SitesBLAST
Comparing WP_052717928.1 NCBI__GCF_000967895.1:WP_052717928.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 9 hits to proteins with known functional sites (download)
Q9SS48 Glycerol-3-phosphate dehydrogenase SDP6, mitochondrial; Protein SUGAR-DEPENDENT 6; EC 1.1.5.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 95% coverage: 23:529/532 of query aligns to 71:617/629 of Q9SS48
- S86 (≠ A38) mutation to F: In spd6-2; loss of 90% of activity.
- E546 (≠ W458) mutation to G: In spd6-1; loss of 90% of activity.
2rgoA Structure of alpha-glycerophosphate oxidase from streptococcus sp.: A template for the mitochondrial alpha-glycerophosphate dehydrogenase (see paper)
32% identity, 95% coverage: 16:523/532 of query aligns to 9:546/557 of 2rgoA
- binding flavin-adenine dinucleotide: G25 (= G32), I28 (= I35), T29 (= T36), E48 (≠ D55), M49 (≠ R56), Q50 (= Q57), T56 (= T63), S57 (= S64), A197 (≠ V197), S232 (≠ T232), G233 (= G233), W235 (= W235), G257 (= G254), G344 (= G341), L345 (≠ V342), Y357 (≠ L351), K382 (= K375), I383 (≠ L376), T384 (= T377)
2rgoB Structure of alpha-glycerophosphate oxidase from streptococcus sp.: A template for the mitochondrial alpha-glycerophosphate dehydrogenase (see paper)
31% identity, 89% coverage: 48:523/532 of query aligns to 39:517/530 of 2rgoB
- binding flavin-adenine dinucleotide: E46 (≠ D55), M47 (≠ R56), Q48 (= Q57), T54 (= T63), S55 (= S64), S58 (= S67), T59 (≠ S68), A193 (≠ V197), S227 (≠ T232), G228 (= G233), W230 (= W235), L338 (≠ V342), R339 (= R343), Y350 (≠ N352), K374 (= K375), I375 (≠ L376), T376 (= T377)
Sites not aligning to the query:
3da1A X-ray structure of the glycerol-3-phosphate dehydrogenase from bacillus halodurans complexed with fad. Northeast structural genomics consortium target bhr167.
31% identity, 94% coverage: 20:518/532 of query aligns to 13:482/496 of 3da1A
- binding flavin-adenine dinucleotide: I24 (≠ A31), G27 (= G34), I28 (= I35), T29 (= T36), E48 (≠ D55), M49 (≠ R56), G55 (= G62), S57 (= S64), S60 (= S67), T61 (≠ S68), L63 (≠ M70), H65 (= H72), V151 (= V197), G187 (= G233), W189 (= W235), L193 (= L239), G210 (= G254), T250 (= T294), G296 (= G341), R298 (= R343), G319 (= G374), K320 (= K375), L321 (= L376)
2qcuB Crystal structure of glycerol-3-phosphate dehydrogenase from escherichia coli (see paper)
31% identity, 82% coverage: 49:485/532 of query aligns to 27:475/501 of 2qcuB
- binding flavin-adenine dinucleotide: E33 (≠ D55), A34 (≠ R56), C39 (≠ W61), T41 (= T63), S42 (= S64), S45 (= S67), S46 (= S68), L48 (≠ M70), H50 (= H72), A172 (≠ V197), T206 (= T232), W209 (= W235), G231 (= G254), R317 (= R343), G353 (= G374), K354 (= K375), L355 (= L376), T356 (= T377)
- binding phosphate ion: R54 (= R76), Y55 (= Y77), R317 (= R343), R332 (= R358)
Sites not aligning to the query:
2r46A Crystal structure of escherichia coli glycerol-3-phosphate dehydrogenase in complex with 2-phosphopyruvic acid. (see paper)
31% identity, 82% coverage: 49:485/532 of query aligns to 27:475/495 of 2r46A
- binding flavin-adenine dinucleotide: L32 (≠ V54), E33 (≠ D55), A34 (≠ R56), T41 (= T63), S42 (= S64), A44 (≠ R66), S45 (= S67), S46 (= S68), L48 (≠ M70), A172 (≠ V197), T206 (= T232), G231 (= G254), R317 (= R343), G353 (= G374), K354 (= K375), L355 (= L376), T356 (= T377)
- binding phosphoenolpyruvate: R54 (= R76), Y55 (= Y77), R254 (= R279), T270 (= T294), D272 (= D296), R317 (= R343), R332 (= R358)
- binding n-(tris(hydroxymethyl)methyl)-3-aminopropanesulfonic acid: Q157 (≠ R182), V455 (≠ A465), D456 (≠ K466), H457 (≠ N467), W459 (≠ A469)
Sites not aligning to the query:
2r45A Crystal structure of escherichia coli glycerol-3-phosphate dehydrogenase in complex with 2-phospho-d-glyceric acid (see paper)
31% identity, 82% coverage: 49:485/532 of query aligns to 27:475/495 of 2r45A
- binding 2-phosphoglyceric acid: R54 (= R76), Y55 (= Y77), R254 (= R279), I255 (≠ P280), T270 (= T294), R317 (= R343), R332 (= R358)
- binding flavin-adenine dinucleotide: E33 (≠ D55), A34 (≠ R56), C39 (≠ W61), A40 (≠ G62), T41 (= T63), S42 (= S64), A44 (≠ R66), S45 (= S67), S46 (= S68), L48 (≠ M70), A172 (≠ V197), T206 (= T232), G207 (= G233), W209 (= W235), G231 (= G254), R317 (= R343), G353 (= G374), K354 (= K375), L355 (= L376), T356 (= T377)
- binding phosphate ion: Q212 (= Q238), G277 (= G301), P279 (≠ N304), V310 (≠ I336), Y398 (≠ P412), R401 (= R415), R405 (≠ S419), R462 (≠ H472), D464 (= D474), W468 (≠ L478)
- binding n-(tris(hydroxymethyl)methyl)-3-aminopropanesulfonic acid: Q157 (≠ R182), V160 (≠ Q185), V455 (≠ A465), D456 (≠ K466), H457 (≠ N467), W459 (≠ A469)
Sites not aligning to the query:
Q9HUH4 Probable FAD-dependent oxidoreductase PA4991; EC 1.-.-.- from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
24% identity, 44% coverage: 27:259/532 of query aligns to 8:232/391 of Q9HUH4
Sites not aligning to the query:
S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
27% identity, 36% coverage: 160:353/532 of query aligns to 136:310/369 of S5FMM4
- S202 (≠ T232) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
Sites not aligning to the query:
- 51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
- 54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
- 81 K→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
- 332 I→V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
- 342 M→V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.
Query Sequence
>WP_052717928.1 NCBI__GCF_000967895.1:WP_052717928.1
MWQEAWQSGNRELLLNRLRENKQDEWDVVVAGGGITGAGIAREAARRGLSVLLVDRQDFA
WGTSSRSSKMVHGGLRYIAAGDIKTTMHSVSERERLMNEAPGLVDQMGYLMAHYKGGFPG
PFVFNSLLRIYDFFAGKKYRKFHKLKNFEYLSPLINEHELIGGTQFADAVTDDSRLVIRV
LREAQKDGAEVINYVAVDSLIKENGNVVGVHLKNAENDEIYSVKAKVVINATGAWADQLR
GEVSDEKNIRPARGSHLVVPSWRLPVAQAFTAMHPDDQRPIFIFPWEGRTIIGTTDLDNK
GIDNKEVAMTRAELEYLMRVANFQFPKAGLVEKDIISSWAGVRPLVSSGALNPSKEKRNH
SVWSDNGLVTVSGGKLTTFRLIALDVLAAAEPYLPNFNAKDFGADMFTKFKPTHRLFKSL
PGYLKKRLQGHYGMDSDRLLEQAHANELDVIPGASAMWAELRWSAKNEAVVHLDDLLLRR
TRIGLLVEQGGLIYENKIKAICCQELNWTEPQWQEELERYKSIWNKYYSIPA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory