SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_054343403.1 NCBI__GCF_001305295.1:WP_054343403.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P81601 Alcohol dehydrogenase class-3 chain L; Alcohol dehydrogenase class-III chain L; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Gadus morhua (Atlantic cod) (see 2 papers)
66% identity, 99% coverage: 4:374/376 of query aligns to 5:375/375 of P81601

query
sites
P81601

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Sites not aligning to the query:

1 modified: N-acetylalanine

P81600 Alcohol dehydrogenase class-3 chain H; Alcohol dehydrogenase class-III chain H; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Gadus morhua (Atlantic cod) (see 2 papers)
66% identity, 99% coverage: 4:374/376 of query aligns to 5:375/375 of P81600

query
sites
P81600

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Sites not aligning to the query:

1 modified: N-acetylalanine

P80467 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Saara hardwickii (Indian spiny-tailed lizard) (Uromastyx hardwickii) (see paper)
66% identity, 98% coverage: 6:374/376 of query aligns to 5:373/373 of P80467

query
sites
P80467

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Sites not aligning to the query:

1 modified: N-acetylalanine

P80572 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see 2 papers)
65% identity, 98% coverage: 4:372/376 of query aligns to 5:374/378 of P80572

query
sites
P80572

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N

H

K

P

D

D

A

A

P

P

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C

C

G

G

V

V

T

P

T

T

G

G

M

L

G

G

A

A

V

V

M

W

N

N

T

T

A

A

K

K

V

V

E

E

E

P

G

G

A

S

T

I

V

V

A

A

I

I

F

F

G

G

I

L

G

G

G

T

I

V

G

G

L

L

S

A

A

V

I

A

I

E

G

G

A

A

T

K

M

S

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

I

D

D

I

I

N

D

E

S

S

N

K

K

F

Y

E

D

L

T

A

A

R

K

K

N

L

F

G

G

A

V

T

T

D

E

C

F

I

I

N

N

P

P

K

K

D

D

Y

H

D

E

K

K

P

P

I

I

Q

Q

D

Q

V

V

I

I

V

I

E

D

L

L

T

T

D

D

G

G

V

V

D

D

Y

Y

S

S

F

F

E

E

C

C

I

L

G

G

N

N

V

V

N

S

V

V

M

M

R

R

S

S

A

A

L

L

E

E

C

C

H

H

K

K

G

G

W

W

G

G

E

T

S

S

V

V

V

I

I

V

G

G

V

V

A

A

G

A

A

S

G

G

Q

Q

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

F

K

K

G

S

R

R

S

S

E

Q

L

V

P

P

D

W

F

L

V

V

E

E

R

K

Y

Y

M

L

K

K

G

K

E

E

F

I

K

K

L

V

D

D

D

E

F

Y

I

I

T

T

H

H

T

N

M

L

T

T

L

L

E

L

E

E

I

I

N

N

T

K

A

A

F

F

D

D

L

L

M

L

H

H

E

E

G

G

K

Q

S

C

I

L

R

R

S

C

V

V

I

L

Sites not aligning to the query:

1 modified: N-acetylalanine

8co4C Crystal structure of apo s-nitrosoglutathione reductase from arabidopsis thalina (see paper)
64% identity, 99% coverage: 4:376/376 of query aligns to 6:379/379 of 8co4C

query
sites
8co4C

K

Q

T

V

I

I

K

T

S

C

K

K

A

A

I

V

A

A

W

Y

G

E

P

P

N

N

Q

K

P

P

L

L

S

V

I

I

E

E

E

D

V

V

D

Q

V

V

M

A

P

P

Q

Q

K

A

G

G

E

E

V

V

R

R

V

I

R

K

I

I

V

L

A

Y

S

T

G

A

V

L

C
|
C

H

H

T

T

D

D

A

A

F

Y

T

T

L

W

S

S

G

G

D

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

A

C

I

I

L

L

G

G

H
|
H

E

E

G

A

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

E

V

V

A

Q

V

A

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y

Y

T

Q

P

A

E

E

C
|
C

G

R

E

E

C
|
C

K

K

F

F

C
|
C

K

K

S

S

G

G

K

K

T

T

N

N

L

L

C
|
C

Q

G

K

K

I

V

R

R

E

S

T

A

Q

T

G

G

K

V

G

G

-

I

L

M

M

M

P

N

D

D

G

R

T

K

T

S

R

R

F

F

H

S

K

V

D

N

G

G

Q

K

P

P

I

I

F

Y

H

H

Y

F

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

Q

Y

Y

T

T

V

V

L

V

P

H

E

D

I

V

S

S

L

V

A

A

K

K

V

I

N

D

K

P

D

T

A

A

P

P

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C
|
C

G

G

V

V

T

P

T

T

G

G

M

L

G

G

A

A

V

V

M

W

N

N

T

T

A

A

K

K

V

V

E

E

E

P

G

G

A

S

T

N

V

V

A

A

I

I

F

F

G

G

I

L

G

G

G

T

I

V

G

G

L

L

S

A

A

V

I

A

I

E

G

G

A

A

T

K

M

T

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

I

D

D

I

I

N

D

E

S

S

K

K

K

F

Y

E

E

L

T

A

A

R

K

K

K

L

F

G

G

A

V

T

N

D

E

C

F

I

V

N

N

P

P

K

K

D

D

Y

H

D

D

K

K

P

P

I

I

Q

Q

D

E

V

V

I

I

V

V

E

D

L

L

T

T

D

D

G

G

V

V

D

D

Y

Y

S

S

F

F

E

E

C

C

I

I

G

G

N

N

V

V

N

S

V

V

M

M

R

R

S

A

A

A

L

L

E

E

C

C

H

H

K

K

G

G

W

W

G

G

E

T

S

S

V

V

V

I

I

V

G

G

V

V

A

A

G

A

A

S

G

G

Q

Q

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

F

K

K

G

S

R

R

S

T

E

Q

L

V

P

P

D

W

F

L

V

V

E

E

R

K

Y

Y

M

M

K

N

G

K

E

E

F

I

K

K

L

V

D

D

D

E

F

Y

I

I

T

T

H

H

T

N

M

L

T

T

L

L

E

G

E

E

I

I

N

N

T

K

A

A

F

F

D

D

L

L

M

L

H

H

E

E

G

G

K

T

S

C

I

L

R

R

S

C

V

V

I

L

H

D

F

T

D

S

K

K

binding zinc ion: C47 (= C45), H69 (= H67), C99 (= C97), C102 (= C100), C105 (= C103), C113 (= C111), C177 (= C174)

Q96533 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; Nitrosoglutathione reductase; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.-; EC 1.1.1.284 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
64% identity, 99% coverage: 4:376/376 of query aligns to 6:379/379 of Q96533

query
sites
Q96533

K

Q

T

V

I

I

K

T

S

C

K

K

A

A

I

V

A

A

W

Y

G

E

P

P

N

N

Q

K

P

P

L

L

S

V

I

I

E

E

E

D

V

V

D

Q

V

V

M

A

P

P

Q

Q

K

A

G

G

E

E

V

V

R

R

V

I

R

K

I

I

V

L

A

Y

S

T

G

A

V

L

C
|
C

H
|
H

T

T

D

D

A

A

F

Y

T

T

L

W

S

S

G

G

D

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

A

C

I

I

L

L

G

G

H
|
H

E
|
E

G

A

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

E

V

V

A

Q

V

A

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y

Y

T

Q

P

A

E

E

C
|
C

G

R

E

E

C
|
C

K

K

F

F

C
|
C

K

K

S

S

G

G

K

K

T

T

N

N

L

L

C
|
C

Q

G

K

K

I

V

R

R

E

S

T

A

Q

T

G

G

K

V

G

G

-

I

L

M

M

M

P

N

D

D

G

R

T

K

T

S

R

R

F

F

H

S

K

V

D

N

G

G

Q

K

P

P

I

I

F

Y

H

H

Y

F

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

Q

Y

Y

T

T

V

V

L

V

P

H

E

D

I

V

S

S

L

V

A

A

K

K

V

I

N

D

K

P

D

T

A

A

P

P

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C
|
C

G

G

V

V

T

P

T

T

G

G

M

L

G

G

A

A

V

V

M

W

N

N

T

T

A

A

K

K

V

V

E

E

E

P

G

G

A

S

T

N

V

V

A

A

I

I

F

F

G
|
G

I
x
L

G
|
G

G
x
T

I
x
V

G
|
G

L

L

S

A

A

V

I

A

I

E

G

G

A

A

T

K

M

T

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

I

D
|
D

I

I

N

D

E

S

S

K

K
|
K

F

Y

E

E

L

T

A

A

R

K

K

K

L

F

G

G

A

V

T

N

D

E

C

F

I

V

N

N

P

P

K

K

D

D

Y

H

D

D

K

K

P

P

I

I

Q

Q

D

E

V

V

I

I

V

V

E

D

L

L

T

T

D

D

G

G

V

V

D

D

Y

Y

S

S

F

F

E

E

C

C

I
|
I

G

G

N

N

V

V

N

S

V

V

M

M

R

R

S

A

A

A

L

L

E

E

C

C

H

H

K

K

G

G

W

W

G

G

E

T

S

S

V

V

V

I

I
x
V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

Q

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

R

K

G

G

S
x
T

A
|
A

F
|
F

G

G

V

F

K

K

G

S

R

R

S

T

E

Q

L

V

P

P

D

W

F

L

V

V

E

E

R

K

Y

Y

M

M

K

N

G

K

E

E

F

I

K

K

L

V

D

D

D

E

F

Y

I

I

T

T

H

H

T

N

M

L

T

T

L

L

E

G

E

E

I

I

N

N

T

K

A

A

F

F

D

D

L

L

M

L

H

H

E

E

G

G

K

T

S

C

I

L

R
|
R

S

C

V

V

I

L

H

D

F

T

D

S

K

K

C47 (= C45) binding Zn(2+)
H48 (= H46) binding NAD(+)
H69 (= H67) binding Zn(2+)
E70 (= E68) binding Zn(2+)
C99 (= C97) binding Zn(2+)
C102 (= C100) binding Zn(2+)
C105 (= C103) binding Zn(2+)
C113 (= C111) binding Zn(2+)
C177 (= C174) binding Zn(2+)
GLGTVG 202:207 (≠ GIGGIG 199:204) binding NAD(+)
D226 (= D223) binding NAD(+)
K231 (= K228) binding NAD(+)
I272 (= I269) binding NAD(+)
VGV 295:297 (≠ IGV 292:294) binding NAD(+)
TAF 320:322 (≠ SAF 317:319) binding NAD(+)
R372 (= R369) binding NAD(+)

4gl4A Crystal structure of oxidized s-nitrosoglutathione reductase from arabidopsis thalina, complex with nadh
64% identity, 99% coverage: 4:376/376 of query aligns to 5:378/378 of 4gl4A

query
sites
4gl4A

K

Q

T

V

I

I

K

T

S

C

K

K

A

A

I

V

A

A

W

Y

G

E

P

P

N

N

Q

K

P

P

L

L

S

V

I

I

E

E

E

D

V

V

D

Q

V

V

M

A

P

P

Q

Q

K

A

G

G

E

E

V

V

R

R

V

I

R

K

I

I

V

L

A

Y

S

T

G

A

V

L

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

W

S

S

G

G

D

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

A

C

I

I

L

L

G

G

H
|
H

E
|
E

G

A

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

E

V

V

A

Q

V

A

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y
|
Y

T

Q

P

A

E

E

C
|
C

G

R

E

E

C
|
C

K

K

F

F

C
|
C

K

K

S

S

G

G

K

K

T

T

N

N

L

L

C
|
C

Q

G

K

K

I

V

R
|
R

E

S

T

A

Q

T

G

G

K

V

G

G

-

I

L

M

M

M

P

N

D

D

G

R

T

K

T

S

R

R

F

F

H

S

K

V

D

N

G

G

Q

K

P

P

I

I

F

Y

H

H

Y

F

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

Q

Y

Y

T

T

V

V

L

V

P

H

E

D

I

V

S

S

L

V

A

A

K

K

V

I

N

D

K

P

D

T

A

A

P

P

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C
|
C

G

G

V

V

T

P

T
|
T

G

G

M

L

G

G

A

A

V

V

M

W

N

N

T

T

A

A

K

K

V

V

E

E

E

P

G

G

A

S

T

N

V

V

A

A

I

I

F

F

G

G

I

L

G
|
G

G

T

I
x
V

G

G

L

L

S

A

A

V

I

A

I

E

G

G

A

A

T

K

M

T

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

I

D
|
D

I
|
I

N

D

E

S

S

K

K

K

F

Y

E

E

L

T

A

A

R

K

K

K

L

F

G

G

A

V

T

N

D

E

C

F

I

V

N

N

P

P

K

K

D

D

Y

H

D

D

K

K

P

P

I

I

Q

Q

D

E

V

V

I

I

V

V

E

D

L

L

T

T

D

D

G

G

V

V

D

D

Y

Y

S

S

F

F

E

E

C
|
C

I
|
I

G

G

N

N

V

V

N

S

V

V

M

M

R

R

S

A

A

A

L

L

E

E

C

C

H

H

K

K

G

G

W

W

G

G

E

T

S

S

V

V

V

I

I
x
V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

Q

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

R

K

G

G

S

T

A
|
A

F

F

G

G

V

F

K

K

G

S

R

R

S

T

E

Q

L

V

P

P

D

W

F

L

V

V

E

E

R

K

Y

Y

M

M

K

N

G

K

E

E

F

I

K

K

L

V

D

D

D

E

F

Y

I

I

T

T

H

H

T

N

M

L

T

T

L

L

E

G

E

E

I

I

N

N

T

K

A

A

F

F

D

D

L

L

M

L

H

H

E

E

G

G

K

T

S

C

I

L

R
|
R

S

C

V

V

I

L

H

D

F

T

D

S

K

K

active site: C46 (= C45), H47 (= H46), T48 (= T47), Y51 (≠ F50), H68 (= H67), E69 (= E68), C98 (= C97), C101 (= C100), C104 (= C103), C112 (= C111), R116 (= R115), C176 (= C174), T180 (= T178), R371 (= R369)
binding 1,4-dihydronicotinamide adenine dinucleotide: H47 (= H46), Y94 (= Y93), T180 (= T178), G203 (= G201), V205 (≠ I203), D225 (= D223), I226 (= I224), C270 (= C268), I271 (= I269), V294 (≠ I292), G295 (= G293), V296 (= V294), A320 (= A318)
binding zinc ion: C46 (= C45), H68 (= H67), E69 (= E68), C98 (= C97), C101 (= C100), C104 (= C103), C112 (= C111), C176 (= C174)

3ukoA Crystal structure of s-nitrosoglutathione reductase from arabidopsis thaliana, complex with nadh
64% identity, 99% coverage: 4:376/376 of query aligns to 5:378/378 of 3ukoA

query
sites
3ukoA

K

Q

T

V

I

I

K

T

S

C

K

K

A

A

I

V

A

A

W

Y

G

E

P

P

N

N

Q

K

P

P

L

L

S

V

I

I

E

E

E

D

V

V

D

Q

V

V

M

A

P

P

Q

Q

K

A

G

G

E

E

V

V

R

R

V

I

R

K

I

I

V

L

A

Y

S

T

G

A

V

L

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

W

S

S

G

G

D

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

A

C

I

I

L

L

G

G

H
|
H

E
|
E

G

A

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

E

V

V

A

Q

V

A

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y

Y

T

Q

P

A

E

E

C
|
C

G

R

E

E

C
|
C

K

K

F

F

C
|
C

K

K

S

S

G

G

K

K

T

T

N

N

L

L

C
|
C

Q

G

K

K

I

V

R
|
R

E

S

T

A

Q

T

G

G

K

V

G

G

-

I

L

M

M

M

P

N

D

D

G

R

T

K

T

S

R

R

F

F

H

S

K

V

D

N

G

G

Q

K

P

P

I

I

F

Y

H

H

Y

F

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

Q

Y

Y

T

T

V

V

L

V

P

H

E

D

I

V

S

S

L

V

A

A

K

K

V

I

N

D

K

P

D

T

A

A

P

P

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C
|
C

G

G

V

V

T

P

T
|
T

G

G

M

L

G

G

A

A

V

V

M

W

N

N

T

T

A

A

K

K

V

V

E

E

E

P

G

G

A

S

T

N

V

V

A

A

I

I

F

F

G

G

I

L

G
|
G

G

T

I
x
V

G

G

L

L

S

A

A

V

I

A

I

E

G

G

A

A

T

K

M

T

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

I

D
|
D

I
|
I

N

D

E

S

S

K

K
|
K

F

Y

E

E

L

T

A

A

R

K

K

K

L

F

G

G

A

V

T

N

D

E

C

F

I

V

N

N

P

P

K

K

D

D

Y

H

D

D

K

K

P

P

I

I

Q

Q

D

E

V

V

I

I

V

V

E

D

L

L

T

T

D

D

G

G

V

V

D

D

Y

Y

S

S

F

F

E

E

C
|
C

I
|
I

G

G

N

N

V

V

N

S

V

V

M

M

R

R

S

A

A

A

L

L

E

E

C

C

H

H

K

K

G

G

W

W

G

G

E

T

S

S

V

V

V

I

I
x
V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

Q

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

R

K

G

G

S

T

A
|
A

F

F

G

G

V

F

K

K

G

S

R

R

S

T

E

Q

L

V

P

P

D

W

F

L

V

V

E

E

R

K

Y

Y

M

M

K

N

G

K

E

E

F

I

K

K

L

V

D

D

D

E

F

Y

I

I

T

T

H

H

T

N

M

L

T

T

L

L

E

G

E

E

I

I

N

N

T

K

A

A

F

F

D

D

L

L

M

L

H

H

E

E

G

G

K

T

S

C

I

L

R
|
R

S

C

V

V

I

L

H

D

F

T

D

S

K

K

active site: C46 (= C45), H47 (= H46), T48 (= T47), Y51 (≠ F50), H68 (= H67), E69 (= E68), C98 (= C97), C101 (= C100), C104 (= C103), C112 (= C111), R116 (= R115), C176 (= C174), T180 (= T178), R371 (= R369)
binding nicotinamide-adenine-dinucleotide: H47 (= H46), T180 (= T178), G203 (= G201), V205 (≠ I203), D225 (= D223), I226 (= I224), K230 (= K228), C270 (= C268), I271 (= I269), V294 (≠ I292), G295 (= G293), V296 (= V294), A320 (= A318)
binding zinc ion: C46 (= C45), H68 (= H67), E69 (= E68), C98 (= C97), C101 (= C100), C104 (= C103), C112 (= C111), C176 (= C174)

4dl9A Crystal structure of s-nitrosoglutathione reductase from tomato (solanum lycopersicum) in complex with NAD+ (see paper)
64% identity, 98% coverage: 4:372/376 of query aligns to 6:375/379 of 4dl9A

query
sites
4dl9A

K

Q

T

V

I

I

K

T

S

C

K

K

A

A

I

V

A

A

W

W

G

E

P

P

N

N

Q

K

P

P

L

L

S

V

I

I

E

E

E

D

V

V

D

Q

V

V

M

A

P

P

Q

Q

K

A

G

G

E

E

V

V

R

R

V

V

R

K

I

V

V

L

A

Y

S

T

G

A

V

L

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

W

S

S

G

G

D

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

A

C

I

V

L

L

G

G

H
|
H

E
|
E

G

A

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

E

V

V

A

Q

V

P

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y
|
Y

T

Q

P

A

E

E

C
|
C

G

R

E

E

C
|
C

K

K

F

F

C
|
C

K

K

S

S

G

G

K

K

T

T

N

N

L

L

C
|
C

Q

G

K

K

I

V

R
|
R

E

A

T

A

Q

T

G

G

K

V

G

G

-

V

L

M

M

M

P

N

D

D

G

R

T

K

T

S

R

R

F

F

H

S

K

I

D

N

G

G

Q

K

P

P

I

I

F

Y

H

H

Y

F

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

Q

Y

Y

T

T

V

V

L

V

P

H

E

D

I

V

S

S

L

V

A

A

K

K

V

I

N

D

K

P

D

V

A

A

P

P

L

L

E

E

E

K

I

V

C

C

L

L

G

G

C
|
C

G

G

V

V

T

P

T
|
T

G

G

M

L

G

G

A

A

V

V

M

W

N

N

T

T

A

A

K

K

V

V

E

E

E

P

G

G

A

S

T

I

V

V

A

A

I

V

F

F

G

G

I

L

G
|
G

G

T

I
x
V

G

G

L

L

S

A

A

V

I

A

I

E

G

G

A

A

T

K

M

A

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

I

D
|
D

I
|
I

N

D

E

S

S

K

K
|
K

F

F

E

D

L

R

A

A

R

K

K

N

L

F

G

G

A

V

T

T

D

E

C

F

I

I

N

N

P

P

K

K

D

E

Y

H

D

E

K

Q

P

P

I

I

Q

Q

D

Q

V

V

I

I

V

V

E

D

L

L

T

T

D

D

G

G

V

V

D

D

Y

Y

S

S

F

F

E

E

C
|
C

I
|
I

G

G

N

N

V

V

N

S

V

V

M

M

R

R

S

S

A

A

L

L

E

E

C

C

H

H

K

K

G

G

W

W

G

G

E

T

S

S

V

V

V

I

I
x
V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

Q

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

R

K

G

G

S

T

A
|
A

F
|
F

G

G

V

F

K

K

G

S

R

R

S

S

E

Q

L

V

P

P

D

S

F

L

V

V

E

D

R

K

Y

Y

M

L

K

K

G

K

E

E

F

I

K

K

L

V

D

D

D

E

F

Y

I

I

T

T

H

H

T

N

M

M

T

T

L

L

E

A

E

D

I

I

N

N

T

K

A

A

F

F

D

D

L

L

M

M

H

H

E

D

G

G

K

D

S

C

I

L

R
|
R

S

V

V

V

I

L

active site: C47 (= C45), H48 (= H46), T49 (= T47), Y52 (≠ F50), H69 (= H67), E70 (= E68), C99 (= C97), C102 (= C100), C105 (= C103), C113 (= C111), R117 (= R115), C177 (= C174), T181 (= T178), R372 (= R369)
binding nicotinamide-adenine-dinucleotide: Y95 (= Y93), T181 (= T178), G204 (= G201), V206 (≠ I203), D226 (= D223), I227 (= I224), K231 (= K228), C271 (= C268), I272 (= I269), V295 (≠ I292), G296 (= G293), V297 (= V294), A321 (= A318), F322 (= F319)
binding zinc ion: C47 (= C45), H69 (= H67), E70 (= E68), C99 (= C97), C102 (= C100), C105 (= C103), C113 (= C111), C177 (= C174)

P11766 Alcohol dehydrogenase class-3; Alcohol dehydrogenase 5; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Homo sapiens (Human) (see 3 papers)
65% identity, 99% coverage: 1:372/376 of query aligns to 1:372/374 of P11766

query
sites
P11766

M
|
M

N

A

N

N

K

E

T

V

I

I

K

K

S

C

K

K

A

A

I

V

A

A

W

W

G

E

P

A

N

G

Q

K

P

P

L

L

S

S

I

I

E

E

V

I

D

E

V

V

M

A

P

P

Q

K

K

A

G

H

E

E

V

V

R

R

V

I

R

K

I

I

V

I

A

A

S

T

G

A

V

V

C
|
C

H

H

T

T

D

D

A

A

F

Y

T

T

L

L

S

S

G

G

D

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

I

I

L

L

G

G

H
|
H

E

E

G

G

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A

K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

I

P

P

E

Q

C
|
C

G

G

E

E

C
|
C

K

K

F

F

C
|
C

K

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q

Q

K

K

I

I

R
|
R

E

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

T

S

R

R

F

F

H

T

K

C

D

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

L

V

A

A

K

K

V

I

N

D

K

P

D
x
L

A

A

P

P

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T

T

G

G

M

Y

G

G

A

A

V

A

M

V

N

N

T

T

A

A

K

K

V

L

E

E

E

P

G

G

A

S

T

V

V

C

A

A

I

V

F

F

G

G

I

L

G

G

I

V

G

G

L

L

S

A

A

V

I

I

I

M

G

G

A

C

T

K

M

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

V

D

D

I

I

N

N

E

K

S

D

K

K

F

F

E

A

L

R

A

A

R

K

K

E

L

F

G

G

A

A

T

T

D

E

C

C

I

I

N

N

P

P

K

Q

D

D

Y

F

D

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

L

M

T

T

D

D

G

G

V

V

D

D

Y

Y

S

S

F

F

E

E

C

C

I

I

G

G

N

N

V

V

N

K

V

V

M

M

R

R

S

A

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

G

G

V

V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V
|
V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

W

K

K

G

S

R

V

S

E

E

S

L

V

P

P

D

K

F

L

V

V

E

S

R

E

Y

Y

M

M

K

S

G

K

E

K

F

I

K

K

L

V

D

D

D

E

F

F

I

V

T

T

H

H

T

N

M

L

T

S

L

F

E

D

E

E

I

I

N

N

T

K

A

A

F

F

D

E

L

L

M

M

H

H

E

S

G

G

K

K

S

S

I

I

R

R

S

T

V

V

I

V

M1 (= M1) modified: Initiator methionine, Removed
C45 (= C45) binding Zn(2+)
H67 (= H67) binding Zn(2+)
C97 (= C97) binding Zn(2+)
C100 (= C100) binding Zn(2+)
C103 (= C103) binding Zn(2+)
C111 (= C111) binding Zn(2+)
R115 (= R115) Important for FDH activity and activation by fatty acids; mutation R->A,D: Loss of FDH activity and loss of activation by fatty acids.
L163 (≠ D163) to S: in dbSNP:rs28730623
C174 (= C174) binding Zn(2+)
V309 (= V309) to I: in dbSNP:rs28730628

P19854 Alcohol dehydrogenase class-3; Alcohol dehydrogenase 5; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Equus caballus (Horse) (see 2 papers)
64% identity, 99% coverage: 1:374/376 of query aligns to 1:374/374 of P19854

query
sites
P19854

M
|
M

N
x
S

N

A

K

E

T

V

I

I

K

K

S

C

K

K

A

A

I

V

A

A

W

W

G

E

P

A

N

G

Q

K

P

P

L

V

S

S

I

I

E

E

V

V

D

E

V

V

M

A

P

P

Q

K

K

A

G

H

E

E

V

V

R

R

V

I

R

K

I

I

V

I

A

A

S

T

G

A

V

V

C

C

H

H

T

T

D

D

A

A

F

Y

T

T

L

L

S

S

G

G

D

A

D

D

P

P

E

E

G

G

I

S

F

F

P

P

A

V

I

I

L

L

G

G

H

H

E

E

G

G

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A

K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

I

P

P

E

Q

C

C

G

G

E

E

C

C

K

K

F

F

C

C

K

L

S

N

G

P

K

Q

T

T

N

N

L

L

C

C

Q

Q

K

K

I

I

R

R

E

T

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

T

S

R

R

F

F

H

T

K

C

D

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

L

V

A

A

K

K

V

I

N

D

K

P

D

L

A

A

P

P

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C

C

G

G

V

V

T

S

T

T

G

G

M

Y

G

G

A

A

V

A

M

V

N

N

T

T

A

A

K

K

V

V

E

E

E

P

G

G

A

S

T

T

V

C

A

A

I

I

F

F

G

G

I

L

G

G

I

V

G

G

L

L

S

A

A

V

I

I

I

M

G

G

A

C

T

K

M

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

V

D

D

I

I

N

N

E

K

S

D

K

K

F

F

E

A

L

K

A

A

R

K

K

E

L

F

G

G

A

A

T

S

D

E

C

C

I

I

N

N

P

P

K

Q

D

D

Y

F

D

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

L

M

T

T

D

D

G

G

V

V

D

D

Y

Y

S

S

F

F

E

E

C

C

I

I

G

G

N

N

V

V

N

K

V

V

M

M

R

R

S

A

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

G

G

V

V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

W

K

K

G

S

R

V

S

E

E

S

L

I

P

P

D

K

F

L

V

V

E

S

R

E

Y

Y

M

M

K

S

G

K

E

K

F

I

K

K

L

V

D

D

D

E

F

F

I

V

T

T

H

H

T

S

M

L

T

S

L

F

E

D

E

Q

I

I

N

N

T

E

A

A

F

F

D

E

L

L

M

M

H

H

E

A

G

G

K

K

S

S

I

I

R

R

S

T

V

V

I

V

H

K

F

L

M1 (= M1) modified: Initiator methionine, Removed
S2 (≠ N2) modified: N-acetylserine

8gv3A The cryo-em structure of gsnor with nyy001
64% identity, 99% coverage: 3:375/376 of query aligns to 1:373/373 of 8gv3A

query
sites
8gv3A

N

N

K

E

T

V

I

I

K

K

S

C

K

K

A

A

I

V

A

A

W

W

G

E

P

A

N

G

Q

K

P

P

L

L

S

S

I

I

E

E

V

I

D

E

V

V

M

A

P

P

Q

K

K

A

G

H

E

E

V

V

R

R

V

I

R

K

I

I

V

I

A

A

S

T

G

A

V

V

C
|
C

H

H

T
|
T

D

D

A

A

F

Y

T

T

L

L

S

S

G

G

D

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

I

I

L

L

G

G

H
|
H

E

E

G

G

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A

K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T
x
I

P
|
P

E

Q

C

C

G

G

E

E

C

C

K

K

F

F

C
|
C

K

L

S

N

G

P

K

K

T

T

N

N

L
|
L

C
|
C

Q

Q

K

K

I

I

R

R

E

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

T

S

R

R

F

F

H

T

K

C

D

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M
|
M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

L

V

A

A

K

K

V

I

N

D

K

P

D

L

A

A

P

P

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T

T

G

G

M

Y

G

G

A

A

V

A

M

V

N

N

T

T

A

A

K

K

V

L

E

E

E

P

G

G

A

S

T

V

V

C

A

A

I

V

F

F

G

G

I

L

G

G

I
x
V

G

G

L

L

S

A

A

V

I

I

I

M

G

G

A

C

T

K

M

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

V

D
|
D

I
|
I

N

N

E

K

S

D

K

K

F

F

E

A

L

R

A

A

R

K

K

E

L

F

G

G

A

A

T

T

D

E

C

C

I

I

N

N

P

P

K

Q

D

D

Y

F

D

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

L

M

T

T

D

D

G

G

V

V

D

D

Y

Y

S

S

F

F

E

E

C
|
C

I
|
I

G

G

N

N

V

V

N

K

V

V

M

M

R

R

S

A

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I
x
V

G
|
G

V

V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

W

K

K

G

S

R

V

S

E

E

S

L

V

P

P

D

K

F

L

V

V

E

S

R

E

Y

Y

M

M

K

S

G

K

E

K

F

I

K

K

L

V

D

D

D

E

F

F

I

V

T

T

H

H

T

N

M

L

T

S

L

F

E

D

E

E

I

I

N

N

T

K

A

A

F

F

D

E

L

L

M

M

H

H

E

S

G

G

K

K

S

S

I

I

R

R

S

T

V

V

I

V

H

K

F

I

D

E

binding nicotinamide-adenine-dinucleotide: C172 (= C174), V201 (≠ I203), D221 (= D223), I222 (= I224), C266 (= C268), I267 (= I269), V290 (≠ I292), G291 (= G293)
binding (4P)-4-{2-[4-(1H-imidazol-1-yl)phenyl]-5-[3-oxo-3-(2-oxo-1,3-thiazolidin-3-yl)propyl]-1H-pyrrol-1-yl}-3-methylbenzamide: T45 (= T47), Y91 (= Y93), I92 (≠ T94), P93 (= P95), L108 (= L110), C109 (= C111), M139 (= M141), C172 (= C174)
binding zinc ion: C43 (= C45), H65 (= H67), C101 (= C103), C109 (= C111), C172 (= C174)

P81431 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Octopus vulgaris (Common octopus) (see paper)
64% identity, 98% coverage: 4:372/376 of query aligns to 6:374/378 of P81431

query
sites
P81431

K

K

T

P

I

I

K

K

S

C

K

M

A

A

I

I

A

A

W

W

G

E

P

A

N

K

Q

K

P

P

L

L

S

S

I

I

E

E

V

V

D

E

V

V

M

A

P

P

Q

K

K

S

G

G

E

E

V

V

R

R

V

I

R

K

I

I

V

L

A

H

S

S

G

G

V

V

C

C

H

H

T

T

D

D

A

A

F

Y

T

T

L

L

S

E

G

G

D

I

D

D

P

P

E

E

G

G

I

I

F

F

P

P

A

V

I

I

L

L

G

G

H

H

E

E

G

G

A

A

G

G

I

I

V

V

E

E

S

S

I

I

G

G

E

E

G

G

V

V

T

T

S

S

V

V

A

K

V

P

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

V

P

P

E

Q

C

C

G

Y

E

E

C

C

K

K

F

F

C

C

K

L

S

N

G

P

K

K

T

T

N

N

L

L

C

C

Q

Q

K

K

I

I

R

R

E

V

T

T

Q

Q

G

G

K

K

G

G

L

V

M

M

P

P

D

D

G

G

T

T

R

R

F

F

H

T

K

C

D

K

G

G

Q

K

P

E

I

V

F

F

H

H

Y

F

M

M

G

G

C

C

S

S

T

T

F

F

S

S

E

E

Y

F

T

T

V

V

L

V

P

A

E

E

I

I

S

S

L

I

A

A

K

K

V

V

N

N

K

P

D

K

A

A

P

A

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C

C

G

G

V

I

T

S

T

T

G

G

M

Y

G

G

A

A

V

A

M

L

N

N

T

T

A

A

K

A

V

V

E

E

E

K

G

G

A

S

T

N

V

C

A

A

I

V

F

W

G

G

I

L

G

G

G

A

I

V

G

G

L

L

S

A

A

V

I

A

I

M

G

G

A

C

T

K

M

A

A

A

K

G

A

A

S

A

R

R

I

I

A

G

I

I

D

D

I

I

N

N

E

E

S

S

K

K

F

F

E

E

L

I

A

G

R

K

K

K

L

F

G

G

A

I

T

T

D

E

C

F

I

V

N

N

P

P

K

K

D

K

Y

F

D

C

K

K

P

S

I

V

Q

Q

D

K

V

V

I

L

V

G

E

E

L

M

T

T

D

D

G

V

G

G

V

V

D

D

Y

F

S

T

F

F

E

E

C

C

I

I

G

G

N

N

V

V

N

G

V

V

M

M

R

R

S

A

A

A

L

L

E

E

C

S

C

C

H

H

K

K

G

G

W

W

G

G

E

T

S

S

V

V

V

I

I

V

G

G

V

V

A

A

G

A

A

H

G

G

Q

E

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

F

K

K

G

S

R

R

S

D

E

S

L

V

P

P

D

K

F

L

V

V

E

D

R

D

Y

Y

M

M

K

N

G

K

E

K

F

L

K

L

L

L

D

D

D

E

F

F

I

V

T

S

H

H

T

K

M

L

T

S

L

F

E

K

I

I

N

N

T

E

A

A

F

F

D

D

L

L

M

M

H

H

E

K

G

G

K

E

S

S

I

I

R

R

S

A

V

V

I

L

Sites not aligning to the query:

1 modified: N-acetylthreonine

3qj5A S-nitrosoglutathione reductase (gsnor) in complex with n6022 (see paper)
65% identity, 98% coverage: 3:372/376 of query aligns to 1:370/372 of 3qj5A

query
sites
3qj5A

N

N

K

E

T

V

I

I

K

K

S

C

K

K

A

A

I

V

A

A

W

W

G

E

P

A

N

G

Q

K

P

P

L

L

S

S

I

I

E

E

V

I

D

E

V

V

M

A

P

P

Q

K

K

A

G

H

E

E

V

V

R

R

V

I

R

K

I

I

V

I

A

A

S

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

L

S

S

G

G

D

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

I

I

L

L

G

G

H
|
H

E
|
E

G

G

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A

K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T
x
I

P

P

E

Q

C
|
C

G

G

E

E

C
|
C

K

K

F

F

C
|
C

K

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q
|
Q

K

K

I

I

R
|
R

E

V

T

T

Q
|
Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

T

S

R

R

F

F

H

T

K

C

D

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

L

V

A

A

K

K

V

I

N

D

K

P

D

L

A

A

P

P

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

M

Y

G

G

A

A

V

A

M

V

N

N

T

T

A

A

K

K

V

L

E

E

E

P

G

G

A

S

T

V

V

C

A

A

I

V

F

F

G

G

I

L

G
|
G

G

G

I
x
V

G

G

L

L

S

A

A

V

I

I

I

M

G

G

A

C

T

K

M

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

V

D
|
D

I
|
I

N

N

E

K

S

D

K

K

F

F

E

A

L

R

A

A

R

K

K

E

L

F

G

G

A

A

T

T

D

E

C

C

I

I

N

N

P

P

K

Q

D

D

Y

F

D

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

L

M

T

T

D

D

G

G

V

V

D

D

Y

Y

S

S

F

F

E

E

C
|
C

I
|
I

G

G

N

N

V

V

N

K

V

V

M

M

R

R

S

A

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I
x
V

G
|
G

V
|
V

A

A

G
x
A

A

S

G

G

Q

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A
|
A

F
|
F

G

G

V

W

K

K

G

S

R

V

S

E

E

S

L

V

P

P

D

K

F

L

V

V

E

S

R

E

Y

Y

M

M

K

S

G

K

E

K

F

I

K

K

L

V

D

D

D

E

F

F

I

V

T

T

H

H

T

N

M

L

T

S

L

F

E

D

E

E

I

I

N

N

T

K

A

A

F

F

D

E

L

L

M

M

H

H

E

S

G

G

K

K

S

S

I

I

R
|
R

S

T

V

V

I

V

active site: C43 (= C45), H44 (= H46), T45 (= T47), Y48 (≠ F50), H65 (= H67), E66 (= E68), C95 (= C97), C98 (= C100), C101 (= C103), C109 (= C111), R113 (= R115), C172 (= C174), T176 (= T178), R367 (= R369)
binding 3-{1-(4-carbamoyl-2-methylphenyl)-5-[4-(1H-imidazol-1-yl)phenyl]-1H-pyrrol-2-yl}propanoic acid: C43 (= C45), T45 (= T47), H65 (= H67), Y91 (= Y93), I92 (≠ T94), Q110 (= Q112), Q116 (= Q118), C172 (= C174)
binding pentane-1,5-diamine: H44 (= H46), Y48 (≠ F50), V292 (= V294), A294 (≠ G296)
binding nicotinamide-adenine-dinucleotide: C172 (= C174), T176 (= T178), G199 (= G201), V201 (≠ I203), D221 (= D223), I222 (= I224), C266 (= C268), I267 (= I269), V290 (≠ I292), G291 (= G293), V292 (= V294), A316 (= A318), F317 (= F319)
binding zinc ion: C43 (= C45), H65 (= H67), C95 (= C97), C98 (= C100), C101 (= C103), C109 (= C111), C172 (= C174)

2fzeA Crystal structure of the binary complex of human glutathione-dependent formaldehyde dehydrogenase with adp-ribose (see paper)
65% identity, 98% coverage: 3:372/376 of query aligns to 2:371/373 of 2fzeA

query
sites
2fzeA

N

N

K

E

T

V

I

I

K

K

S

C

K

K

A

A

I

V

A

A

W

W

G

E

P

A

N

G

Q

K

P

P

L

L

S

S

I

I

E

E

V

I

D

E

V

V

M

A

P

P

Q

K

K

A

G

H

E

E

V

V

R

R

V

I

R

K

I

I

V

I

A

A

S

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

L

S

S

G

G

D

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

I

I

L

L

G

G

H
|
H

E
|
E

G

G

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A
x
K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

I

P

P

E

Q

C
|
C

G

G

E

E

C
|
C

K

K

F

F

C
|
C

K

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q

Q

K

K

I

I

R
|
R

E

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

T

S

R

R

F

F

H

T

K

C

D

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

L

V

A

A

K
|
K

V

I

N

D

K

P

D

L

A

A

P

P

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

M

Y

G

G

A

A

V

A

M

V

N

N

T

T

A

A

K

K

V

L

E

E

E

P

G

G

A

S

T

V

V

C

A

A

I

V

F

F

G

G

I

L

G
|
G

G

G

I
x
V

G

G

L

L

S

A

A

V

I

I

I

M

G

G

A

C

T

K

M

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

V

D
|
D

I
|
I

N

N

E

K

S

D

K

K

F

F

E

A

L

R

A

A

R

K

K

E

L

F

G

G

A

A

T

T

D

E

C

C

I

I

N

N

P

P

K

Q

D

D

Y

F

D

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

L

M

T

T

D

D

G

G

V

V

D

D

Y

Y

S

S

F

F

E

E

C

C

I
|
I

G

G

N

N

V

V

N

K

V

V

M

M

R

R

S

A

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

G
|
G

V

V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

W

K

K

G

S

R

V

S

E

E

S

L

V

P

P

D

K

F

L

V

V

E

S

R

E

Y

Y

M

M

K

S

G

K

E

K

F

I

K

K

L

V

D

D

D

E

F

F

I

V

T

T

H

H

T

N

M

L

T

S

L

F

E

D

E

E

I

I

N

N

T

K

A

A

F

F

D

E

L

L

M

M

H

H

E

S

G

G

K

K

S

S

I

I

R
|
R

S

T

V

V

I

V

active site: C44 (= C45), H45 (= H46), T46 (= T47), Y49 (≠ F50), H66 (= H67), E67 (= E68), C96 (= C97), C99 (= C100), C102 (= C103), C110 (= C111), R114 (= R115), C173 (= C174), T177 (= T178), R368 (= R369)
binding adenosine-5-diphosphoribose: G200 (= G201), V202 (≠ I203), D222 (= D223), I223 (= I224), I268 (= I269), G292 (= G293)
binding phosphate ion: K83 (≠ A84), K158 (= K159)
binding zinc ion: H66 (= H67), C96 (= C97), C99 (= C100), C102 (= C103), C110 (= C111), C173 (= C174)

1mc5A Ternary complex of human glutathione-dependent formaldehyde dehydrogenase with s-(hydroxymethyl)glutathione and nadh (see paper)
65% identity, 98% coverage: 3:372/376 of query aligns to 2:371/373 of 1mc5A

query
sites
1mc5A

N

N

K

E

T

V

I

I

K

K

S

C

K

K

A

A

I

V

A

A

W

W

G

E

P

A

N

G

Q

K

P

P

L

L

S

S

I

I

E

E

V

I

D

E

V

V

M

A

P

P

Q

K

K

A

G

H

E

E

V

V

R

R

V

I

R

K

I

I

V

I

A

A

S

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

L

S

S

G

G

D

A

D

D

P
|
P

E
|
E

G

G

I

C

F

F

P

P

A

V

I

I

L

L

G

G

H
|
H

E
|
E

G

G

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A

K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T

I

P

P

E

Q

C
|
C

G

G

E

E

C
|
C

K

K

F

F

C
|
C

K

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q

Q

K

K

I

I

R
|
R

E

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

T

S

R

R

F

F

H

T

K

C

D

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

L

V

A

A

K

K

V

I

N

D

K

P

D

L

A

A

P

P

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

M

Y

G

G

A

A

V

A

M

V

N

N

T

T

A

A

K

K

V

L

E

E

E

P

G

G

A

S

T

V

V

C

A

A

I

V

F

F

G
|
G

I

L

G

G

G
|
G

I
x
V

G

G

L

L

S

A

A

V

I

I

I

M

G

G

A

C

T

K

M

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

V

D
|
D

I
|
I

N

N

E

K

S

D

K
|
K

F

F

E

A

L

R

A

A

R

K

K

E

L

F

G

G

A

A

T

T

D

E

C

C

I

I

N

N

P

P

K

Q

D

D

Y

F

D

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

L

M

T

T

D

D

G

G

V

V

D

D

Y

Y

S

S

F

F

E

E

C
|
C

I
|
I

G

G

N

N

V

V

N

K

V

V

M

M

R

R

S

A

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I
x
V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A
|
A

F

F

G

G

V

W

K

K

G

S

R

V

S

E

E

S

L

V

P

P

D

K

F

L

V

V

E

S

R

E

Y

Y

M

M

K

S

G

K

E

K

F

I

K

K

L

V

D

D

D

E

F

F

I

V

T

T

H

H

T

N

M

L

T

S

L

F

E

D

E

E

I

I

N

N

T

K

A

A

F

F

D

E

L

L

M

M

H

H

E

S

G

G

K

K

S

S

I

I

R
|
R

S

T

V

V

I

V

active site: C44 (= C45), H45 (= H46), T46 (= T47), Y49 (≠ F50), H66 (= H67), E67 (= E68), C96 (= C97), C99 (= C100), C102 (= C103), C110 (= C111), R114 (= R115), C173 (= C174), T177 (= T178), R368 (= R369)
binding 2-amino-4-[1-carboxymethyl-carbamoyl)-2-hydroxymethylsulfanyl-ethylcarbamoyl]-butyric acid: T46 (= T47), P56 (= P57), E57 (= E58), H66 (= H67), Y92 (= Y93), R114 (= R115), C173 (= C174), V293 (= V294)
binding nicotinamide-adenine-dinucleotide: C44 (= C45), H45 (= H46), T46 (= T47), C173 (= C174), T177 (= T178), G198 (= G199), G201 (= G202), V202 (≠ I203), D222 (= D223), I223 (= I224), K227 (= K228), C267 (= C268), I268 (= I269), V291 (≠ I292), G292 (= G293), V293 (= V294), A317 (= A318), R368 (= R369)
binding zinc ion: C44 (= C45), H66 (= H67), C96 (= C97), C99 (= C100), C102 (= C103), C110 (= C111), C173 (= C174)

1ma0A Ternary complex of human glutathione-dependent formaldehyde dehydrogenase with NAD+ and dodecanoic acid (see paper)
65% identity, 98% coverage: 3:372/376 of query aligns to 2:371/373 of 1ma0A

query
sites
1ma0A

N

N

K

E

T

V

I

I

K

K

S

C

K

K

A

A

I

V

A

A

W

W

G

E

P

A

N

G

Q

K

P

P

L

L

S

S

I

I

E

E

V

I

D

E

V

V

M

A

P

P

Q

K

K

A

G

H

E

E

V

V

R

R

V

I

R

K

I

I

V

I

A

A

S

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

L

S

S

G

G

D

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

I

I

L

L

G

G

H
|
H

E
|
E

G

G

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A

K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

I

P

P

E

Q

C
|
C

G

G

E

E

C
|
C

K

K

F

F

C
|
C

K

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q

Q

K

K

I

I

R
|
R

E

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

T

S

R

R

F

F

H

T

K

C

D

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

L

V

A

A

K

K

V

I

N

D

K

P

D

L

A

A

P

P

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

M

Y

G

G

A

A

V

A

M

V

N

N

T

T

A

A

K

K

V

L

E

E

E

P

G

G

A

S

T

V

V

C

A

A

I

V

F

F

G

G

I

L

G
|
G

G

G

I
x
V

G

G

L

L

S

A

A

V

I

I

I

M

G

G

A

C

T

K

M

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

V

D
|
D

I
|
I

N

N

E

K

S

D

K

K

F

F

E

A

L

R

A

A

R

K

K

E

L

F

G

G

A

A

T

T

D

E

C

C

I

I

N

N

P

P

K

Q

D

D

Y

F

D

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

L

M

T

T

D

D

G

G

V

V

D

D

Y

Y

S

S

F

F

E

E

C
|
C

I
|
I

G

G

N

N

V

V

N

K

V

V

M

M

R

R

S

A

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I
x
V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A
|
A

F
|
F

G

G

V

W

K

K

G

S

R

V

S

E

E

S

L

V

P

P

D

K

F

L

V

V

E

S

R

E

Y

Y

M

M

K

S

G

K

E

K

F

I

K

K

L

V

D

D

D

E

F

F

I

V

T

T

H

H

T

N

M

L

T

S

L

F

E

D

E

E

I

I

N

N

T

K

A

A

F

F

D

E

L

L

M

M

H

H

E

S

G

G

K

K

S

S

I

I

R
|
R

S

T

V

V

I

V

active site: C44 (= C45), H45 (= H46), T46 (= T47), Y49 (≠ F50), H66 (= H67), E67 (= E68), C96 (= C97), C99 (= C100), C102 (= C103), C110 (= C111), R114 (= R115), C173 (= C174), T177 (= T178), R368 (= R369)
binding nicotinamide-adenine-dinucleotide: H45 (= H46), T177 (= T178), G200 (= G201), V202 (≠ I203), D222 (= D223), I223 (= I224), C267 (= C268), I268 (= I269), V291 (≠ I292), G292 (= G293), V293 (= V294), A317 (= A318), F318 (= F319)
binding zinc ion: C44 (= C45), H66 (= H67), E67 (= E68), C96 (= C97), C99 (= C100), C102 (= C103), C110 (= C111), C173 (= C174)

1m6wA Binary complex of human glutathione-dependent formaldehyde dehydrogenase and 12-hydroxydodecanoic acid (see paper)
65% identity, 98% coverage: 3:372/376 of query aligns to 2:371/373 of 1m6wA

query
sites
1m6wA

N

N

K

E

T

V

I

I

K

K

S

C

K

K

A

A

I

V

A

A

W

W

G

E

P

A

N

G

Q

K

P

P

L

L

S

S

I

I

E

E

V

I

D

E

V

V

M

A

P

P

Q

K

K

A

G

H

E

E

V

V

R

R

V

I

R

K

I

I

V

I

A

A

S

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

L

S

S

G

G

D

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

I

I

L

L

G

G

H
|
H

E
|
E

G

G

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A

K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T

I

P

P

E

Q

C
|
C

G

G

E

E

C
|
C

K

K

F

F

C
|
C

K

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q
|
Q

K

K

I

I

R
|
R

E

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

T

S

R

R

F

F

H

T

K

C

D

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

L

V

A

A

K

K

V

I

N

D

K

P

D

L

A

A

P

P

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

M

Y

G

G

A

A

V

A

M

V

N

N

T

T

A

A

K

K

V

L

E

E

E

P

G

G

A

S

T

V

V

C

A

A

I

V

F

F

G

G

I

L

G

G

I

V

G

G

L

L

S

A

A

V

I

I

I

M

G

G

A

C

T

K

M

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

V

D

D

I

I

N

N

E

K

S

D

K

K

F

F

E

A

L

R

A

A

R

K

K

E

L

F

G

G

A

A

T

T

D

E

C

C

I

I

N

N

P

P

K

Q

D

D

Y

F

D

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

L

M

T

T

D

D

G

G

V

V

D

D

Y

Y

S

S

F

F

E

E

C

C

I

I

G

G

N

N

V

V

N

K

V

V

M

M

R

R

S

A

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

G

G

V

V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

W

K

K

G

S

R

V

S

E

E

S

L

V

P

P

D

K

F

L

V

V

E

S

R

E

Y

Y

M

M

K

S

G

K

E

K

F

I

K

K

L

V

D

D

D

E

F

F

I

V

T

T

H

H

T

N

M

L

T

S

L

F

E

D

E

E

I

I

N

N

T

K

A

A

F

F

D

E

L

L

M

M

H

H

E

S

G

G

K

K

S

S

I

I

R
|
R

S

T

V

V

I

V

active site: C44 (= C45), H45 (= H46), T46 (= T47), Y49 (≠ F50), H66 (= H67), E67 (= E68), C96 (= C97), C99 (= C100), C102 (= C103), C110 (= C111), R114 (= R115), C173 (= C174), T177 (= T178), R368 (= R369)
binding 12-hydroxydodecanoic acid: T46 (= T47), H66 (= H67), Y92 (= Y93), Q111 (= Q112), R114 (= R115), C173 (= C174)
binding zinc ion: C44 (= C45), H66 (= H67), C96 (= C97), C99 (= C100), C102 (= C103), C110 (= C111), C173 (= C174)

P12711 Alcohol dehydrogenase class-3; Alcohol dehydrogenase 2; Alcohol dehydrogenase 5; Alcohol dehydrogenase B2; ADH-B2; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Rattus norvegicus (Rat) (see 2 papers)
64% identity, 99% coverage: 1:374/376 of query aligns to 1:374/374 of P12711

query
sites
P12711

M
|
M

N
x
A

N

N

K

Q

T

V

I

I

K

R

S

C

K

K

A

A

I

V

A

A

W

W

G

E

P

A

N

G

Q

K

P

P

L

L

S

S

I

I

E

E

V

I

D

E

V

V

M

A

P

P

Q

Q

K

A

G

H

E

E

V

V

R

R

V

I

R

K

I

I

V

I

A

A

S

T

G

A

V

V

C

C

H

H

T

T

D

D

A

A

F

Y

T

T

L

L

S

S

G

G

D

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

I

I

L

L

G

G

H

H

E

E

G

G

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A

K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

I

P

P

E

Q

C

C

G

G

E

E

C

C

K

K

F

F

C

C

K

L

S

N

G

P

K

K

T

T

N

N

L

L

C

C

Q

Q

K

K

I

I

R

R

E

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

T

S

R

R

F

F

H

T

K

C

D

K

G

G

Q

K

P

P

I

I

F

L

H

H

Y

F

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

L

V

A

A

K

K

V

I

N

D

K

P

D

S

A

A

P

P

L

L

E

D

E

K

I

V

C

C

L

L

G

G

C

C

G

G

V

I

T

S

T

T

G

G

M

Y

G

G

A

A

V

A

M

V

N

N

T

T

A

A

K

K

V

V

E

E

E

P

G

G

A

S

T

T

V

C

A

A

I

V

F

F

G

G

I

L

G

G

I

V

G

G

L

L

S

A

A

V

I

I

I

M

G

G

A

C

T

K

M

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

I

D

D

I

I

N

N

E

K

S

D

K

K

F

F

E

A

L

K

A

A

R

K

K

E

L

F

G

G

A

A

T

T

D

E

C

C

I

I

N

N

P

P

K

Q

D

D

Y

F

D

S

K

K

P

S

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

L

M

T

T

D

D

G

G

V

V

D

D

Y

F

S

S

F

F

E

E

C

C

I

I

G

G

N

N

V

V

N

K

V

V

M

M

R

R

S

S

A

A

L

L

E

E

C

A

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

G

G

V

V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

W

K

K

G

S

R

V

S

E

E

S

L

V

P

P

D

K

F

L

V

V

E

S

R

E

Y

Y

M

M

K

S

G

K

E

K

F

I

K

K

L

V

D

D

D

E

F

F

I

V

T

T

H

G

T

N

M

L

T

S

L

F

E

D

E

Q

I

I

N

N

T

K

A

A

F

F

D

D

L

L

M

M

H

H

E

S

G

G

K

N

S

S

I

I

R

R

S

T

V

V

I

L

H

K

F

M

M1 (= M1) modified: Initiator methionine, Removed
A2 (≠ N2) modified: N-acetylalanine

P80360 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Myxine glutinosa (Atlantic hagfish) (see 2 papers)
62% identity, 99% coverage: 1:374/376 of query aligns to 3:376/376 of P80360

query
sites
P80360

M

M

N

D

N

G

K

Q

T

V

I

I

K

H

S

C

K

K

A

A

I

V

A

A

W

W

G

E

P

A

N

K

Q

K

P

P

L

L

S

S

I

L

E

E

V

I

D

E

V

V

M

A

P

P

Q

K

K

A

G

H

E

E

V

V

R

R

V

M

R

K

I

V

V

L

A

A

S

T

G

A

V

V

C

C

H

H

T

T

D

D

A

A

F

Y

T

T

L

L

S

S

G

G

D

V

D

D

P

P

E

E

G

G

I

S

F

F

P

P

A

V

I

V

L

L

G

G

H

H

E

E

G

G

A

A

G

G

I

I

V

V

E

E

S

S

I

V

G

G

E

E

G

G

V

V

T

T

S

K

V

F

A

K

V

P

G

G

D

D

H

S

V

V

I

I

P

P

L

L

Y

Y

T

I

P

P

E

Q

C

C

G

G

E

E

C

C

K

K

F

F

C

C

K

L

S

N

G

P

K

K

T

T

N

N

L

L

C

C

Q

Q

K

K

I

I

R

R

E

V

T

T

Q

Q

G

G

K

K

G

G

L

M

M

M

P

P

D

D

G

G

T

T

T

S

R

R

F

L

H

T

K

C

D

R

G

G

Q

K

P

S

I

L

F

Y

H

H

Y

F

M

M

G

G

C

A

S

S

T

T

F

F

S

S

E

E

Y

Y

T

A

V

V

L

V

P

A

E

D

I

I

S

S

L

L

A

C

K

R

V

V

N

A

K

P

D

E

A

A

P

P

L

P

E

D

E

R

I

V

C

C

L

L

G

G

C

C

G

G

V

V

T

S

T

T

G

G

M

Y

G

G

A

A

V

P

M

L

N

N

T

T

A

A

K

K

V

V

E

E

E

P

G

G

A

S

T

T

V

C

A

A

I

I

F

F

G

G

I

L

G

G

G

A

I

V

G

G

L

L

S

A

A

A

I

I

I

M

G

G

A

C

T

R

M

V

A

A

K

G

A

A

S

S

R

R

I

I

A

A

I

I

D

D

I

R

N

N

E

P

S

D

K

K

F

F

E

E

L

K

A

A

R

R

K

I

L

F

G

G

A

A

T

T

D

D

C

C

I

V

N

V

P

P

K

D

D

A

Y

S

D

D

K

K

P

P

I

I

Q

S

D

Q

V

V

I

L

V

G

E

E

L

M

T

T

D

D

G

G

V

L

D

D

Y

Y

S

T

F

F

E

E

C

C

I

V

G

G

N

N

V

V

N

G

V

I

M

M

R

R

S

A

A

A

L

L

E

E

C

S

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

V

I

I

L

G

G

V

V

A

A

G

G

A

G

G

G

Q

Q

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

A

A

A

F

F

G

G

V

W

K

K

G

S

R

V

S

E

E

S

L

V

P

P

D

K

F

L

V

V

E

D

R

D

Y

Y

M

M

K

A

G

G

E

K

F

I

K

M

L

V

D

D

D

E

F

F

I

V

T

S

H

H

T

S

M

L

T

P

L

F

E

D

E

S

I

I

N

N

T

E

A

A

F

F

D

D

L

L

M

M

H

H

E

A

G

G

K

K

S

S

I

I

R

R

S

T

V

V

I

L

H

Q

F

L

Sites not aligning to the query:

1 modified: N-acetylserine

Query Sequence

>WP_054343403.1 NCBI__GCF_001305295.1:WP_054343403.1
MNNKTIKSKAAIAWGPNQPLSIEEVDVMPPQKGEVRVRIVASGVCHTDAFTLSGDDPEGI
FPAILGHEGAGIVESIGEGVTSVAVGDHVIPLYTPECGECKFCKSGKTNLCQKIRETQGK
GLMPDGTTRFHKDGQPIFHYMGCSTFSEYTVLPEISLAKVNKDAPLEEICLLGCGVTTGM
GAVMNTAKVEEGATVAIFGIGGIGLSAIIGATMAKASRIIAIDINESKFELARKLGATDC
INPKDYDKPIQDVIVELTDGGVDYSFECIGNVNVMRSALECCHKGWGESVVIGVAGAGQE
ISTRPFQLVTGRVWRGSAFGGVKGRSELPDFVERYMKGEFKLDDFITHTMTLEEINTAFD
LMHEGKSIRSVIHFDK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory