SitesBLAST
Comparing WP_053938621.1 NCBI__GCF_001294205.1:WP_053938621.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 10 hits to proteins with known functional sites (download)
5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
25% identity, 88% coverage: 20:394/428 of query aligns to 8:374/383 of 5i39A
- active site: F66 (≠ N73), Q69 (= Q76), A70 (≠ V77), Q248 (vs. gap), P267 (= P277)
- binding flavin-adenine dinucleotide: V30 (= V37), G31 (= G38), G33 (= G40), I34 (≠ L41), L35 (≠ T42), V53 (≠ L60), E54 (= E61), K55 (≠ A62), Q62 (≠ A69), S63 (= S70), F66 (≠ N73), Y67 (≠ G74), Q69 (= Q76), A196 (≠ L211), A197 (≠ V212), G226 (≠ C239), G227 (≠ N240), W229 (≠ F242), Q248 (vs. gap), Q250 (vs. gap), G321 (= G342), M323 (≠ V344), T348 (≠ S368), G349 (= G369), W350 (≠ H370), G351 (= G371), M352 (≠ V372), T353 (≠ N373)
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
25% identity, 84% coverage: 33:391/428 of query aligns to 3:349/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (≠ V37), G8 (= G38), G10 (= G40), V11 (≠ L41), I12 (≠ T42), V30 (≠ L60), E31 (= E61), K32 (≠ A62), E38 (≠ A68), A39 (= A69), S40 (= S70), A43 (≠ N73), G45 (= G75), L46 (≠ Q76), V171 (= V212), G200 (≠ C239), G201 (≠ N240), W203 (≠ F242), G298 (= G342), R300 (≠ V344), P301 (≠ D345), Y326 (≠ S368), R327 (≠ G369), N328 (≠ H370), G329 (= G371), I330 (≠ V372)
5hxwA L-amino acid deaminase from proteus vulgaris (see paper)
27% identity, 58% coverage: 22:269/428 of query aligns to 2:252/433 of 5hxwA
- active site: F58 (≠ N73), Q61 (= Q76), A62 (≠ V77), Q240 (vs. gap)
- binding flavin-adenine dinucleotide: V22 (= V37), G23 (= G38), G25 (= G40), I26 (≠ L41), L27 (≠ T42), E46 (= E61), K47 (≠ A62), E53 (≠ A68), Q54 (≠ A69), S55 (= S70), R57 (= R72), F58 (≠ N73), Y59 (≠ G74), G60 (= G75), Q61 (= Q76), A188 (≠ L211), A189 (≠ V212), G218 (≠ C239), G219 (≠ N240), W221 (≠ F242), Q240 (vs. gap), Q242 (vs. gap)
Sites not aligning to the query:
- active site: 284, 288
- binding cetyl-trimethyl-ammonium: 291, 294, 310, 311, 317, 318, 320, 373, 379, 399, 400
- binding flavin-adenine dinucleotide: 331, 371, 373, 398, 399, 400, 401, 402, 403
3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
23% identity, 75% coverage: 70:389/428 of query aligns to 35:349/364 of 3if9A
- active site: A47 (≠ C83), G48 (= G84), M49 (= M85)
- binding flavin-adenine dinucleotide: S35 (= S70), T42 (≠ V77), T43 (≠ I78), A46 (= A82), A47 (≠ C83), G48 (= G84), M49 (= M85), P173 (≠ L211), V174 (= V212), S202 (≠ C239), G203 (≠ N240), W205 (vs. gap), F209 (= F242), G300 (= G342), R302 (≠ V344), H327 (≠ F367), F328 (≠ S368), R329 (≠ G369), N330 (≠ H370), G331 (= G371), I332 (≠ V372)
- binding glycolic acid: Y246 (= Y291), R302 (≠ V344), R329 (≠ G369)
Sites not aligning to the query:
1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
21% identity, 80% coverage: 47:387/428 of query aligns to 20:352/374 of 1y56B
- active site: F44 (= F81), G47 (= G84), T48 (≠ M85), H224 (≠ I262), P239 (= P277), G305 (= G342), M338 (≠ N373)
- binding flavin-adenine dinucleotide: I33 (≠ L60), E34 (= E61), K35 (≠ A62), S42 (≠ A69), T43 (≠ S70), R45 (≠ A82), C46 (= C83), G47 (= G84), G49 (≠ D86), E170 (≠ L211), V171 (= V212), T200 (≠ C239), N201 (= N240), W203 (≠ F242), G305 (= G342), Y306 (≠ M343), Y307 (≠ V344), G334 (= G369), H335 (= H370), G336 (= G371), F337 (≠ V372), M338 (≠ N373)
- binding flavin mononucleotide: F44 (= F81), R45 (≠ A82), I260 (≠ D297), R301 (≠ H338), W303 (= W340)
Sites not aligning to the query:
1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
23% identity, 57% coverage: 157:400/428 of query aligns to 117:360/364 of 1ng3A
- binding acetylamino-acetic acid: Y246 (= Y291), R302 (≠ V344), R329 (≠ G369)
- binding flavin-adenine dinucleotide: V174 (= V212), S202 (≠ C239), G203 (≠ N240), W205 (vs. gap), F209 (= F242), G300 (= G342), R302 (≠ V344), H327 (≠ F367), R329 (≠ G369), N330 (≠ H370), G331 (= G371), I332 (≠ V372)
- binding phosphate ion: R254 (= R299)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49
- binding phosphate ion: 89
O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
23% identity, 54% coverage: 157:389/428 of query aligns to 117:349/369 of O31616
- V174 (= V212) binding FAD
- H244 (≠ R286) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
- R302 (≠ V344) binding substrate
- 327:333 (vs. 367:373, 14% identical) binding FAD
- R329 (≠ G369) binding substrate
Sites not aligning to the query:
- 14:15 binding FAD
- 34:35 binding FAD
- 42:43 binding FAD
- 47:49 binding FAD
- 51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
- 54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
5fjnA Structure of l-amino acid deaminase from proteus myxofaciens in complex with anthranilate (see paper)
29% identity, 48% coverage: 32:238/428 of query aligns to 29:229/447 of 5fjnA
- active site: S67 (= S70), Y71 (≠ G74), S72 (≠ G75)
- binding flavin-adenine dinucleotide: I34 (≠ V37), G35 (= G38), G37 (= G40), I38 (≠ L41), Q39 (≠ T42), L57 (= L60), E58 (= E61), K59 (≠ A62), E65 (≠ A68), Q66 (≠ A69), S67 (= S70), A70 (≠ N73), Y71 (≠ G74), S72 (≠ G75), Q73 (= Q76), V201 (= V212)
Sites not aligning to the query:
- active site: 252
- binding 2-aminobenzoic acid: 252, 289, 411, 412
- binding flavin-adenine dinucleotide: 230, 231, 233, 252, 254, 343, 385, 410, 411, 412, 413, 414, 415
5fjmA Structure of l-amino acid deaminase from proteus myxofaciens (see paper)
29% identity, 48% coverage: 32:238/428 of query aligns to 29:229/447 of 5fjmA
- active site: S67 (= S70), Y71 (≠ G74), S72 (≠ G75)
- binding flavin-adenine dinucleotide: I34 (≠ V37), G35 (= G38), G37 (= G40), I38 (≠ L41), Q39 (≠ T42), L57 (= L60), E58 (= E61), K59 (≠ A62), E65 (≠ A68), Q66 (≠ A69), S67 (= S70), A70 (≠ N73), Y71 (≠ G74), S72 (≠ G75), Q73 (= Q76), V201 (= V212)
Sites not aligning to the query:
- active site: 252
- binding flavin-adenine dinucleotide: 230, 231, 233, 252, 254, 343, 385, 410, 411, 412, 413, 414, 415
2gagB Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
21% identity, 83% coverage: 33:387/428 of query aligns to 21:371/403 of 2gagB
- active site: A61 (= A82), T64 (≠ M85), T65 (≠ D86)
- binding flavin-adenine dinucleotide: G26 (= G38), G28 (= G40), G29 (≠ L41), H30 (≠ T42), E51 (≠ R64), K52 (≠ V65), G58 (= G71), N59 (≠ R72), M60 (≠ F81), R62 (≠ C83), N63 (≠ G84), T64 (≠ M85), I66 (≠ T87), V195 (= V212), G224 (≠ C239), A225 (≠ N240), H227 (≠ F242), L231 (= L246), L246 (≠ I267), G352 (≠ S368), T353 (≠ G369), G354 (≠ H370), G355 (= G371), F356 (≠ V372), K357 (≠ N373)
- binding flavin mononucleotide: A61 (= A82), R62 (≠ C83), H171 (≠ P188), V250 (≠ R271), E278 (≠ R299), R321 (≠ H338), W323 (= W340)
- binding 2-furoic acid: T64 (≠ M85), I66 (≠ T87), R68 (≠ E89), M263 (≠ D284), Y270 (= Y291), K357 (≠ N373)
- binding sulfite ion: K170 (≠ H187), K276 (≠ D297)
Sites not aligning to the query:
Query Sequence
>WP_053938621.1 NCBI__GCF_001294205.1:WP_053938621.1
MHIEQTEHIASWYAATANDSTRHAPLMGTLACDVLVVGAGLTGLHAALNLAERGYKVVVL
EAARVGWAASGRNGGQVINGFACGMDTFESELPKEDLQRVFDMGIEANTLLRERIAKHNI
DCDYRAGYLTAANKAAHVKDLQAWRDEAAQRWGYPDLSWVERSELPGYVQSERYAGGLFD
PNSGHLHPLNYTLGLARAAVAAGVQIFEHSLVTEMQQGTPHVLKTAHGQVQAQFVVLACN
AFIEHLSPALNRKIMPVGTYVITTEPITPVRFKALMPAGAAICDSRFVLDYFRPTPDGRV
LWGGKVSYSTLAPANLAQAMRADMLKTFPQLADIKIIHAWGGMVDITMNRAPHFGRLAST
VYFAQGFSGHGVNVSGLAGKLMAEAIAGQAERFDIFTKIRHHDFPGGDLLRTPSLVLAMA
WYRLKDLV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory