SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_054256957.1 NCBI__GCF_001298675.1:WP_054256957.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2pg0A Crystal structure of acyl-coa dehydrogenase from geobacillus kaustophilus
46% identity, 96% coverage: 12:389/392 of query aligns to 6:378/380 of 2pg0A

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active site: M124 (= M132), T125 (= T133), E243 (= E254), A364 (≠ G375), R376 (= R387)
binding flavin-adenine dinucleotide: I122 (= I130), M124 (= M132), T125 (= T133), G130 (= G138), S131 (= S139), F155 (= F167), I156 (= I168), T157 (≠ S169), R269 (= R280), F272 (= F283), F279 (= F290), Q337 (= Q348), L338 (= L349), G340 (= G351), G341 (= G352), V359 (= V370), I362 (= I373), Y363 (= Y374), T366 (= T377), E368 (= E379), M369 (≠ I380)

P51174 Long-chain specific acyl-CoA dehydrogenase, mitochondrial; LCAD; EC 1.3.8.8 from Mus musculus (Mouse) (see paper)
42% identity, 96% coverage: 13:390/392 of query aligns to 55:427/430 of P51174

query
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P51174

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K318 (≠ Q281) modified: N6-acetyllysine; mutation to R: Reduces activity by 37%; reduces activity by 80% when associated with R-322.
K322 (≠ Q285) modified: N6-acetyllysine; alternate; mutation to R: Reduces activity by 23%; reduces activity by 80% when associated with R-318.

Sites not aligning to the query:

42 modified: N6-acetyllysine; K→R: Reduces activity by 90% when associated with R-318 and R-322.

8w0uA Human lcad complexed with acetoacetyl coenzyme a (see paper)
41% identity, 96% coverage: 14:390/392 of query aligns to 24:395/398 of 8w0uA

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binding acetoacetyl-coenzyme a: M140 (= M132), S147 (= S139), Q150 (≠ K142), S193 (vs. gap), H196 (≠ K190), Y250 (≠ V245), E259 (= E254), R260 (= R255), Y379 (= Y374), G380 (= G375), G381 (= G376), I385 (= I380), L389 (= L384), R392 (= R387)
binding flavin-adenine dinucleotide: I138 (= I130), M140 (= M132), T141 (= T133), G146 (= G138), S147 (= S139), F171 (= F167), S173 (= S169), R285 (= R280), F288 (= F283), L295 (≠ F290), Q353 (= Q348), L354 (= L349), G357 (= G352), V375 (= V370), Y379 (= Y374), T382 (= T377), E384 (= E379)

8w0tA Human lcad (see paper)
41% identity, 96% coverage: 14:390/392 of query aligns to 24:395/398 of 8w0tA

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D

N

V

I

P

A

E

E

P

H

Y

L

G

G

G

G

A

I

G

G

A

G

S

D

V

L

H

-

F

Y

S

S

F

A

A

A

V

I

V

V

E

-

V

-

L

W

S

E

R

E

R

Q

G

A

Y

Y

G

S

G

N

F

C

V

S

G

G

-

P

G

G

L

F

Q

S

V

I

H

H

N

S

D

G

I

I

I

V

P

M

P

S

Y

Y

L

I

L

T

H

N

C

H

G

G

T

S

E

E

A

E

Q

Q

R

I

Q

K

Y

H

W

F

L

I

P

P

R

Q

M

M

V

T

S

A

G

G

E

K

A

C

V

I

A

G

A

A

I
|
I

G

A

M
|
M

T
|
T

E

E

P

P

G

G

A

A

G
|
G

S
|
S

D

D

L

L

K

Q

A

G

I

I

R

K

T

T

T

N

A

A

R

K

R

K

I

-

S

-

D

-

S

-

D

D

G

G

D

S

G

D

Y

W

V

I

I

L

N

N

G

G

S

S

K

K

I

V

F
|
F

I
|
I

S
|
S

N

N

G

G

Q

S

H

L

C

S

D

D

L

V

L

V

V

I

L

V

A

V

A

A

K

V

T

T

D

N

P

H

A

E

A

A

G

P

-

S

-

P

A

A

K

H

G

G

V

I

S

S

L

L

F

F

L

L

V

V

D

E

T

N

K

G

S

M

P

K

G

G

F

F

T

I

R

K

G

G

Q

R

N

K

L

L

E

H

K

K

I

M

G

G

Q

L

H

K

A

A

G

Q

D

D

T

T

S

A

E

E

L

L

F

F

F

F

N

E

D

D

L

I

R

R

V

L

P

P

Q

A

D

S

A

A

L

L

L

L

G

G

G

E

V

-

E

E

G

N

Q

K

G

G

F

F

V

Y

Q

Y

M

I

M

M

R

K

E

E

L

L

P

P

R

Q

E

E

R

R

L

L

I

L

I

I

G

A

V

D

Q

V

A

A

V

I

Y

S

G

A

A

S

K

E

G

F

A

M

L

F

D

E

A

E

T

T

V

R

K

N

Y

Y

V

V

Q

K

E

Q

R
|
R

Q

K

A

A

F
|
F

G

G

Q

K

A

T

I

V

G

A

Q

H

F
x
L

Q

Q

N

T

T

V

R

Q

F

H

T

K

L

L

A

A

Q

E

C

L

A

K

S

T

D

H

I

I

A

C

A

V

A

T

K

R

A

A

F

F

L

V

N

D

A

N

S

C

V

L

A

Q

A

L

Y

H

E

E

R

A

G

K

E

R

L

L

T

D

P

S

E

A

A

T

V

A

S

C

A

M

L

A

K

K

L

Y

H

W

T

A

T

S

E

E

V

L

F

Q

G

N

R

S

V

V

A

A

D

Y

A

D

C

C

L

V

Q
|
Q

L
|
L

F

H

G
|
G

G
|
G

Y

W

G

G

Y

Y

M

M

A

W

E

E

Y

Y

P

P

I

I

S

A

R

K

F

A

W

Y

T

V

D

D

A

A

R

R

V
|
V

L

Q

R

P

I

I

Y

Y

G

G

G

G

T
|
T

S

N

E
|
E

I
|
I

M

M

K

K

E

E

L

L

V

I

A

A

R

R

S

E

L

I

L

V

binding flavin-adenine dinucleotide: I138 (= I130), M140 (= M132), T141 (= T133), G146 (= G138), S147 (= S139), F171 (= F167), I172 (= I168), S173 (= S169), R285 (= R280), F288 (= F283), L295 (≠ F290), Q353 (= Q348), L354 (= L349), G356 (= G351), G357 (= G352), V375 (= V370), T382 (= T377), E384 (= E379), I385 (= I380)

P28330 Long-chain specific acyl-CoA dehydrogenase, mitochondrial; LCAD; EC 1.3.8.8 from Homo sapiens (Human) (see 2 papers)
41% identity, 96% coverage: 14:390/392 of query aligns to 56:427/430 of P28330

query
sites
P28330

E

E

L

H

Q

D

V

I

F

F

T

R

A

K

S

S

L

V

E

R

R

K

F

F

C

F

D

Q

T

E

E

E

I

V

E

I

P

P

H

H

Y

H

R

S

D

E

W

W

E

E

K

K

A

A

G

G

L

E

V

V

S

S

R

R

E

E

L

V

F

W

R

E

K

K

M

A

G

G

E

K

N

Q

G

G

Y

L

L

L

C

G

A

V

D

N

V

I

P

A

E

E

P

H

Y

L

G

G

G

G

A

I

G

G

A

G

S

D

V

L

H

-

F

Y

S

S

F

A

A

A

V

I

V

V

E

-

V

-

L

W

S

E

R

E

R

Q

G

A

Y

Y

G

S

G

N

F

C

V

S

G

G

-

P

G

G

L

F

Q

S

V

I

H

H

N

S

D

G

I

I

I

V

P

M

P

S

Y

Y

L

I

L

T

H

N

C

H

G

G

T

S

E

E

A

E

Q

Q

R

I

Q

K

Y

H

W

F

L

I

P

P

R

Q

M

M

V

T

S

A

G

G

E

K

A

C

V

I

A

G

A

A

I

I

G

A

M

M

T

T

E

E

P

P

G

G

A

A

G

G

S

S

D

D

L

L

K

Q

A

G

I

I

R

K

T

T

T

N

A

A

R

K

R

K

I

-

S

-

D

-

S

-

D

D

G

G

D

S

G

D

Y

W

V

I

I

L

N

N

G

G

S

S

K

K

I

V

F

F

I

I

S

S

N

N

G

G

Q

S

H

L

C

S

D

D

L

V

L

V

V

I

L

V

A

V

A

A

K

V

T

T

D

N

P

H

A

E

A

A

G

P

-

S

-

P

A

A

K

H

G

G

V

I

S

S

L

L

F

F

L

L

V

V

D

E

T

N

K

G

S

M

P

K

G

G

F

F

T

I

R

K

G

G

Q

R

N

K

L

L

E

H

K

K

I

M

G

G

Q

L

H

K

A

A

G

Q

D

D

T

T

S

A

E

E

L

L

F

F

F

F

N

E

D

D

L

I

R

R

V

L

P

P

Q

A

D

S

A

A

L

L

L

L

G

G

G

E

V

-

E

E

G

N

Q

K

G

G

F

F

V

Y

Q

Y

M

I

M

M

R

K

E

E

L

L

P

P

R

Q

E
|
E

R

R

L

L

I

L

I

I

G

A

V

D

Q

V

A

A

V

I

Y

S

G

A

A
x
S

K

E

G

F

A

M

L

F

D

E

A

E

T

T

V

R

K

N

Y

Y

V

V

Q

K

E

Q

R

R

Q

K

A

A

F

F

G

G

Q

K

A

T

I

V

G

A

Q

H

F

L

Q

Q

N

T

T

V

R

Q

F

H

T
x
K

L

L

A

A

Q

E

C

L

A

K

S

T

D

H

I

I

A

C

A

V

A

T

K

R

A

A

F

F

L

V

N

D

A

N

S

C

V

L

A

Q

A

L

Y

H

E

E

R

A

G

K

E

R

L

L

T

D

P

S

E

A

A

T

V

A

S

C

A

M

L

A

K

K

L

Y

H

W

T

A

T

S

E

E

V

L

F

Q

G

N

R

S

V

V

A

A

D

Y

A

D

C

C

L

V

Q

Q

L

L

F

H

G

G

G

G

Y

W

G

G

Y

Y

M

M

A

W

E

E

Y

Y

P

P

I

I

S

A

R

K

F

A

W

Y

T

V

D

D

A

A

R

R

V

V

L

Q

R

P

I

I

Y

Y

G

G

G

G

T

T

S

N

E

E

I

I

M

M

K

K

E

E

L

L

V

I

A

A

R

R

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E

L

I

L

V

E291 (= E254) active site, Proton acceptor; mutation to Q: Loss of long-chain-acyl-CoA dehydrogenase activity. No effect on protein abundance. No effect on solubility. No effect on substrate binding.
S303 (≠ A266) to T: in dbSNP:rs1801204
K333 (≠ T296) to Q: in dbSNP:rs2286963

4iv6B X-ray crystal structure of an isovaleryl-coa dehydrogenase from mycobacterium smegmatis (see paper)
38% identity, 97% coverage: 13:392/392 of query aligns to 4:378/383 of 4iv6B

query
sites
4iv6B

A

A

E

E

L

E

Q

E

V

T

F

I

T

V

A

K

S

T

L

V

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H

R

D

F

F

C

V

D

E

T

K

E

Q

I

V

E

K

P

P

H

V

Y

V

R

R

D

E

W

L

E

E

K

H

A

A

G

N

L

T

V

Y

S

P

R

E

E

E

L

L

F

I

R

E

K

T

M

M

G

K

E

E

N

I

G

G

Y

I

L

F

C

G

A

L

D

A

V

I

P

P

E

E

P

P

Y

Y

G

G

G

F

A

G

G

A

A

V

S

S

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M

H

P

F

C

S

Y

F

V

A

Q

V

V

V

A

E

E

V

E

L

L

S

A

R

R

R

-

G

G

Y

W

G

M

G

S

F

L

V

A

G

G

G

A

L

M

Q

G

V

G

H

H

N

T

D

-

I

V

I

V

P

S

P

K

Y

L

L

L

L

L

H

L

C

F

G

G

T

T

E

E

A

E

Q

Q

R

K

Q

Q

Y

K

W

Y

L

L

P

P

R

R

M

M

V

A

S

T

G

G

E

E

A

L

V

R

A

A

A

T

I

M

G

A

M
x
L

T
|
T

E

E

P

P

G

G

A
x
G

G
|
G

S
|
S

D

D

L

L

K

Q

A

A

I

M

R

R

T

T

T

V

A

A

R

R

R

R

I

-

S

-

D

-

S

-

D

D

G

G

D

D

G

D

Y

Y

V

V

I

I

N

N

G

G

S

S

K

K

I

T

F
x
W

I
|
I

S
|
S

N

N

G

A

Q

R

H

R

C

S

D

D

L

L

L

V

V

A

L

L

A

M

A

C

K

K

T

T

D

D

P

P

A

D

A

A

-

Q

-

P

G

A

A

H

K

K

G

G

V

V

S

S

L

I

F

L

L

L

V

V

D

E

T

-

K

K

S

V

P

P

G

G

F

F

T

D

R

V

G

S

Q

R

N

D

L

L

E

P

K

K

I

L

G

G

Q

Y

H

K

A

G

G

V

D

E

T

S

S

C

E

E

L

L

F

N

F

F

N

T

D

D

L

A

R

R

V

V

P

P

Q

V

D

S

A

S

L

L

L

L

G

G

G

D

V

D

E

E

G

G

Q

R

G

G

F

F

V

A

Q

Q

M

M

M

M

R

K

E

G

L

L

P

E

R

V

E
x
G

R

R

L

L

I

Q

I

V

G

A

V

A

Q

R

A

A

V

T

Y

G

G

V

A

A

K

R

G

A

A

A

L

F

D

E

A

D

T

A

V

L

K

R

Y

Y

V

S

Q

Q

E

E

R
|
R

Q

E

A
x
S

F
|
F

G

G

Q

K

A

P

I
|
I

G

W

Q

Q

F
x
H

Q

Q

N

S

T
x
V

R

G

F

N

T

M

L

L

A

A

Q

D

C

M

A

G

S

T

D

K

I

L

A

Y

A

A

A

A

K

R

A

S

F

L

L

L

N

L

A

S

S

A

V

A

A

E

A

K

Y

F

E

D

R

A

G

G

E

Q

L

R

T

C

P

D

E

M

A

E

V

A

S

G

A

M

L

A

K

K

L

L

H

F

T

A

T

S

E

E

V

T

F

A

G

M

R

Q

V

I

A

A

D

L

A

D

C

A

L

V

Q
x
R

L
x
V

F

H

G

G

G
|
G

Y

Y

G

G

Y

Y

M

S

A

T

E

E

Y

Y

P

D

I

V

S

E

R

R

F

Y

W

F

T

R

D

D

A

A

R

P

V
x
L

L

M

R

I

I

V

Y
x
G

G
x
E

G

G

T
|
T

S

N

E
|
E

I
|
I

M

Q

K

R

E

N

L

V

V

I

A

A

R
x
K

S

Q

L

L

L

V

G

A

R

R

active site: L121 (≠ M132), T122 (= T133), G240 (≠ E254), E361 (≠ G375), K373 (≠ R387)
binding dihydroflavine-adenine dinucleotide: L121 (≠ M132), T122 (= T133), G126 (≠ A137), G127 (= G138), S128 (= S139), W152 (≠ F167), I153 (= I168), S154 (= S169), R266 (= R280), S268 (≠ A282), F269 (= F283), I273 (= I287), H276 (≠ F290), V279 (≠ T293), R334 (≠ Q348), V335 (≠ L349), G338 (= G352), L356 (≠ V370), G360 (≠ Y374), T363 (= T377), E365 (= E379), I366 (= I380)

4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
38% identity, 94% coverage: 24:390/392 of query aligns to 18:378/380 of 4l1fA

query
sites
4l1fA

R

E

F

F

C

A

D

E

T

K

E

F

I

L

E

A

P

P

H

T

Y

V

R

E

D

E

W

R

E

D

K

K

A

A

G

H

L

I

V

W

S

D

R

R

E

K

L

L

F

I

R

D

K

K

M

M

G

G

E

E

N

A

G

G

Y

F

L

C

C

G

A

I

D

C

V

F

P

P

E

E

P

E

Y

Y

G

G

G

G

A

M

G

G

A

L

S

D

V

V

H

L

F

S

S

Y

F

I

A

L

V

A

V

V

E

E

V

E

L

L

S

S

R

K

R

V

G

D

Y

D

G

G

-

T

G

G

F

I

V

T

G

L

G

S

L

A

Q

N

V

V

H

S

N

L

D

C

I

A

I

T

P

P

P

I

Y

Y

L

M

L

F

H

-

C

-

G

G

T

T

E

E

A

E

Q

Q

R

K

Q

Q

Y

K

W

Y

L

L

P

A

R

P

M

I

V

A

S

E

G

G

E

T

A

H

V

V

A

G

A

A

I
x
F

G

G

M
x
L

T
|
T

E

E

P

P

G

S

A

A

G
|
G

S
x
T

D

D

L

A

K

S

A

A

I

Q

R

Q

T

T

T

T

A

A

R

V

R

L

I

-

S

-

D

-

S

-

D

K

G

G

D

D

G

K

Y

Y

V

I

I

L

N

N

G

G

S

S

K

K

I

I

F
|
F

I
|
I

S
x
T

N

N

G

G

Q

K

H

E

C

A

D

D

L

T

L

Y

V

V

L

V

A

F

A

A

K

M

T

T

D

D

P

K

A

S

A

Q

G

G

A

V

K
x
H

G

G

V

I

S

S

L

A

F

F

L

I

V

L

D

E

T

K

K

G

S

M

P

P

G

G

F

F

T

R

R

F

G

G

Q

K

N

I

L

E

E

D

K

K

I

M

G

G

Q

G

H

H

A

T

G

S

D

I

T

T

S

A

E

E

L

L

F

I

F

F

N

E

D

D

L

C

R

E

V

V

P

P

Q

K

D

E

A

N

L

L

L

L

G

G

G

K

V

-

E

E

G

G

Q

E

G

G

F
|
F

V

K

Q

I

M

A

M
|
M

R
x
E

E

T

L
|
L

P
x
D

R

G

E
x
G

R
|
R

L

I

I

G

I

V

G

A

V

A

Q

Q

A

A

V

L

Y

G

G

I

A

A

K

E

G

G

A

A

L

L

D

A

A

A

T

A

V

V

K

K

Y

Y

V

S

Q

K

E

E

R
|
R

Q

E

A
x
Q

F
|
F

G

G

Q

R

A

S

I
|
I

G

S

Q

K

F
|
F

Q

Q

N

A

T
x
L

R

Q

F

F

T

M

L

M

A

A

Q

D

C

M

A

A

S

T

D

K

I

I

A

E

A

A

A

A

K

R

A

Y

F

L

L

V

N

Y

A

H

S

A

V

A

A

M

A

L

Y

K

E

N

R

E

G

G

E

K

L

P

T

Y

P

S

E

E

A

A

V

A

S

A

A

M

L

A

K

K

L

C

H

F

T

A

T

S

E

D

V

V

F

A

G

M

R

E

V

V

A

T

D

T

A

D

C

A

L

V

Q
|
Q

L
x
I

F

F

G

G

G
|
G

Y

Y

G

G

Y

Y

M

T

A

V

E

D

Y

Y

P

P

I

A

S

E

R

R

F

Y

W

M

T

R

D

N

A

A

R

K

V
x
I

L

T

R

Q

I

I

Y
|
Y

G
x
E

G
|
G

T
|
T

S

N

E
x
Q

I

V

M

M

K

R

E

I

L

V

V

T

A

S

R
|
R

S

A

L

L

L

L

active site: L125 (≠ M132), T126 (= T133), G242 (≠ E254), E363 (≠ G375), R375 (= R387)
binding coenzyme a persulfide: T132 (≠ S139), H179 (≠ K190), F232 (= F244), M236 (= M248), E237 (≠ R249), L239 (= L251), D240 (≠ P252), R243 (= R255), Y362 (= Y374), E363 (≠ G375), G364 (= G376), R375 (= R387)
binding flavin-adenine dinucleotide: F123 (≠ I130), L125 (≠ M132), T126 (= T133), G131 (= G138), T132 (≠ S139), F156 (= F167), I157 (= I168), T158 (≠ S169), R268 (= R280), Q270 (≠ A282), F271 (= F283), I275 (= I287), F278 (= F290), L281 (≠ T293), Q336 (= Q348), I337 (≠ L349), G340 (= G352), I358 (≠ V370), Y362 (= Y374), T365 (= T377), Q367 (≠ E379)

Sites not aligning to the query:

binding 1,3-propandiol: 5, 10

5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
38% identity, 93% coverage: 25:390/392 of query aligns to 18:377/378 of 5ol2F

query
sites
5ol2F

F

F

C

A

D

E

T

N

E

E

I

V

E

K

P

P

H

L

Y

A

R

T

D
x
E

W

L

E

D

K

E

A
x
E

G

E

L
x
R

V

F

S

P

R

Y

E

E

L

T

F

V

R

E

K

K

M

M

G

A

E

K

N

A

G

G

Y

M

L

M

C

G

A

I

D

P

V

Y

P

P

E

K

P

E

Y

Y

G

G

G

G

A

E

G

G

A

G

S

D

V

T

H

V

F

G

S

Y

F

I

A

M

V

A

V

V

E

E

V

E

L

L

S

S

R

R

R

V

-

C

G

G

Y

T

G

T

G

G

F

V

V

I

G

-

G

-

L

L

Q

S

V

A

H

H

N

T

D

S

I

L

I

G

P

S

P

W

Y

P

L

I

L

Y

H

Q

C

Y

G

G

T

N

E

E

A

E

Q

Q

R

K

Q

Q

Y

K

W

F

L

L

P

R

R

P

M

L

V

A

S

S

G

G

E

E

A

K

V

L

A

G

A

A

I
x
F

G

G

M
x
L

T
|
T

E

E

P
|
P

G

N

A

A

G

G

S
x
T

D

D

L

A

K

S

A

G

I

Q

R

Q

T

T

T

T

A

A

R

V

R

L

I

-

S

-

D

-

S

-

D

D

G

G

D

D

G

E

Y

Y

V

I

I

L

N

N

G

G

S

S

K

K

I

I

F
|
F

I
|
I

S
x
T

N

N

G

A

Q

I

H

A

C

G

D

D

L

I

L

Y

V

V

L

V

A

M

A

A

K

M

T

T

D

D

P

K

A

S

A

K

G

G

A

N

K

K

G

G

V

I

S

S

L

A

F

F

L

I

V

V

D

E

T

K

K

G

S

T

P

P

G

G

F

F

T

S

R

F

G

G

Q

V

N

K

L
x
E

E

K

K

K

I

M

G

G

Q

I

H

R

A

G

G

S

D

A

T

T

S

S

E

E

L

L

F

I

F

F

N

E

D

D

L

C

R

R

V

I

P

P

Q

K

D

E

A

N

L

L

L

L

G

G

G

K

V

-

E

E

G

G

Q

Q

G

G

F

F

V

K

Q

I

M

A

M

M

R

S

E

T

L
|
L

P

D

R

G

E
x
G

R
|
R

L

I

I

G

I

I

G

A

V

A

Q

Q

A

A

V

L

Y

G

G

L

A

A

K

Q

G

G

A

A

L

L

D

D

A

E

T

T

V

V

K

K

Y

Y

V

V

Q

K

E

E

R
|
R

Q

V

A

Q

F
|
F

G

G

Q

R

A

P

I
x
L

G

S

Q

K

F
|
F

Q

Q

N

N

T

T

R

Q

F

F

T

Q

L

L

A

A

Q

D

C

M

A

E

S

V

D

K

I

V

A

Q

A

A

A

A

K

R

A

H

F

L

L

V

N

Y

A

Q

S

A

V

A

A

I

A

N

Y

K

E

D

R

L

G

G

E

K

L

P

T

Y

P

G

E

V

A

E

V

A

S

A

A

M

L

A

K

K

L

L

H

F

T

A

T

A

E

E

V

T

F

A

G

M

R

E

V

V

A

T

D

T

A

K

C

A

L

V

Q
|
Q

L
|
L

F

H

G
|
G

G
|
G

Y

Y

G

G

Y

Y

M

T

A

R

E

D

Y

Y

P

P

I

V

S

E

R

R

F

M

W

M

T

R

D

D

A

A

R

K

V

I

L

T

R

E

I

I

Y
|
Y

G
x
E

G
|
G

T
|
T

S

S

E
|
E

I

V

M

Q

K

R

E

M

L

V

V

I

A

S

R
x
G

S

K

L

L

L

L

active site: L124 (≠ M132), T125 (= T133), G241 (≠ E254), G374 (≠ R387)
binding calcium ion: E29 (≠ D36), E33 (≠ A40), R35 (≠ L42)
binding coenzyme a persulfide: L238 (= L251), R242 (= R255), E362 (≠ G375), G363 (= G376)
binding flavin-adenine dinucleotide: F122 (≠ I130), L124 (≠ M132), T125 (= T133), P127 (= P135), T131 (≠ S139), F155 (= F167), I156 (= I168), T157 (≠ S169), E198 (≠ L210), R267 (= R280), F270 (= F283), L274 (≠ I287), F277 (= F290), Q335 (= Q348), L336 (= L349), G338 (= G351), G339 (= G352), Y361 (= Y374), T364 (= T377), E366 (= E379)

3r7kA Crystal structure of a probable acyl coa dehydrogenase from mycobacterium abscessus atcc 19977 / dsm 44196 (see paper)
37% identity, 99% coverage: 3:389/392 of query aligns to 1:377/378 of 3r7kA

query
sites
3r7kA

P

P

N

E

T

A

L

W

L

T

T

T

A

P

E

E

Q

R

K

R

A

A

E

L

L

S

Q

Q

V

M

F

A

T

R

A

S

S

F

L

V

E

E

R

R

F

-

C

-

D

-

T

-

E

E

I

I

E

A

P

P

H

K

Y

L

R

A

D

E

W

W

E

E

K

H

A

V

G

G

L

E

V

I

S

P

R

R

E

D

L

L

F

H

R

L

K

N

M

A

G

A

E

E

N

V

G

G

Y

L

L

L

C

G

A

I

D

G

V

F

P

P

E

E

P

E

Y

V

G

G

G

G

A

S

G

G

A

G

S

N

V

A

H

I

F

D

S

S

F

A

A

L

V

V

V

T

E

E

-

A

V

I

L

L

S

A

R

A

R

G

G

G

Y

S

G

T

G

G

F

V

V

C

G

A

G

A

L

L

Q

F

V

T

H

H

N

G

D

-

I

I

I

A

P

L

P

P

Y

H

L

I

L

A

H

A

C

N

G

G

T

S

E

D

A

A

Q

L

R

I

Q

E

Y

R

W

Y

L

V

P

R

R

P

M

T

V

L

S

A

G

G

E

K

A

M

V

I

A

G

A

S

I

L

G

G

M
x
V

T
|
T

E

E

P

P

G

G

A

A

G
|
G

S
|
S

D

D

L

V

K

A

A

N

I

L

R

R

T

T

T

R

A

A

R

V

R

R

I

-

S

-

D

-

S

-

D

E

G

G

D

D

G

T

Y

Y

V

V

I

V

N

N

G

G

S

A

K

K

I

T

F
|
F

I
|
I

S
x
T

N

S

G

G

Q

V

H

R

C

A

D

D

L

F

L

V

V

T

L

T

A

A

A

V

K

R

T

T

D

G

P

-

A

G

A

P

G

G

A

Y

K

G

G

G

V

V

S

S

L

L

F

L

L

V

V

I

D

D

T

K

K

N

S

S

P

P

G

G

F

F

T

E

R

V

G

S

Q

R

N

R

L

L

E

D

K

K

I

M

G

G

Q

W

H

R

A

C

G

S

D

D

T

T

S

A

E

E

L

L

F

S

F

F

N

V

D

D

L

V

R

R

V

V

P

P

Q

A

D

D

A

N

L

L

L

V

G

G

G

A

V

-

E

E

G

N

Q

S

G

G

F

F

V

L

Q

Q

M

I

M

M

R

Q

E

Q

L

F

P

Q

R

A

E
|
E

R

R

L

L

I

G

I

I

G

A

V

V

Q

Q

A

A

V

Y

Y

A

G

T

A

A

K

G

G

R

A

A

L

L

D

D

A

L

T

A

V

K

K

S

Y

W

V

A

Q

R

E

E

R
|
R

Q

E

A
x
T

F
|
F

G

G

Q

R

A

P

I
x
L

G

T

Q

G

F
x
R

Q

Q

N

I

T
x
I

R

R

F

H

T

K

L

L

A

A

Q

E

C

M

A

A

S

R

D

Q

I

V

A

D

A

V

A

A

K

C

A

T

F

Y

L

T

N

R

A

A

S

V

V

M

A

Q

A

R

Y

W

E

L

R

A

G

G

E

E

L

D

T

V

P

V

E

A

A

E

V

V

S

S

A

M

L

A

K

K

L

N

H

T

T

A

T

V

E

Y

V

A

F

C

G

D

R

Y

V

V

A

V

D

N

A

E

C

A

L

V

Q
|
Q

L
x
I

F

F

G

G

G
|
G

Y

M

G

G

Y

Y

M

M

A

R

E

E

Y

S

P

E

I

I

S

E

R

R

F

H

W

Y

T

R

D

D

A

C

R

R

V
x
I

L

L

R

G

I

I

Y

G

G
|
G

G

G

T
|
T

S

N

E
|
E

I

I

M

M

K

N

E

E

L

V

V

I

A

A

R
x
K

S

R

L

I

active site: V126 (≠ M132), T127 (= T133), E242 (= E254), G363 (= G375), K375 (≠ R387)
binding dihydroflavine-adenine dinucleotide: V126 (≠ M132), T127 (= T133), G132 (= G138), S133 (= S139), F157 (= F167), I158 (= I168), T159 (≠ S169), R268 (= R280), T270 (≠ A282), F271 (= F283), L275 (≠ I287), R278 (≠ F290), I281 (≠ T293), Q336 (= Q348), I337 (≠ L349), G340 (= G352), I358 (≠ V370), T365 (= T377), E367 (= E379)

5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
37% identity, 96% coverage: 16:391/392 of query aligns to 6:374/374 of 5lnxD

query
sites
5lnxD

Q

Q

V

V

F

M

T

M

A

R

S

K

L

M

E

V

R

R

-

D

F

F

C

A

D

R

T

K

E

E

I

I

E

A

P

P

H

A

Y

A

R

E

D

I

W

M

E

E

K

K

A

T

G

D

L

E

V

F

S

P

R

F

E

Q

L

L

F

I

R

K

K

K

M

M

G

G

E

K

N

H

G

G

Y

L

L

M

C

G

A

I

D

P

V

V

P

P

E

E

P

Q

Y

Y

G

G

G

G

A

A

G

G

A

A

S

D

V

V

-

V

H

S

F

Y

S

I

F

L

A

A

V

I

V

H

E

E

V

I

L

S

S

R

R

I

R

S

G

A

Y

A

G

V

G

G

F

V

V

I

G

-

G

-

L

L

Q

S

V

V

H

H

N

T

D

S

I

V

I

G

P

T

P

N

Y

P

L

I

L

L

H

Y

C

F

G

G

T

N

E

E

A

E

Q

Q

R

K

Q

M

Y

K

W

Y

L

I

P

P

R

N

M

L

V

A

S

S

G

G

E

D

A

H

V

L

A

G

A

A

I

F

G

A

M
x
L

T
|
T

E

E

P

P

G

H

A

S

G
|
G

S
|
S

D

D

L

A

K

G

A

S

I

L

R

R

T

T

T

T

A

A

R

I

R

K

I

K

S

N

D

G

S

K

D

-

G

-

D

-

G

-

Y

Y

V

L

I

L

N

N

G

G

S

S

K

K

I

I

F
|
F

I

I

S
x
T

N

N

G

G

Q

G

H

A

C

A

D

D

L

I

L

Y

V

I

L

T

A

F

A

A

K

L

T

T

D

A

P

P

A

D

A

Q

G

G

A

R

K

H

G

G

V

I

S

S

L

A

F

F

L

I

V

V

D

E

T

K

K

N

S

T

P

P

G

G

F

F

T

T

R

V

G

G

Q

K

N

K

L

E

E

R

K

K

I

L

G

G

Q

L

H

Y

A

G

G

S

D

N

T

T

S

T

E

E

L

L

F

I

F

F

N

D

D

N

L

A

R

E

V

V

P

P

Q

E

D

A

A

N

L

L

L

L

G

G

G

K

V

-

E

E

G

G

Q

D

G

G

F

F

V

H

Q

I

M

A

M

M

R

A

E

N

L

L

P

N

R

V

E
x
G

R

R

L

I

I

G

I

I

G

A

V

A

Q

Q

A

A

V

L

Y

G

G

I

A

A

K

E

G

A

A

A

L

L

D

E

A

H

T

A

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V

K

D

Y

Y

V

A

Q

K

E

Q

R
|
R

Q

V

A
x
Q

F
|
F

G

G

Q

R

A

P

I
|
I

G

A

Q

A

F
x
N

Q

Q

N

G

T
x
I

R

S

F

F

T

K

L

L

A

A

Q

D

C

M

A

A

S

T

D

R

I

A

A

E

A

A

A

A

K

R

A

H

F

L

L

V

N

Y

A

H

S

A

V

A

A

D

A

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Y

H

E

N

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R

G

L

E

N

L

C

T

G

P

K

E

E

A

A

V

-

S

S

A

M

L

A

K

K

L

Q

H

F

T

A

T

S

E

D

V

A

F

A

G

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R

K

V

A

A

L

D

D

A

A

C

-

L

V

Q
|
Q

L
x
I

F

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G

G

G
|
G

Y

Y

G

G

Y

Y

M

M

A

K

E

D

Y

Y

P

P

I

V

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E

R

R

F

L

W

L

T

R

D

D

A

A

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L

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R

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I

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Y
|
Y

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x
E

G

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T

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E
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E

I

I

M

Q

K

R

E

L

L

I

V

I

A

S

R
x
K

S

Y

L

L

L

L

G

G

active site: L122 (≠ M132), T123 (= T133), G239 (≠ E254), E358 (≠ G375), K370 (≠ R387)
binding flavin-adenine dinucleotide: L122 (≠ M132), T123 (= T133), G128 (= G138), S129 (= S139), F153 (= F167), T155 (≠ S169), R265 (= R280), Q267 (≠ A282), F268 (= F283), I272 (= I287), N275 (≠ F290), I278 (≠ T293), Q331 (= Q348), I332 (≠ L349), G335 (= G352), Y357 (= Y374), T360 (= T377), E362 (= E379)

4ktoA Crystal structure of a putative isovaleryl-coa dehydrogenase (psi- nysgrc-012251) from sinorhizobium meliloti 1021
37% identity, 96% coverage: 14:390/392 of query aligns to 13:375/377 of 4ktoA

query
sites
4ktoA

E

E

L

I

Q

D

V

A

F

L

T

R

A

A

S

S

L

V

E

R

R

R

F

F

C

A

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T

E

E

R

I

I

E

A

P

P

H

L

Y

A

R

D

D

D

W

A

E

D

K

R

A

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N

L

A

V

F

S

P

R

M

E

S

L

L

F

W

R

R

K

E

M

M

G

G

E

E

N

L

G

G

Y

L

L

L

C

G

A

I

D

T

V

A

P

D

E

E

P

A

Y

H

G

G

G

G

A

A

G

G

A

L

S

G

V

Y

H

L

F

A

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H

F

C

A

V

V

A

V

M

E

E

V

E

L

I

S

S

R

R

R

A

G

S

Y

A

G

S

-

V

G

G

F

L

V

S

G

Y

G

G

L

-

Q

-

V

A

H

H

N

S

D

N

I

L

I

C

P

V

P

N

Y

Q

L

I

L

N

H

R

C

N

G

G

T

K

E

P

A

A

Q

Q

R

K

Q

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Y

R

W

Y

L

L

P

P

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K

M

L

V

I

S

S

G

G

E

E

A

H

V

V

A

G

A

A

I
x
L

G

A

M
|
M

T
x
S

E

E

P

P

G

G

A

V

G

S

S

M

D

K

L

L

K

K

A

A

I

-

R

-

T

-

T

-

A

-

R

-

R

-

I

-

S

-

D

D

S

K

D

R

G

G

D

D

G

R

Y

Y

V

V

I

L

N

N

G

G

S

S

K

K

I
x
M

F
x
W

I

I

S
x
T

N

N

G

G

Q

P

H

D

C

A

D

D

L

V

L

L

V

V

L

V

A

Y

A

A

K

K

T

T

D

D

P

P

A

P

A

-

G

-

A

-

K

R

G

G

V

I

S

T

L

A

F

F

L

L

V

V

D

E

T

K

K

A

S

F

P

P

G

G

F

F

T

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R

A

G

G

Q

Q

N

K

L

L

E

D

K

K

I

L

G

G

Q

M

H

R

A

G

G

S

D

N

T

T

S

S

E

E

L

L

F

I

F

F

N

T

D

D

L

C

R

E

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V

P

P

Q

E

D

E

A

N

L

V

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L

G

G

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-

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G

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G

G

F

V

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G

L

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E
|
E

R

R

L

V

I

V

I

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S

V

A

Q

G

A

P

V

L

Y

G

G

I

A

M

K

A

G

A

A

C

L

L

D

D

A

V

T

V

V

V

K

P

Y

Y

V

L

Q

H

E

E

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|
R

Q

K

A

Q

F
|
F

G

G

Q

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A

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I
|
I

G

G

Q

E

F
|
F

Q

Q

N

L

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x
M

R

Q

F

G

T

K

L

L

A

A

Q

D

C

M

A

Y

S

V

D

T

I

M

A

N

A

A

A

A

K

R

A

A

F

Y

L

V

N

Y

A

A

S

V

V

A

A

A

A

A

Y

C

E

D

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R

G

G

E

E

L

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T

A

P

R

E

K

A

D

V

A

S

A

A

G

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C

K

I

L

L

H

Y

T

A

T

A

E

E

V

K

F

A

G

T

R

A

V

M

A

A

D

L

A

E

C

A

L

I

Q
|
Q

L
x
A

F

L

G

G

G
|
G

Y

N

G

G

Y

Y

M

T

A

N

E

D

Y

Y

P

P

I

A

S

G

R

R

F

L

W

L

T

R

D

D

A

A

R

K

V
x
L

L

Y

R

E

I

I

Y
x
G

G
x
A

G

G

T
|
T

S

S

E
|
E

I

I

M

R

K

R

E

M

L

L

V

I

A

G

R
|
R

S

E

L

L

L

F

active site: M130 (= M132), S131 (≠ T133), E239 (= E254), A360 (≠ G375), R372 (= R387)
binding flavin-adenine dinucleotide: L128 (≠ I130), M130 (= M132), S131 (≠ T133), M155 (≠ I166), W156 (≠ F167), T158 (≠ S169), R265 (= R280), F268 (= F283), I272 (= I287), F275 (= F290), M278 (≠ T293), Q333 (= Q348), A334 (≠ L349), G337 (= G352), L355 (≠ V370), G359 (≠ Y374), T362 (= T377), E364 (= E379)

P15651 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Rattus norvegicus (Rat) (see 2 papers)
35% identity, 98% coverage: 6:390/392 of query aligns to 25:407/412 of P15651

query
sites
P15651

L

L

L

H

T

T

A

V

E

Y

Q

Q

K

S

A

V

E

E

L

L

-

P

-

E

-

T

-

H

Q

Q

V

M

F

L

T

R

A

Q

S

T

L

C

E

R

R

D

F

F

C

A

D

E

T

K

E

E

I

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E

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P

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I

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A

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A

D

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E

D

K

K

A

E

G

H

L

L

V

F

S

P

R

T

E

S

L

Q

F

V

R

K

K

K

M

M

G

G

E

E

N

L

G

G

Y

L

L

L

C

A

A

M

D

D

V

V

P

P

E

E

P

E

Y

L

G

S

G

G

A

A

G

G

A

L

S

D

-

Y

V

L

H

A

F

Y

S

S

F

I

A

A

V

L

V

E

E

E

V

I

L

-

S

-

R

S

R

R

G

G

Y

C

G

A

G

S

F

T

V

G

G

V

G

I

L

M

Q

S

V

V

H

N

N

N

D

S

I

L