SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_053767937.1 NCBI__GCF_001280255.1:WP_053767937.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
51% identity, 98% coverage: 2:552/561 of query aligns to 95:660/667 of P09342

query
sites
P09342

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C161 (≠ V67) modified: Disulfide link with 307
P194 (= P100) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ A211) modified: Disulfide link with 161

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
51% identity, 98% coverage: 2:552/561 of query aligns to 92:657/664 of P09114

query
sites
P09114

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P191 (= P100) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W469) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
50% identity, 98% coverage: 2:552/561 of query aligns to 12:577/582 of 3ea4A

query
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3ea4A

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Q

A

Q

E

L

F

A

T

I

G

P

E

N

F

W

D

E

V

Q

E

A

L

M

D

R

L

L

P

P

G

G

Y

Y

-

M

-

S

-

R

-

M

-

P

K

K

P

P

T

P

L

E

R

D

G

S

H

H

P

L

K

E

Q

Q

I

I

E

V

R

R

A

-

L

-

D

-

A

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L

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S

K

E

A

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K

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P

I

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G

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C

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A

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T

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M

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L

G

G

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P

G

C

N

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x
L

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G

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H
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H

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G

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V

A

Y

A

A

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N

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H

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G

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R

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D

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K

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E

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A

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-

I

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H

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D

I
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I

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A

A

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E

I

I

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G

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I

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G

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x
V

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K

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L

V

A

L

L

K

Q

E

G

M

M

L

N

K

K

-

V

-

L

-

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-

N

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A

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P

E

L

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K

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-

D

-

F

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W

W

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E

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W

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Q

R

K

Y

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P

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Y

F

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K

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T

R

F

P

G

H

E

L

A

Q

I

A

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P

P

E

Q

-

Y

V

A

I

I

R

K

A

V

F

L

H

D

E

E

A

L

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T

G

D

G

G

N

K

A

A

I

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I

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G

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Q

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H

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F

W

A

A

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Y

F

F

Y

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V

Y

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K

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G

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G

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G

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L

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P

F

A

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G

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I

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D

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G

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D

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S

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F

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I

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M

T

N

L

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Q

E

E

L

L

A

A

T

T

V

I

V

R

K

V

Y

E

R

N

L

L

P

P

V

V

K

K

V

V

V

L

I

L

L

L

N

N

N
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x
H

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L

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G

M
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M

V
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R

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W

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D

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F

H

Y

A

K

K

A

R

N

Y

R

S

A

E

H

V

T

Y

F

L

L

A

G

D

D

S

P

N

A

-

Q

-

E

-

D

-

E

-

I

-

F

P

P

D

N

F

M

A

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

I

I

K

P

G

A

V

A

R

R

V

V

E

T

R

K

K

K

E

A

D

D

L

L

K

R

K

E

G

A

V

I

E

Q

A

T

V

M

L

L

N

D

A

T

D

P

G

G

P

P

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Y

V

L

A

L

E

D

F

V

K

I

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C

Y

P

H
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H

E

Q

E

E

G

H

V

V

F

L

P

P

M

M

I

I

P

P

A
x
S

G

G

A

T

A

F

E

N

D

D

M

V

I

I

L

T

E

E

active site: Y32 (≠ H22), G34 (= G24), G35 (= G25), A36 (= A26), S37 (≠ I27), E58 (= E47), T81 (= T70), F120 (= F109), Q121 (= Q110), E122 (= E111), K170 (= K159), M265 (= M252), V292 (= V279), V399 (= V380), G425 (= G406), M427 (= M408), D452 (= D433), N479 (= N460), H481 (≠ Y462), L482 (= L463), M484 (= M465), V485 (= V466), W488 (= W469), H557 (= H532)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (= D277), R291 (= R278), W488 (= W469), S567 (≠ A542)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R149), G221 (= G208), G222 (= G209), G223 (= G210), T245 (= T232), L246 (= L233), M247 (= M234), L263 (≠ P250), G264 (= G251), M265 (= M252), H266 (= H253), G285 (= G272), R287 (= R274), D289 (= D276), R291 (= R278), D309 (= D297), I310 (= I298), G327 (= G315), D328 (= D316), V329 (≠ S317), M404 (= M385), G422 (= G403)
binding magnesium ion: D452 (= D433), N479 (= N460), H481 (≠ Y462)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V380), G400 (= G381), Q401 (= Q382), H402 (= H383), M427 (= M408), G451 (= G432), D452 (= D433), G453 (= G434), S454 (= S435), N479 (= N460), H481 (≠ Y462), L482 (= L463), G483 (= G464), M484 (= M465), V485 (= V466)

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
50% identity, 98% coverage: 2:552/561 of query aligns to 12:577/582 of 3e9yA

query
sites
3e9yA

K

K

G

G

A

A

E

D

A

I

L

L

L

V

K

E

A

A

L

L

E

E

R

R

E

Q

G

G

V

V

E

E

V

T

I

V

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F

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A

H
x
Y

P

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G
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G

A
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A

I
x
S

M

M

P

E

T

I

Y

H

D

Q

A

A

L

L

Y

T

-

R

D

S

S

S

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S

I

I

R

R

H

N

I

V

L

L

V

P

R

R

H

H

E
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E

Q

Q

G

G

I

V

H

F

A

A

T

E

A

G

Y

Y

A

A

R

R

A

S

S

S

G

G

R

K

V

P

G

G

V

I

V

C

M

I

A

A

T
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T

S

S

G

G

P

P

G

G

A

A

L

T

N

N

L

L

V

V

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S

G

G

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L

A

A

D

D

A

A

L

L

M

L

D

D

S

S

T

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P

V

L

V

V

A

A

I

I

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T

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N

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V

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R

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I

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G

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T

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D

A

A

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E
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E

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P

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E

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V

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I

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K

K

H

H

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N

Y

Y

L

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D

D

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N

E

E

D

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I

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P

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E

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E

A

A

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F

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A

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R

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K

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D

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P

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G

Y

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K

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-

A

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G

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L

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M

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A

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I
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I

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x
V

R

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L

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M

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N

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K

-

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N

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L

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L

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-

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Y

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K

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F

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Y

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Y

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A

A

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D

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S
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S

F

F

Q

I

M

M

T

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L

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Q

Q

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E

L

L

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A

T

T

V

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V

Y

E

R

N

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L

P

P

V

V

K

K

V

V

V

L

I

L

L

L

N

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N
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N

G

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x
H

L
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L

G
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M
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M

V
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V

R

M

Q

Q

W
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W

Q

E

D

D

L

R

F

F

H

Y

A

K

K

A

R

N

Y

R

S

A

E

H

V

T

Y

F

L

L

A

G

D

D

S

P

N

A

-

Q

-

E

-

D

-

E

-

I

-

F

P

P

D

N

F

M

A

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

I

I

K

P

G

A

V

A

R

R

V

V

E

T

R

K

K

K

E

A

D

D

L

L

K

R

K

E

G

A

V

I

E

Q

A

T

V

M

L

L

N

D

A

T

D

P

G

G

P

P

V

Y

V

L

A

L

E

D

F

V

K

I

V

C

Y

P

H
|
H

E

Q

E

E

G

H

V

V

F

L

P

P

M

M

I

I

P

P

A
x
S

G

G

A

T

A

F

E

N

D

D

M

V

I

I

L

T

E

E

active site: Y32 (≠ H22), G34 (= G24), G35 (= G25), A36 (= A26), S37 (≠ I27), E58 (= E47), T81 (= T70), F120 (= F109), Q121 (= Q110), E122 (= E111), K170 (= K159), M265 (= M252), V292 (= V279), V399 (= V380), G425 (= G406), M427 (= M408), D452 (= D433), N479 (= N460), H481 (≠ Y462), L482 (= L463), M484 (= M465), V485 (= V466), W488 (= W469), H557 (= H532)
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (= D277), R291 (= R278), W488 (= W469), S567 (≠ A542)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R149), G221 (= G208), G222 (= G209), G223 (= G210), T245 (= T232), L246 (= L233), M247 (= M234), L263 (≠ P250), G285 (= G272), R287 (= R274), D289 (= D276), R291 (= R278), D309 (= D297), I310 (= I298), G327 (= G315), D328 (= D316), V329 (≠ S317), M404 (= M385), G422 (= G403)
binding magnesium ion: D452 (= D433), N479 (= N460), H481 (≠ Y462)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V380), G400 (= G381), Q401 (= Q382), H402 (= H383), M427 (= M408), G451 (= G432), G453 (= G434), S454 (= S435), N479 (= N460), H481 (≠ Y462), L482 (= L463), G483 (= G464), M484 (= M465), V485 (= V466)

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
49% identity, 98% coverage: 2:552/561 of query aligns to 98:663/670 of P17597

query
sites
P17597

K

K

G

G

A

A

E

D

A

I

L

L

L

V

K

E

A

A

L

L

E

E

R

R

E

Q

G

G

V

V

E

E

V

T

I

V

F

F

G

A

H

Y

P

P

G

G

A
|
A

I

S

M
|
M

P

E

T

I

Y

H

D

Q

A

A

L

L

Y

T

-

R

D

S

S

S

P

S

I

I

R

R

H

N

I

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

G

G

I

V

H

F

A

A

T

E

A

G

Y

Y

A

A

R

R

A

S

S

S

G

G

R

K

V

P

G

G

V

I

V

C

M

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

L

T

N

N

L

L

V

V

T

S

G

G

L

L

A

A

D

D

A

A

L

L

M

L

D

D

S
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S

T

V

P

P

V

L

V

V

A

A

I

I

T

T

G

G

N

Q

V

V

P
|
P

R

R

A
x
R

L

M

I

I

G

G

T

T

D

D

A

A

F

F

Q
|
Q

E

E

A

T

D

P

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T

V

G

E

V

V

T

T

M

R

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S

I

I

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T

K
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K

H

H

N

N

Y

Y

L

L

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V

Q

M

D

D

V

V

N

E

E

D

I

I

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P

R

R

V

I

K

E

E

E

A

A

F

F

H

F

I

L

A

A

S

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

I

V

D

D

L

V

P

P

K
|
K

D

D

V

I

Q

Q

L

Q

A

Q

E

L

F

A

T

I

G

P

E

N

F

W

D

E

V

Q

E

A

L

M

D

R

L

L

P

P

G

G

Y

Y

-

M

-

S

-

R

-

M

-

P

K

K

P

P

T

P

L

E

R

D

G

S

H

H

P

L

K

E

Q

Q

I

I

E

V

R

R

A

-

L

-

D

-

A

L

L

I

E

S

K

E

A

S

E

K

R

K

P

P

I

V

L

L

M

Y

V

V

G

G

G
|
G

G

G

A

C

Q

L

H

N

A

S

H

S

A

D

E

E

L

L

L

G

A

R

F

F

A

V

E

E

K

L

T

T

G

G

L

I

P

P

V

V

I

A

T

S

T
|
T

L
|
L

M

M

G

G

L

L

G

G

A

S

F

Y

P

P

G
x
C

N

D

H

D

P

E

L

L

W

S

L

L

G

H

M

M

P
x
L

G
|
G

M
|
M

H
|
H

G

G

T

T

V

V

A

Y

A

A

N

N

R

Y

A

A

I

V

H

E

H

H

A

S

D

D

V

L

I

L

L

L

A

A

I

F

G
|
G

L
x
V

R
|
R

F
|
F

D
|
D

R
|
R

V

V

T

T

G

G

K

K

V

L

S

E

R

A

F

F

A

A

P

S

H

R

A

A

H

K

T

-

I

I

I

V

H

H

V

I

D
|
D

I
|
I

D

D

P

S

A

A

E

E

I

I

G

G

K

K

V

N

V

K

R

T

T

P

H

H

I

V

P

S

I

V

V

C

G

G

D
|
D

S
x
V

R

K

L

L

V

A

L

L

K

Q

E

G

M

M

L

N

K

K

-

V

-

L

-

E

-

N

G

R

A

A

K

E

P

E

L

L

R

K

L

L

-

D

-

F

A

G

A

V

W

W

W

R

R

N

E

E

L

L

E

N

E

V

W

Q

R

K

T

Q

R

K

Y

F

P

P

L

L

R

S

Y

F

R

K

P

T

R

F

P

G

H

E

L

A

Q

I

A

P

P

P

E

Q

-

Y

V

A

I

I

R

K

A

V

F

L

H

D

E

E

A

L

T

T

G

D

G

G

N

K

A

A

I

I

V

I

T

S

T

T

G

G

V

V

G

G

Q
|
Q

H
|
H

Q

Q

M

M

F

W

A

A

Q

Q

Y

F

F

Y

T

N

V

Y

T

K

R

K

P

P

R

R

S

Q

F

W

L

L

T

S

S

S

G
|
G

L

L

G
|
G

T
x
A

M
|
M

G

G

V

F

G

G

L

L

P

P

F

A

A

A

I

I

G

G

A

A

Q

S

I

V

A

A

R

N

P

P

E

D

E

A

L

I

V

V

I

V

D

D

F

I

D

D

G

G

D
|
D

G
|
G

S
|
S

F

F

Q

I

M

M

T

N

L

V

Q

Q

E

E

L

L

A

A

T

T

V

I

V

R

K

V

Y

E

R

N

L

L

P

P

V

V

K

K

V

V

V

L

I

L

L

L

N

N

N
|
N

G
x
Q

Y
x
H

L
|
L

G
|
G

M
|
M

V

V

R

M

Q

Q

W
|
W

Q

E

D

D

L

R

F

F

H

Y

A

K

K

A

R

N

Y

R

S

A

E

H

V

T

Y

F

L

L

A

G

D

D

S

P

N

A

-

Q

-

E

-

D

-

E

-

I

-

F

P

P

D

N

F

M

A

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

I

I

K

P

G

A

V

A

R

R

V

V

E

T

R

K

K

K

E

A

D

D

L

L

K

R

K

E

G

A

V

I

E

Q

A

T

V

M

L

L

N

D

A

T

D

P

G

G

P

P

V

Y

V

L

A

L

E

D

F

V

K

I

V

C

Y

P

H

H

E

Q

E

E

G

H

V

V

F

L

P

P

M

M

I

I

P

P

A
x
S

G

G

A

T

A

F

E

N

D

D

M

V

I

I

L

T

E

E

A122 (= A26) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (= M28) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E47) binding thiamine diphosphate
S186 (= S89) binding FAD
P197 (= P100) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ A102) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q110) binding thiamine diphosphate
K220 (= K123) binding (R)-imazaquin
R246 (= R149) binding (R)-imazaquin; binding FAD
K256 (= K159) binding chlorimuron-ethyl
G308 (= G209) binding FAD
TL 331:332 (= TL 232:233) binding FAD
C340 (≠ G241) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (≠ PGMH 250:253) binding FAD
GVRFD 371:375 (≠ GLRFD 272:276) binding FAD
DR 376:377 (= DR 277:278) binding chlorimuron-ethyl
DI 395:396 (= DI 297:298) binding FAD
DV 414:415 (≠ DS 316:317) binding FAD
QH 487:488 (= QH 382:383) binding thiamine diphosphate
GG 508:509 (= GG 403:404) binding FAD
GAM 511:513 (≠ GTM 406:408) binding thiamine diphosphate
D538 (= D433) binding Mg(2+)
DGS 538:540 (= DGS 433:435) binding thiamine diphosphate
N565 (= N460) binding Mg(2+)
NQHLGM 565:570 (≠ NGYLGM 460:465) binding thiamine diphosphate
H567 (≠ Y462) binding Mg(2+)
W574 (= W469) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ A542) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
49% identity, 98% coverage: 2:552/561 of query aligns to 13:578/583 of 5k3sA

query
sites
5k3sA

K

K

G

G

A

A

E

D

A

I

L

L

L

V

K

E

A

A

L

L

E

E

R

R

E

Q

G

G

V

V

E

E

V

T

I

V

F

F

G

A

H
x
Y

P

P

G
|
G

A
|
A

I
x
S

M

M

P

E

T

I

Y

H

D

Q

A

A

L

L

Y

T

-

R

D

S

S

S

P

S

I

I

R

R

H

N

I

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

G

G

I

V

H

F

A

A

T

E

A

G

Y

Y

A

A

R

R

A

S

S

S

G

G

R

K

V

P

G

G

V

I

V

C

M

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

L

T

N

N

L

L

V

V

T

S

G

G

L

L

A

A

D

D

A

A

L

L

M

L

D

D

S

S

T

V

P

P

V

L

V

V

A

A

I

I

T

T

G

G

N

Q

V

V

P

P

R

R

A

R

L

M

I

I

G

G

T

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

V

I

T

V

G

E

V

V

T

T

M

R

P

S

I

I

T

T

K

K

H

H

N

N

Y

Y

L

L

V

V

Q

M

D

D

V

V

N

E

E

D

I

I

P

P

R

R

V

I

K

E

E

E

A

A

F

F

H

F

I

L

A

A

S

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

I

V

D

D

L

V

P

P

K
|
K

D

D

V

I

Q

Q

L

Q

A

Q

E

L

F

A

T

I

G

P

E

N

F

W

D

E

V

Q

E

A

L

M

D

R

L

L

P

P

G

G

Y

Y

-

M

-

S

-

R

-

M

-

P

K

K

P

P

T

P

L

E

R

D

G

S

H

H

P

L

K

E

Q

Q

I

I

E

V

R

R

A

-

L

-

D

-

A

L

L

I

E

S

K

E

A

S

E

K

R

K

P

P

I

V

L

L

M

Y

V

V

G
|
G

A

C

Q

L

H

N

A

S

H

S

A

D

E

E

L

L

L

G

A

R

F

F

A

V

E

E

K

L

T

T

G

G

L

I

P

P

V

V

I

A

T

S

T
|
T

L
|
L

M
|
M

G

G

L

L

G

G

A

S

F

Y

P

P

G

C

N

D

H

D

P

E

L

L

W

S

L

L

G

H

M

M

P
x
L

G

G

M
|
M

H

H

G

G

T

T

V

V

A

Y

A

A

N

N

R

Y

A

A

I

V

H

E

H

H

A

S

D

D

V

L

I

L

L

L

A

A

I

F

G
|
G

L

V

R
|
R

F

F

D
|
D

D

D

R
|
R

V
|
V

T

T

G

G

K

K

V

L

S

E

R

A

F

F

A

A

P

S

H

R

A

A

H

K

T

-

I

I

I

V

H

H

V

I

D
|
D

I
|
I

D

D

P

S

A

A

E

E

I

I

G

G

K

K

V

N

V

K

R

T

T

P

H

H

I

V

P

S

I

V

V

C

G

G

D
|
D

S
x
V

R

K

L

L

V

A

L

L

K

Q

E

G

M

M

L

N

K

K

-

V

-

L

-

E

-

N

G

R

A

A

K

E

P

E

L

L

R

K

L

L

-

D

-

F

A

G

A

V

W

W

W

R

R

N

E

E

L

L

E

N

E

V

W

Q

R

K

T

Q

R

K

Y

F

P

P

L

L

R

S

Y

F

R

K

P

T

R

F

P

G

H

E

L

A

Q

I

A

P

P

P

E

Q

-

Y

V

A

I

I

R

K

A

V

F

L

H

D

E

E

A

L

T

T

G

D

G

G

N

K

A

A

I

I

V

I

T

S

T

T

G

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

F

W

A

A

Q

Q

Y

F

F

Y

T

N

V

Y

T

K

R

K

P

P

R

R

S

Q

F

W

L

L

T

S

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

V

F

G

G

L

L

P

P

F

A

A

A

I

I

G

G

A

A

Q

S

I

V

A

A

R

N

P

P

E

D

E

A

L

I

V

V

I

V

D

D

F

I

D

D

G

G

D
|
D

G
|
G

S
|
S

F

F

Q

I

M

M

T

N

L

V

Q

Q

E

E

L

L

A

A

T

T

V

I

V

R

K

V

Y

E

R

N

L

L

P

P

V

V

K

K

V

V

V

L

I

L

L

L

N

N

N
|
N

G

Q

Y
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

D

D

L

R

F

F

H

Y

A

K

K

A

R

N

Y

R

S

A

E

H

V

T

Y

F

L

L

A

G

D

D

S

P

N

A

-

Q

-

E

-

D

-

E

-

I

-

F

P

P

D

N

F

M

A

L

K

L

L

F

A

A

E

A

A

A

Y

C

G

G

I

I

K

P

G

A

V

A

R

R

V

V

E

T

R

K

K

K

E

A

D

D

L

L

K

R

K

E

G

A

V

I

E

Q

A

T

V

M

L

L

N

D

A

T

D

P

G

G

P

P

V

Y

V

L

A

L

E

D

F

V

K

I

V

C

Y

P

H
|
H

E

Q

E

E

G

H

V

V

F

L

P

P

M

M

I

I

P

P

A

S

G
|
G

G

G

A

T

A

F

E

N

D

D

M

V

I

I

L

T

E

E

active site: Y33 (≠ H22), G35 (= G24), G36 (= G25), A37 (= A26), S38 (≠ I27), E59 (= E47), T82 (= T70), F121 (= F109), Q122 (= Q110), E123 (= E111), K171 (= K159), M266 (= M252), V293 (= V279), V400 (= V380), G426 (= G406), M428 (= M408), D453 (= D433), N480 (= N460), H482 (≠ Y462), L483 (= L463), M485 (= M465), V486 (= V466), W489 (= W469), H558 (= H532)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R278), M485 (= M465), W489 (= W469), G569 (= G543)
binding flavin-adenine dinucleotide: R161 (= R149), G222 (= G208), G223 (= G209), G224 (= G210), T246 (= T232), L247 (= L233), M248 (= M234), L264 (≠ P250), M266 (= M252), G286 (= G272), R288 (= R274), D290 (= D276), V293 (= V279), D310 (= D297), I311 (= I298), D329 (= D316), V330 (≠ S317), M405 (= M385), G423 (= G403)
binding magnesium ion: D453 (= D433), N480 (= N460), H482 (≠ Y462)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V380), G401 (= G381), Q402 (= Q382), H403 (= H383), G426 (= G406), M428 (= M408), D453 (= D433), G454 (= G434), S455 (= S435), N480 (= N460), H482 (≠ Y462), L483 (= L463), G484 (= G464), M485 (= M465), V486 (= V466)

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
49% identity, 98% coverage: 2:552/561 of query aligns to 13:578/582 of 8et4A

query
sites
8et4A

K

K

G

G

A

A

E

D

A

I

L

L

L

V

K

E

A

A

L

L

E

E

R

R

E

Q

G

G

V

V

E

E

V

T

I

V

F

F

G

A

H

Y

P

P

G

G

A

A

I

S

M

M

P

E

T

I

Y

H

D

Q

A

A

L

L

Y

T

-

R

D

S

S

S

P

S

I

I

R

R

H

N

I

V

L

L

V

P

R

R

H

H

E

E

Q

Q

G

G

I

V

H

F

A

A

T

E

A

G

Y

Y

A

A

R

R

A

S

S

S

G

G

R

K

V

P

G

G

V

I

V

C

M

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

L

T

N

N

L

L

V

V

T

S

G

G

L

L

A

A

D

D

A

A

L

L

M

L

D

D

S

S

T

V

P

P

V

L

V

V

A

A

I

I

T

T

G

G

N

Q

V

V

P

P

R

R

A

R

L

M

I

I

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binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V380), G401 (= G381), Q402 (= Q382), H403 (= H383), G426 (= G406), M428 (= M408), G452 (= G432), D453 (= D433), G454 (= G434), S455 (= S435), M458 (= M438), N480 (= N460), H482 (≠ Y462), L483 (= L463), G484 (= G464), M485 (= M465), V486 (= V466)
binding flavin-adenine dinucleotide: R161 (= R149), G222 (= G208), G223 (= G209), G224 (= G210), T246 (= T232), L247 (= L233), M248 (= M234), L264 (≠ P250), M266 (= M252), H267 (= H253), G286 (= G272), V287 (≠ L273), R288 (= R274), D290 (= D276), R292 (= R278), V293 (= V279), D310 (= D297), I311 (= I298), D329 (= D316), V330 (≠ S317), M405 (= M385), G423 (= G403)
binding magnesium ion: F370 (≠ Y351), D453 (= D433), M458 (= M438), Q461 (= Q441), N480 (= N460), H482 (≠ Y462), K533 (= K507)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M252), R292 (= R278), M485 (= M465), W489 (= W469), S568 (≠ A542)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
49% identity, 98% coverage: 2:552/561 of query aligns to 13:578/582 of 5wj1A

query
sites
5wj1A

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&nb