SitesBLAST
Comparing WP_052666358.1 NCBI__GCF_000969705.1:WP_052666358.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5mh6A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-ketohexanoic acid and NAD+ (1.35 a resolution)
37% identity, 72% coverage: 81:297/303 of query aligns to 83:305/306 of 5mh6A
- binding 2-Ketohexanoic acid: H89 (≠ Y87), R224 (= R216), H272 (= H264), Y280 (≠ G272)
- binding magnesium ion: T130 (vs. gap), A132 (≠ L124), F210 (≠ I202), E211 (≠ A203), M213 (≠ L205), G225 (= G217), P226 (≠ R218), V228 (≠ I220), E230 (≠ T222), D241 (≠ R233), S251 (≠ D243)
- binding nicotinamide-adenine-dinucleotide: T86 (≠ K84), H89 (≠ Y87), G142 (= G134), T143 (≠ E135), L144 (≠ I136), R164 (= R156), P196 (≠ A188), T201 (= T193), V222 (= V214), A223 (= A215), R224 (= R216), H272 (= H264), S274 (≠ G266)
Sites not aligning to the query:
5mhaB D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
37% identity, 72% coverage: 81:297/303 of query aligns to 85:307/308 of 5mhaB
- binding 2-Ketohexanoic acid: R226 (= R216), H274 (= H264), Y282 (≠ G272)
- binding (2R)-2-hydroxyhexanoic acid: H91 (≠ Y87), Y282 (≠ G272)
- binding magnesium ion: F212 (≠ I202), E213 (≠ A203), M215 (≠ L205), D243 (≠ R233)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T88 (≠ K84), L143 (≠ G133), G144 (= G134), T145 (≠ E135), L146 (≠ I136), R165 (= R155), R166 (= R156), S167 (≠ H157), P180 (≠ S170), T197 (≠ L187), P198 (≠ A188), T203 (= T193), V224 (= V214), A225 (= A215), R226 (= R216), H274 (= H264), S276 (≠ G266)
Sites not aligning to the query:
5mhaA D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
37% identity, 72% coverage: 81:297/303 of query aligns to 85:307/308 of 5mhaA
- binding 2-Ketohexanoic acid: H91 (≠ Y87), R226 (= R216), H274 (= H264), Y282 (≠ G272)
- binding magnesium ion: T132 (vs. gap), A134 (≠ L124)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T88 (≠ K84), L143 (≠ G133), G144 (= G134), T145 (≠ E135), L146 (≠ I136), R165 (= R155), R166 (= R156), S167 (≠ H157), P180 (≠ S170), T197 (≠ L187), P198 (≠ A188), T203 (= T193), V224 (= V214), A225 (= A215), R226 (= R216), H274 (= H264), S276 (≠ G266)
Sites not aligning to the query:
5mh5A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-keto-hexanoic acid and NADP+ (1.4 a resolution)
37% identity, 72% coverage: 81:297/303 of query aligns to 85:307/308 of 5mh5A
- binding 2-Ketohexanoic acid: H91 (≠ Y87), R226 (= R216), H274 (= H264), Y282 (≠ G272)
- binding magnesium ion: T132 (vs. gap), A134 (≠ L124), F212 (≠ I202), E213 (≠ A203), M215 (≠ L205), D243 (≠ R233)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: T88 (≠ K84), G142 (= G132), L143 (≠ G133), G144 (= G134), T145 (≠ E135), L146 (≠ I136), R165 (= R155), R166 (= R156), S167 (≠ H157), T197 (≠ L187), P198 (≠ A188), T203 (= T193), V224 (= V214), A225 (= A215), R226 (= R216), H274 (= H264), S276 (≠ G266)
Sites not aligning to the query:
3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
33% identity, 82% coverage: 50:297/303 of query aligns to 59:310/311 of 3bazA
- active site: L98 (≠ Y87), R230 (= R216), A251 (= A237), D254 (= D240), E259 (= E245), H277 (= H264)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (≠ A65), G149 (= G132), L150 (≠ G133), G151 (= G134), R152 (≠ E135), I153 (= I136), S172 (≠ R155), R173 (= R156), S174 (≠ H157), C201 (≠ L187), P202 (≠ A188), T207 (= T193), I228 (≠ V214), G229 (≠ A215), R230 (= R216), D254 (= D240), H277 (= H264), G279 (= G266)
Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
33% identity, 82% coverage: 50:297/303 of query aligns to 61:312/313 of Q65CJ7
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
33% identity, 68% coverage: 86:291/303 of query aligns to 99:315/334 of 5aovA
- active site: L100 (≠ Y87), R241 (= R216), D265 (= D240), E270 (= E245), H288 (= H264)
- binding glyoxylic acid: R241 (= R216), H288 (= H264)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: T104 (≠ V91), F158 (≠ G133), G159 (= G134), R160 (≠ E135), I161 (= I136), S180 (≠ R155), R181 (= R156), A211 (= A186), V212 (≠ L187), P213 (≠ A188), T218 (= T193), I239 (≠ V214), A240 (= A215), R241 (= R216), H288 (= H264), G290 (= G266)
Sites not aligning to the query:
Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
35% identity, 57% coverage: 120:293/303 of query aligns to 148:322/328 of Q9UBQ7
- GRI 162:164 (≠ GEI 134:136) binding NADP(+)
- RQPR 185:188 (≠ R--R 155:156) binding NADP(+)
- S217 (≠ A188) binding NADP(+)
- I243 (≠ V214) binding NADP(+)
- R245 (= R216) binding substrate
- D269 (= D240) binding substrate
- HIGS 293:296 (≠ HVGN 264:267) binding substrate
- G295 (= G266) binding NADP(+)
Sites not aligning to the query:
2gcgA Ternary crystal structure of human glyoxylate reductase/hydroxypyruvate reductase (see paper)
35% identity, 57% coverage: 120:293/303 of query aligns to 144:318/324 of 2gcgA
- active site: R241 (= R216), D265 (= D240), E270 (= E245), H289 (= H264)
- binding (2r)-2,3-dihydroxypropanoic acid: R241 (= R216), H289 (= H264)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G156 (= G132), G158 (= G134), I160 (= I136), G180 (≠ V154), R181 (= R155), R184 (= R156), C212 (≠ L187), S213 (≠ A188), T218 (= T193), I239 (≠ V214), R241 (= R216), D265 (= D240), H289 (= H264), G291 (= G266)
Sites not aligning to the query:
8oq2A Binding of NADP to a formate dehydrogenase from starkeya novella.
32% identity, 72% coverage: 77:293/303 of query aligns to 135:362/381 of 8oq2A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: N147 (≠ E89), V151 (= V91), G201 (= G134), R202 (≠ E135), I203 (= I136), Q222 (≠ R155), R223 (= R156), H224 (= H157), V256 (≠ L187), P257 (≠ A188), H259 (≠ T190), T262 (= T193), T283 (≠ V214), A284 (= A215), R285 (= R216), D309 (= D240), H333 (= H264), S335 (≠ G266), G336 (≠ N267)
Sites not aligning to the query:
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
36% identity, 57% coverage: 120:291/303 of query aligns to 144:314/332 of 6biiA
- active site: R240 (= R216), D264 (= D240), E269 (= E245), H287 (= H264)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G156 (= G132), F157 (≠ G133), G158 (= G134), R159 (≠ E135), I160 (= I136), A179 (≠ R155), R180 (= R156), S181 (≠ H157), K183 (≠ D159), V211 (≠ L187), P212 (≠ A188), E216 (= E192), T217 (= T193), V238 (= V214), A239 (= A215), R240 (= R216), D264 (= D240), H287 (= H264), G289 (= G266)
Sites not aligning to the query:
3wr5A Structural basis on the efficient co2 reduction of acidophilic formate dehydrogenase
32% identity, 72% coverage: 77:293/303 of query aligns to 139:366/401 of 3wr5A
- active site: N151 (≠ E89), R289 (= R216), D313 (= D240), Q318 (≠ E245), H337 (= H264)
- binding nicotinamide-adenine-dinucleotide: N151 (≠ E89), V155 (= V91), A203 (≠ G132), G205 (= G134), R206 (≠ E135), I207 (= I136), D226 (≠ R155), R227 (= R156), V260 (≠ L187), P261 (≠ A188), T287 (≠ V214), A288 (= A215), R289 (= R216), D313 (= D240), H337 (= H264), S339 (≠ G266), G340 (≠ N267)
Sites not aligning to the query:
2gsdA NAD-dependent formate dehydrogenase from bacterium moraxella sp.C2 in complex with NAD and azide (see paper)
32% identity, 68% coverage: 88:293/303 of query aligns to 147:361/399 of 2gsdA
- active site: R284 (= R216), D308 (= D240), Q313 (≠ E245), H332 (= H264)
- binding azide ion: R284 (= R216), H332 (= H264)
- binding nicotinamide-adenine-dinucleotide: V150 (= V91), A198 (≠ G132), G200 (= G134), R201 (≠ E135), I202 (= I136), D221 (≠ R155), R222 (= R156), P256 (≠ A188), H258 (≠ T190), T261 (= T193), T282 (≠ V214), A283 (= A215), R284 (= R216), D308 (= D240), H332 (= H264), S334 (≠ G266), G335 (≠ N267)
Sites not aligning to the query:
4zqbB Crystal structure of NADP-dependent dehydrogenase from rhodobactersphaeroides in complex with NADP and sulfate
32% identity, 90% coverage: 31:303/303 of query aligns to 46:316/316 of 4zqbB
- active site: L99 (vs. gap), R231 (= R216), E260 (= E245), H279 (= H264)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R93 (vs. gap), M103 (vs. gap), G147 (= G132), L148 (≠ G133), G149 (= G134), E150 (= E135), L151 (≠ I136), W169 (≠ V154), S170 (≠ R155), R171 (= R156), S172 (≠ H157), K174 (≠ D159), L202 (= L187), P203 (≠ A188), F229 (≠ V214), R231 (= R216), H279 (= H264), S281 (vs. gap), A282 (vs. gap), Y316 (= Y303)
2nadA High resolution structures of holo and apo formate dehydrogenase (see paper)
31% identity, 72% coverage: 77:293/303 of query aligns to 134:361/391 of 2nadA
- active site: N146 (≠ E89), R284 (= R216), D308 (= D240), Q313 (≠ E245), H332 (= H264)
- binding azide ion: N146 (≠ E89), R284 (= R216)
- binding nicotinamide-adenine-dinucleotide: N146 (≠ E89), V150 (= V91), A198 (≠ G132), G200 (= G134), R201 (≠ E135), I202 (= I136), D221 (≠ R155), P256 (≠ A188), E260 (= E192), T261 (= T193), T282 (≠ V214), A283 (= A215), R284 (= R216), D308 (= D240), H332 (= H264), S334 (≠ G266), G335 (≠ N267)
Sites not aligning to the query:
P33160 Formate dehydrogenase; FDH; NAD-dependent formate dehydrogenase; EC 1.17.1.9 from Pseudomonas sp. (strain 101) (Achromobacter parvulus T1) (see 3 papers)
31% identity, 72% coverage: 77:293/303 of query aligns to 135:362/401 of P33160
- N147 (≠ E89) binding substrate
- S148 (≠ P90) binding NAD(+)
- RI 202:203 (≠ EI 135:136) binding NAD(+)
- D222 (≠ R155) binding NAD(+)
- C256 (≠ L187) mutation C->S,M: High resistance to inactivation by Hg(2+), Increased stability at 25 degree Celsius and decreased thermostability at 45 degree Celsius.
- PLHPE 257:261 (≠ ALTPE 188:192) binding NAD(+)
- T283 (≠ V214) binding NAD(+)
- D309 (= D240) binding NAD(+)
- HISG 333:336 (≠ HVGN 264:267) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 123 binding substrate
- 381 binding NAD(+)
5vg6B Crystal structure of d-isomer specific 2-hydroxyacid dehydrogenase from xanthobacter autotrophicus py2 in complex with NADPH and mes.
31% identity, 86% coverage: 43:303/303 of query aligns to 43:315/315 of 5vg6B
- active site: M98 (≠ Y87), R230 (= R216), D254 (= D240), E259 (= E245), H278 (= H264)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A74 (= A65), R92 (≠ T81), M102 (≠ V91), L147 (≠ G133), G148 (= G134), D149 (≠ E135), L150 (≠ I136), W168 (≠ V154), S169 (≠ R155), R170 (= R156), T171 (≠ H157), K173 (≠ D159), L201 (= L187), P202 (≠ A188), T207 (= T193), V228 (= V214), R230 (= R216), H278 (= H264), A280 (≠ G266), S281 (≠ N267), Y315 (= Y303)
2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
40% identity, 39% coverage: 173:291/303 of query aligns to 198:315/333 of 2dbqA
- active site: R241 (= R216), D265 (= D240), E270 (= E245), H288 (= H264)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: A211 (= A186), V212 (≠ L187), P213 (≠ A188), T218 (= T193), I239 (≠ V214), A240 (= A215), R241 (= R216), D265 (= D240), H288 (= H264), G290 (= G266)
Sites not aligning to the query:
O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
40% identity, 39% coverage: 173:291/303 of query aligns to 198:315/334 of O58320
Sites not aligning to the query:
- 158:161 binding NADP(+)
- 180:182 binding NADP(+)
7jqhA Structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with phosphate and NADP+
30% identity, 71% coverage: 89:303/303 of query aligns to 98:313/313 of 7jqhA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: M100 (≠ V91), A145 (vs. gap), G146 (vs. gap), V147 (vs. gap), L148 (vs. gap), W166 (≠ Y150), S167 (≠ R155), R168 (= R156), T169 (≠ H157), K171 (≠ D159), L199 (= L187), P200 (≠ A188), L226 (≠ V214), A227 (= A215), R228 (= R216), D252 (= D240), H276 (= H264), A279 (≠ N267), Y313 (= Y303)
Sites not aligning to the query:
Query Sequence
>WP_052666358.1 NCBI__GCF_000969705.1:WP_052666358.1
MRPRCVVLPTGRAPALEEAVGRGGGDLVDVGEADALVWVDPDDPEGLRSVLDANPHVCWV
QLPWAGIEPYRDVLDDDRRWTCGKGVYAEPVAEHVLALTLGLLRSVGHYSRQQSWTGPRG
RNLLGARVTILGGGEITRTLVRLLLPFGCYLTVVRRHADPIDGVAEVLASDRLHEALPTT
DVLVLALALTPETEHIVDAAAIAALPDHAVVVNVARGRHIDTDALTTALQEGRLGGAGLD
VTDPEPLPDDHPLWTAHNVVITPHVGNTPEMGRVLLAARVAENVARFGRGDPLLGPVDVD
LGY
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory