SitesBLAST

Q8RDH4 Dipeptide transport ATP-binding protein DppD; EC 7.4.2.9 from Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis) (see paper)
31% identity, 96% coverage: 11:347/350 of query aligns to 4:316/326 of Q8RDH4

query
sites
Q8RDH4

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ASGKST 44:49 (≠ GCGKTT 55:60) binding ATP
N61 (≠ T72) binding ATP
Q97 (= Q126) binding ATP
C285 (= C316) binding [4Fe-4S] cluster
C291 (= C322) binding [4Fe-4S] cluster
C298 (= C329) binding [4Fe-4S] cluster
C316 (= C347) binding [4Fe-4S] cluster

P0AAH4 Putrescine export system ATP-binding protein SapD from Escherichia coli (strain K12) (see paper)
30% identity, 96% coverage: 11:347/350 of query aligns to 3:321/330 of P0AAH4

query
sites
P0AAH4

L

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K46 (= K58) mutation K->G,I,Q,R: 25% to 50% stimulation of K(+) uptake by TrkH.; mutation Missing: Almost no stimulation of K(+) uptake by TrkH.
G162 (= G186) mutation to A: 80% stimulation of K(+) uptake by TrkH.
Q165 (= Q189) mutation to L: 60% stimulation of K(+) uptake by TrkH.
D183 (= D207) mutation to E: About 60% stimulation of K(+) uptake by TrkH.; mutation D->K,N: 15% to 20% stimulation of K(+) uptake by TrkH.

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 76% coverage: 11:277/350 of query aligns to 1:235/343 of P30750

query
sites
P30750

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P

40:46 (vs. 54:60, 71% identical) binding ATP
E166 (= E208) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
31% identity, 77% coverage: 10:277/350 of query aligns to 1:236/344 of 6cvlD

query
sites
6cvlD

Y

H

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P

binding phosphothiophosphoric acid-adenylate ester: F12 (= F21), Q14 (= Q25), I19 (= I30), S41 (= S54), G42 (= G55), A43 (≠ C56), G44 (= G57), K45 (= K58), S46 (≠ T59), T47 (= T60), N141 (≠ E182), S143 (= S184), Q146 (= Q187), H200 (= H241)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
31% identity, 77% coverage: 10:277/350 of query aligns to 1:236/344 of 3tuzC

query
sites
3tuzC

Y

H

L

M

L

I

E

K

V

L

E

S

H

N

L

I

K

T

Q

K

Y

V

F
|
F

P

-

V

-

H

H

Q
|
Q

G

G

F

T

S

R

T

T

I

I

P

-

L

-

K

Q

A

A

V

L

D

N

I

V

S

S

F

L

A

H

I

V

R

P

P

A

G

G

E

Q

T

I

L

Y

G

G

L

V

V

I

G

G

E

A

S

S

G
|
G

C

A

G
|
G

K
|
K

T
x
S

T
|
T

V

L

G

I

R

R

T

C

L

V

L

N

R

L

L

L

Y

E

Q

R

P

P

T

T

A

E

G

G

K

S

I

V

T

L

F

V

D

D

G

G

K

Q

V

E

L

L

F

T

D

T

S

L

G

S

E

E

Q

S

Y

-

D

-

E

-

N

-

G

-

K

-

M

-

I

-

V

-

D

-

A

-

N

-

G

-

K

-

P

-

V

-

M

-

G

-

K

-

V

-

D

-

V

-

N

E

M

L

M

T

P

K

Y

A

R

R

K

R

E

Q

M

I

Q

G

M

M

V

I

F

F

Q

Q

D

H

P

-

Y

F

S

N

S

L

L

L

N

S

P

S

R

R

M

-

T

T

V

V

E

F

D

G

I

N

I

V

G

A

E

L

P

P

L

L

D

E

V

L

H

D

K

N

L

T

Y

P

R

K

N

D

R

E

K

V

E

K

R

R

E

-

K

-

I

V

L

T

D

E

L

L

M

L

E

S

L

L

V

V

G

G

L

L

N

G

A

D

E

K

H

H

A

D

M

-

R

S

Y

Y

A

P

H

S

E

N

F

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

I

V

G

A

I

I

A

A

R

R

A

A

L

L

A

A

V

S

N

N

P

P

K

K

F

V

I

L

V

L

C

C

D

D

E

Q

A

A

V

T

S

S

A

A

L

L

D

D

V

P

S

A

I

T

Q

T

A

R

Q

S

V

I

V

L

N

E

M

L

F

L

E

K

E

D

L

I

Q

N

E

R

K

R

L

L

G

G

V

L

A

T

Y

I

L

L

F

L

I

I

A

T

H

H

D

E

L

M

L

D

V

V

H

K

H

R

I

I

S

C

D

D

R

C

I

V

A

A

V

V

M

I

Y

S

L

N

G

G

K

E

M

L

M

I

E

E

I

Q

A

D

D

T

A

V

D

S

E

E

L

V

N

F

A

S

N

H

P

P

I

K

H

T

P

P

binding adenosine-5'-diphosphate: F12 (= F21), Q14 (= Q25), G42 (= G55), G44 (= G57), K45 (= K58), S46 (≠ T59), T47 (= T60)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
31% identity, 77% coverage: 10:277/350 of query aligns to 1:236/344 of 3tuiC

query
sites
3tuiC

Y

H

L

M

L

I

E

K

V

L

E

S

H

N

L

I

K

T

Q

K

Y

V

F
|
F

P

-

V

-

H

H

Q

Q

G

G

F

T

S

R

T

T

I

I

P

-

L

-

K

Q

A

A

V

L

D

N

I

V

S

S

F

L

A

H

I

V

R

P

P

A

G

G

E

Q

T

I

L

Y

G

G

L

V

V

I

G

G

E

A

S

S

G
|
G

C
x
A

G
|
G

K
|
K

T
x
S

T
|
T

V

L

G

I

R

R

T

C

L

V

L

N

R

L

L

L

Y

E

Q

R

P

P

T

T

A

E

G

G

K

S

I

V

T

L

F

V

D

D

G

G

K

Q

V

E

L

L

F

T

D

T

S

L

G

S

E

E

Q

S

Y

-

D

-

E

-

N

-

G

-

K

-

M

-

I

-

V

-

D

-

A

-

N

-

G

-

K

-

P

-

V

-

M

-

G

-

K

-

V

-

D

-

V

-

N

E

M

L

M

T

P

K

Y

A

R

R

K

R

E

Q

M

I

Q

G

M

M

V

I

F

F

Q

Q

D

H

P

-

Y

F

S

N

S

L

L

L

N

S

P

S

R

R

M

-

T

T

V

V

E

F

D

G

I

N

I

V

G

A

E

L

P

P

L

L

D

E

V

L

H

D

K

N

L

T

Y

P

R

K

N

D

R

E

K

V

E

K

R

R

E

-

K

-

I

V

L

T

D

E

L

L

M

L

E

S

L

L

V

V

G

G

L

L

N

G

A

D

E

K

H

H

A

D

M

-

R

S

Y

Y

A

P

H

S

E

N

F

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

I

V

G

A

I

I

A

A

R

R

A

A

L

L

A

A

V

S

N

N

P

P

K

K

F

V

I

L

V

L

C

C

D

D

E

Q

A

A

V

T

S

S

A

A

L

L

D

D

V

P

S

A

I

T

Q

T

A

R

Q

S

V

I

V

L

N

E

M

L

F

L

E

K

E

D

L

I

Q

N

E

R

K

R

L

L

G

G

V

L

A

T

Y

I

L

L

F

L

I

I

A

T

H

H

D

E

L

M

L

D

V

V

H

K

H

R

I

I

S

C

D

D

R

C

I

V

A

A

V

V

M

I

Y

S

L

N

G

G

K

E

M

L

M

I

E

E

I

Q

A

D

D

T

A

V

D

S

E

E

L

V

N

F

A

S

N

H

P

P

I

K

H

T

P

P

binding adenosine-5'-diphosphate: F12 (= F21), G42 (= G55), A43 (≠ C56), G44 (= G57), K45 (= K58), S46 (≠ T59), T47 (= T60)

2d62A Crystal structure of multiple sugar binding transport atp-binding protein
33% identity, 73% coverage: 34:288/350 of query aligns to 21:248/375 of 2d62A

query
sites
2d62A

A

A

V

V

D

K

D

D

I

L

S

S

F

L

A

E

I

I

R

K

P

D

G

G

E

E

T

F

L

L

G

V

L

L

V

L

G

G

E

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

V

T

G

L

R

R

T

M

L

I

L

A

R

G

L

L

Y

E

Q

E

P

P

T

T

A

R

G

G

K

Q

I

I

T

Y

F

I

D

E

G

D

K

N

V

L

L

V

F

A

D

D

S

P

G

-

E

-

Q

-

Y

-

D

-

E

E

N

K

G

G

K

-

M

-

I

-

V

-

D

-

A

-

N

-

G

-

K

-

P

-

V

-

M

-

G

-

K

-

V

-

D

-

V

V

N

F

M

V

M

P

P

P

Y

K

R

E

K

R

E

D

M

V

Q

A

M

M

V

V

F

F

Q

Q

D

S

P

-

Y

-

S

Y

S

A

L

L

N

Y

P

P

R

H

M

M

T

T

V

V

E

Y

D

D

I

N

I

I

G

A

E

F

P

P

L

L

D

K

V

L

H

R

K

K

L

V

Y

P

R

K

N

Q

R

E

K

I

E

D

R

K

R

R

E

-

K

-

I

V

L

R

D

E

L

V

M

A

E

E

L

M

V

L

G

G

L

L

N

T

A

-

E

E

H

L

A

L

M

N

R

R

Y

K

A

P

H

R

E

E

F

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

I

V

G

A

I

L

A

G

R

R

A

A

L

I

A

I

V

R

N

R

P

P

K

K

F

V

I

F

V

L

C

M

D

D

E

E

A

P

V

L

S

S

A

N

L

L

D

D

V

A

S

K

I

L

Q

R

A

V

Q

K

V

M

V

R

N

A

M

E

F

L

E

K

L

L

Q

Q

E

R

K

Q

L

L

G

G

V

V

A

T

Y

T

L

I

F

Y

I

V

A

T

H

H

D

D

L

Q

L

V

V

E

V

A

H

M

H

T

I

M

S

G

D

D

R

R

I

I

A

A

V

V

M

M

Y

N

L

K

G

G

K

E

M

L

M

Q

E

Q

I

V

A

G

D

T

A

P

D

D

E

E

L

V

N

Y

A

Y

N

K

P

P

I

V

H

N

P

T

Y

F

T

V

L

A

S

G

L

F

L

I

S

G

A

S

V

P

P

P

I

M

binding pyrophosphate 2-: G42 (= G55), C43 (= C56), G44 (= G57), K45 (= K58), T46 (= T59), T47 (= T60)

7z18I E. Coli c-p lyase bound to a phnk abc dimer and atp (see paper)
32% identity, 79% coverage: 11:286/350 of query aligns to 3:247/250 of 7z18I

query
sites
7z18I

L

L

E

S

V

V

E

N

H

N

L

L

K

T

Q

H

Y

L

F
x
Y

P

A

V

P

H

G

Q

K

G

G

F

F

S

S

T

-

I

-

P

-

L

-

K

-

A

-

V

-

D

-

D

D

I

V

S

S

F

F

A

D

I

L

R

W

P

P

G

G

E

E

T

V

L

L

G

G

L

I

V

V

G

G

E

E

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

T
|
T

V

L

G

L

R

K

T

S

L

I

L

S

R

A

L

R

Y

L

Q

T

P

P

T

Q

A

Q

G

G

K

E

I

I

T

H

F

Y

D

E

G

N

K

R

V

S

L

L

F

Y

D

A

S

M

G

S

E

E

Q

-

Y

-

D

-

E

-

N

-

G

-

K

-

M

-

I

-

V

-

D

-

A

A

N

D

G

R

K

R

P

R

V

L

M

L

G

-

K

-

V

-

D

-

V

-

N

-

M

-

P

-

Y

-

R

R

K

T

E

E

M

W

Q

G

M

V

V

V

F

H

Q
|
Q

D

H

P

P

Y

L

S

D

S

G

L

L

N

R

P

R

R

Q

M

V

T

S

V

A

E

G

D

G

I

N

I

I

G

G

E

E

P

R

L

L

D

-

V

M

H

A

K

T

L

G

Y

A

R

R

N

H

R

Y

K

G

E

D

R

I

R

R

E

A

K

T

I

A

L

Q

D

K

L

W

M

L

E

E

L

E

V

V

G

E

L

I

N

P

A

A

E

N

H
x
R

A

I

M

D

R

D

Y

L

A

P

H

T

E
x
T

F

F

S
|
S

G

G

G
|
G

Q
x
M

R

Q

Q

Q

R

R

I

L

G

Q

I

I

A

A

R

R

A

N

L

L

A

V

V

T

N

H

P

P

K

K

F

L

I

V

V

F

C

M

D

D

E

E

A

P

V

T

S

G

A

G

L

L

D

D

V

V

S

S

I

V

Q

Q

A

A

Q

R

V

L

N

D

M

L

F

L

E

R

E

G

L

L

Q

V

E

V

K

E

L

L

G

N

V

L

A

A

Y

V

L

V

F

I

I

V

A

T

H
|
H

D

D

L

L

L

G

V

V

V

A

H

R

H

L

I

L

S

A

D

D

R

R

I

L

A

L

V

V

M

M

Y

K

L

Q

G

G

K

Q

M

V

E

E

I

S

A

G

D

L

A

T

D

D

E

R

L

V

N

L

A

D

N

D

P

P

I

H

H

H

P

P

Y

Y

T

T

L

Q

S

L

L

L

L

V

S

S

A

S

V

V

binding adenosine-5'-triphosphate: Y13 (≠ F21), S38 (= S54), G39 (= G55), S40 (≠ C56), G41 (= G57), K42 (= K58), T43 (= T59), T44 (= T60), Q88 (= Q126), R136 (≠ H175), T143 (≠ E182), S145 (= S184), G147 (= G186), M148 (≠ Q187), H202 (= H241)
binding magnesium ion: T43 (= T59), Q88 (= Q126)

7z15I E. Coli c-p lyase bound to a phnk/phnl dual abc dimer and adp + pi (see paper)
32% identity, 79% coverage: 11:286/350 of query aligns to 3:247/253 of 7z15I

query
sites
7z15I

L

L

E

S

V

V

E

N

H

N

L

L

K

T

Q

H

Y

L

F
x
Y

P

A

V

P

H

G

Q
x
K

G
|
G

F

F

S

S

T

-

I

-

P

-

L

-

K

-

A

-

V

-

D

-

D

D

I

V

S

S

F

F

A

D

I

L

R

W

P

P

G

G

E

E

T

V

L

L

G

G

L

I

V

V

G

G

E

E

S
|
S

G
|
G

C

S

G
|
G

K
|
K

T
|
T

V

L

G

L

R

K

T

S

L

I

L

S

R

A

L

R

Y

L

Q

T

P

P

T

Q

A

Q

G

G

K

E

I

I

T

H

F

Y

D

E

G

N

K

R

V

S

L

L

F

Y

D

A

S

M

G

S

E

E

Q

-

Y

-

D

-

E

-

N

-

G

-

K

-

M

-

I

-

V

-

D

-

A

A

N

D

G

R

K

R

P

R

V

L

M

L

G

-

K

-

V

-

D

-

V

-

N

-

M

-

P

-

Y

-

R

R

K

T

E

E

M

W

Q

G

M

V

V

V

F

H

Q
|
Q

D

H

P

P

Y

L

S

D

S

G

L

L

N

R

P

R

R

Q

M

V

T

S

V

A

E

G

D

G

I

N

I

I

G

G

E

E

P

R

L

L

D

-

V

M

H

A

K

T

L

G

Y

A

R

R

N

H

R

Y

K

G

E

D

R

I

R

R

E

A

K

T

I

A

L

Q

D

K

L

W

M

L

E

E

L

E

V

V

G

E

L

I

N

P

A

A

E

N

H
x
R

A

I

M

D

R

D

Y

L

A

P

H

T

E
x
T

F

F

S
|
S

G

G

Q
x
M

R

Q

Q

Q

R

R

I

L

G

Q

I

I

A

A

R

R

A

N

L

L

A

V

V

T

N

H

P

P

K

K

F

L

I

V

V

F

C

M

D

D

E

E

A

P

V

T

S

G

A

G

L

L

D

D

V

V

S

S

I

V

Q

Q

A

A

Q

R

V

L

N

D

M

L

F

L

E

R

E

G

L

L

Q

V

E

V

K

E

L

L

G

N

V

L

A

A

Y

V

L

V

F

I

I

V

A

T

H

H

D

D

L

L

L

G

V

V

V

A

H

R

H

L

I

L

S

A

D

D

R

R

I

L

A

L

V

V

M

M

Y

K

L

Q

G

G

K

Q

M

V

E

E

I

S

A

G

D

L

A

T

D

D

E

R

L

V

N

L

A

D

N

D

P

P

I

H

H

H

P

P

Y

Y

T

T

L

Q

S

L

L

L

L

V

S

S

A

S

V

V

binding adenosine-5'-diphosphate: Y13 (≠ F21), K17 (≠ Q25), G18 (= G26), S38 (= S54), G39 (= G55), G41 (= G57), K42 (= K58), T43 (= T59), T44 (= T60), R136 (≠ H175), T143 (≠ E182), S145 (= S184), M148 (≠ Q187)
binding magnesium ion: T43 (= T59), Q88 (= Q126)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
35% identity, 70% coverage: 34:278/350 of query aligns to 41:258/382 of 7ahhC

query
sites
7ahhC

A
x
G

V

V

D

Y

D

D

I

T

S

N

F

F

A

E

I

I

R

N

P

E

G

G

E

E

T

I

L

F

G

V

L

I

V

M

G

G

E

L

S

S

G
|
G

C

S

G
|
G

K
|
K

T

S

T

T

V

L

G

L

R

R

T

L

L

L

L

N

R

R

L

L

Y

I

Q

E

P

P

T

T

A

S

G

G

K

K

I

I

T

F

F

I

D

D

G

-

K

-

V

-

L

-

F

-

D

-

S

-

G

-

E

-

Q

-

Y

-

D

-

E

-

N

-

G

-

K

-

M

-

I

-

V

-

D

-

A

-

N

N

G

Q

K

D

P

V

V

A

M

T

G

L

K

N

K

K

V

E

D

D

V

L

N

-

M

L

M

Q

P

V

Y

R

R

R

K

K

E

T

M

M

Q

S

M

M

V

V

F

F

Q

Q

D

N

P

-

Y

-

S

F

S

G

L

L

N

F

P

P

R

H

M

R

T

T

V

I

E

L

D

E

I

N

I

T

G

E

E

Y

P

G

L

L

D

E

V

V

H

Q

K

N

L

V

Y

P

R

K

N

E

-

E

R

R

K

R

E

K

R

R

R

A

E

E

K

K

I

A

L

L

D

D

L

N

M

A

E

N

L

L

V

L

G

D

L

F

N

K

A

D

E

Q

H

-

A

-

M

-

R

-

Y

Y

A

P

H

K

E

Q

F

L

S

S

G

G

Q

M

R

Q

Q

Q

R

R

I

V

G

G

I

L

A

A

R

R

A

A

L

L

A

A

V

N

N

D

P

P

K

E

F

I

I

L

V

L

C

M

D
|
D

E
|
E

A

A

V

F

S

S

A

A

L

L

D

D

V

P

S

L

I

I

Q

R

A

R

Q

E

V

M

V

Q

N

D

M

E

F

L

E

L

E

E

L

L

Q

Q

E

A

K

K

L

F

G

Q

V

K

A

T

Y

I

L

I

F

F

I

V

A

S

H

H

D

D

L

L

L

N

V

E

V

A

H

L

H

R

I

I

S

G

D

D

R

R

I

I

A

A

V

I

M

M

Y

K

L

D

G

G

K

K

M

I

M

M

E

Q

I

I

A

G

D

T

A

G

D

E

E

E

L

I

N

L

A

T

N

N

P

P

I

A

H

N

P

D

Y

Y

binding phosphoaminophosphonic acid-adenylate ester: G41 (≠ A34), G62 (= G55), G64 (= G57), K65 (= K58), D187 (= D207), E188 (= E208)

Sites not aligning to the query:

Query Sequence

>WP_055067293.1 NCBI__GCF_001406815.1:WP_055067293.1
MSSEKKNNDYLLEVEHLKQYFPVHQGFSTIPLKAVDDISFAIRPGETLGLVGESGCGKTT
VGRTLLRLYQPTAGKITFDGKVLFDSGEQYDENGKMIVDANGKPVMGKKVDVNMMPYRKE
MQMVFQDPYSSLNPRMTVEDIIGEPLDVHKLYRNRKERREKILDLMELVGLNAEHAMRYA
HEFSGGQRQRIGIARALAVNPKFIVCDEAVSALDVSIQAQVVNMFEELQEKLGVAYLFIA
HDLLVVHHISDRIAVMYLGKMMEIADADELNANPIHPYTLSLLSAVPIPDPETARKSHRI
VLEGDVPSPLKMPTGCPFRTRCKYATEKCGQEMPQLTDRGNGHMVACWNK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory