SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_053767188.1 NCBI__GCF_001280255.1:WP_053767188.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
56% identity, 92% coverage: 11:291/304 of query aligns to 2:286/304 of Q96XT4

query
sites
Q96XT4

A

V

E

E

K

R

Q

K

P

P

-

V

-

F

-

V

D

D

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

D

Y

F

G

G

I

I

L

L

S

R

A

A

L

E

Q

E

M

M

A

A

L

I

F

R

E

E

L

L

K

G

R

I

D

N

P

P

S

K

Q

S

T

V

A

V

V

I

F

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

A

G

K

K

T

I

P

P

H

H

Y

F

L

M

N

N

-

L

-

P

V

I

Y

S

G

G

V

V

H

H

T

T

L

L

H

H

G

G

R

R

V

S

L

I

P

A

V

F

A

A

Q

T

G

G

A

I

K

K

L

L

A

S

N

N

P

P

H

S

L

L

T

E

V

V

V

I

A

V

V

N

G

V

G

G

D
|
D

G
|
G

D
|
D

G

G

L

L

G

G

I

I

G

G

A

M

G

G

H

H

F

F

V

V

A

H

A

L

G

G

R

R

N

N

V

I

D

D

M

I

L

A

Y

V

I

L

L

V

Y

H

D

N

N
|
N

E

G

V
|
V

Y

Y

G
|
G

L
|
L

T

T

K

K

G

G

Q

Q

A

A

G

S

P

P

T

T

L

L

G

H

L

R

W

G

E

E

K

K

T

T

K

K

S

S

L

L

P

P

K

K

P

P

N

N

P

I

Q

M

S

D

R

A

L

V

N

N

P

P

L

L

L

A

L

V

A

A

F

L

A

A

G

G

Y

Y

T

T

W

F

I

V

G

A

R

R

G

G

Y

Y

A

A

Y

Y

D

D

V

V

K

M

G

H

L

L

K

K

E

E

L

L

I

I

K

K

E

K

G

A

I

I

T

L

H

H

K

K

G

G

L

S

A

A

F

L

L

V

H

D

V

I

L

L

Q

Q

P

P

C
|
C

P

P

T

T

Y

Y

N

N

D

D

L

I

H

N

T

T

K

K

E

E

W

W

F

Y

A

D

P

K

R

R

L

V

Y

Y

K

K

L

L

Q

D

E

N

-

V

E

P

G

G

Y

W

D

D

P

P

H

V

V

V

P

R

E

K

G

-

L

-

P

-

P

E

E

E

L

A

D

Q

R

K

K

K

M

F

S

E

L

Q

F

A

Q

I

E

M

K

K

A

S

L

Y

E

E

W

W

G

G

E

E

R

K

I

I

P

P

V

I

G

G

V

I

F

F

W

Y

K

Q

A

N

E

E

-

L

V

V

P

P

T

T

F

F

G

E

E

D

R

R

L

L

K

T

A

S

Y

N

L

I

P

P

R

N

Y

Y

P

R

E

E

V

Y

Y

Y

P

P

A

A

Q

K

G

Q

Q

Q

T

I

E

E

C12 (= C18) binding [4Fe-4S] cluster
C15 (= C21) binding [4Fe-4S] cluster
IGCS 44:47 (= IGCS 50:53) binding thiamine diphosphate
C46 (= C52) binding [4Fe-4S] cluster
D90 (= D94) binding Mg(2+)
GD 91:92 (= GD 95:96) binding thiamine diphosphate
N118 (= N122) binding Mg(2+)
V120 (= V124) binding Mg(2+)
GL 122:123 (= GL 126:127) binding thiamine diphosphate
C197 (= C201) binding [4Fe-4S] cluster

5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
57% identity, 91% coverage: 16:291/304 of query aligns to 7:283/301 of 5b46B

query
sites
5b46B

D

D

W
|
W

C
|
C

P

P

G

G

C
|
C

G

G

D

D

Y

F

G

G

I

I

L

L

S

R

A

A

L

E

Q

E

M

M

A

A

L

I

F

R

E

E

L

L

K

G

R

I

D

N

P

P

S

K

Q

S

T

V

A

V

V

I

F

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

A

G

K

K

T

I

P

P

H

H

Y

F

L

M

N

N

-

L

-

P

V

I

Y

S

G

G

V

V

H

H

T

T

L

L

H
|
H

G

G

R

R

V

S

L

I

P

A

V

F

A

A

Q

T

G

G

A

I

K

K

L

L

A

S

N

N

P

P

H

S

L

L

T

E

V

V

V

I

A

V

V

N

G

V

G
|
G

D
|
D

G
|
G

D
|
D

G

G

L

L

G

G

I

I

G

G

A

M

G

G

H

H

F

F

V

V

A

H

A

L

G

G

R

R

N

N

V

I

D

D

M

I

L

A

Y

V

I

L

L

V

Y

H

D

N

N
|
N

E

G

V
|
V

Y
|
Y

G
|
G

L
|
L

T
|
T

K

K

G

G

Q

Q

A

A

G

S

P

P

T

T

L

L

G

H

L

R

W

G

E

E

K

K

T

T

K

K

S

S

L

L

P

P

K

K

P

P

N

N

P

I

Q

M

S

D

R

A

L

V

N

N

P

P

L

L

L

A

L

V

A

A

F

L

A

A

G

G

Y

Y

T

T

W

F

I

V

G

A

R

R

G

G

Y

Y

A

A

Y

Y

D

D

V

V

K

M

G

H

L

L

K

K

E

E

L

L

I

I

K

K

E

K

G

A

I

I

T

L

H

H

K

K

G

G

L

S

A

A

F

L

L

V

H

D

V

I

L

L

Q

Q

P

P

C
|
C

P

P

T
|
T

Y
|
Y

N

N

D

D

L

I

H

N

T

T

K

K

E

E

W

W

F

Y

A

D

P

K

R

R

L

V

Y

Y

K

K

L

L

Q

D

E

N

-

V

E

P

G

G

Y

W

D

D

P

P

H

V

V

V

P

R

E

K

G

-

L

-

P

-

P

E

E

E

L

A

D

Q

R

K

K

K

M

F

S

E

L

Q

F

A

Q

I

E

M

K

K

A

S

L

Y

E

E

W

W

G

G

E

E

R

K

I

I

P

P

V

I

G

G

V

I

F

F

W

Y

K

Q

A

N

E

E

-

L

V

V

P

P

T

T

F

F

G

E

E

D

R

R

L

L

K

T

A

S

Y

N

L

I

P

P

R

N

Y

Y

P

R

E

E

V

Y

Y

Y

P

P

A

A

Q

K

G

Q

Q

Q

T

I

E

E

binding magnesium ion: D87 (= D94), N115 (= N122), V117 (= V124)
binding iron/sulfur cluster: W8 (= W17), C9 (= C18), C12 (= C21), C43 (= C52), C194 (= C201), T196 (= T203), Y197 (= Y204)
binding thiamine diphosphate: I41 (= I50), G42 (= G51), C43 (= C52), S44 (= S53), H62 (= H69), G86 (= G93), G88 (= G95), D89 (= D96), N115 (= N122), V117 (= V124), Y118 (= Y125), G119 (= G126), L120 (= L127), T121 (= T128)

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
58% identity, 91% coverage: 16:291/304 of query aligns to 10:286/305 of P72579

query
sites
P72579

D

D

W

W

C

C

P

P

G

G

C

C

G

G

D

N

Y

F

G

G

I

I

L

L

S

N

A

A

L

E

Q

Q

M

Q

A

A

L

I

F

V

E

E

L

L

K

G

R

V

D

D

P

T

S

K

Q

N

T

V

A

V

V

V

F

V

S

S

G

G

I

I

G

G

C

C

S

S

A

G

K
|
K

T

I

P

P

H

H

Y

F

L

F

N

R

-

T

-

P

V

I

Y

S

G

G

V

V

H

H

T

T

L

L

H

H

G

G

R

R

V

A

L

I

P

A

V

F

A

A

Q

T

G

G

A

I

K

K

L

L

A

S

N

N

P

P

H

D

L

L

T

V

V

V

V

I

A

V

V

N

G

G

D

D

G

G

D

D

G

L

L

L

G

G

I

I

G

G

A

A

G

G

H

H

F

F

V

V

A

A

G

G

R

R

N

N

V

V

D

D

M

M

L

V

Y

V

I

I

L

L

Y

H

D

D

N

N

E

G

V

V

Y

Y

G

G

L
|
L

T

T

K

K

G

G

Q

Q

A

A

G

S

P

P

T

T

L

L

G

K

L

R

W

G

E

E

K

K

T

P

K

K

S

S

L

L

P

P

K

R

P

P

N

N

P

I

Q

N

S

D

R

A

L

V

N

N

P

P

L

I

L

A

L

L

A

A

F

I

A

S

G

G

Y

Y

T

T

W

F

I

V

G

A

R

R

G

G

Y

Y

A

A

Y

Y

D

D

V

V

K

K

G

H

L

L

K

K

E

E

L

L

I

I

K

K

E

S

G

A

I

I

T

K

H

H

K

K

G

G

L

L

A

A

F

L

L

I

H

D

V

V

L

L

Q

Q

P

P

C

C

P

P

T

T

Y

Y

N

N

D

D

L

I

H

N

T

T

K

K

E

E

W

W

F

Y

A

D

P

K

R

R

L

I

Y

Y

K

K

L

L

Q

D

E

T

-

L

E

P

G

D

Y

W

D

D

P

P

H

V

V

V

P

K

E

K

G

-

L

-

P

-

P

P

E

E

L

V

D

N

R

E

K

K

M

I

S

K

L

R

F

A

Q

I

E

D

K

K

A

S

L

L

E

E

W

W

G

G

E

D

R

R

I

I

P

P

V

I

G

G

V

I

F

F

W

Y

K

Q

A

N

E

E

-

L

V

V

P

P

T

S

F

Y

G

E

E

E

R

R

L

I

K

K

A

A

Y

N

L

S

P

P

R

A

Y

Y

P

L

E

D

V

Y

Y

T

P

P

A

A

Q

K

G

Q

Q

L

T

I

E

E

K49 (= K55) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (= L127) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
58% identity, 91% coverage: 16:291/304 of query aligns to 10:286/305 of Q96Y68

query
sites
Q96Y68

D

D

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

N

Y

F

G

G

I

I

L

L

S

N

A

A

L

E

Q

Q

M

Q

A

A

L

I

F

V

E

E

L

L

K

G

R

V

D

D

P

T

S

K

Q

N

T

V

A

V

V

V

F

V

S

S

G

G

I

I

G

G

C
|
C

S

S

A

G

K
|
K

T

I

P

P

H

H

Y

F

L

F

N

R

-

T

-

P

V

I

Y

S

G

G

V

V

H

H

T

T

L

L

H

H

G

G

R

R

V

A

L

I

P

A

V

F

A

A

Q

T

G

G

A

I

K

K

L

L

A

S

N

N

P

P

H

D

L

L

T

V

V

V

V

I

A

V

V

N

G

G

D
|
D

G
|
G

D
|
D

G

L

L

L

G

G

I

I

G

G

A

A

G

G

H

H

F

F

V

V

A

A

G

G

R

R

N

N

V

V

D

D

M

M

L

V

Y

V

I

I

L

L

Y

H

D

D

N
|
N

E

G

V
|
V

Y

Y

G
|
G

L
|
L

T

T

K
|
K

G

G

Q

Q

A

A

G

S

P

P

T

T

L

L

G

K

L

R

W

G

E

E

K

K

T

P

K

K

S

S

L

L

P

P

K

R

P

P

N

N

P

I

Q

N

S

D

R

A

L

V

N

N

P

P

L

I

L

A

L

L

A

A

F

I

A

S

G

G

Y

Y

T

T

W

F

I

V

G

A

R

R

G

G

Y

Y

A

A

Y

Y

D

D

V

V

K
|
K

G

H

L

L

K

K

E

E

L

L

I

I

K

K

E

S

G

A

I

I

T

K

H

H

K

K

G

G

L

L

A

A

F

L

L

I

H

D

V

V

L

L

Q

Q

P

P

C
|
C

P

P

T

T

Y

Y

N

N

D

D

L

I

H

N

T

T

K

K

E

E

W

W

F

Y

A

D

P

K

R

R

L

I

Y

Y

K

K

L

L

Q

D

E

T

-

L

E

P

G

D

Y

W

D

D

P

P

H

V

V

V

P

K

E

K

G

-

L

-

P

-

P

P

E

E

L

V

D

N

R

E

K

K

M

I

S

K

L

R

F

A

Q

I

E

D

K

K

A

S

L

L

E

E

W

W

G

G

E

D

R

R

I

I

P

P

V

I

G

G

V

I

F

F

W

Y

K

Q

A

N

E

E

-

L

V

V

P

P

T

S

F

Y

G

E

E

E

R

R

L

I

K

K

A

A

Y

N

L

S

P

P

R

A

Y

Y

P

L

E

D

V

Y

Y

T

P

P

A

A

Q

K

G

Q

Q

L

T

I

E

E

C12 (= C18) binding [4Fe-4S] cluster
C15 (= C21) binding [4Fe-4S] cluster
C46 (= C52) binding [4Fe-4S] cluster
K49 (= K55) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D94) binding Mg(2+)
GD 91:92 (= GD 95:96) binding thiamine diphosphate
N118 (= N122) binding Mg(2+)
V120 (= V124) binding Mg(2+)
GL 122:123 (= GL 126:127) binding thiamine diphosphate
K125 (= K129) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (= K177) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C201) binding [4Fe-4S] cluster

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
56% identity, 91% coverage: 16:291/304 of query aligns to 6:268/286 of 5b48B

query
sites
5b48B

D

D

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

N

Y

F

G

G

I

I

L

L

S

N

A

A

L

E

Q

Q

M

Q

A

A

L

I

F

V

E

E

L

L

K

G

R

V

D

D

P

T

S

K

Q

N

T

V

A

V

V

V

F

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

A

G

K

K

T

I

P

P

H

H

Y

F

L

T

N

P

V

I

Y

S

G

G

V

V

H

H

T

T

L

L

H
|
H

G

G

R

R

V

A

L

I

P

A

V

F

A

A

Q

T

G

G

A

I

K

K

L

L

A

S

N

N

P

P

H

D

L

L

T

V

V

V

V

I

A

V

V

N

G

G

D
|
D

G
|
G

D
|
D

G

L

L

L

G

G

I

I

G

G

A

A

G

G

H

H

F

F

V

V

A

A

G

G

R

R

N

N

V

V

D

D

M

M

L

V

Y

V

I

I

L

L

Y

H

D

D

N
|
N

E

G

V
|
V

Y
|
Y

G
|
G

L
|
L

T

T

K

K

G

G

Q

Q

A

A

G

S

P

P

T

T

L

L

G

K

L

R

W

G

E

E

K

-

T

-

K

-

S

-

L

-

P

-

K

-

P

-

N

N

P

I

Q

N

S

D

R

A

L

V

N

N

P

P

L

I

L

A

L

L

A

A

F

I

A

S

G

G

Y

Y

T

T

W

F

I

V

G

A

R

R

G

G

Y

Y

A

A

Y

Y

D

D

V

V

K

K

G

H

L

L

K

K

E

E

L

L

I

I

K

K

E

S

G

A

I

I

T

K

H

H

K

K

G

G

L

L

A

A

F

L

L

I

H

D

V

V

L

L

Q

Q

P

P

C
|
C

P

P

T
|
T

Y

Y

N

N

D

D

L

I

H

N

T

T

K

K

E

E

W

W

F

-

A

-

P

-

R

R

L

I

Y

Y

K

K

L

L

Q

D

E

T

-

L

E

P

G

D

Y

W

D

D

P

P

H

V

V

V

P

K

E

K

G

-

L

-

P

-

P

P

E

E

L

V

D

N

R

E

K

K

M

I

S

K

L

R

F

A

Q

I

E

D

K

K

A

S

L

L

E

E

W

W

G

G

E

D

R

-

I

I

P

P

V

I

G

G

V

I

F

F

W

Y

K

Q

A

N

E

E

-

L

V

V

P

P

T

S

F

Y

G

E

E

E

R

R

L

I

K

K

A

A

Y

N

L

S

P

P

R

A

Y

Y

P

L

E

D

V

Y

Y

T

P

P

A

A

Q

K

G

Q

Q

L

T

I

E

E

binding magnesium ion: D84 (= D94), N112 (= N122), V114 (= V124)
binding iron/sulfur cluster: C8 (= C18), C11 (= C21), C42 (= C52), C183 (= C201), T185 (= T203)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I50), G41 (= G51), C42 (= C52), S43 (= S53), H59 (= H69), G85 (= G95), D86 (= D96), N112 (= N122), V114 (= V124), Y115 (= Y125), G116 (= G126), L117 (= L127)

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
51% identity, 91% coverage: 16:291/304 of query aligns to 3:257/275 of 5b47B

query
sites
5b47B

D

D

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

D

Y

F

G

G

I

I

L

L

S

R

A

A

L

E

Q

E

M

M

A

A

L

I

F

R

E

E

L

L

K

G

R

I

D

N

P

P

S

K

Q

S

T

V

A

V

V

I

F

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

A

G

K

K

T

I

P

P

H

H

Y

F

L

M

N

N

-

L

-

P

V

I

Y

S

G

G

V

V

H

H

T

T

L

L

H
|
H

G

G

R

R

V

S

L

I

P

A

V

F

A

A

Q

T

G

G

A

I

K

K

L

L

A

S

N

N

P

P

H

S

L

L

T

E

V

V

V

I

A

V

V

N

G

V

G

G

D
|
D

G
|
G

D
|
D

G

G

L

L

G

G

I

I

G

G

A

M

G

G

H

H

F

F

V

V

A

H

A

L

G

G

R

R

N

N

V

I

D

D

M

I

L

A

Y

V

I

L

L

V

Y

H

D

N

N
|
N

E

G

V
|
V

Y
|
Y

G

G

L
|
L

T

-

K

-

G

-

Q

Q

A

A

G

S

P

P

T

T

L

L

G

H

L

R

W

G

E

E

K

-

T

-

K

-

S

-

L

-

P

-

K

-

P

-

N

N

P

I

Q

M

S

D

R

A

L

V

N

N

P

P

L

L

L

A

L

V

A

A

F

L

A

A

G

G

Y

Y

T

T

W

F

I

V

G

A

R

R

G

G

Y

Y

A

A

Y

Y

D

D

V

V

K

M

G

H

L

L

K

K

E

E

L

L

I

I

K

K

E

K

G

A

I

I

T

L

H

H

K

K

G

G

L

S

A

A

F

L

L

V

H

D

V

I

L

L

Q

Q

P

P

C
|
C

P

P

T

T

Y

Y

N

-

D

-

L

-

H

-

T

-

K

-

E

-

W

-

F

-

A

-

P

-

R

R

L

V

Y

Y

K

K

L

L

Q

D

E

N

-

V

E

P

G

G

Y

W

D

D

P

P

H

V

V

V

P

R

E

K

G

-

L

-

P

-

P

E

E

E

L

A

D

Q

R

K

K

K

M

F

S

E

L

Q

F

A

Q

I

E

M

K

K

A

S

L

Y

E

E

W

W

G

G

E

E

R

K

I

I

P

P

V

I

G

G

V

I

F

F

W

Y

K

Q

A

N

E

E

-

L

V

V

P

P

T

T

F

F

G

E

E

D

R

R

L

L

K

T

A

S

Y

N

L

I

P

P

R

N

Y

Y

P

R

E

E

V

Y

Y

Y

P

P

A

A

Q

K

G

Q

Q

Q

T

I

E

E

binding magnesium ion: D83 (= D94), N111 (= N122)
binding iron/sulfur cluster: C5 (= C18), C8 (= C21), C39 (= C52), C179 (= C201)
binding thiamine diphosphate: I37 (= I50), G38 (= G51), C39 (= C52), S40 (= S53), H58 (= H69), G84 (= G95), D85 (= D96), N111 (= N122), V113 (= V124), Y114 (= Y125), L116 (= L127)

6n2oD 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
37% identity, 90% coverage: 1:273/304 of query aligns to 8:267/291 of 6n2oD

query
sites
6n2oD

M

M

V

E

E

N

L

M

K

K

L

P

A

K

D

D

Y

Y

K

K

A

S

E

E

K

V

Q

P

P

T

D

T

W
|
W

C
|
C

P

P

G

G

C
|
C

G

G

D

H

Y

F

G

G

I

I

L

L

S

N

A

G

L

V

Q

Y

M

R

A

A

L

M

F

A

E

E

L

L

K

G

R

I

D

D

P

S

S

T

Q

K

T

F

A

A

V

A

F

I

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

A

S

K
x
R

T

M

P

P

H

Y

Y

F

L

V

N

D

V

S

Y

Y

G

K

V

M

H

H

T

T

L

L

H
|
H

G

G

R

R

V

A

L

G

P

A

V

V

A

A

Q

T

G

G

A

T

K

Q

L

V

A

A

N

R

P

P

H

D

L

L

T

C

V

V

A

V

V

A

G

G

D
|
D

G
|
G

D
|
D

G

G

L

F

G

S

I

I

G

G

A

G

G

G

H

H

F

M

V

P

A

H

A

M

G

A

R

R

R

K

N

N

V

V

D

N

M

M

L

T

Y

Y

I

V

L

L

Y

M

D

D

N
|
N

E

G

V
x
I

Y

Y

G
|
G

L
|
L

T
|
T

K
|
K

G

G

Q

Q

A

Y

G

S

P

P

T

T

L

S

G

R

L

P

W

E

E

M

K

T

T

A

K

Y

S

T

L

T

P

P

K

Y

P

G

N

G

P

P

Q

E

S

N

R

P

L

M

N

N

P

P

L

L

L

Y

A

M

F

L

A

T

A

Y

G

G

Y

A

T

T

W

Y

I

V

G

A

R

Q

G

A

Y

F

A

A

Y

G

D

K

V

P

K

K

G

D

L

C

K

A

E

E

L

L

I

I

K

K

E

G

G

A

I

M

T

E

H

H

K

E

G

G

L

F

A

A

F

Y

L

V

H

N

V

I

L

F

Q

S

P

Q

C
|
C

P

P

T
|
T

Y
x
F

N

N

D

K

L

I

H

D

T

T

K

V

E

D

W

F

F

Y

A

R

P

D

R

L

L

V

Y

-

K

-

L

-

Q

-

E

-

G

-

Y

-

D

-

P

-

H

-

V

-

P

-

E

E

G

P

L

I

P

P

P

E

E

D

E

H

L

D

D

T

R

S

K

D

M

L

S

G

L

A

F

A

Q

M

E

E

K

L

A

A

L

R

E

R

W

P

G

G

E

G

R

K

I

A

P

P

V

T

G

G

V

L

F

L

W

Y

K

K

A

T

E

S

V

A

P

P

T

T

F

L

G

D

E

Q

R

N

L

L

binding magnesium ion: D101 (= D94), N129 (= N122), I131 (≠ V124)
binding succinyl-coenzyme a: I57 (= I50), R62 (≠ K55), L134 (= L127), K136 (= K129)
binding iron/sulfur cluster: W24 (= W17), C25 (= C18), C28 (= C21), C59 (= C52), C208 (= C201), T210 (= T203), F211 (≠ Y204)
binding thiamine diphosphate: I57 (= I50), G58 (= G51), C59 (= C52), S60 (= S53), H76 (= H69), G102 (= G95), D103 (= D96), N129 (= N122), I131 (≠ V124), G133 (= G126), L134 (= L127), T135 (= T128)

6n2oB 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
37% identity, 90% coverage: 1:273/304 of query aligns to 8:267/291 of 6n2oB

query
sites
6n2oB

M

M

V

E

E

N

L

M

K

K

L

P

A

K

D

D

Y

Y

K

K

A

S

E

E

K

V

Q

P

P

T

D

T

W
|
W

C
|
C

P

P

G

G

C
|
C

G

G

D

H

Y

F

G

G

I

I

L

L

S

N

A

G

L

V

Q

Y

M

R

A

A

L

M

F

A

E

E

L

L

K

G

R

I

D

D

P

S

S

T

Q

K

T

F

A

A

V

A

F

I

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

A

S

K
x
R

T

M

P

P

H

Y

Y

F

L

V

N

D

V

S

Y

Y

G

K

V

M

H

H

T

T

L

L

H
|
H

G

G

R

R

V

A

L

G

P

A

V

V

A

A

Q

T

G

G

A

T

K

Q

L

V

A

A

N

R

P

P

H

D

L

L

T

C

V

V

A

V

V

A

G

G

D
|
D

G
|
G

D
|
D

G

G

L

F

G

S

I

I

G

G

A

G

G

G

H

H

F

M

V

P

A

H

A

M

G

A

R

R

R

K

N

N

V

V

D

N

M

M

L

T

Y

Y

I

V

L

L

Y

M

D

D

N
|
N

E

G

V
x
I

Y
|
Y

G
|
G

L
|
L

T
|
T

K
|
K

G

G

Q

Q

A

Y

G

S

P

P

T

T

L

S

G

R

L

P

W

E

E

M

K

T

T

A

K
x
Y

S

T

L

T

P

P

K

Y

P

G

N

G

P

P

Q

E

S

N

R

P

L

M

N

N

P

P

L

L

L

Y

A

M

F

L

A

T

A

Y

G

G

Y

A

T

T

W

Y

I

V

G

A

R

Q

G

A

Y

F

A

A

Y

G

D

K

V

P

K

K

G

D

L

C

K

A

E

E

L

L

I

I

K

K

E

G

G

A

I

M

T

E

H

H

K

E

G

G

L

F

A

A

F

Y

L

V

H

N

V

I

L

F

Q

S

P

Q

C
|
C

P

P

T
|
T

Y
x
F

N

N

D

K

L

I

H

D

T

T

K

V

E

D

W

F

F

Y

A

R

P

D

R

L

L

V

Y

-

K

-

L

-

Q

-

E

-

G

-

Y

-

D

-

P

-

H

-

V

-

P

-

E

E

G

P

L

I

P

P

P

E

E

D

E

H

L

D

D

T

R

S

K

D

M

L

S

G

L

A

F

A

Q

M

E

E

K

L

A

A

L

R

E

R

W

P

G

G

E

G

R

K

I

A

P

P

V

T

G

G

V

L

F

L

W

Y

K

K

A

T

E

S

V

A

P

P

T

T

F

L

G

D

E

Q

R

N

L

L

binding 2-oxoglutaric acid: R62 (≠ K55), L134 (= L127)
binding coenzyme a: K136 (= K129), Y150 (≠ K143)
binding magnesium ion: D101 (= D94), N129 (= N122), I131 (≠ V124)
binding iron/sulfur cluster: W24 (= W17), C25 (= C18), C28 (= C21), C59 (= C52), C208 (= C201), T210 (= T203), F211 (≠ Y204)
binding thiamine diphosphate: I57 (= I50), G58 (= G51), C59 (= C52), S60 (= S53), H76 (= H69), G102 (= G95), D103 (= D96), N129 (= N122), I131 (≠ V124), Y132 (= Y125), G133 (= G126), L134 (= L127), T135 (= T128)

6n2nB Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
37% identity, 90% coverage: 1:273/304 of query aligns to 8:267/291 of 6n2nB

query
sites
6n2nB

M

M

V

E

E

N

L

M

K

K

L

P

A

K

D

D

Y

Y

K

K

A

S

E

E

K

V

Q

P

P

T

D

T

W
|
W

C
|
C

P

P

G

G

C
|
C

G

G

D
x
H

Y

F

G

G

I

I

L

L

S

N

A

G

L

V

Q

Y

M

R

A

A

L

M

F

A

E

E

L

L

K

G

R

I

D

D

P

S

S

T

Q

K

T

F

A

A

V

A

F

I

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

A

S

K

R

T

M

P

P

H

Y

Y

F

L

V

N

D

V

S

Y

Y

G

K

V

M

H

H

T

T

L

L

H
|
H

G

G

R

R

V

A

L

G

P

A

V

V

A

A

Q

T

G

G

A

T

K

Q

L

V

A

A

N

R

P

P

H

D

L

L

T

C

V

V

A

V

V

A

G

G

G
|
G

D
|
D

G
|
G

D
|
D

G

G

L

F

G

S

I

I

G

G

A

G

G

G

H

H

F

M

V

P

A

H

A

M

G

A

R

R

R

K

N

N

V

V

D

N

M

M

L

T

Y

Y

I

V

L

L

Y

M

D

D

N
|
N

E

G

V
x
I

Y
|
Y

G
|
G

L
|
L

T
|
T

K

K

G

G

Q

Q

A

Y

G

S

P

P

T

T

L

S

G

R

L

P

W

E

E

M

K

T

T

A

K

Y

S

T

L

T

P

P

K

Y

P

G

N

G

P

P

Q

E

S

N

R

P

L

M

N

N

P

P

L

L

L

Y

A

M

F

L

A

T

A

Y

G

G

Y

A

T

T

W

Y

I

V

G

A

R

Q

G

A

Y

F

A

A

Y

G

D

K

V

P

K

K

G

D

L

C

K

A

E

E

L

L

I

I

K

K

E

G

G

A

I

M

T

E

H

H

K

E

G

G

L

F

A

A

F

Y

L

V

H

N

V

I

L

F

Q

S

P

Q

C
|
C

P

P

T
|
T

Y
x
F

N

N

D

K

L

I

H

D

T

T

K

V

E

D

W

F

F

Y

A

R

P

D

R

L

L

V

Y

-

K

-

L

-

Q

-

E

-

G

-

Y

-

D

-

P

-

H

-

V

-

P

-

E

E

G

P

L

I

P

P

P

E

E

D

E

H

L

D

D

T

R

S

K

D

M

L

S

G

L

A

F

A

Q

M

E

E

K

L

A

A

L

R

E

R

W

P

G

G

E

G

R

K

I

A

P

P

V

T

G

G

V

L

F

L

W

Y

K

K

A

T

E

S

V

A

P

P

T

T

F

L

G

D

E

Q

R

N

L

L

binding magnesium ion: D101 (= D94), N129 (= N122), I131 (≠ V124)
binding iron/sulfur cluster: W24 (= W17), C25 (= C18), C28 (= C21), H30 (≠ D23), C59 (= C52), C208 (= C201), T210 (= T203), F211 (≠ Y204)
binding thiamine diphosphate: I57 (= I50), G58 (= G51), C59 (= C52), S60 (= S53), H76 (= H69), G100 (= G93), D101 (= D94), G102 (= G95), D103 (= D96), N129 (= N122), I131 (≠ V124), Y132 (= Y125), G133 (= G126), L134 (= L127), T135 (= T128)

5exeC Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
29% identity, 38% coverage: 87:202/304 of query aligns to 103:226/314 of 5exeC

query
sites
5exeC

T

N

V

I

A

V

V

M

G

A

G
|
G

D
|
D

G
|
G

D

G

G

A

L

V

G

D

I

I

G

G

A

L

G

Q

H

A

F

L

V

S

A

A

A

M

G

L

R

Y

R

R

N

G

V

H

D
|
D

M

V

L

L

Y

F

I

I

L

C

Y
|
Y

D

D

N
|
N

E

E

V
x
S

Y
|
Y

G
x
A

L
x
N

T
|
T

K

G

G

I

Q

Q

A

T

G

S

P

P

T

T

L

T

G

P

L

Y

W

G

E

A

K

N

T

T

-

T

-

F

-

T

-

P

-

G

-

E

-

V

-

P

-

E

-

G

-

K

K

K

S

L

L

F

P

P

K

K

P

D

N

N

P

P

Q

K

-

V

-

I

S

A

R

H

L

G

N

H

P

P

L

E

L

L

L

K

A

Y

F

V

A

A

A

T

G

A

Y

S

T

I

W

-

I

-

G

-

R

-

G

G

Y

W

A

P

Y

V

D

D

V

-

K

-

G

-

L

L

K

M

E

N

L

K

I

V

K

R

E

K

G

G

I
x
L

T

N

H
x
Q

K

E

G

G

L

P

A

A

F

Y

L

I

H

H

V

I

L

H

Q

A

P

P

C
|
C

P
|
P

active site: N143 (≠ L127)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: G109 (= G93), D110 (= D94), G111 (= G95), Y136 (= Y120), N138 (= N122), S140 (≠ V124), Y141 (= Y125), A142 (≠ G126), N143 (≠ L127), T144 (= T128)
binding magnesium ion: D110 (= D94), D130 (= D114), N138 (= N122), S140 (≠ V124), L211 (≠ I187), Q213 (≠ H189)
binding iron/sulfur cluster: C225 (= C201), P226 (= P202)

Sites not aligning to the query:

5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
29% identity, 38% coverage: 87:202/304 of query aligns to 103:226/310 of 5exdF

query
sites
5exdF

T

N

V

I

A

V

V

M

G

A

G

G

D
|
D

G
|
G

D

G

G

A

L

V

G

D

I

I

G

G

A

L

G

Q

H

A

F

L

V

S

A

A

A

M

G

L

R

Y

R

R

N

G

V

H

D

D

M

V

L

L

Y

F

I

I

L

C

Y
|
Y

D

D

N
|
N

E

E

V
x
S

Y
|
Y

G
x
A

L
x
N

T
|
T

K

G

G

I

Q

Q

A

T

G

S

P

P

T

T

L

T

G

P

L

Y

W

G

E

A

K

N

T

T

-

T

-

F

-

T

-

P

-

G

-

E

-

V

-

P

-

E

-

G

-

K

K

K

S

L

L

F

P

P

K

K

P

D

N

N

P

P

Q

K

-

V

-

I

S

A

R

H

L

G

N

H

P

P

L

E

L

L

L

K

A

Y

F

V

A

A

A

T

G

A

Y

S

T

I

W

-

I

-

G

-

R

-

G

G

Y

W

A

P

Y

V

D

D

V

-

K

-

G

-

L

L

K

M

E

N

L

K

I

V

K

R

E

K

G

G

I

L

T

N

H

Q

K

E

G

G

L

P

A

A

F

Y

L

I

H

H

V

I

L

H

Q

A

P

P

C
|
C

P

P

active site: N143 (≠ L127)
binding magnesium ion: D110 (= D94), N138 (= N122), S140 (≠ V124)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: D110 (= D94), G111 (= G95), Y136 (= Y120), N138 (= N122), S140 (≠ V124), Y141 (= Y125), A142 (≠ G126), N143 (≠ L127), T144 (= T128)
binding iron/sulfur cluster: A142 (≠ G126), C225 (= C201)

Sites not aligning to the query:

5c4iC Structure of an oxalate oxidoreductase (see paper)
29% identity, 38% coverage: 87:202/304 of query aligns to 103:226/312 of 5c4iC

query
sites
5c4iC

T

N

V

I

A

V

V

M

G

A

G
|
G

D
|
D

G
|
G

D

G

G

A

L

V

G

D

I

I

G

G

A

L

G

Q

H

A

F

L

V

S

A

A

A

M

G

L

R

Y

R

R

N

G

V

H

D

D

M

V

L

L

Y

F

I

I

L

C

Y
|
Y

D

D

N
|
N

E

E

V
x
S

Y
|
Y

G
x
A

L
x
N

T
|
T

K

G

G

I

Q

Q

A

T

G

S

P

P

T

T

L

T

G

P

L

Y

W

G

E

A

K

N

T

T

-

T

-

F

-

T

-

P

-

G

-

E

-

V

-

P

-

E

-

G

-

K

K

K

S

L

L

F

P

P

K

K

P

D

N

N

P

P

Q

K

-

V

-

I

S

A

R

H

L

G

N

H

P

P

L

E

L

L

L

K

A

Y

F

V

A

A

A

T

G

A

Y

S

T

I

W

-

I

-

G

-

R

-

G

G

Y

W

A

P

Y

V

D

D

V

-

K

-

G

-

L

L

K

M

E

N

L

K

I

V

K

R

E

K

G

G

I

L

T

N

H

Q

K

E

G

G

L

P

A

A

F

Y

L

I

H

H

V

I

L

H

Q

A

P

P

C
|
C

P
|
P

active site: N143 (≠ L127)
binding magnesium ion: D110 (= D94), N138 (= N122), S140 (≠ V124)
binding iron/sulfur cluster: A142 (≠ G126), C225 (= C201), P226 (= P202)
binding thiamine diphosphate: G109 (= G93), D110 (= D94), G111 (= G95), Y136 (= Y120), N138 (= N122), S140 (≠ V124), Y141 (= Y125), A142 (≠ G126), N143 (≠ L127), T144 (= T128)

Sites not aligning to the query:

binding iron/sulfur cluster: 24, 27, 29, 52, 227
binding thiamine diphosphate: 50, 51, 52, 74

Query Sequence

>WP_053767188.1 NCBI__GCF_001280255.1:WP_053767188.1
MVELKLADYKAEKQPDWCPGCGDYGILSALQMALFELKRDPSQTAVFSGIGCSAKTPHYL
NVYGVHTLHGRVLPVAQGAKLANPHLTVVAVGGDGDGLGIGAGHFVAAGRRNVDMLYILY
DNEVYGLTKGQAGPTLGLWEKTKSLPKPNPQSRLNPLLLAFAAGYTWIGRGYAYDVKGLK
ELIKEGITHKGLAFLHVLQPCPTYNDLHTKEWFAPRLYKLQEEGYDPHVPEGLPPEELDR
KMSLFQEKALEWGERIPVGVFWKAEVPTFGERLKAYLPRYPEVYPAQGQTEALDLEGLLK
EFAL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory