SitesBLAST

G98 (= G11) binding NAD(+)
F99 (= F12) binding NAD(+)
V100 (≠ L13) binding NAD(+)
D119 (= D32) binding NAD(+)
N120 (= N33) binding NAD(+)
F122 (≠ I35) binding NAD(+)
T123 (= T36) binding NAD(+)
G124 (= G37) binding NAD(+)
D144 (= D57) binding NAD(+)
V145 (= V58) binding NAD(+)
L149 (≠ V62) binding UDP-alpha-D-glucuronate
Y150 (≠ H63) binding UDP-alpha-D-glucuronate
L159 (≠ F74) binding NAD(+)
S161 (= S76) binding NAD(+)
K177 (= K92) binding UDP-alpha-D-glucuronate
T178 (≠ V93) binding NAD(+)
N185 (= N100) binding UDP-alpha-D-glucuronate
G188 (= G103) binding UDP-alpha-D-glucuronate
K191 (= K106) binding UDP-alpha-D-glucuronate
R192 (≠ D107) binding UDP-alpha-D-glucuronate
A200 (= A115) binding NAD(+)
E204 (= E119) mutation to A: Reduced UDP-glucuronic acid decarboxylase activity.
Y231 (= Y146) active site, Proton acceptor; binding NAD(+); mutation to F: Abolished UDP-glucuronic acid decarboxylase activity.
K235 (= K150) binding NAD(+)
R236 (= R151) mutation to H: Strongly reduced UDP-glucuronic acid decarboxylase activity, caused by a local unfolding of the active site that allows for a rotation of the dimer interface, leading to the formation of a homohexamer.
Y245 (= Y160) binding UDP-alpha-D-glucuronate
Q248 (≠ F163) binding UDP-alpha-D-glucuronate
E249 (≠ H164) binding UDP-alpha-D-glucuronate
T261 (= T176) binding NAD(+)
H267 (≠ R182) binding NAD(+)
R272 (= R187) binding NAD(+)
R361 (= R276) mutation to Q: Strongly reduced UDP-glucuronic acid decarboxylase activity.

Sites not aligning to the query:

1 modified: N-acetylmethionine

Q6GMI9 UDP-glucuronic acid decarboxylase 1; UDP-glucuronate decarboxylase 1; UXS-1; EC 4.1.1.35 from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
52% identity, 93% coverage: 2:306/328 of query aligns to 87:389/418 of Q6GMI9

query
sites
Q6GMI9

K

K

K

R

T

I

L

L

I

I

T

T

G

G

A

G

A

A

G

G

F

F

L

V

G

G

S

S

H

H

L

L

C

T

D

D

R

K

F

L

L

M

A

M

E

D

G

G

H

H

Y

E

V

V

I

T

G

V

M

V

D

D

N

N

L

F

I

F

T

T

G

G

D

R

L

K

K

R

N

N

I

V

E

E

H

H

L

W

F

I

G

G

H

H

K

E

N

N

F

F

E

E

F

L

A

I

H

N

H

H

D

D

V

V

T

V

K

E

F

P

V

L

H

Y

V

I

P

-

G

-

S

E

L

V

D

D

Y

Q

I

I

L

Y

H

H

F

L

A

A

S

S

P

P

A

A

S

S

P

P

I

P

D

N

Y

Y

L

M

K

Y

I

N

P

P

I

I

Q

K

T

T

L

L

K

K

V

T

G

N

A

T

L

I

G

G

T

T

H

L

N

N

L

M

L

L

G

G

L

L

A

A

K

K

D

R

K

V

G

G

A

A

R

R

F

L

M

L

I

L

A

A

S

S

T

T

S

S

E

E

I

V

Y

Y

G

G

D

D

P

P

L

E

V

V

H

H

P

P

Q

Q

T

N

E

E

E

D

Y

Y

Y

W

G

G

N

H

V

V

N

N

T

P

I

I

G

G

P

P

R

R

G

A

V

C

Y

Y

D

D

E

E

A

G

K

K

R
|
R

F

V

Q

A

E

E

S

T

I

M

T

C

M

Y

A

A

Y

Y

H

M

T

K

F

Q

H

E

G

G

L

V

D

E

T

V

R

R

I

V

V

A

R

R

I

I

F

F

N

N

T

T

Y

F

G

G

P

S

R

R

M

M

R

H

L

M

N

N

D

D

G

G

R

R

V

V

I

V

P

S

A

N

F

F

M

I

G

L

Q

Q

A

A

L

L

R

Q

G

G

E

E

D

A

L

L

T

T

V

V

F

Y

G

G

D

S

G

G

S

S

Q

Q

T

T

R

R

S

A

F

F

C

Q

F

Y

V

V

D

S

D

D

E

L

I

V

E

N

G

G

I

L

Y

V

R

S

L

L

L

M

M

N

S

S

D

N

Y

I

N

S

L

S

P

P

M

V

N

N

I

L

G

G

N

N

P

P

H

E

E

E

I

H

T

T

I

I

K

L

D

E

F

F

A

A

E

Q

E

L

I

I

I

K

K

S

L

L

T

V

G

A

T

S

D

R

Q

S

K

H

I

I

I

Q

Y

F

K

L

P

P

L

E

P

A

K

Q

D

D

P

P

M

Q

Q

R

R

R

Q

R

P

P

D

D

I

I

T

R

K

K

A

A

R

K

E

L

I

L

L

L

G

G

W

W

E

E

P

P

K

V

V

V

G

P

R

L

E

E

G

G

M

L

R

N

K

K

T

T

F

I

E

Q

F

Y

F

F

R234 (= R151) mutation to H: In Man o' war (mow) mutant; characterized by severe craniofacial defects. Fishes show defective organization and morphogenesis of chondrocytes, perichondrium and bone.

2b69A Crystal structure of human udp-glucoronic acid decarboxylase
52% identity, 93% coverage: 2:307/328 of query aligns to 2:305/312 of 2b69A

query
sites
2b69A

K

K

K

R

T

I

L

L

I

I

T

T

G
|
G

A

G

A

A

G
|
G

F
|
F

L
x
V

G

G

S

S

H

H

L

L

C

T

D

D

R

K

F

L

L

M

A

M

E

D

G

G

H

H

Y

E

V

V

I

T

G

V

M

V

D
|
D

N
|
N

L

F

I

F

T
|
T

G
|
G

D

R

L

K

K

R

N

N

I

V

E

E

H

H

L

W

F

I

G

G

H

H

K

E

N

N

F

F

E

E

F

L

A

I

H

N

H

H

D
|
D

V
|
V

T

V

K

E

F
x
P

V
x
L

H
x
Y

V

I

P

-

G

-

S

E

L

V

D

D

Y

Q

I

I

L

Y

H

H

F
x
L

A
|
A

S
|
S

P

P

A
|
A

S

S

P
|
P

I

P

D

N

Y

Y

L

M

K

Y

I

N

P

P

I

I

Q

K

T

T

L

L

K

K

V
x
T

G

N

A

T

L

I

G

G

T

T

H

L

N
|
N

L

M

L

L

G
|
G

L
|
L

A

A

K
|
K

D
x
R

K

V

G

G

A

A

R

R

F

L

M

L

I

L

A

A

S

S

T
|
T

S
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S

E
|
E

I

V

Y

Y

G

G

D

D

P

P

L

E

V

V

H

H

P

P

Q

Q

T

S

E

E

E

D

Y

Y

Y

W

G

G

N

H

V

V

N

N

T

P

I

I

G

G

P

P

R

R

G

A

V

C

Y
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Y

D

D

E

E

A

G

K
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K

R

R

F

V

Q

A

E

E

S

T

I

M

T

C

M

Y

A

A

Y
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Y

H

M

T

K

F

Q

H

E

G

G

L

V

D

E

T

V

R

R

I

V

V

A

R

R

I
|
I

F

F

N
|
N

T

T

Y

F

G

G

P

P

R

R

M

M

R

H

L

M

N

N

D

D

G

G

R
|
R

V
|
V

I

V

P

S

A
x
N

F

F

M

I

G

L

Q

Q

A

A

L

L

R

Q

G

G

E

E

D

P

L

L

T
|
T

V

V

F
x
Y

G

G

D

S

G

G

S

S

Q
|
Q

T

T

R
|
R

S

A

F

F

C

Q

F

Y

V

V

D

S

D

D

E

L

I

V

E

N

G

G

I

L

Y

V

R

A

L

L

L

M

M

N

S

S

D

N

Y

V

N

S

L

S

P

P

M

V

N

N

I

L

G

G

N

N

P

P

H

E

E

E

I

H

T

T

I
|
I

K

L

D

E

F

F

A

A

E

Q

E

L

I

I

I

K

K

N

L

L

T

V

G

G

T

S

D

G

Q

S

K

E

I

I

I

Q

Y

F

K

L

P

S

L

E

P

A

K

Q

D

D

D
|
D

P

P

M

Q

Q

K

R

R

Q

K

P

P

D

D

I

I

T

K

K

K

A

A

R

K

E

L

I

M

L

L

G

G

W

W

E

E

P

P

K

V

V

V

G

P

R

L

E

E

G

G

M

L

R

N

K

K

T

A

F

I

E

H

F

Y

F

F

K

R

active site: T115 (= T117), S116 (= S118), E117 (= E119), Y144 (= Y146), K148 (= K150), R185 (= R187)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), F12 (= F12), V13 (≠ L13), D32 (= D32), N33 (= N33), T36 (= T36), G37 (= G37), D57 (= D57), V58 (= V58), L72 (≠ F74), A73 (= A75), S74 (= S76), A76 (= A78), T91 (≠ V93), T115 (= T117), Y144 (= Y146), K148 (= K150), I171 (= I173), N173 (= N175), R185 (= R187)
binding uridine-5'-diphosphate: P61 (≠ F61), L62 (≠ V62), Y63 (≠ H63), P78 (= P80), N98 (= N100), G101 (= G103), L102 (= L104), K104 (= K106), R105 (≠ D107), Y158 (= Y160), N173 (= N175), R185 (= R187), V186 (= V188), N189 (≠ A191), T201 (= T203), Y203 (≠ F205), Q208 (= Q210), R210 (= R212), I244 (= I246), D270 (= D272)

4lk3B Crystal structure of human udp-xylose synthase r236a substitution (see paper)
44% identity, 93% coverage: 2:307/328 of query aligns to 2:267/274 of 4lk3B

query
sites
4lk3B

K

K

K

R

T

I

L

L

I

I

T

T

G
|
G

A

G

A

A

G
|
G

F
|
F

L
x
V

G

G

S

S

H

H

L

L

C

T

D

D

R

K

F

L

L

M

A

M

E

D

G

G

H

H

Y

E

V

V

I

T

G

V

M

V

D
|
D

N
|
N

L

F

I

F

T
|
T

G
|
G

D

R

L

K

K

R

N

N

I

V

E

E

H

H

L

W

F

I

G

G

H

H

K

E

N

N

F

F

E

E

F

L

A

I

H

N

H

H

D
|
D

V
|
V

T

V

K

E

F
x
P

V
x
L

H
x
Y

V

I

P

-

G

-

S

E

L

V

D

D

Y

Q

I

I

L

Y

H

H

F
x
L

A
|
A

S
|
S

P
|
P

A

A

S

S

P

P

I

-

D

-

Y

-

L

M

K

Y

I

N

P

P

I
|
I

Q

K

T

T

L

L

K
|
K

V
x
T

G

N

A

T

L

I

G

G

T

T

H

L

N
|
N

L

M

L

L

G
|
G

L
|
L

A

A

K
|
K

D

R

K

V

G

G

A

A

R

R

F

L

M

L

I

L

A
|
A

S

S

T
|
T

S
|
S

E
|
E

I

V

Y

Y

G

-

D

-

P

-

L

-

V

-

H

-

P

-

Q

-

T

-

E

-

Y

-

G

-

N

-

V

-

N

-

T

-

I

-

G

-

P

-

R

-

G

-

V

-

Y

-

D

-

E

E

A

G

K
|
K

R

A

F

V

Q

A

E

E

S

T

I

M

T

C

M

Y

A

A

Y
|
Y

H

M

T

K

F

Q

H
x
E

G

G

L

V

D

E

T

V

R

R

I

V

V

A

R

R

I
|
I

F

F

N

N

T

T

Y

F

G

G

P

P

R

R

M

M

R
x
H

L

M

N

N

D

D

G

G

R
|
R

V
|
V

I

V

P

S

A
x
N

F

F

M

I

G

L

Q

Q

A

A

L

L

R

Q

G

G

E

E

D

P

L

L

T
|
T

V

V

F
x
Y

G

G

D

S

G

G

S

S

Q
|
Q

T

T

R
|
R

S

A

F

F

C

Q

F

Y

V

V

D

S

D

D

E

L

I

V

E

N

G

G

I

L

Y

V

R

A

L

L

L

M

M

N

S

S

D

N

Y

V

N

S

L

S

P

P

M

V

N

N

I

L

G

G

N

N

P

P

H

E

E

E

I

H

T

T

I
|
I

K

L

D

E

F

F

A

A

E

Q

E

L

I

I

I

K

K

N

L

L

T

V

G

G

T

S

D

G

Q

S

K

E

I

I

I

Q

Y

F

K

-

P

-

L

-

P

-

K

-

D

-

P

-

M

-

Q

L

R

R

Q

K

P

P

D

D

I

I

T

K

K

K

A

A

R

K

E

L

I

M

L

L

G

G

W

W

E

E

P

P

K

V

V

V

G

P

R

L

E

E

G

G

M

L

R

N

K

K

T

A

F

I

E

H

F

Y

F

F

K

R

active site: T112 (= T117), S113 (= S118), E114 (= E119), K119 (= K150), R156 (= R187)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), F12 (= F12), V13 (≠ L13), D32 (= D32), N33 (= N33), T36 (= T36), G37 (= G37), D57 (= D57), V58 (= V58), L72 (≠ F74), A73 (= A75), S74 (= S76), P75 (= P77), T88 (≠ V93), A110 (= A115), T112 (= T117), K119 (= K150), I142 (= I173), H151 (≠ R182)
binding uridine-5'-diphosphate: R156 (= R187), V157 (= V188), N160 (≠ A191), T172 (= T203), Y174 (≠ F205), Q179 (= Q210), R181 (= R212), I215 (= I246)
binding uridine-5'-diphosphate-glucuronic acid: P61 (≠ F61), L62 (≠ V62), Y63 (≠ H63), I83 (= I88), K87 (= K92), N95 (= N100), G98 (= G103), L99 (= L104), K101 (= K106), Y129 (= Y160), E133 (≠ H164)

4lk3C Crystal structure of human udp-xylose synthase r236a substitution (see paper)
43% identity, 93% coverage: 2:307/328 of query aligns to 2:264/271 of 4lk3C

query
sites
4lk3C

K

K

K

R

T

I

L

L

I

I

T

T

G
|
G

A

G

A

A

G
|
G

F
|
F

L
x
V

G

G

S

S

H

H

L

L

C

T

D

D

R

K

F

L

L

M

A

M

E

D

G

G

H

H

Y

E

V

V

I

T

G

V

M

V

D
|
D

N
|
N

L

F

I

F

T
|
T

G
|
G

D

R

L

K

K

R

N

N

I

V

E

E

H

H

L

W

F

I

G

G

H

H

K

E

N

N

F

F

E

E

F

L

A

I

H

N

H

H

D
|
D

V
|
V

T

V

K

E

F
x
P

V
x
L

H
x
Y

V

I

P

-

G

-

S

E

L

V

D

D

Y

Q

I

I

L

Y

H

H

F
x
L

A
|
A

S
|
S

P
|
P

A

A

S

S

P

-

I

-

D

-

Y

-

L

-

K

Y

I

N

P

P

I

I

Q

K

T

T

L

L

K

K

V
x
T

G

N

A

T

L

I

G

G

T

T

H

L

N
|
N

L

M

L

L

G
|
G

L
|
L

A

A

K
|
K

D
x
R

K

V

G

G

A

A

R

R

F

L

M

L

I

L

A

A

S

S

T
|
T

S
|
S

E
|
E

I

V

Y

Y

G

-

D

-

P

-

L

-

V

-

H

-

P

-

Q

-

T

-

E

-

Y

-

G

-

N

-

V

-

N

-

T

-

I

-

G

-

P

-

R

-

G

-

V

-

Y

-

D

-

E

E

A

G

K
|
K

R

A

F

V

Q

A

E

E

S

T

I

M

T

C

M

Y

A

A

Y
|
Y

H

M

T

K

F

Q

H
x
E

G

G

L

V

D

E

T

V

R

R

I

V

V

A

R

R

I
|
I

F

F

N

N

T

T

Y

F

G

G

P

P

R

R

M

M

R
x
H

L

M

N

N

D

D

G

G

R
|
R

V
|
V

I

V

P

S

A

N

F

F

M

I

G

L

Q

Q

A

A

L

L

R

Q

G

G

E

E

D

P

L

L

T

T

V

V

F

Y

G

G

D

S

G

G

S

S

Q

Q

T

T

R

R

S

A

F

F

C

Q

F

Y

V

V

D

S

D

D

E

L

I

V

E

N

G

G

I

L

Y

V

R

A

L

L

L

M

M

N

S

S

D

N

Y

V

N

S

L

S

P

P

M

V

N

N

I

L

G

G

N

N

P

P

H

E

E

E

I

H

T

T

I

I

K

L

D

E

F

F

A

A

E

Q

E

L

I

I

I

K

K

N

L

L

T

V

G

G

T

S

D

G

Q

S

K

E

I

I

I

Q

Y

F

K

-

P

-

L

-

P

-

K

-

D

-

P

-

M

-

Q

-

R

L

Q

K

P

P

D

D

I

I

T

K

K

K

A

A

R

K

E

L

I

M

L

L

G

G

W

W

E

E

P

P

K

V

V

V

G

P

R

L

E

E

G

G

M

L

R

N

K

K

T

A

F

I

E

H

F

Y

F

F

K

R

active site: T110 (= T117), S111 (= S118), E112 (= E119), K117 (= K150), R154 (= R187)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), F12 (= F12), V13 (≠ L13), D32 (= D32), N33 (= N33), T36 (= T36), G37 (= G37), D57 (= D57), V58 (= V58), L72 (≠ F74), A73 (= A75), S74 (= S76), P75 (= P77), T86 (≠ V93), K117 (= K150), I140 (= I173), H149 (≠ R182)
binding pyrophosphate 2-: R154 (= R187), V155 (= V188)
binding uridine-5'-diphosphate-glucuronic acid: P61 (≠ F61), L62 (≠ V62), Y63 (≠ H63), N93 (= N100), G96 (= G103), L97 (= L104), K99 (= K106), R100 (≠ D107), Y127 (= Y160), E131 (≠ H164)

8vr2B Crystal structure of the pcryo_0617 oxidoreductase/decarboxylase from psychrobacter cryohalolentis k5 in the presence of NAD and udp
33% identity, 94% coverage: 2:308/328 of query aligns to 8:313/321 of 8vr2B

query
sites
8vr2B

K

K

T

I

L

F

I

I

T

T

G
|
G

A

G

A

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

T

L

L

C

I

D

G

R

R

F

L

L

I

A

-

E

E

G

N

H

N

Y

E

V

M

I

I

G

V

M

Y

D
|
D

N
|
N

L

L

I
x
E

T
x
R

G

N

D

T

L

L

K

K

N

S

I

-

E

Q

H

P

L

F

F

A

G

N

H

H

K

K

N

N

F

L

E

T

F

L

A

I

H

Q

H

G

D
x
N

V
|
V

T

L

-

D

-

Q

-

E

K

K

F

I

V

I

H

E

V

A

P

A

G

K

S

G

L

S

D

E

Y

I

I

F

L

I

H

H

F
x
A

A
|
A

S
x
A

P

I

A

A

S

G

P

I

I

D

D

N

Y

T

L

V

K

K

I

S

P

P

I

V

Q

R

T

T

L

M

K

T

V

V

G

N

A

M

L

I

G

G

T

T

H

A

N

N

L

A

L

L

G

E

L

A

A

A

K

H

D

Q

K

A

G

G

A

T

-

V

-

Q

R

R

F

F

M

L

I

E

A
x
F

S

S

T

T

S

S

E

E

I

V

Y

F

G

G

D

S

P

R

L

A

V

Y

H

R

P

V

Q

D

T

E

E

T

E

G

Y

A

Y

V

G

G

N

E

V

-

N

-

T

-

I

-

G

-

P

A

R
|
R

G

W

V

T

Y

Y

D

A

E

V

A

S

K
|
K

R

L

F

A

Q

G

E

E

S

H

I

L

T

T

M

H

A

A

Y

Y

H

N

T

R

F

E

H

H

G

G

L

L

D

P

T

T

R

V

I

T

V

F

R

R

I
x
P

F

F

N
|
N

T

V

Y

Y

G

G

P

P

R

G

M

-

R

Q

L

I

N

G

D

E

G
|
G

R

-

V
x
A

I

I

P

S

A

I

F
x
M

M

I

G

R

Q

K

A

A

L

L

R

N

G

N

E

E

D

D

L

I

T
x
Y

V
x
I

F
|
F

G

G

D

D

G

G

S

S

Q

Q

T

I

R
|
R

S

A

F

W

C

C

F

Y

V

V

D

D

E

M

I

I

E

D

G

A

I

L

Y

M

R

K

L

A

L

L

M

S

S

V

D

P

Y

Q

N

A

L

I

-

G

-

E

P

S

M

F

N

N

I

I

G

G

N

N

P

A

H

R

E

A

I

I

-

T

T

T

I
|
I

K

Y

D

G

F

L

A

A

E

Q

E

T

I

I

I

C

K

R

L

V

T

L

G

N

T

S

D

K

Q

S

K

E

I

I

Y

F

K

R

P

E

L

A

P

L

K

S

D

A

D

D

P

I

M

E

Q

L

R

R

Q

I

P

P

D

N

I

V

T

D

K

K

A

S

R

E

E

E

I

L

L

L

G

G

W

F

E

K

P

A

K

Q

V

V

G

D

R

L

E

E

G

G

M

L

R

I

K

R

T

T

F

A

E

D

F

W

F

L

K

S

T

A

binding nicotinamide-adenine-dinucleotide: G14 (= G8), G17 (= G11), F18 (= F12), I19 (≠ L13), D37 (= D32), N38 (= N33), E40 (≠ I35), R41 (≠ T36), N61 (≠ D57), V62 (= V58), A81 (≠ F74), A82 (= A75), A83 (≠ S76), F124 (≠ A115), K154 (= K150), P177 (≠ I173), N179 (= N175)
binding uridine-5'-diphosphate: R147 (= R143), G189 (= G186), A190 (≠ V188), M194 (≠ F192), Y205 (≠ T203), I206 (≠ V204), F207 (= F205), R214 (= R212), I251 (= I246)

4zrnA Crystal structure of udp-glucose 4-epimerase (tm0509) with udp-glucose from hyperthermophilic eubacterium thermotoga maritima (see paper)
33% identity, 92% coverage: 5:307/328 of query aligns to 4:305/309 of 4zrnA

query
sites
4zrnA

L

L

I

V

T

T

G
|
G

A

G

A

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

H

L

V

C

V

D

D

R

K

F

L

L

I

A

E

E

N

G

G

H

Y

Y

G

V

V

I

I

G

V

M

V

D
|
D

N
|
N

L

L

I
x
S

T
x
S

G
|
G

D

K

L

V

K

E

N

N

I

L

E

N

H

R

-

N

-

A

L

L

F

F

G

Y

H

E

K

Q

N
x
S

F
x
I

E

E

F

D

A

E

H

E

H

M

D

M

V

E

T

R

K

I

F

F

-

S

V

L

H

H

V

R

P

P

G

-

S

-

L

-

D

E

Y

Y

I

V

L

F

H

H

F
x
L

A
|
A

S
x
A

P

Q

A

A

S

S

P

V

I

A

D

I

Y

S

L

V

K

R

I

E

P

P

I

A

Q

R

T

D

L

A

K

K

V
x
T

G

N

A

I

L

I

G

G

T

S

H

L

N

V

L

L

G

E

L

K

A

S

K

I

D

K

K

Y

G

G

A

V

R

K

-

K

F

F

M

I

I

F

A
x
S

S
|
S

T
|
T

S

G

-
x
G

E
x
A

I

I

Y

Y

G

G

D

E

P

N

L

V

V

K

-

V

-

F

-

P

H

T

P

P

Q

E

T

T

E

E

E

-

Y

-

G

-

N

I

V

P

N

H

T

P

I

I

G

S

P

P

R
x
Y

G

G

V

I

Y

-

D

-

E

-

A

A

K
|
K

R

Y

F

S

Q

T

E

E

S

M

I

Y

T

L

M

E

A

F

Y

F

H

A

T

R

F

E

H

Y

G

G

L

L

D

K

T

Y

R

T

I

V

V

L

R

R

I
x
Y

F

A

N
|
N

T
x
V

Y

Y

G

G

P

P

R

R

M

Q

R

D

-

P

L
x
Y

N

G

D

E

G

A

R
x
G

V
|
V

I

V

P

A

A

I

F

F

M

T

G

E

Q

R

A

M

L

L

R

R

G

G

E

E

D

E

L

V

T
x
H

V

I

F
|
F

G

G

D

D

G

G

S

E

Q
x
Y

T

V

R
|
R

S

D

F

Y

C

V

F

Y

V

V

D

D

E

V

I

V

E

R

G

A

I

N

Y

L

R

L

L

A

L

M

M

E

S

K

D

G

Y

D

N

N

L

E

P

V

M

F

N

N

I

I

G

G

N

T

P

G

H

R

E

G

I

T

T

T

I
x
V

K

N

D

Q

F

L

A

F

E

K

E

L

I

L

I

K

K

E

L

I

T

T

G

G

T

Y

D

D

Q

K

K

E

I

P

I

V

Y

Y

K

K

P

P

L

P

P
x
R

K

K

D

G

D
|
D

P

V

M

R

Q

K

R

S

Q

I

P

L

D

D

I

Y

T

T

K

K

A

A

R

K

E

E

I

K

L

L

G

G

W

W

E

E

P

P

K

K

V

V

G

S

R

L

E

E

G

G

M

L

R

K

K

L

T

T

F

V

E

E

F

Y

F

F

K

R

active site: T117 (= T117), G119 (vs. gap), A120 (≠ E119), Y143 (≠ R143), K147 (= K150), Y181 (≠ L183), G185 (≠ R187)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (≠ L13), D31 (= D32), N32 (= N33), S34 (≠ I35), S35 (≠ T36), G36 (= G37), S51 (≠ N50), I52 (≠ F51), L73 (≠ F74), A74 (= A75), A75 (≠ S76), T92 (≠ V93), S115 (≠ A115), S116 (= S116), Y143 (≠ R143), K147 (= K150), Y170 (≠ I173), V173 (≠ T176)
binding uridine-5'-diphosphate-glucose: T117 (= T117), G119 (vs. gap), A120 (≠ E119), Y143 (≠ R143), N172 (= N175), G185 (≠ R187), V186 (= V188), H201 (≠ T203), F203 (= F205), Y208 (≠ Q210), R210 (= R212), V244 (≠ I246), R267 (≠ P269), D270 (= D272)

8rdhA Crystal structure of udp-galactose 4-epimerase from pyrococcus horikoshii with bound NAD and gdp-l-fucose
31% identity, 91% coverage: 3:302/328 of query aligns to 2:309/319 of 8rdhA

query
sites
8rdhA

K

R

T

V

L

L

I

V

T

T

G
|
G

A

G

A

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

H

L

L

C

V

D

D

R

R

F

L

L

M

A

E

E

E

G

G

H

Y

Y

K

V

V

I

R

G

V

M

L

D
|
D

N
x
D

L

L

I
x
S

T
x
A

G
|
G

D

S

L

L

K

K

N

N

I

I

E

E

H

G

L

W

F

L

G

G

H

N

K

E

N

N

F

F

E

E

F

F

A

I

H

K

H

G

D

D

V
x
M

T

R

K

D

F

V

V

E

H

I

V

V

P

S

G

K

S

A

-

V

-

K

-

D

L

V

D

D

Y

A

I

V

L

F

H

H

F
x
L

A
|
A

S
x
A

P

N

A
x
P

S

E

P

V

I
x
R

D

I

Y

G

L

S

K

Q

I

S

P

P

I

E

Q

L

T

L

L

Y

K

E

V

T

G

N

A

V

L

L

G

I

T

T

H

Y

N

N

L

L

G

N

L

A

A

V

K

R

D

N

K

S

G

G

A

V

R

K

F

Y

M

L

I

V

-

F

A
x
T

S

S

T
x
S

S

S

E

T

I

V

Y

Y

G

G

D

D

P

A

L

K

V

V

H

I

P

P

Q

T

T

P

E

E

E

D

Y

Y

Y

A

G

-

N

-

V

-

N

-

T

P

I

L

G

E

P

P

R

I

G

S

V

V

Y
|
Y

D

G

E

A

A

A

K
|
K

R

L

F

A

Q

A

E

E

S

A

I

L

T

I

M

S

A

G

Y

Y

-

A

H

H

T

T

F

F

H

D

G

-

L

F

D

R

T

A

R

L

I

I

V

I

R

R

I
x
L

F
x
A

N
|
N

T
x
I

Y

I

G

G

P

K

R

R

M

S

R

-

L

-

N

N

D
x
H

G
|
G

R
x
V

V

I

I

Y

P
x
D

A
x
F

F

I

M

N

G
x
K

Q

L

A

K

L

A

R

N

G

P

E

N

D

E

L

L

T

E

V

I

F
x
L

G

G

D

D

G

G

S

T

Q
|
Q

T

R

R
x
K

S

S

F

Y

C

L

F

H

V

I

D

S

D

D

E

T

I

I

E

D

G

G

I

I

Y

M

R

K

L

L

L

F

M

E

S

H

D

F

Y

L

N

N

L

G

P

E

-

E

-

R

-

V

-

D

-

F

M

Y

N

N

I

L

G

G

N

N

P

E

H

D

E

W

I

I

T

T

I
x
V

K

K

D

E

F

I

A

A

E

E

E

I

I

V

I

S

K

E

L

E

T

M

G

N

T

L

D

N

Q

P

K

R

I

F

I

K

Y

F

K

T

P

G

L

G

P

V

-

D

-

G

-

R

-

G

-
x
W

K

K

D

G

D
|
D

P

V

M

K

Q

L

R

M

Q

L

P

L

D

S

I

I

T

E

K

K

A

A

R

K

E

R

I

T

L

-

G

G

W

W

E

K

P

P

K

R

V

M

G

N

R

S

E

Y

E

E

G

A

M

V

R

R

K

K

T

T

binding guanosine-5'-diphosphate-beta-l-fucopyranose: R83 (≠ I81), S120 (≠ T117), Y145 (= Y146), N174 (= N175), G183 (= G186), V184 (≠ R187), D187 (≠ P190), F188 (≠ A191), K191 (≠ G194), L202 (≠ F205), Q207 (= Q210), K209 (≠ R212), V248 (≠ I246), W277 (vs. gap), D280 (= D272)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (≠ L13), D31 (= D32), D32 (≠ N33), S34 (≠ I35), A35 (≠ T36), G36 (= G37), M57 (≠ V58), L76 (≠ F74), A77 (= A75), A78 (≠ S76), P80 (≠ A78), T118 (≠ A115), Y145 (= Y146), K149 (= K150), L172 (≠ I173), A173 (≠ F174), N174 (= N175), I175 (≠ T176), H182 (≠ D185)

Q9LPG6 Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM2; NDP-rhamnose synthase; Protein MUCILAGE-MODIFIED 4; Protein RHAMNOSE BIOSYNTHESIS 2; Rhamnose biosynthetic enzyme 2; AtRHM2; UDP-L-rhamnose synthase MUM4; EC 4.2.1.76; EC 1.1.1.-; EC 5.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
29% identity, 93% coverage: 2:306/328 of query aligns to 9:321/667 of Q9LPG6

query
sites
Q9LPG6

K

K

K

N

T

I

L

L

I

I

T

T

G

G

A

A

G
|
G

F

F

L

I

G

A

S

S

H

H

L

V

C

A

D

N

R

R

F

L

L

I

A

R

E

N

-

Y

-

P

G

D

H

Y

Y
x
K

V

I

I

V

G

V

M

L

D

D

N

K

L

L

-

D

I

Y

T

C

G

S

D

D

L

L

K

K

N

N

I

L

E

D

H

P

L

S

F

F

G

S

H

S

K

P

N

N

F

F

E

K

F

F

-

V

-

K

-

G

-

D

-

I

A

A

H

S

H

D

D

D

V

L

T

V

K

N

F

Y

V

L

H

L

V

I

P

T

G

E

S

N

L

I

D

D

Y

T

I

I

L

M

H

H

F

F

A

A

S

A

P

Q

A

T

S

H

P

V

I
x
D

D

N

Y

S

L

F

K

G

I

N

P

S

I

F

Q

E

T

F

L

T

K

K

V

N

G

N

A

I

L

Y

G

G

T

T

H

H

N

V

L

L

G

E

L

A

A

C

K

K

D

V

K

T

G

G

A

Q

-

I

-

R

R

R

F

F

M

I

I

H

A

V

S

S

T

T

S

D

E

E

I

V

Y

Y

G

G

D

E

P

T

L

-

V

-

H

-

P

-

Q

D

T

E

E

D

E

A

Y

A

Y

V

G

G

N

N

-

H

-

E

V

A

N

S

T

Q

I

L

G

L

P

P

R

T

G

N

V

P

Y

Y

D

S

E

A

A

T

K
|
K

R

A

F

G

Q

A

E

E

S

M

I

L

T

V

M

M

A

A

Y

Y

H

G

T

R

F

S

H

Y

G

G

L

L

D

P

T

V

R

I

I

T

V

T

R

R

I

G

F

N

N

N

T

V

Y

Y

G
|
G

P

P

R

N

M

Q

R

F

L

P

N

E

D

-

G

-

R

K

V

M

I

I

P

P

A

K

F

F

M

I

G

L

Q

L

A

A

L

M

R

S

G

G

E

K

D

P

L

L

T

P

V

I

F

H

G

G

D

D

G

G

S

S

Q

N

T

V

R

R

S

S

F

Y

C

L

F

Y

V

C

D

E

D

D

E

V

I

A

E

E

G

A

I

F

Y

E

R

V

L

V

L

L

-

H

M

K

S

G

D

E

Y

I

N

G

L

H

P

V

M

Y

N

N

I

V

G

G

N

T

P

K

H

R

E

E

I

R

T

R

I

V

K

I

D

D

F

V

A

A

E

R

E

D

I

I

I

C

K

K

L

L

T

F

G

G

T

K

D

D

Q

P

K

E

I

S

Y

I

K

Q

P

-

L

F

P

V

K

E

D

N

D

R

P

P

M

F

Q

N

R

D

Q

Q

P

R

D

Y

I

F

T

L

K

D

A

D

R

Q

E

K

I

L

-

K

L

L

G

G

W

W

E

Q

P

E

K

R

V

T

G

N

R

W

E

E

E

D

G

G

M

L

R

K

K

K

T

T

F

M

E

D

F

W

F

Y

G18 (= G11) mutation to A: Abolishes dehydratase activity.
K36 (≠ Y27) mutation to A: Reduces dehydratase activity.
D96 (≠ I81) mutation to N: In mum4-1; no extruded mucilage and seed coat defects. Abolishes dehydratase activity.
K165 (= K150) mutation to A: Abolishes dehydratase activity.
G193 (= G178) mutation to R: In mum4-2; no extruded mucilage and seed coat defects. Abolishes dehydratase activity.

Sites not aligning to the query:

392 G→A: No effect on dehydratase activity.
413 K→A: No effect on dehydratase activity.
518 K→A: No effect on dehydratase activity.

6zllA Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp-galacturonic acid and NAD (see paper)
31% identity, 94% coverage: 3:311/328 of query aligns to 2:315/321 of 6zllA

query
sites
6zllA

K

K

T

I

L

L

I

V

T

T

G
|
G

A

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

H

L

L

C

C

D

Q

R

A

F

L

L

L

A

K

E

N

G

S

H

A

Y

Y

-

H

V

V

I

V

G

G

M

I

D
|
D

N
x
H

L
x
F

I
|
I

-

G

-

P

-

T

-

P

-

A

T

T

G

L

D
x
K

L

T

K

G

N

N

I

I

E

Q

H

S

L

L

F

E

G

L

H

N

K

S

N

R

F

F

E

Q

F

F

A

I

H

R

H

E

D
|
D

V
x
I

-

L

-

N

T

T

K

D

F

L

V

S

H

K

V

L

P

L

G

Q

S

D

L

I

D

D

Y

V

I

V

L

Y

H

H

F
x
L

A
|
A

S
x
A

-

I

P
|
P

A

G

S
x
V

P
x
R

I

T

D

S

Y

W

L

G

K

K

I

D

P

F

I

Q

Q

P

T

Y

L

V

K

T

V

N

G

N

A

I

L

M

G

V

T

T

H

Q

N

Q

L

L

G

E

L

A

A

C

K

K

D

H

-

I

K

K

G

L

A

D

R

K

F

F

M

I

I

H

A
x
I

S

S

T
|
T

S
|
S

E
x
S

I

V

Y

Y

G

G

D

-

P

-

L

-

V

-

H

-

P

-

Q

-

T

-

E

-

E

E

Y

K

Y

S

G

G

N

A

V

V

N

S

T

E

-

D

-

L

-

P

-

I

-

P

I

L

G

S

P

P

R
x
Y

G

G

V

V

Y

-

D

-

E

-

A

T

K
|
K

R

L

F

S

Q

G

E

E

S

H

I

L

T

C

M

H

A

V

Y

Y

H

H

T

K

F

N

H

F

G

H

L

I

D

P

T

I

R

V

I

I

V

L

R

R

I
x
Y

F

F

N
x
T

T
x
V

Y

Y

G

G

P

P

R

R

M

Q

R
|
R

L

P

N

D

D

-

G

-

R
x
M

V
x
A

I

F

P

H

A
x
R

F

L

M

I

G

K

Q

Q

A

M

L

L

R

E

G

D

E

K

D

P

L

L

T
|
T

V

I

F
|
F

G

G

D

D

G

G

S

T

Q
|
Q

T

T

R
|
R

S

D

F

F

C

T

F

Y

V

I

D

D

E

C

I

I

E

R

G

G

I

T

Y

V

R

A

L

A

L

L

M

E

S

T

D

K

Y

K

N

N

L

I

-

I

-

G

-

E

P

V

M

I

N

N

I

I

G

G

N

G

P

K

H

E

E

Q

I

A

T

S

I
|
I

K

L

D

D

F

I

A

I

E

S

E

M

I

L

I

E

K

K

L

I

T

S

G

G

-

K

T

S

D

A

Q

T

K

K

I

N

I

F

Y

L

K

K

P

S

L

V

P

P

K

-

D

G

D
x
E

P

P

M

K

Q

Q

R

T

Q

W

P

A

D

D

I

I

T

S

K

K

A

A

R

S

E

T

I

L

L

L

G

Q

W

Y

E

S

P

P

K

T

V

V

G

S

R

L

E

S

E

D

G

G

M

L

R

E

K

A

T

E

F

Y

E

D

F

Y

F

I

K

K

T

Q

L

L

T

Y

K

K

active site: T126 (= T117), S127 (= S118), S128 (≠ E119), Y149 (≠ R143), K153 (= K150)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (≠ L13), D32 (= D32), H33 (≠ N33), F34 (≠ L34), I35 (= I35), K43 (≠ D38), D62 (= D57), I63 (≠ V58), L81 (≠ F74), A82 (= A75), A83 (≠ S76), I124 (≠ A115), T126 (= T117), Y149 (≠ R143), K153 (= K150), Y176 (≠ I173), V179 (≠ T176), R185 (= R182), M188 (≠ R187)
binding (2S,3R,4S,5R,6R)-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4,5-trihydroxy-oxane-2-carboxylic acid: P85 (= P77), V87 (≠ S79), R88 (≠ P80), T126 (= T117), S127 (= S118), Y149 (≠ R143), T178 (≠ N175), R185 (= R182), A189 (≠ V188), R192 (≠ A191), T204 (= T203), F206 (= F205), Q211 (= Q210), R213 (= R212), I250 (= I246), E276 (≠ D272)

6zldA Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp-glucuronic acid and NAD (see paper)
31% identity, 94% coverage: 3:309/328 of query aligns to 2:313/314 of 6zldA

query
sites
6zldA

K

K

T

I

L

L

I

V

T

T

G
|
G

A

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

H

L

L

C

C

D

Q

R

A

F

L

L

L

A

K

E

N

G

S

H

A

Y

Y

-

H

V

V

I

V

G

G

M

I

D
|
D

N
x
H

L
x
F

I
|
I

-

G

-

P

-

T

-

P

-

A

T

T

G

L

D
x
K

L

T

K

G

N

N

I

I

E

Q

H

S

L

L

F

E

G

L

H

N

K

S

N

R

F

F

E

Q

F

F

A

I

H

R

H

E

D
|
D

V
x
I

-

L

-

N

T

T

K

D

F

L

V

S

H

K

V

L

P

L

G

Q

S

D

L

I

D

D

Y

V

I

V

L

Y

H

H

F
x
L

A
|
A

S
x
A

-

I

P
|
P

A

G

S

V

P
x
R

I

T

D

S

Y

W

L

G

K

K

I

D

P

F

I

Q

Q

P

T

Y

L

V

K

T

V

N

G

N

A

I

L

M

G

V

T

T

H

Q

N

Q

L

L

G

E

L

A

A

C

K

K

D

H

-

I

K

K

G

L

A

D

R

K

F

F

M

I

I

H

A
x
I

S

S

T
|
T

S
|
S

E
x
S

I

V

Y

Y

G

G

D

-

P

-

L

-

V

-

H

-

P

-

Q

-

T

-

E

-

E

E

Y

K

Y

S

G

G

N

A

V

V

N

S

T

E

-

D

-

L

-

P

-

I

-

P

I

L

G

S

P

P

R
x
Y

G

G

V

V

Y

-

D

-

E

-

A

T

K
|
K

R

L

F

S

Q

G

E

E

S

H

I

L

T

C

M

H

A

V

Y

Y

H

H

T

K

F

N

H

F

G

H

L

I

D

P

T

I

R

V

I

I

V

L

R

R

I
x
Y

F
|
F

N
x
T

T

V

Y

Y

G

G

P

P

R

R

M

Q

R
|
R

L

P

N

D

D

-

G

-

R
x
M

V
x
A

I

F

P

H

A
x
R

F

L

M

I

G

K

Q

Q

A

M

L

L

R

E

G

D

E

K

D

P

L

L

T
|
T

V

I

F
|
F

G

G

D

D

G

G

S

T

Q
|
Q

T

T

R
|
R

S

D

F

F

C

T

F

Y

V

I

D

D

E

C

I

I

E

R

G

G

I

T

Y

V

R

A

L

A

L

L

M

E

S

T

D

K

Y

K

N

N

L

I

-

I

-

G

-

E

P

V

M

I

N

N

I

I

G

G

N

G

P

K

H

E

E

Q

I

A

T

S

I
|
I

K

L

D

D

F

I

A

I

E

S

E

M

I

L

I

E

K

K

L

I

T

S

G

G

-

K

T

S

D

A

Q

T

K

K

I

N

I

F

Y

L

K

K

P

S

L

V

P

P

K

-

D

G

D
x
E

P

P

M

K

Q

Q

R

T

Q

W

P

A

D

D

I

I

T

S

K

K

A

A

R

S

E

T

I

L

L

L

G

Q

W

Y

E

S

P

P

K

T

V

V

G

S

R

L

E

S

E

D

G

G

M

L

R

E

K

A

T

E

F

Y

E

D

F

Y

F

I

K

K

T

Q

L

L

active site: T126 (= T117), S127 (= S118), S128 (≠ E119), Y149 (≠ R143), K153 (= K150)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (≠ L13), D32 (= D32), H33 (≠ N33), F34 (≠ L34), I35 (= I35), K43 (≠ D38), D62 (= D57), I63 (≠ V58), L81 (≠ F74), A82 (= A75), A83 (≠ S76), I124 (≠ A115), T126 (= T117), K153 (= K150), Y176 (≠ I173), T178 (≠ N175), R185 (= R182), M188 (≠ R187)
binding uridine-5'-diphosphate-glucuronic acid: P85 (= P77), R88 (≠ P80), T126 (= T117), S127 (= S118), S128 (≠ E119), Y149 (≠ R143), F177 (= F174), T178 (≠ N175), R185 (= R182), M188 (≠ R187), A189 (≠ V188), R192 (≠ A191), T204 (= T203), F206 (= F205), Q211 (= Q210), R213 (= R212), I250 (= I246), E276 (≠ D272)

6zl6A Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp and NAD (see paper)
31% identity, 94% coverage: 3:309/328 of query aligns to 2:313/314 of 6zl6A

query
sites
6zl6A

K

K

T

I

L

L

I

V

T

T

G
|
G

A

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

H

L

L

C

C

D

Q

R

A

F

L

L

L

A

K

E

N

G

S

H

A

Y

Y

-

H

V

V

I

V

G

G

M

I

D
|
D

N
x
H

L
x
F

I
|
I

-

G

-

P

-

T

-

P

-

A

T

T

G

L

D
x
K

L

T

K

G

N

N

I

I

E

Q

H

S

L

L

F

E

G

L

H

N

K

S

N

R

F

F

E

Q

F

F

A

I

H

R

H

E

D
|
D

V
x
I

-

L

-

N

T

T

K

D

F

L

V

S

H

K

V

L

P

L

G

Q

S

D

L

I

D

D

Y

V

I

V

L

Y

H

H

F
x
L

A
|
A

S
x
A

-

I

P

P

A

G

S

V

P

R

I

T

D

S

Y

W

L

G

K

K

I

D

P

F

I

Q

Q

P

T

Y

L

V

K

T

V

N

G

N

A

I

L

M

G

V

T

T

H

Q

N

Q

L

L

G

E

L

A

A

C

K

K

D

H

-

I

K

K

G

L

A

D

R

K

F

F

M

I

I

H

A
x
I

S

S

T
|
T

S
|
S

E
x
S

I

V

Y

Y

G

G

D

-

P

-

L

-

V

-

H

-

P

-

Q

-

T

-

E

-

E

E

Y

K

Y

S

G

G

N

A

V

V

N

S

T

E

-

D

-

L

-

P

-

I

-

P

I

L

G

S

P

P

R
x
Y

G

G

V

V

Y

-

D

-

E

-

A

T

K
|
K

R

L

F

S

Q

G

E

E

S

H

I

L

T

C

M

H

A

V

Y

Y

H

H

T

K

F

N

H

F

G

H

L

I

D

P

T

I

R

V

I

I

V

L

R

R

I
x
Y

F

F

N
x
T

T
x
V

Y

Y

G

G

P

P

R

R

M

Q

R
|
R

L

P

N

D

D

-

G

-

R
x
M

V
x
A

I

F

P

H

A
x
R

F

L

M

I

G

K

Q

Q

A

M

L

L

R

E

G

D

E

K

D

P

L

L

T
|
T

V

I

F
|
F

G

G

D

D

G

G

S

T

Q
|
Q

T

T

R
|
R

S

D

F

F

C

T

F

Y

V

I

D

D

E

C

I

I

E

R

G

G

I

T

Y

V

R

A

L

A

L

L

M

E

S

T

D

K

Y

K

N

N

L

I

-

I

-

G

-

E

P

V

M

I

N

N

I

I

G

G

N

G

P

K

H

E

E

Q

I

A

T

S

I
|
I

K

L

D

D

F

I

A

I

E

S

E

M

I

L

I

E

K

K

L

I

T

S

G

G

-

K

T

S

D

A

Q

T

K

K

I

N

I

F

Y

L

K

K

P

S

L

V

P

P

K

-

D

G

D
x
E

P

P

M

K

Q

Q

R

T

Q

W

P

A

D

D

I

I

T

S

K

K

A

A

R

S

E

T

I

L

L

L

G

Q

W

Y

E

S

P

P

K

T

V

V

G

S

R

L

E

S

E

D

G

G

M

L

R

E

K

A

T

E

F

Y

E

D

F

Y

F

I

K

K

T

Q

L

L

active site: T126 (= T117), S127 (= S118), S128 (≠ E119), Y149 (≠ R143), K153 (= K150)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (≠ L13), D32 (= D32), H33 (≠ N33), F34 (≠ L34), I35 (= I35), K43 (≠ D38), D62 (= D57), I63 (≠ V58), L81 (≠ F74), A82 (= A75), A83 (≠ S76), I124 (≠ A115), T126 (= T117), K153 (= K150), Y176 (≠ I173), T178 (≠ N175), V179 (≠ T176), R185 (= R182), M188 (≠ R187)
binding uridine-5'-diphosphate: T178 (≠ N175), A189 (≠ V188), R192 (≠ A191), T204 (= T203), F206 (= F205), Q211 (= Q210), R213 (= R212), I250 (= I246), E276 (≠ D272)

6zljA Crystal structure of udp-glucuronic acid 4-epimerase y149f mutant from bacillus cereus in complex with udp-4-deoxy-4-fluoro-glucuronic acid and NAD (see paper)
31% identity, 94% coverage: 3:309/328 of query aligns to 2:313/314 of 6zljA

query
sites
6zljA

K

K

T

I

L

L

I

V

T

T

G
|
G

A

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

H

L

L

C

C

D

Q

R

A

F

L

L

L

A

K

E

N

G

S

H

A

Y

Y

-

H

V

V

I

V

G

G

M

I

D
|
D

N
x
H

L
x
F

I
|
I

-

G

-

P

-

T

-

P

-

A

T

T

G

L

D
x
K

L

T

K

G

N

N

I

I

E

Q

H

S

L

L

F

E

G

L

H

N

K

S

N

R

F

F

E

Q

F

F

A

I

H

R

H

E

D
|
D

V
x
I

-

L

-

N

T

T

K

D

F

L

V

S

H

K

V

L

P

L

G

Q

S

D

L

I

D

D

Y

V

I

V

L

Y

H

H

F
x
L

A
|
A

S
x
A

-

I

P
|
P

A

G

S

V

P
x
R

I

T

D

S

Y

W

L

G

K

K

I

D

P

F

I

Q

Q

P

T

Y

L

V

K

T

V

N

G

N

A

I

L

M

G

V

T

T

H

Q

N

Q

L

L

G

E

L

A

A

C

K

K

D

H

-

I

K

K

G

L

A

D

R

K

F

F

M

I

I

H

A
x
I

S

S

T
|
T

S
|
S

E
x
S

I

V

Y

Y

G

G

D

-

P

-

L

-

V

-

H

-

P

-

Q

-

T

-

E

-

E

E

Y

K

Y

S

G

G

N

A

V

V

N

S

T

E

-

D

-

L

-

P

-

I

-

P

I

L

G

S

P

P

R
x
F

G

G

V

V

Y

-

D

-

E

-

A

T

K
|
K

R

L

F

S

Q

G

E

E

S

H

I

L

T

C

M

H

A

V

Y

Y

H

H

T

K

F

N

H

F

G

H

L

I

D

P

T

I

R

V

I

I

V

L

R

R

I
x
Y

F
|
F

N
x
T

T
x
V

Y

Y

G

G

P

P

R

R

M

Q

R
|
R

L

P

N

D

D

-

G

-

R
x
M

V
x
A

I

F

P

H

A
x
R

F

L

M

I

G

K

Q

Q

A

M

L

L

R

E

G

D

E

K

D

P

L

L

T
|
T

V

I

F
|
F

G

G

D

D

G

G

S

T

Q
|
Q

T

T

R
|
R

S

D

F

F

C

T

F

Y

V

I

D

D

E

C

I

I

E

R

G

G

I

T

Y

V

R

A

L

A

L

L

M

E

S

T

D

K

Y

K

N

N

L

I

-

I

-

G

-

E

P

V

M

I

N

N

I

I

G

G

N

G

P

K

H

E

E

Q

I

A

T

S

I
|
I

K

L

D

D

F

I

A

I

E

S

E

M

I

L

I

E

K

K

L

I

T

S

G

G

-

K

T

S

D

A

Q

T

K

K

I

N

I

F

Y

L

K

K

P

S

L

V

P

P

K

-

D

G

D
x
E

P

P

M

K

Q

Q

R

T

Q

W

P

A

D

D

I

I

T

S

K

K

A

A

R

S

E

T

I

L

L

L

G

Q

W

Y

E

S

P

P

K

T

V

V

G

S

R

L

E

S

E

D

G

G

M

L

R

E

K

A

T

E

F

Y

E

D

F

Y

F

I

K

K

T

Q

L

L

active site: T126 (= T117), S127 (= S118), S128 (≠ E119), F149 (≠ R143), K153 (= K150)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (≠ L13), D32 (= D32), H33 (≠ N33), F34 (≠ L34), I35 (= I35), K43 (≠ D38), D62 (= D57), I63 (≠ V58), L81 (≠ F74), A82 (= A75), A83 (≠ S76), I124 (≠ A115), T126 (= T117), K153 (= K150), Y176 (≠ I173), T178 (≠ N175), V179 (≠ T176), R185 (= R182), M188 (≠ R187)
binding (2~{R},3~{S},4~{R},5~{R},6~{R})-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-fluoranyl-4,5-bis(oxidanyl)oxane-2-carboxylic acid: P85 (= P77), R88 (≠ P80), T126 (= T117), S127 (= S118), S128 (≠ E119), F149 (≠ R143), F177 (= F174), T178 (≠ N175), R185 (= R182), M188 (≠ R187), A189 (≠ V188), R192 (≠ A191), T204 (= T203), F206 (= F205), Q211 (= Q210), R213 (= R212), I250 (= I246), E276 (≠ D272)

3ruhA Alternative analogs as viable substrates of udp-hexose 4-epimerases
30% identity, 95% coverage: 2:311/328 of query aligns to 17:336/336 of 3ruhA

query
sites
3ruhA

K

K

K

T

T

W

L

L

I

I

T

T

G
|
G

A

V

A

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

N

L

L

C

L

D

E

R

K

F

L

L

L

A

K

E

L

G

N

H

Q

Y

V

V

V

I

I

G

G

M

L

D
|
D

N
|
N

L

F

I
x
S

T
|
T

G
|
G

-

H

-

Q

-

Y

D

N

L

L

K

D

N

E

I

V

E

K

H

T

L

L

F

V

G

S

H

T

K

E

N

Q

F

W

-

S

-

R

-

F

-

C

-

F

-

I

E

E

F

G

A
x
D

H
x
I

H

R

D

D

V

L

T

T

K

T

F

C

V

E

H

Q

V

V

P

M

G

K

S

G

L

V

D

D

Y

H

I

V

L

L

H

H

F
x
Q

A
|
A

S
x
A

P

L

A
x
G

S
|
S

P

V

I

P

D

R

Y

S

L

I

K

V

I

D

P

P

I

I

Q

T

T

T

L

N

K

A

V
x
T

G

N

A

I

L

T

G

G

T

F

H

L

N

N

L

I

L

L

G

H

L

A

A

A

K

K

D

N

K

A

G

Q

A

V

R

Q

-

S

F

F

M

T

I

Y

A
|
A

S
x
A

T
x
S

S
|
S

E
x
S

I

T

Y

Y

G

G

D

D

P

H

L

P

V

A

H

L

P

P

Q

K

T

V

E

E

Y

N

Y

I

G

G

N

N

V

-

N

-

T

-

I

-

G

-

P

P

R

L

G

S

V

P

Y
|
Y

D

A

E

V

A

T

K
|
K

R

Y

F

V

Q

N

E

E

S

I

I

Y

T

A

M

Q

A

V

Y

Y

H

A

T

R

F

T

H

Y

G

G

L

F

D

K

T

T

R

I

I

G

V

L

R

R

I
x
Y

F

F

N
|
N

T
x
V

Y

F

G

G

P

R

R

R

M

Q

R

D

L

P

N
|
N

D

G

-

A

-

Y

G

A

R
x
A

V
|
V

I

I

P

P

A
x
K

F
x
W

M

T

G

A

Q

A

A

M

L

L

R

K

G

G

E

D

D

D

L

V

T
x
Y

V
x
I

F
x
N

G

G

D

D

G

G

S

E

Q

T

T

S

R
|
R

S

D

F

F

C

C

F

Y

V

I

D

D

D

N

E

V

I

I

E

Q

-

M

G

N

I

I

Y

L

R

S

L

A

L

L

M

A

S

K

D

D

Y

S

-

A

-

K

N

D

L

N

P

I

M

Y

N

N

I

V

G

A

N

V

P

G

H

D

E

R

I

T

T

T

I
x
L

K

N

D

E

F

L

A

S

E

G

E

Y

I

I

I

Y

K

D

L

E

T

L

G

N

T

L

D

I

Q

H

K

H

I

I

I

K

Y

Y

K

R

P

E

L

F

P
x
R

K

S

D

G

D
|
D

P
x
V

M

R

Q

H

R
x
S

Q

Q

P

A

D

D

I

V

T

T

K

K

A

A

R

I

E

D

I

L

L

L

G

K

W

Y

E

R

P

P

K

N

V

I

G

K

R

I

E

R

E

E

G

G

M

L

R

R

K

L

T

S

F

M

E

P

F

W

F

Y

K

V

T

R

L

F

T

L

K

K

active site: S142 (≠ T117), S143 (= S118), S144 (≠ E119), Y166 (= Y146), K170 (= K150), N204 (= N184)
binding nicotinamide-adenine-dinucleotide: G23 (= G8), G26 (= G11), F27 (= F12), I28 (≠ L13), D47 (= D32), N48 (= N33), S50 (≠ I35), T51 (= T36), G52 (= G37), D78 (≠ A54), I79 (≠ H55), Q98 (≠ F74), A99 (= A75), A100 (≠ S76), T117 (≠ V93), A140 (= A115), A141 (≠ S116), S142 (≠ T117), Y166 (= Y146), K170 (= K150), Y193 (≠ I173), V196 (≠ T176)
binding [[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl][(2R,3R,4R,5R,6R)-6-(hydroxymethyl)-4,5-bis(oxidanyl)-3-(2-oxidanylidenepropyl)oxan-2-yl] hydrogen phosphate: G102 (≠ A78), S103 (= S79), S142 (≠ T117), S143 (= S118), S144 (≠ E119), Y166 (= Y146), Y193 (≠ I173), N195 (= N175), A209 (≠ R187), V210 (= V188), K213 (≠ A191), W214 (≠ F192), Y225 (≠ T203), I226 (≠ V204), N227 (≠ F205), R234 (= R212), L271 (≠ I246), R294 (≠ P269), D297 (= D272), V298 (≠ P273), S301 (≠ R276)

3rufA Alternative analogs as viable substrates of udp-hexose 4-epimerases
30% identity, 95% coverage: 2:311/328 of query aligns to 17:336/336 of 3rufA

query
sites
3rufA

K

K

K

T

T

W

L

L

I

I

T

T

G
|
G

A

V

A

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

N

L

L

C

L

D

E

R

K

F

L

L

L

A

K

E

L

G

N

H

Q

Y

V

V

V

I

I

G

G

M

L

D
|
D

N
|
N

L

F

I
x
S

T
|
T

G
|
G

-

H

-

Q

-

Y

D

N

L

L

K

D

N

E

I

V

E

K

H

T

L

L

F

V

G

S

H

T

K

E

N

Q

F

W

-

S

-

R

-

F

-

C

-

F

-

I

E

E

F

G

A
x
D

H
x
I

H

R

D

D

V

L

T

T

K

T

F

C

V

E

H

Q

V

V

P

M

G

K

S

G

L

V

D

D

Y

H

I

V

L

L

H

H

F
x
Q

A
|
A

S
x
A

P

L

A

G

S

S

P

V

I

P

D

R

Y

S

L

I

K

V

I

D

P

P

I

I

Q

T

T

T

L

N

K

A

V
x
T

G

N

A

I

L

T

G

G

T

F

H

L

N

N

L

I

L

L

G

H

L

A

A

A

K

K

D

N

K

A

G

Q

A

V

R

Q

-

S

F

F

M

T

I

Y

A
|
A

S

A

T
x
S

S
|
S

E
x
S

I

T

Y

Y

G

G

D

D

P

H

L

P

V

A

H

L

P

P

Q

K

T

V

E

E

Y

N

Y

I

G

G

N

N

V

-

N

-

T

-

I

-

G

-

P

P

R

L

G

S

V

P

Y
|
Y

D

A

E

V

A

T

K
|
K

R

Y

F

V

Q

N

E

E

S

I

I

Y

T

A

M

Q

A

V

Y

Y

H

A

T

R

F

T

H

Y

G

G

L

F

D

K

T

T

R

I

I

G

V

L

R

R

I
x
Y

F

F

N
|
N

T
x
V

Y

F

G

G

P

R

R

R

M

Q

R

D

L

P

N
|
N

D

G

-

A

-

Y

G

A

R
x
A

V
|
V

I

I

P

P

A
x
K

F
x
W

M

T

G

A

Q

A

A

M

L

L

R

K

G

G

E

D

D

D

L

V

T
x
Y

V
x
I

F
x
N

G

G

D

D

G

G

S

E

Q

T

T

S

R
|
R

S

D

F

F

C

C

F

Y

V

I

D

D

D

N

E

V

I

I

E

Q

-

M

G

N

I

I

Y

L

R

S

L

A

L

L

M

A

S

K

D

D

Y

S

-

A

-

K

N

D

L

N

P

I

M

Y

N

N

I

V

G

A

N

V

P

G

H

D

E

R

I

T

T

T

I
x
L

K

N

D

E

F

L

A

S

E

G

E

Y

I

I

I

Y

K

D

L

E

T

L

G

N

T

L

D

I

Q

H

K

H

I

I

I

K

Y

Y

K

R

P

E

L

F

P
x
R

K

S

D

G

D
|
D

P

V

M

R

Q

H

R

S

Q

Q

P

A

D

D

I

V

T

T

K

K

A

A

R

I

E

D

I

L

L

L

G

K

W

Y

E

R

P

P

K

N

V

I

G

K

R

I

E

R

E

E

G

G

M

L

R

R

K

L

T

S

F

M

E

P

F

W

F

Y

K

V

T

R

L

F

T

L

K

K

active site: S142 (≠ T117), S143 (= S118), S144 (≠ E119), Y166 (= Y146), K170 (= K150), N204 (= N184)
binding nicotinamide-adenine-dinucleotide: G23 (= G8), G26 (= G11), F27 (= F12), I28 (≠ L13), D47 (= D32), N48 (= N33), S50 (≠ I35), T51 (= T36), G52 (= G37), D78 (≠ A54), I79 (≠ H55), Q98 (≠ F74), A99 (= A75), A100 (≠ S76), T117 (≠ V93), A140 (= A115), Y166 (= Y146), K170 (= K150), Y193 (≠ I173), V196 (≠ T176)
binding uridine-5'-diphosphate: N195 (= N175), A209 (≠ R187), V210 (= V188), K213 (≠ A191), W214 (≠ F192), Y225 (≠ T203), I226 (≠ V204), N227 (≠ F205), R234 (= R212), L271 (≠ I246), R294 (≠ P269), D297 (= D272)

3lu1A Crystal structure analysis of wbgu: a udp-galnac 4-epimerase (see paper)
30% identity, 95% coverage: 2:311/328 of query aligns to 17:336/336 of 3lu1A

query
sites
3lu1A

K

K

K

T

T

W

L

L

I

I

T

T

G
|
G

A

V

A

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

N

L

L

C

L

D

E

R

K

F

L

L

L

A

K

E

L

G

N

H

Q

Y

V

V

V

I

I

G

G

M

L

D
|
D

N
|
N

L

F

I
x
S

T
|
T

G
|
G

-

H

-

Q

-

Y

D

N

L

L

K

D

N

E

I

V

E

K

H

T

L

L

F

V

G

S

H

T

K

E

N

Q

F

W

-

S

-

R

-

F

-

C

-

F

-

I

E

E

F

G

A
x
D

H
x
I

H

R

D

D

V

L

T

T

K

T

F

C

V

E

H

Q

V

V

P

M

G

K

S

G

L

V

D

D

Y

H

I

V

L

L

H

H

F
x
Q

A
|
A

S
x
A

P

L

A

G

S
|
S

P

V

I

P

D

R

Y

S

L

I

K

V

I

D

P

P

I

I

Q

T

T

T

L

N

K

A

V

T

G

N

A

I

L

T

G

G

T

F

H

L

N

N

L

I

L

L

G

H

L

A

A

A

K

K

D

N

K

A

G

Q

A

V

R
x
Q

-

S

F

F

M

T

I

Y

A
|
A

S
x
A

T
x
S

S
|
S

E
x
S

I

T

Y

Y

G

G

D

D

P

H

L

P

V

A

H

L

P

P

Q

K

T

V

E

E

Y

N

Y

I

G

G

N

N

V

-

N

-

T

-

I

-

G

-

P

P

R

L

G

S

V

P

Y
|
Y

D

A

E

V

A

T

K
|
K

R

Y

F

V

Q

N

E

E

S

I

I

Y

T

A

M

Q

A

V

Y

Y

H

A

T

R

F

T

H

Y

G

G

L

F

D
x
K

T

T

R

I

I

G

V

L

R

R

I
x
Y

F

F

N
|
N

T

V

Y

F

G

G

P

R

R

R

M

Q

R

D

L

P

N
|
N

D

G

-

A

-

Y

G

A

R

A

V
|
V

I

I

P

P

A

K

F
x
W

M

T

G

A

Q

A

A

M

L

L

R

K

G

G

E

D

D

D

L

V

T
x
Y

V
x
I

F
x
N

G

G

D

D

G

G

S

E

Q

T

T

S

R
|
R

S

D

F

F

C

C

F

Y

V

I

D

D

D

N

E

V

I

I

E

Q

-

M

G

N

I

I

Y

L

R

S

L

A

L

L

M

A

S

K

D

D

Y

S

-

A

-

K

N

D

L

N

P

I

M

Y

N

N

I

V

G

A

N

V

P

G

H

D

E

R

I

T

T

T

I
x
L

K

N

D

E

F

L

A

S

E

G

E

Y

I

I

I

Y

K

D

L

E

T

L

G

N

T

L

D

I

Q

H

K

H

I

I

I

K

Y

Y

K

R

P

E

L

F

P
x
R

K

S

D

G

D
|
D

P

V

M

R

Q

H

R

S

Q

Q

P

A

D

D

I

V

T

T

K

K

A

A

R

I

E

D

I

L

L

L

G

K

W

Y

E

R

P

P

K

N

V

I

G

K

R

I

E

R

E

E

G

G

M

L

R

R

K

L

T

S

F

M

E

P

F

W

F

Y

K

V

T

R

L

F

T

L

K

K

active site: S142 (≠ T117), S143 (= S118), S144 (≠ E119), Y166 (= Y146), K170 (= K150), N204 (= N184)
binding glycine: Q135 (≠ R111), K187 (≠ D167)
binding nicotinamide-adenine-dinucleotide: G23 (= G8), G26 (= G11), F27 (= F12), I28 (≠ L13), D47 (= D32), N48 (= N33), S50 (≠ I35), T51 (= T36), G52 (= G37), D78 (≠ A54), I79 (≠ H55), Q98 (≠ F74), A99 (= A75), A100 (≠ S76), A140 (= A115), A141 (≠ S116), S142 (≠ T117), Y166 (= Y146), K170 (= K150), Y193 (≠ I173), N195 (= N175)
binding uridine-diphosphate-n-acetylgalactosamine: S103 (= S79), S142 (≠ T117), S143 (= S118), S144 (≠ E119), Y166 (= Y146), N195 (= N175), V210 (= V188), W214 (≠ F192), Y225 (≠ T203), I226 (≠ V204), N227 (≠ F205), R234 (= R212), L271 (≠ I246), R294 (≠ P269), D297 (= D272)

Q9SYM5 Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase/UDP-4-keto-L-rhamnose-reductase RHM1; Protein REPRESSOR OF LRX1 1; Rhamnose biosynthetic enzyme 1; AtRHM1; EC 4.2.1.76; EC 1.1.1.-; EC 5.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
30% identity, 93% coverage: 2:306/328 of query aligns to 7:319/669 of Q9SYM5

query
sites
Q9SYM5

K

K

K

N

T

I

L

L

I

I

T

T

G

G

A

A

G

G

F

F

L

I

G

A

S

S

H

H

L

V

C

A

D

N

R

R

F

L

L

I

A

R

E

S

-

Y

-

P

G

D

H

Y

Y

K

V

I

I

V

G

V

M

L

D

D

N

K

L

L

-

D

I

Y

T

C

G

S

D

N

L

L

K

K

N

N

I

L

E

N

H

P

L

S

F

K

G

H

H

S

K

P

N

N

F

F

E

K

F

F

-

V

-

K

-

G

-

D

-

I

A

A

H

S

H

A

D

D

V

L

T

V

K

N

F

H

V

L

H

L

V

I

P

T

G

E

S

G

L

I

D

D

Y

T

I

I

L

M

H

H

F

F

A

A

S

A

P

Q

A

T

S

H

P

V

I

D

D

N

Y

S

L

F

K

G

I

N

P

S

I

F

Q

E

T

F

L

T

K

K

V

N

G

N

A

I

L

Y

G

G

T

T

H

H

N

V

L

L

G

E

L

A

A

C

K

K

D

V

K

T

G

G

A

Q

-

I

-

R

R

R

F

F

M

I

I

H

A

V

S

S

T

T

S

D

E

E

I

V

Y

Y

G

G

D

E

P

T

L

-

V

-

H

-

P

-

Q

D

T

E

E

D

E

A

Y

L

Y

V

G

G

N

N

-

H

-

E

V

A

N

S

T

Q

I

L

G

L

P

P

R

T

G

N

V

P

Y

Y

D

S

E

A

A

T

K

K

R

A

F

G

Q

A

E

E

S

M

I

L

T

V

M

M

A

A

Y

Y

H

G

T

R

F

S

H

Y

G

G

L

L

D

P

T

V

R

I

I

T

V

T

R

R

I

G

F

N

N

N

T

V

Y

Y

G

G

P

P

R

N

M

Q

R

F

L

P

N

E

D

-

G

-

R

K

V

L

I

I

P

P

A

K

F

F

M

I

G

L

Q

L

A

A

L

M

R

R

G

G

E

Q

D

V

L

L

T

P

V

I

F

H

G

G

D

D

G

G

S

S

Q

N

T

V

R

R

S

S

F

Y

C

L

F

Y

V

C

D

E

D

D

E

V

I

A

E

E

G

A

I

F

Y

E

R

V

L

V

L

L

-

H

M

K

S

G

D

E

Y

V

N

G

L

H

P

V

M

Y

N

N

I

I

G

G

N

T

P

K

H

K

E

E

I

R

T

R

I

V

K

N

D

D

F

V

A

A

E

K

E

D

I

I

I

C

K

K

L

L

T

F

G

N

T

M

D

D

Q

P

K

E

I

A

I

N

Y

I

K

K

P

-

L

F

P

V

K

D

D

N

D
x
R

P

P

M

F

Q

N

R

D

Q

Q

P

R

D

Y

I

F

T

L

K

D

A

D

R

Q

E

K

I

L

-

K

L

L

G

G

W

W

E

S

P

E

K

R

V

T

G

T

R

W

E

E

G

G

M

L

R

K

K

K

T

T

F

M

E

D

F

W

F

Y

R283 (≠ D272) mutation to K: In rol1-2; Abolishes dehydratase activity in vitro (PubMed:16766693). Induces aberrant accumulation of flavonols leading to alterations in plant growth and cell shape formation (PubMed:18567791, PubMed:18757557).

4m55E Crystal structure of human udp-xylose synthase r236h substitution (see paper)
37% identity, 57% coverage: 3:188/328 of query aligns to 1:143/164 of 4m55E

query
sites
4m55E

K

R

T

I

L

L

I

I

T

T

G
|
G

A

G

A

A

G
|
G

F
|
F

L
x
V

G

G

S

S

H

H

L

L

C

T

D

D

R

K

F

L

L

M

A

M

E

D

G

G

H

H

Y

E

V

V

I

T

G

V

M

V

D
|
D

N
|
N

L
x
F

I

F

T
|
T

G
|
G

D

R

L

K

K

R

N

N

I

V

E

E

H

H

L

W

F

I

G

G

H

H

K

E

N

N

F

F

E

E

F

L

A

I

H

N

H

H

D
|
D

V
|
V

T

V

K

E

F

P

V

L

H

Y

V

I

P

-

G

-

S

E

L

V

D

D

Y

Q

I

I

L

Y

H

H

F

L

A

A

S
|
S

P
|
P

A

N

S

-

P

-

I

-

D

-

Y

-

L

-

K

-

I

-

P

P

I

I

Q

K

T

T

L

L

K

K

V
x
T

G

N

A

T

L

I

G

G

T

T

H

L

N

N

L

M

L

L

G

G

L

L

A

A

K

K

D

R

K

V

G

G

A

A

R

R

F

L

M

L

I

L

A

A

S

S

T
|
T

S

S

E

-

I

-

Y

-

G

-

D

-

P

-

L

-

V

-

H

-

P

-

Q

-

T

-

E

-

Y

-

G

-

N

-

V

-

N

-

T

-

I

-

G

-

P

-

R

-

G

-

V

-

Y

-

D

-

E

-

A

-

K

-

R

-

F

V

Q

A

E

E

S

T

I

M

T

C

M

Y

A

A

Y

Y

H

M

T

K

F

Q

H

E

G

G

L

V

D

E

T

V

R

R

I

V

V

A

R

R

I

I

F

F

N

N

T
|
T

Y

F

G

Q

P

Y

R

V

M

S

R

D

L

L

N

V

D

N

G

G

R

L

V

V

active site: T105 (= T117)
binding nicotinamide-adenine-dinucleotide: G6 (= G8), G9 (= G11), F10 (= F12), V11 (≠ L13), D30 (= D32), N31 (= N33), F32 (≠ L34), T34 (= T36), G35 (= G37), D55 (= D57), V56 (= V58), S72 (= S76), P73 (= P77), T81 (≠ V93), T105 (= T117), T131 (= T176)

7ystA Crystal structure of udp-glucose 4-epimerase (rv3634c) in complex with both udp-glucose and udp-galactose in chain b from mycobacterium tuberculosis
31% identity, 93% coverage: 3:307/328 of query aligns to 2:309/312 of 7ystA

query
sites
7ystA

K

R

T

A

L

L

I

V

T

T

G
|
G

A

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

T

L

L

C

V

D

D

R

R

F

L

L

L

A

A

E

D

G

G

H

H

Y

S

V

V

I

V

G

G

M

L

D
|
D

N
|
N

L

F

I

A

T
|
T

G
|
G

D

R

L

A

K

T

N

N

I

L

E

E

H

H

L

L

F

A

G

D

H

N

K

S

N

A

F

H

E

V

F

F

A

V

H

E

H

A

D
|
D

-
x
I

V

V

T

T

K

A

F

D

V

L

H

H

V

A

-

I

-

L

-

E

P

Q

G

H

S

R

L

P

D

E

Y

V

I

V

L

F

H

H

F
x
L

A
|
A

S
x
A

P

Q

A
x
I

S

D

P

V

I
x
R

D

R

Y

S

L

V

K

A

I

D

P

P

I

Q

Q

F

T

D

L

A

K

A

V
|
V

G

N

A

V

L

I

G

G

T

T

H

V

N

R

L

L

L

A

G

E

L

A

A

A

K

R

D

Q

K

T

G

G

A

V

R

R

F

K

M

I

I

V

A

H

S
x
T

T

S

S
|
S

-

G

-
x
G

E

S

I

I

Y

Y

G

G

D

T

P

P

L

P

V

E

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Y

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T

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E

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G

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x
A

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x
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x
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A

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x
F

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A

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Q

A

A

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L

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T
x
R

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x
R

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I

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M

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binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (≠ L13), D31 (= D32), N32 (= N33), T35 (= T36), G36 (= G37), D56 (= D57), I57 (vs. gap), L77 (≠ F74), A78 (= A75), A79 (≠ S76), I81 (≠ A78), V96 (= V93), T119 (≠ S116), Y146 (= Y146), K150 (= K150), P173 (≠ I173), A174 (≠ F174), N175 (= N175), V176 (≠ T176)
binding uridine-5'-diphosphate-glucose: I81 (≠ A78), R84 (≠ I81), S121 (= S118), G123 (vs. gap), Y146 (= Y146), A174 (≠ F174), N175 (= N175), A187 (≠ R187), G188 (≠ V188), V189 (≠ I189), F193 (≠ M193), R204 (≠ T203), V205 (= V204), F206 (= F205), R213 (= R212), D248 (≠ I246), R271 (≠ P269)

7ysmA Crystal structure of udp-glucose 4-epimerase (rv3634c) co-crystallized with udp-n-acetylglucosamine from mycobacterium tuberculosis
31% identity, 93% coverage: 3:307/328 of query aligns to 2:309/311 of 7ysmA

query
sites
7ysmA

K

R

T

A

L

L

I

V

T

T

G
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G

A

A

G
|
G

F
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F

L
x
I

G

G

S

S

H

T

L

L

C

V

D

D

R

R

F

L

L

L

A

A

E

D

G

G

H

H

Y

S

V

V

I

V

G

G

M

L

D
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D

N
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N

L

F

I

A

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T

G
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G

D

R

L

A

K

T

N

N

I

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E

H

H

L

L

F

A

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H

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K

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N

A

F

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F

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H

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D

-
x
I

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I

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A

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A

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A

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x
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W

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F

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binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (≠ L13), D31 (= D32), N32 (= N33), T35 (= T36), G36 (= G37), D56 (= D57), I57 (vs. gap), L77 (≠ F74), A78 (= A75), A79 (≠ S76), I81 (≠ A78), T119 (≠ S116), Y146 (= Y146), K150 (= K150), P173 (≠ I173), N175 (= N175), V176 (≠ T176)
binding uridine-diphosphate-n-acetylgalactosamine: I81 (≠ A78), R84 (≠ I81), S121 (= S118), G123 (vs. gap), S124 (≠ E119), Y146 (= Y146), A174 (≠ F174), N175 (= N175), G188 (≠ V188), V189 (≠ I189), F193 (≠ M193), R204 (≠ T203), V205 (= V204), F206 (= F205), N211 (≠ Q210), R213 (= R212), D248 (≠ I246), R271 (≠ P269)

Query Sequence

>WP_054559941.1 NCBI__GCF_001306415.1:WP_054559941.1
MKKTLITGAAGFLGSHLCDRFLAEGHYVIGMDNLITGDLKNIEHLFGHKNFEFAHHDVTK
FVHVPGSLDYILHFASPASPIDYLKIPIQTLKVGALGTHNLLGLAKDKGARFMIASTSEI
YGDPLVHPQTEEYYGNVNTIGPRGVYDEAKRFQESITMAYHTFHGLDTRIVRIFNTYGPR
MRLNDGRVIPAFMGQALRGEDLTVFGDGSQTRSFCFVDDEIEGIYRLLMSDYNLPMNIGN
PHEITIKDFAEEIIKLTGTDQKIIYKPLPKDDPMQRQPDITKAREILGWEPKVGREEGMR
KTFEFFKTLTKEELERTEHRDFSKINRK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory