SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_054034421.1 NCBI__GCF_001293685.1:WP_054034421.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
41% identity, 91% coverage: 12:258/270 of query aligns to 14:260/265 of P07821

query
sites
P07821

V

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K50 (= K48) mutation to Q: Lack of activity.
D172 (= D170) mutation to E: Lack of activity.
E173 (= E171) mutation to A: Lack of activity.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
34% identity, 84% coverage: 9:236/270 of query aligns to 2:223/371 of 3puyA

query
sites
3puyA

A

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binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F19), S37 (≠ N44), G38 (= G45), C39 (≠ S46), G40 (= G47), K41 (= K48), S42 (≠ T49), T43 (= T50), Q81 (= Q90), R128 (= R141), A132 (≠ Q145), S134 (= S147), G136 (= G149), Q137 (≠ E150), E158 (= E171), H191 (= H204)
binding magnesium ion: S42 (≠ T49), Q81 (= Q90)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
34% identity, 84% coverage: 9:236/270 of query aligns to 2:223/371 of 3puxA

query
sites
3puxA

A

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binding adenosine-5'-diphosphate: W12 (≠ F19), G38 (= G45), C39 (≠ S46), G40 (= G47), K41 (= K48), S42 (≠ T49), T43 (= T50), R128 (= R141), S134 (= S147), Q137 (≠ E150)
binding beryllium trifluoride ion: S37 (≠ N44), G38 (= G45), K41 (= K48), Q81 (= Q90), S134 (= S147), G136 (= G149), H191 (= H204)
binding magnesium ion: S42 (≠ T49), Q81 (= Q90)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
34% identity, 84% coverage: 9:236/270 of query aligns to 2:223/371 of 3puwA

query
sites
3puwA

A

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binding adenosine-5'-diphosphate: W12 (≠ F19), V17 (= V24), G38 (= G45), C39 (≠ S46), G40 (= G47), K41 (= K48), S42 (≠ T49), T43 (= T50), R128 (= R141), A132 (≠ Q145), S134 (= S147), Q137 (≠ E150)
binding tetrafluoroaluminate ion: S37 (≠ N44), G38 (= G45), K41 (= K48), Q81 (= Q90), S134 (= S147), G135 (= G148), G136 (= G149), E158 (= E171), H191 (= H204)
binding magnesium ion: S42 (≠ T49), Q81 (= Q90)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
34% identity, 84% coverage: 9:236/270 of query aligns to 2:223/371 of 3puvA

query
sites
3puvA

A

S

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F

Y

binding adenosine-5'-diphosphate: W12 (≠ F19), V17 (= V24), G38 (= G45), C39 (≠ S46), G40 (= G47), K41 (= K48), S42 (≠ T49), T43 (= T50), R128 (= R141), A132 (≠ Q145), S134 (= S147), Q137 (≠ E150)
binding magnesium ion: S42 (≠ T49), Q81 (= Q90)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
34% identity, 84% coverage: 9:236/270 of query aligns to 3:224/371 of P68187

query
sites
P68187

A

S

V

V

E

Q

V

L

R

Q

A

N

V

V

G

T

H

K

A

A

F

W

D

G

G

E

Q

V

A

V

V

V

L

S

A

K

D

D

L

I

S

N

F

L

Q

D

V

I

P

H

T

E

G

G

G

E

F

F

F

V

I

V

I

F

I

V

G

G

P

P

N

S

G

G

S

C

G

G

K

K

T

S

T

T

L

L

K

R

L

M

T

I

A

A

G

G

L

L

L

E

T

T

V

I

Q

T

A

S

G

G

Q

D

I

L

A

F

V

I

H

G

S

E

R

K

A

R

I

M

G

N

D

D

Y

T

T

P

A

P

R

A

E

E

L

-

A

-

R

R

R

G

M

V

A

G

Y

M

V

V

P

F

Q

Q

S

S

V

Y

P
x
A

V

L

T

Y

F

P

A

H

F

L

T

S

V

V

L

A

Q

E

V

N

V

M

M

S

M

F

G

G

R

-

A

-

P

-

H

-

L

L

G

K

M

L

L

A

G

G

F

A

E
x
K

G

K

E

E

-

V

-

I

-

N

Q

Q

D

R

L
x
V

T

N

Q

Q

A
x
V

W

A

E
|
E

A

V

M

L

Q

Q

L

L

A
|
A

G

-

V

-

A

-

H

H

L

L

A

L

D

D

R

R

R

K

L

P

D

K

Q

A

L

L

S

S

G

G

G
|
G

E

Q

R

R

Q

Q

R

R

V

V

F

A

I

I

A

G

R

R

A

T

I

L

C

V

Q

A

Q

E

P

P

E

S

I

V

M

F

L

L

D
|
D

E

E

P

P

T

L

A

S

A

N

L

L

D

D

L

A

A

A

H

L

Q

R

V

V

R

Q

V

M

M

R

D

I

L

E

M

I

A

S

R

R

L

L

K

H

T

K

E

R

E

L

G

G

M

R

T

T

V

M

V

I

M

Y

V

V

S

T

H

H

D

D

L

Q

N

V

L

E

A

A

A

M

M

T

Y

L

A

A

D

D

R

K

L

I

L

V

L

L

D

D

R

A

G

G

R

R

L

V

A

A

G

Q

I

V

G

G

P

K

P

P

A

L

E

E

V

L

L

Y

R

H

F

Y

A85 (≠ P93) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ E118) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ L123) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A126) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E128) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (= A133) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G149) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D170) mutation to N: Loss of maltose transport but retains ability to repress mal genes.

Sites not aligning to the query:

228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
34% identity, 84% coverage: 9:236/270 of query aligns to 2:223/374 of 2awnB

query
sites
2awnB

A

S

V

V

E

Q

V

L

R

Q

A

N

V

V

G

T

H

K

A

A

F
x
W

D

G

G

E

Q

V

A

V

V
|
V

L

S

A

K

D

D

L

I

S

N

F

L

Q

D

V

I

P

H

T

E

G

G

G

E

F

F

F

V

I

V

I

F

I

V

G

G

P

P

N
x
S

G
|
G

S

C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

K

R

L

M

T

I

A

A

G

G

L

L

L

E

T

T

V

I

Q

T

A

S

G

G

Q

D

I

L

A

F

V

I

H

G

S

E

R

K

A

R

I

M

G

N

D

D

Y

T

T

P

A

P

R

A

E

E

L

-

A

-

R

R

R

G

M

V

A

G

Y

M

V

V

P

F

Q

Q

S

S

V

Y

P

A

V

L

T

Y

F

P

A

H

F

L

T

S

V

V

L

A

Q

E

V

N

V

M

M

S

M

F

G

G

R

-

A

-

P

-

H

-

L

L

G

K

M

L

L

A

G

G

F

A

E

K

G

K

E

E

-

V

-

I

-

N

Q

Q

D

R

L

V

T

N

Q

Q

A

V

W

A

E

E

A

V

M

L

Q

Q

L

L

A

A

G

-

V

-

A

-

H

H

L

L

A

L

D

D

R

R

R

K

L

P

D

K

Q

A

L

L

S

S

G

G

E

Q

R

R

Q

Q

R

R

V

V

F

A

I

I

A

G

R

R

A

T

I

L

C

V

Q

A

Q

E

P

P

E

S

I

V

M

F

L

L

D

D

E

E

P

P

T

L

A

S

A

N

L

L

D

D

L

A

A

A

H

L

Q

R

V

V

R

Q

V

M

M

R

D

I

L

E

M

I

A

S

R

R

L

L

K

H

T

K

E

R

E

L

G

G

M

R

T

T

V

M

V

I

M

Y

V

V

S

T

H

H

D

D

L

Q

N

V

L

E

A

A

A

M

M

T

Y

L

A

A

D

D

R

K

L

I

L

V

L

L

D

D

R

A

G

G

R

R

L

V

A

A

G

Q

I

V

G

G

P

K

P

P

A

L

E

E

V

L

L

Y

R

H

F

Y

binding adenosine-5'-diphosphate: W12 (≠ F19), V17 (= V24), S37 (≠ N44), G38 (= G45), G40 (= G47), K41 (= K48), S42 (≠ T49), T43 (= T50)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
34% identity, 84% coverage: 10:236/270 of query aligns to 1:221/367 of 1q12A

query
sites
1q12A

V

V

E

Q

V

L

R

Q

A

N

V

V

G

T

H

K

A

A

F
x
W

D

G

G

E

Q

V

A

V

V

V

L

S

A

K

D

D

L

I

S

N

F

L

Q

D

V

I

P

H

T

E

G

G

G

E

F

F

F

V

I

V

I

F

I

V

G

G

P

P

N
x
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

K

R

L

M

T

I

A

A

G

G

L

L

L

E

T

T

V

I

Q

T

A

S

G

G

Q

D

I

L

A

F

V

I

H

G

S

E

R

K

A

R

I

M

G

N

D

D

Y

T

T

P

A

P

R

A

E

E

L

-

A

-

R

R

R

G

M

V

A

G

Y

M

V

V

P

F

Q

Q

S

S

V

Y

P

A

V

L

T

Y

F

P

A

H

F

L

T

S

V

V

L

A

Q

E

V

N

V

M

M

S

M

F

G

G

R

-

A

-

P

-

H

-

L

L

G

K

M

L

L

A

G

G

F

A

E

K

G

K

E

E

-

V

-

I

-

N

Q

Q

D

R

L

V

T

N

Q

Q

A

V

W

A

E

E

A

V

M

L

Q

Q

L

L

A

A

G

-

V

-

A

-

H

H

L

L

A

L

D

D

R
|
R

R

K

L

P

D

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

E
x
Q

R

R

Q

Q

R

R

V

V

F

A

I

I

A

G

R

R

A

T

I

L

C

V

Q

A

Q

E

P

P

E

S

I

V

M

F

L

L

D

D

E

E

P

P

T

L

A

S

A

N

L

L

D

D

L

A

A

A

H

L

Q

R

V

V

R

Q

V

M

M

R

D

I

L

E

M

I

A

S

R

R

L

L

K

H

T

K

E

R

E

L

G

G

M

R

T

T

V

M

V

I

M

Y

V

V

S

T

H

H

D

D

L

Q

N

V

L

E

A

A

A

M

M

T

Y

L

A

A

D

D

R

K

L

I

L

V

L

L

D

D

R

A

G

G

R

R

L

V

A

A

G

Q

I

V

G

G

P

K

P

P

A

L

E

E

V

L

L

Y

R

H

F

Y

binding adenosine-5'-triphosphate: W10 (≠ F19), S35 (≠ N44), G36 (= G45), C37 (≠ S46), G38 (= G47), K39 (= K48), S40 (≠ T49), T41 (= T50), R126 (= R141), A130 (≠ Q145), S132 (= S147), G134 (= G149), Q135 (≠ E150)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
34% identity, 84% coverage: 9:236/270 of query aligns to 3:224/369 of P19566

query
sites
P19566

A

S

V

V

E

Q

V

L

R

R

A

N

V

V

G

T

H

K

A

A

F

W

D

G

G

D

Q

V

A

V

V

V

L

S

A

K

D

D

L

I

S

N

F

L

Q

D

V

I

P

H

T

D

G

G

G

E

F

F

F

V

I

V

I

F

I

V

G

G

P

P

N

S

G

G

S

C

G

G

K

K

T

S

T

T

L

L

K

R

L

M

T

I

A

A

G

G

L

L

L

E

T

T

V

I

Q

T

A

S

G

G

Q

D

I

L

A

F

V

I

H

G

S

E

R

T

A

R

I

M

G

N

D

D

Y

I

T

P

A

P

R

A

E

E

L

-

A

-

R

R

R

G

M

V

A

G

Y

M

V

V

P

F

Q

Q

S

S

V

Y

P

A

V
x
L

T

Y

F

P

A

H

F

L

T

S

V

V

L

A

Q

E

V

N

V

M

M

S

M

F

G

G

R

-

A

-

P

-

H

-

L

L

G

K

M

L

L

A

G

G

F

A

E

K

G

K

E

E

-

V

-

M

-

N

Q

Q

D

R

L

V

T

N

Q

Q

A

V

W

A

E

E

A

V

M

L

Q

Q

L

L

A

A

G

-

V

-

A

-

H

H

L

L

A

L

D

E

R

R

R

K

L

P

D

K

Q

A

L

L

S

S

G

G

E

Q

R

R

Q

Q

R

R

V

V

F

A

I

I

A

G

R

R

A

T

I

L

C

V

Q

A

Q

E

P

P

E

R

I

V

M

F

L

L

D

D

E

E

P
|
P

T

L

A

S

A

N

L

L

D
|
D

L

A

A

A

H

L

Q

R

V

V

R

Q

V

M

M

R

D

I

L

E

M

I

A

S

R

R

L

L

K

H

T

K

E

R

E

L

G

G

M

R

T

T

V

M

V

I

M

Y

V

V

S

T

H

H

D

D

L

Q

N

V

L

E

A

A

A

M

M

T

Y

L

A

A

D

D

R

K

L

I

L

V

L

L

D

D

R

A

G

G

R

R

L

V

A

A

G

Q

I

V

G

G

P

K

P

P

A

L

E

E

V

L

L

Y

R

H

F

Y

L86 (≠ V94) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P172) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D177) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
33% identity, 85% coverage: 5:233/270 of query aligns to 11:235/378 of P69874

query
sites
P69874

P

P

Q

S

S

S

I

L

A

S

-

P

-

L

V

V

E

Q

V

L

R

A

A

G

V

I

G

R

H

K

A
x
C

F
|
F

D

D

G

G

Q

K

A

E

V

V

L

I

A

P

D

Q

L

L

S

D

F

L

Q

T

V

I

P

N

T

N

G

G

G

E

F
|
F

F

L

I

T

I

L

G

G

P

P

N

S

G

G

S
x
C

G

G

K

K

T

T

L

V

L
|
L

K

R

L

L

T

I

A

A

G

G

L

L

L

E

T

T

V

V

Q

D

A

S

G

G

Q

R

I

I

A

M

V
x
L

H

D

S

N

R

E

A

D

I

I

G

T

D

H

Y

V

T

P

A

A

R

E

E

N

L

-

A

-

R

R

R

Y

M

V

A

N

Y

T

V

V

P

F

Q

Q

S

S

V

Y

P

A

V

L

T

F

F

P

A

H

F

M

T

T

V

V

L

F

Q

E

V

N

V

V

M

A

M

F

G

G

R

-

A

-

P

-

H

-

L

L

G

R

M

M

L

Q

G

K

F

T

E

P

G

A

E

A

Q

E

D

I

L

T

T

P

Q

R

A

V

W

M

E

E

A

A

M

L

Q

R

L

M

A
x
V

G

Q

V

L

A

E

H

T

L

F

A

A

D

Q

R

R

R

K

L

P

D

H

Q

Q

L

L

S

S

G

G

E

Q

R

Q

Q

Q

R

R

V

V

F

A

I

I

A

A

R

R

A

A

I

V

C

V

Q

N

Q

K

P

P

E

R

I

L

M

L

L

L

D
|
D

E

E

P

S

T

L

A

S

A

A

L

L

D

D

L

Y

A

K

H

L

Q

R

V

K

R

Q

V

M

M

Q

D

N

L

E

M

L

A

K

R

A

L

L

K

Q

T

R

E

K

E

L

G

G

M

I

T

T

V

F

V

V

M

F

V

V

S

T

H

H

D

D

L

Q

N

E

L

E

A

A

A

L

M

T

Y

M

A

S

D

D

R

R

L

I

L

V

L

M

D

R

R

D

G

G

R

R

L

I

A

E

G

Q

I

D

G

G

P

T

P

P

A

R

E

E

V

I

C26 (≠ A18) mutation to A: Lower ATPase activity and transport efficiency.
F27 (= F19) mutation to L: Lower ATPase activity and transport efficiency.
F45 (= F37) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ S46) mutation to T: Loss of ATPase activity and transport.
L60 (= L52) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ V68) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ A133) mutation to M: Loss of ATPase activity and transport.
D172 (= D170) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
35% identity, 83% coverage: 10:234/270 of query aligns to 3:224/241 of 4u00A

query
sites
4u00A

V

I

E

R

V

I

R

R

A

N

V

L

G

H

H

K

A

W

F
|
F

D

G

G

P

Q

L

A

H

V
|
V

L

L

A

K

D

G

L

I

S

H

F

L

Q

E

V

V

P

A

T

P

G

G

G

E

F

K

F

L

I

V

I

I

G

G

P

P

N
x
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

I

K

R

L

T

T

I

A

N

G

R

L

L

L

E

T

D

V

F

Q

Q

A

E

G

G

Q

E

I

V

A

V

V

V

H

D

S

G

R

L

A

S

I

V

G

K

D

D

Y

D

T

R

A

A

-

L

R

R

E

E

L

I

A

R

R

R

R

E

M

V

A

G

Y

M

V

V

P

F

Q

Q

S

Q

V

F

P

N

V

L

T

F

F

P

A

H

F

M

T

T

V

V

L

L

Q

E

V

N

V

V

M

T

M

L

G

-

R

-

A

A

P

P

H

-

L

M

G

R

M

V

L

R

G

R

F

W

E

P

G

R

E

E

Q

K

D

A

L

E

T

K

Q

K

A

A

W

L

E

E

A

L

M

L

Q

E

L

R

A

V

G

G

V

I

A

L

H

D

L

Q

A

A

D

R

R

K

R

Y

L

P

D

A

Q

Q

L

L

S

S

G

G

E

Q

R

Q

Q

Q

R

R

V

V

F

A

I

I

A

A

R

R

A

A

I

L

C

A

Q

M

Q

E

P

P

E

K

I

I

M

M

L

L

L

F

D

D

E

E

P

P

T

T

A

S

A

A

L

L

D

D

L

P

A

E

H

M

Q

V

V

G

R

E

V

V

M

L

D

D

L

V

M

M

A

R

R

D

L

L

K

-

T

A

E

Q

E

G

G

G

M

M

T

T

V

M

V

V

M

V

V

V

S

T

H

H

D

E

L

M

N

G

L

F

A

A

A

R

M

E

Y

V

A

A

D

D

R

R

L

V

L

F

L

M

D

D

R

G

G

G

R

Q

L

I

A

V

G

E

I

E

G

G

P

R

P

P

A

E

E

E

V

I

L

F

binding adenosine-5'-diphosphate: F12 (= F19), V17 (= V24), S37 (≠ N44), G38 (= G45), S39 (= S46), G40 (= G47), K41 (= K48), S42 (≠ T49), T43 (= T50)

5x40A Structure of a cbio dimer bound with amppcp (see paper)
38% identity, 82% coverage: 14:234/270 of query aligns to 9:228/280 of 5x40A

query
sites
5x40A

A

A

V

L

G

T

H

Y

A

A

F
|
F

D

P

G

G

Q

G

-
x
V

A

K

V
x
A

L

L

A

D

D

D

L

L

S

S

F

L

Q

A

V

V

P

P

T

K

G

G

G

E

F

S

F

L

I

A

I

I

I

L

G

G

P

P

N
|
N

G
|
G

S

A

G
|
G

K
|
K

T
x
S

T
|
T

L

L

K

L

L

H

T

L

A

N

G

G

L

T

L

L

T

R

V

P

Q

Q

A

S

G

G

Q

R

I

V

A

L

V

L

H

G

S

G

R

T

A

A

I

T

G

G

D

-

Y

H

T

S

A

R

R

K

E

D

L

L

A

T

-

G

-

W

-

R

R

R

M

V

A

G

Y

L

V

V

P

L

Q
|
Q

S

D

V

A

P

D

-

D

V

Q

T

L

F

F

A

A

F

T

T

T

V

V

L

F

Q

E

V

D

V

V

M

S

M

F

G

G

R

P

A

L

P

-

H

N

L

L

G

G

M

L

L

S

G

E

F

A

E

E

G

A

E

R

Q

A

D

R

L

V

T

E

Q

E

A

A

W

L

E

A

A

A

M

L

Q

S

L

-

A

-

G

-

V

I

A

S

H

D

L

L

A

R

D

D

R

R

R

P

L

T

D
x
H

Q
x
M

L
|
L

S
|
S

G

G

G
|
G

E
x
Q

R

K

Q

R

R

R

V

V

F

A

I

I

A

A

R

G

A

A

I

V

C

A

Q

M

Q

R

P

P

E

E

I

V

M

L

L

L

D

D

E
x
Q

P

P

T

T

A

A

A

G

L

L

D

D

L

L

A

A

H

G

Q

T

V

E

R

Q

V

L

M

L

D

T

L

L

M

L

A

R

R

G

L

L

K

R

T

A

E

-

E

A

G

G

M

M

T

T

V

L

V

V

M

F

V

S

S

T

H
|
H

D

D

L

V

N

E

L

L

A

A

M

A

Y

L

A

A

D

D

R

R

L

V

L

A

L

L

L

F

D

R

R

T

G

G

R

R

L

V

A

L

G

A

I

E

G

G

P

A

A

E

E

A

V

V

L

L

binding phosphomethylphosphonic acid adenylate ester: F14 (= F19), V18 (vs. gap), A20 (≠ V24), N40 (= N44), G41 (= G45), G43 (= G47), K44 (= K48), S45 (≠ T49), T46 (= T50), Q88 (= Q90), H139 (≠ D144), M140 (≠ Q145), L141 (= L146), S142 (= S147), G144 (= G149), Q145 (≠ E150), Q166 (≠ E171), H198 (= H204)
binding magnesium ion: S45 (≠ T49), Q88 (= Q90)

Q5M243 Energy-coupling factor transporter ATP-binding protein EcfA1; ECF transporter A component EcfA1; EC 7.-.-.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
33% identity, 78% coverage: 24:234/270 of query aligns to 19:225/276 of Q5M243

query
sites
Q5M243

V

T

L

L

A

N

D

D

L

V

S

S

F

F

Q

H

V

V

P

K

T

H

G

G

G

E

F

W

F

L

I

S

I

I

I

V

G

G

P

H

N

N

G

G

S

S

G

G

K

K

T

S

T

T

L

T

L

A

K

R

L

L

T

I

A

G

G

G

L

L

T

V

V

A

Q

D

A

S

G

G

Q

Q

I

I

A

I

V

V

H

D

S

G

R

Q

A

E

I

L

G

T

D

E

Y

E

T

T

A

V

R

W

E

D

L

I

A

R

R

D

R

K

M

I

A

G

Y

M

V

V

P

F

Q

Q

S

N

V

P

P

D

V

N

T
x
Q

F

F

-

V

A

G

F

A

T

T

V

V

L

E

Q

D

V

D

V

V

M

A

M

F

G

G

R

-

A

-

P

-

H

-

L

L

G

E

M

N

L

K

G

G

F

L

E

P

G

Y

E

K

Q

E

D

M

L

V

T

S

Q

R

A

V

W

Q

E

E

A

A

M

L

Q

S

L

F

A

V

G

G

V

M

A

M

H

D

L

F

A

K

D

D

R

R

R

E

L

P

D

A

Q

R

L

L

S

S

G

G

E

Q

R

K

Q

Q

R

R

V

V

F

A

I

I

A

A

R

G

A

I

I

I

C

A

Q

M

Q

R

P

P

E

S

I

I

M

L

L

I

L

L

D

D

E
|
E

P

A

T

T

A

S

A

M

L

L

D

D

L

P

A

E

H

G

Q

R

V

Q

R

E

V

L

M

I

D

Q

L

Y

M

I

A

E

R

D

L

I

K

R

T

Q

E

Q

E

Y

G

G

M

M

T

T

V

V

V

L

M

S

V

I

S

T

H

H

D

D

L

L

N

D

L

E

A

V

A

A

M

M

Y

-

A

S

D

N

R

R

L

V

L

L

L

V

L

L

D

K

R

Q

G

G

R

K

L

V

A

E

G

S

I

I

G

S

P

S

P

P

A

R

E

E

V

L

L

F

Q90 (≠ T95) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-97 in EcfA2.
E163 (= E171) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-171 in EcfA2.

3d31A Modbc from methanosarcina acetivorans (see paper)
30% identity, 83% coverage: 10:234/270 of query aligns to 2:216/348 of 3d31A

query
sites
3d31A

V

I

E

E

V

I

R

E

A

S

V

L

G

S

H

R

A

K

F

W

D

K

G

N

Q

F

A

S

V

-

L

L

A

D

D

N

L

L

S

S

F

L

Q

K

V

V

P

E

T

S

G

G

G

E

F

Y

F

F

I

V

I

I

I

L

G

G

P

P

N

T

G

G

S

A

G

G

K

K

T

T

T

L

L

F

L

L

K

E

L

L

T

I

A

A

G

G

L

F

L

H

T

V

V

P

Q

D

A

S

G

G

Q

R

I

I

A

L

V

L

H

D

S

G

R

K

A

D

I

V

G

T

D

D

Y

L

T

S

A

P

R

E

E

K

L

-

A

-

R

H

R

D

M

I

A

A

Y

F

V

V

P

Y

Q

Q

S

N

V

Y

P

S

V

L

T

F

F

P

A

H

F

M

T

N

V

V

L

K

Q

K

V

N

V

L

M

E

M

F

G

G

R

-

A

-

P

-

H

-

L

-

G

-

M

-

L

M

G

R

F

M

E

K

G

K

E

I

Q

K

D

D

L

P

T

K

Q

R

A

V

W

L

E

D

A

T

M

A

Q

R

L

D

A

L

G

K

V

I

A

E

H

H

L

L

A

L

D

D

R

R

R

N

L

P

D

L

Q

T

L

L

S

S

G

G

E

E

R

Q

Q

Q

R

R

V

V

F

A

I

L

A

A

R

R

A

A

I

L

C

V

Q

T

Q

N

P

P

E

K

I

I

M

L

L

L

D

D

E

E

P

P

T

L

A

S

A

A

L

L

D

D

L

P

A

R

H

T

Q

Q

V

E

R

N

V

A

M

R

D

E

L

M

M

L

A

S

R

V

L

L

K

H

T

K

E

K

E

N

G

K

M

L

T

T

V

V

V

L

M

H

V

I

S

T

H

H

D

D

L

Q

N

T

L

E

A

A

A

R

M

I

Y

M

A

A

D

D

R

R

L

I

L

A

L

V

D

M

R

D

G

G

R

K

L

L

A

I

G

Q

I

V

G

G

P

K

P

P

A

E

E

E

V

I

L

F

Sites not aligning to the query:

binding tungstate(vi)ion: 286, 320, 323, 340, 341, 342

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
33% identity, 74% coverage: 25:224/270 of query aligns to 21:223/232 of 1f3oA

query
sites
1f3oA

L

L

A

K

D

N

L

V

S

N

F

L

Q

N

V

I

P

K

T

E

G

G

G

E

F

F

F

V

I

S

I

I

I

M

G

G

P

P

N

S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

M

L

L

K

N

L

I

T

I

A

G

G

C

L

L

L

D

T

K

V

P

Q

T

A

E

G

G

Q

E

I

V

A

Y

V

I

H

D

S

N

R

I

A

K

I

T

G

N

D

D

Y

L

T

D

A

D

R

D

E

E

L

L

A

T

R

K

-

I

-

R

-

D

R

K

M

I

A

G

Y

F

V

V

P

F

Q

Q

S

Q

V

F

P

N

V

L

T

I

F

P

A

L

F

L

T

T

V

A

L

L

Q

E

V

N

V

V

M

E

M

L

G

P

R

L

A

I

-

F

P

K

H

Y

L

R

G

G

M

A

L

M

G

S

F

-

E

-

G

G

E

E

Q

E

D

R

L

R

T

K

Q

R

A

A

W

L

E

E

A

C

M

L

Q

K

L

M

A

A

G

E

V

L

A

E

H

E

-

R

L

F

A

A

D

N

R

H

R

K

L

P

D

N

Q

Q

L

L

S

S

G

G

E

Q

R

Q

Q

Q

R

R

V

V

F

A

I

I

A

A

R

R

A

A

I

L

C

A

Q

N

P

P

E

P

I

I

M

I

L

L

L

A

D

D

E

E

P

P

T

T

A

G

A

A

L

L

D

D

L

S

A

K

H

T

Q

G

V

E

R

K

V

I

M

M

D

Q

L

L

M

L

A

K

R

K

L

L

K

N

T

E

E

E

E

D

G

G

M

K

T

T

V

V

M

V

V

V

S

T

H

H

D

D

L

I

N

N

L

V

A

A

A

R

M

-

Y

F

A

G

D

E

R

R

L

I

L

Y

L

L

D

K

R

D

G

G

R

E

L

V

binding adenosine-5'-diphosphate: G41 (= G45), G43 (= G47), K44 (= K48), S45 (≠ T49), T46 (= T50)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 11

1g291 Malk (see paper)
32% identity, 81% coverage: 15:233/270 of query aligns to 9:227/372 of 1g291

query
sites
1g291

V

V

G

W

H

K

A

V

F

F

D

G

G

E

Q

V

A

T

V

A

L

V

A

R

D

E

L

M

S

S

F

L

Q

E

V

V

P

K

T

D

G

G

G

E

F

F

F

M

I

I

I

L

G

G

P

P

N
x
S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

L

T

L

L

K

R

L

M

T

I

A

A

G

G

L

L

L

E

T

E

V

P

Q

S

A

R

G

G

Q

Q

I

I

A

Y

V

I

H

G

S

D

R

K

A

L

I

V

G

A

D
|
D

Y

P

T
x
E

A
x
K

R

G

E

I

L

F

A

V

-

P

-
x
K

-
x
D

R

R

R

D

M

I

A

A

Y

M

V

V

P

F

Q

Q

S

S

V

Y

P

A

V

L

T

Y

F

P

A

H

F

M

T

T

V

V

L

Y

Q

D

V

N

V

I

M

-

G

-

R

-

A

A

P

F

H

P

L

L

G

K

M

L

L

R

G

K

F

V

E

P

G

R

E

Q

Q

E

D

I

L

D

T

Q

Q

R

A

V

W

R

E

E

A

V

M

A

Q

E

L

L

A

L

G

G

V

L

A

T

H

E

L

L

A

L

D

N

R

R

R

K

L

P

D

R

Q

E

L

L

S

S

G

G

E

Q

R

R

Q

Q

R

R

V

V

F

A

I

L

A

G

R

R

A

A

I

I

C

V

Q

R

Q

K

P

P

E

Q

I

V

M

F

L

L

L

M

D

D

E

E

P

P

T

L

A

S

A

N

L

L

D

D

L

A

A

K

H

L

Q

R

V

V

R

R

V

M

M

R

D

A

L

E

M

L

A

K

R

K

L

L

K

Q

T

R

E

Q

E

L

G

G

M

V

T

T

V

T

V

I

M

Y

V

V

S

T

H

H

D

D

L

Q

N

V

L

E

A

A

A

M

M

T

Y

M

A

G

D

D

R

R

L

I

L

A

L

V

L

M

D

N

R

R

G

G

R

V

L

L

A

Q

G

Q

I

V

G

G

P

S

P

P

A

D

E

E

V

V

binding magnesium ion: D69 (= D75), E71 (≠ T77), K72 (≠ A78), K79 (vs. gap), D80 (vs. gap)
binding pyrophosphate 2-: S38 (≠ N44), G39 (= G45), C40 (≠ S46), G41 (= G47), K42 (= K48), T43 (= T49), T44 (= T50)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 359

8bmpA Cryo-em structure of the folate-specific ecf transporter complex in msp2n2 lipid nanodiscs bound to atp and adp (see paper)
35% identity, 81% coverage: 25:242/270 of query aligns to 20:237/278 of 8bmpA

query
sites
8bmpA

L

L

A

S

D

D

L

L

S

S

F

F

Q

A

V

I

P

E

T

R

G

G

G

S

F

W

F

T

I

A

I

L

I

I

G

G

P

H

N
|
N

G

G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

V

L

S

K

K

L

L

T

I

A

N

G

G

L

L

T

A

-

P

-

D

V

L

Q

D

A

K

G

S

Q

S

I

I

A

T

V

V

H

D

S

G

R

V

A

K

I

L

G

G

D

A

Y

D

T

T

A

V

R

W

E

E

L

V

A

R

R

E

R

K

M

V

A

G

Y

I

V

V

P

F

Q

Q

S

N

V

P

P

D

V

N

T

Q

F

F

-

V

A

G

F

A

T

T

V

V

L

S

Q

D

V

D

V

V

M

A

M

F

G

G

-

L

-

E

-

N

R

R

A

A

-

V

P

P

H

R

L

P

G

E

M

M

L

L

G

K

F

I

E

V

G

A

E

Q

Q

-

D

-

L

-

T

-

Q

-

A

-

W

-

E

-

A

A

M

V

Q

A

L

D

A

V

G

G

V

M

A

A

H

D

L

Y

A

A

D

D

R

S

R

E

L

P

D

S

Q

N

L

L

S

S

G

G

E

Q

R

K

Q

Q

R

R

V

V

F

A

I

I

A

A

R

G

A

I

I

L

C

A

Q

V

Q

K

P

P

E

Q

I

V

M

I

L

I

L

L

D

D

E

E

P

S

T

T

A

S

A

M

L

L

D

D

L

P

A

E

H

G

Q

K

V

E

R

Q

V

I

M

L

D

D

L

L

M

V

A

R

R

K

L

I

K

K

T

E

E

D

E

N

G

N

M

L

T

T

V

V

V

I

M

S

V

I

S

T

H

H

D

D

L

L

N

E

L

E

A

A

M

-

Y

G

A

A

D

D

R

Q

L

V

L

L

L

V

L

L

D

D

R

D

G

G

R

Q

L

L

A

L

G

D

I

Q

G

G

P

K

P

P

A

E

E

E

V

I

L

F

-

P

R

K

F

V

D

E

L

M

L

L

E

K

R

R

V

I

binding adenosine-5'-triphosphate: N39 (= N44), G42 (= G47), K43 (= K48), S44 (≠ T49), T45 (= T50)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 13

5d3mA Folate ecf transporter: amppnp bound state (see paper)
35% identity, 81% coverage: 25:242/270 of query aligns to 23:240/280 of 5d3mA

query
sites
5d3mA

L

L

A

S

D

D

L

L

S

S

F

F

Q

A

V

I

P

E

T

R

G

G

G

S

F

W

F

T

I

A

I

L

I

I

G

G

P

H

N
|
N

G
|
G

S

S

G

G

K
|
K

T
x
S

T

T

L

V

L

S

K

K

L

L

T

I

A

N

G

G

L

L

T

A

-

P

-

D

V

L

Q

D

A

K

G

S

Q

S

I

I

A

T

V

V

H

D

S

G

R

V

A

K

I

L

G

G

D

A

Y

D

T

T

A

V

R

W

E

E

L

V

A

R

R

E

R

K

M

V

A

G

Y

I

V

V

P

F

Q

Q

S

N

V

P

P

D

V

N

T

Q

F

F

-

V

A

G

F

A

T

T

V

V

L

S

Q

D

V

D

V

V

M

A

M

F

G

G

-

L

-

E

-

N

R

R

A

A

-

V

P

P

H

R

L

P

G

E

M

M

L

L

G

K

F

I

E

V

G

A

E

Q

Q

-

D

-

L

-

T

-

Q

-

A

-

W

-

E

-

A

A

M

V

Q

A

L

D

A

V

G

G

V

M

A

A

H

D

L

Y

A

A

D

D

R

S

R

E

L

P

D

S

Q

N

L

L

S

S

G

G

E

Q

R

K

Q

Q

R

R

V

V

F

A

I

I

A

A

R

G

A

I

I

L

C

A

Q

V

Q

K

P

P

E

Q

I

V

M

I

L

I

L

L

D

D

E

E

P

S

T

T

A

S

A

M

L

L

D

D

L

P

A

E

H

G

Q

K

V

E

R

Q

V

I

M

L

D

D

L

L

M

V

A

R

R

K

L

I

K

K

T

E

E

D

E

N

G

N

M

L

T

T

V

V

V

I

M

S

V

I

S

T

H

H

D

D

L

L

N

E

L

E

A

A

M

-

Y

G

A

A

D

D

R

Q

L

V

L

L

L

V

L

L

D

D

R

D

G

G

R

Q

L

L

A

L

G

D

I

Q

G

G

P

K

P

P

A

E

E

E

V

I

L

F

-

P

R

K

F

V

D

E

L

M

L

L

E

K

R

R

V

I

binding phosphoaminophosphonic acid-adenylate ester: N42 (= N44), G43 (= G45), K46 (= K48), S47 (≠ T49)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
30% identity, 83% coverage: 10:233/270 of query aligns to 7:230/375 of 2d62A

query
sites
2d62A

V

V

E

K

V

L

R

I

A

N

V

I

G

W

H

K

A

R

F

F

D

G

G

D

Q

V

A

T

V

A

L

V

A

K

D

D

L

L

S

S

F

L

Q

E

V

I

P

K

T

D

G

G

G

E

F

F

F

L

I

V

I

L

G

G

P

P

N

S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

L

T

L

L

K

R

L

M

T

I

A

A

G

G

L

L

L

E

T

E

V

P

Q

T

A

R

G

G

Q

Q

I

I

A

Y

V

I

H

E

S

D

R

N

A

L

I

V

G

A

D

D

Y

P

T

E

A

K

R

G

E

V

L

F

A

V

-

P

-

K

-

E

R

R

R

D

M

V

A

A

Y

M

V

V

P

F

Q

Q

S

S

V

Y

P

A

V

L

T

Y

F

P

A

H

F

M

T

T

V

V

L

Y

Q

D

V

N

V

I

M

-

G

-

R

-

A

A

P

F

H

P

L

L

G

K

M

L

L

R

G

K

F

V

E

P

G

K

E

Q

Q

E

D

I

L

D

T

K

Q

R

A

V

W

R

E

E

A

V

M

A

Q

E

L

M

A

L

G

G

V

L

A

T

H

E

L

L

A

L

D

N

R

R

R

K

L

P

D

R

Q

E

L

L

S

S

G

G

E

Q

R

R

Q

Q

R

R

V

V

F

A

I

L

A

G

R

R

A

A

I

I

C

I

Q

R

P

P

E

K

I

V

M

F

L

L

L

M

D

D

E

E

P

P

T

L

A

S

A

N

L

L

D

D

L

A

A

K

H

L

Q

R

V

V

R

K

V

M

M

R

D

A

L

E

M

L

A

K

R

K

L

L

K

Q

T

R

E

Q

E

L

G

G

M

V

T

T

V

T

V

I

M

Y

V

V

S

T

H

H

D

D

L

Q

N

V

L

E

A

A

A

M

M

T

Y

M

A

G

D

D

R

R

L

I

L

A

L

V

L

M

D

N

R

K

G

G

R

E

L

L

A

Q

G

Q

I

V

G

G

P

T

P

P

A

D

E

E

V

V

binding pyrophosphate 2-: G42 (= G45), C43 (≠ S46), G44 (= G47), K45 (= K48), T46 (= T49), T47 (= T50)

8bmsA Cryo-em structure of the mutant solitary ecf module 2eq in msp2n2 lipid nanodiscs in the atpase closed and atp-bound conformation (see paper)
34% identity, 81% coverage: 25:242/270 of query aligns to 20:237/278 of 8bmsA

query
sites
8bmsA

L

L

A

S

D

D

L

L

S

S

F

F

Q

A

V

I

P

E

T

R

G

G

G

S

F

W

F

T

I

A

I

L

I

I

G

G

P

H

N
|
N

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

T
|
T

L

V

L

S

K

K

L

L

T

I

A

N

G

G

L

L

T

A

-

P

-

D

V

L

Q

D

A

K

G

S

Q

S

I

I

A

T

V

V

H

D

S

G

R

V

A

K

I

L

G

G

D

A

Y

D

T

T

A

V

R

W

E

E

L

V

A

R

R

E

R

K

M

V

A

G

Y

I

V

V

P

F