V

M

G

T

P

E

V

I

V

L

G

H

D

D

G

H

R

A

F

I

D

D

A

T

C

V

I

I

H

H

F
|
F

A
|
A

A
x
G

L

L

I
x
K

E

A

A

V

G

G

E

E

S

S

M

V

R

Q

H

K

P

P

E

L

R

E

F

Y

T

D

V

N

N

N

T

V

A

N

G

G

T

T

A

L

R

R

L

L

L

I

E

S

V

A

L

M

I

R

D

A

H

A

A

N

V

V

P

K

R

N

F

F

V

I

L

F

S
|
S

T
x
A

A
|
A

A
x
T

V

V

Y

Y

G

G

E

D

P

Q

E

P

R

K

T

I

P

P

I

Y

D

V

E

E

D

S

H

F

P

P

L

T

A

G

-

T

P

P

T

Q

N

S

A

P

Y
|
Y

G

G

E

K

S

S

K
|
K

L

L

L

M

I

V

E

E

R

Q

M

I

L

L

A

T

W

D

H

L

H

Q

R

K

I

A

H

Q

-

P

G

D

L

W

R

S

T

I

A

A

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

A
x
P

A

V

G

G

A

A

T

H

P

P

G

S

R

G

G

D

E
x
M

R

G

H

E

D

D

P

P

E

Q

-

G

-

I

-

P

T
x
N

H
x
N

L
|
L

I

M

P

P

L

Y

V

I

L

A

Q

Q

V

V

A

A

A

V

G

G

E

R

R

R

D

D

Q

S

V

L

S
x
A

I
|
I

F
|
F

G

G

S

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

A

G

I

V

R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

A

M

D

D

L

L

A

A

D

D

A

G

H

H

V

V

R

V

A

A

V

M

E

E

R

K

L

L

-

A

D

N

Q

K

L

P

G

G

T

V

C

H

T

I

V

Y

N

N

L

L

G

G

S

A

G

G

T

V

G

G

F

N

S

S

V
|
V

R

L

E

D

V

V

I

V

D

N

A

A

A

F

R

S

R

K

V

A

T

C

G

G

H

K

A

P

I

V

P

N

V

Y

I

H

E

F

A

A

D

P

R

R

R
|
R

A

E

G

G

D
|
D

P

L

A

P

R

A

L

Y

V

W

A

A

S

D

A

A

T

S

R

K

A

A

R

D

E

R

L

E

L

L

G

N

W

W

E

R

P

V

A

T

I

R

T

T

D

-

L

L

D

D

R

E

I

M

V

A

A

Q

D

D

A

T

W

W

N

H

A

W

T

Q

V

S

D

R

H

H

A

P

G

Q

A

G

H

Y

P

P

active site: A124 (≠ T117), A125 (= A118), T126 (≠ A119), Y149 (= Y141), K153 (= K145), M189 (≠ E180)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (= I12), D31 (= D31), N32 (≠ D32), C34 (≠ S34), N35 (≠ T35), S36 (≠ G36), D58 (= D51), I59 (≠ A52), F80 (= F73), A81 (= A74), G82 (≠ A75), K84 (≠ I77), S122 (= S115), S123 (= S116), Y149 (= Y141), K153 (= K145), Y177 (= Y168), P180 (≠ A171)
binding uridine-5'-diphosphate: N179 (= N170), N198 (≠ T186), N199 (≠ H187), L200 (= L188), A216 (≠ S204), I217 (= I205), F218 (= F206), R231 (= R219), Y233 (= Y221), V269 (= V256), R292 (= R279), D295 (= D282)

Query Sequence

>WP_052665615.1 NCBI__GCF_000969705.1:WP_052665615.1
MRVLVTGGAGFIGATTTAALLGAGHEVTVLDDLSTGHRDAVPAGAELVVADATDPTAVGP
VVGDGRFDACIHFAALIEAGESMRHPERFFTVNTAGTARLLEVLIDHAVPRFVLSSTAAV
YGEPERTPIDEDHPLAPTNAYGESKLLIERMLAWHHRIHGLRTAALRYFNAAGATPGRGE
RHDPETHLIPLVLQVAAGERDQVSIFGSDYPTPDGTAIRDYVHVADLADAHVRAVERLDQ
LGTCTVNLGSGTGFSVREVIDAARRVTGHAIPVIEADRRAGDPARLVASATRARELLGWE
PAITDLDRIVADAWNATVDHAGAHP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory