SitesBLAST
Comparing WP_054257276.1 NCBI__GCF_001298675.1:WP_054257276.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
49% identity, 99% coverage: 4:470/472 of query aligns to 6:465/465 of 3pm9A
- active site: A149 (= A148), L159 (≠ N158)
- binding flavin-adenine dinucleotide: P69 (= P66), Q70 (= Q67), G71 (= G68), G72 (= G69), N73 (= N70), T74 (= T71), G75 (= G72), L76 (= L73), G79 (= G76), Q80 (≠ S77), L91 (= L90), L133 (= L132), G134 (≠ A133), A135 (= A134), C139 (= C138), T140 (= T139), G142 (= G141), G143 (= G142), S146 (≠ G145), T147 (= T146), A149 (= A148), G150 (= G149), E200 (= E199), G201 (= G200), I205 (≠ V204), I206 (= I205), E423 (= E428)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
39% identity, 95% coverage: 23:470/472 of query aligns to 32:464/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R331), T337 (≠ P335), K348 (= K346), Y379 (≠ F379), H381 (= H381), H388 (= H388), H423 (= H429)
- binding flavin-adenine dinucleotide: W39 (= W30), P75 (= P66), Q76 (= Q67), G77 (= G68), G78 (= G69), N79 (= N70), T80 (= T71), G81 (= G72), M82 (≠ L73), G85 (= G76), S86 (= S77), L139 (= L132), G140 (≠ A133), A141 (= A134), C145 (= C138), G149 (= G142), N150 (= N143), A152 (≠ G145), T153 (= T146), G157 (= G150), G207 (= G200), I212 (= I205), E422 (= E428), N459 (= N465)
- binding zinc ion: H381 (= H381), H388 (= H388), E422 (= E428)
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
39% identity, 95% coverage: 23:470/472 of query aligns to 32:464/466 of 6lpuA
- binding flavin-adenine dinucleotide: W39 (= W30), P75 (= P66), G77 (= G68), G78 (= G69), N79 (= N70), T80 (= T71), G81 (= G72), G85 (= G76), S86 (= S77), L139 (= L132), G140 (≠ A133), A141 (= A134), C145 (= C138), H146 (≠ T139), G148 (= G141), G149 (= G142), N150 (= N143), A152 (≠ G145), T153 (= T146), A155 (= A148), E206 (= E199), G207 (= G200), I211 (≠ V204), I212 (= I205), E422 (= E428), N459 (= N465)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R331), T337 (≠ P335), K348 (= K346), Y379 (≠ F379), H381 (= H381), H388 (= H388), H423 (= H429)
- binding zinc ion: H381 (= H381), H388 (= H388), E422 (= E428)
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
39% identity, 95% coverage: 23:470/472 of query aligns to 32:464/466 of 6lpqA
- binding flavin-adenine dinucleotide: W39 (= W30), P75 (= P66), G77 (= G68), G78 (= G69), N79 (= N70), T80 (= T71), G81 (= G72), G85 (= G76), S86 (= S77), L139 (= L132), G140 (≠ A133), A141 (= A134), C145 (= C138), H146 (≠ T139), G149 (= G142), N150 (= N143), A152 (≠ G145), T153 (= T146), A155 (= A148), G157 (= G150), E206 (= E199), G207 (= G200), I211 (≠ V204), I212 (= I205), E422 (= E428), N459 (= N465)
- binding d-malate: M82 (≠ L73), R333 (= R331), T337 (≠ P335), K348 (= K346), Y379 (≠ F379), H381 (= H381), H388 (= H388), E422 (= E428), H423 (= H429)
- binding zinc ion: H381 (= H381), H388 (= H388), E422 (= E428)
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
39% identity, 95% coverage: 23:470/472 of query aligns to 32:464/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R331), T337 (≠ P335), K348 (= K346), Y379 (≠ F379), H381 (= H381), H388 (= H388), N390 (= N390), H423 (= H429)
- binding flavin-adenine dinucleotide: W39 (= W30), P75 (= P66), G77 (= G68), G78 (= G69), N79 (= N70), T80 (= T71), G81 (= G72), M82 (≠ L73), G85 (= G76), S86 (= S77), L139 (= L132), G140 (≠ A133), A141 (= A134), C145 (= C138), G149 (= G142), N150 (= N143), A152 (≠ G145), T153 (= T146), A155 (= A148), G157 (= G150), G207 (= G200), I212 (= I205), E422 (= E428), H423 (= H429)
- binding zinc ion: H381 (= H381), H388 (= H388), E422 (= E428)
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
39% identity, 95% coverage: 23:470/472 of query aligns to 33:465/467 of 6lpnB
- binding flavin-adenine dinucleotide: W40 (= W30), P76 (= P66), G78 (= G68), G79 (= G69), N80 (= N70), T81 (= T71), G82 (= G72), M83 (≠ L73), G86 (= G76), S87 (= S77), L140 (= L132), A142 (= A134), C146 (= C138), H147 (≠ T139), G150 (= G142), N151 (= N143), A153 (≠ G145), T154 (= T146), G208 (= G200), I212 (≠ V204), I213 (= I205), E423 (= E428), N460 (= N465)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
39% identity, 95% coverage: 23:470/472 of query aligns to 85:517/521 of Q8N465
- S109 (≠ T47) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ V65) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G69) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ V87) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ L93) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (= C112) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (= P129) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (≠ G145) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ P171) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G173) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ S320) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R331) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- T390 (≠ P335) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ N344) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (= K346) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- R419 (≠ L367) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ V374) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H381) binding Zn(2+); mutation to A: Loss of catalytic activity.
- G436 (= G383) to V: slight reduction in catalytic activity
- N439 (= N386) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H388) binding Zn(2+); mutation to A: Loss of catalytic activity.
- N443 (= N390) binding (R)-2-hydroxyglutarate; mutation to A: Significantly reduced catalytic activity.
- V444 (= V391) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (= A399) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E428) binding Zn(2+); mutation to A: Loss of catalytic activity.
- H476 (= H429) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- G477 (= G430) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
32% identity, 96% coverage: 19:470/472 of query aligns to 49:496/496 of P39976
Sites not aligning to the query:
- 17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
31% identity, 91% coverage: 41:471/472 of query aligns to 48:465/467 of 7qh2C