SitesBLAST
Comparing WP_053939623.1 NCBI__GCF_001294205.1:WP_053939623.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 7 hits to proteins with known functional sites (download)
5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
24% identity, 86% coverage: 28:394/428 of query aligns to 18:374/383 of 5i39A
- active site: F66 (≠ N74), Q69 (= Q77), A70 (≠ V78), Q248 (= Q271), P267 (≠ D290)
- binding flavin-adenine dinucleotide: V30 (= V38), G31 (= G39), G33 (= G41), I34 (≠ Y42), L35 (≠ S43), V53 (≠ L61), E54 (= E62), K55 (≠ A63), Q62 (≠ A70), S63 (= S71), F66 (≠ N74), Y67 (≠ G75), Q69 (= Q77), A196 (= A211), A197 (≠ V212), G226 (≠ A239), G227 (≠ N240), W229 (≠ Y242), Q248 (= Q271), Q250 (= Q273), G321 (= G342), M323 (= M348), T348 (≠ C368), G349 (= G369), W350 (≠ H370), G351 (= G371), M352 (≠ V372), T353 (≠ A373)
1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
23% identity, 79% coverage: 48:387/428 of query aligns to 20:352/374 of 1y56B
- active site: F44 (≠ G72), G47 (= G75), T48 (≠ G76), H224 (≠ A272), P239 (≠ F287), G305 (= G342), M338 (≠ A373)
- binding flavin-adenine dinucleotide: I33 (≠ L61), E34 (= E62), K35 (≠ A63), S42 (≠ A70), T43 (≠ S71), R45 (= R73), C46 (≠ N74), G47 (= G75), G49 (≠ Q77), E170 (≠ A211), V171 (= V212), T200 (≠ A239), N201 (= N240), W203 (≠ Y242), G305 (= G342), Y306 (≠ L343), Y307 (≠ V344), G334 (= G369), H335 (= H370), G336 (= G371), F337 (≠ V372), M338 (≠ A373)
- binding flavin mononucleotide: F44 (≠ G72), R45 (= R73), I260 (≠ G303), R301 (≠ H338), W303 (= W340)
Sites not aligning to the query:
5hxwA L-amino acid deaminase from proteus vulgaris (see paper)
25% identity, 65% coverage: 28:306/428 of query aligns to 10:278/433 of 5hxwA
- active site: F58 (≠ N74), Q61 (= Q77), A62 (≠ V78), Q240 (= Q271)
- binding flavin-adenine dinucleotide: V22 (= V38), G23 (= G39), G25 (= G41), I26 (≠ Y42), L27 (≠ S43), E46 (= E62), K47 (≠ A63), E53 (≠ G69), Q54 (≠ A70), S55 (= S71), R57 (= R73), F58 (≠ N74), Y59 (≠ G75), G60 (= G76), Q61 (= Q77), A188 (= A211), A189 (≠ V212), G218 (≠ A239), G219 (≠ N240), W221 (≠ Y242), Q240 (= Q271), Q242 (= Q273)
Sites not aligning to the query:
- active site: 284, 288
- binding cetyl-trimethyl-ammonium: 291, 294, 310, 311, 317, 318, 320, 373, 379, 399, 400
- binding flavin-adenine dinucleotide: 331, 371, 373, 398, 399, 400, 401, 402, 403
5fjnA Structure of l-amino acid deaminase from proteus myxofaciens in complex with anthranilate (see paper)
24% identity, 68% coverage: 26:317/428 of query aligns to 20:301/447 of 5fjnA
- active site: S67 (= S71), Y71 (≠ G75), S72 (≠ G76), L252 (≠ I261)
- binding 2-aminobenzoic acid: L252 (≠ I261), R289 (= R305)
- binding flavin-adenine dinucleotide: I34 (≠ V38), G35 (= G39), G37 (= G41), I38 (≠ Y42), Q39 (≠ S43), L57 (= L61), E58 (= E62), K59 (≠ A63), E65 (≠ G69), Q66 (≠ A70), S67 (= S71), A70 (≠ N74), Y71 (≠ G75), S72 (≠ G76), Q73 (= Q77), V201 (= V212), G230 (≠ A239), G231 (≠ N240), W233 (≠ Y242), L252 (≠ I261), Q254 (= Q273)
Sites not aligning to the query:
5fjmA Structure of l-amino acid deaminase from proteus myxofaciens (see paper)
24% identity, 68% coverage: 26:317/428 of query aligns to 20:301/447 of 5fjmA
- active site: S67 (= S71), Y71 (≠ G75), S72 (≠ G76), L252 (≠ I261)
- binding flavin-adenine dinucleotide: I34 (≠ V38), G35 (= G39), G37 (= G41), I38 (≠ Y42), Q39 (≠ S43), L57 (= L61), E58 (= E62), K59 (≠ A63), E65 (≠ G69), Q66 (≠ A70), S67 (= S71), A70 (≠ N74), Y71 (≠ G75), S72 (≠ G76), Q73 (= Q77), V201 (= V212), G230 (≠ A239), G231 (≠ N240), W233 (≠ Y242), L252 (≠ I261), Q254 (= Q273)
Sites not aligning to the query:
2gagB Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
25% identity, 87% coverage: 34:407/428 of query aligns to 21:390/403 of 2gagB
- active site: A61 (≠ G72), T64 (≠ D86), T65 (= T87)
- binding flavin-adenine dinucleotide: G26 (= G39), G28 (= G41), G29 (≠ Y42), H30 (≠ S43), E51 (= E62), K52 (≠ A63), G58 (= G69), N59 (≠ A70), M60 (≠ S71), R62 (= R73), N63 (= N74), T64 (≠ D86), I66 (≠ V88), V195 (= V212), G224 (≠ A239), A225 (≠ N240), H227 (≠ Y242), L231 (= L247), L246 (= L267), G352 (≠ C368), T353 (≠ G369), G354 (≠ H370), G355 (= G371), F356 (≠ V372), K357 (≠ A373)
- binding flavin mononucleotide: A61 (≠ G72), R62 (= R73), H171 (≠ P188), V250 (≠ Q271), E278 (≠ R299), R321 (≠ H338), W323 (= W340)
- binding 2-furoic acid: T64 (≠ D86), I66 (≠ V88), R68 (= R90), M263 (≠ D284), Y270 (= Y291), K357 (≠ A373)
- binding sulfite ion: K170 (≠ H187), K276 (≠ D297)
Sites not aligning to the query:
3rhaA The crystal structure of oxidoreductase from arthrobacter aurescens
47% identity, 14% coverage: 31:92/428 of query aligns to 4:71/459 of 3rhaA
- active site: S62 (≠ A83)
- binding dihydroflavine-adenine dinucleotide: V11 (= V38), G12 (= G39), G14 (= G41), P15 (≠ Y42), S16 (= S43), E35 (= E62), A36 (= A63), R37 (≠ E64), G42 (= G69), R43 (≠ A70), G57 (= G81), G58 (vs. gap), Q59 (vs. gap), W60 (vs. gap)
Sites not aligning to the query:
- active site: 197, 295
- binding dihydroflavine-adenine dinucleotide: 234, 235, 263, 384, 389, 394, 421, 422, 430, 431, 432
Query Sequence
>WP_053939623.1 NCBI__GCF_001294205.1:WP_053939623.1
MNARETAMVAHSWYEASVTRPPADAALQGALDVDVCIVGAGYSGLSAALELRQRGFSVAV
LEAEQVAWGASGRNGGQVLVGFANDDTVLRQLGPAAARQAWDISVAGVHLLHARVARYGI
DCDLVRGYLHVATSARKAEGLRHWTAQMRDTFGYDPISDIAPKDVRNWIDSPRYVGAAHD
ALSGHIHPLKYALGLADAARQAGVQLFAHSAVSRIEHGPLVTLHTAQGRVRSRFALIAAN
AYVGQLLGKTGARIMPVRSFIIATEPLPPEQAQALIRGRAAVCDTNFMIDYFRLSADGRV
LFGGRVTSGNAGPDALMPSLRARLAGVFPQLAHVAITHAWGGLVDVTMNRAPDFGRIAPN
VYYLQGFCGHGVALTGMAGQLVAEAIAGQAERFDLFARLQHHRYPGGPGLHSTWLRLGVA
YNQLKELV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory