SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_053545600.1 NCBI__GCF_001277995.1:WP_053545600.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0AAH0 Phosphate import ATP-binding protein PstB; ABC phosphate transporter; Phosphate-transporting ATPase; EC 7.3.2.1 from Escherichia coli (strain K12) (see paper)
55% identity, 100% coverage: 2:257/257 of query aligns to 9:257/257 of P0AAH0

query
sites
P0AAH0

S

S

K

K

L

I

K

Q

L

V

N

R

D

N

V

L

N

N

I

F

Y

Y

G

G

D

K

F

F

H

H

A

A

V

L

Q

K

N

N

V

I

N

N

L

L

E

D

V

I

P

A

A

K

R

N

S

Q

V

V

T

T

A

A

F

F

I

I

G

G

P

P

S

S

G

G

C

C

G
|
G

K
|
K

S

S

T

T

V

L

L

L

R

R

S

T

I

F

N

N

R

K

M

M

H

F

E

E

V

L

T

Y

P

P

G

E

A

Q

Y

R

V

A

K

E

G

G

E

E

I

I

L

L

D

D

G

G

E

D

N

N

I

I

Y

L

G

T

S

N

K

S

V

Q

D

D

P

I

V

A

A

L

V

L

R

R

N

A

T

K

I

V

G

G

M

M

V

V

F

F

Q

Q

K

K

A

P

N

T

P

P

F

F

P

P

T

-

M

M

S

S

I

I

E

Y

D

D

N

N

V

I

V

A

A

F

G

G

L

V

K

R

L

L

S

F

G

E

E

K

K

L

N

S

K

R

K

A

R

D

L

M

K

D

E

E

V

R

A

V

E

Q

K

W

S

A

L

L

R

T

G

K

A

A

N

A

L

L

W

W

E

N

E

E

V

T

K

K

D

D

R

K

L

L

D

H

K

Q

P

S

G

G

G

Y

G

S

L

L

S

S

G

G

Q

Q

R

R

L

L

C

C

I

I

A

A

R

R

A

G

I

I

A

A

V

I

E

R

P

P

E

E

I

V

L

L

M

L

D

D

E

E

P

P

C

C

S

S

A

A

L

L

D

D

P

P

I

I

S

S

T

T

L

G

A

R

V

I

E

E

D

E

L

L

I

I

H

T

E

E

L

L

K

K

E

Q

E

D

F

Y

T

T

I

V

V

V

I

I

V

V

T

T

H

H

N

N

M

M

Q

Q

A

A

R

R

V

C

S

S

D

D

Q

H

T

T

A

A

F

F

Y

M

S

Y

L

L

E

-

A

-

T

-

G

-

R

-

P

-

G

G

R

E

L

L

V

I

E

E

I

F

G

S

P

N

T

T

K

D

I

L

F

F

E

T

S

K

P

P

D

A

Q

K

K

K

E

Q

T

T

E

E

D

D

Y

Y

I

I

S

T

G

G

R

R

F

Y

G

G

G48 (= G41) mutation to I: Loss of phosphate transport.
K49 (= K42) mutation to Q: Loss of phosphate transport.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
40% identity, 96% coverage: 6:253/257 of query aligns to 4:237/240 of 4ymuJ

query
sites
4ymuJ

L

V

N

N

D

D

V

V

N

Y

I

K

Y

N

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

V

L

Q

K

N

G

V

V

N

T

L

L

E

K

V

V

P

N

A

K

R

G

S

E

V

V

T

V

A

V

F

I

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

L

R

R

S

C

I

I

N

N

R

L

M

L

H

E

E

E

V

P

T

T

P

-

G

-

A

-

Y

-

V

-

K

K

G

G

E

E

I

V

L

F

L

I

D

D

G

G

E

V

N

K

I

I

Y

N

G

N

S

G

K

K

V

V

D

N

P

I

V

N

A

K

V

V

R

R

N

Q

T

K

I

V

G

G

M

M

V

V

F

F

Q

Q

K

H

A

F

N

N

P

L

F

F

P

P

T

H

M

L

S

T

-

A

I

I

E

E

D

N

N

I

V

T

V

L

A

A

G

P

L

V

K

K

L

V

S

K

G

-

E

K

K

M

N

N

K

K

R

E

L

A

K

E

E

E

V

L

A

A

E

V

K

D

S

L

L

L

R

A

G

K

A

V

N

G

L

L

W

L

E

-

V

-

K

-

D

D

R

K

L

K

D

D

K

Q

P

Y

G

P

G

I

G

K

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

L

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

E

Q

P

P

E

E

I

V

L

M

L

L

M

F

D

D

E
|
E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

L

K

A

E

V

V

E

L

D

N

L

V

I

M

H

K

E

Q

L

L

K

A

E

N

E

E

-

G

F

M

T

T

I

M

V

V

I

V

V

V

T

T

H
|
H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

D

D

Q

R

T

V

A

I

F

F

Y

M

S

D

L

-

E

-

A

-

T

-

G

-

R

-

P

D

G

G

R

V

L

I

V

V

E

E

I

E

G

G

P

T

T

P

K

E

I

I

F

F

E

Y

S

R

P

A

D

K

Q

N

K

E

E

R

T

T

E

R

D

E

Y

F

I

L

S

S

binding adenosine-5'-triphosphate: F11 (≠ Y13), V16 (≠ A18), S36 (= S38), G37 (= G39), S38 (≠ C40), G39 (= G41), K40 (= K42), S41 (= S43), T42 (= T44), E162 (= E173), H194 (= H204)
binding magnesium ion: S41 (= S43), E162 (= E173)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
36% identity, 97% coverage: 4:253/257 of query aligns to 3:237/241 of 4u00A

query
sites
4u00A

L

I

K

R

L

I

N

R

D

N

V

L

N

H

I

K

Y

W

Y
x
F

G

G

D

P

F

L

H

H

A
x
V

V

L

Q

K

N

G

V

I

N

H

L

L

E

E

V

V

P

A

A

P

R

G

S

E

V

K

T

L

A

V

F

I

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

I

R

R

S

T

I

I

N

N

R

R

M

L

H

E

E

D

V

-

T

-

P

-

G

-

A

-

Y

F

V

Q

K

E

G

G

E

E

I

V

L

V

D

D

G

G

E

L

N

S

I

V

Y

K

G

D

S

D

K

R

V

A

D

L

P

R

V

-

A

E

V

I

R

R

N

R

T

E

I

V

G

G

M

M

V

V

F

F

Q

Q

K

Q

A

F

N

N

P

L

F

F

P

P

T

H

M

M

S

T

I

V

E

L

D

E

N

N

V

V

V

T

A

-

G

-

L

-

K

-

L

L

S

A

G

P

E

M

K

R

N

V

K

R

R

W

L

P

K

R

E

E

V

K

A

A

E

E

K

K

S

-

L

-

R

K

G

A

A

L

N

E

L

L

W

L

E

E

E

R

V

V

K

G

-

I

-

L

D

D

R

Q

L

A

D

R

K

K

P

Y

G

P

G

A

G

Q

L

L

S

S

G

G

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

E

E

P

P

E

K

I

I

L

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

L

G

A

E

V

V

E

L

D

D

L

V

I

M

H

R

E

D

L

L

K

A

E

Q

-

G

E

G

F

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

A

D

D

Q

R

T

V

A

V

F

F

Y

M

S

D

L

-

E

-

A

-

T

-

G

-

R

-

P

G

G

G

R

Q

L

I

V

V

E

E

I

E

G

G

P

R

T

P

K

E

I

I

F

F

E

T

S

R

P

P

D

K

Q

E

K

E

E

R

T

T

E

R

D

S

Y

F

I

L

S

Q

binding adenosine-5'-diphosphate: F12 (≠ Y13), V17 (≠ A18), S37 (= S38), G38 (= G39), S39 (≠ C40), G40 (= G41), K41 (= K42), S42 (= S43), T43 (= T44)

3c4jA Abc protein artp in complex with atp-gamma-s
36% identity, 95% coverage: 13:256/257 of query aligns to 13:242/242 of 3c4jA

query
sites
3c4jA

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

V

L

Q

K

N

G

V

I

N

N

L

V

E

H

V

I

P

R

A

E

R

G

S

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

F

L

L

R

R

S

C

I

L

N

N

R

L

M

L

H

E

E

D

V

-

T

-

P

-

G

-

A

-

Y

F

V

D

K

E

G

G

E

E

I

I

L

I

D

D

G

G

E

I

N

N

I

L

Y

K

G

A

S

K

K

D

V

T

D

N

P

L

V

N

A

K

V

V

R

R

N

E

T

E

I

V

G

G

M

M

V

V

F

F

Q

Q

K

R

A

F

N

N

P

L

F

F

P

P

T

H

M

M

S

T

I

V

E

L

D

N

N

N

V

I

V

T

A

-

G

-

L

-

K

-

L

L

S

A

G

P

E

M

K

K

N

V

K

R

K

K

R

W

L

P

K

R

E

E

V

K

A

A

E

E

K

A

S

-

L

-

R

K

G

A

A

M

N

E

L

L

W

L

E

D

E

K

V

V

-

G

-

L

K

K

D

D

R

K

L

A

D

H

K

A

P

Y

G

P

G

D

G

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

E

E

P

P

E

K

I

I

L

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

L

G

A

E

V

V

E

L

D

S

L

V

I

M

H

K

E

Q

L

L

K

A

E

N

E

E

-

G

F

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

D

D

Q

R

T

V

A

L

F

F

Y

M

S

D

L

-

E

-

A

-

T

-

G

-

R

-

P

G

G

G

R

Y

L

I

V

I

E

E

I

E

G

G

P

K

T

P

K

E

K

D

I

L

F

F

E

D

S

R

P

P

D

Q

Q

H

K

E

E

R

T

T

E

K

D

A

Y

F

I

L

S

S

G

K

R

V

F

F

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y13), V18 (≠ A18), S38 (= S38), G39 (= G39), S40 (≠ C40), G41 (= G41), K42 (= K42), S43 (= S43), T44 (= T44)

3c41J Abc protein artp in complex with amp-pnp/mg2+
36% identity, 95% coverage: 13:256/257 of query aligns to 13:242/242 of 3c41J

