SitesBLAST
Comparing WP_053939463.1 NCBI__GCF_001294205.1:WP_053939463.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q6BF17 D-galactonate dehydratase; GalD; EC 4.2.1.6 from Escherichia coli (strain K12)
82% identity, 100% coverage: 1:382/382 of query aligns to 1:382/382 of Q6BF17
- H185 (= H185) mutation H->N,Q: Loss of activity.
- H285 (= H285) mutation to N: Loss of activity.
- E310 (= E310) mutation to Q: Loss of activity.
3rraB Crystal structure of enolase prk14017 (target efi-500653) from ralstonia pickettii 12j with magnesium bound
79% identity, 100% coverage: 1:382/382 of query aligns to 2:375/379 of 3rraB
- active site: I35 (≠ L34), R38 (= R37), Y118 (= Y117), K145 (= K144), N147 (= N146), E151 (= E150), D184 (= D183), H186 (= H185), E210 (= E209), G235 (= G234), E236 (= E235), R237 (= R236), Q257 (= Q256), D259 (= D258), H286 (= H285), P288 (= P287), E311 (= E310)
- binding magnesium ion: D184 (= D183), E210 (= E209), E236 (= E235)
3gy1B Crystal structure of putative mandelate racemase/muconate lactonizing protein from clostridium beijerinckii ncimb 8052
32% identity, 93% coverage: 3:359/382 of query aligns to 7:362/388 of 3gy1B
- active site: R152 (≠ K144), Q154 (≠ N146), D191 (= D183), H193 (= H185), E217 (= E209), G242 (= G234), E243 (= E235), R264 (≠ Q256), H266 (≠ D258), H293 (= H285), E320 (= E310)
- binding magnesium ion: D191 (= D183), E217 (= E209), E243 (= E235)
3p93C Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg,d-mannonate and 2-keto-3-deoxy-d- gluconate
33% identity, 96% coverage: 15:382/382 of query aligns to 17:384/384 of 3p93C
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (vs. gap), D192 (= D183), H194 (= H185), E218 (= E209), G243 (= G234), E244 (= E235), R265 (≠ Q256), P267 (≠ D258), H294 (= H285), G295 (≠ C286), E321 (= E310), W384 (= W382)
- binding 2-keto-3-deoxygluconate: N37 (≠ E35), D192 (= D183), H194 (= H185), E244 (= E235), H294 (= H285), P296 (= P287), D298 (vs. gap), E321 (= E310), W384 (= W382)
- binding magnesium ion: D192 (= D183), E218 (= E209), E244 (= E235)
3p93A Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg,d-mannonate and 2-keto-3-deoxy-d- gluconate
33% identity, 96% coverage: 15:382/382 of query aligns to 17:384/384 of 3p93A
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (vs. gap), D192 (= D183), H194 (= H185), E218 (= E209), G243 (= G234), E244 (= E235), R265 (≠ Q256), P267 (≠ D258), H294 (= H285), G295 (≠ C286), E321 (= E310), W384 (= W382)
- binding d-mannonic acid: N37 (≠ E35), D192 (= D183), H194 (= H185), E244 (= E235), H294 (= H285), P296 (= P287), D298 (vs. gap), E321 (= E310)
- binding magnesium ion: D192 (= D183), E218 (= E209), E244 (= E235)
3rgtA Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with d-arabinohydroxamate
33% identity, 96% coverage: 15:382/382 of query aligns to 17:383/383 of 3rgtA
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (≠ N146), D191 (= D183), H193 (= H185), E217 (= E209), G242 (= G234), E243 (= E235), R264 (≠ Q256), P266 (≠ D258), H293 (= H285), G294 (≠ C286), E320 (= E310), W383 (= W382)
- binding cobalt (ii) ion: D191 (= D183), E217 (= E209), E243 (= E235)
- binding (2S,3R,4R)-2,3,4,5-tetrahydroxy-N-oxo-pentanamide: N37 (≠ E35), D191 (= D183), H193 (= H185), E243 (= E235), H293 (= H285), P295 (= P287), D297 (vs. gap), E320 (= E310), W383 (= W382)
4e4fB Crystal structure of enolase pc1_0802 (target efi-502240) from pectobacterium carotovorum subsp. Carotovorum pc1
32% identity, 100% coverage: 1:382/382 of query aligns to 2:381/381 of 4e4fB
- active site: L37 (= L34), R40 (= R37), R148 (≠ K144), Q150 (≠ N146), D189 (= D183), H191 (= H185), E215 (= E209), G240 (= G234), E241 (= E235), V242 (≠ R236), R262 (≠ Q256), T264 (≠ D258), H291 (= H285), P293 (= P287), E318 (= E310), W381 (= W382)
- binding magnesium ion: D189 (= D183), E215 (= E209), E241 (= E235)
A6M2W4 D-galactonate dehydratase family member Cbei_4837 from Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum) (see paper)
31% identity, 93% coverage: 3:359/382 of query aligns to 6:376/399 of A6M2W4
- D205 (= D183) binding Mg(2+)
- E231 (= E209) binding Mg(2+)
- E257 (= E235) binding Mg(2+)
4kwsA Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and glycerol
33% identity, 96% coverage: 15:382/382 of query aligns to 17:397/397 of 4kwsA
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (≠ N146), Y159 (vs. gap), D205 (= D183), H207 (= H185), E231 (= E209), G256 (= G234), E257 (= E235), R278 (≠ Q256), P280 (≠ D258), H307 (= H285), G308 (≠ C286), E334 (= E310), W397 (= W382)
- binding magnesium ion: D205 (= D183), E231 (= E209), E257 (= E235)
3qkeA Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and d-gluconate
33% identity, 96% coverage: 15:382/382 of query aligns to 17:397/397 of 3qkeA
- active site: H122 (≠ W119), R147 (≠ K144), Q149 (≠ N146), Y159 (vs. gap), D205 (= D183), H207 (= H185), E231 (= E209), G256 (= G234), E257 (= E235), R278 (≠ Q256), P280 (≠ D258), H307 (= H285), G308 (≠ C286), E334 (= E310), W397 (= W382)
- binding D-gluconic acid: N37 (≠ E35), Y159 (vs. gap), D205 (= D183), H207 (= H185), E257 (= E235), H307 (= H285), P309 (= P287), D311 (vs. gap), E334 (= E310), W397 (= W382)
- binding magnesium ion: D205 (= D183), E231 (= E209), E257 (= E235)
Q8FHC7 D-galactonate dehydratase family member RspA; D-mannonate dehydratase; Starvation sensing protein RspA homolog; EC 4.2.1.-; EC 4.2.1.8 from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) (see paper)
31% identity, 100% coverage: 1:382/382 of query aligns to 12:415/415 of Q8FHC7
- D223 (= D183) binding Mg(2+)
- E249 (= E209) binding Mg(2+)
- D250 (≠ E210) binding Mg(2+)
- E275 (= E235) binding Mg(2+)
4il2B Crystal structure of d-mannonate dehydratase (rspa) from e. Coli cft073 (efi target efi-501585)
31% identity, 100% coverage: 1:382/382 of query aligns to 1:404/404 of 4il2B