SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_054342151.1 NCBI__GCF_001305295.1:WP_054342151.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6vw7D Formate dehydrogenase fdsabg subcomplex fdsbg from c. Necator - nadh bound (see paper)
65% identity, 97% coverage: 5:522/533 of query aligns to 7:513/514 of 6vw7D

query
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6vw7D

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binding flavin mononucleotide: G154 (= G163), G156 (= G165), K165 (= K174), N182 (= N191), E185 (= E194), G273 (= G282), E274 (= E283), E275 (= E284), N309 (= N318), N310 (= N319), S313 (= S322), A490 (= A499), M491 (= M500)
binding 1,4-dihydronicotinamide adenine dinucleotide: A158 (= A167), F160 (= F169), K165 (= K174), T168 (= T177), E275 (= E284), L295 (= L304)
binding iron/sulfur cluster: V271 (≠ I280), V289 (= V298), S442 (= S451), C443 (= C452), G444 (= G453), K445 (= K454), C446 (= C455), C449 (= C458), L488 (= L497), C489 (= C498), M491 (= M500), G492 (= G501)

6tg9B Cryo-em structure of nadh reduced form of NAD+-dependent formate dehydrogenase from rhodobacter capsulatus (see paper)
57% identity, 97% coverage: 3:519/533 of query aligns to 1:492/493 of 6tg9B

query
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6tg9B

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binding flavin mononucleotide: G146 (= G163), R147 (= R164), G148 (= G165), N174 (= N191), D176 (= D193), E177 (= E194), Y254 (= Y279), G257 (= G282), E258 (= E283), N293 (= N318), N294 (= N319), S297 (= S322)
binding 1,4-dihydronicotinamide adenine dinucleotide: F152 (= F169), K157 (= K174), E258 (= E283), E259 (= E284), L279 (= L304), K466 (≠ I493), L470 (= L497)
binding iron/sulfur cluster: S426 (= S451), C427 (= C452), G428 (= G453), T429 (≠ K454), C430 (= C455), C433 (= C458), L470 (= L497), C471 (= C498), G474 (= G501)

8oh5B Cryo-em structure of the electron bifurcating transhydrogenase stnabc complex from sporomusa ovata (state 2) (see paper)
42% identity, 97% coverage: 3:518/533 of query aligns to 1:508/584 of 8oh5B

query
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8oh5B

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A

F

F

A

M

D

D

R

R

L

S

V

V

M

L

E

E

A

G

D

D

P

P

L

H

M

A

L

L

V

L

E

E

G

G

M

M

I

A

I

I

A

C

G

G

V

Y

A

A

V

I

D

G

A

A

T

N

Q

E

G

G

Y

H

I

I

Y

Y

L

C

R

R

S

A

E

E

Y

Y

P

P

V

L

A

A

Y

I

Q

K

I

R

M

L

N

E

E

I

A

A

I

I

A

A

N

D

A

A

Y

K

A

Q

A

R

G

N

Y

L

L

L

G

G

E

K

H

N

I

I

L

M

G

G

S

T

D

N

K

F

T

S

F

F

H

D

L

M

E

K

V

I

R

K

L

K

G

G

A

A

A

G

A

A

Y
x
F

I

V

C

C

G

G

E
|
E

T

T

S

A

L

L

L

I

E

A

S

S

L

L

E

E

G

G

K

E

R

R

G

G

M

M

V
x
P

R

R

F

L

K
|
K

P

P

L
x
F

P

P

A

A

I

Q

E

S

G

G

L

F

F

W

G

G

K

K

P

P

T

T

V

N

V
x
I

N
|
N

V

V

L

E

S

T

L

F

A

A

A

N

I

V

P

P

F

W

I

I

M

M

E

Y

K

N

G

G

S

G

Q

S

A

A

Y

Y

A

A

D

A

Y

Y

G

G

M

T

G

E

R

K

S

S

R

K

G

G

T

T

L

K

P

V

F

F

Q

A

L

L

T

A

G

G

N

K

L

I

K

K

Q

N

G

G

L

L

V

V

E

E

L

V

A

P

F

M

G

G

P

M

T

S

L

L

R

R

E

E

L

V

V

I

D

Y

N

D

F

I

G

G

T

I

E

L

T

N

G

D

L

R

P

E

M

F

R

K

A

A

I

V

Q

Q

V

M

G

G

P

P

L

S

G

G

A

G

Y

C

L

I

P

P

A

K

S

Q

Q

L

W

L

D

D

T

T

P

P

L

V

D

D

Y

Y

E

D

A

S

F

I

A

N

A

K

A

T

G

G

A

A

V

I

L

M

G
|
G

H

S

G

G

V

M

V

I

V

V

F

M

D

D

E

E

S

T

V

T

D
x
C

M

M

T

V

E

D

Q

M

A

A

R

R

F

F

S

F

M

L

E

D

F

F

C

T

V

V

A

K

E

E

S

S

C
|
C

G
|
G

K

K

C
|
C

T

I

P

Y

C
|
C

R

R

I

I

G

G

S

T

T

K

R

R

G

M

V

L

E

E

V

I

I

L

D

E

K

R

I

I

R

T

S

T

G

G

N

E

N

G

V

R

A

E

A

G

N

D

L

I

A

E

L

E

L

L

D

E

L

L

C

S

D

I

T

S

M

I

I

K

D

D

G

G

S

S

L

L

C
|
C

A
x
G

M
x
L

G
|
G

S

Q

M

T

T

A

P

P

I

N

P

P

V

V

K

L

S

T

A

T

F

I

K

R

Y

Y

F

F

S

R

E

D

D

E

binding fe2/s2 (inorganic) cluster: C10 (≠ T12), C41 (≠ S43), C45 (≠ F47)
binding flavin mononucleotide: R154 (= R164), K164 (= K174), N181 (= N191), F269 (≠ Y279), E273 (= E283), E274 (= E284), I307 (≠ V317), N308 (= N318), N309 (= N319), G489 (≠ A499), L490 (≠ M500)
binding nicotinamide-adenine-dinucleotide: G155 (= G165), G156 (= G166), F159 (= F169), F163 (≠ I173), E273 (= E283), E274 (= E284), K291 (= K301), F294 (≠ L304), G413 (= G423)
binding iron/sulfur cluster: P288 (≠ V298), C442 (= C452), G443 (= G453), C445 (= C455), C448 (= C458), C488 (= C498), L490 (≠ M500), G491 (= G501)
binding zinc ion: C425 (≠ D435)

Sites not aligning to the query:

binding iron/sulfur cluster: 531, 536, 540, 542, 546, 547, 548, 559, 566, 567, 568, 569, 572
binding zinc ion: 512, 518, 523

7q4vB Electron bifurcating hydrogenase - hydabc from a. Woodii (see paper)
42% identity, 97% coverage: 4:518/533 of query aligns to 7:523/599 of 7q4vB

query
sites
7q4vB

K

Q

I

I

F

L

L

I

P
x
C

R

G

D

G

T

T

T

G

T
x
C

L

T

A

S

L

S

G

G

G

S

E

M

K

V

V

L

V

V

K

K

A

E

I

L

R

K

T

K

E

E

A

L

D

V

V

K

R

H

G

D

L

I

-

L

-

D

E

E

I

V

E

E

L

V

V

V

R

T

N

T

G

G

S
x
C

R
x
F

G

G

L
|
L

F
x
C

W

E

L

L

E

G

P

P

M

V

L

V

E

I

V

V

Q

Y

T

P

Q

E

N

G

G

-

R

-

I

T

A

F

Y

Y

G

S

P

R

V

V

E

E

A

A

S

A

D

D

V

I

A

P

A

E

I

M

F

V

D

E

E

E

I

H

F

L

N

V

P

K

G

G

-

R

-

P

L

L

D

D

S

R

E

L

L

I

S

Y

E

N

G

E

N

K

T

G

G

D

D

G

A

H

H

H

P

P

L

L

A

S

L

I

G

-

N

-

P

-

E

N

E

E

I

L

P

G

Y

F

L

F

K

K

N

K

Q

Q

Q

R

R

R

L

I

T

A

F

L

A

A

R

N

A

C

G

G

I

V

T

I

D

N

P

P

V

E

S

N

I

I

A

D

D

E

Y

Y

L

I

A

G

H

F

D

D

G

G

F

Y

K

L

G

A

L

L

E

E

N

K

S

V

L

L

-

L

A

T

L

M

N

S

S

P

Q

V

A

D

I

V

V

I

D

N

E

E

V

V

K

K

A

A

S

S

G

G

L

L

R

R

G
|
G

R

R

G
|
G

G

G

A

G

F

F

P

P

T

T

G

G

I

L

K

K

W

W

Q

Q

T

F

V

A

H

H

D

D

C

A

S

V

A

S

D

E

Q

D

-

G

-

I

K

K

Y

Y

I

V

V

A

C

C

N
|
N

A

A

D
|
D

E

E

G

G

D

D

S

P

G

G

T

A

F

F

A

M

D

D

R

R

L

S

V

V

M

L

E

E

A

G

D

D

P

P

L

H

M

A

L

V

V

I

E

E

G

A

M

M

I

A

I

I

A

A

G

G

V

Y

A

A

V

V

D

G

A

A

T

S

Q

K

G

G

Y

Y

I

V

Y

Y

L

V

R

R

S

A

E

E

Y

Y

P

P

V

I

A

A

Y

V

Q

N

I

R

M

L

N

Q

E

I

A

A

I

I

A

D

N

Q

A

A

Y

K

A

E

A

Y

G

G

Y

I

L

L

G

G

E

E

H

N

I

I

L

F

G

E

S

T

D

D

K

F

T

S

F

F

H

D

L

L

E

E

V

I

R

R

L

L

G

G

A

A

A

G

A

A

Y
x
F

I

V

C

C

G
|
G

E
|
E

T

T

S

A

L

L

L

M

E

N

S

S

L

I

E

E

G

G

K

K

R

R

G

G

M

E

V

P

R

R

F

P

K

R

P

P

L

F

P

P

A

A

I

N

E

K

G

G

L

L

F

F

G

G

K

K

P

P

T

T

V

V

V

L

N

N

N
|
N

V

V

L

E

S

T

L

Y

A

A

A

N

I

I

P

P

F

K

I

I

M

I

E

L

K

N

G

G

S

A

Q

E

A

W

Y

F

A

A

D

S

Y

V

G

G

M

T

G

E

R

K

S

S

R

K

G

G

T

T

L

K

P

V

F

F

Q

A

L

L

T

G

G

G

N

K

L

I

K

N

Q

N

G

T

G

G

L

L

V

L

E

E

L

I

A

P

F

M

G

G

P

T

T

T

L

L

R

R

E

E

L

I

V

I

D

Y

N

E

F

I

G

G

T

I

E

P

T

N

G

G

L

K

P

A

M

F

R

K

A

A

I

A

Q

Q

V

T

G

G

P

P

L

S

G

G

A

G

Y

C

L

L

P

P

A

E

S

S

Q

L

W

L

D

D

T

T

P

E

L

I

D

D

Y

Y

E

D

A

N

F

L

A

I

A

A

G

G

A

S

V

M

L

M

G

G

H

S

G

G

V

L

V

I

V

V

F

M

D

D

E

E

S

D

V

N

D
x
C

M

M

T

V

E

D

Q

V

A

A

R

R

F

F

S

F

M

L

E

D

F

F

C

T

V

Q

A

D

E

E

S

S

C
|
C

G
|
G

K
|
K

C
|
C

T

P

P

P

C
|
C

R

R

I

I

G

G

S

T

T

K

R

R

G

M

V

L

E

E

V

I

I

L

D

E

K

R

I

I

R

C

S

D

G

G

N

K

N

G

V

V

A

E

A

G

N

D

L

I

A

E

L

R

L

L

D

E

L

L

C

A

D

V

T

G

M

I

I

K

D

S

G

S

S

A

L

L

C
|
C

A

G

M

L

G
|
G

S

Q

M

T

T

A

P

P

I

N

P

P

V

V

K

L

S

S

A

T

F

I

K

R

Y

F

F

F

S

R

E

D

D

E

binding fe2/s2 (inorganic) cluster: C11 (≠ P8), C16 (≠ T13), C48 (≠ S43), F49 (≠ R44), L51 (= L46), C52 (≠ F47)
binding flavin mononucleotide: G166 (= G163), G168 (= G165), N196 (= N191), D198 (= D193), F284 (≠ Y279), G287 (= G282), E288 (= E283), E289 (= E284), N324 (= N319)
binding iron/sulfur cluster: C457 (= C452), G458 (= G453), K459 (= K454), C460 (= C455), C463 (= C458), C503 (= C498), G506 (= G501)
binding zinc ion: C440 (≠ D435)

Sites not aligning to the query:

binding iron/sulfur cluster: 544, 551, 552, 553, 554, 555, 556, 557, 561, 562, 574, 581, 583, 584, 585, 586, 587, 591, 593, 595
binding zinc ion: 527, 533

8a6tB Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from thermoanaerobacter kivui in the reduced state (see paper)
45% identity, 78% coverage: 105:518/533 of query aligns to 141:554/630 of 8a6tB

query
sites
8a6tB

E

D

E

E

I

N

P

P

Y

F

L

Y

K

A

N

K

Q

Q

Q

T

R

R

L

L

T

V

F

L

A

K

R

H

A

C

G

G

I

T

T

V

D

D

P

P

V

A

S

S

I

V

A

Y

D

D

Y

Y

L

I

A

A

H

E

D

G

G

G

F

Y

K

S

G

A

L

I

E

A

N

K

S

A

L

L

A

T

L

M

N

D

S

R

Q

K

A

Q

I

I

V

I

D

D

E

E

V

V

K

I

A

K

S

S

G

G

L

L

R

R

G

G

R

R

G
|
G

G

G

A

A

A

G

F

F

P

P

T

T

G

G

I

E

K

K

W

W

Q

L

T

G

V

A

H

Y

D

K

C

N

S

Q

A

S

D

P

Q

K

K

K

Y

Y

I

I

V

I

C

C

N
|
N

A

G

D

D

E
|
E

G

G

D

D

S

P

G

G

T

A

F

F

A

M

D

D

R

R

L

S

V

V

M

M

E

E

A

G

D

D

P

P

L

H

M

K

L

V

V

I

E

E

G

G

M

M

I

M

I

I

A

G

G

A

V

Y

A

A

V

I

D

G

A

S

T

D

Q

E

G

G

Y

Y

I

I

Y

Y

L

V

R

R

S

A

E

E

Y

Y

P

P

V

L

A

A

Y

V

Q

Q

I

M

M

L

N

R

E

K

A

A

I

I

A

E

N

E

A

C

Y

E

A

K

A

L

G

G

Y

L

L

L

G

G

E

D

H

N

I

I

L

L

G

G

S

T

D

G

K

F

T

S

F

F

H

R

L

L

E

H

V

V

R

R

L

E

G

G

A

A

A

G

A

A

Y

F

I

V

C

C

G

G

E

E

E
x
S

T

T

S

A

L

L

L

T

E

Y

S

S

L

I

E

E

G

G

K

K

R

R

G

G

M

M

V

P

R

R

F

V

K
x
R

P

P

L
x
R

P
x
T

A
x
N

I

E

E

C

G

G

L

L

F

W

G

E

K

M

P

P

T

T

V

V

V

L

N

N

N
|
N

V

V

L

E

S

T

L

F

A

A

A

C

I

I

P

P

F

E

I

I

M

I

E

L

K

N

G

G

S

G

Q

E

A

W

Y

F

A

A

D

S

Y

I

G

G

M

T

G

P

R

T

S

S

R

T

G

G

T

T

L

K

P

I

F

F

Q

A

L

L

T

S

G

G

N

K

L

V

K

N

Q

R

G

T

G

G

L

L

V

V

E

E

L

V

A

P

F

M

G

G

P

L

T

K

L

L

R

R

E

E

L

L

V

I

D

F

N

D

F

I

G

G

T

I

E

A

T

N

G

N

L

K

P

K

M

F

R

K

A

A

I

V

Q

Q

V

L

G

G

P

P

L
x
S

G

G

A

G

Y

C

L

V

P

P

A

E

S

S

Q

Q

W

L

D

D

T

L

P

P

L

I

D

D

Y

F

E

D

A

S

F

L

A

S

A

K

A

A

G

G

A

A

V

I

L

M

G

G

H

S

G

G

V

V

F

V

D

D

E

E

S

D

V

T

D
x
C

M

M

T

V

E

D

Q

F

A

A

R

K

F

F

S

F

M

T

E

N

F

F

C

I

V

V

A

E

E

E

S
|
S

C
|
C

G
|
G

K

K

C
|
C

T

I

P

P

C
|
C

R

R

I

E

G

G

S

N

T

K

R

K

G

M

V

L

E

E

V

I

I

L

D

E

K

R

I

I

R

T

S

E

G

G

N

K

N

G

V

K

A

E

A

G

N

D

L

I

A

E

L

L

D

E

L

L

C

G

D

D

T

V

M

I

I

I

D

S

G

A

S

S

L

L

C
|
C

A
x
G

M
x
L

G
|
G

S

K

M

T

T

A

P

P

I

N

P

P

V

V

K

L

S

S

A

T

F

I

K

K

Y

H

F

F

S

R

E

D

D

E

binding flavin mononucleotide: G201 (= G165), N227 (= N191), E230 (= E194), N355 (= N319), G535 (≠ A499), L536 (≠ M500)
binding nadp nicotinamide-adenine-dinucleotide phosphate: S320 (≠ E284), R337 (≠ K301), R340 (≠ L304), T341 (≠ P305), N342 (≠ A306), S433 (≠ L397)
binding iron/sulfur cluster: S487 (= S451), C488 (= C452), G489 (= G453), C491 (= C455), C494 (= C458), C534 (= C498), L536 (≠ M500), G537 (= G501)
binding zinc ion: C471 (≠ D435)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 31, 33, 36, 82, 85, 86
binding iron/sulfur cluster: 575, 577, 582, 583, 585, 588, 592, 596, 597, 607, 612, 618, 622, 624, 626, 627
binding zinc ion: 558, 564

7p8nB Tmhydabc- t. Maritima hydrogenase with bridge closed (see paper)
41% identity, 98% coverage: 3:523/533 of query aligns to 26:544/613 of 7p8nB

query
sites
7p8nB

M

V

K

S

I

I

F

Y

L

V

P
x
C

R

V

D
x
G

T
|
T

T

G

T
x
C

L

T

A

A

L

K

G

G

G

A

E

L

K

K

V

V

V

Y

K

S

A

A

I

F

R

E

T

E

E

E

A

L

D

K

V

K

R

R

G

N

L

L

-

L

E

K

I

V

E

T

L

L

V

N

R

R

N

T

G

G

S
x
C

R
x
C

G
|
G

L
x
R

F
x
C

W

S

L

S

E

G

P

P

M

L

L

V

E

K

V

I

Q

M

T

P

Q

Y

N

-

G

-

R

R

I

F

A

F

Y

Y

G

S

P

N

V

V

E

A

A

P

S

E

D

D

V

V

A

P

A

E

I

I

F

V

D

D

E

R

I

T

F

V

N

L

P

K

G

G

L

E

D

P

S

I

E

E

-

R

-

L

-

F

L

L

S

T

E

D

G

P

N

L

T

T

G

G

D

E

A

K

H

V

P

P

L

R

A

I

L

-

G

-

N

-

P

-

E

E

E

D

I

T

P

T

Y

L

L

F

K

K

N

N

Q

Q

Q

D

R

F

L

Y

T

I

F

M

A

E

R

A

A

I

G

G

I

E

T

S

D

E

P

C

V

D

S

S

I

I

A

E

D

D

Y

Y

L

I

A

A

H

R

D

S

G

G

F

Y

K

E

G

S

L

L

E

V

N

K

S

A

L

L

-

T

A

S

L

M

N

T

S

P

Q

E

A

E

I

I

V

I

D

E

E

T

V

V

K

K

A

A

S

S

G

G

L

L

R

R

G
|
G

R
|
R

G
|
G

G

G

A

G

F

F

P

P

T

T

G

G

I

L

K

K

W

W

Q

E

T

F

V

T

H

R

D

K

C

A

S

Q

A

G

D

D

Q

I

K

K

Y

F

I

V

V

V

C

C

N
|
N

A

G

D
|
D

E
|
E

G
|
G

D

D

S

P

G

G

T

A

F

F

A

M

D

N

R

R

L

T

V

L

M

L

E

E

A

R

D

D

P

P

L

H

M

L

L

V

V

L

E

E

G

G

M

M

I

I

A

A

G

G

V

Y

A

A

V

V

D

G

A

A

T

Q

Q

K

G

G

Y

Y

I

A

Y

Y

L

I

R

R

S

A

E

E

Y

Y

P

P

V

F

A

A

Y

V

Q

K

I

M

M

F

N

K

E

K

A

A

I

I

A

E

N

D

A

A

Y

R

A

K

A

L

G

G

Y

L

L

L

G

G

E

E

H

N

I

I

L

L

G

G

S

T

D

G

K

F

T

S

F

F

H

D

L

L

E

E

V

V

R

K

L

E

G

G

A

A

A

G

A

A

Y
x
F

I

V

C

C

G
|
G

E
|
E

T

T

S

A

L

L

E

A

S

S

L

I

E

E

G

G

K

K

R

R

G

G

M

M

V
x
P

R

R

F

P

K

K

P

P

L

F

P

P

A

A

I

Q

E

S

G

G

L

L

F

W

G

G

K

K

P

P

T

T

V

L

V

I

N
|
N

V

V

L

E

S

T

L

Y

A

A

A

N

I

I

P

P

F

R

I

I

M

L

E

R

K

D

G

G

S

V

Q

E

A

N

Y

Y

A

R

D

K

Y

R

G

G

M

T

G

E

R

N

S

S

R

P

G

G

T

T

L

K

P

M

F

F

Q

S

L

V

T

A

G

G

N

P

L

L

K

K

Q

A

G

T

G

G

L

I

V

I

E

E

L

V

A

E

F

F

G

G

P

T

T

T

L

L

R

R

E

D

L

I

V

I

D

Y

N

N

F

I

G

C

G

G

T

F

E

V

T

E

G

G

L

E

P

E

M

F

R

K

A

A

I

V

Q

Q

V

I

G

G

P

P

L

S

G

G

A

A

Y

C

L

L

P

S

A

E

S

D

Q

F

W

I

D

D

T

M

P

P

L

L

D

D

Y

Y

E

D

A

T

F

L

A

K

A

A

G

D

A

A

V

M

L

V

G

G

H

S

G

G

V

I

V

V

F

I

D

T

E

K

S

K

V

T

D
x
C

M

M

T

V

E

E

Q

V

A

A

R

R

F

F

S

F

M

L

E

D

F

F

C

T

V

K

A

R

E

E

S
|
S

C
|
C

G
|
G

K
|
K

C
|
C

T

V

P

P

C
|
C

R

R

I

E

G

G

S

T

T

M

R

Q

G

A

V

Y

E

N

V

I

I

L

D

E

K

K

I

F

R

T

S

H

G

G

N

K

N

A

V

T

A

Y

A

E

N

D

L

L

A

K

L

T

L

L

D

E

D

H

L

L

C

S

D

K

T

T

M

I

I

K

D

T

G

A

S

S

L
|
L

C
|
C

A
x
G

M
x
L

G
|
G

S

K

M

T

T

A

P

P

I

N

P

P

V

I

K

L

S

S

A

T

F

L

K

K

Y

L

F

F

S

R

E

E

D

E

I

Y

A

-

L

I

A

A

H
|
H

binding fe2/s2 (inorganic) cluster: C31 (≠ P8), G33 (≠ D10), T34 (= T11), C36 (≠ T13), C67 (≠ S43), C68 (≠ R44), G69 (= G45), R70 (≠ L46), C71 (≠ F47)
binding flavin mononucleotide: G185 (= G163), R186 (= R164), G187 (= G165), N213 (= N191), D215 (= D193), E216 (= E194), G217 (= G195), F301 (≠ Y279), G304 (= G282), E305 (= E283), E306 (= E284), N340 (= N318), N341 (= N319), G521 (≠ A499), L522 (≠ M500)
binding iron/sulfur cluster: P320 (≠ V298), S473 (= S451), C474 (= C452), G475 (= G453), K476 (= K454), C477 (= C455), C480 (= C458), L519 (= L497), C520 (= C498), L522 (≠ M500), G523 (= G501)
binding zinc ion: C457 (≠ D435), H544 (= H523)

Sites not aligning to the query:

binding iron/sulfur cluster: 560, 567, 570, 571, 573, 577, 590, 592, 597, 599, 600, 601, 603, 607
binding zinc ion: 549, 554

7t30B Structure of electron bifurcating ni-fe hydrogenase complex hydabcsl in fmn/NAD(h) bound state (see paper)
44% identity, 77% coverage: 112:523/533 of query aligns to 2:413/425 of 7t30B

query
sites
7t30B

N

K

Q

Q

Q

H

R

R

L

I

T

V

F

L

A

S

R

N

A

C

G

G

I

Y

T

I

D

D

P

P

V

E

S

K

I

I

A

E

D

E

Y

Y

L

I

A

A

H

R

D

D

G

G

F

Y

K

M

G

A

L

L

E

G

N

K

S

A

L

L

-

L

A

E

L

M

N

T

S

P

Q

E

A

E

I

V

V

L

D

E

E

E

V

V

K

K

A

K

S

S

G

G

L

L

R

R

G
|
G

R

R

G
|
G

A
|
A

A

G

F
|
F

P

P

T

T

G

G

I

L

K
|
K

W

W

Q

E

T
x
F

V

A

H

K

D

K

C

A

S

S

A

G

D

D

Q

K

K

K

Y

Y

I

V

V

I

C

C

N
|
N

A

A

D
|
D

E
|
E

G

G

D

D

S

P

G

G

T

A

F

F

A

M

D

D

R

R

L

S

V

T

M

L

E

E

A

G

D

D

P

P

L

H

M

S

L

V

V

I

E

E

G

G

M

M

I

T

I

I

A

G

G

A

V

Y

A

V

V

I

D

G

A

A

T

D

Q

E

G

G

Y

Y

I

I

Y

Y

L

C

R

R

S

A

E

E

Y

Y

P

P

V

L

A

A

Y

I

Q

K

I

R

M

L

N

K

E

I

A

A

I

I

A

A

N

Q

A

A

Y

E

A

E

A

M

G

G

Y

L

L

L

G

G

E

D

H

H

I

I

L

M

G

G

S

T

D

N

K

F

T

S

F

F

H

H

L

L

E

H

V

L

R

K

L

E

G

G

A

A

A

G

A

A

Y

F

I

V

C

C

G
|
G

E

E

E
|
E

T

T

S

A

L

L

L

M

E

A

S

S

L

I

E

E

G

G

K

R

R

R

G

G

M

M

V

P

R

R

F

P

K
x
R

P

P

L
x
F

P

P

A

A

I

Q

E

H

G

G

L

L

F

W

G

G

K

K

P

P

T

T

V

N

V

I

N

N

N
|
N

V

V

L

E

S

T

L

W

A

A

A

N

I

V

P

P

F

R

I

I

M

I

E

L

K

N

G

G

S

A

Q

D

A

W

Y

F

A

A

D

S

Y

M

G

G

M

T

G

E

R

K

S

S

R

K

G

G

T

T

L

K

P

I

F

F

Q

A

L

L

T

T

G

G

N

K

L

I

K

T

Q

N

G

T

G

G

L

L

V

I

E

E

L

V

A

P

F

M

G

G

P

I

T

T

L

I

R

R

E

E

L

I

V

I

D

Y

N

E

F

L

G

G

T

I

E

L

T

N

G

G

L

K

P

E

M

F

R

K

A

A

I

V

Q

Q

V

I

G

G

P

P

L

S

G

G

A

G

Y

C

L

L

P

T

A

K

S

E

Q

H

W

L

D

D

T

L

P

P

L

I

D

D

Y

Y

E

E

A

S

F

L

A

T

A

A

G

G

A

A

V
x
I

L
x
M

G

G

H
x
S

G

G

V

L

V

V

F

M

D

D

E

E

S

D

V

T

D
x
C

M

M

T

V

E

D

Q

V

A

A

R

K

F

F

S

F

M

L

E

E

F

F

C

T

V

Q

A

R

E

E

S
|
S

C
|
C

G
|
G

K

K

C
|
C

T

V

P

P

C
|
C

R

R

I

E

G

G

S

T

T

K

R

Q

G

M

V

L

E

L

V

M

I

L

D

Q

K

K

I

I

R
x
C

S

N

G

G

N

E

N

G

V

T

A

M

A

D

N

D

L

L

A

S

L

K

L

L

D

E

L

L

C

A

D

H

T

M

M

V

I

K

D

E

G

T

S
|
S

L

L

C
|
C

A
x
G

M
x
L

G
|
G

S

Q

M

T

T

A

P

P

I

N

P

P

V

V

K

I

S

T

A

T

F

I

K

R

Y

Y

F

F

S

R

E

D

D

E

I

Y

A

-

L

V

A

A

H
|
H

binding fe2/s2 (inorganic) cluster: C326 (≠ D435), C364 (≠ R473), H413 (= H523)
binding flavin mononucleotide: G54 (= G163), G56 (= G165), K65 (= K174), N82 (= N191), D84 (= D193), E85 (= E194), G173 (= G282), E175 (= E284), N210 (= N319), G390 (≠ A499), L391 (≠ M500)
binding nicotinamide-adenine-dinucleotide: G56 (= G165), G57 (= G166), A58 (= A167), F60 (= F169), K65 (= K174), F68 (≠ T177), E85 (= E194), E175 (= E284), R192 (≠ K301), F195 (≠ L304), I312 (≠ V421), M313 (≠ L422), S315 (≠ H424)
binding iron/sulfur cluster: S342 (= S451), C343 (= C452), G344 (= G453), C346 (= C455), C349 (= C458), S387 (= S496), C389 (= C498), L391 (≠ M500), G392 (= G501)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 419, 421, 424

7t2rB Structure of electron bifurcating ni-fe hydrogenase complex hydabcsl in fmn-free apo state (see paper)
44% identity, 77% coverage: 112:523/533 of query aligns to 2:413/425 of 7t2rB

query
sites
7t2rB

N

K

Q

Q

Q

H

R

R

L

I

T

V

F

L

A

S

R

N

A

C

G

G

I

Y

T

I

D

D

P

P

V

E

S

K

I

I

A

E

D

E

Y

Y

L

I

A

A

H

R

D

D

G

G

F

Y

K

M

G

A

L

L

E

G

N

K

S

A

L

L

-

L

A

E

L

M

N

T

S

P

Q

E

A

E

I

V

V

L

D

E

E

E

V

V

K

K

A

K

S

S

G

G

L

L

R

R

G

G

R

R

G

G

A

A

A

G

F

F

P

P

T

T

G

G

I

L

K

K

W

W

Q

E

T

F

V

A

H

K

D

K

C

A

S

S

A

G

D

D

Q

K

K

K

Y

Y

I

V

V

I

C

C

N

N

A

A

D

D

E

E

G

G

D

D

S

P

G

G

T

A

F

F

A

M

D

D

R

R

L

S

V

T

M

L

E

E

A

G

D

D

P

P

L

H

M

S

L

V

V

I

E

E

G

G

M

M

I

T

I

I

A

G

G

A

V

Y

A

V

V

I

D

G

A

A

T

D

Q

E

G

G

Y

Y

I

I

Y

Y

L

C

R

R

S

A

E

E

Y

Y

P

P

V

L

A

A

Y

I

Q

K

I

R

M

L

N

K

E

I

A

A

I

I

A

A

N

Q

A

A

Y

E

A

E

A

M

G

G

Y

L

L

L

G

G

E

D

H

H

I

I

L

M

G

G

S

T

D

N

K

F

T

S

F

F

H

H

L

L

E

H

V

L

R

K

L

E

G

G

A

A

A

G

A

A

Y

F

I

V

C

C

G

G

E

E

T

T

S

A

L

L

L

M

E

A

S

S

L

I

E

E

G

G

K

R

R

R

G

G

M

M

V
x
P

R

R

F

P

K

R

P

P

L

F

P

P

A

A

I

Q

E

H

G

G

L

L

F

W

G

G

K

K

P

P

T

T

V

N

V

I

N

N

V

V

L

E

S

T

L

W

A

A

A

N

I

V

P

P

F

R

I

I

M

I

E

L

K

N

G

G

S

A

Q

D

A

W

Y

F

A

A

D

S

Y

M

G

G

M

T

G

E

R

K

S

S

R

K

G

G

T

T

L

K

P

I

F

F

Q

A

L

L

T

T

G

G

N

K

L

I

K

T

Q

N

G

T

G

G

L

L

V

I

E

E

L

V

A

P

F

M

G

G

P

I

T

T

L

I

R

R

E

E

L

I

V

I

D

Y

N

E

F

L

G

G

T

I

E

L

T

N

G

G

L

K

P

E

M

F

R

K

A

A

I

V

Q

Q

V

I

G

G

P

P

L

S

G

G

A

G

Y

C

L

L

P

T

A

K

S

E

Q

H

W

L

D

D

T

L

P

P

L

I

D

D

Y

Y

E

E

A

S

F

L

A

T

A

A

G

G

A

A

V

I

L

M

G

G

H

S

G

G

V

L

V

V

F

M

D

D

E

E

S

D

V

T

D
x
C

M

M

T

V

E
x
D

Q

V

A

A

R

K

F

F

S

F

M

L

E

E

F

F

C

T

V

Q

A

R

E

E

S

S

C
|
C

G
|
G

K

K

C
|
C

T

V

P

P

C
|
C

R

R

I

E

G

G

S

T

T

K

R

Q

G

M

V

L

E

L

V

M

I

L

D

Q

K

K

I

I

R
x
C

S

N

G

G

N

E

N

G

V

T

A

M

A

D

N

D

L

L

A

S

L

K

L

L

D

E

L

L

C

A

D

H

T

M

M

V

I

K

D

E

G

T

S

S

L

L

C
|
C

A

G

M

L

G
|
G

S

Q

M

T

T

A

P

P

I

N

P

P

V

V

K

I

S

T

A

T

F

I

K

R

Y

Y

F

F

S

R

E

D

D

E

I

Y

A

-

L

V

A

A

H
|
H

binding fe2/s2 (inorganic) cluster: C326 (≠ D435), D329 (≠ E438), C364 (≠ R473), H413 (= H523)
binding iron/sulfur cluster: P189 (≠ V298), C343 (= C452), G344 (= G453), C346 (= C455), C349 (= C458), C389 (= C498), G392 (= G501)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 419, 421, 424

7q4vF Electron bifurcating hydrogenase - hydabc from a. Woodii (see paper)
47% identity, 73% coverage: 128:518/533 of query aligns to 1:394/470 of 7q4vF

query
sites
7q4vF

S

N

I

I

A

D

D

E

Y

Y

L

I

A

G

H

F

D

D

G

G

F

Y

K

L

G

A

L

L

E

E

N

K

S

V

L

L

-

L

A

T

L

M

N

S

S

P

Q

V

A

D

I

V

V

I

D

N

E

E

V

V

K

K

A

A

S

S

G

G

L

L

R

R

G
|
G

R

R

G
|
G

A

G

F
|
F

P

P

T

T

G

G

I

L

K
|
K

W

W

Q

Q

T

F

V

A

H

H

D

D

C

A

S

V

A

S

D

E

Q

D

-

G

-

I

K

K

Y

Y

I

V

V

A

C

C

N
|
N

A

A

D

D

E

E

G

G

D

D

S

P

G

G

T

A

F

F

A

M

D

D

R

R

L

S

V

V

M

L

E

E

A

G

D

D

P

P

L

H

M

A

L

V

V

I

E

E

G

A

M

M

I

A

I

I

A

A

G

G

V

Y

A

A

V

V

D

G

A

A

T

S

Q

K

G

G

Y

Y

I

V

Y

Y

L

V

R

R

S

A

E

E

Y

Y

P

P

V

I

A

A

Y

V

Q

N

I

R

M

L

N

Q

E

I

A

A

I

I

A

D

N

Q

A

A

Y

K

A

E

A

Y

G

G

Y

I

L

L

G

G

E

E

H

N

I

I

L

F

G

E

S

T

D

D

K

F

T

S

F

F

H

D

L

L

E

E

V

I

R

R

L

L

G

G

A

A

A

G

A

A

Y

F

I

V

C

C

G
|
G

E
|
E

T

T

S

A

L

L

L

M

E

N

S

S

L

I

E

E

G

G

K

K

R

R

G

G

M

E

V

P

R

R

F

P

K
x
R

P

P

L
x
F

P

P

A

A

I

N

E

K

G

G

L

L

F

F

G

G

K

K

P

P

T

T

V

V

V

L

N

N

V

V

L

E

S

T

L

Y

A

A

A

N

I

I

P

P

F

K

I

I

M

I

E

L

K

N

G

G

S

A

Q

E

A

W

Y

F

A

A

D

S

Y

V

G

G

M

T

G

E

R

K

S

S

R

K

G

G

T

T

L

K

P

V

F

F

Q

A

L

L

T

G

G

G

N

K

L

I

K

N

Q

N

G

T

G

G

L

L

V

L

E

E

L

I

A

P

F

M

G

G

P

T

T

T

L

L

R

R

E

E

L

I

V

I

D

Y

N

E

F

I

G

G

T

I

E

P

T

N

G

G

L

K

P

A

M

F

R

K

A

A

I

A

Q

Q

V

T

G

G

P

P

L

S

G

G

A

G

Y

C

L

L

P

P

A

E

S

S

Q

L

W

L

D

D

T

T

P

E

L

I

D

D

Y

Y

E

D

A

N

F

L

A

I

A

A

G

G

A

S

V
x
M

L

M

G

G

H

S

G

G

V

L

V

I

V

V

F

M

D

D

E

E

S

D

V

N

D
x
C

M

M

T

V

E

D

Q

V

A

A

R

R

F

F

S

F

M

L

E

D

F

F

C

T

V

Q

A

D

E

E

S
|
S

C
|
C

G
|
G

K
|
K

C
|
C

T

P

P

P

C
|
C

R

R

I

I

G

G

S

T

T

K

R

R

G

M

V

L

E

E

V

I

I

L

D

E

K

R

I

I

R

C

S

D

G

G

N

K

N

G

V

V

A

E

A

G

N

D

L

I

A

E

L

R

L

L

D

E

L

L

C

A

D

V

T

G

M

I

I

K

D

S

G

S

S

A

L
|
L

C
|
C

A
x
G

M

L

G

G

S

Q

M

T

T

A

P

P

I

N

P

P

V

V

K

L

S

S

A

T

F

I

K

R

Y

F

F

F

S

R

E

D

D

E

binding flavin mononucleotide: G37 (= G163), G39 (= G165), N67 (= N191), G158 (= G282), E159 (= E283), E160 (= E284), G375 (≠ A499)
binding nicotinamide-adenine-dinucleotide: G40 (= G166), F43 (= F169), K48 (= K174), R177 (≠ K301), F180 (≠ L304), M297 (≠ V421)
binding iron/sulfur cluster: S327 (= S451), C328 (= C452), G329 (= G453), K330 (= K454), C331 (= C455), C334 (= C458), L373 (= L497), C374 (= C498)
binding zinc ion: C311 (≠ D435)

Sites not aligning to the query: