SitesBLAST
Comparing WP_053937127.1 NCBI__GCF_001294205.1:WP_053937127.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q9A3Q9 Omega-aminotransferase; Beta-alanine--pyruvate aminotransferase; EC 2.6.1.-; EC 2.6.1.18 from Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15) (Caulobacter crescentus) (see paper)
53% identity, 98% coverage: 6:444/447 of query aligns to 3:438/439 of Q9A3Q9
- V227 (= V233) mutation to G: Decreases activity toward 3-aminobutanoate by 2-fold. Increases activity toward the aromatic beta-amino acid 3-amino-3-phenylpropanoate by 2-fold.
- R260 (= R266) mutation to L: Decreases activity toward 3-aminobutanoate by 30-fold.
- N285 (= N291) mutation to A: Decreases activity toward 3-aminobutanoate by 4-fold. Increases activity toward the aromatic beta-amino acid 3-amino-3-phenylpropanoate by 3-fold.
Q9I700 Beta-alanine--pyruvate aminotransferase; Beta-A--Py AT; Beta-alanine--pyruvate transaminase; Omega-amino acid aminotransferase; Omega-amino acid AT; Omega-amino acid--pyruvate aminotransferase; Omega-APT; EC 2.6.1.18 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
51% identity, 95% coverage: 10:433/447 of query aligns to 15:436/448 of Q9I700
- W61 (= W58) binding substrate
- T327 (= T324) binding pyridoxal 5'-phosphate
- R414 (= R411) binding substrate
- Q421 (≠ A418) binding substrate
4b98A The structure of the omega aminotransferase from pseudomonas aeruginosa (see paper)
51% identity, 95% coverage: 10:433/447 of query aligns to 8:429/441 of 4b98A
- active site: F17 (= F19), Y146 (= Y150), E219 (= E225), D252 (= D258), I255 (= I261), K281 (= K287), Q414 (≠ A418)
- binding pyridoxal-5'-phosphate: G233 (= G239), Q236 (= Q242), F270 (= F276), G271 (= G277)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: L53 (= L57), W54 (= W58), F82 (= F86), S112 (= S116), G113 (= G117), S114 (= S118), Y146 (= Y150), H147 (= H151), G148 (= G152), E219 (= E225), S224 (= S230), D252 (= D258), V254 (= V260), I255 (= I261), K281 (= K287), Y319 (= Y323), T320 (= T324), R407 (= R411), Q414 (≠ A418)
4uhmA Characterization of a novel transaminase from pseudomonas sp. Strain aac (see paper)
48% identity, 97% coverage: 10:444/447 of query aligns to 2:434/435 of 4uhmA
- active site: F11 (= F19), Y140 (= Y150), E213 (= E225), D246 (= D258), I249 (= I261), K275 (= K287), Q408 (≠ A418)
- binding magnesium ion: A91 (= A101), D99 (= D109)
- binding pyridoxal-5'-phosphate: G107 (= G117), S108 (= S118), Y140 (= Y150), H141 (= H151), G142 (= G152), E213 (= E225), D246 (= D258), V248 (= V260), I249 (= I261), K275 (= K287)
3a8uX Crystal structure of omega-amino acid:pyruvate aminotransferase
48% identity, 97% coverage: 10:444/447 of query aligns to 7:440/441 of 3a8uX
- active site: Y145 (= Y150), D252 (= D258), K281 (= K287), Q414 (≠ A418)
- binding pyridoxal-5'-phosphate: S111 (= S116), G112 (= G117), S113 (= S118), Y145 (= Y150), H146 (= H151), G147 (= G152), E219 (= E225), D252 (= D258), V254 (= V260), I255 (= I261), K281 (= K287)
6gwiB The crystal structure of halomonas elongata amino-transferase (see paper)
34% identity, 97% coverage: 4:438/447 of query aligns to 3:439/450 of 6gwiB
- active site: F18 (= F19), Y149 (= Y150), D255 (= D258), K284 (= K287)
- binding pyridoxal-5'-phosphate: S115 (= S116), G116 (= G117), S117 (= S118), Y149 (= Y150), H150 (= H151), G151 (= G152), E222 (= E225), D255 (= D258), V257 (= V260), I258 (= I261), K284 (= K287)
8wqjA Crystal structure of transaminase from shimia marina
33% identity, 93% coverage: 18:433/447 of query aligns to 23:439/472 of 8wqjA
- binding pyridoxal-5'-phosphate: S121 (= S116), G122 (= G117), S123 (= S118), Y155 (= Y150), H156 (= H151), G157 (= G152), E228 (= E225), D261 (= D258), V263 (= V260), I264 (= I261), K290 (= K287), Y321 (= Y323), T322 (= T324)
6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
36% identity, 91% coverage: 33:441/447 of query aligns to 33:447/448 of 6io1B
- active site: Y151 (= Y150), D257 (= D258), K286 (= K287)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (≠ S116), G118 (= G117), A119 (≠ S118), N122 (≠ I121), Y151 (= Y150), H152 (= H151), D257 (= D258), V259 (= V260), I260 (= I261), K286 (= K287)
Sites not aligning to the query:
3i5tA Crystal structure of aminotransferase prk07036 from rhodobacter sphaeroides kd131
33% identity, 93% coverage: 30:444/447 of query aligns to 24:437/444 of 3i5tA
- active site: Y144 (= Y150), E216 (= E225), D249 (= D258), V252 (≠ I261), K279 (= K287), V411 (≠ A418)
- binding pyridoxal-5'-phosphate: G111 (= G117), S112 (= S118), Y144 (= Y150), H145 (= H151), E216 (= E225), D249 (= D258), V251 (= V260), K279 (= K287), Y315 (= Y323), T316 (= T324)
Sites not aligning to the query:
7qx3A Structure of the transaminase tr2e2 with eos (see paper)
33% identity, 91% coverage: 32:438/447 of query aligns to 2:411/422 of 7qx3A
7ypmA Crystal structure of transaminase cc1012 complexed with plp and l- alanine (see paper)
31% identity, 93% coverage: 22:438/447 of query aligns to 21:441/454 of 7ypmA