query
sites
3c41J

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

V

L

Q

K

N

G

V

I

N

N

L

V

E

H

V

I

P

R

A

E

R

G

S

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

V

F

L

L

R

R

S

C

I

L

N

N

R

L

M

L

H

E

E

D

V

-

T

-

P

-

G

-

A

-

Y

F

V

D

K

E

G

G

E

E

I

I

L

I

D

D

G

G

E

I

N

N

I

L

Y

K

G

A

S

K

K

D

V

T

D

N

P

L

V

N

A

K

V

V

R

R

N

E

T

E

I

V

G

G

M

M

V

V

F

F

Q

Q

K

R

A

F

N

N

P

L

F

F

P

P

T

H

M

M

S

T

I

V

E

L

D

N

N

N

V

I

V

T

A

-

G

-

L

-

K

-

L

L

S

A

G

P

E

M

K

K

N

V

K

R

K

K

R

W

L

P

K

R

E

E

V

K

A

A

E

E

K

A

S

-

L

-

R

K

G

A

A

M

N

E

L

L

W

L

E

D

E

K

V

V

-

G

-

L

K

K

D

D

R

K

L

A

D

H

K

A

P

Y

G

P

G

D

G

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

E

E

P

P

E

K

I

I

L

M

L

L

M

F

D
|
D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

L

G

A

E

V

V

E

L

D

S

L

V

I

M

H

K

E

Q

L

L

K

A

E

N

E

E

-

G

F

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

D

D

Q

R

T

V

A

L

F

F

Y

M

S

D

L

-

E

-

A

-

T

-

G

-

R

-

P

G

G

G

R

Y

L

I

V

I

E

E

I

E

G

G

P

K

T

P

K

E

K

D

I

L

F

F

E

D

S

R

P

P

D

Q

Q

H

K

E

E

R

T

T

E

K

D

A

Y

F

I

L

S

S

G

K

R

V

F

F

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y13), V18 (≠ A18), S38 (= S38), G39 (= G39), G41 (= G41), K42 (= K42), S43 (= S43), T44 (= T44)
binding magnesium ion: S43 (= S43), D163 (= D172)

2olkA Abc protein artp in complex with adp-beta-s
36% identity, 95% coverage: 13:256/257 of query aligns to 13:242/242 of 2olkA

query
sites
2olkA

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

V

L

Q

K

N

G

V

I

N

N

L

V

E

H

V

I

P

R

A

E

R

G

S

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

F

L

L

R

R

S

C

I

L

N

N

R

L

M

L

H

E

E

D

V

-

T

-

P

-

G

-

A

-

Y

F

V

D

K

E

G

G

E

E

I

I

L

I

D

D

G

G

E

I

N

N

I

L

Y

K

G

A

S

K

K

D

V

T

D

N

P

L

V

N

A

K

V

V

R

R

N

E

T

E

I

V

G

G

M

M

V

V

F

F

Q

Q

K

R

A

F

N

N

P

L

F

F

P

P

T

H

M

M

S

T

I

V

E

L

D

N

N

N

V

I

V

T

A

-

G

-

L

-

K

-

L

L

S

A

G

P

E

M

K

K

N

V

K

R

K

K

R

W

L

P

K

R

E

E

V

K

A

A

E

E

K

A

S

-

L

-

R

K

G

A

A

M

N

E

L

L

W

L

E

D

E

K

V

V

-

G

-

L

K

K

D

D

R

K

L

A

D

H

K

A

P

Y

G

P

G

D

G

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

E

E

P

P

E

K

I

I

L

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

L

G

A

E

V

V

E

L

D

S

L

V

I

M

H

K

E

Q

L

L

K

A

E

N

E

E

-

G

F

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

D

D

Q

R

T

V

A

L

F

F

Y

M

S

D

L

-

E

-

A

-

T

-

G

-

R

-

P

G

G

G

R

Y

L

I

V

I

E

E

I

E

G

G

P

K

T

P

K

E

K

D

I

L

F

F

E

D

S

R

P

P

D

Q

Q

H

K

E

E

R

T

T

E

K

D

A

Y

F

I

L

S

S

G

K

R

V

F

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y13), V18 (≠ A18), S38 (= S38), G39 (= G39), S40 (≠ C40), G41 (= G41), K42 (= K42), S43 (= S43), T44 (= T44)

2oljA Abc protein artp in complex with adp/mg2+
36% identity, 95% coverage: 13:256/257 of query aligns to 13:242/242 of 2oljA

query
sites
2oljA

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

V

L

Q

K

N

G

V

I

N

N

L

V

E

H

V

I

P

R

A

E

R

G

S

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

F

L

L

R

R

S

C

I

L

N

N

R

L

M

L

H

E

E

D

V

-

T

-

P

-

G

-

A

-

Y

F

V

D

K

E

G

G

E

E

I

I

L

I

D

D

G

G

E

I

N

N

I

L

Y

K

G

A

S

K

K

D

V

T

D

N

P

L

V

N

A

K

V

V

R

R

N

E

T

E

I

V

G

G

M

M

V

V

F

F

Q

Q

K

R

A

F

N

N

P

L

F

F

P

P

T

H

M

M

S

T

I

V

E

L

D

N

N

N

V

I

V

T

A

-

G

-

L

-

K

-

L

L

S

A

G

P

E

M

K

K

N

V

K

R

K

K

R

W

L

P

K

R

E

E

V

K

A

A

E

E

K

A

S

-

L

-

R

K

G

A

A

M

N

E

L

L

W

L

E

D

E

K

V

V

-

G

-

L

K

K

D

D

R

K

L

A

D

H

K

A

P

Y

G

P

G

D

G

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

E

E

P

P

E

K

I

I

L

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

L

G

A

E

V

V

E

L

D

S

L

V

I

M

H

K

E

Q

L

L

K

A

E

N

E

E

-

G

F

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

D

D

Q

R

T

V

A

L

F

F

Y

M

S

D

L

-

E

-

A

-

T

-

G

-

R

-

P

G

G

G

R

Y

L

I

V

I

E

E

I

E

G

G

P

K

T

P

K

E

K

D

I

L

F

F

E

D

S

R

P

P

D

Q

Q

H

K

E

E

R

T

T

E

K

D

A

Y

F

I

L

S

S

G

K

R

V

F

F

binding adenosine-5'-diphosphate: F13 (≠ Y13), V18 (≠ A18), S38 (= S38), G39 (= G39), S40 (≠ C40), G41 (= G41), K42 (= K42), S43 (= S43), T44 (= T44)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
37% identity, 91% coverage: 19:252/257 of query aligns to 42:259/382 of 7ahhC

query
sites
7ahhC

V

V

Q

Y

N

D

V

T

N

N

L

F

E

E

V

I

P

N

A

E

R

G

S

E

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S

S

G
|
G

C

S

G
|
G

K
|
K

S

S

T

T

V

L

L

L

R

R

S

L

I

L

N

N

R

R

M

L

H

I

E

E

V

P

T

T

P

-

G

-

A

-

Y

-

V

-

K

S

G

G

E

K

I

I

L

F

L

I

D

D

G

N

E

Q

N

D

I

V

Y

A

G

T

-

L

S

N

K

K

V

E

D

D

P

L

V

L

A

Q

V

V

R

R

-

R

N

K

T

T

I

M

G

S

M

M

V

V

F

F

Q

Q

K

N

A

F

N

G

P

L

F

F

P

P

T

H

M

R

S

T

I

I

E

L

D

E

N

N

V

T

V

E

A

Y

G

G

L

L

K

E

L

V

S

Q

G

N

E

V

K

P

N

K

K

E

R

R

L

R

K

K

E

R

V

-

A

A

E

E

K

K

S

A

L

L

R

D

G

N

A

A

N

N

L

L

W

L

E

D

E

-

V

F

K

K

D

D

R

Q

L

Y

D

P

K

K

P

Q

G

-

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

I

L

A

A

V

N

E

D

P

P

E

E

I

I

L

L

M

M

D
|
D

E
|
E

P

A

C

F

S

S

A

A

L

L

D

D

P

P

I

L

S

I

T

R

L

R

A

E

V

M

E

Q

D

D

L

E

I

L

H

L

E

E

L

L

K

Q

E

A

E

K

F

F

-

Q

-

K

T

T

I

I

V

I

I

F

V

V

T

S

H

H

N

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

D

D

Q

R

T

I

A

A

F

I

Y

M

S

-

L

-

E

-

A

-

T

-

G

-

R

K

P

D

G

G

R

K

L

I

V

M

E

Q

I

I

G

G

P

T

T

G

K

E

I

I

F

L

E

T

S

N

P

P

D

-

Q

-

K

-

E

-

T

A

E

N

D

D

Y

Y

I

V

binding phosphoaminophosphonic acid-adenylate ester: G62 (= G39), G64 (= G41), K65 (= K42), D187 (= D172), E188 (= E173)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
37% identity, 91% coverage: 19:252/257 of query aligns to 42:259/382 of 7aheC

query
sites
7aheC

V

V

Q

Y

N

D

V

T

N

N

L

F

E

E

V

I

P

N

A

E

R

G

S

E

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S

S

G

G

C

S

G

G

K

K

S

S

T

T

V

L

L

L

R

R

S

L

I

L

N

N

R

R

M

L

H

I

E

E

V

P

T

T

P

-

G

-

A

-

Y

-

V

-

K

S

G

G

E

K

I

I

L

F

L

I

D

D

G

N

E

Q

N

D

I

V

Y

A

G

T

-

L

S

N

K

K

V

E

D

D

P

L

V

L

A

Q

V

V

R

R

-

R

N

K

T

T

I

M

G

S

M

M

V

V

F

F

Q

Q

K

N

A

F

N

G

P

L

F

F

P

P

T

H

M

R

S

T

I

I

E

L

D

E

N

N

V

T

V

E

A

Y

G

G

L

L

K

E

L

V

S

Q

G

N

E

V

K

P

N

K

K

E

R

R

L

R

K

K

E

R

V

-

A

A

E

E

K

K

S

A

L

L

R

D

G

N

A

A

N

N

L

L

W

L

E

D

E

-

V

F

K

K

D

D

R

Q

L

Y

D

P

K

K

P

Q

G

-

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

I

L

A

A

V

N

E

D

P

P

E

E

I

I

L

L

M

M

D

D

E

E

P

A

C

F

S

S

A

A

L

L

D

D

P

P

I

L

S

I

T

R

L

R

A

E

V

M

E

Q

D

D

L

E

I

L

H

L

E

E

L

L

K

Q

E

A

E

K

F

F

-

Q

-

K

T

T

I

I

V

I

I

F

V

V

T

S

H

H

N

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

D

D

Q

R

T

I

A

A

F

I

Y

M

S

-

L

-

E

-

A

-

T

-

G

-

R

K

P

D

G

G

R

K

L

I

V

M

E

Q

I

I

G

G

P

T

T

G

K

E

I

I

F

L

E

T

S

N

P

P

D

-

Q

-

K

-

E

-

T

A

E

N

D

D

Y

Y

I

V

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

7ahdC Opua (e190q) occluded (see paper)
37% identity, 91% coverage: 18:252/257 of query aligns to 41:259/260 of 7ahdC

query
sites
7ahdC

A

G

V

V

Q

Y

N

D

V

T

N

N

L

F

E

E

V

I

P

N

A

E

R

G

S

E

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

L

R

R

S

L

I

L

N

N

R

R

M

L

H

I

E

E

V

P

T

T

P

S

G

G

A

-

Y

-

V

-

K

-

G

-

E

K

I

I

L

F

L

I

D

D

G

N

E

Q

N

D

I

V

Y

A

G

T

-

L

S

N

K

K

V

E

D

D

P

L

V

L

A

Q

V

V

R

R

-

R

N

K

T

T

I

M

G

S

M

M

V

V

F

F

Q
|
Q

K

N

A

F

N

G

P

L

F

F

P

P

T

H

M

R

S

T

I

I

E

L

D

E

N

N

V

T

V

E

A

Y

G

G

L

L

K

E

L

V

S

Q

G

N

E

V

K

P

N

K

K

E

R

R

L

R

K

K

E

R

V

-

A

A

E

E

K

K

S

A

L

L

R

D

G

N

A

A

N

N

L

L

W

L

E

D

E

-

V

F

K

K

D

D

R

Q

L

Y

D

P

K
|
K

P
x
Q

G

-

L

L

S
|
S

G

G

G
|
G

Q
x
M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

I

L

A

A

V

N

E

D

P

P

E

E

I

I

L

L

M

M

D

D

E
x
Q

P

A

C

F

S

S

A

A

L

L

D

D

P

P

I

L

S

I

T

R

L

R

A

E

V

M

E

Q

D

D

L

E

I

L

H

L

E

E

L

L

K

Q

E

A

E

K

F

F

-

Q

-

K

T

T

I

I

V

I

I

F

V

V

T

S

H
|
H

N

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

D

D

Q

R

T

I

A

A

F

I

Y

M

S

-

L

-

E

-

A

-

T

-

G

-

R

K

P

D

G

G

R

K

L

I

V

M

E

Q

I

I

G

G

P

T

T

G

K

E

I

I

F

L

E

T

S

N

P

P

D

-

Q

-

K

-

E

-

T

A

E

N

D

D

Y

Y

I

V

binding adenosine-5'-triphosphate: S61 (= S38), G62 (= G39), G64 (= G41), K65 (= K42), S66 (= S43), T67 (= T44), Q111 (= Q91), K161 (= K143), Q162 (≠ P144), S164 (= S149), G166 (= G151), M167 (≠ Q152), Q188 (≠ E173), H221 (= H204)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 39

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
32% identity, 98% coverage: 1:252/257 of query aligns to 1:234/369 of P19566

query
sites
P19566

M

M

S

A

K

S

L

V

K

Q

L

L

N

R

D

N

V

V

N

T

I

K

Y

A

Y

W

G

G

D

D

F

V

H

V

A

V

V

S

Q

K

N

D

V

I

N

N

L

L

E

D

V

I

P

H

A

D

R

G

S

E

V

F

T

V

A

V

F

F

I

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

S

S

T

T

V

L

L

L

R

R

S

M

I

I

N

A

R

G

M

L

H

E

E

T

V

I

T

T

P

S

G

G

A

D

Y

L

V

F

K

I

G

G

E

E

I

T

L

R

L

M

D

N

G

-

E

-

N

-

I

-

Y

-

G

-

S

-

K

-

V

-

D

D

P

I

V

P

A

P

V

A

R

E

N

R

T

G

I

V

G

G

M

M

V

V

F

F

Q

Q

K

S

A

Y

N

A

P
x
L

F

Y

P

P

T

H

M

L

S

S

I

V

E

A

D

E

N

N

V

M

V

S

A

F

G

G

L

L

K

K

L

L

S

A

G

G

E

A

K

K

N

K

K

E

-

V

-

M

-

N

K

Q

R

R

L

V

K

N

E

Q

V

V

A

A

E

E

K

-

S

V

L

L

R

Q

G

L

A

A

N

H

L

L

W

L

E

E

E

R

V

-

K

-

D

-

R

-

L

-

D

-

K

K

P

P

G

-

G

K

G

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

L

V

C

A

I

I

A

G

R

R

A

T

I

L

A

V

V

A

E

E

P

P

E

R

I

V

L

F

L

L

M

L

D

D

E

E

P
|
P

C

L

S

S

A

N

L

L

D
|
D

P

A

I

A

S

L

T

R

L

V

A

Q

V

M

E

R

D

I

L

E

I

I

H

S

E

R

L

L

K

H

E

K

E

R

F

L

-

G

-

R

T

T

I

M

V

I

I

Y

V

V

T

T

H

H

N

D

M

Q

Q

V

Q

E

A

A

A

M

R

T

V

L

S

A

D

D

Q

K

T

I

A

V

F

V

Y

-

S

-

L

L

E

D

A

A

T

-

G

-

R

-

P

-

G

G

R

R

L

V

V

A

E

Q

I

V

G

G

P

K

T

P

K

L

K

E

I

L

F

Y

E

H

S

Y

P

P

D

A

Q

D

K

R

E

F

T

V

E

A

D

G

Y

F

I

I

L86 (≠ P95) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P174) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D179) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
33% identity, 92% coverage: 16:252/257 of query aligns to 18:241/343 of P30750

query
sites
P30750

F

I

H

Q

A

A

V

L

Q

N

N

N

V

V

N

S

L

L

E

H

V

V

P

P

A

A

R

G

S

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

I

R

R

S

C

I

V

N

N

R

L

M

L

H

E

E

R

V

P

T

T

P

-

G

-

A

-

Y

-

V

-

K

E

G

G

E

S

I

V

L

L

L

V

D

D

G

G

E

Q

N

E

I

L

Y

T

G

T

-

L

S

S

K

E

V

S

D

E

P

L

V

T

A

K

V

A

R

R

N

R

T

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

K

H

A

F

N

N

P

L

F

L

P

S

T

S

M

R

S

T

I

V

E

F

D

G

N

N

V

V

V

A

A

L

G

P

L

L

K

E

L

L

S

D

G

N

-

T

-

P

-

K

E

D

K

E

N

V

K

K

K

R

R

R

L

V

K

T

E

E

V

L

A

L

E

-

K

-

S

S

L

L

R

V

G

G

A

-

N

-

L

-

W

-

E

-

V

L

K

G

D

D

R

K

L

H

D

D

K

S

P

Y

G

P

G

S

G

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

S

E

N

P

P

E

K

I

V

L

L

M

C

D

D

E
|
E

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

S

T

T

T

L

R

A

S

V

I

E

L

D

E

L

L

I

L

H

K

E

D

L

I

K

N

E

R

-

L

-

G

F

L

T

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

Q

C

T

V

A

A

F

V

Y

I

S

S

L

-

E

-

A

-

T

-

G

-

R

-

P

N

G

G

R

E

L

L

V

I

E

E

I

Q

G

D

P

T

T

V

K

S

K

E

I

V

F

F

E

S

S

H

P

P

D

K

Q

T

K

P

E

L

T

A

E

Q

D

K

Y

F

I

I

40:46 (vs. 38:44, 86% identical) binding ATP
E166 (= E173) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
31% identity, 98% coverage: 1:252/257 of query aligns to 1:234/371 of P68187

query
sites
P68187

M

M

S

A

K

S

L

V

K

Q

L

L

N

Q

D

N

V

V

N

T

I

K

Y

A

Y

W

G

G

D

E

F

V

H

V

A

V

V

S

Q

K

N

D

V

I

N

N

L

L

E

D

V

I

P

H

A

E

R

G

S

E

V

F

T

V

A

V

F

F

I

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

S

S

T

T

V

L

L

L

R

R

S

M

I

I

N

A

R

G

M

L

H

E

E

T

V

I

T

T

P

S

G

G

A

D

Y

L

V

F

K

I

G

G

E

E

I

K

L

R

L

M

D

N

G

-

E

-

N

-

I

-

Y

-

G

-

S

-

K

-

V

-

D

D

P

T

V

P

A

P

V

A

R

E

N

R

T

G

I

V

G

G

M

M

V

V

F

F

Q

Q

K

S

A

Y

N
x
A

P

L

F

Y

P

P

T

H

M

L

S

S

I

V

E

A

D

E

N

N

V

M

V

S

A

F

G

G

L

L

K

K

L

L

S

A

G

G

E

A

K
|
K

N

K

K

E

-

V

-

I

-

N

K

Q

R

R

L
x
V

K

N

E

Q

V
|
V

A

A

E
|
E

K

-

S

V

L

L

R

Q

G

L

A
|
A

N

H

L

L

W

-

E

-

V

-

K

-

D

-

R

-

L

L

D

D

K

R

P

K

G

P

G

K

G

A

L

L

S

S

G

G

G
|
G

Q

Q

Q

R

Q

Q

R

R

L

V

C

A

I

I

A

G

R

R

A

T

I

L

A

V

V

A

E

E

P

P

E

S

I

V

L

F

L

L

M

L

D
|
D

E

E

P

P

C

L

S

S

A

N

L

L

D

D

P

A

I

A

S

L

T

R

L

V

A

Q

V

M

E

R

D

I

L

E

I

I

H

S

E

R

L

L

K

H

E

K

E

R

F

L

-

G

-

R

T

T

I

M

V

I

I

Y

V

V

T

T

H

H

N

D

M

Q

Q

V

Q

E

A

A

A

M

R

T

V

L

S

A

D

D

Q

K

T

I

A

V

F

V

Y

-

S

-

L

L

E

D

A

A

T

-

G

-

R

-

P

-

G

G

R

R

L

V

V

A

E

Q

I

V

G

G

P

K

T

P

K

L

K

E

I

L

F

Y

E

H

S

Y

P

P

D

A

Q

D

K
x
R

E

F

T

V

E

A

D

G

Y

F

I

I

A85 (≠ N94) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (= K115) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ L120) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (= V123) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E125) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (= A131) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G151) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D172) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ K246) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
33% identity, 92% coverage: 16:252/257 of query aligns to 19:242/344 of 6cvlD

query
sites
6cvlD

F
x
I

H

Q

A

A

V

L

Q

N

N

N

V

V

N

S

L

L

E

H

V

V

P

P

A

A

R

G

S

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

I

R

R

S

C

I

V

N

N

R

L

M

L

H

E

E

R

V

P

T

T

P

-

G

-

A

-

Y

-

V

-

K

E

G

G

E

S

I

V

L

L

L

V

D

D

G

G

E

Q

N

E

I

L

Y

T

G

T

-

L

S

S

K

E

V

S

D

E

P

L

V

T

A

K

V

A

R

R

N

R

T

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

K

H

A

F

N

N

P

L

F

L

P

S

T

S

M

R

S

T

I

V

E

F

D

G

N

N

V

V

V

A

A

L

G

P

L

L

K

E

L

L

S

D

G

N

-

T

-

P

-

K

E

D

K

E

N

V

K

K

K

R

R

R

L

V

K

T

E

E

V

L

A

L

E

-

K

-

S

S

L

L

R

V

G

G

A

-

N

-

L

-

W

-

E

-

V

L

K

G

D

D

R

K

L

H

D

D

K

S

P

Y

G

P

G

S

G
x
N

L

L

S
|
S

G

G

Q
|
Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

S

E

N

P

P

E

K

I

V

L

L

M

C

D

D

E

Q

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

S

T

T

T

L

R

A

S

V

I

E

L

D

E

L

L

I

L

H

K

E

D

L

I

K

N

E

R

-

L

-

G

F

L

T

T

I

I

V

L

I

L

V

I

T

T

H
|
H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

Q

C

T

V

A

A

F

V

Y

I

S

S

L

-

E

-

A

-

T

-

G

-

R

-

P

N

G

G

R

E

L

L

V

I

E

E

I

Q

G

D

P

T

T

V

K

S

K

E

I

V

F

F

E

S

S

H

P

P

D

K

Q

T

K

P

E

L

T

A

E

Q

D

K

Y

F

I

I

binding phosphothiophosphoric acid-adenylate ester: I19 (≠ F16), S41 (= S38), G42 (= G39), A43 (≠ C40), G44 (= G41), K45 (= K42), S46 (= S43), T47 (= T44), N141 (≠ G147), S143 (= S149), Q146 (= Q152), H200 (= H204)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
33% identity, 92% coverage: 16:252/257 of query aligns to 19:242/344 of 3tuzC

query
sites
3tuzC

F

I

H

Q

A

A

V

L

Q

N

N

N

V

V

N

S

L

L

E

H

V

V

P

P

A

A

R

G

S

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S

S

G
|
G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

I

R

R

S

C

I

V

N

N

R

L

M

L

H

E

E

R

V

P

T

T

P

-

G

-

A

-

Y

-

V

-

K

E

G

G

E

S

I

V

L

L

L

V

D

D

G

G

E

Q

N

E

I

L

Y

T

G

T

-

L

S

S

K

E

V

S

D

E

P

L

V

T

A

K

V

A

R

R

N

R

T

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

K

H

A

F

N

N

P

L

F

L

P

S

T

S

M

R

S

T

I

V

E

F

D

G

N

N

V

V

V

A

A

L

G

P

L

L

K

E

L

L

S

D

G

N

-

T

-

P

-

K

E

D

K

E

N

V

K

K

K

R

R

R

L

V

K

T

E

E

V

L

A

L

E

-

K

-

S

S

L

L

R

V

G

G

A

-

N

-

L

-

W

-

E

-

V

L

K

G

D

D

R

K

L

H

D

D

K

S

P

Y

G

P

G

S

G

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

S

E

N

P

P

E

K

I

V

L

L

M

C

D

D

E

Q

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

S

T

T

T

L

R

A

S

V

I

E

L

D

E

L

L

I

L

H

K

E

D

L

I

K

N

E

R

-

L

-

G

F

L

T

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

Q

C

T

V

A

A

F

V

Y

I

S

S

L

-

E

-

A

-

T

-

G

-

R

-

P

N

G

G

R

E

L

L

V

I

E

E

I

Q

G

D

P

T

T

V

K

S

K

E

I

V

F

F

E

S

S

H

P

P

D

K

Q

T

K

P

E

L

T

A

E

Q

D

K

Y

F

I

I

binding adenosine-5'-diphosphate: G42 (= G39), G44 (= G41), K45 (= K42), S46 (= S43), T47 (= T44)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
33% identity, 92% coverage: 16:252/257 of query aligns to 19:242/344 of 3tuiC

query
sites
3tuiC

F

I

H

Q

A

A

V

L

Q

N

N

N

V

V

N

S

L

L

E

H

V

V

P

P

A

A

R

G

S

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S

S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

I

R

R

S

C

I

V

N

N

R

L

M

L

H

E

E

R

V

P

T

T

P

-

G

-

A

-

Y

-

V

-

K

E

G

G

E

S

I

V

L

L

L

V

D

D

G

G

E

Q

N

E

I

L

Y

T

G

T

-

L

S

S

K

E

V

S

D

E

P

L

V

T

A

K

V

A

R

R

N

R

T

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

K

H

A

F

N

N

P

L

F

L

P

S

T

S

M

R

S

T

I

V

E

F

D

G

N

N

V

V

V

A

A

L

G

P

L

L

K

E

L

L

S

D

G

N

-

T

-

P

-

K

E

D

K

E

N

V

K

K

K

R

R

R

L

V

K

T

E

E

V

L

A

L

E

-

K

-

S

S

L

L

R

V

G

G

A

-

N

-

L

-

W

-

E

-

V

L

K

G

D

D

R

K

L

H

D

D

K

S

P

Y

G

P

G

S

G

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

S

E

N

P

P

E

K

I

V

L

L

M

C

D

D

E

Q

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

S

T

T

T

L

R

A

S

V

I

E

L

D

E

L

L

I

L

H

K

E

D

L

I

K

N

E

R

-

L

-

G

F

L

T

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

Q

C

T

V

A

A

F

V

Y

I

S

S

L

-

E

-

A

-

T

-

G

-

R

-

P

N

G

G

R

E

L

L

V

I

E

E

I

Q

G

D

P

T

T

V

K

S

K

E

I

V

F

F

E

S

S

H

P

P

D

K

Q

T

K

P

E

L

T

A

E

Q

D

K

Y

F

I

I

binding adenosine-5'-diphosphate: G42 (= G39), A43 (≠ C40), G44 (= G41), K45 (= K42), S46 (= S43), T47 (= T44)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
31% identity, 98% coverage: 2:252/257 of query aligns to 1:233/371 of 3puyA

query
sites
3puyA

S

A

K

S

L

V

K

Q

L

L

N

Q

D

N

V

V

N

T

I

K

Y

A

Y
x
W

G

G

D

E

F

V

H

V

A

V

V

S

Q

K

N

D

V

I

N

N

L

L

E

D

V

I

P

H

A

E

R

G

S

E

V

F

T

V

A

V

F

F

I

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

L

R

R

S

M

I

I

N

A

R

G

M

L

H

E

E

T

V

I

T

T

P

S

G

G

A

D

Y

L

V

F

K

I

G

G

E

E

I

K

L

R

L

M

D

N

G

-

E

-

N

-

I

-

Y

-

G

-

S

-

K

-

V

-

D

D

P

T

V

P

A

P

V

A

R

E

N

R

T

G

I

V

G

G

M

M

V

V

F

F

Q
|
Q

K

S

A

Y

N

A

P

L

F

Y

P

P

T

H

M

L

S

S

I

V

E

A

D

E

N

N

V

M

V

S

A

F

G

G

L

L

K

K

L

L

S

A

G

G

E

A

K

K

N

K

K

E

-

V

-

I

-

N

K

Q

R

R

L

V

K

N

E

Q

V

V

A

A

E

E

K

-

S

V

L

L

R

Q

G

L

A

A

N

H

L

L

W

-

E

-

V

-

K

-

D

-

R

-

L

L

D

D

K
x
R

P

K

G

P

G

K

G
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

L

V

C

A

I

I

A

G

R

R

A

T

I

L

A

V

V

A

E

E

P

P

E

S

I

V

L

F

L

L

M

L

D

D

E
|
E

P

P

C

L

S

S

A

N

L

L

D

D

P

A

I

A

S

L

T

R

L

V

A

Q

V

M

E

R

D

I

L

E

I

I

H

S

E

R

L

L

K

H

E

K

E

R

F

L

-

G

-

R

T

T

I

M

V

I

I

Y

V

V

T

T

H
|
H

N

D

M

Q

Q

V

Q

E

A

A

A

M

R

T

V

L

S

A

D

D

Q

K

T

I

A

V

F

V

Y

-

S

-

L

L

E

D

A

A

T

-

G

-

R

-

P

-

G

G

R

R

L

V

V

A

E

Q

I

V

G

G

P

K

T

P

K

L

K

E

I

L

F

Y

E

H

S

Y

P

P

D

A

Q

D

K

R

E

F

T

V

E

A

D

G

Y

F

I

I

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y13), S37 (= S38), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (= S43), T43 (= T44), Q81 (= Q91), R128 (≠ K143), A132 (≠ G147), S134 (= S149), G136 (= G151), Q137 (= Q152), E158 (= E173), H191 (= H204)
binding magnesium ion: S42 (= S43), Q81 (= Q91)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
31% identity, 98% coverage: 2:252/257 of query aligns to 1:233/371 of 3puxA

query
sites
3puxA

S

A

K

S

L

V

K

Q

L

L

N

Q

D

N

V

V

N

T

I

K

Y

A

Y
x
W

G

G

D

E

F

V

H

V

A

V

V

S

Q

K

N

D

V

I

N

N

L

L

E

D

V

I

P

H

A

E

R

G

S

E

V

F

T

V

A

V

F

F

I

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

V

L

L

L

R

R

S

M

I

I

N

A

R

G

M

L

H

E

E

T

V

I

T

T

P

S

G

G

A

D

Y

L

V

F

K

I

G

G

E

E

I

K

L

R

L

M

D

N

G

-

E

-

N

-

I

-

Y

-

G

-

S

-

K

-

V

-

D

D

P

T

V

P

A

P

V

A

R

E

N

R

T

G

I

V

G

G

M

M

V

V

F

F

Q
|
Q

K

S

A

Y

N

A

P

L

F

Y

P

P

T

H

M

L

S

S

I

V

E

A

D

E

N

N

V

M

V

S

A

F

G

G

L

L

K

K

L

L

S

A

G

G

E

A

K

K

N

K

K

E

-

V

-

I

-

N

K

Q

R

R

L

V

K

N

E

Q

V

V

A

A

E

E

K

-

S

V

L

L

R

Q

G

L

A

A

N

H

L

L

W

-

E

-

V

-

K

-

D

-

R

-

L

L

D

D

K
x
R

P

K

G

P

G

K

G

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

L

V

C

A

I

I

A

G

R

R

A

T

I

L

A

V

V

A

E

E

P

P

E

S

I

V

L

F

L

L

M

L

D

D

E

E

P

P

C

L

S

S

A

N

L

L

D

D

P

A

I

A

S

L

T

R

L

V

A

Q

V

M

E

R

D

I

L

E

I

I

H

S

E

R

L

L

K

H

E

K

E

R

F

L

-

G

-

R

T

T

I

M

V

I

I

Y

V

V

T

T

H
|
H

N

D

M

Q

Q

V

Q

E

A

A

A

M

R

T

V

L

S

A

D

D

Q

K

T

I

A

V

F

V

Y

-

S

-

L

L

E

D

A

A

T

-

G

-

R

-

P

-

G

G

R

R

L

V

V

A

E

Q

I

V

G

G

P

K

T

P

K

L

K

E

I

L

F

Y

E

H

S

Y

P

P

D

A

Q

D

K

R

E

F

T

V

E

A

D

G

Y

F

I

I

binding adenosine-5'-diphosphate: W12 (≠ Y13), G38 (= G39), C39 (= C40), G40 (= G41), K41 (= K42), S42 (= S43), T43 (= T44), R128 (≠ K143), S134 (= S149), Q137 (= Q152)
binding beryllium trifluoride ion: S37 (= S38), G38 (= G39), K41 (= K42), Q81 (= Q91), S134 (= S149), G136 (= G151), H191 (= H204)
binding magnesium ion: S42 (= S43), Q81 (= Q91)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
31% identity, 98% coverage: 2:252/257 of query aligns to 1:233/371 of 3puwA

query
sites
3puwA

S

A

K

S

L

V

K

Q

L

L

N

Q

D

N

V

V

N

T

I

K

Y

A

Y
x
W

G

G

D

E

F

V

H

V

A
x
V

V

S

Q

K

N

D

V

I

N

N

L

L

E

D

V

I

P

H

A

E

R

G

S

E

V

F

T

V

A

V

F

F

I

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K