SitesBLAST
Comparing WP_055437108.1 NCBI__GCF_001418085.1:WP_055437108.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
38% identity, 94% coverage: 9:419/435 of query aligns to 15:419/425 of O59010
- S65 (≠ A60) mutation to V: Strongly decreased chloride conductance.
- R276 (≠ S281) mutation to S: Increased rate of aspartate transport; when associated with R-395.
- RSS 276:278 (≠ SSS 281:283) binding L-aspartate
- M311 (= M317) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
- T314 (= T320) binding L-aspartate
- V355 (= V361) binding L-aspartate
- D394 (= D394) binding L-aspartate
- M395 (= M395) mutation to R: Increased rate of aspartate transport; when associated with S-276.
- R397 (= R397) mutation to A: Strongly decreased affinity for aspartate.
- N401 (= N401) binding L-aspartate
- D405 (= D405) mutation to N: Strongly decreased affinity for aspartate.
2nwwA Crystal structure of gltph in complex with tboa (see paper)
37% identity, 94% coverage: 9:415/435 of query aligns to 6:406/407 of 2nwwA
6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
37% identity, 94% coverage: 9:415/435 of query aligns to 12:412/413 of 6x14A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G64), V83 (≠ I82), I157 (≠ F171), Y164 (≠ L178), K193 (= K200), T305 (= T314), I306 (= I315), I347 (= I356)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I10), M199 (= M206), S275 (= S283), T311 (= T320), G356 (= G365), L384 (≠ F387), D391 (= D394), R394 (= R397)
Sites not aligning to the query:
6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
37% identity, 94% coverage: 9:415/435 of query aligns to 15:415/419 of 6x15A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (= F41), F46 (= F41), P75 (≠ D70), L91 (≠ M87), F95 (≠ I91), L130 (= L141), I133 (≠ A143), I159 (≠ M170), Y167 (≠ L178), K196 (= K200), G200 (≠ I204), I207 (≠ Y211), F210 (= F214), L250 (≠ I254), I262 (≠ L267), M269 (≠ Q274), T334 (≠ D340), V335 (≠ L341), G336 (≠ S342), T340 (≠ G346), L343 (≠ A349), M399 (≠ V399)
- binding aspartic acid: S277 (= S282), S278 (= S283), T314 (= T320), G354 (≠ A360), A358 (= A364), G359 (= G365), D394 (= D394), R397 (= R397), T398 (= T398)
- binding sodium ion: Y89 (= Y85), T92 (= T88), S93 (≠ T89), G306 (= G312), T308 (= T314), N310 (= N316), N310 (= N316), M311 (= M317), D312 (= D318), S349 (= S355), I350 (= I356), T352 (≠ S358), N401 (= N401), V402 (= V402), D405 (= D405)
Sites not aligning to the query:
6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
37% identity, 94% coverage: 9:415/435 of query aligns to 7:407/408 of 6bauA
- binding cysteine: S270 (= S283), M303 (= M317), T306 (= T320), A345 (= A359), G346 (≠ A360), V347 (= V361), G351 (= G365), D386 (= D394), C389 (≠ R397), T390 (= T398), N393 (= N401)
6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
37% identity, 94% coverage: 9:415/435 of query aligns to 7:407/409 of 6bavA
6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
39% identity, 96% coverage: 8:423/435 of query aligns to 10:421/425 of 6zgbA
5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
39% identity, 96% coverage: 8:423/435 of query aligns to 12:423/427 of 5e9sA
- binding aspartic acid: R274 (≠ S281), S275 (= S282), S276 (= S283), T313 (= T320), G353 (≠ A360), V354 (= V361), A357 (= A364), G358 (= G365), D394 (= D394), R397 (= R397), T398 (= T398)
- binding decyl-beta-d-maltopyranoside: L194 (≠ K200), G198 (≠ I204), Y202 (≠ A208)
- binding sodium ion: Y87 (= Y85), T90 (= T88), S91 (≠ T89), S276 (= S283), G305 (= G312), A306 (= A313), T307 (= T314), N309 (= N316), N309 (= N316), M310 (= M317), D311 (= D318), S348 (= S355), I349 (= I356), G350 (= G357), T351 (≠ S358), N401 (= N401), V402 (= V402), D405 (= D405)
6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
39% identity, 96% coverage: 8:423/435 of query aligns to 9:420/424 of 6zl4A
- binding decyl-beta-d-maltopyranoside: L191 (≠ K200), G195 (≠ I204), R282 (≠ E292)
- binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S281), S272 (= S282), S273 (= S283), M307 (= M317), T310 (= T320), G353 (= G363), A354 (= A364), R394 (= R397), T395 (= T398)
Sites not aligning to the query:
6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
39% identity, 96% coverage: 8:423/435 of query aligns to 12:423/426 of 6xwnB
6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
38% identity, 96% coverage: 8:423/435 of query aligns to 5:412/416 of 6r7rA