SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_054558201.1 NCBI__GCF_001306415.1:WP_054558201.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9AT00 Protein TRIGALACTOSYLDIACYLGLYCEROL 3, chloroplastic; ABC transporter I family member 13; ABC transporter ABCI.13; AtABCI13; Non-intrinsic ABC protein 11; AtNAP11 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 94% coverage: 1:240/255 of query aligns to 84:338/345 of Q9AT00

query
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Q9AT00

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F94 (= F11) mutation to A: Reduced ATPase activity and transport properties.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
38% identity, 89% coverage: 1:226/255 of query aligns to 2:224/241 of 4u00A

query
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binding adenosine-5'-diphosphate: F12 (= F11), V17 (= V16), S37 (= S36), G38 (= G37), S39 (= S38), G40 (= G39), K41 (= K40), S42 (≠ T41), T43 (≠ V42)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
35% identity, 93% coverage: 1:237/255 of query aligns to 1:236/240 of 4ymuJ

query
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4ymuJ

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binding adenosine-5'-triphosphate: F11 (= F11), V16 (= V16), S36 (= S36), G37 (= G37), S38 (= S38), G39 (= G39), K40 (= K40), S41 (≠ T41), T42 (≠ V42), E162 (= E163), H194 (= H196)
binding magnesium ion: S41 (≠ T41), E162 (= E163)

7d0aB Acinetobacter mlafedb complex in adp-vanadate trapped vclose conformation (see paper)
33% identity, 94% coverage: 1:240/255 of query aligns to 4:243/263 of 7d0aB

query
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7d0aB

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binding adenosine-5'-diphosphate: S39 (= S36), T41 (≠ S38), G42 (= G39), K43 (= K40), E140 (= E137), S142 (= S139), M145 (= M142)

7d08B Acinetobacter mlafedb complex in atp-bound vtrans1 conformation (see paper)
33% identity, 94% coverage: 1:240/255 of query aligns to 4:243/263 of 7d08B

query
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7d08B

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binding adenosine-5'-triphosphate: R14 (≠ F11), R17 (≠ T14), G40 (= G37), T41 (≠ S38), K43 (= K40), T44 (= T41), T45 (≠ V42), E166 (= E163)

6z5uK Cryo-em structure of the a. Baumannii mlabdef complex bound to appnhp (see paper)
33% identity, 94% coverage: 1:240/255 of query aligns to 2:241/253 of 6z5uK

query
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6z5uK

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L

H

S

K

F

S

N

F
x
R

G

G

D

E

T

R

H

V

V

I

L

Y

K

D

G

N

V

I

S

S

T

L

C

N

F

I

D

R

Q

R

G

G

K

Q

T

I

N

T

L

A

I

I

I

M

G

G

Q

P

S
|
S

G
|
G

S

T

G
|
G

K
|
K

T
|
T

V
x
T

F

L

L

L

K

R

C

L

L

I

L

G

G

G

L

Q

F

L

T

V

P

P

E

D

E

Q

G

G

T

E

I

V

S

L

Y

L

S

D

G

G

Q

K

I

D

Y

I

A

A

D

Q

L

M

T

S

S

R

R

Q

E

E

Q

L

R

F

D

A

L

A

R

R

Q

A

E

R

M

M

G

G

M

M

V

L

F

F

Q
|
Q

G

S

S

G

A

A

L

L

F

F

D

T

S

D

M

M

T

S

V

V

E

Y

E

E

N

N

V

V

M

A

F

F

P

P

M

I

E

R

M

A

F

H

T

T

K

K

T

L

A

S

K

E

S

N

D

L

M

I

Q

A

D

E

R

L

A

V

N

A

E

L

V

K

L

L

Q

E

R

S

V

V

N

G

L

L

V

R

D

G

A

T

H

E

K

Q

K

L

F

M

P

P

S

T

E

E

I

L

S

S

G

G

M

M

Q

N

K

R

R

R

V

V

A

A

I

L

A

A

R

R

A

A

I

I

V

A

M

L

N

D

P

P

K

D

Y

L

L

I

F

M

C

Y

D

D

E
|
E

P

P

N

F

S

A

G

G

L

Q

D

D

P

P

K

I

T

V

A

K

I

G

L

V

I

L

D

T

N

R

L

L

I

I

Q

R

E

S

I

L

T

R

E

E

E

A

F

L

N

D

I

L

T

T

V

I

I

I

N

V

T

S

H
|
H

D

D

M

V

N

P

S

E

V

T

M

L

E

S

I

I

G

A

E

D

K

Y

I

I

V

Y

F

V

L

V

K

A

Q

E

G

G

H

K

K

I

E

Q

W

G

E

E

G

G

T

T

K

P

R

E

E

E

I

L

F

Q

K

A

T

Y

D

A

N

S

K

P

A

F

V

V

T

K

D

Q

F

F

V

L

Y

T

S

G

S

S

binding phosphoaminophosphonic acid-adenylate ester: R12 (≠ F11), S37 (= S36), G38 (= G37), G40 (= G39), K41 (= K40), T42 (= T41), T43 (≠ V42), Q86 (= Q85), E164 (= E163), H197 (= H196)
binding magnesium ion: Q86 (= Q85), E164 (= E163)

3c4jA Abc protein artp in complex with atp-gamma-s
37% identity, 89% coverage: 1:226/255 of query aligns to 3:226/242 of 3c4jA

query
sites
3c4jA

M

M

I

I

E

D

V

V

N

H

E

Q

L

L

H

K

K

K

S

S

F
|
F

G

G

D

S

T

L

H

E

V
|
V

L

L

K

K

G

G

V

I

S

N

T

V

C

H

F

I

D

R

Q

E

G

G

K

E

T

V

N

V

L

V

I

V

I

I

G

G

Q

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

V
x
T

F

F

L

L

K

R

C

C

L

L

L

N

G

L

L

L

F

E

T

D

P

F

E

D

E

E

G

G

T

E

I

I

S

I

Y

I

S

D

G

G

Q

-

I

I

Y

N

A

L

D

K

L

A

T

K

S

D

R

T

E

N

Q

L

R

N

D

K

L

V

R

R

Q

E

E

E

M

V

G

G

M

M

V

V

F

F

Q

Q

G

R

S

F

A

N

L

L

F

F

D

P

S

H

M

M

T

T

V

V

E

L

E

N

N

N

V

I

M

T

F

L

P

A

M

P

E

M

M

K

F

V

T

R

K

K

T

W

A

P

K

R

S

E

D

K

M

A

Q

E

D

A

R

K

A

A

N

M

E

E

V

L

L

L

Q

D

R

K

V

V

N

G

L

L

V

K

D

D

A

K

H

A

K

H

K

A

F

Y

P

P

S

D

E

S

I

L

S

S

G

G

M

Q

Q

A

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

M

M

N

E

P

P

K

K

Y

I

L

M

F

L

C

F

D

D

E

E

P

P

N

T

S

S

G

A

L

L

D

D

P

P

K

E

T

M

A

V

I

G

L

E

I

V

D

L

N

S

L

V

I

M

Q

K

E

Q

I

L

T

A

E

N

E

E

F

-

N

G

I

M

T

T

T

M

V

V

I

V

N

V

T

T

H

H

D

E

M

M

N

G

S

F

V

A

M

R

E

E

I

V

G

G

E

D

K

R

I

V

V

L

F

F

L

M

K

D

Q

G

G

G

H

Y

K

I

E

I

W

E

E

E

G

G

T

K

K

P

R

E

E

D

I

L

F

F

binding phosphothiophosphoric acid-adenylate ester: F13 (= F11), V18 (= V16), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (≠ T41), T44 (≠ V42)

3c41J Abc protein artp in complex with amp-pnp/mg2+
37% identity, 89% coverage: 1:226/255 of query aligns to 3:226/242 of 3c41J

query
sites
3c41J

M

M

I

I

E

D

V

V

N

H

E

Q

L

L

H

K

K

K

S

S

F
|
F

G

G

D

S

T

L

H

E

V
|
V

L

L

K

K

G

G

V

I

S

N

T

V

C

H

F

I

D

R

Q

E

G

G

K

E

T

V

N

V

L

V

I

V

I

I

G

G

Q

P

S
|
S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

V
x
T

F

F

L

L

K

R

C

C

L

L

L

N

G

L

L

L

F

E

T

D

P

F

E

D

E

E

G

G

T

E

I

I

S

I

Y

I

S

D

G

G

Q

-

I

I

Y

N

A

L

D

K

L

A

T

K

S

D

R

T

E

N

Q

L

R

N

D

K

L

V

R

R

Q

E

E

E

M

V

G

G

M

M

V

V

F

F

Q

Q

G

R

S

F

A

N

L

L

F

F

D

P

S

H

M

M

T

T

V

V

E

L

E

N

N

N

V

I

M

T

F

L

P

A

M

P

E

M

M

K

F

V

T

R

K

K

T

W

A

P

K

R

S

E

D

K

M

A

Q

E

D

A

R

K

A

A

N

M

E

E

V

L

L

L

Q

D

R

K

V

V

N

G

L

L

V

K

D

D

A

K

H

A

K

H

K

A

F

Y

P

P

S

D

E

S

I

L

S

S

G

G

M

Q

Q

A

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

M

M

N

E

P

P

K

K

Y

I

L

M

F

L

C

F

D
|
D

E

E

P

P

N

T

S

S

G

A

L

L

D

D

P

P

K

E

T

M

A

V

I

G

L

E

I

V

D

L

N

S

L

V

I

M

Q

K

E

Q

I

L

T

A

E

N

E

E

F

-

N

G

I

M

T

T

T

M

V

V

I

V

N

V

T

T

H

H

D

E

M

M

N

G

S

F

V

A

M

R

E

E

I

V

G

G

E

D

K

R

I

V

V

L

F

F

L

M

K

D

Q

G

G

G

H

Y

K

I

E

I

W

E

E

E

G

G

T

K

K

P

R

E

E

D

I

L

F

F

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F11), V18 (= V16), S38 (= S36), G39 (= G37), G41 (= G39), K42 (= K40), S43 (≠ T41), T44 (≠ V42)
binding magnesium ion: S43 (≠ T41), D163 (= D162)

2olkA Abc protein artp in complex with adp-beta-s
37% identity, 89% coverage: 1:226/255 of query aligns to 3:226/242 of 2olkA

query
sites
2olkA

M

M

I

I

E

D

V

V

N

H

E

Q

L

L

H

K

K

K

S

S

F
|
F

G

G

D

S

T

L

H

E

V
|
V

L

L

K

K

G

G

V

I

S

N

T

V

C

H

F

I

D

R

Q

E

G

G

K

E

T

V

N

V

L

V

I

V

I

I

G

G

Q

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

V
x
T

F

F

L

L

K

R

C

C

L

L

L

N

G

L

L

L

F

E

T

D

P

F

E

D

E

E

G

G

T

E

I

I

S

I

Y

I

S

D

G

G

Q

-

I

I

Y

N

A

L

D

K

L

A

T

K

S

D

R

T

E

N

Q

L

R

N

D

K

L

V

R

R

Q

E

E

E

M

V

G

G

M

M

V

V

F

F

Q

Q

G

R

S

F

A

N

L

L

F

F

D

P

S

H

M

M

T

T

V

V

E

L

E

N

N

N

V

I

M

T

F

L

P

A

M

P

E

M

M

K

F

V

T

R

K

K

T

W

A

P

K

R

S

E

D

K

M

A

Q

E

D

A

R

K

A

A

N

M

E

E

V

L

L

L

Q

D

R

K

V

V

N

G

L

L

V

K

D

D

A

K

H

A

K

H

K

A

F

Y

P

P

S

D

E

S

I

L

S

S

G

G

M

Q

Q

A

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

M

M

N

E

P

P

K

K

Y

I

L

M

F

L

C

F

D

D

E

E

P

P

N

T

S

S

G

A

L

L

D

D

P

P

K

E

T

M

A

V

I

G

L

E

I

V

D

L

N

S

L

V

I

M

Q

K

E

Q

I

L

T

A

E

N

E

E

F

-

N

G

I

M

T

T

T

M

V

V

I

V

N

V

T

T

H

H

D

E

M

M

N

G

S

F

V

A

M

R

E

E

I

V

G

G

E

D

K

R

I

V

V

L

F

F

L

M

K

D

Q

G

G

G

H

Y

K

I

E

I

W

E

E

E

G

G

T

K

K

P

R

E

E

D

I

L

F

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F11), V18 (= V16), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (≠ T41), T44 (≠ V42)

2oljA Abc protein artp in complex with adp/mg2+
37% identity, 89% coverage: 1:226/255 of query aligns to 3:226/242 of 2oljA

query
sites
2oljA

M

M

I

I

E

D

V

V

N

H

E

Q

L

L

H

K

K

K

S

S

F
|
F

G

G

D

S

T

L

H

E

V
|
V

L

L

K

K

G

G

V

I

S

N

T

V

C

H

F

I

D

R

Q

E

G

G

K

E

T

V

N

V

L

V

I

V

I

I

G

G

Q

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

V
x
T

F

F

L

L

K

R

C

C

L

L

L

N

G

L

L

L

F

E

T

D

P

F

E

D

E

E

G

G

T

E

I

I

S

I

Y

I

S

D

G

G

Q

-

I

I

Y

N

A

L

D

K

L

A

T

K

S

D

R

T

E

N

Q

L

R

N

D

K

L

V

R

R

Q

E

E

E

M

V

G

G

M

M

V

V

F

F

Q

Q

G

R

S

F

A

N

L

L

F

F

D

P

S

H

M

M

T

T

V

V

E

L

E

N

N

N

V

I

M

T

F

L

P

A

M

P

E

M

M

K

F

V

T

R

K

K

T

W

A

P

K

R

S

E

D

K

M

A

Q

E

D

A

R

K

A

A

N

M

E

E

V

L

L

L

Q

D

R

K

V

V

N

G

L

L

V

K

D

D

A

K

H

A

K

H

K

A

F

Y

P

P

S

D

E

S

I

L

S

S

G

G

M

Q

Q

A

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

M

M

N

E

P

P

K

K

Y

I

L

M

F

L

C

F

D

D

E

E

P

P

N

T

S

S

G

A

L

L

D

D

P

P

K

E

T

M

A

V

I

G

L

E

I

V

D

L

N

S

L

V

I

M

Q

K

E

Q

I

L

T

A

E

N

E

E

F

-

N

G

I

M

T

T

T

M

V

V

I

V

N

V

T

T

H

H

D

E

M

M

N

G

S

F

V

A

M

R

E

E

I

V

G

G

E

D

K

R

I

V

V

L

F

F

L

M

K

D

Q

G

G

G

H

Y

K

I

E

I

W

E

E

E

G

G

T

K

K

P

R

E

E

D

I

L

F

F

binding adenosine-5'-diphosphate: F13 (= F11), V18 (= V16), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (≠ T41), T44 (≠ V42)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 96% coverage: 8:252/255 of query aligns to 12:255/343 of P30750

query
sites
P30750

H

H

K

Q

S

G

F

T

G

R

D

T

T

I

H

Q

V

A

L

L

K

N

G

N

V

V

S

S

T

L

C

H

F

V

D

P

Q

A

G

G

K

Q

T

I

N

Y

L

G

I

V

I

I

G

G

Q

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

T
x
S

V
x
T

F

L

L

I

K

R

C

C

L

V

L

N

G

L

L

L

F

E

T

R

P

P

E

T

E

E

G

G

T

S

I

V

S

L

Y

V

S

D

G

G

Q

Q

I

E

Y

L

A

T

D

T

L

L

T

S

S

E

R

S

E

E

Q

L

R

T

D

K

L

A

R

R

Q

R

E

Q

M

I

G

G

M

M

V

I

F

F

Q

Q

G

H

S

F

A

N

L

L

F

L

D

S

S

S

M

R

T

T

V

V

E

F

E

G

N

N

V

V

M

A

F

L

P

P

M

L

E

E

M

L

F

-

T

D

K

N

T

T

A

P

K

K

S

D

D

E

M

V

Q

K

D

R

R

R

A

V

N

T

E

E

V

L

L

L

Q

S

R

L

V

V

N

G

L

L

V

G

D

D

A

K

H

H

K

D

K

S

F

Y

P

P

S

S

E

N

I

L

S

S

G

G

M

Q

Q

K

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

M

S

N

N

P

P

K

K

Y

V

L

L

F

L

C

C

D

D

E
|
E

P

A

N

T

S

S

G

A

L

L

D

D

P

P

K

A

T

T

A

T

I

R

L

S

I

I

D

L

N

E

L

L

I

L

Q

K

E

D

I

I

T

N

E

R

F

L

N

G

I

L

T

T

T

I

V

L

I

L

N

I

T

T

H

H

D

E

M

M

N

D

S

V

V

V

M

K

E

R

I

I

G

C

E

D

K

C

I

V

V

A

F

V

L

I

K

S

Q

N

G

G

H

E

K

L

E

I

W

E

E

Q

G

D

T

T

K

V

R

S

E

E

I

V

F

F

K

S

T

H

D

P

N

K

K

T

A

P

V

L

T

A

D

Q

F

K

V

F

Y

I

S

Q

S

S

E

T

L

L

F

H

K

L

K

D

V

I

R

P

Q

E

M

D

Y

Y

I

Q

E

E

40:46 (vs. 36:42, 57% identical) binding ATP
E166 (= E163) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
33% identity, 96% coverage: 8:252/255 of query aligns to 13:256/344 of 6cvlD

query
sites
6cvlD

H

H

K
x
Q

S

G

F

T

G

R

D

T

T
x
I

H

Q

V

A

L

L

K

N

G

N

V

V

S

S

T

L

C

H

F

V

D

P

Q

A

G

G

K

Q

T

I

N

Y

L

G

I

V

I

I

G

G

Q

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

T
x
S

V
x
T

F

L

L

I

K

R

C

C

L

V

L

N

G

L

L

L

F

E

T

R

P

P

E

T

E

E

G

G

T

S

I

V

S

L

Y

V

S

D

G

G

Q

Q

I

E

Y

L

A

T

D

T

L

L

T

S

S

E

R

S

E

E

Q

L

R

T

D

K

L

A

R

R

Q

R

E

Q

M

I

G

G

M

M

V

I

F

F

Q

Q

G

H

S

F

A

N

L

L

F

L

D

S

S

S

M

R

T

T

V

V

E

F

E

G

N

N

V

V

M

A

F

L

P

P

M

L

E

E

M

L

F

-

T

D

K

N

T

T

A

P

K

K

S

D

D

E

M

V

Q

K

D

R

R

R

A

V

N

T

E

E

V

L

L

L

Q

S

R

L

V

V

N

G

L

L

V

G

D

D

A

K

H

H

K

D

K

S

F

Y

P

P

S

S

E
x
N

I

L

S
|
S

G

G

M
x
Q

Q

K

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

M

S

N

N

P

P

K

K

Y

V

L

L

F

L

C

C

D

D

E

Q

P

A

N

T

S

S

G

A

L

L

D

D

P

P

K

A

T

T

A

T

I

R

L

S

I

I

D

L

N

E

L

L

I

L

Q

K

E

D

I

I

T

N

E

R

F

L

N

G

I

L

T

T

T

I

V

L

I

L

N

I

T

T

H
|
H

D

E

M

M

N

D

S

V

V

V

M

K

E

R

I

I

G

C

E

D

K

C

I

V

V

A

F

V

L

I

K

S

Q

N

G

G

H

E

K

L

E

I

W

E

E

Q

G

D

T

T

K

V

R

S

E

E

I

V

F

F

K

S

T

H

D

P

N

K

K

T

A

P

V

L

T

A

D

Q

F

K

V

F

Y

I

S

Q

S

S

E

T

L

L

F

H

K

L

K

D

V

I

R

P

Q

E

M

D

Y

Y

I

Q

E

E

binding phosphothiophosphoric acid-adenylate ester: Q14 (≠ K9), I19 (≠ T14), S41 (= S36), G42 (= G37), A43 (≠ S38), G44 (= G39), K45 (= K40), S46 (≠ T41), T47 (≠ V42), N141 (≠ E137), S143 (= S139), Q146 (≠ M142), H200 (= H196)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
33% identity, 96% coverage: 8:252/255 of query aligns to 13:256/344 of 3tuzC

query
sites
3tuzC

H

H

K
x
Q

S

G

F

T

G

R

D

T

T

I

H

Q

V

A

L

L

K

N

G

N

V

V

S

S

T

L

C

H

F

V

D

P

Q

A

G

G

K

Q

T

I

N

Y

L

G

I

V

I

I

G

G

Q

A

S

S

G
|
G

S

A

G
|
G

K
|
K

T
x
S

V
x
T

F

L

L

I

K

R

C

C

L

V

L

N

G

L

L

L

F

E

T

R

P

P

E

T

E

E

G

G

T

S

I

V

S

L

Y

V

S

D

G

G

Q

Q

I

E

Y

L

A

T

D

T

L

L

T

S

S

E

R

S

E

E

Q

L

R

T

D

K

L

A

R

R

Q

R

E

Q

M

I

G

G

M

M

V

I

F

F

Q

Q

G

H

S

F

A

N

L

L

F

L

D

S

S

S

M

R

T

T

V

V

E

F

E

G

N

N

V

V

M

A

F

L

P

P

M

L

E

E

M

L

F

-

T

D

K

N

T

T

A

P

K

K

S

D

D

E

M

V

Q

K

D

R

R

R

A

V

N

T

E

E

V

L

L

L

Q

S

R

L

V

V

N

G

L

L

V

G

D

D

A

K

H

H

K

D

K

S

F

Y

P

P

S

S

E

N

I

L

S

S

G

G

M

Q

Q

K

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

M

S

N

N

P

P

K

K

Y

V

L

L

F

L

C

C

D

D

E

Q

P

A

N

T

S

S

G

A

L

L

D

D

P

P

K

A

T

T

A

T

I

R

L

S

I

I

D

L

N

E

L

L

I

L

Q

K

E

D

I

I

T

N

E

R

F

L

N

G

I

L

T

T

T

I

V

L

I

L

N

I

T

T

H

H

D

E

M

M

N

D

S

V

V

V

M

K

E

R

I

I

G

C

E

D

K

C

I

V

V

A

F

V

L

I

K

S

Q

N

G

G

H

E

K

L

E

I

W

E

E

Q

G

D

T

T

K

V

R

S

E

E

I

V

F

F

K

S

T

H

D

P

N

K

K

T

A

P

V

L

T

A

D

Q

F

K

V

F

Y

I

S

Q

S

S

E

T

L

L

F

H

K

L

K

D

V

I

R

P

Q

E

M

D

Y

Y

I

Q

E

E

binding adenosine-5'-diphosphate: Q14 (≠ K9), G42 (= G37), G44 (= G39), K45 (= K40), S46 (≠ T41), T47 (≠ V42)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
33% identity, 96% coverage: 8:252/255 of query aligns to 13:256/344 of 3tuiC

query
sites
3tuiC

H

H

K

Q

S

G

F

T

G

R

D

T

T

I

H

Q

V

A

L

L

K

N

G

N

V

V

S

S

T

L

C

H

F

V

D

P

Q

A

G

G

K

Q

T

I

N

Y

L

G

I

V

I

I

G

G

Q

A

S

S

G
|
G

S
x
A

G
|
G

K
|
K

T
x
S

V
x
T

F

L

L

I

K

R

C

C

L

V

L

N

G

L

L

L

F

E

T

R

P

P

E

T

E

E

G

G

T

S

I

V

S

L

Y

V

S

D

G

G

Q

Q

I

E

Y

L

A

T

D

T

L

L

T

S

S

E

R

S

E

E

Q

L

R

T

D

K

L

A

R

R

Q

R

E

Q

M

I

G

G

M

M

V

I

F

F

Q

Q

G

H

S

F

A

N

L

L

F

L

D

S

S

S

M

R

T

T

V

V

E

F

E

G

N

N

V

V

M

A

F

L

P

P

M

L

E

E

M

L

F

-

T

D

K

N

T

T

A

P

K

K

S

D

D

E

M

V

Q

K

D

R

R

R

A

V

N

T

E

E

V

L

L

L

Q

S

R

L

V

V

N

G

L

L

V

G

D

D

A

K

H

H

K

D

K

S

F

Y

P

P

S

S

E

N

I

L

S

S

G

G

M

Q

Q

K

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

M

S

N

N

P

P

K

K

Y

V

L

L

F

L

C

C

D

D

E

Q

P

A

N

T

S

S

G

A

L

L

D

D

P

P

K

A

T

T

A

T

I

R

L

S

I

I

D

L

N

E

L

L

I

L

Q

K

E

D

I

I

T

N

E

R

F

L

N

G

I

L

T

T

T

I

V

L

I

L

N

I

T

T

H

H

D

E

M

M

N

D

S

V

V

V

M

K

E

R

I

I

G

C

E

D

K

C

I

V

V

A

F

V

L

I

K

S

Q

N

G

G

H

E

K

L

E

I

W

E

E

Q

G

D

T

T

K

V

R

S

E

E

I

V

F

F

K

S

T

H

D

P

N

K

K

T

A

P

V

L

T

A

D

Q

F

K

V

F

Y

I

S

Q

S

S

E

T

L

L

F

H

K

L

K

D

V

I

R

P

Q

E

M

D

Y

Y

I

Q

E

E

binding adenosine-5'-diphosphate: G42 (= G37), A43 (≠ S38), G44 (= G39), K45 (= K40), S46 (≠ T41), T47 (≠ V42)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

7chaI Cryo-em structure of p.Aeruginosa mlafebd with amppnp (see paper)
32% identity, 93% coverage: 2:237/255 of query aligns to 4:239/262 of 7chaI

query
sites
7chaI

I

V

E

E

V

L

N

K

E

G

L

L

H

T

K

F

S

K

F
x
R

G

G

D

S

T

R

H

A

V

I

L

F

K

D

G

N

V

I

S

D

T

V

C

R

F

I

D

P

Q

R

G

G

K

K

T

V

N

T

L

G

I

I

I

M

G

G

Q

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

V
x
T

F

L

L

L

K

R

C

L

L

I

L

A

G

S

L

Q

F

L

T

R

P

P

E

S

E

K

G

G

T

E

I

V

S

W

Y

V

S

N

G

G

Q

Q

I

N

Y

L

A

P

D

Q

L

L

T

S

S

R

R

G

E

D

Q

L

R

F

D

D

L

M

R

R

Q

K

E

Q

M

F

G

G

M

V

V

L

F

F

Q

Q

G

S

S

G

A

A

L

L

F

F

D

T

S

D

M

L

T

D

V

V

E

F

E

E

N

N

V

V

M

A

F

F

P

P

M

L

E

R

M

V

F

H

T

T

K

Q

T

L

A

P

K

E

S

E

D

M

M

I

Q

R

D

D

R

I

A

V

N

L

E

M

V

K

L

L

Q

Q

R

A

V

V

N

G

L

L

V

R

D

G

A

A

H

V

K

E

K

L

F

M

P

P

S

D

E

E

I

L

S

S

G

G

M

M

Q

K

K

R

R

R

V

V

A

A

I

L

A

A

R

R

A

A

I

I

V

A

M

L

N

D

P

P

K

Q

Y

I

L

L

F

L

C

Y

D

D

E

E

P

P

N

F

S

V

G

G

L

Q

D

D

P

P

K

I

T

A

A

M

I

G

L

V

I

L

D

V

N

R

L

L

I

I

Q

R

E

L

I

L

T

N

E

D

E

A

F

L

N

G

I

I

T

T

T

S

V

I

I

V

N

V

T

S

H

H

D

D

M

L

N

A

S

E

V

T

M

A

E

S

I

I

G

A

E

D

K

Y

I

I

V

Y

F

I

L

V

K

G

Q

D

G

G

H

R

K

V

E

L

W

G

E

H

G

G

T

T

K

P

R

D

E

V

I

L

F

K

K

E

T

T

D

D

N

D

K

P

A

R

V

I

T

R

D

Q

F

F

V

V

binding phosphoaminophosphonic acid-adenylate ester: R13 (≠ F11), S38 (= S36), G39 (= G37), C40 (≠ S38), G41 (= G39), K42 (= K40), T43 (= T41), T44 (≠ V42)

2d62A Crystal structure of multiple sugar binding transport atp-binding protein
32% identity, 93% coverage: 7:242/255 of query aligns to 12:248/375 of 2d62A

query
sites
2d62A

L

I

H

W

K

K

S

R

F

F

G

G

D

D

T

V

H

T

V

A

L

V

K

K

G

D

V

L

S

S

T

L

C

E

F

I

D

K

Q

D

G

G

K

E

T

F

N

L

L

V

I

L

G

G

Q

P

S

S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

V
x
T

F

T

L

L

K

R

C

M

L

I

L

A

G

G

L

L

F

E

T

E

P

P

E

T

E

R

G

G

T

Q

I

I

S

Y

Y

I

S

E

G

D

Q

N

I

L

Y

V

A

A

D

D

-

P

-

E

-

K

-

G

-

V

L

F

T

V

S

P

R

P

E

K

Q

E

R

R

D

D

L

V

R

-

Q

-

E

-

M

-

G

A

M

M

V

V

F

F

Q

Q

G

S

S

Y

A

A

L

L

F

Y

D

P

S

H

M

M

T

T

V

V

E

Y

E

D

N

N

V

I

M

A

F

F

P

P

M

L

E

K

M

L

F

-

T

R

K

K

T

V

A

P

K

K

S

Q

D

E

M

I

Q

D

D

K

R

R

A

V

N

R

E

E

V

V

L

A

Q

E

R

M

V

L

N

G

L

L

V

T

D

E

A

L

H

L

K

N

K

R

F

K

P

P

S

R

E

E

I

L

S

S

G

G

M

Q

Q

R

K

Q

R

R

V

V

A

A

I

L

A

G

R

R

A

A

I

I

V

I

M

R

N

R

P

P

K

K

Y

V

L

F

F

L

C

M

D

D

E

E

P

P

N

L

S

S

G

N

L

L

D

D

P

A

K

K

T

L

A

R

I

V

L

K

I

M

D

R

N

A

L

E

I

L

Q

K

E

K

I

L

T

Q

E

R

E

Q

F

L

N

G

I

V

T

T

V

I

I

Y

N

V

T

T

H

H

D

D

M

Q

N

V

S

E

V

A

M

M

E

T

I

M

G

G

E

D

K

R

I

I

V

A

F

V

L

M

K

N

Q

K

G

G

H

E

K

L

E

Q

W

Q

E

V

G

G

T

T

K

P

R

D

E

E

I

V

-

Y

F

Y

K

K

T

P

D

V

N

N

K

T

A

F

V

V

T

A

D

G

F

F

V

I

Y

G

S

S

S

P

E

P

L

M

binding pyrophosphate 2-: G42 (= G37), C43 (≠ S38), G44 (= G39), K45 (= K40), T46 (= T41), T47 (≠ V42)

8w6iD Cryo-em structure of escherichia coli str k12 ftsex complex with atp- gamma-s in peptidisc
35% identity, 84% coverage: 1:215/255 of query aligns to 1:214/219 of 8w6iD

query
sites
8w6iD

M

M

I

I

E

R

V

F

N

E

E

H

L

V

H

S

K

K

S

A

F
x
Y

-

L

G

G

D

G

T

R

H

Q

V

A

L

L

K

Q

G

G

V

V

S

T

T

F

C

H

F

M

D

Q

Q

P

G

G

K

E

T

M

N

A

L

F

I

L

I

T

G

G

Q

H

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

T
x
S

V
x
T

F

L

L

L

K

K

C

L

L

I

L

C

G

G

L

I

F

E

T

R

P

P

E

S

E

A

G

G

T

K

I

I

S

W

Y

F

S

S

G

G

Q

H

I

D

Y

I

A

T

D

R

L

L

T

K

S

N

R

R

E

E

Q

V

R

P

D

F

L

L

R

R

Q

R

E

Q

M

I

G

G

M

M

V

I

F

F

Q
|
Q

G

D

S

H

A

H

L

L

F

L

D

M

S

D

M

R

T

T

V

V

E

Y

E

D

N

N

V

V

M

A

F

I

P

P

M

L

E

-

M

I

F

I

T

A

K

G

T

A

A

S

K

G

S

D

D

D

M

I

Q

R

D

R

R

R

A

V

N

S

E

A

V

A

L

L

Q

D

R

K

V

V

N

G

L

L

V

L

D

D

A
x
K

H

A

K

K

K

N

F

F

P

P

S

I

E
x
Q

I

L

S
|
S

G
|
G

M
x
E

Q

Q

K

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

I

V

V

V

M

N

N

K

P

P

K

A

Y

V

L

L

F

L

C

A

D

D

E

E

P

P

N

T

S

G

G

N

L

L

D

D

P

D

K

A

T

-

A

-

I

-

L

-

I

L

D

S

N

E

L

G

I

I

Q

L

E

R

I

L

T

F

E

E

F

F

N

N

-

R

-

V

-

G

I

V

T

T

T

V

V

L

I

M

N

A

T

T

H
|
H

D

D

M

I

N

N

S

L

V

I

M

S

E

R

I

R

G

S

E

Y

K

R

I

M

V

L

F

T

L

L

K

S

Q

D

G

G

H

H

binding phosphothiophosphoric acid-adenylate ester: Y11 (≠ F11), S37 (= S36), G38 (= G37), A39 (≠ S38), G40 (= G39), K41 (= K40), S42 (≠ T41), T43 (≠ V42), Q86 (= Q85), K130 (≠ A130), Q137 (≠ E137), S139 (= S139), G140 (= G140), G141 (= G141), E142 (≠ M142), H195 (= H196)

P0A9R7 Cell division ATP-binding protein FtsE from Escherichia coli (strain K12) (see paper)
35% identity, 84% coverage: 1:215/255 of query aligns to 1:214/222 of P0A9R7

query
sites
P0A9R7

M

M

I

I

E

R

V

F

N

E

E

H

L

V

H

S

K

K

S

A

F

Y

-

L

G

G

D

G

T

R

H

Q

V

A

L

L

K

Q

G

G

V

V

S

T

T

F

C

H

F

M

D

Q

Q

P

G

G

K

E

T

M

N

A

L

F

I

L

I

T

G

G

Q

H

S

S

G

G

S

A

G

G

K
|
K

T

S

V

T

F

L

L

L

K

K

C

L

L

I

L
x
C

G

G

L

I

F

E

T

R

P

P

E

S

E

A

G

G

T

K

I

I

S

W

Y

F

S

S

G

G

Q

H

I

D

Y

I

A

T

D

R

L

L

T

K

S

N

R

R

E

E

Q

V

R

P

D

F

L

L

R

R

Q

R

E

Q

M

I

G

G

M

M

V

I

F

F

Q

Q

G

D

S

H

A

H

L

L

F

L

D

M

S

D

M

R

T

T

V

V

E

Y

E

D

N

N

V

V

M

A

F

I

P

P

M

L

E

-

M

I

F

I

T

A

K

G

T

A

A

S

K

G

S

D

D

D

M

I

Q

R

D

R

R

R

A

V

N

S

E

A

V

A

L

L

Q

D

R

K

V

V

N

G

L

L

V

L

D

D

A

K

H

A

K

K

K

N

F

F

P

P

S

I

E

Q

I

L

S

S

G

G

M

E

Q

Q

K

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

I

V

V

V

M

N

N

K

P

P

K

A

Y

V

L

L

F

L

C

A

D

D

E

E

P

P

N

T

S

G

G

N

L

L

D

D

P

D

K

A

T

-

A

-

I

-

L

-

I

L

D

S

N

E

L

G

I

I

Q

L

E

R

I

L

T

F

E

E

F

F

N

N

-

R

-

V

-

G

I

V

T

T

T

V

V

L

I

M

N

A

T

T

H

H

D

D

M

I

N

N

S

L

V

I

M

S

E

R

I

R

G

S

E

Y

K

R

I

M

V

L

F

T

L

L

K

S

Q

D

G

G

H

H

K41 (= K40) mutation to R: Does not bind ATP.
C49 (≠ L48) mutation to A: Prevents dimer formation. Does not alter ATP-binding.

8g4cB Bceabs atpgs high res tm (see paper)
32% identity, 84% coverage: 1:214/255 of query aligns to 3:219/248 of 8g4cB

query
sites
8g4cB

M

I

I

L

E

E

V

A

N

N

E

K

L

I

H

R

K

K

S

S

F
x
Y

G

G

D

N

T

K

-

L

-

N

-

K

-

Q

H

E

V

V

L

L

K

K

G

G

V

I

S

D

T

I

C

H

F

I

D

E

Q

K

G

G

K

E

T

F

N

V

L

S

I

I

I

M

G

G

Q

A

S
|
S

G

G

S
|
S

G

G

K

K

T
|
T

V

T

F

L

L

L

K

N

C

V

L

L

L

S

G

S

L

I

F

D

T

Q

P

V

E

S

E

H

G

G

T

T

I

I

S

H

Y

I

S

N

G

G

Q

N

I

-

Y

-

A

-

D

D

L

M

T

T

S

A

R

M

E

K

Q

E

R

K

D

Q

L

L

-

A

-

E

-

F

-

R

R

K

Q

Q

E

H

M

L

G

G

M

F

V

I

F

F

Q

Q

G

D

S

Y

A

N

L

L

F

L

D

D

S

T

M

L

T

T

V

V

E

K

E

E

N

N

V

I

M

L

F

L

P

P

M

L

E

S

M

I

F

-

T

T

K

K

T

L

A

S

K

K

S

K

D

E

M

A

Q

N

D

R

R

K

A

F

N

E

E

E

V

V

L

A

Q

K

R

E

V

L

N

G

L

I

V

Y

D

E

A

L

H

R

K

D

K

K

F

Y

P

P

S

N

E

E

I

I

S

S

G

G

M

Q

Q

K

K

Q

R

R

V

T

A

S

I

A

A

G

R

R

A

A

I

F

V

I

M

H

N

D

P

P

K

S

Y

I

L

I

F

F

C

A

D
|
D

E

E

P

P

N

T

S

G

G

A

L

L

D

D

P

S

K

K

T

S

A

A

I

S

L

D

I

L

D

L

N

N

L

K

I

L

Q

S

E

Q

I

L

T

N

E

Q

E

K

F

R

N

N

I

A

T

T

T

I

V

I

I

M

N

V

T

T

H

H

D

D

M

P

N

V

S

A

V

A

M

S

E

Y

I

C

G

G

E

-

K

R

I

V

V

I

F

F

L

I

K

K

Q

D

G

G

binding phosphothiophosphoric acid-adenylate ester: Y13 (≠ F11), S42 (= S36), S44 (= S38), T47 (= T41), D168 (= D162)

7tchB Bceab e169q variant atp-bound conformation (see paper)
32% identity, 84% coverage: 1:215/255 of query aligns to 2:219/245 of 7tchB

query
sites
7tchB

M

I

I

L

E

E

V

A

N

N

E

K

L

I

H

R

K

K

S

S

F
x
Y

G

G

D

N

T

K

-

L

-

N

-

K

-
x
Q

H

E

V
|
V

L

L

K

K

G

G

V

I

S

D

T

I

C

H

F

I

D

E

Q

K

G

G

K

E

T

F

N

V

L

S

I

I

I

M

G

G

Q

A

S
|
S

G
|
G

S
|
S

G

G

K
|
K

T
|
T

V

T

F

L

L

L

K

N

C

V

L

L

L

S

G

S

L

I

F

D

T

Q

P

V

E

S

E

H

G

G

T

T

I

I

S

H

Y

I

S

N

G

G

Q

N

I

-

Y

-

A

-

D

D

L

M

T

T

S

A

R

M

E

K

Q

E

R

K

D

Q

L

L

-

A

-

E

-

F

-

R

R

K

Q

Q

E

H

M

L

G

G

M

F

V

I

F

F

Q

Q

G

D

S

Y

A

N

L

L

F

L

D

D

S

T

M

L

T

T

V

V

E

K

E

E

N

N

V

I

M

L

F

L

P

P

M

L

E

S

M

I

F

-

T

T

K

K

T

L

A

S

K

K

S

K

D

E

M

A

Q

N

D

R

R

K

A

F

N

E

E

E

V

V

L

A

Q

K

R

E

V

L

N

G

L

I

V

Y

D

E

A

L

H

R

K

D

K

K

F

Y

P

P

S

N

E
|
E

I

I

S

S

G
|
G

M

Q

Q

K

K

Q

R

R

V

T

A

S

I

A

A

G

R

R

A

A

I

F

V

I

M

H

N

D

P

P

K

S

Y

I

L

I

F

F

C

A

D

D

E

Q

P

P

N

T

S

G

G

A

L

L

D

D

P

S

K

K

T

S

A

A

I

S

L

D

I

L

D

L

N

N

L

K

I

L

Q

S

E

Q

I

L

T

N

E

Q

E

K

F

R

N

N

I

A

T

T

T

I

V

I

I

M

N

V

T

T

H

H

D

D

M

P

N

V

S

A

V

A

M

S

E

Y

I

C

G

G

E

-

K

R

I

V

V

I

F

F

L

I

K

K

Q

D

G

G

H

Q

binding adenosine-5'-triphosphate: Y12 (≠ F11), Q19 (vs. gap), V21 (= V16), S41 (= S36), G42 (= G37), S43 (= S38), K45 (= K40), T46 (= T41), E142 (= E137), G145 (= G140), G146 (= G141)

Query Sequence

>WP_054558201.1 NCBI__GCF_001306415.1:WP_054558201.1
MIEVNELHKSFGDTHVLKGVSTCFDQGKTNLIIGQSGSGKTVFLKCLLGLFTPEEGTISY
SGQIYADLTSREQRDLRQEMGMVFQGSALFDSMTVEENVMFPMEMFTKTAKSDMQDRANE
VLQRVNLVDAHKKFPSEISGGMQKRVAIARAIVMNPKYLFCDEPNSGLDPKTAILIDNLI
QEITEEFNITTVINTHDMNSVMEIGEKIVFLKQGHKEWEGTKREIFKTDNKAVTDFVYSS
ELFKKVRQMYIEERN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory