SitesBLAST

Q5SLR3 2-oxoisovalerate dehydrogenase subunit beta; Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDH E1-beta; EC 1.2.4.4 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
34% identity, 33% coverage: 475:735/803 of query aligns to 10:268/324 of Q5SLR3

query
sites
Q5SLR3

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E29 (= E494) binding thiamine diphosphate
Q82 (= Q547) binding thiamine diphosphate

P21953 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial; Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDE1B; BCKDH E1-beta; EC 1.2.4.4 from Homo sapiens (Human) (see 2 papers)
32% identity, 33% coverage: 479:746/803 of query aligns to 81:348/392 of P21953

query
sites
P21953

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Y152 (= Y551) binding thiamine diphosphate
N176 (≠ Q574) to Y: in MSUD1B; uncertain significance; loss of 3-methyl-2-oxobutanoate dehydrogenase activity
G178 (≠ A576) binding K(+)
L180 (= L578) binding K(+)
T181 (≠ I579) binding K(+)
H206 (≠ L603) to R: in MSUD1B; loss of 3-methyl-2-oxobutanoate dehydrogenase activity
C228 (≠ L625) binding K(+)
D231 (≠ S628) binding K(+)
N233 (≠ E630) binding K(+)

2j9fD Human branched-chain alpha-ketoacid dehydrogenase-decarboxylase e1b (see paper)
32% identity, 33% coverage: 479:746/803 of query aligns to 18:285/329 of 2j9fD

query
sites
2j9fD

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active site: E63 (= E525), H133 (= H592)
binding c2-1-hydroxy-3-methyl-propyl-thiamin diphosphate: E33 (= E494), L61 (≠ I523), E63 (= E525), Q85 (= Q547), Y89 (= Y551), H133 (= H592)

1dtwB Human branched-chain alpha-keto acid dehydrogenase (see paper)
32% identity, 33% coverage: 479:746/803 of query aligns to 15:282/326 of 1dtwB

query
sites
1dtwB

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active site: E60 (= E525), H130 (= H592)
binding potassium ion: G112 (≠ A576), S113 (≠ P577), L114 (= L578), T115 (≠ I579), C162 (≠ L625), I163 (≠ L626), D165 (≠ S628), N167 (≠ E630), P168 (= P631), C169 (≠ A632)

P11177 Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; PDHE1-B; EC 1.2.4.1 from Homo sapiens (Human) (see 8 papers)
26% identity, 42% coverage: 448:787/803 of query aligns to 26:353/359 of P11177

query
sites
P11177

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K

P

A

V

P

P

L
x
I

I

V

V

F

R

R

T

G

R

P

G

N

H

G

R

A

L

S

E

A

G

G

I

V

W

A

H

A

S

Q

G

H

S

S

Q

Q

-

C

M

F

G

A

V

W

L

Y

N

G

L

H

I

C

R

P

G

G

I

L

H

K

V

V

L

V

V

S

P

P

R

W

N

N

M

S

T

E

K

D

A

A

A

K

G

G

F

L

Y

I

N

K

T

S

L
x
A

L
x
I

E

R

S
x
D

D

N

E
x
N

P

P

A

V

L

V

V

V

V

L

-

E

-

N

E

E

C

L

L

M

N

Y

G

G

Y

V

R

P

L

F

K

E

E

F

K

P

P

E

T

A

N

Q

F

S

G

K

E

D

F

F

K

L

T

I

P

P

I

I

G

G

V

K

V

A

E

K

T

I

V

E

K

R

E

Q

G

G

N

T

D

H

I

I

T

T

V

V

L

V

S

S

Y

H

G

S

S

R

T

P

L

V

R

G

I

H

V

C

Q

L

D

E

V

A

A

A

K

A

E

V

L

L

L

S

Q

K

V
x
E

G

G

I

V

D
x
E

V

C

E
|
E

V

V

I

I

D

N

A

M

Q

R

S

T

L

I

L

R

P

P

F

M

D

D

I

M

N

-

H

E

D

T

V

I

V

E

K

A

S

S

L

V

A

M

K

K

T

T

N

N

R

H

L

L

M

V

I

T

I

V

D

E

E

G

D

G

V

W

P

P

G

Q

-

F

G

G

A

V

S

G

A

A

Y

E

L

I

L

C

D

A

K

R

I

I

L

M

N

-

E

E

Q

G

N

P

G

A

Y

F

Q

N

Y

F

L

L

D
|
D

S

A

Q

-

P

P

K

A

T

V

L

R

A

V

A

T

K

G

A

A

H

D

R

V

P

P

-

M

A

P

Y

Y

G

A

T

K

D

I

G

L

D

E

Y

D

F

N

S

S

K

I

P

P

S

Q

A

V

E

K

D

D

I

I

Y

I

E

F

A

A

I

I

L31 (≠ Q453) natural variant: L -> V
E89 (= E525) binding thiamine diphosphate
I142 (≠ L578) binding K(+)
A190 (≠ L625) binding K(+)
I191 (≠ L626) binding K(+)
D193 (≠ S628) binding K(+)
N195 (≠ E630) binding K(+)
E259 (≠ V692) mutation E->A,Q: Does not affect interaction with DLAT.
E262 (≠ D695) mutation E->A,Q: Does not affect interaction with DLAT.
E264 (= E697) mutation E->A,Q: Does not affect interaction with DLAT.
D319 (= D753) Important for interaction with DLAT; mutation to A: Inhibits interaction with DLAT. Does not affect pyruvate decarboxylase activity. Loss of multienzyme pyruvate dehydrogenase complex activity.; mutation to N: Reduces interaction with DLAT. Reduces multienzyme pyruvate dehydrogenase complex activity. Does not affect pyruvate decarboxylase activity.

Sites not aligning to the query:

1:30 modified: transit peptide, Mitochondrion
359 I→A: Reduces pyruvate decarboxylase and multienzyme pyruvate dehydrogenase complex activity. Does not affect interaction with DLAT.; mutation Missing: Reduces pyruvate decarboxylase and multienzyme pyruvate dehydrogenase complex activity. Does not affect interaction with DLAT.

6cfoB Human pyruvate dehydrogenase e1 component complex with covalent tdp adduct acetyl phosphinate (see paper)
27% identity, 37% coverage: 490:787/803 of query aligns to 25:324/330 of 6cfoB

query
sites
6cfoB

L

F

I

L

F

L

G

G

E
|
E

D

E

A

V

G

A

A

Q

I

Y

G

D

D

G

V

A

N

Y

Q

K

G

V

L

S

E

R

G

G

L

L

Q

W

E

K

K

K

Y

Y

G

G

E

D

L

K

R

R

V

I

A

I

D

D

A

T

G

P

I
|
I

R

S

E
|
E

A

M

T

G

I

F

I

A

G

G

Q

I

G

A

I

V

G

G

M

A

A

A

M

M

R

A

G

G

L

L

R

R

P

P

I

I

A

C

E

E

I

F

Q

M

Y

T

L

F

D

N

Y
x
F

I

S

M

M

Y
x
Q

A

A

L

I

Q

D

I

Q

M

V

S

I

D

N

D

S

L

A

A

A

T

K

V

T

H

Y

Y

Y

R

M

T

S

K

G

G

G

R

L

Q

Q

K

P

A

V

P

P

L

I

I

V

V

F

R

R

T

G

R

P

G

N

H

G

R

A

L

S

E

A

G

G

I

V

W

A

H

A

S

Q

G
x
H

S

S

Q

Q

-

C

M

F

G

A

V

W

L

Y

N

G

L

H

I

C

R

P

G

G

I

L

H

K

V

V

L

V

V

S

P

P

R

W

N

N

M

S

T

E

K

D

A

A

A

K

G

G

F

L

Y

I

N

K

T

S

L

A

L

I

E

R

S

D

D

N

E

N

P

P

A

V

L

V

V

V

V

L

-

E

-

N

E

E

C

L

L

M

N

Y

G

G

Y

V

R

P

L

F

K

E

E

F

K

P

P

E

T

A

N

Q

F

S

G

K

E

D

F

F

K

L

T

I

P

P

I

I

G

G

V

K

V

A

E

K

T

I

V

E

K

R

E

Q

G

G

N

T

D

H

I

I

T

T

V

V

L

V

S

S

Y

H

G

S

S

R

T

P

L

V

R

G

I

H

V

C

Q

L

D

E

V

A

A

A

K

A

E

V

L

L

L

S

Q

K

V

E

G

G

I

V

D

E

V

C

E

E

V

V

I

I

D

N

A

M

Q

R

S

T

L

I

L

R

P

P

F

M

D

D

I

M

N

-

H

E

D

T

V

I

V

E

K

A

S

S

L

V

A

M

K

K

T

T

N

N

R

H

L

L

M

V

I

T

I

V

D

E

E

G

D

G

V

W

P

P

G

Q

-

F

G

G

A

V

S

G

A

A

Y

E

L

I

L

C

D

A

K

R

I

I

L

M

N

-

E

E

Q

G

N

P

G

A

Y

F

Q

N

Y

F

L

L

D

D

S

A

Q

-

P

P

K

A

T

V

L

R

A

V

A

T

K

G

A

A

H

D

R

V

P

P

-

M

A

P

Y

Y

G

A

T

K

D

I

G

L

D

E

Y

D

F

N

S

S

K

I

P

P

S

Q

A

V

E

K

D

D

I

I

Y

I

E

F

A

A

I

I

active site: E60 (= E525), H129 (≠ G594)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(1S)-1-hydroxy-1-[(R)-hydroxy(oxo)-lambda~5~-phosphanyl]ethyl}-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: E29 (= E494), I58 (= I523), E60 (= E525), F86 (≠ Y551), Q89 (≠ Y554)

6cerD Human pyruvate dehydrogenase complex e1 component v138m mutation (see paper)
27% identity, 37% coverage: 490:787/803 of query aligns to 26:325/331 of 6cerD

query
sites
6cerD

L

F

I

L

F

L

G

G

E

E

D

E

A

V

G

A

A

Q

I

Y

G

D

D

G

V

A

N

Y

Q

K

G

V

L

S

E

R

G

G

L

L

Q

W

E

K

K

K

Y

Y

G

G

E

D

L

K

R

R

V

I

A

I

D

D

A

T

G

P

I
|
I

R

S

E
|
E

A

M

T

G

I

F

I

A

G

G

Q

I

G

A

I

V

G

G

M

A

A

A

M

M

R

A

G

G

L

L

R

R

P

P

I

I

A

C

E

E

I

F

Q
x
M

Y

T

L

F

D

N

Y
x
F

I

S

M

M

Y

Q

A

A

L

I

Q

D

I

Q

M

V

S

I

D

N

D

S

L

A

A

A

T

K

V

T

H

Y

Y

Y

R

M

T

S

K

G

G

G

R

L

Q

Q

K

P

A

V

P

P

L

I

I

V

V

F

R

R

T

G

R

P

G

N

H

G

R

A

L

S

E

A

G

G

I

V

W

A

H

A

S

Q

G
x
H

S

S

Q

Q

-

C

M

F

G

A

V

W

L

Y

N

G

L

H

I

C

R

P

G

G

I

L

H

K

V

V

L

V

V

S

P

P

R

W

N

N

M

S

T

E

K

D

A

A

A

K

G

G

F

L

Y

I

N

K

T

S

L

A

L

I

E

R

S

D

D

N

E

N

P

P

A

V

L

V

V

V

V

L

-

E

-

N

E

E

C

L

L

M

N

Y

G

G

Y

V

R

P

L

F

K

E

E

F

K

P

P

E

T

A

N

Q

F

S

G

K

E

D

F

F

K

L

T

I

P

P

I

I

G

G

V

K

V

A

E

K

T

I

V

E

K

R

E

Q

G

G

N

T

D

H

I

I

T

T

V

V

L

V

S

S

Y

H

G

S

S

R

T

P

L

V

R

G

I

H

V

C

Q

L

D

E

V

A

A

A

K

A

E

V

L

L

L

S

Q

K

V

E

G

G

I

V

D

E

V

C

E

E

V

V

I

I

D

N

A

M

Q

R

S

T

L

I

L

R

P

P

F

M

D

D

I

M

N

-

H

E

D

T

V

I

V

E

K

A

S

S

L

V

A

M

K

K

T

T

N

N

R

H

L

L

M

V

I

T

I

V

D

E

E

G

D

G

V

W

P

P

G

Q

-

F

G

G

A

V

S

G

A

A

Y

E

L

I

L

C

D

A

K

R

I

I

L

M

N

-

E

E

Q

G

N

P

G

A

Y

F

Q

N

Y

F

L

L

D

D

S

A

Q

-

P

P

K

A

T

V

L

R

A

V

A

T

K

G

A

A

H

D

R

V

P

P

-

M

A

P

Y

Y

G

A

T

K

D

I

G

L

D

E

Y

D

F

N

S

S

K

I

P

P

S

Q

A

V

E

K

D

D

I

I

Y

I

E

F

A

A

I

I

active site: E61 (= E525), H130 (≠ G594)
binding thiamine diphosphate: I59 (= I523), E61 (= E525), M83 (≠ Q547), F87 (≠ Y551)

Q5SLR4 2-oxoisovalerate dehydrogenase subunit alpha; Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha; EC 1.2.4.4 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
27% identity, 39% coverage: 41:353/803 of query aligns to 59:333/367 of Q5SLR4

query
sites
Q5SLR4

V

I

L

R

T

T

G

G

K

K

A

T

K

S

F
|
F

G

I

I

A

F

P

G

A

D

A

G

G

K

H

E

E

V

A

P

A

Q

Q

L

V

A

A

W

I

A

A

K

H

A

A

F

I

A

R

N

P

G

G

-

F

D

D

F

W

R

V

S

F

G

P

Y
|
Y

R
|
R

D

D

Q

H

T

G

F

L

M

A

M

L

A

A

I

L

G

G

A

-

L

I

D

P

I

L

Q

K

T

E

F

L

S

G

G

Q

L

M

Y

L

A

A

N

T

T

-

D

-

I

-

S

K

L

A

E

D

P

P

M

-

S

N

G

K

G

G

R

R

Q

Q

M
|
M

G
x
P

G
x
E

H
|
H

F

P

S

G

T

S

F

K

S

A

L

L

D

-

E

-

N

-

G

-

Q

-

W

-

N

-

L

-

T

-

K

-

Q

-

K

-

N

N

S

F

S

T

D

V

I

A

S
|
S

P
|
P

T
x
I

A

A

G

S

Q

H

M

V

P

P

R

P

L

A

G

G

L

A

A

A

Q

I

A

S

S

M

K

K

I

L

Y

L

R

R

N

T

V

-

E

-

G

-

I

-

D

-

T

-

K

-

F

-

S

-

N

-

K

-

G

-

N

G

E

Q

I

V

A

A

W

V

G

C

T

T

I

F

G
|
G

N
x
D

A
x
G

S
x
A

T
|
T

S
|
S

E
|
E

G

G

L

D

F

W

F

Y

E

A

T

G

I

I

N

N

A

F

A

A

G

A

V

V

L

Q

Q

G

V

A

P

P

M

A

V

V

I

F

S

I

V

A

W

E

D

N

D
x
N

E
x
F

Y
|
Y

G
x
A

I
|
I

S

S

V

V

H

D

A

Y

R

R

H

H

Q

Q

T

T

T

H

K

S

E

P

N

T

I

I

S

A

E

D

I

K

L

A

S

H

G

A

F

F

Q

-

R

-

D

-

N

-

E

-

A

-

K

-

G

G

Y

I

E

P

I

G

L

Y

R

L

V

V

K

D

G

G

W

M

D

D

Y

V

V

L

A

A

L

S

I

Y

E

Y

T

V

Y

V

Q

K

E

E

A

A

A

V

K

E

I

R

A

A

R

R

T

R

E

G

H

E

V

G

P

P

V

S

L

L

V

V

H

E

V

L

N

R

E

-

L

V

T

Y

Q

R

P

Y

Q

G

G

P

H
|
H

S

S

T

S

S

A

G

D

S

D

H

D

E

S

R

R

Y

Y

K

R

D

P

K

K

E

E

R

E

L

V

A

A

W

F

E

W

A

R

K

K

T

K

D

D

C

P

N

I

V

P

Q

R

L

F

R

R

K

R

W

F

M

L

V

E

E

A

T

R

G

G

I

L

S

W

T

N

D

E

E

E

E

W

L

E

I

E

T

D

I

V

E

R

K

E

G

E

I

I

K

R

K

A

A

E

V

L

R

E

E

R

G

G

K

L

K

K

A

E

A

A

F66 (= F48) binding substrate
YYR 94:96 (= YYR 75:77) binding thiamine diphosphate
Y95 (= Y76) binding substrate
MPEH 128:131 (≠ MGGH 114:117) binding substrate
S144 (= S143) binding substrate
SPI 144:146 (≠ SPT 143:145) binding thiamine diphosphate
174:180 (vs. 186:192, 57% identical) binding thiamine diphosphate
D175 (≠ N187) binding Mg(2+)
N204 (≠ D216) binding Mg(2+)
NFYAI 204:208 (≠ DEYGI 216:220) binding thiamine diphosphate
Y206 (= Y218) binding Mg(2+)
H273 (= H293) binding thiamine diphosphate

1umdA Branched-chain 2-oxo acid dehydrogenase (e1) from thermus thermophilus hb8 with 4-methyl-2-oxopentanoate as an intermediate (see paper)
27% identity, 39% coverage: 41:353/803 of query aligns to 54:328/362 of 1umdA

query
sites
1umdA

V

I

L

R

T

T

G

G

K

K

A

T

K

S

F
|
F

G

I

I

A

F

P

G

A

D

A

G

G

K

H

E

E

V

A

P

A

Q

Q

L

V

A

A

W

I

A

A

K

H

A

A

F

I

A

R

N

P

G

G

-

F

D

D

F

W

R

V

S

F

G

P

Y
|
Y

R
|
R

D

D

Q

H

T

G

F

L

M

A

M

L

A

A

I

L

G

G

A

-

L

I

D

P

I

L

Q

K

T

E

F

L

S

G

G

Q

L

M

Y

L

A

A

N

T

T

-

D

-

I

-

S

K

L

A

E

D

P

P

M

-

S

N

G

K

G

G

R

R

Q

Q

M

M

G

P

G

E

H

H

F

P

S

G

T

S

F

K

S

A

L

L

D

-

E

-

N

-

G

-

Q

-

W

-

N

-

L

-

T

-