SitesBLAST

YI 11:12 (≠ FI 12:13) binding NAD(+)
DNLCNS 31:36 (≠ DNLSNS 32:37) binding NAD(+)
DI 58:59 (≠ DL 59:60) binding NAD(+)
FAGLK 80:84 (≠ FAASK 82:86) binding NAD(+)
N99 (= N101) binding NAD(+)
S124 (= S126) binding NAD(+); mutation to A: No major structural changes. Catalytic efficiency is very low and affinity binding is 21% of the wild-type enzyme.; mutation to T: No major structural changes. Catalytic efficiency is about 30% of that of the wild-type enzyme, and affinity binding is similar to that of the native enzyme.
Y149 (= Y151) active site, Proton acceptor; binding NAD(+); mutation to F: No major structural changes. Catalytic efficiency is very low and affinity binding is 12% of the wild-type enzyme.
K153 (= K155) binding NAD(+); mutation to A: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.; mutation to M: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.
F178 (= F180) binding NAD(+)
Y299 (≠ A304) mutation to C: Loss of epimerase activity with UDP-Gal by almost 5-fold, but it results in a gain of epimerase activity with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) by 230-fold with minimal changes in its three-dimensional structure.

7xpqA Crystal structure of udp-glc/glcnac 4-epimerase with NAD/udp-glcnac (see paper)
48% identity, 97% coverage: 1:336/346 of query aligns to 1:337/343 of 7xpqA

query
sites
7xpqA

M

L

K

R

K

T

V

I

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

Q

L

L

Q

L

N

Q

E

Q

G

G

F

F

E

R

V

V

I

V

V

V

D
|
D

N
|
N

L

L

S

D

N
|
N

S

A

S

S

E

E

N

A

V

A

L

L

D

A

G

R

I

V

I

A

A

E

I

L

T

A

G

G

K

H

K

D

P

G

-

A

-

N

-

L

V

V

F

F

E

H

N

K

F

V

D
|
D

L
|
L

R

R

D

D

K

R

P

H

K

A

V

L

V

V

E

D

F

I

F

F

A

S

K

S

H

H

H

R

D

-

I

F

A

E

G

A

V

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

H

N

K

P

P

L

L

Y

Y

E

D

N
|
N

N

N

L

L

G

V

V

G

L

T

V

I

Y

T

I

L

L

L

Q

E

E

V

L

M

Q

A

K

A

K

N

G

G

E

C

S

K

N

K

F

L

I

V

F

F

S
|
S

S

S

S
|
S

C

A

T

T

V

V

Y

Y

G

G

Q

W

A

P

D

K

T

E

M

V

P

P

I

C

T

T

E

E

D

E

A

F

P

P

V

L

K

-

P

C

A

A

E

T

S

N

P

P

Y
|
Y

G

G

N

R

T

T

K
|
K

Q

L

V

V

G

I

E

E

E

D

I

I

I

C

R

R

D

D

T

V

C

H

K

R

V

S

N

D

P

P

G

D

L

W

A

K

A

I

I

I

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

I

V

G

G

A

A

H

H

P

P

T

S

V

G

E

Y

I

I

G

G

E

E

L

D

P

P

I

C

G

G

V

V

P

P

Q

N

N
|
N

L
|
L

L

M

P

P

F

Y

I

V

T

Q

Q

Q

T

V

G

A

I

V

G

G

M

R

R

L

E

P

Q

H

L

L

S
x
T

V

V

F
x
Y

G

G

D

T

D

D

Y

Y

P

S

T

T

P

K

D

D

G

G

T

T

C

G

I

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

K

D

A

G

H

H

V

I

A

A

L

L

Q

R

R

K

L

L

L

Y

T

E

A

D

K

S

N

D

D

K

T

I

N

G

F

C

E

E

V

V

F

Y

N

N

L

L

G

G

T

T

G

G

T

K

G

G

S

T

S

S

V
|
V

L

L

E

E

V

M

I

V

Q

A

S

A

F

F

E

E

R

K

V

A

S

S

G

G

E

K

K

K

L

I

N

P

Y

L

R

V

I

F

A

A

P

G

R

R

R
|
R

E

P

G

G

D
|
D

V

A

I

E

Q

I

A

V

Y

Y

A

A

D

A

T

T

A

A

K

K

A

A

N

E

E

K

V

E

L

L

G

K

W

W

K

K

A

A

A

K

S

Y

T

G

L

I

D

E

A

M

M

C

E

R

S

D

A

L

W

W

N

N

W

W

E

A

K

S

K

K

binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), Y12 (≠ F12), I13 (= I13), D32 (= D32), N33 (= N33), N36 (= N36), D62 (= D59), L63 (= L60), F84 (= F82), A85 (= A83), G86 (≠ A84), K88 (= K86), N103 (= N101), S126 (= S124), S128 (= S126), Y152 (= Y151), K156 (= K155), Y180 (= Y179), P183 (= P182)
binding uridine-diphosphate-n-acetylglucosamine: N182 (= N181), N202 (= N201), L203 (= L202), T219 (≠ S218), Y221 (≠ F220), R234 (= R233), Y236 (= Y235), V275 (= V274), R298 (= R297), D301 (= D300)

7xpoA Crystal structure of udp-glc/glcnac 4-epimerase with NAD/udp-glc (see paper)
48% identity, 97% coverage: 1:336/346 of query aligns to 1:337/344 of 7xpoA

query
sites
7xpoA

M

L

K

R

K

T

V

I

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

Q

L

L

Q

L

N

Q

E

Q

G

G

F

F

E

R

V

V

I

V

V

V

D
|
D

N
|
N

L

L

S

D

N
|
N

S

A

S

S

E

E

N

A

V

A

L

L

D

A

G

R

I

V

I

A

A

E

I

L

T

A

G

G

K

H

K

D

P

G

-

A

-

N

-

L

V

V

F

F

E

H

N

K

F

V

D
|
D

L
|
L

R

R

D

D

K

R

P

H

K

A

V

L

V

V

E

D

F

I

F

F

A

S

K

S

H

H

H

R

D

-

I

F

A

E

G

A

V

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

H

N

K

P

P

L

L

Y

Y

E

D

N
|
N

N

N

L

L

G

V

V

G

L

T

V

I

Y

T

I

L

L

L

Q

E

E

V

L

M

Q

A

K

A

K

N

G

G

E

C

S

K

N

K

F

L

I

V

F

F

S
|
S

S

S

S
|
S

C
x
A

T

T

V

V

Y

Y

G

G

Q

W

A

P

D

K

T

E

M

V

P

P

I

C

T

T

E

E

D

E

A

F

P

P

V

L

K

-

P

C

A

A

E

T

S

N

P

P

Y
|
Y

G

G

N

R

T

T

K
|
K

Q

L

V

V

G

I

E

E

E

D

I

I

I

C

R

R

D

D

T

V

C

H

K

R

V

S

N

D

P

P

G

D

L

W

A

K

A

I

I

I

A

L

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

I

V

G

G

A

A

H

H

P

P

T

S

V

G

E

Y

I

I

G

G

E

E

L

D

P

P

I

C

G

G

V

V

P

P

Q

N

N
|
N

L
|
L

L

M

P

P

F

Y

I

V

T

Q

Q

Q

T

V

G

A

I

V

G

G

M

R

R

L

E

P

Q

H

L

L

S
x
T

V
|
V

F
x
Y

G

G

D

T

D

D

Y

Y

P

S

T

T

P

K

D

D

G

G

T

T

C

G

I

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

K

D

A

G

H

H

V

I

A

A

L

L

Q

R

R

K

L

L

L

Y

T

E

A

D

K

S

N

D

D

K

T

I

N

G

F

C

E

E

V

V

F

Y

N

N

L

L

G

G

T

T

G

G

T

K

G

G

S

T

S

S

V
|
V

L

L

E

E

V

M

I

V

Q

A

S

A

F

F

E

E

R

K

V

A

S

S

G

G

E

K

K

K

L

I

N

P

Y

L

R

V

I

F

A

A

P

G

R

R

R
|
R

E

P

G

G

D
|
D

V

A

I

E

Q

I

A

V

Y

Y

A

A

D

A

T

T

A

A

K

K

A

A

N

E

E

K

V

E

L

L

G

K

W

W

K

K

A

A

A

K

S

Y

T

G

L

I

D

E

A

M

M

C

E

R

S

D

A

L

W

W

N

N

W

W

E

A

K

S

K

K

binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), Y12 (≠ F12), I13 (= I13), D32 (= D32), N33 (= N33), N36 (= N36), D62 (= D59), L63 (= L60), F84 (= F82), A85 (= A83), G86 (≠ A84), K88 (= K86), N103 (= N101), S126 (= S124), S128 (= S126), Y152 (= Y151), K156 (= K155), Y180 (= Y179), P183 (= P182)
binding uridine-5'-diphosphate-glucose: S128 (= S126), A129 (≠ C127), F181 (= F180), N182 (= N181), N202 (= N201), L203 (= L202), T219 (≠ S218), V220 (= V219), Y221 (≠ F220), R234 (= R233), Y236 (= Y235), V275 (= V274), R298 (= R297), D301 (= D300)

1udaA Structure of udp-galactose-4-epimerase complexed with udp-4-deoxy-4- fluoro-alpha-d-galactose (see paper)
46% identity, 98% coverage: 3:340/346 of query aligns to 2:335/338 of 1udaA

query
sites
1udaA

K

R

V

V

L

L

V

V

T

T

G
|
G

G

G

L

S

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

C

V

V

E

Q

L

L

Q

L

N

Q

E

N

G

G

F

H

E

D

V

V

V

I

I

I

V

L

D
|
D

N
|
N

L
|
L

S
x
C

N
|
N

S
|
S

S

K

E

R

N

S

V

V

L

L

D

P

G

V

I

I

I

E

A

R

I

L

T

G

G

G

K

K

K

H

P

P

V

T

F

F

E

V

N

E

F

G

D
|
D

L
x
I

R

R

D

N

K

E

P

A

K

L

V

M

V

T

E

E

F

I

F

L

A

H

K

D

H

H

H

-

D

A

I

I

A

D

G

T

V

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

E

Y

Y

E

D

N

N

L

V

G

N

V

G

L

T

V

L

Y

R

I

L

L

I

Q

S

E

A

L

M

Q

R

K

A

G

N

E

V

S

K

N

N

F

F

I

I

F

F

S
|
S

S

S

S
|
S

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

D

A

N

D

P

T

K

M

I

P

P

I

Y

T

V

E

E

D

S

A

F

P

P

V

T

K

G

P

T

A

P

E

Q

S

S

P

P

Y
|
Y

G

G

N

K

T

S

K
|
K

Q

L

V

M

G

V

E

E

E

Q

I

I

I

L

R

T

D

D

T

L

C

Q

K

K

V

A

N

Q

P

P

G

D

L

W

A

S

A

I

I

A

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

I

V

G

G

A

A

H

H

P

P

T

S

V

G

E

D

I
x
M

G

G

E

E

L

D

P

P

I

Q

G

G

V

I

P

P

Q

N

N
|
N

L
|
L

L

M

P

P

F

Y

I

I

T

A

Q

Q

T

V

G

A

I

V

G

G

M

R

R

R

E

D

Q

S

L

L

S
x
A

V
x
I

F
|
F

G

G

D

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

C

G

I

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

K

D

A

G

H

H

V

V

A

V

A

A

L

M

Q

E

R

K

L

L

L

-

T

-

A

-

K

A

N

N

D

K

T

P

N

G

F

V

E

H

V

I

F

Y

N

N

L

L

G

G

T

A

G

G

T

V

G

G

S

N

S

S

V

V

L

L

E

D

V

V

I

V

Q

N

S

A

F

F

E

S

R

K

V

A

S

C

G

G

E

K

K

P

L

V

N

N

Y

Y

R

H

I

F

A

A

P

P

R

R

R
|
R

E

E

G

G

D
|
D

V

L

I

P

Q

A

A
x
Y

Y

W

A

A

D

D

T

A

A

S

K

K

A

A

N

D

E

R

V

E

L

L

G

N

W

W

K

R

A

V

A

T

S

R

T

T

L

L

D

D

D

E

A

M

M

A

E

Q

S

D

A

T

W

W

N

H

W

W

E

Q

K

S

K

R

I

H

R

P

K

Q

G

G

active site: S124 (= S126), A125 (≠ C127), T126 (= T128), Y149 (= Y151), K153 (= K155), M189 (≠ I191)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ F12), I12 (= I13), D31 (= D32), N32 (= N33), L33 (= L34), C34 (≠ S35), N35 (= N36), S36 (= S37), D58 (= D59), I59 (≠ L60), F80 (= F82), A81 (= A83), G82 (≠ A84), K84 (= K86), S122 (= S124), Y149 (= Y151), K153 (= K155), Y177 (= Y179), P180 (= P182)
binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: T126 (= T128), N179 (= N181), N199 (= N201), L200 (= L202), A216 (≠ S218), I217 (≠ V219), F218 (= F220), R231 (= R233), Y233 (= Y235), R292 (= R297), D295 (= D300), Y299 (≠ A304)

1naiA Udp-galactose 4-epimerase from escherichia coli, oxidized (see paper)
46% identity, 98% coverage: 3:340/346 of query aligns to 2:335/338 of 1naiA

query
sites
1naiA

K

R

V

V

L

L

V

V

T

T

G
|
G

G

G

L

S

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

C

V

V

E

Q

L

L

Q

L

N

Q

E

N

G

G

F

H

E

D

V

V

V

I

I

I

V

L

D
|
D

N
|
N

L
|
L

S
x
C

N
|
N

S
|
S

S

K

E

R

N

S

V

V

L

L

D

P

G

V

I

I

I

E

A

R

I

L

T

G

G

G

K

K

K

H

P

P

V

T

F

F

E

V

N

E

F

G

D
|
D

L
x
I

R

R

D

N

K

E

P

A

K

L

V

M

V

T

E

E

F

I

F

L

A

H

K

D

H

H

H

-

D

A

I

I

A

D

G

T

V

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

E

Y

Y

E

D

N

N

L

V

G

N

V

G

L

T

V

L

Y

R

I

L

L

I

Q

S

E

A

L

M

Q

R

K

A

G

N

E

V

S

K

N

N

F

F

I

I

F

F

S

S

S
|
S

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

D

A

N

D

P

T

K

M

I

P

P

I

Y

T

V

E

E

D

S

A

F

P

P

V

T

K

G

P

T

A

P

E

Q

S

S

P

P

Y
|
Y

G

G

N

K

T

S

K
|
K

Q

L

V

M

G

V

E

E

E

Q

I

I

I

L

R

T

D

D

T

L

C

Q

K

K

V

A

N

Q

P

P

G

D

L

W

A

S

A

I

I

A

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

I

V

G

G

A

A

H

H

P

P

T

S

V

G

E

D

I
x
M

G

G

E
|
E

L

D

P
|
P

I

Q

G

G

V

I

P

P

Q

N

N
|
N

L
|
L

L

M

P

P

F

Y

I

I

T

A

Q

Q

T

V

G

A

I

V

G

G

M

R

R

R

E

D

Q

S

L
|
L

S
x
A

V

I

F

F

G

G

D

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

C

G

I

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

K

D

A

G

H

H

V

V

A

V

A

A

L

M

Q

E

R

K

L

L

L

-

T

-

A

-

K

A

N

N

D

K

T

P

N

G

F

V

E

H

V

I

F

Y

N

N

L

L

G

G

T

A

G

G

T

V

G

G

S

N

S

S

V

V

L

L

E

D

V

V

I

V

Q

N

S

A

F

F

E

S

R

K

V

A

S

C

G

G

E

K

K

P

L

V

N

N

Y

Y

R

H

I

F

A

A

P

P

R

R

R
|
R

E

E

G

G

D

D

V

L

I

P

Q

A

A

Y

Y

W

A

A

D

D

T

A

A

S

K

K

A

A

N

D

E

R

V

E

L

L

G

N

W

W

K

R

A

V

A

T

S

R

T

T

L

L

D

D

D

E

A

M

M

A

E

Q

S

D

A

T

W

W

N

H

W

W

E

Q

K

S

K

R

I

H

R

P

K

Q

G

G

active site: S124 (= S126), A125 (≠ C127), T126 (= T128), Y149 (= Y151), K153 (= K155), M189 (≠ I191)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ F12), I12 (= I13), D31 (= D32), N32 (= N33), L33 (= L34), C34 (≠ S35), N35 (= N36), S36 (= S37), D58 (= D59), I59 (≠ L60), F80 (= F82), A81 (= A83), G82 (≠ A84), K84 (= K86), Y149 (= Y151), K153 (= K155), Y177 (= Y179), P180 (= P182)
binding 1,3-propandiol: N35 (= N36), K84 (= K86), E191 (= E193), P193 (= P195)
binding uridine-5'-diphosphate: N179 (= N181), N199 (= N201), L200 (= L202), L215 (= L217), A216 (≠ S218), R231 (= R233), Y233 (= Y235), R292 (= R297)

1lrjA Crystal structure of e. Coli udp-galactose 4-epimerase complexed with udp-n-acetylglucosamine (see paper)
46% identity, 98% coverage: 3:340/346 of query aligns to 2:335/338 of 1lrjA

query
sites
1lrjA

K

R

V

V

L

L

V

V

T

T

G
|
G

G

G

L

S

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

C

V

V

E

Q

L

L

Q

L

N

Q

E

N

G

G

F

H

E

D

V

V

V

I

I

I

V

L

D
|
D

N
|
N

L
|
L

S
x
C

N
|
N

S
|
S

S

K

E

R

N

S

V

V

L

L

D

P

G

V

I

I

I

E

A

R

I

L

T

G

G

G

K

K

K

H

P

P

V

T

F

F

E

V

N

E

F

G

D
|
D

L
x
I

R

R

D

N

K

E

P

A

K

L

V

M

V

T

E

E

F

I

F

L

A

H

K

D

H

H

H

-

D

A

I

I

A

D

G

T

V

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V
|
V

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

E

Y

Y

E

D

N

N

L

V

G

N

V

G

L

T

V

L

Y

R

I

L

L

I

Q

S

E

A

L

M

Q

R

K

A

G

N

E

V

S

K

N

N

F

F

I

I

F

F

S

S

S
|
S

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

D

A

N

D

P

T

K

M

I

P

P

I

Y

T

V

E

E

D

S

A

F

P

P

V

T

K

G

P

T

A

P

E

Q

S

S

P

P

Y
|
Y

G

G

N

K

T

S

K
|
K

Q

L

V

M

G

V

E

E

E

Q

I

I

I

L

R

T

D

D

T

L

C

Q

K

K

V

A

N

Q

P

P

G

D

L

W

A

S

A

I

I

A

A

L

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

I

V

G

G

A

A

H

H

P

P

T

S

V

G

E

D

I
x
M

G

G

E

E

L

D

P

P

I

Q

G

G

V

I

P

P

Q

N

N

N

L
|
L

L

M

P

P

F

Y

I

I

T

A

Q

Q

T

V

G

A

I

V

G

G

M

R

R

R

E

D

Q

S

L
|
L

S
x
A

V

I

F
|
F

G

G

D

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

C

G

I

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

K

D

A

G

H

H

V

V

A

V

A

A

L

M

Q

E

R

K

L

L

L

-

T

-

A

-

K

A

N

N

D

K

T

P

N

G

F

V

E

H

V

I

F

Y

N

N

L

L

G

G

T

A

G

G

T

V

G

G

S

N

S

S

V
|
V

L

L

E

D

V

V

I

V

Q

N

S

A

F

F

E

S

R

K

V

A

S

C

G

G

E

K

K

P

L

V

N

N

Y

Y

R

H

I

F

A

A

P

P

R

R

R
|
R

E

E

G

G

D
|
D

V

L

I

P

Q

A

A

Y

Y

W

A

A

D

D

T

A

A

S

K

K

A

A

N

D

E

R

V

E

L

L

G

N

W

W

K

R

A

V

A

T

S

R

T

T

L

L

D

D

D

E

A

M

M

A

E

Q

S

D

A

T

W

W

N

H

W

W

E

Q

K

S

K

R

I

H

R

P

K

Q

G

G

active site: S124 (= S126), A125 (≠ C127), T126 (= T128), Y149 (= Y151), K153 (= K155), M189 (≠ I191)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ F12), I12 (= I13), D31 (= D32), N32 (= N33), L33 (= L34), C34 (≠ S35), N35 (= N36), S36 (= S37), D58 (= D59), I59 (≠ L60), F80 (= F82), A81 (= A83), G82 (≠ A84), K84 (= K86), Y149 (= Y151), K153 (= K155), Y177 (= Y179), P180 (= P182)
binding uridine-diphosphate-n-acetylglucosamine: V86 (= V88), S124 (= S126), F178 (= F180), N179 (= N181), L200 (= L202), L215 (= L217), A216 (≠ S218), F218 (= F220), R231 (= R233), Y233 (= Y235), V269 (= V274), R292 (= R297), D295 (= D300)

1kvrA Udp-galactose 4-epimerase complexed with udp-phenol (see paper)
45% identity, 98% coverage: 3:340/346 of query aligns to 2:335/338 of 1kvrA

query
sites
1kvrA

K

R

V

V

L

L

V

V

T

T

G
|
G

G

G

L

S

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

C

V

V

E

Q

L

L

Q

L

N

Q

E

N

G

G

F

H

E

D

V

V

V

I

I

I

V

L

D
|
D

N
|
N

L

L

S
x
C

N
|
N

S
|
S

S

K

E

R

N

S

V

V

L

L

D

P

G

V

I

I

I

E

A

R

I

L

T

G

G

G

K

K

K

H

P

P

V

T

F

F

E

V

N

E

F

G

D
|
D

L
x
I

R

R

D

N

K

E

P

A

K

L

V

M

V

T

E

E

F

I

F

L

A

H

K

D

H

H

H

-

D

A

I

I

A

D

G

T

V

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

E

Y

Y

E

D

N

N

L

V

G

N

V

G

L

T

V

L

Y

R

I

L

L

I

Q

S

E

A

L

M

Q

R

K

A

G

N

E

V

S

K

N

N

F

F

I

I

F

F

S
|
S

S
x
A

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

D

A

Q

D

P

T

K

M

I

P

P

I

Y

T

V

E

E

D

S

A

F

P

P

V

T

K

G

P

T

A

P

E

Q

S

S

P

P

Y
|
Y

G

G

N

K

T

S

K
|
K

Q

L

V

M

G

V

E

E

E

Q

I

I

I

L

R

T

D

D

T

L

C

Q

K

K

V

A

N

Q

P

P

G

D

L

W

A

S

A

I

I

A

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

I

V

G

G

A

A

H

H

P

P

T

S

V

G

E

D

I
x
M

G

G

E

E

L

D

P

P

I

Q

G

G

V

I

P

P

Q
x
N

N
|
N

L
|
L

L

M

P

P

F

Y

I

I

T

A

Q

Q

T

V

G

A

I

V

G

G

M

R

R

R

E

D

Q

S

L

L

S
x
A

V
x
I

F
|
F

G

G

D

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

C

G

I

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

K

D

A

G

H

H

V

V

A

V

A

A

L

M

Q

E

R

K

L

L

L

-

T

-

A

-

K

A

N

N

D

K

T

P

N

G

F

V

E

H

V

I

F

Y

N

N

L

L

G

G

T

A

G

G

T

V

G

G

S

N

S

S

V
|
V

L

L

E

D

V

V

I

V

Q

N

S

A

F

F

E

S

R

K

V

A

S

C

G

G

E

K

K

P

L

V

N

N

Y

Y

R

H

I

F

A

A

P

P

R

R

R
|
R

E

E

G

G

D
|
D

V

L

I

P

Q

A

A

Y

Y

W

A

A

D

D

T

A

A

S

K

K

A

A

N

D

E

R

V

E

L

L

G

N

W

W

K

R

A

V

A

T

S

R

T

T

L

L

D

D

D

E

A

M

M

A

E

Q

S

D

A

T

W

W

N

H

W

W

E

Q

K

S

K

R

I

H

R

P

K

Q

G

G

active site: A124 (≠ S126), A125 (≠ C127), T126 (= T128), Y149 (= Y151), K153 (= K155), M189 (≠ I191)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ F12), I12 (= I13), D31 (= D32), N32 (= N33), C34 (≠ S35), N35 (= N36), S36 (= S37), D58 (= D59), I59 (≠ L60), F80 (= F82), A81 (= A83), G82 (≠ A84), K84 (= K86), S122 (= S124), S123 (= S125), Y149 (= Y151), K153 (= K155), Y177 (= Y179), P180 (= P182)
binding uridine-5'-diphosphate: N179 (= N181), N198 (≠ Q200), N199 (= N201), L200 (= L202), A216 (≠ S218), I217 (≠ V219), F218 (= F220), R231 (= R233), Y233 (= Y235), V269 (= V274), R292 (= R297), D295 (= D300)

1a9yA Udp-galactose 4-epimerase mutant s124a/y149f complexed with udp- glucose (see paper)
45% identity, 98% coverage: 3:340/346 of query aligns to 2:335/338 of 1a9yA

query
sites
1a9yA

K

R

V

V

L

L

V

V

T

T

G
|
G

G

G

L

S

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

C

V

V

E

Q

L

L

Q

L

N

Q

E

N

G

G

F

H

E

D

V

V

V

I

I

I

V

L

D
|
D

N
|
N

L

L

S
x
C

N
|
N

S
|
S

S

K

E

R

N

S

V

V

L

L

D

P

G

V

I

I

I

E

A

R

I

L

T

G

G

G

K

K

K

H

P

P

V

T

F

F

E

V

N

E

F

G

D
|
D

L
x
I

R

R

D

N

K

E

P

A

K

L

V

M

V

T

E

E

F

I

F

L

A

H

K

D

H

H

H

-

D

A

I

I

A

D

G

T

V

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

E

Y

Y

E

D

N

N

L

V

G

N

V

G

L

T

V

L

Y

R

I

L

L

I

Q

S

E

A

L

M

Q

R

K

A

G

N

E

V

S

K

N

N

F

F

I

I

F

F

S
|
S

S
x
A

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

D

A

Q

D

P

T

K

M

I

P

P

I

Y

T

V

E

E

D

S

A

F

P

P

V

T

K

G

P

T

A

P

E

Q

S

S

P

P

Y
x
F

G

G

N

K

T

S

K
|
K

Q

L

V

M

G

V

E

E

E

Q

I

I

I

L

R

T

D

D

T

L

C

Q

K

K

V

A

N

Q

P

P

G

D

L

W

A

S

A

I

I

A

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

I

V

G

G

A

A

H

H

P

P

T

S

V

G

E

D

I

M

G

G

E

E

L

D

P

P

I

Q

G

G

V

I

P

P

Q

N

N
|
N

L
|
L

L

M

P

P

F

Y

I

I

T

A

Q

Q

T

V

G

A

I

V

G

G

M

R

R

R

E

D

Q

S

L

L

S
x
A

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x
I

F
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F

G

G

D

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

C

G

I

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R
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R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

K

D

A

G

H

H

V

V

A

V

A

A

L

M

Q

E

R

K

L

L

L

-

T

-

A

-

K

A

N

N

D

K

T

P

N

G

F

V

E

H

V

I

F

Y

N

N

L

L

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G

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A

G

G

T

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G

G

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S

S

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V

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L

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N

S

A

F

F

E

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K

V

A

S

C

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G

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K

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L

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N

N

Y

Y

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H

I

F

A

A

P

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R

R

R
|
R

E

E

G

G

D
|
D

V

L

I

P

Q

A

A
x
Y

Y

W

A

A

D

D

T

A

A

S

K

K

A

A

N

D

E

R

V

E

L

L

G

N

W

W

K

R

A

V

A

T

S

R

T

T

L

L

D

D

D

E

A

M

M

A

E

Q

S

D

A

T

W

W

N

H

W

W

E

Q

K

S

K

R

I

H

R

P

K

Q

G

G

active site: A124 (≠ S126), A125 (≠ C127), T126 (= T128), F149 (≠ Y151), K153 (= K155)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), Y11 (≠ F12), I12 (= I13), D31 (= D32), N32 (= N33), C34 (≠ S35), N35 (= N36), S36 (= S37), D58 (= D59), I59 (≠ L60), F80 (= F82), A81 (= A83), G82 (≠ A84), K84 (= K86), S122 (= S124), S123 (= S125), F149 (≠ Y151), K153 (= K155), Y177 (= Y179), P180 (= P182)
binding uridine-5'-diphosphate-glucose: A125 (≠ C127), T126 (= T128), N179 (= N181), N199 (= N201), L200 (= L202), A216 (≠ S218), I217 (≠ V219), F218 (= F220), R231 (= R233), Y233 (= Y235), R292 (= R297), D295 (= D300), Y299 (≠ A304)

Q14376 UDP-glucose 4-epimerase; Galactowaldenase; UDP-N-acetylgalactosamine 4-epimerase; UDP-GalNAc 4-epimerase; UDP-N-acetylglucosamine 4-epimerase; UDP-GlcNAc 4-epimerase; UDP-galactose 4-epimerase; EC 5.1.3.2; EC 5.1.3.7 from Homo sapiens (Human) (see 18 papers)
48% identity, 97% coverage: 2:336/346 of query aligns to 3:339/348 of Q14376

query
sites
Q14376

K

E

K

K

V

V

L

L

V

V

T

T

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

E

L

L

Q

L

N

E

E

A

G

G

F

Y

E

L

V

P

V

V

I

V

V

I

D
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D

N
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N

L
x
F

S
x
H

N
|
N

-

A

-

F

-

R

-

G

S

S

S

L

E

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N

E

V

S

L

L

D

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x
R

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A

E

I

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T

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G

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V

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F

F

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N

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D

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x
I

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K

A

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L

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F

A

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K

K

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Y

H
x
S

D

F

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M

A

A

G

-

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F
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F

A

A

A
x
G

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L

K
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K

A

A

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V

G

G

E

E

S

S

V

V

E

Q

N

K

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P

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L

L
x
D

Y

Y

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N

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N

N

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L

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G

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L

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Q

E

E

I

L

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Q

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K

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G

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N

N

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x
V

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S

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S
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S

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x
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T

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Y

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G

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D

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E

D

A

A

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x
T

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Y

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E

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A

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A
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A

I

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A

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L
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L

R

R

Y
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Y

F
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F

N
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N

P

P

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G

A

A

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H

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C

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I

G

G

E

E

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G

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x
N

N
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L
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L

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S

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Q

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A

I

I

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V

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N

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x
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R

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D

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A

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A

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x
K

L

L

L

-

T

-

A

-

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K

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D

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C

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R

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E

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G

D
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D

V

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I

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Q

A

A
x
C

Y

Y

A

A

D

N

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A

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x
L

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A

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W

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L

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x
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K

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Q

GYI 12:14 (≠ GFI 11:13) binding NAD(+)
DNFHN 33:37 (≠ DNLSN 32:36) binding NAD(+)
N34 (= N33) to S: in GALAC3; peripheral; nearly normal activity towards UDP-galactose; dbSNP:rs121908046
R51 (≠ G44) to W: in THC13; likely pathogenic; decreased UDP-galactose epimerization activity; decreased UDP-N-acetylglucosamine epimerase activity; reduced NAD+ binding; decreased thermal stability
DI 66:67 (≠ DL 59:60) binding NAD(+)
S81 (≠ H74) mutation to R: 5-fold decreased catalytic efficiency for UDP-galactose epimerization.
F88 (= F82) binding NAD(+)
G90 (≠ A84) to E: in GALAC3; 800-fold decrease in UDP-galactose epimerization activity; dbSNP:rs28940882
K92 (= K86) binding NAD(+)
V94 (= V88) to M: in GALAC3; generalized; 30-fold decrease in UDP-galactose epimerization activity; 2-fold decrease in affinity for UDP-galactose; 24% of normal activity with respect to UDP-N-acetylgalactosamine; dbSNP:rs121908047
D103 (≠ L97) to G: in GALAC3; uncertain significance; 7-fold decrease in UDP-galactose epimerization activity; very mild decrease in activity towards UDP-N-acetylgalactosamine; dbSNP:rs28940883
V128 (≠ I122) to M: in THC13; likely pathogenic
S132 (= S126) mutation to A: Loss of activity.
SAT 132:134 (≠ SCT 126:128) binding substrate
T150 (≠ V144) to M: in THC13; likely pathogenic; 3-fold decreased catalytic efficiency for UDP-galactose epimerization
Y157 (= Y151) active site, Proton acceptor; mutation to F: Loss of activity.
K161 (= K155) binding NAD(+)
A180 (= A174) to V: in dbSNP:rs3204468
L183 (= L177) to P: in GALAC3; peripheral; 3-fold decrease in UDP-galactose epimerization activity; dbSNP:rs121908045
Y185 (= Y179) binding NAD(+)
YFN 185:187 (= YFN 179:181) binding substrate
NNL 206:208 (≠ QNL 200:202) binding substrate
L223 (= L217) to P: in THC13; likely pathogenic
NVF 224:226 (≠ SVF 218:220) binding substrate
G237 (≠ C231) to D: in THC13; uncertain significance
R239 (= R233) binding substrate
K257 (≠ R251) to R: in GALAC3; 7-fold decrease in UDP-galactose epimerization activity; does not affect affinity for UDP-galactose; dbSNP:rs28940884
REGD 300:303 (= REGD 297:300) binding substrate
C307 (≠ A304) mutation to Y: No effect on activity towards UDP-galactose. Loss of activity towards UDP-N-acetylgalactosamine.
L313 (≠ K310) to M: in GALAC3; 6-fold decrease in UDP-galactose epimerization activity; very mild decrease in activity towards UDP-N-acetylgalactosamine; dbSNP:rs3180383
G319 (= G316) to E: in GALAC3; nearly normal activity towards UDP-galactose; mild impairment under conditions of substrate limitation; dbSNP:rs28940885
R335 (≠ N332) to H: in GALAC3; 2-fold decrease in UDP-galactose epimerization activity; dbSNP:rs368637540

1ek6A Structure of human udp-galactose 4-epimerase complexed with nadh and udp-glucose (see paper)
48% identity, 97% coverage: 2:336/346 of query aligns to 3:339/346 of 1ek6A

query
sites
1ek6A

K

E

K

K

V

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

E

L

L

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L

N

E

E

A

G

G

F

Y

E

L

V

P

V

V

I

V

V

I

D
|
D

N
|
N

L

F

S
x
H

N
|
N

-

A

-

F

-

R

-

G

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S

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N

E

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A

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I

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D

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K

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M

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A

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-

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A
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A

A
x
G

S

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K
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K

A

A

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V

G

G

E

E

S

S

V

V

E

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N

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P

L

L

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Y

Y

E

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N

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L

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G

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I

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L

L

Q

E

E

I

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Q

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K

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G

G

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N

N

F

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F

F

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S

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x
A

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T

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Y

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A

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E

D

A

A

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Y

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F

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I

E

E

I

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I

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A

N

A

A

I

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A

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L

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R

Y
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Y

F
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F

N
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N

P
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P

I

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G

G

A

A

H

H

P

A

T

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V

G

E

C

I

I

G

G

E

E

L

D

P

P

I

Q

G

G

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I

P

P

Q

N

N
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N

L
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L

L

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P

F

Y

I

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T

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Q

Q

T

V

G

A

I

I

G

G

M

R

R

R

E

E

Q

A

L

L

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x
N

V
|
V

F
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F

G

G

D

N

D

D

Y

Y

P

D

T

T

P

E

D

D

G

G

T

T

C

G

I

V

R
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R

D

D

Y
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Y

I

I

H

H

V

V

D

D

L

L

A

A

K

K

A

G

H

H

V

I

A

A

L

L

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R

R

K

L

L

L

-

T

-

A

-

K

K

N

E

D

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C

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I

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Y

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N

L

L

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G

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V

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V

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Q

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F

M

E

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K

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A

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G

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K

K

K

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K

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V

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A

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R

R
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R

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E

G

G

D
|
D

V

V

I

A

Q

A

A

C

Y

Y

A

A

D

N

T

P

A

S

K

L

A

A

N

Q

E

E

V

E

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L

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W

W

K

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A

A

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T

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L

L

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D

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A

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R

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active site: S132 (= S126), A133 (≠ C127), T134 (= T128), Y157 (= Y151), K161 (= K155)
binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G8), G12 (= G11), Y13 (≠ F12), I14 (= I13), D33 (= D32), N34 (= N33), H36 (≠ S35), N37 (= N36), D66 (= D59), I67 (≠ L60), F88 (= F82), A89 (= A83), G90 (≠ A84), K92 (= K86), S130 (= S124), S131 (= S125), S132 (= S126), Y157 (= Y151), K161 (= K155), Y185 (= Y179), P188 (= P182)
binding uridine-5'-diphosphate-glucose: S132 (= S126), Y157 (= Y151), F186 (= F180), N187 (= N181), N207 (= N201), L208 (= L202), N224 (≠ S218), V225 (= V219), F226 (= F220), R239 (= R233), Y241 (= Y235), V277 (= V274), R300 (= R297), D303 (= D300)

7kn1A Crystal structure of udp-glucose-4-epimerase (gale) from stenotrophomonas maltophila with bound NAD and formylated udp- arabinopyranose
47% identity, 97% coverage: 4:340/346 of query aligns to 5:336/336 of 7kn1A

query
sites
7kn1A

V

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

A

V

C

V

V

E

E

L

L

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Q

N

Q

E

Q

G

G

F

H

E

G

V

V

I

I

V

V

D
|
D

N
x
S

L

L

S
x
C

N
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N

S

S

S

D

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A

N

S

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V

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V

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E

G

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I

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F

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D

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K

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A

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A

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A

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I

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A

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F

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A

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K

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V

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N

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K

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S

C

A

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Y

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G

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N

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Q

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Y

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K

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A

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A

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Y

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G

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A

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A

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G

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E

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P

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x
S

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L

L

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P

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I

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Q

T

V

G

A

I

A

G

G

M

L

R

L

E

P

Q

E

L

V

S
x
Q

V
|
V

F
|
F

G

G

D

D

Y

Y

P

P

T

T

P

H

D

D

G

G

T

T

C

G

I

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

Q

D

D

L

V

A

A

K

S

A

A

H

H

V

V

A

L

A

A

L

L

Q

Q

R

F

L

L

L

-

T

-

A

-

K

R

N

D

D

Q

T

R

N

R

F

S

E

I

V

T

F

L

N

N

L

L

G

G

T

T

G

G

T

Q

G

G

S

H

S

S

V
|
V

L

L

E

E

V

L

I

I

Q

Q

S

A

F

F

E

E

R

L

V

T

S

T

G

G

E

V

K

R

L

V

N

P

Y

F

R

R

I

I

A

V

P

P

R

R

R
|
R

E

D

G

G

D
|
D

V

I

I

A

Q

V

A

S

Y

F

A

A

D

D

T

A

A

S

K

L

A

A

N

L

E

R

V

E

L

L

G

G

W

W

K

K

A

A

A

R

S

H

T

D

L

L

D

T

D

D

A

M

M

C

E

R

S

D

A

T

W

W

N

K

W

W

E

Q

K

R

K

A

I

M

R

S

K

R

G

A

active site: S126 (= S126), Y150 (= Y151), K154 (= K155)
binding nicotinamide-adenine-dinucleotide: G9 (= G8), G12 (= G11), Y13 (≠ F12), I14 (= I13), D33 (= D32), S34 (≠ N33), C36 (≠ S35), N37 (= N36), D60 (= D59), I61 (≠ L60), F82 (= F82), A83 (= A83), A84 (= A84), K86 (= K86), S124 (= S124), S125 (= S125), S126 (= S126), Y150 (= Y151), K154 (= K155), Y178 (= Y179), P181 (= P182)
binding UDP-4-deoxy-4-formamido-beta-L-arabinopyranose: V88 (= V88), S126 (= S126), Y150 (= Y151), N180 (= N181), S199 (≠ Q200), N200 (= N201), L201 (= L202), Q217 (≠ S218), V218 (= V219), F219 (= F220), R232 (= R233), Y234 (= Y235), V270 (= V274), R293 (= R297), D296 (= D300)

1hzjA Human udp-galactose 4-epimerase: accommodation of udp-n- acetylglucosamine within the active site (see paper)
47% identity, 97% coverage: 2:336/346 of query aligns to 2:338/345 of 1hzjA

query
sites
1hzjA

K

E

K

K

V

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

E

L

L

Q

L

N

E

E

A

G

G

F

Y

E

L

V

P

V

V

I

V

V

I

D
|
D

N
|
N

L

F

S
x
H

N
|
N

-

A

-

F

-

R

-

G

S

S

S

L

E

P

N

E

V

S

L

L

D

R

G

R

I

V

I

Q

A

E

I

L

T

T

G

G

K

R

K

S

P

V

V

E

F

F

E

E

N

E

F

M

D
|
D

L
x
I

R

L

D

D

K

Q

P

G

K

A

V

L

V

Q

E

R

F

L

F

F

A

K

K

K

H

Y

H

S

D

F

I

M

A

A

G

-

V

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

D

Y

Y

E

R

N

V

N

N

L

L

G

T

V

G

L

T

V

I

Y

Q

I

L

L

L

Q

E

E

I

L

M

Q

K

K

A

K

H

G

G

E

V

S

K

N

N

F

L

I

V

F

F

S
|
S

S

S

S
|
S

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

N

A

P

D

Q

T

Y

M

L

P

P

I

L

T

D

E

E

D

A

A

H

P

P

V

T

K

G

P

G

A

C

E

T

S

N

P

P

Y
|
Y

G

G

N

K

T

S

K
|
K

Q

F

V

F

G

I

E

E

I

M

I

I

R

R

D

D

T

L

C

C

K

Q

V

A

N

D

P

K

G

T

L

W

A

N

A

V

I

V

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

I

T

G

G

A

A

H

H

P

A

T

S

V

G

E

C

I

I

G

G

E

E

L

D

P

P

I

Q

G

G

V

I

P

P

Q

N

N
|
N

L
|
L

L

M

P

P

F

Y

I

V

T

S

Q

Q

T

V

G

A

I

I

G

G

M

R

R

R

E

E

Q

A

L

L

S
x
N

V
|
V

F
|
F

G

G

D

N

D

D

Y

Y

P

D

T

T

P

E

D

D

G

G

T

T

C

G

I

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

K

K

A

G

H

H

V

I

A

A

L

L

Q

R

R

K

L

L

L

-

T

-

A

-

K

K

N

E

D

Q

T

C

N

G

F

C

E

R

V

I

F

Y

N

N

L

L

G

G

T

T

G

G

T

T

G

G

S

Y

S

S

V
|
V

L

L

E

Q

V

M

I

V

Q

Q

S

A

F

M

E

E

R

K

V

A

S

S

G

G

E

K

K

K

L

I

N

P

Y

Y

R

K

I

V

A

V

P

A

R

R

R
|
R

E

E

G

G

D
|
D

V

V

I

A

Q

A

A

C

Y

Y

A

A

D

N

T

P

A

S

K

L

A

A

N

Q

E

E

V

E

L

L

G

G

W

W

K

T

A

A

S

L

T

G

L

L

D

D

D

R

A

M

M

C

E

E

S

D

A

L

W

W

N

R

W

W

E

Q

K

K

K

Q

active site: S131 (= S126), A132 (≠ C127), T133 (= T128), Y156 (= Y151), K160 (= K155)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), Y12 (≠ F12), I13 (= I13), D32 (= D32), N33 (= N33), H35 (≠ S35), N36 (= N36), D65 (= D59), I66 (≠ L60), F87 (= F82), A88 (= A83), G89 (≠ A84), K91 (= K86), S129 (= S124), S131 (= S126), Y156 (= Y151), K160 (= K155), Y184 (= Y179), P187 (= P182)
binding uridine-diphosphate-n-acetylglucosamine: N186 (= N181), N206 (= N201), L207 (= L202), N223 (≠ S218), V224 (= V219), F225 (= F220), R238 (= R233), Y240 (= Y235), V276 (= V274), R299 (= R297), D302 (= D300)

1i3lA Molecular basis for severe epimerase-deficiency galactosemia: x-ray structure of the human v94m-substituted udp-galactose 4-epimerase (see paper)
47% identity, 97% coverage: 2:336/346 of query aligns to 2:338/347 of 1i3lA

query
sites
1i3lA

K

E

K

K

V

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

E

L

L

Q

L

N

E

E

A

G

G

F

Y

E

L

V

P

V

V

I

V

V

I

D
|
D

N
|
N

L

F

S
x
H

N
|
N

-

A

-

F

-

R

-

G

S

S

S

L

E

P

N

E

V

S

L

L

D

R

G

R

I

V

I

Q

A

E

I

L

T

T

G

G

K

R

K

S

P

V

V

E

F

F

E

E

N

E

F

M

D
|
D

L
x
I

R

L

D

D

K

Q

P

G

K

A

V

L

V

Q

E

R

F

L

F

F

A

K

K

K

H

Y

H

S

D

F

I

M

A

A

G

-

V

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

M

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

D

Y

Y

E

R

N

V

N

N

L

L

G

T

V

G

L

T

V

I

Y

Q

I

L

L

L

Q

E

E

I

L

M

Q

K

K

A

K

H

G

G

E

V

S

K

N

N

F

L

I

V

F

F

S
|
S

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

N

A

P

D

Q

T

Y

M

L

P

P

I

L

T

D

E

E

D

A

A

H

P

P

V

T

K

G

P

G

A

C

E

T

S

N

P

P

Y
|
Y

G

G

N

K

T

S

K
|
K

Q

F

V

F

G

I

E

E

I

M

I

I

R

R

D

D

T

L

C

C

K

Q

V

A

N

D

P

K

G

T

L

W

A

N

A

V

I

V

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

I

T

G

G

A

A

H

H

P

A

T

S

V

G

E

C

I

I

G

G

E

E

L

D

P

P

I

Q

G

G

V

I

P

P

Q

N

N
|
N

L
|
L

L

M

P

P

F

Y

I

V

T

S

Q

Q

T

V

G

A

I

I

G

G

M

R

R

R

E

E

Q

A

L

L

S
x
N

V
|
V

F
|
F

G

G

D

N

D

D

Y

Y

P

D

T

T

P

E

D

D

G

G

T

T

C

G

I

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

K

K

A

G

H

H

V

I

A

A

L

L

Q

R

R

K

L

L

L

-

T

-

A

-

K

K

N

E

D

Q

T

C

N

G

F

C

E

R

V

I

F

Y

N

N

L

L

G

G

T

T

G

G

T

T

G

G

S

Y

S

S

V
|
V

L

L

E

Q

V

M

I

V

Q

Q

S

A

F

M

E

E

R

K

V

A

S

S

G

G

E

K

K

K

L

I

N

P

Y

Y

R

K

I

V

A

V

P

A

R

R

R
|
R

E

E

G

G

D
|
D

V

V

I

A

Q

A

A

C

Y

Y

A

A

D

N

T

P

A

S

K

L

A

A

N

Q

E

E

V

E

L

L

G

G

W

W

K

T

A

A

S

L

T

G

L

L

D

D

D

R

A

M

M

C

E

E

S

D

A

L

W

W

N

R

W

W

E

Q

K

K

K

Q

active site: S131 (= S126), A132 (≠ C127), T133 (= T128), Y156 (= Y151), K160 (= K155)
binding galactose-uridine-5'-diphosphate: N186 (= N181), N206 (= N201), L207 (= L202), N223 (≠ S218), V224 (= V219), F225 (= F220), R238 (= R233), Y240 (= Y235), V276 (= V274), R299 (= R297), D302 (= D300)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), Y12 (≠ F12), I13 (= I13), D32 (= D32), N33 (= N33), H35 (≠ S35), N36 (= N36), D65 (= D59), I66 (≠ L60), F87 (= F82), A88 (= A83), G89 (≠ A84), K91 (= K86), S129 (= S124), S130 (= S125), S131 (= S126), Y156 (= Y151), K160 (= K155), Y184 (= Y179), P187 (= P182)

1i3kA Molecular basis for severe epimerase-deficiency galactosemia: x-ray structure of the human v94m-substituted udp-galactose 4-epimerase (see paper)
47% identity, 97% coverage: 2:336/346 of query aligns to 2:338/347 of 1i3kA

query
sites
1i3kA

K

E

K

K

V

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

E

L

L

Q

L

N

E

E

A

G

G

F

Y

E

L

V

P

V

V

I

V

V

I

D
|
D

N
|
N

L

F

S
x
H

N
|
N

-

A

-

F

-

R

-

G

S

S

S

L

E

P

N

E

V

S

L

L

D

R

G

R

I

V

I

Q

A

E

I

L

T

T

G

G

K

R

K

S

P

V

V

E

F

F

E

E

N

E

F

M

D
|
D

L
x
I

R

L

D

D

K

Q

P

G

K

A

V

L

V

Q

E

R

F

L

F

F

A

K

K

K

H

Y

H

S

D

F

I

M

A

A

G

-

V

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

M

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

D

Y

Y

E

R

N

V

N

N

L

L

G

T

V

G

L

T

V

I

Y

Q

I

L

L

L

Q

E

E

I

L

M

Q

K

K

A

K

H

G

G

E

V

S

K

N

N

F

L

I

V

F

F

S
|
S

S

S

S
|
S

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

N

A

P

D

Q

T

Y

M

L

P

P

I

L

T

D

E

E

D

A

A

H

P

P

V

T

K

G

P

G

A

C

E

T

S

N

P

P

Y
|
Y

G

G

N

K

T

S

K
|
K

Q

F

V

F

G

I

E

E

I

M

I

I

R

R

D

D

T

L

C

C

K

Q

V

A

N

D

P

K

G

T

L

W

A

N

A

V

I

V

A

L

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

I

T

G

G

A

A

H

H

P

A

T

S

V

G

E

C

I

I

G

G

E

E

L

D

P

P

I

Q

G

G

V

I

P

P

Q

N

N
|
N

L
|
L

L

M

P

P

F

Y

I

V

T

S

Q

Q

T

V

G

A

I

I

G

G

M

R

R

R

E

E

Q

A

L

L

S
x
N

V
|
V

F
|
F

G

G

D

N

D

D

Y

Y

P

D

T

T

P

E

D

D

G

G

T

T

C

G

I

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

K

K

A

G

H

H

V

I

A

A

L

L

Q

R

R

K

L

L

L

-

T

-

A

-

K

K

N

E

D

Q

T

C

N

G

F

C

E

R

V

I

F

Y

N

N

L

L

G

G

T

T

G

G

T

T

G

G

S

Y

S

S

V
|
V

L

L

E

Q

V

M

I

V

Q

Q

S

A

F

M

E

E

R

K

V

A

S

S

G

G

E

K

K

K

L

I

N

P

Y

Y

R

K

I

V

A

V

P

A

R

R

R
|
R

E

E

G

G

D
|
D

V

V

I

A

Q

A

A

C

Y

Y

A

A

D

N

T

P

A

S

K

L

A

A

N

Q

E

E

V

E

L

L

G

G

W

W

K

T

A

A

S

L

T

G

L

L

D

D

D

R

A

M

M

C

E

E

S

D

A

L

W

W

N

R

W

W

E

Q

K

K

K

Q

active site: S131 (= S126), A132 (≠ C127), T133 (= T128), Y156 (= Y151), K160 (= K155)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), Y12 (≠ F12), I13 (= I13), D32 (= D32), N33 (= N33), H35 (≠ S35), N36 (= N36), D65 (= D59), I66 (≠ L60), F87 (= F82), A88 (= A83), G89 (≠ A84), K91 (= K86), S129 (= S124), S131 (= S126), Y156 (= Y151), K160 (= K155), Y184 (= Y179), P187 (= P182)
binding uridine-5'-diphosphate-glucose: F185 (= F180), N186 (= N181), N206 (= N201), L207 (= L202), N223 (≠ S218), V224 (= V219), F225 (= F220), R238 (= R233), Y240 (= Y235), V276 (= V274), R299 (= R297), D302 (= D300)

8wovB Crystal structure of arabidopsis thaliana udp-glucose 4-epimerase 2 (atuge2) complexed with udp, g233a mutant (see paper)
48% identity, 96% coverage: 2:333/346 of query aligns to 3:333/341 of 8wovB

query
sites
8wovB

K

K

K

S

V

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

Q

L

L

Q

L

N

E

E

G

G

G

F

Y

E

S

V

A

V

V

I

V

V

V

D
|
D

N
|
N

L

Y

S
x
D

N
|
N

S
|
S

S

S

E

A

N

A

V

S

L

L

D

Q

G

R

I

V

I

K

A

K

I

L

T

A

G

G

K

E

-

N

-

G

-

N

K

R

P

L

V

S

F

F

E

H

N

Q

F

V

D
|
D

L
|
L

R

R

D

D

K

R

P

P

K

A

V

L

V

E

E

K

F

I

F

F

A

S

K

E

H

T

H

-

D

K

I

F

A

D

G

A

V

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

E

N

K

P

P

L

L

Y

Y

E

N

N
|
N

N

N

L

I

G

V

V

G

L

T

V

V

Y

T

I

L

L

L

Q

E

E

V

L

M

Q

A

K

Q

K

Y

G

G

E

C

S

K

N

N

F

L

I

V

F

F

S
|
S

S

S

S
|
S

C

A

T

T

V

V

Y

Y

G

G

Q

W

A

P

D

K

T

E

M

V

P

P

I

C

T

T

E

E

D

E

A

S

P

P

V

I

K

S

P

-

A

A

E

T

S

N

P

P

Y
|
Y

G

G

N

R

T

T

K
|
K

Q

L

V

F

G

I

E

E

I

I

I

C

R

R

D

D

T

V

C

H

K

R

V

S

N

D

P

S

G

E

L

W

A

K

A

I

I

I

A

L

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

I

V

G

G

A

A

H

H

P

P

T

S

V

G

E

Y

I

I

G

G

E

E

L

D

P

P

I

L

G

G

V

V

P

P

Q

N

N
|
N

L
|
L

L

M

P

P

F

Y

I

V

T

Q

Q

Q

T

V

G

A

I

V

G

G

M

R

R

R

E

P

Q

H

L
|
L

S
x
T

V

V

F
|
F

G

G

D

T

D

D

Y

Y

P

K

T

T

P

K

D

D

G

G

T

T

C

A

I

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

K

D

A

G

H

H

V

I

A

A

L

L

Q

R

R

K

L

L

L

D

T

D

A

L

K

K

N

-

D

-

T

I

N

S

F

C

E

E

V

V

F

Y

N

N

L

L

G

G

T

T

G

G

T

N

G

G

S

T

S

S

V

V

L

L

E

E

V

M

I

V

Q

A

S

A

F

F

E

E

R

K

V

A

S

S

G

G

E

K

K

K

L

I

N

P

Y

L

R

V

I

M

A

A

P

G

R

R

R
|
R

E

P

G

G

D
|
D

V

A

I

E

Q

V

A

V

Y

Y

A

A

D

S

T

T

A

E

K

K

A

A

N

E

E

R

V

E

L

L

G

N

W

W

K

K

A

A

A

K

S

N

T

G

L

I

D

E

A

M

M

C

E

R

S

D

A

L

W

W

N

N

W

W

binding nicotinamide-adenine-dinucleotide: G9 (= G8), G12 (= G11), Y13 (≠ F12), I14 (= I13), D33 (= D32), N34 (= N33), D36 (≠ S35), N37 (= N36), S38 (= S37), D63 (= D59), L64 (= L60), F85 (= F82), A86 (= A83), G87 (≠ A84), K89 (= K86), N104 (= N101), S127 (= S124), S129 (= S126), Y153 (= Y151), K157 (= K155), Y181 (= Y179), P184 (= P182)
binding uridine-5'-diphosphate: N183 (= N181), N203 (= N201), L204 (= L202), L219 (= L217), T220 (≠ S218), F222 (= F220), R235 (= R233), Y237 (= Y235), R297 (= R297), D300 (= D300)

3enkA 1.9a crystal structure of udp-glucose 4-epimerase from burkholderia pseudomallei
48% identity, 97% coverage: 4:340/346 of query aligns to 7:338/340 of 3enkA

query
sites
3enkA

V

I

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

A

V

V

E

E

L

L

Q

L

N

A

E

H

G

G

F

Y

E

D

V

V

I

I

V

A

D
|
D

N
|
N

L

L

S
x
V

N
|
N

S
|
S

S

K

E

R

N

E

V

A

L

I

D

A

G

R

I

I

I

E

A

K

I

I

T

T

G

G

K

K

K

T

P

P

V

A

F

F

E

H

N

E

F

T

D
|
D

L
x
V

R

S

D

D

K

E

P

R

K

A

V

L

V

A

E

R

F

I

F

F

A

-

K

D

H

A

H

H

D

P

I

I

A

T

G

A

V

A

I

I

H

H

F
|
F

A
|
A

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

A

N

K

P

P

L

I

L

E

Y

Y

E

R

N
|
N

N

N

L

L

G

D

V

S

L

L

V

L

Y

S

I

L

L

L

Q

R

E

V

L

M

Q

R

K

E

K

R

G

A

E

V

S

K

N

R

F

I

I

V

F

F

S
|
S

C

A

T
|
T

V

V

Y

Y

G

G

Q

V

A

P

D

E

T

R

M

S

P

P

I

I

T

D

E

E

D

T

A

F

P

P

V

L

K

S

P

-

A

A

E

T

S

N

P

P

Y
|
Y

G

G

N

Q

T

T

K
|
K

Q

L

V

M

G

A

E

E

E

Q

I

I

I

L

R

R

D

D

T

V

C

E

K

A

V

A

N

D

P

P

G

S

L

W

A

R

A

V

I

A

A

T

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

I

V

G

G

A

A

H

H

P

E

T

S

V

G

E

L

I

I

G

G

E

E

L

D

P

P

I

A

G

G

V

I

P

P

Q
x
N

N
|
N

L
|
L

L

M

P

P

F

Y

I

V

T

A

Q

Q

T

V

G

A

I

V

G

G

M

K

R

L

E

E

Q

K

L

L

S
x
R

V
|
V

F
|
F

G

G

D

S

D

D

Y

Y

P

P

T

T

P

P

D

D

G

G

T

T

C

G

I

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

K

R

A

G

H

H

V

I

A

A

L

L

Q

D

R

A

L

L

L

-

T

-

A

E

K

R

N

R

D

D

T

A

N

S

F

L

E

T

V

V

F

-

N

N

L

L

G

G

T

T

G

G

T

R

G

G

S

Y

S

S

V
|
V

L

L

E

E

V

V

I

V

Q

R

S

A

F

F

E

E

R

K

V

A

S

S

G

G

E

R

K

A

L

V

N

P

Y

Y

R

E

I

L

A

V

P

A

R

R

R
|
R

E

P

G

G

D
|
D

V

V

I

A

Q

E

A

C

Y

Y

A

A

D

N

T

P

A

A

K

A

A

A

N

A

E

E

V

T

L

I

G

G

W

W

K

K

A

A

A

E

S

R

T

D

L

L

D

E

D

R

A

M

M

C

E

A

S

D

A

H

W

W

N

R

W

W

E

Q

K

E

K

N

I

N

R

P

K

R

G

G

active site: S127 (= S125), S128 (= S126), T130 (= T128), Y152 (= Y151), K156 (= K155)
binding nicotinamide-adenine-dinucleotide: G11 (= G8), G14 (= G11), Y15 (≠ F12), I16 (= I13), D35 (= D32), N36 (= N33), V38 (≠ S35), N39 (= N36), S40 (= S37), D62 (= D59), V63 (≠ L60), F84 (= F82), A85 (= A83), A86 (= A84), K88 (= K86), N103 (= N101), S126 (= S124), S128 (= S126), Y152 (= Y151), K156 (= K155), Y180 (= Y179), P183 (= P182)
binding uridine-5'-diphosphate-glucose: T130 (= T128), N182 (= N181), N201 (≠ Q200), N202 (= N201), L203 (= L202), R219 (≠ S218), V220 (= V219), F221 (= F220), R234 (= R233), Y236 (= Y235), V272 (= V274), R295 (= R297), D298 (= D300)

6k0iA Crystal structure of udp-glucose 4-epimerase from bifidobacterium longum in complex with NAD+ and udp-glc (see paper)
47% identity, 96% coverage: 4:336/346 of query aligns to 3:330/335 of 6k0iA

query
sites
6k0iA

V

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
|
F

I
|
I

G

A

S

T

H

H

T

T

V

D

V

I

E

E

L

L

Q

L

N

N

E

K

G

G

F

Y

E

D

V

V

V

I

I

S

V

V

D
|
D

N
|
N

L

Y

S

G

N
|
N

S
|
S

S

S

E

P

N

V

V

A

L

L

D

E

G

R

I

V

I

E

A

Q

I

I

T

T

G

G

K

K

K

P

P

V

V

K

F

R

E

Y

N

D

F

G

D
|
D

L
x
V

R

R

D

D

K

E

P

A

K

L

V

M

V

E

E

R

F

V

F

F

A

A

K

E

H

N

H

-

D

N

I

I

A

D

G

W

V

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

A

N

K

P

P

L

I

L

E

Y

Y

E

D

N
|
N

N

N

L

L

G

Y

V

S

L

T

V

L

Y

V

I

L

L

L

Q

K

E

V

L

M

Q

K

K

K

K

H

G

N

E

V

S

K

N

K

F

I

I

I

F

F

S
|
S

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

T

A

P

D

K

T

E

M

L

P

P

I

I

T

T

E

E

D

E

A

T

P

P

V

T

K

G

P

G

A

T

E

T

S

N

P

P

Y
|
Y

G

G

N

T

T

S

K
|
K

Q

L

V

F

G

Q

E

E

E

Q

I

I

I

L

R

R

D

D

T

V

C

H

K

V

V

A

N

D

P

P

G

S

L

W

A

T

A

I

I

V

A

L

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

I

V

G

G

A

A

H

H

P

E

T

S

V

G

E

L

I

L

G

G

E

E

L

D

P

P

I

K

G

G

V

I

P

P

Q
x
A

N
|
N

L
|
L

L

T

P

P

F

Y

I

V

T

A

Q

K

T

V

G

A

I

V

G

G

M

E

R

L

E

K

Q

E

L

V

S
x
Q

V
|
V

F
x
Y

G

G

D

D

Y

Y

P

D

T

T

P

P

D

D

G

G

T

T

C

G

I

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

K

K

A

G

H

H

V

V

A

A

A

V

L

I

Q

D

R

H

L

I

L

-

T

-

A

-

K

-

N

D

D

K

T

E

N

G

F

V

E

F

V

V

F

Y

N

N

L

L

G

G

T

T

G

G

T

H

G

G

S

Y

S

S

V
|
V

L

L

E

E

V

V

I

I

Q

K

S

A

F

Y

E

E

R

K

V

A

S

A

G

G

E

H

K

P

L

I

N

P

Y

Y

R

A

I

I

A

K

P

P

R

R

R
|
R

E

P

G

G

D
|
D

V

I

I

A

Q

A

A

C

Y

Y

A

A

D

D

T

A

A

S

K

K

A

A

N

E

E

K

V

E

L

L

G

G

W

W

K

K

A

A

A

E

S

L

T

T

L

I

D

D

A

M

M

A

E

A

S

S

A

S

W

L

N

N

W

W

E

Q

K

T

K

K

active site: S124 (= S126), A125 (≠ C127), T126 (= T128), Y149 (= Y151), K153 (= K155)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (= I13), D31 (= D32), N32 (= N33), N35 (= N36), S36 (= S37), D58 (= D59), V59 (≠ L60), F80 (= F82), A81 (= A83), G82 (≠ A84), K84 (= K86), N99 (= N101), S122 (= S124), S123 (= S125), Y149 (= Y151), K153 (= K155), Y177 (= Y179), P180 (= P182)
binding uridine-5'-diphosphate-glucose: K84 (= K86), S124 (= S126), Y149 (= Y151), F178 (= F180), N179 (= N181), A198 (≠ Q200), N199 (= N201), L200 (= L202), Q216 (≠ S218), V217 (= V219), Y218 (≠ F220), R231 (= R233), Y233 (= Y235), V268 (= V274), R291 (= R297), D294 (= D300)

6k0hA Crystal structure of udp-glucose 4-epimerase from bifidobacterium longum in complex with NAD+ and udp-glcnac (see paper)
47% identity, 96% coverage: 4:336/346 of query aligns to 3:330/335 of 6k0hA

query
sites
6k0hA

V

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
|
F

I
|
I

G

A

S

T

H

H

T

T

V

D

V

I

E

E

L

L

Q

L

N

N

E

K

G

G

F

Y

E

D

V

V

V

I

I

S

V

V

D
|
D

N
|
N

L

Y

S
x
G

N
|
N

S
|
S

S

S

E

P

N

V

V

A

L

L

D

E

G

R

I

V

I

E

A

Q

I

I

T

T

G

G

K

K

K

P

P

V

V

K

F

R

E

Y

N

D

F

G

D
|
D

L
x
V

R

R

D

D

K

E

P

A

K

L

V

M

V

E

E

R

F

V

F

F

A

A

K

E

H

N

H

-

D

N

I

I

A

D

G

W

V

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

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N

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A

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N

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active site: S124 (= S126), A125 (≠ C127), T126 (= T128), Y149 (= Y151), K153 (= K155)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (= I13), D31 (= D32), N32 (= N33), G34 (≠ S35), N35 (= N36), S36 (= S37), D58 (= D59), V59 (≠ L60), F80 (= F82), A81 (= A83), G82 (≠ A84), K84 (= K86), N99 (= N101), S123 (= S125), Y149 (= Y151), K153 (= K155), Y177 (= Y179), P180 (= P182)
binding uridine-diphosphate-n-acetylglucosamine: K84 (= K86), S124 (= S126), Y149 (= Y151), F178 (= F180), N179 (= N181), A198 (≠ Q200), N199 (= N201), L200 (= L202), Q216 (≠ S218), V217 (= V219), Y218 (≠ F220), R231 (= R233), Y233 (= Y235), V268 (= V274), R291 (= R297), D294 (= D300)

Query Sequence

>WP_054559601.1 NCBI__GCF_001306415.1:WP_054559601.1
MKKVLVTGGLGFIGSHTVVELQNEGFEVVIVDNLSNSSENVLDGIIAITGKKPVFENFDL
RDKPKVVEFFAKHHDIAGVIHFAASKAVGESVENPLLYYENNLGVLVYILQELQKKGESN
FIFSSSCTVYGQADTMPITEDAPVKPAESPYGNTKQVGEEIIRDTCKVNPGLAAIALRYF
NPIGAHPTVEIGELPIGVPQNLLPFITQTGIGMREQLSVFGDDYPTPDGTCIRDYIHVVD
LAKAHVAALQRLLTAKNDTNFEVFNLGTGTGSSVLEVIQSFERVSGEKLNYRIAPRREGD
VIQAYADTAKANEVLGWKAASTLDDAMESAWNWEKKIRKGKEKTMA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory