SitesBLAST

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
25% identity, 87% coverage: 39:413/433 of query aligns to 102:478/495 of Q9NRA2

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K136 (= K73) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ N118) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AM 133:134) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
I----L 266:267 (≠ IETLKA 201:206) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ SDPAT 207:211) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (≠ S267) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P273) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (≠ T308) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
26% identity, 87% coverage: 39:413/433 of query aligns to 102:478/495 of Q5Q0U0

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K136 (= K73) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
R168 (= R103) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ F106) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G107) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E110) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (= G111) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (≠ C114) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (≠ S115) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ N118) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ I121) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ ETLK- 202:205) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P273) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
G371 (≠ T308) mutation to V: Remains in the endoplasmic reticulum.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
23% identity, 92% coverage: 1:398/433 of query aligns to 10:443/493 of Q03567

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C

A

G

M

Q

M

D

S

V

V

L

S

V

I

I

F

I

F

F

-

I

-

T

-

C

-

G

L

M

Y

A

V

I

T

S

G

G

S

L

I

Q

Y

Y

W

A

A

G

I

F

I

V

Q

V

D

N

V

Y

V

L

H

E

-

I

-

A

P

P

A

P

R

F

V

S

G

G

S

T

V

V

S

M

G

G

F

T

V

G

H

N

L

T

V

I

G

S

S

A

V

L

S

A

G

G

I

I

V

I

G

S

P

P

I

A

V

V

T

S

G

S

T

Y

I

L

V

T

Q

P

N

N

T

G

G

T

K

Q

F

E

D

E

S

W

A

Q

F

M

I

V

L

L

N69 (≠ H36) modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
25% identity, 87% coverage: 39:413/433 of query aligns to 102:478/495 of Q8BN82

query
sites
Q8BN82

Q

K

L

W

N

D

A

A

Q

E

Q

T

F

Q

G

G

V

W

I

I

F

L

G

G

S

S

F

F

F

Y

G

G

Y

Y

A

I

I

V

F

T

N

Q

F

I

I

P

G

G

L

Y

A

I

V

A

D

S

R

R

F

V

G

G

P

G

K

K

V

L

M

L

G

G

L

L

A

G

V

I

V

L

F

G

W

T

S

S

I

V

F

F

C

T

G

L

L

F

T

T

A

P

V

L

A

A

T

A

-

D

-

L

G

G

F

V

Y

V

S

T

M

L

L

V

I

V

L

L

R

R

V

A

L

L

F

E

G

G

M

L

A

G

E

E

G

G

P

V

I

T

C

F

S

P

S

A

A

M

N
x
H

K

A

M

M

I

W

N

S

G

S

W

W

F

A

P

P

R

P

R

L

R

E

A

R

A

S

T

K

A

L

M

L

G

T

I

I

L

S

S

Y

A

A

G

G

S

A

P

Q

L

L

G

G

G

T

A

V

V

I

A

S

G

L

P

P

I

L

V

S

G

G

A

I

L

I

A

C

V

Y

A

Y

L

M

G

N

W

W

R

T

Q

Y

A

V

F

F

V

Y

V

L

I

F

C

G

A

I

I

V

G

G

L

I

V

V

W

W

A

F

V

I

I

L

W

W

F

M

I

W

V

I

A

V

A

S

N

D

K

T

P

P

A

E

E

T

S

H

K

K

Y

T

V

I

T

S

A

H

R

Y

E

E

R

K

E

E

-

Y

L

I

I

V

E

S

T

S

L

L

K

K

-

N

-

Q

-

L

A

S

S

S

D

Q

P

K

A

V

T

V

P

P

Q

W

A

G

T

S

S

-

A

-

S

-

D

-

G

-

L

-

H

-

Y

I

L

L

K

K

Q

S

P

L

V

P

I

L

M

W

A

A

T

I

A

V

F

V

A

A

F

H

F

F

C

S

Y

Y

N

N

Y

W

I

S

L

F

F

Y

F

T

F

L

L

L

S

T

W

L

F

L

P

P

T

T

Y

Y

L

M

V

K

Q

E

A

I

H

L

G

R

L

F

N

N

I

V

K

Q

Q

E

M

N

S

G

L

F

T

L

T

S

V

A

I

L

P

P

W

Y

V

F

V

G

G

C

F

W

V

L

G

C

L

M

A

I

L

L

G

C

G

G

V

Q

I

A

S

A

D

D

A

Y

I

L

-

R

L

V

R

K

W

W

T

N

G

F

K

S

S

T

L

I

L

S

S

V

R

R

K

R

I

I

V

F

-

S

L

L

T

V

T

G

C

M

L

V

G

G

A

P

A

A

A

V

L

F

C

L

V

V

A

A

L

A

A

G

G

F

N

I

I

G

A

C

S

D

V

Y

V

S

P

L

A

A

V

V

A

A

M

F

M

L

S

T

V

I

S

S

I

T

F

T

F

L

L

G

Y

G

V

F

T

A

G

S

S

S

I

G

Y

F

W

-

A

S

I

I

I

N

Q

H

D

L

V

D

V

I

H

A

P

P

A

S

R

Y

V

A

G

G

S

I

V

L

S

L

G

G

F

I

V

T

H

N

L

T

V

F

G

A

S

T

V

I

S

P

G

G

I

M

V

T

G

G

P

P

I

I

V

I

T

A

G

K

T

S

I

L

V

T

Q

P

N

D

T

N

G

T

-

I

-

R

K

E

F

W

D

Q

S

T

A

V

F

F

I

C

L

I

A

A

G

A

A

A

V

I

A

N

A

V

T

F

G

G

A

A

I

I

-

F

W

F

V

T

L

L

F

F

A

A

H183 (≠ N118) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
25% identity, 91% coverage: 41:433/433 of query aligns to 120:518/582 of Q9JI12

query
sites
Q9JI12

N

D

A

P

Q

E

Q

T

F

V

G

G

V

M

I

I

F
x
H

G

G

S

S

F

F

F

W

G

G

Y

Y

A

I

I

I

F

T

N

Q

F

I

I

P

G

G

L

Y

A

I

V

A

D

S

R

R

F

L

G

A

P

A

K

N

V

R

V

V

M

F

G

G

L

A

A

A

V

I

V

L

F

L

W

T

S

S

I

T

F

L

C

N

G

M

L

L

-

I

-

P

T

S

A

A

V

A

A

R

T

V

G

H

F

Y

Y

G

S

C

M

V

L

I

I

F

L

V

R
|
R

V

I

L

L

F

Q

G

G

M

L

A

V

E
|
E

G

G

P

V

I

T

C

Y

S

P

S

A

A

C

N

H

K

G

M

I

I

W

N

S

G

K

W

W

F

A

P

P

-

P

-

L

-

E

R

R

R

S

R

R

A

L

A

A

T

T

A

T

M

S

G

-

I

-

L

-

S

F

A

C

G

G

S

S

P

Y

L

A

G

G

G

A

A

V

V

I

A

A

G

M

P

P

I

L

V

A

G

G

A

I

L

L

A

V

V

Q

A

Y

L

T

G

G

W

W

R

S

Q

S

A

V

F

F

V

Y

V

V

I

Y

C

G

A

S

I

F

G

G

L

M

V

V

W

W

A

Y

V

M

I

F

W

W

F

L

I

L

V

V

A

S

A

Y

N

E

K

S

P

P

A

A

E

K

S

H

K

P

Y

T

V

I

T

T

A

D

R

E

E

E

R

R

E

R

L

Y

I

I

E

E

T

-

L

-

K

-

A

-

S

-

D

-

P

-

A

E

T

S

P

I

Q

G

A

E

T

S

S

A

A

N

S

L

D

L

G

G

L

A

L

M

H

E

Y

K

L

F

K

K

Q

T

P

P

-

W

-

R

-

K

-

F

-

T

-

S

-

M

V

P

I

V

M

Y

A

A

T

I

A

I

F

V

A

A

F

N

F

F

C

C

Y
x
R

N

S

Y

W

I

T

L

F

F

Y

F

L

L

L

S

I

W

S

F

Q

P

P

T

A

Y

Y

L

F

V

E

Q

E

A

V

H

F

G

G

L

F

N

E

I

I

K

S

Q

K

M

V

S

G

L

M

T

L

T

S

V

A

I

V

P

P

W

H

V

L

V

V

G

M

F

T

V

I

G

I

L

V

A

P

L

I

G

G

V

Q

I

I

S

A

D

D

A

-

I

F

L

L

R

R

W

-

T

-

G

S

K

K

S

Q

L

I

L

L

S

S

-

T

-

V

R

R

K

K

I

I

V

M

L

N

T

C

T

G

C

G

L

F

G

G

A

M

A

E

A

A

L

T

C

L

V

L

A

L

L

V

A

V

G

G

N

Y

I

S

A

H

S

T

V

R

V

G

P

V

A

A

V

I

A

S

M

F

M

L

S

V

V

L

S

A

I

V

F

G

F

F

L

S

Y

G

V

F

T

A

G

I

S

S

I

G

Y

F

W

N

A

V

I

N

I

H

Q

L

D

D

V

I

V

A

H

-

P

P

A

R

R

Y

V

A

G

S

S

I

V

L

S

M

G

G

F

I

V

S

H

N

L

G

V

V

G

G

S

T

V

L

S

S

G

G

I

M

V

V

G

C

P

P

I

I

V

I

T

V

G

G

T

A

I

M

V

T

Q

K

N

N

T

K

G

S

K

R

-

E

F

W

D

Q

S

Y

A

V

F

F

I

L

L

I

A

A

G

A

A

L

V

V

A

H

A

Y

T

G

G

G

A

V

I

I

-

F

W

Y

V

A

L

L

F

F

A

A

L

S

K

G

D

E

P

K

R

Q

K

P

P

W

E

A

G

D

P

P

K

E

T

E

V

T

D

S

L

E

Q

E

S

K

A

C

A

G

L

F

H128 (≠ F49) mutation to A: Greatly lowers L-glutamate transport.
R184 (= R103) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E110) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (≠ Y240) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

Sites not aligning to the query:

88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
25% identity, 89% coverage: 41:424/433 of query aligns to 62:451/452 of 7t3nA

query
sites
7t3nA

N

D

A

P

Q

E

Q

T

F

V

G

G

V

M

I

I

F

H

G

G

S

S

F

F

F

W

G

G

Y
|
Y

A

I

I

I

F

T

N

Q

F

I

I

P

G

G

L

Y

A

I

V

A

D

S

R

R

F

L

G

A

P

A

K

N

V

R

V

V

M

F

G

G

L

A

A

A

V

I

V

L

F

L

W

T

S

S

I

T

F

L

C

N

G

M

L

L

-

I

-

P

T

S

A

A

V

A

A

R

T

V

G

H

F

Y

Y

G

S

C

M

V

L

I

I

F

L

V

R

Q

V

I

L

L

F

Q

G

G

M

L

A

V

E

Q

G

G

P

V

I

T

C
x
Y

S

P

S

A

A

C

N

H

K

G

M

I

I

W

N

S

G

K

W

W

F

A

P

P

-

P

-

L

-
x
E

R

R

R

S

R

R

A

L

A

A

T

T

A

T

M

S

G

-

I

-

L

-

S

F

A

C

G
|
G

S

S

P
x
Y

L

A

G

G

G

A

A

V

V

I

A

A

G

M

P

P

I

L

V

A

G

G

A

I

L

L

A

V

V

Q

A

Y

L

T

G

G

W

W

R

S

Q

S

A

V

F

F

V

Y

V

V

I

Y

C

G

A

S

I

F

G

G

L

M

V

V

W

W

A

Y

V

M

I

F

W

W

F

L

I

L

V

V

A

S

A

Y

N

E

K

S

P

P

A

A

E

K

S

H

K

P

Y

T

V

I

T

T

A

D

R

E

E

E

R

R

E

R

L

Y

I

I

-

E

E

E

T

S

L

I

K

G

A

E

S

S

D

A

P

N

A

L

T

L

P

G

Q

A

A

M

T

E

S

K

A

F

S

K

D

T

G

P

L

W

L

R

H

K

Y

F

L

F

K

T

Q

S

P

M

V

P

I

V

M

Y

A

A

T

I

A

I

F

V

A

A

F

N

F

F

C

C

Y
x
R

N

S

Y

W

I

T

L
x
F

F
x
Y

F

L

L

L

S

I

W

S

F

Q

P

P

T

A

Y

Y

L

F

V

E

Q

E

A

V

H

F

G

G

L

F

N

E

I

I

K

S

Q

K

M

V

S

G

L

M

T

L

T

S

V

A

I

V

P

P

W

H

V

L

V

V

G

M

F

T

V

I

G

I

L

V

A

P

L

I

G

G

V

Q

I

I

S

A

D

D

A

-

I

F

L

L

R

R

W

-

T

-

G

S

K

K

S

Q

L

I

L

L

S

S

-

T

-

V

R

R

K

K

I

I

V

M

L

N

T

C

T

G

C

G

L

F

G

G

A

M

A

E

A

A

L

T

C

L

V

L

A

L

L

V

A

V

G

G

N

Y

I

S

A

H

S

T

V

R

V

G

P

V

A

A

V

I

A

S

M

F

M

L

S

V

V

L

S

A

I

V

F

G

F

F

L

S

Y

G

V

F

T

A

G

I

S

S

I

G

Y

F

W

N

A

V

I

N

I

H

Q

L

D

D

V

I

V

A

H

-

P

P

A

R

R

Y

V

A

G

S

S

I

V

L

S

M

G

G

F

I

V

S

H

N

L

G

V

V

G

G

S

T

V

L

S

S

G

G

I

M

V

V

G

C

P

P

I

I

V

I

T

V

G

G

T

A

I

M

V

T

Q

K

N

N

T

K

G

S

K

R

-

E

F

W

D

Q

S

Y

A

V

F

F

I

L

L

I

A

A

G

A

A

L

V

V

A

H

A

Y

T

G

G

G

A

V

I

I

-

F

W

Y

V

A

L

L

F

F

A

A

L

S

K

G

D

E

P

K

R

Q

K

P

P

W

E

A

G

D

P

P

K

E

binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y77 (= Y56), Y137 (≠ C114), Y165 (≠ P142), R264 (≠ Y240), F268 (≠ L244), Y269 (≠ F245)
binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: E152 (vs. gap), G163 (= G140)

Sites not aligning to the query:

binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: 12, 13

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
23% identity, 85% coverage: 46:411/433 of query aligns to 111:478/497 of Q9Y2C5

query
sites
Q9Y2C5

G

G

V

I

I

I

F

L

G

S

S

S

F

L

F

N

F

Y

G

G

Y

S

A

F

I

L

F

A

N

P

F

I

I

P

G

S

G

G

L

Y

A

V

V

A

D

G

R

I

F

F

G

G

P

A

K

K

V

Y

V

V

M

V

G

G

L

A

A

G

V

L

V

F

-

I

-

S

F

F

W

L

S

T

I

L

F

F

C

I

G

P

L

L

T

A

A

A

V

N

A

A

T

-

G

G

F

V

Y

A

S

L

M

L

L

I

I

V

L

L

R

R

V

I

L

V

F

Q

G

G

M

I

A

A

E

Q

G

V

P

M

I

V

C

L

S

T

S

G

A

Q

N

Y

K

S

M

I

I

W

N

V

G

K

W

W

F

A

P

P

R

P

R

L

R

E

A

R

A

S

T

Q

A

L

M

T

G

T

I

I

L

A

S

G

A

S

G

G

S

S

P

M

L

L

G

G

G

S

A

F

V

I

A

V

G

L

P

L

I

A

V

G

G

G

A

L

L

L

A

C

V

Q

A

T

L

I

G

G

W

W

R

P

Q

Y

A

V

F

F

V

Y

V

I

I

F

C

G

A

G

I

I

G

G

L

C

V

A

W

C

A

C

V

P

I

L

W

W

F

F

I

P

V

L

A

I

A

Y

N

D

K

D

P

P

A

V

E

N

S

H

K

P

Y

F

V

I

T

S

A

A

R

G

E

E

R

K

E

R

L

Y

I

I

E

V

T

C

L

S

K

L

A

A

S

Q

D

Q

P

D

A

C

T

S

P

P

Q

G

A

W

T

S

S

L

A

P