SitesBLAST

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
25% identity, 84% coverage: 11:371/432 of query aligns to 64:424/495 of Q9NRA2

query
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Q9NRA2

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K136 (= K75) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ V120) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AI 135:136) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (= IL 202:203) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ AGQEA 204:208) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (≠ A269) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P275) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (= G312) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
25% identity, 83% coverage: 11:370/432 of query aligns to 64:440/495 of Q8BN82

query
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Q8BN82

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H183 (≠ V120) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
26% identity, 57% coverage: 101:346/432 of query aligns to 164:411/495 of Q5Q0U0

query
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Q5Q0U0

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R168 (= R105) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ L108) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G109) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E112) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A113) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (≠ I116) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P117) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ V120) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ T123) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ AGQEA 204:208) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P275) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
G371 (= G312) mutation to V: Remains in the endoplasmic reticulum.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
136 K→E: Markedly decreases H(+)-coupled sialic acid transporter activity.

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
26% identity, 59% coverage: 74:329/432 of query aligns to 117:366/493 of Q03567

query
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Q03567

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M

F

A

D

G

G

S

G

W

W

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P

W

S

A

I

F

F

V

Y

I

I

V

I

G

G

V

V

L

F

G

G

G

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I

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T

T

A

A

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-

W

W

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D

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T

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A

E

E

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R

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D

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Y

Y

I

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A

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A

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A

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A

S

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P

G

Y

Y

Sites not aligning to the query:

69 modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
28% identity, 53% coverage: 101:329/432 of query aligns to 166:387/497 of Q9Y2C5

query
sites
Q9Y2C5

L

L

A

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-

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A

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L

L

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S

L

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Y

W

A372 (= A313) to T: in dbSNP:rs11754288

Sites not aligning to the query:

66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
24% identity, 49% coverage: 100:312/432 of query aligns to 120:334/452 of 7t3nA

query
sites
7t3nA

G

G

L

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A

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G

binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y137 (≠ I116), Y165 (≠ S144), R264 (≠ S242), F268 (≠ W246), Y269 (≠ Q247)
binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: E152 (= E131), G163 (= G142)

Sites not aligning to the query:

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 49% coverage: 100:312/432 of query aligns to 178:392/582 of Q9JI12

query
sites
Q9JI12

G

G

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x
R

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P

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A

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R184 (= R105) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E112) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (≠ S242) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

Sites not aligning to the query:

88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
128 H→A: Greatly lowers L-glutamate transport.

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
22% identity, 98% coverage: 6:430/432 of query aligns to 17:446/452 of Q5EXK5

query
sites
Q5EXK5

G

G

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A

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M

-

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F

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A

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A

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A

G

G

A

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x
V

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D

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K

-

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A

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T

I

L

V

F

G

T

S

L

L

F

S

F

G

G

G

V

V

L

M

V

M

T

A

-

M

Q

N

V

F

G

S

Y

F

S

D

P

T

L

L

F

F

V

F

M

V

L

I

A

A

I

V

F

P

D

A

V

V

I

V

A

S

A

A

I

V

T

M

L

L

C

I

L

L

A

T

R

L

E

A

R

V

T

R

T

N

P

P

P

T

A

S

L

V

P

P

L

A

A

A

E

L

P

P

A

S

D82 (= D70) mutation to A: Loss of activity.
V311 (≠ Y288) mutation to W: Loss of activity.
D314 (≠ P291) mutation to A: Loss of activity.

A2SWM2 Sphingosine-1-phosphate transporter SPNS2; Protein spinster homolog 2; Protein two of hearts from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
20% identity, 80% coverage: 13:357/432 of query aligns to 59:400/504 of A2SWM2

query
sites
A2SWM2

G

G

M

I

V

L

T

S

A

F

G

G

L

N

I

V

V

L

N

N

Y

Y

L

M

A

D

R

R

N

Y

T

T

L

V

S

A

V

G

A

V

A

L

P

L

T

D

M

I

M

Q

H

K

D

Q

L

F

H

K

M

V

T

G

T

D

K

S

E

S

Y

A

S

G

Y

L

V

L

V

Q

V

T

A

V

W

F

Q

I

A

C

C

S

Y

F

A

M

L

V

M

A

Q

A

P

P

V

I

A

F

G

G

Y

Y

I

L

L

G

D

D

A

R

I

F

G

N

T

R

K

K

I

I

G

-

F

-

A

-

I

I

F

L

A

S

C

C

A

G

-

I

-

F

W

W

S

S

V

A

A

V

C

T

A

L

A

S

F

F

A

I

G

T

N

K

-

E

-

Y

W

Y

Q

W

G

L

L

L

A

V

V

L

F

S

R
|
R

G

C

M

L

L

V

G

G

L

I

T

G

E

E

A

S

G

Y

I

S

P

S

A

I

G

S

V

P

K

T

A

I

T

I

T

G

E

D

W

L

F

F

P

T

A

N

R

N

E

K

R

R

S

T

V

V

A

M

I

L

G

S

W

V

F

F

N

Y

I

L

-

A

-

I

-

P

-

L

G

G

S

S

S

G

I

L

G

G

A

Y

L

I

L

L

A

G

P

S

P

-

L

-

V

-

V

I

W

A

A

K

I

D

M

A

A

G

G

G

S

H

W

W

E

Y

W

W

A

A

F

L

V

R

I

V

V

S

G

P

V

M

L

L

G

G

G

L

I

T

W

A

T

G

A

T

L

L

W

I

M

L

L

I

F

F

Y

V

K

S

H

E

P

P

K

K

N

R

Q

G

K

S

A

A

L

-

S

-

D

-

A

-

E

-

R

-

D

-

Y

-

I

-

L

-

A

-

G

-

Q

-

E

-

A

-

Q

-

Y

-

R

D

E

Q

E

P

G

G

A

G

A

R

V

L

A

K

P

T

R

R

A

T

R

S

W

W

T

V

-

C

-

D

-

M

-

K

A

A

I

L

V

A

K

K

S

N

R

R

N

S

F

Y

R

V

A

F

I

S

A

S

I

L

P

A

R

S

F

A

L

A

S

V

E

S

P

F

A

A

W

T

Q

G

T

A

F

F

N

G

A

I

W

W

I

I

P

P

L

Q

Y

Y

M

L

-

V

-

R

-

A

-

Q

-

V

-

Q

-

K

-

S

-

A

-

E

-

S

A

C

T

T

E

Y

R

Q

H

P

M

C

N

S

L

S

K

R

E

D

I

S

A

L

M

I

F

F

A

G

W

A

L

I

P

T

F

C

L

V

A

T

A

G

D

L

M

L

G

G

C

V

V

V

L

I

G

G

G

A

Y

V

L

T

S

T

P

-