SitesBLAST
Comparing WP_053544274.1 NCBI__GCF_001277995.1:WP_053544274.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
A0A0H3JRU9 Pyruvate carboxylase; EC 6.4.1.1 from Staphylococcus aureus (strain Mu50 / ATCC 700699) (see 2 papers)
46% identity, 99% coverage: 9:1139/1140 of query aligns to 1:1145/1150 of A0A0H3JRU9
- R21 (= R29) mutation to A: Complete loss of catalytic activity.
- K119 (= K127) binding ATP
- K161 (= K168) binding ATP
- H211 (= H218) binding ATP
- E278 (= E285) binding ATP
- K411 (≠ R415) mutation to A: Complete loss of catalytic activity.
- RDAHQ 541:545 (= RDAHQ 542:546) binding substrate
- D542 (= D543) binding Mn(2+)
- A580 (= A581) mutation to T: Complete loss of catalytic activity.
- R614 (= R615) mutation to A: Complete loss of catalytic activity.
- Y621 (= Y622) mutation to A: Complete loss of catalytic activity.
- K712 (= K713) binding Mn(2+)
- H741 (= H742) binding Mn(2+)
- H743 (= H744) binding Mn(2+)
- Q838 (= Q839) mutation to A: About 2.5-fold loss of catalytic activity.
- T876 (= T877) mutation to A: Complete loss of catalytic activity.
- S879 (= S880) mutation to A: About 2-fold loss of catalytic activity.
- K880 (= K881) mutation to T: Complete loss of catalytic activity.
7wtbB Cryo-em structure of human pyruvate carboxylase with acetyl-coa (see paper)
47% identity, 99% coverage: 13:1139/1140 of query aligns to 7:1146/1147 of 7wtbB
- binding acetyl coenzyme *a: F22 (= F28), T26 (≠ Y32), R46 (= R52), Q47 (≠ S53), K48 (≠ F54), A49 (= A55), D50 (≠ S56), R367 (= R372), R414 (= R418), E418 (= E422), R420 (≠ T424), R422 (≠ S426), A462 (= A466), Q463 (≠ D467), R465 (≠ E469), K1025 (≠ R1018)
- binding phosphoaminophosphonic acid-adenylate ester: K163 (= K168), G168 (= G173), G169 (= G174), M173 (= M178), F207 (≠ A212), I208 (≠ V213), P211 (= P216), H240 (= H245)
- binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: D582 (= D584), Q839 (= Q839), T877 (= T877), S880 (= S880), K881 (= K881)
7wtdC Cryo-em structure of human pyruvate carboxylase with acetyl-coa in the intermediate state 1 (see paper)
47% identity, 99% coverage: 13:1139/1140 of query aligns to 6:1145/1146 of 7wtdC
- binding adenosine-5'-triphosphate: K162 (= K168), G167 (= G173), G168 (= G174), F206 (≠ A212), Q236 (= Q242), H239 (= H245), E292 (= E298)
- binding coenzyme a: F21 (= F28), R22 (= R29), T25 (≠ Y32), R45 (= R52), Q46 (≠ S53), K47 (≠ F54), A48 (= A55), D49 (≠ S56), E50 (= E57), R366 (= R372), R413 (= R418), A416 (= A421), R419 (≠ T424), Q462 (≠ D467), R464 (≠ E469), A465 (≠ Q470), Q466 (≠ G471), K1024 (≠ R1018), R1053 (= R1048)
P11498 Pyruvate carboxylase, mitochondrial; Pyruvic carboxylase; PCB; EC 6.4.1.1 from Homo sapiens (Human) (see 6 papers)
47% identity, 99% coverage: 13:1139/1140 of query aligns to 38:1177/1178 of P11498