SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_055434897.1 NCBI__GCF_001418085.1:WP_055434897.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6lpiB Crystal structure of ahas holo-enzyme (see paper)
43% identity, 97% coverage: 15:574/577 of query aligns to 2:538/539 of 6lpiB

query
sites
6lpiB

T

T

T

R

E

K

R

R

I

F

S

T

G

G

S

A

E

E

A

F

V

I

I

V

R

H

C

F

L

L

L

E

A

Q

E

Q

G

G

V

I

D

K

I

I

L

V

Y

T

G

G

Y
x
I

P

P

G
|
G

G
|
G

A
x
S

I
|
I

M

L

P

P

V

V

Y

Y

D

D

E

A

L

L

Y

S

K

Q

Y

-

Q

S

D

T

K

Q

I

I

H

R

H

H

V

I

L

L

T

A

R

R

H

H

E
|
E

Q

Q

G

G

A

A

T

G

H

F

S

I

A

A

Q

Q

G

G

Y

M

A

A

R

R

I

T

S

D

G

G

K

K

V

P

G

A

V

V

A

C

I

M

A

A

T
x
C

S

S

G

G

P
|
P

G

G

A

A

T

T

N

N

L

L

I

V

T

T

G

A

I

I

A

A

D

D

A

A

Q

R

I

L

D

D

S

S

T

I

P

P

M

L

V

I

C

C

I

I

T

T

G

G

Q

Q

V

V

A

P

S

A

H

S

L

M

L

I

G

G

S

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

V

D

D

I

T

V

Y

G

G

I

I

S

S

T

I

P

P

V

I

T

T

K

K

W

H

N

N

C

Y

Q

L

V

V

T

R

K

H

A

I

S

E

Q

E

I

L

P

P

E

Q

A

V

M

M

A

S

K

D

A

A

F

F

Y

R

I

I

A

A

R

Q

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

W

I

I

D

D

I

I

T

P

K
|
K

D

D

A

V

Q

Q

F

T

E

A

E

V

F

F

D

E

F

I

K

E

Y

T

E

Q

K

P

C

A

K

M

G

A

V

E

R

K

S

A

Y

A

I

-

P

-

I

A

P

P

K

A

L

F

D

S

Q

E

T

E

S

S

V

I

E

R

A

D

A

A

A

A

K

A

L

M

I

I

N

N

A

A

A

A

K

K

K

R

P

P

M

V

I

L

V

Y

W

L

G
|
G

Q
x
G

G
|
G

V

V

I

I

L

-

G

-

E

N

A

A

E

P

A

A

E

R

F

V

K

R

A

E

V

L

V

A

E

E

K

K

A

A

G

Q

I

L

P

P

S

T

A

T

W

M

T
|
T

I
x
L

L
x
M

G

A

A

L

G

G

A

M

I

L

P

P

T

K

S

A

H

H

P

P

L

L

N

S

I

L

G

G

M

M

V
x
L

G

G

M
|
M

H
|
H

G

G

N

V

Y

R

A

S

P

T

N

N

V

Y

L

I

T

L

N

Q

D

E

C

A

D

D

V

L

L

L

I

I

A

V

I

L

G
|
G

M
x
A

R
|
R

F

F

D
|
D

D

D

R
|
R

V
x
A

T

I

G

G

S

K

L

T

S

E

T

Q

Y

F

A

C

K

P

Q

N

A

A

K

K

V

I

I

I

H

H

F

V

D
|
D

I
|
I

D

D

P

R

A

A

E

E

I

L

D

G

K

K

N

I

V

K

K

Q

T

P

D

H

V

V

A

A

V

I

L

Q

G

A

D
|
D

S
x
V

K

D

E

D

S

V

L

L

A

A

A

Q

L

L

L

I

P

P

L

L

L

V

E

E

E

A

K

Q

T

P

Y

R

P

A

E

E

W

W

H

H

Q

Q

K

L

F

V

K

A

D

D

L

L

Y

-

A

-

I

-

E

Q

Y

R

E

E

K

D

V

P

I

L

K

S

H

H

D

-

L

-

H

-

P

-

T

-

K

-

E

Y

G

G

L

L

T

-

M

-

A

-

E

-

V

-

L

I

K

N

E

A

I

V

N

A

I

A

Q

C

T

V

K

D

G

D

D

N

A

A

A

I

I

I

V

T

T

T

D

D

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

I

W

A

T

C

A

R

Q

Y

A

A

Y

N

P

F

L

N

N

K

R

T

P

R

R

S

Q

N

W

I

L

T

T

S

S

G
|
G

G

G

L

L

G
|
G

T

T

M
|
M

G

G

F

F

G

G

L

L

P

P

A

A

A

A

I

I

G

G

A

A

K

A

M

L

A

A

A

N

P

P

D

D

R

R

E

K

V

V

I

L

S

C

I

F

S

S

G
|
G

D
|
D

G
|
G

G
x
S

Y

L

Q

M

M
|
M

T

N

I

I

Q

Q

E

E

L

M

G

A

T

T

I

A

F

S

Q

E

Q

N

K

Q

A

L

G

D

V

V

K

K

I

I

V

I

V

L

L

M

N

N

N
|
N

E

E

F
x
A

L
|
L

G
|
G

M
x
L

V
|
V

R

H

Q

Q

W
x
Q

Q

Q

E

S

M

L

F

F

F

Y

D

E

R

Q

R

G

-

V

Y

F

A

A

S

A

T

T

E

Y

M

P

T

G

N

K

P

I

D

N

F

F

V

M

A

Q

I

I

A

A

K

A

G

G

Y

F

F

G

I

L

P

-

A

-

E

-

K

-

V

-

T

-

K

E

R

T

E

C

D

D

L

L

K

P

D

Q

A

A

-

S

V

L

A

Q

K

E

M

I

I

I

N

N

T

R

D

P

G

G

P

P

Y

A

F

L

L

I

E

H

V

V

C

R

V

I

E

D

K

A

E

E

G

E

K

K

V

V

F

Y

P

P

M

M

I

V

P

P

T

P

G

G

A

A

S

A

V

N

S

T

E

E

V

M

active site: I27 (≠ Y40), G29 (= G42), G30 (= G43), S31 (≠ A44), I32 (= I45), E53 (= E67), C76 (≠ T90), F115 (= F129), Q116 (= Q130), E117 (= E131), K165 (= K179), M256 (= M274), A283 (≠ V301), V375 (= V407), G401 (= G433), M403 (= M435), D428 (= D460), N455 (= N487), A457 (≠ F489), L458 (= L490), L460 (≠ M492), V461 (= V493), Q464 (≠ W496)
binding flavin-adenine dinucleotide: R155 (= R169), G212 (= G228), G213 (≠ Q229), G214 (= G230), T236 (= T254), L237 (≠ I255), M238 (≠ L256), L254 (≠ V272), M256 (= M274), H257 (= H275), G276 (= G294), A277 (≠ M295), R278 (= R296), D280 (= D298), R282 (= R300), A283 (≠ V301), D300 (= D318), I301 (= I319), D319 (= D337), V320 (≠ S338), M380 (= M412), G398 (= G430)
binding magnesium ion: D428 (= D460), N455 (= N487)
binding thiamine diphosphate: E53 (= E67), C76 (≠ T90), P79 (= P93), G376 (= G408), Q377 (= Q409), H378 (= H410), G401 (= G433), M403 (= M435), G427 (= G459), D428 (= D460), G429 (= G461), S430 (≠ G462), M433 (= M465), N455 (= N487), A457 (≠ F489), L458 (= L490), G459 (= G491), L460 (≠ M492), V461 (= V493)

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
42% identity, 100% coverage: 3:577/577 of query aligns to 76:660/667 of P09342

query
sites
P09342

T

S

K

Q

T

T

I

E

K

K

K

T

E

E

T

T

N

F

T

V

T

S

Q

R

-

F

-

A

T

P

T

D

E

E

R

P

I

R

S

K

G

G

S

S

E

D

A

V

V

L

I

V

R

E

C

A

L

L

L

E

A

R

E

E

G

G

V

V

D

T

I

D

L

V

Y

F

G

A

Y

Y

P

P

G

G

G

G

A

A

I

S

M

M

P

E

V

I

Y

H

D

Q

E

A

L

L

Y

T

K

R

Y

-

Q

S

D

S

K

I

I

I

H

R

H

N

V

V

L

L

T

P

R

R

H

H

E

E

Q

Q

G

G

A

G

T

V

H

F

S

A

A

A

Q

E

G

G

Y

Y

A

A

R

R

I

A

S

T

G

G

K

F

V

P

G

G

V

V

A
x
C

I

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

I

V

T

S

G

G

I

L

A

A

D

D

A

A

Q

L

I

L

D

D

S

S

T

V

P

P

M

I

V

V

C

A

I

I

T

T

G

G

Q

Q

V

V

A
x
P

S

R

H

R

L

M

L

I

G

G

S

T

D

D

A

A

F

F

Q

Q

E

E

T

T

D

P

I

I

V

V

G

E

I

V

S

T

T

R

P

S

V

I

T

T

K

K

W

H

N

N

C

Y

Q

L

V

V

T

M

K

D

A

V

S

E

Q

D

I

I

P

P

E

R

A

V

M

V

A

R

K

E

A

A

F

F

Y

F

I

L

A

A

R

R

S

S

G

G

R

R

P

P

G

G

P

P

V

I

L

L

I

I

D

D

I

V

T

P

K

K

D

D

A

I

Q

Q

F

Q

E

Q

E

L

F

V

D

I

F

P

K

D

Y

W

E

D

K

Q

C

P

K

M

G

R

V

L

R

P

S

G

Y

Y

I

M

P

S

-

R

I

L

P

P

K

K

L

L

-

P

D

N

Q

E

T

M

S

L

V

L

E

E

A

Q

A

I

A

V

K

R

L

L

I

I

N

S

A

E

A

S

K

K

K

K

P

P

M

V

I

L

V

Y

W

V

G

G

Q

G

G

G

V
x
C

I

-

L

-

G

S

E

Q

A

S

E

S

A

E

E

D

F

L

K

R

A

R

V

F

V

V

E

E

K

L

A

T

G

G

I

I

P

P

S

V

A

A

W

S

T

T

I

L

L

M

G

G

A

L

G

G

A

A

I

F

P

P

T

T

S

G

H

D

P

E

L

L

N

S

I

L

G

S

M

M

V

L

G

G

M

M

H

H

G

G

N

T

Y

V

A

Y

P

A

N

N

V

Y

L

A

T

V

N

D

D

S

C

S

D

D

V

L

L

L

I

L

A

A

I

F

G

G

M

V

R

R

F

F

D

D

D

D

R

R

V

V

T

T

G

G

S

K

L

L

S

E

T

A

Y

F

A

A

K

S

Q

R

A

A

K

K

V

I

I

V

H

H

F

I

D

D

I

I

D

D

P

S

A

A

E

E

I

I

D

G

K

K

N

N

V

K

K

Q

T

P

D

H

V

V

A

S

V

I

L

C

G

A

D

D

S

I

K

K

E

L

S

A

L

L

A

Q

A

G

L

L

L

N

P

S

L

I

L

L

E

E

E

S

K

K

T

E

-

G

-

K

-

L

-

K

-

L

-

D

Y

F

P

S

E

A

W

W

H

R

Q

Q

K

E

F

L

K

T

D

-

L

-

Y

-

A

-

I

-

E

-

Y

-

E

E

K

Q

V

K

I

V

K

K

H

H

D

P

L

L

H

N

P

F

T

K

K

T

E

F

G

G

L

D

T

A

M

I

A

P

-

P

-

Q

-

Y

-

A

-

I

E

Q

V

V

L

L

K

D

E

E

I

L

N

-

I

-

Q

-

T

T

K

N

G

G

D

N

A

A

A

I

I

I

V

S

T

T

D

G

V

V

G

G

Q

Q

H

H

Q

Q

M

M

I

W

A

A

C

A

R

Q

Y

Y

A

Y

N

K

F

Y

N

R

K

K

T

P

R

R

S

Q

N

W

I

L

T

T

S

S

G

G

G

G

L

L

G

G

T

A

M

M

G

G

F

F

G

G

L

L

P

P

A

A

A

A

I

I

G

G

A

A

K

A

M

V

A

G

A

R

P

P

D

D

R

E

E

V

V

V

I

V

S

D

I

I

S

D

G

G

D

D

G

G

G

S

Y

F

Q

I

M

M

T

N

I

V

Q

Q

E

E

L

L

G

A

T

T

I

I

F

K

Q

V

Q

E

K

N

A

L

G

P

V

V

K

K

I

I

V

M

V

L

L

L

N

N

N

N

E

Q

F

H

L

L

G

G

M

M

V

V

R

V

Q

Q

W

W

Q

E

E

D

M

R

F

F

F

Y

D

K

R

A

R

N

Y

R

A

A

S

H

T

T

E

Y

M

L

T

G

N

N

-

P

-

S

-

N

-

E

-

A

-

E

-

I

-

F

P

P

D

N

F

M

V

L

A

K

I

F

A

A

K

E

G

A

Y

C

F

G

I

V

P

P

A

A

E

A

K

R

V

V

T

T

K

H

R

R

E

D

D

D

L

L

K

R

D

A

A

A

V

I

A

Q

K

K

M

M

I

L

N

D

T

T

D

P

G

G

P

P

Y

Y

F

L

L

L

E

D

V

V

C

I

V

V

E

P

K

H

E

Q

G

E

K

H

V

V

F

L

P

P

M

M

I

I

P

P

T

S

G

G

A

G

S

A

V

F

S

K

E

D

V

V

R

I

L

T

E

E

C161 (≠ A87) modified: Disulfide link with 307
P194 (≠ A120) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ V231) modified: Disulfide link with 161

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
42% identity, 100% coverage: 3:577/577 of query aligns to 73:657/664 of P09114

query
sites
P09114

T

S

K

E

T

T

I

Q

K

K

K

A

E

E

T

T

N

F

T

V

T

S

Q

R

-

F

-

A

T

P

T

D

E

E

R

P

I

R

S

K

G

G

S

S

E

D

A

V

V

L

I

V

R

E

C

A

L

L

L

E

A

R

E

E

G

G

V

V

D

T

I

D

L

V

Y

F

G

A

Y

Y

P

P

G

G

G

G

A

A

I

S

M

M

P

E

V

I

Y

H

D

Q

E

A

L

L

Y

T

K

R

Y

-

Q

S

D

S

K

I

I

I

H

R

H

N

V

V

L

L

T

P

R

R

H

H

E

E

Q

Q

G

G

A

G

T

V

H

F

S

A

A

A

Q

E

G

G

Y

Y

A

A

R

R

I

A

S

T

G

G

K

F

V

P

G

G

V

V

A

C

I

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

I

V

T

S

G

G

I

L

A

A

D

D

A

A

Q

L

I

L

D

D

S

S

T

V

P

P

M

I

V

V

C

A

I

I

T

T

G

G

Q

Q

V

V

A
x
P

S

R

H

R

L

M

L

I

G

G

S

T

D

D

A

A

F

F

Q

Q

E

E

T

T

D

P

I

I

V

V

G

E

I

V

S

T

T

R

P

S

V

I

T

T

K

K

W

H

N

N

C

Y

Q

L

V

V

T

M

K

D

A

V

S

E

Q

D

I

I

P

P

E

R

A

V

M

V

A

R

K

E

A

A

F

F

Y

F

I

L

A

A

R

R

S

S

G

G

R

R

P

P

G

G

P

P

V

V

L

L

I

I

D

D

I

V

T

P

K

K

D

D

A

I

Q

Q

F

Q

E

Q

E

L

F

V

D

I

F

P

K

D

Y

W

E

D

K

Q

C

P

K

M

G

R

V

L

R

P

S

G

Y

Y

I

M

P

S

-

R

I

L

P

P

K

K

L

L

-

P

D

N

Q

E

T

M

S

L

V

L

E

E

A

Q

A

I

A

V

K

R

L

L

I

I

N

S

A

E

A

S

K

K

K

K

P

P

M

V

I

L

V

Y

W

V

G

G

Q

G

G

G

V

C

I

-

L

-

G

S

E

Q

A

S

E

S

A

E

E

E

F

L

K

R

A

R

V

F

V

V

E

E

K

L

A

T

G

G

I

I

P

P

S

V

A

A

W

S

T

T

I

L

L

M

G

G

A

L

G

G

A

A

I

F

P

P

T

T

S

G

H

D

P

E

L

L

N

S

I

L

G

S

M

M

V

L

G

G

M

M

H

H

G

G

N

T

Y

V

A

Y

P

A

N

N

V

Y

L

A

T

V

N

D

D

S

C

S

D

D

V

L

L

L

I

L

A

A

I

F

G

G

M

V

R

R

F

F

D

D

D

D

R

R

V

V

T

T

G

G

S

K

L

L

S

E

T

A

Y

F

A

A

K

S

Q

R

A

A

K

K

V

I

I

V

H

H

F

I

D

D

I

I

D

D

P

S

A

A

E

E

I

I

D

G

K

K

N

N

V

K

K

Q

T

P

D

H

V

V

A

S

V

I

L

C

G

A

D

D

S

I

K

K

E

L

S

A

L

L

A

Q

A

G

L

L

L

N

P

S

L

I

L

L

E

E

E

S

K

K

T

E

-

G

-

K

-

L

-

K

-

L

-

D

Y

F

P

S

E

A

W

W

H

R

Q

Q

K

E

F

L

-

T

-

V

-

Q

K

K

D

V

L

K

Y

Y

A

P

I

L

E

N

Y

F

E

K

K

T

V

F

I

-

K

-

H

G

D

D

L

A

H

I

P

P

T

P

K

Q

E

Y

G

A

L

I

T

-

M

-

A

-

E

Q

V

V

L

L

K

D

E

E

I

L

N

-

I

-

Q

-

T

T

K

N

G

G

D

S

A

A

A

I

I

I

V

S

T

T

D

G

V

V

G

G

Q

Q

H

H

Q

Q

M

M

I

W

A

A

C

A

R

Q

Y

Y

A

Y

N

K

F

Y

N

R

K

K

T

P

R

R

S

Q

N

W

I

L

T

T

S

S

G

G

G

G

L

L

G

G

T

A

M

M

G

G

F

F

G

G

L

L

P

P

A

A

A

A

I

I

G

G

A

A

K

A

M

V

A

G

A

R

P

P

D

D

R

E

E

V

V

V

I

V

S

D

I

I

S

D

G

G

D

D

G

G

G

S

Y

F

Q

I

M

M

T

N

I

V

Q

Q

E

E

L

L

G

A

T

T

I

I

F

K

Q

V

Q

E

K

N

A

L

G

P

V

V

K

K

I

I

V

M

V

L

L

L

N

N

N

N

E

Q

F

H

L

L

G

G

M

M

V

V

R

V

Q

Q

W
|
W

Q

E

E

D

M

R

F

F

F

Y

D

K

R

A

R

N

Y

R

A

A

S

H

T

T

E

Y

M

L

T

G

N

N

-

P

-

S

-

N

-

E

-

A

-

E

-

I

-

F

P

P

D

N

F

M

V

L

A

K

I

F

A

A

K

E

G

A

Y

C

F

G

I

V

P

P

A

A

E

A

K

R

V

V

T

T

K

H

R

R

E

D

D

D

L

L

K

R

D

A

A

A

V

I

A

Q

K

K

M

M

I

L

N

D

T

T

D

P

G

G

P

P

Y

Y

F

L

L

L

E

D

V

V

C

I

V

V

E

P

K

H

E

Q

G

E

K

H

V

V

F

L

P

P

M

M

I

I

P

P

T

S

G

G

A

G

S

A

V

F

S

K

E

D

V

V

R

I

L

T

E

E

P191 (≠ A120) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W496) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

6demA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide bensulfuron methyl (see paper)
42% identity, 97% coverage: 19:576/577 of query aligns to 12:576/597 of 6demA

query
sites
6demA

I

L

S

T

G

G

S

G

E

E

A

I

V

F

I

H

R

E

C

M

L

M

L

L

A

R

E

H

G

K

V

V

D

D

I

T

L

V

Y

F

G

G

Y
|
Y

P

A

G
|
G

G
|
G

A
|
A

I
|
I

M

L

P

P

V

V

Y

F

D

D

E

A

L

I

Y

Y

K

N

Y

-

Q

S

D

D

K

K

I

F

H

K

H

F

V

V

L

L

T

P

R

R

H

H

E
|
E

Q

Q

G

G

A

A

T

G

H

H

S

M

A

A

Q

E

G

G

Y

Y

A

A

R

R

I

A

S

S

G

G

K

K

V

P

G

G

V

V

A

V

I

L

A

V

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

V

I

I

T

T

G

P

I

M

A

A

D

D

A

A

Q

L

I

M

D

D

S

G

T

V

P

P

M

L

V

V

C

V

I

F

T

S

G

G

Q

Q

V

V

A

P

S

T

H

T

L

A

L

I

G

G

S

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

A

D

D

I

I

V

V

G

G

I

I

S

S

T

R

P

S

V

C

T

T

K

K

W

W

N

N

C

V

Q

M

V

V

T

K

K

N

A

V

S

A

Q

E

I

L

P

P

E

R

A

R

M

I

A

N

K

E

A

A

F

F

Y

E

I

I

A

A

R

T

S

T

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

I

V

D

D

I

L

T

P

K
|
K

D

D

A

V

Q

-

F

-

E

-

E

-

F

-

D

-

F

-

K

-

Y

-

E

-

K

T

C

A

K

S

G

I

V

L

R

R

S

E

Y

S

I

I

P

P

I

I

-

N

-

T

-

T

-

L

P

P

K

S

L

V

D

S

Q

E

T

F

S

T

V

S

E

E

A

A

-

I

-

K

-

R

A

A

A

A

K

N

L

I

I

L

N

N

A

K

A

A

K

K

K

K

P

P

M

I

I

I

V

Y

W

A

G
|
G

Q
x
A

G
|
G

V

I

I

L

L
x
N

G

N

E

E

A

Q

E

G

A

P

E
x
K

F

L

-

L

K

K

A

E

V

L

V

A

E

D

K

K

A

A

G

N

I

I

P

P

S

V

A

T

W

T

T
|
T

I
x
L

L
x
Q

G

G

A

L

G

G

A

A

I

F

P

D

T

Q

S

R

H

D

P

P

L

K

N

S

I

L

G

D

M

M

V
x
L

G

G

M
|
M

H

H

G

G

N

S

Y

A

A

A

P

A

N

N

V

T

L

A

T

I

N

Q

D

N

C

A

D

D

V

C

L

I

I

I

A

A

I

L

G
|
G

M
x
A

R
|
R

F

F

D
|
D

D
|
D

R
|
R

V
|
V

T

T

G

G

S

N

L

I

S

S

T

K

Y

F

A

A

K

P

Q

E

A

A

K

K

-

L

-

A

-

A

-

S

-

E

-

G

-

R

-

G

-

G

V

I

I

L

H

H

F

F

D
x
E

I
|
I

D

S

P

P

A

K

E
x
N

I

I

D

N

K

K

N

V

V

V

K

E

T

A

D

T

V

E

A

A

V

I

L

E

G

G

D
|
D

S
x
V

K

T

E

A

S

N

L

L

A

Q

A

S

L

F

L

I

P

P

L

L

L

V

E

D

E

S

-

I

K

E

T

N

Y

R

P

P

E

E

W

W

H

F

Q

N

K

K

-

I

-

N

-

E

F

W

K

K

D

K

L

K

Y

Y

A

P

I

Y

E

S

Y

Y

E

Q

K

-

V

-

I

-

K

-

H

-

D

-

L

L

H

E

P

T

T

P

K

G

E

S

G

L

L

I

T

K

M

P

A

Q

E

T

V

L

L

I

K

K

E

E

I

I

N

S

I

D

Q

Q

T

A

K

Q

-

T

-

Y

-

N

G

K

D

E

A

V

A

I

I

V

V

T

T

T

D

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

I

W

A

A

C

A

R

Q

Y

H

A

F

N

T

F

W

N

T

K

Q

T

P

R

R

S

T

N

M

I

I

T

T

S

S

G
|
G

G

G

L
|
L

G
|
G

T

T

M
|
M

G

G

F

Y

G

G

L

L

P

P

A

A

A

A

I

I

G

G

A

A

K

Q

M

V

A

A

A

K

P

P

D

D

R

A

E

I

V

V

I

I

S

D

I

I

S

D

G
|
G

D
|
D

G
x
A

G
x
S

Y

F

Q

N

M
|
M

T

T

I

L

Q

T

E

E

L

L

G

S

T

S

I

A

F

V

Q

Q

Q

A

K

G

A

A

G

P

V

I

K

K

I

V

V

C

V

V

L

L

N

N

N
|
N

E

E

F
x
E

L
x
Q

G
|
G

M
|
M

V
|
V

R

T

Q

Q

W
|
W

Q

Q

E

S

M

L

F

F

F

Y

D

E

R

H

R

R

Y

Y

A

S

S

H

T

T

E

H

M

Q

T

S

N

N

P

P

D

D

F

F

V

M

A

K

I
x
L

A

A

K

E

G

S

Y

M

F
x
N

I
x
V

P

K

A

G

E

I

K

R

V

I

T

T

K

N

R

Q

E

Q

D

E

L

L

K

K

D

S

A

G

V

V

A

K

K

E

M

F

I

L

N

D

T

A

D

T

G

E

P

P

Y

V

F

L

L

L

E

E

V

V

C

I

V

V

E

E

K

K

E

K

G

V

K

P

V

V

F

L

P

P

M

M

I

V

P

P

T
x
A

G

G

A

K

S

A

V

L

S

D

E

D

V

F

R

I

L

L

active site: Y33 (= Y40), G35 (= G42), G36 (= G43), A37 (= A44), I38 (= I45), E59 (= E67), T82 (= T90), F121 (= F129), Q122 (= Q130), E123 (= E131), K171 (= K179), K228 (≠ E239), M264 (= M274), V291 (= V301), V407 (= V407), L432 (= L432), G433 (= G433), M435 (= M435), D460 (= D460), N487 (= N487), E489 (≠ F489), Q490 (≠ L490), M492 (= M492), V493 (= V493), W496 (= W496), L518 (≠ I518), N523 (≠ F523), V524 (≠ I524)
binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: M264 (= M274), D289 (= D299), R290 (= R300), M492 (= M492), W496 (= W496), A567 (≠ T567)
binding flavin-adenine dinucleotide: R161 (= R169), G217 (= G228), A218 (≠ Q229), G219 (= G230), N222 (≠ L233), T244 (= T254), L245 (≠ I255), Q246 (≠ L256), L262 (≠ V272), G284 (= G294), A285 (≠ M295), R286 (= R296), D288 (= D298), R290 (= R300), V291 (= V301), E317 (≠ D318), I318 (= I319), N322 (≠ E323), D336 (= D337), V337 (≠ S338), M412 (= M412), G430 (= G430)
binding magnesium ion: D460 (= D460), N487 (= N487), E489 (≠ F489)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (= V407), G408 (= G408), Q409 (= Q409), H410 (= H410), M435 (= M435), G459 (= G459), D460 (= D460), A461 (≠ G461), S462 (≠ G462), M465 (= M465), N487 (= N487), E489 (≠ F489), Q490 (≠ L490), G491 (= G491), M492 (= M492), V493 (= V493)

6delA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide chlorimuron ethyl (see paper)
42% identity, 97% coverage: 19:576/577 of query aligns to 12:576/597 of 6delA

query
sites
6delA

I

L

S

T

G

G

S

G

E

E

A

I

V

F

I

H

R

E

C

M

L

M

L

L

A

R

E

H

G

K

V

V

D

D

I

T

L

V

Y

F

G

G

Y
|
Y

P

A

G
|
G

G
|
G

A
|
A

I
|
I

M

L

P

P

V

V

Y

F

D

D

E

A

L

I

Y

Y

K

N

Y

-

Q

S

D

D

K

K

I

F

H

K

H

F

V

V

L

L

T

P

R

R

H

H

E
|
E

Q

Q

G

G

A

A

T

G

H

H

S

M

A

A

Q

E

G

G

Y

Y

A

A

R

R

I

A

S

S

G

G

K

K

V

P

G

G

V

V

A

V

I

L

A

V

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

V

I

I

T

T

G

P

I

M

A

A

D

D

A

A

Q

L

I

M

D

D

S

G

T

V

P

P

M

L

V

V

C

V

I

F

T

S

G

G

Q

Q

V

V

A

P

S

T

H

T

L

A

L

I

G

G

S

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

A

D

D

I

I

V

V

G

G

I

I

S

S

T

R

P

S

V

C

T

T

K

K

W

W

N

N

C

V

Q

M

V

V

T

K

K

N

A

V

S

A

Q

E

I

L

P

P

E

R

A

R

M

I

A

N

K

E

A

A

F

F

Y

E

I

I

A

A

R

T

S

T

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

I

V

D

D

I

L

T

P

K
|
K

D

D

A

V

Q

-

F

-

E

-

E

-

F

-

D

-

F

-

K

-

Y

-

E

-

K

T

C

A

K

S

G

I

V

L

R

R

S

E

Y

S

I

I

P

P

I

I

-

N

-

T

-

T

-

L

P

P

K

S

L

V

D

S

Q

E

T

F

S

T

V

S

E

E

A

A

-

I

-

K

-

R

A

A

A

A

K

N

L

I

I

L

N

N

A

K

A

A

K

K

K

K

P

P

M

I

I

I

V

Y

W

A

G
|
G

Q
x
A

G
|
G

V

I

I

L

L
x
N

G

N

E

E

A

Q

E

G

A

P

E
x
K

F

L

-

L

K

K

A

E

V

L

V

A

E

D

K

K

A

A

G

N

I

I

P

P

S

V

A

T

W

T

T
|
T

I
x
L

L
x
Q

G

G

A

L

G

G

A

A

I

F

P

D

T

Q

S

R

H

D

P

P

L

K

N

S

I

L

G

D

M

M

V
x
L

G

G

M
|
M

H

H

G

G

N

S

Y

A

A

A

P

A

N

N

V

T

L

A

T

I

N

Q

D

N

C

A

D

D

V

C

L

I

I

I

A

A

I

L

G
|
G

M
x
A

R
|
R

F

F

D
|
D

D
|
D

R
|
R

V
|
V

T

T

G

G

S

N

L

I

S

S

T

K

Y

F

A

A

K

P

Q

E

A

A

K

K

-

L

-

A

-

A

-

S

-

E

-

G

-

R

-

G

-

G

V

I

I

L

H

H

F

F

D
x
E

I
|
I

D

S

P

P

A

K

E
x
N

I

I

D

N

K

K

N

V

V

V

K

E

T

A

D

T

V

E

A

A

V

I

L

E

G

G

D
|
D

S
x
V

K

T

E

A

S

N

L

L

A

Q

A

S

L

F

L

I

P

P

L

L

L

V

E

D

E

S

-

I

K

E

T

N

Y

R

P

P

E

E

W

W

H

F

Q

N

K

K

-

I

-

N

-

E

F

W

K

K

D

K

L

K

Y

Y

A

P

I

Y

E

S

Y

Y

E

Q

K

-

V

-

I

-

K

-

H

-

D

-

L

L

H

E

P

T

T

P

K

G

E

S

G

L

L

I

T

K

M

P

A

Q

E

T

V

L

L

I

K

K

E

E

I

I

N

S

I

D

Q

Q

T

A

K

Q

-

T

-

Y

-

N

G

K

D

E

A

V

A

I

I

V

V

T

T

T

D

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

I

W

A

A

C

A

R

Q

Y

H

A

F

N

T

F

W

N

T

K

Q

T

P

R

R

S

T

N

M

I

I

T

T

S

S

G
|
G

G

G

L
|
L

G
|
G

T

T

M
|
M

G

G

F

Y

G

G

L

L

P

P

A

A

A

A

I

I

G

G

A

A

K

Q

M

V

A

A

A

K

P

P

D

D

R

A

E

I

V

V

I

I

S

D

I

I

S

D

G
|
G

D
|
D

G
x
A

G
x
S

Y

F

Q

N

M
|
M

T

T

I

L

Q

T

E

E

L

L

G

S

T

S

I

A

F

V

Q

Q

Q

A

K

G

A

A

G

P

V

I

K

K

I

V

V

C

V

V

L

L

N

N

N
|
N

E

E

F
x
E

L
x
Q

G
|
G

M
|
M

V
|
V

R

T

Q

Q

W
|
W

Q

Q

E

S

M

L

F

F

F

Y

D

E

R

H

R

R

Y

Y

A

S

S

H

T

T

E

H

M

Q

T

S

N

N

P

P

D

D

F

F

V

M

A

K

I
x
L

A

A

K

E

G

S

Y

M

F
x
N

I
x
V

P

K

A

G

E

I

K

R

V

I

T

T

K

N

R

Q

E

Q

D

E

L

L

K

K

D

S

A

G

V

V

A

K

K

E

M

F

I

L

N

D

T

A

D

T

G

E

P

P

Y

V

F

L

L

L

E

E

V

V

C

I

V

V

E

E

K

K

E

K

G

V

K

P

V

V

F

L

P

P

M

M

I

V

P

P

T

A

G

G

A

K

S

A

V

L

S

D

E

D

V

F

R

I

L

L

active site: Y33 (= Y40), G35 (= G42), G36 (= G43), A37 (= A44), I38 (= I45), E59 (= E67), T82 (= T90), F121 (= F129), Q122 (= Q130), E123 (= E131), K171 (= K179), K228 (≠ E239), M264 (= M274), V291 (= V301), V407 (= V407), L432 (= L432), G433 (= G433), M435 (= M435), D460 (= D460), N487 (= N487), E489 (≠ F489), Q490 (≠ L490), M492 (= M492), V493 (= V493), W496 (= W496), L518 (≠ I518), N523 (≠ F523), V524 (≠ I524)
binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: D289 (= D299), R290 (= R300), W496 (= W496)
binding flavin-adenine dinucleotide: R161 (= R169), G217 (= G228), A218 (≠ Q229), G219 (= G230), N222 (≠ L233), T244 (= T254), L245 (≠ I255), Q246 (≠ L256), L262 (≠ V272), G284 (= G294), A285 (≠ M295), R286 (= R296), D288 (= D298), R290 (= R300), V291 (= V301), E317 (≠ D318), I318 (= I319), N322 (≠ E323), D336 (= D337), V337 (≠ S338), M412 (= M412), G430 (= G430)
binding (3Z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl](formyl)amino}-3-sulfanylpent-3-en-1-yl trihydrogen diphosphate: V407 (= V407), G408 (= G408), Q409 (= Q409), H410 (= H410), G433 (= G433), M435 (= M435), G459 (= G459), D460 (= D460), A461 (≠ G461), S462 (≠ G462), M465 (= M465), N487 (= N487), E489 (≠ F489), Q490 (≠ L490), G491 (= G491), M492 (= M492), V493 (= V493)
binding magnesium ion: D460 (= D460), N487 (= N487), E489 (≠ F489)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (= V407), G408 (= G408), Q409 (= Q409), H410 (= H410), G433 (= G433), M435 (= M435), G459 (= G459), D460 (= D460), A461 (≠ G461), S462 (≠ G462), M465 (= M465), N487 (= N487), E489 (≠ F489), Q490 (≠ L490), G491 (= G491), M492 (= M492), V493 (= V493)

6denA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron ethyl (see paper)
41% identity, 99% coverage: 7:576/577 of query aligns to 2:578/599 of 6denA

query
sites
6denA

K

K

K

K

E

E

T

Q

N

L

T

M

T

D

Q

D

T

S

T

F

E

I

R

G

I

L

S

T

G

G

S

G

E

E

A

I

V

F

I

H

R

E

C

M

L

M

L

L

A

R

E

H

G

K

V

V

D

D

I

T

L

V

Y

F

G

G

Y
|
Y

P

A

G
|
G

G
|
G

A
|
A

I
|
I

M

L

P

P

V

V

Y

F

D

D

E

A

L

I

Y

Y

K

N

Y

-

Q

S

D

D

K

K

I

F

H

K

H

F

V

V

L

L

T

P

R

R

H

H

E
|
E

Q

Q

G

G

A

A

T

G

H

H

S

M

A

A

Q

E

G

G

Y

Y

A

A

R

R

I

A

S

S

G

G

K

K

V

P

G

G

V

V

A

V

I

L

A

V

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

V

I

I

T

T

G

P

I

M

A

A

D

D

A

A

Q

L

I

M

D

D

S

G

T

V

P

P

M

L

V

V

C

V

I

F

T

S

G

G

Q

Q

V

V

A

P

S

T

H

T

L

A

L

I

G

G

S

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

A

D

D

I

I

V

V

G

G

I

I

S

S

T

R

P

S

V

C

T

T

K

K

W

W

N

N

C

V

Q

M

V

V

T

K

K

N

A

V

S

A

Q

E

I

L

P

P

E

R

A

R

M

I

A

N

K

E

A

A

F

F

Y

E

I

I

A

A

R

T

S

T

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

I

V

D

D

I

L

T

P

K
|
K

D

D

A

V

Q

-

F

-

E

-

E

-

F

-

D

-

F

-

K

-

Y

-

E

-

K

T

C

A

K

S

G

I

V

L

R

R

S

E

Y

S

I

I

P

P

I

I

-

N

-

T

-

T

-

L

P

P

K

A

L

V

D

S

Q

E

T

F

S

T

V

S

E

E

A

A

-

I

-

K

-

R

A

A

A

A

K

N

L

I

I

L

N

N

A

K

A

A

K

K

K

K

P

P

M

I

I

I

V

Y

W

A

G
|
G

Q
x
A

G
|
G

V

I

I

L

L
x
N

G

N

E

E

A

Q

E

G

A

P

E
x
K

F

L

-

L

K

K

A

E

V

L

V

A

E

D

K

K

A

A

G

N

I

I

P

P

S

V

A

T

W

T

T
|
T

I
x
L

L
x
Q

G

G

A

L

G

G

A

A

I

F

P

D

T

Q

S

R

H

D

P

P

L

K

N

S

I

L

G

D

M

M

V
x
L

G

G

M
|
M

H

H

G

G

N

S

Y

A

A

A

P

A

N

N

V

T

L

A

T

I

N

Q

D

N

C

A

D

D

V

C

L

I

I

I

A

A

I

L

G
|
G

M
x
A

R
|
R

F

F

D
|
D

D
|
D

R
|
R

V
|
V

T

T

G

G

S

N

L

I

S

S

T

K

Y

F

A

A

K

P

Q

E

A

A

K

K

-

L

-

A

-

A

-

S

-

E

-

G

-

R

-

G

-

G

V

I

I

L

H

H

F

F

D
x
E

I
|
I

D

S

P

P

A

K

E
x
N

I

I

D

N

K

K

N

V

V

V

K

E

T

A

D

T

V

E

A

A

V

I

L

E

G

G

D
|
D

S
x
V

K

T

E

A

S

N

L

L

A

Q

A

S

L

F

L

I

P

P

L

L

L

V

E

D

E

S

-

I

K

E

T

N

Y

R

P

P

E

E

W

W

H

F

Q

N

K

K

-

I

-

N

-

E

F

W

K

K

D

K

L

K

Y

Y

A

P

I

Y

E

S

Y

Y

E

Q

K

-

V

-

I

-

K

-

H

-

D

-

L

L

H

E

P

T

T

P

K

G

E

S

G

L

L

I

T

K

M

P

A

Q

E

T

V

L

L

I

K

K

E

E

I

I

N

S

I

D

Q

Q

T

A

K

Q

-

T

-

Y

-

N

G

K

D

E

A

V

A

I

I

V

V

T

T

T

D

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

I

W

A

A

C

A

R

Q

Y

H

A

F

N

T

F

W

N

T

K

Q

T

P

R

R

S

T

N

M

I

I

T

T

S

S

G
|
G

G

G

L
|
L

G
|
G

T

T

M
|
M

G

G

F

Y

G

G

L

L

P

P

A

A

A

A

I

I

G

G

A

A

K

Q

M

V

A

A

A

K

P

P

D

D

R

A

E

I

V

V

I

I

S

D

I

I

S

D

G
|
G

D
|
D

G
x
A

G
x
S

Y

F

Q

N

M

M

T

T

I

L

Q

T

E

E

L

L

G

S

T

S

I

A

F

V

Q

Q

Q

A

K

G

A

A

G

P

V

I

K

K

I

V

V

C

V

V

L

L

N

N

N
|
N

E

E

F
x
E

L
x
Q

G
|
G

M
|
M

V
|
V

R

T

Q

Q

W
|
W

Q

Q

E

S

M

L

F

F

F

Y

D

E

R

H

R

R

Y

Y

A

S

S

H

T

T

E

H

M

Q

T

S

N

N

P

P

D

D

F

F

V

M

A

K

I
x
L

A

A

K

E

G

S

Y

M

F
x
N

I
x
V

P

K

A

G

E

I

K

R

V

I

T

T

K

N

R

Q

E

Q

D

E

L

L

K

K

D

S

A

G

V

V

A

K

K

E

M

F

I

L

N

D

T

A

D

T

G

E

P

P

Y

V

F

L

L

L

E

E

V

V

C

I

V

V

E

E

K

K

E

K

G

V

K

P

V

V

F

L

P

P

M

M

I

V

P

P

T

A

G

G

A

K

S

A

V

L

S

D

E

D

V

F

R

I

L

L

active site: Y35 (= Y40), G37 (= G42), G38 (= G43), A39 (= A44), I40 (= I45), E61 (= E67), T84 (= T90), F123 (= F129), Q124 (= Q130), E125 (= E131), K173 (= K179), K230 (≠ E239), M266 (= M274), V293 (= V301), V409 (= V407), L434 (= L432), G435 (= G433), M437 (= M435), D462 (= D460), N489 (= N487), E491 (≠ F489), Q492 (≠ L490), M494 (= M492), V495 (= V493), W498 (= W496), L520 (≠ I518), N525 (≠ F523), V526 (≠ I524)
binding flavin-adenine dinucleotide: R163 (= R169), G219 (= G228), A220 (≠ Q229), G221 (= G230), N224 (≠ L233), T246 (= T254), L247 (≠ I255), Q248 (≠ L256), L264 (≠ V272), G286 (= G294), A287 (≠ M295), R288 (= R296), D290 (= D298), R292 (= R300), V293 (= V301), E319 (≠ D318), I320 (= I319), N324 (≠ E323), D338 (= D337), V339 (≠ S338), M414 (= M412), G432 (= G430)
binding ethyl 2-{[(4-iodo-6-methoxypyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: M266 (= M274), D291 (= D299), R292 (= R300), W498 (= W496)
binding magnesium ion: D462 (= D460), N489 (= N487), E491 (≠ F489)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V409 (= V407), G410 (= G408), Q411 (= Q409), H412 (= H410), G435 (= G433), M437 (= M435), G461 (= G459), D462 (= D460), A463 (≠ G461), S464 (≠ G462), N489 (= N487), E491 (≠ F489), Q492 (≠ L490), G493 (= G491), M494 (= M492), V495 (= V493)

6deoA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron methyl (see paper)
41% identity, 97% coverage: 19:576/577 of query aligns to 10:572/593 of 6deoA

query
sites
6deoA

I

L

S

T

G

G

S

G

E

E

A

I

V

F

I

H

R

E

C

M

L

M

L

L

A

R

E

H

G

K

V

V

D

D

I

T

L

V

Y

F

G

G

Y
|
Y

P

A

G
|
G

G
|
G

A
|
A

I
|
I

M

L

P

P

V

V

Y

F

D

D

E

A

L

I

Y

Y

K

N

Y

-

Q

S

D

D

K

K

I

F

H

K

H

F

V

V

L

L

T

P

R

R

H

H

E
|
E

Q

Q

G

G

A

A

T

G

H

H

S

M

A

A

Q

E

G

G

Y

Y

A

A

R

R

I

A

S

S

G

G

K

K

V

P

G

G

V

V

A

V

I

L

A

V

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

V

I

I

T

T

G

P

I

M

A

A

D

D

A

A

Q

L

I

M

D

D

S

G

T

V

P

P

M

L

V

V

C

V

I

F

T

S

G

G

Q

Q

V

V

A

P

S

T

H

T

L

A

L

I

G

G

S

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

A

D

D

I

I

V

V

G

G

I

I

S

S

T

R

P

S

V

C

T

T

K

K

W

W

N

N

C

V

Q

M

V

V

T

K

K

N

A

V

S

A

Q

E

I

L

P

P

E

R

A

R

M

I

A

N

K

E

A

A

F

F

Y

E

I

I

A

A

R

T

S

T

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

I

V

D

D

I

L

T

P

K
|
K

D

D

A

V

Q

-

F

-

E

-

E

-

F

-

D

-

F

-

K

-

Y

-

E

-

K

T

C

A

K

S

G

I

V

L

R

R

S

E

Y

S

I

I

P

P

I

I

-

N

-

T

-

T

-

L

-

P

P

S

K

E

L

F

D

T

Q

S

T

E

S

A

V

I

E

K

A

R

A

A

A

A

K

N

L

I

I

L

N

N

A

K

A

A

K

K

K

K

P

P

M

I

I

I

V

Y

W

A

G
|
G

Q
x
A

G
|
G

V

I

I

L

L
x
N

G

N

E

E

A

Q

E

G

A

P

E
x
K

F

L

-

L

K

K

A

E

V

L

V

A

E

D

K

K

A

A

G

N

I

I

P

P

S

V

A

T

W

T

T
|
T

I
x
L

L
x
Q

G

G

A

L

G

G

A

A

I

F

P

D

T

Q

S

R

H

D

P

P

L

K

N

S

I

L

G

D

M

M

V
x
L

G

G

M
|
M

H

H

G

G

N

S

Y

A

A

A

P

A

N

N

V

T

L

A

T

I

N

Q

D

N

C

A

D

D

V

C

L

I

I

I

A

A

I

L

G
|
G

M
x
A

R
|
R

F

F

D
|
D

D
|
D

R
|
R

V
|
V

T

T

G

G

S

N

L

I

S

S

T

K

Y

F

A

A

K

P

Q

E

A

A

K

K

-

L

-

A

-

A

-

S

-

E

-

G

-

R

-

G

-

G

V

I

I

L

H

H

F

F

D
x
E

I
|
I

D

S

P

P

A

K

E
x
N

I

I

D

N

K

K

N

V

V

V

K

E

T

A

D

T

V

E

A

A

V

I

L

E

G

G

D
|
D

S
x
V

K

T

E

A

S

N

L

L

A

Q

A

S

L

F

L

I

P

P

L

L

L

V

E

D

E

S

-

I

K

E

T

N

Y

R

P

P

E

E

W

W

H

F

Q

N

K

K

-

I

-

N

-

E

F

W

K

K

D

K

L

K

Y

Y

A

P

I

Y

E

S

Y

Y

E

Q

K

-

V

-

I

-

K

-

H

-

D

-

L

L

H

E

P

T

T

P

K

G

E

S

G

L

L

I

T

K

M

P

A

Q

E

T

V

L

L

I

K

K

E

E

I

I

N

S

I

D

Q

Q

T

A

K

Q

-

T

-

Y

-

N

G

K

D

E

A

V

A

I

I

V

V

T

T

T

D

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

I

W

A

A

C

A

R

Q

Y

H

A

F

N

T

F

W

N

T

K

Q

T

P

R

R

S

T

N

M

I

I

T

T

S

S

G
|
G

G

G

L
|
L

G
|
G

T

T

M
|
M

G

G

F

Y

G

G

L

L

P

P

A

A

A

A

I

I

G

G

A

A

K

Q

M

V

A

A

A

K

P

P

D

D

R

A

E

I

V

V

I

I

S

D

I

I

S

D

G
|
G

D
|
D

G
x
A

G
x
S

Y

F

Q

N

M
|
M

T

T

I

L

Q

T

E

E

L

L

G

S

T

S

I

A

F

V

Q

Q

Q

A

K

G

A

A

G

P

V

I

K

K

I

V

V

C

V

V

L

L

N

N

N
|
N

E

E

F
x
E

L
x
Q

G
|
G

M
|
M

V
|
V

R

T

Q

Q

W
|
W

Q

Q

E

S

M

L

F

F

F

Y

D

E

R

H

R

R

Y

Y

A

S

S

H

T

T

E

H

M

Q

T

S

N

N

P

P

D

D

F

F

V

M

A

K

I
x
L

A

A

K

E

G

S

Y

M

F
x
N

I
x
V

P

K

A

G

E

I

K

R

V

I

T

T

K

N

R

Q

E

Q

D

E

L

L

K

K

D

S

A

G

V

V

A

K

K

E

M

F

I

L

N

D

T

A

D