SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_055437409.1 NCBI__GCF_001418085.1:WP_055437409.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
54% identity, 91% coverage: 5:228/247 of query aligns to 1:228/232 of 1f3oA

query
sites
1f3oA

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binding adenosine-5'-diphosphate: Y11 (≠ F15), G41 (= G45), G43 (= G47), K44 (= K48), S45 (= S49), T46 (= T50)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
53% identity, 91% coverage: 5:228/247 of query aligns to 1:228/230 of 1l2tA

query
sites
1l2tA

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binding adenosine-5'-triphosphate: Y11 (≠ F15), S40 (= S44), G41 (= G45), S42 (= S46), G43 (= G47), K44 (= K48), S45 (= S49), T46 (= T50), F138 (≠ L140), Q145 (= Q146), S147 (= S148), G149 (= G150), Q150 (= Q151), H204 (= H204)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
51% identity, 91% coverage: 3:227/247 of query aligns to 1:224/226 of 5xu1B

query
sites
5xu1B

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binding magnesium ion: G45 (= G47), K46 (= K48), S47 (= S49)

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
48% identity, 94% coverage: 3:234/247 of query aligns to 2:232/650 of 5ws4A

query
sites
5ws4A

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binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (= S44), G44 (= G45), S48 (= S49)

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
48% identity, 94% coverage: 5:235/247 of query aligns to 3:232/615 of 5lilA

query
sites
5lilA

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A

binding adenosine-5'-triphosphate: F13 (= F15), V22 (≠ A24), S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (= S49), T48 (= T50), Q92 (= Q94), K136 (≠ R139), Q143 (= Q146), S145 (= S148), G147 (= G150), Q148 (= Q151)
binding magnesium ion: S47 (= S49), Q92 (= Q94)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
48% identity, 94% coverage: 5:235/247 of query aligns to 3:232/592 of 5lj7A

query
sites
5lj7A

I

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A

S

T

D

I

L

R

R

N

S

E

Q

K

K

I

F

G

G

F

F

I

I

F

F

Q
|
Q

S

R

Y

Y

N

N

L

L

A

S

R

S

T

L

S

T

A

A

I

A

E

E

N

N

V

V

E

A

L

L

P

P

L

A

L

I

Y

Y

N

-

S

A

K

G

V

M

S

P

T

Q

E

S

E

Q

R

R

R

L

K

E

R

R

A

A

I

K

E

Q

A

L

L

L

K

E

K

K

V

L

G

G

L

L

G

G

E

D

R
x
K

L

W

D

Q

H

N

T

K

P

P

A

N

Q
|
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

S

A

L

L

V

M

N

N

N

G

P

G

V

E

M

I

L

I

L

L

A

A

D

D

E

Q

A

P

T

T

G

G

N

A

L

L

D

D

T

S

R

H

T

S

A

G

Y

E

E

N

I

V

M

M

S

E

I

I

F

L

Q

R

E

Q

L

L

N

H

E

E

Q

E

G

G

I

H

T

T

I

I

A

I

F

M

V

V

T

T

H

H

E

D

E

K

D

H

I

I

A

A

T

A

F

S

S

A

S

N

R

R

T

I

I

I

V

E

L

I

K

K

D

D

G

G

N

E

I

I

Q

S

D

D

N

T

K

Q

N

K

E

R

N

Q

V

V

Q

K

S

S

A

A

binding adenosine-5'-triphosphate: V22 (≠ A24), S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (= S49), T48 (= T50), Q92 (= Q94), K136 (≠ R139), Q143 (= Q146), S145 (= S148), G147 (= G150), Q148 (= Q151)
binding magnesium ion: S47 (= S49), Q92 (= Q94)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
46% identity, 90% coverage: 5:226/247 of query aligns to 4:224/648 of P75831

query
sites
P75831

I

L

K

E

I

L

E

K

D

D

L

I

K

R

R

R

E

S

F

Y

T

P

M

A

G

G

A

D

E

E

T

Q

V

V

R

E

A

V

L

L

R

K

G

G

I

I

S

S

F

L

D

D

I

I

K

Y

E

A

G

G

E

E

F

M

V

V

T

A

I

I

M

V

G

G

S

A

S

S

G

G

S

S

G

G

K
|
K

S

S

T

T

M

L

L

M

N

N

I

I

L

L

G

G

C

C

L

L

D

D

Q

K

P

A

T

T

S

S

G

G

L

T

Y

Y

E

R

I

V

D

A

G

G

V

Q

S

D

V

V

K

A

D

T

L

L

S

D

R

A

N

D

E

A

L

L

A

A

T

Q

I

L

R

R

N

R

E

E

K

H

I

F

G

G

F

F

I

I

F

F

Q

Q

S

R

Y

Y

N

H

L

L

A

S

R

H

T

L

S

T

A

A

I

E

E

Q

N

N

V

V

E

E

L

V

P

P

L

A

L

V

Y

Y

N

-

S

A

K

G

V

L

S

E

T

R

E

K

E

Q

R

R

R

L

K

L

R

R

A

A

I

Q

E

E

A

L

L

L

K

Q

K

R

V

L

G

G

L

L

G

E

E

D

R

R

L

T

D

E

H

Y

T

Y

P

P

A

A

Q

Q

L

L

S

S

G

G

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

S

A

L

L

V

M

N

N

N

G

P

G

V

Q

M

V

L

I

L

L

A

A

D
|
D

E

E

A

P

T

T

G

G

N

A

L

L

D

D

T

S

R

H

T

S

A

G

Y

E

E

E

I

V

M

M

S

A

I

I

F

L

Q

H

E

Q

L

L

N

R

E

D

Q

R

G

G

I

H

T

T

I

V

A

I

F

I

V

V

T

T

H

H

E

D

E

P

D

Q

I

V

A

A

T

A

F

Q

S

A

S

E

R

R

T

V

I

I

V

E

L

I

K

R

D

D

G

G

N

E

I

I

I

V

Q

R

D

N

K47 (= K48) mutation to L: Lack of activity.
D169 (= D171) mutation to N: Lack of activity.

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
45% identity, 90% coverage: 4:226/247 of query aligns to 2:219/223 of 2pclA

query
sites
2pclA

E

E

I

I

I

L

K

R

I

A

E

E

D

N

L

I

K

K

R

K

E

-

F

V

T

I

M

R

G

G

A

Y

E

E

T

I

V

-

R

-

A

-

L

L

R

K

G

G

I

I

S

S

F

L

D

S

I

V

K

K

E

K

G

G

E

E

F

F

V

V

T

S

I

I

M

I

G

G

S

A

S

S

G

G

S

S

G

G

K

K

S

S

T

T

M

L

L

L

N

Y

I

I

L

L

G

G

C

L

L

L

D

D

Q

A

P

P

T

T

S

E

G

G

L

K

Y

V

E

F

I

L

D

E

G

G

V

K

S

E

V

V

K

D

D

Y

L

T

S

N

R

E

N

K

E

E

L

L

A

S

T

L

I

L

R

R

N

N

E

R

K

K

I

L

G

G

F

F

I

V

F

F

Q

Q

S

F

Y

H

N

Y

L

L

L

I

A

P

R

E

T

L

S

T

A

A

I

L

E

E

N

N

V

V

E

I

L

V

P

P

L

M

L

L

Y

K

N

M

S

G

K

K

V

-

S

P

T

K

E

K

E

E

R

A

R

K

K

E

R

R

A

G

I

E

E

Y

A

L

L

L

K

S

K

E

V

L

G

G

L

L

G

G

E

D

R

K

L

L

D

S

H

R

T

K

P

P

A

Y

Q

E

L

L

S

S

G

G

Q

E

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

S

A

L

L

V

A

N

N

N

E

P

P

V

I

M

L

L

L

L

F

A

A

D

D

E
|
E

A
x
P

T
|
T

G

G

N

N

L

L

D

D

T

S

R

A

T

N

A

T

Y

K

E

R

I

V

M

M

S

D

I

I

F

F

Q

L

E

K

L

I

N

N

E

E

Q

G

G

G

I

T

T

S

I

I

A

V

F

M

V

V

T
|
T

H

H

E

E

E

R

D

E

I

L

A

A

T

E

F

L

S

T

S

H

R

R

T

T

I

L

V

E

L

M

K

K

D

D

G

G

N

K

I

V

Q

G

D

E

binding magnesium ion: E165 (= E172), P166 (≠ A173), T167 (= T174), T196 (= T203)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
46% identity, 90% coverage: 1:223/247 of query aligns to 1:223/233 of P75957

query
sites
P75957

M

M

S

N

K

K

E

I

I

L

K

Q

I

C

E

D

D

N

L

L

K

C

R

K

E

R

F

Y

T

Q

M

E

G

G

A

S

E

V

T

Q

V

T

R

D

A

V

L

L

R

H

G

N

I

V

S

S

F

F

D

S

I

V

K

G

E

E

G

G

E

E

F

M

V

M

T

A

I

I

M

V

G
|
G

S

S

G

G

S

S

G

G

K

K

S

S

T

T

M

L

L

L

N

H

I

L

L

L

G

G

C

G

L

L

D

D

Q

T

P

P

T

T

S

S

G

G

L

D

Y

V

E

I

I

F

D

N

G

G

V

Q

S

P

V

M

K

S

D

K

L

L

S

S

R

S

N

A

E

A

L

K

A

A

T

E

I

L

R

R

N

N

E

Q

K

K

I

L

G

G

F

F

I

I

F

Y

Q

Q

S

F

Y

H

N

H

L

L

A

P

R

D

T

F

S

T

A

A

I

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

Y

I

N

G

S

K

K

K

V

-

S

K

T

P

E

A

E

E

R

I

R

N

K

S

R

R

A

A

I

L

E

E

A

M

L

L

K

K

K

A

V

V

G

G

L

L

G

D

E

H

R

R

L

A

D

N

H

H

T

R

P

P

A

S

Q

E

L

L

S

S

G

G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

S

A

L

L

V

V

N

N

P

P

V

R

M

L

L

V

L

L

A

A

D

D

E

E

A

P

T

T

G

G

N

N

L

L

D

D

T

A

R

R

T

N

A

A

Y

D

E

S

I

I

M

F

S

Q

I

L

F

L

Q

G

E

E

L

L

N

N

E

R

-

L

Q

Q

G

G

I

T

T

A

I

F

A

L

F

V

V

V

T

T

H

H

E

D

E

L

D

Q

I

L

A

A

T

K

F

R

S

M

S

S

R

R

T

Q

I

L

V

E

L

M

K

R

D

D

G

G

N

R

I

L

G42 (= G42) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
46% identity, 89% coverage: 5:223/247 of query aligns to 2:220/226 of 7mdyC

query
sites
7mdyC

I

L

K

Q

I

C

E

D

D

N

L

L

K

C

R

K

E

R

F
x
Y

T

Q

M

E

G

G

A

S

E

V

T

Q

V

T

R

D

A

V

L

L

R

H

G

N

I

V

S

S

F

F

D

S

I

V

K

G

E

E

G

G

E

E

F

M

V

M

T

A

I

I

M

V

G

G

S

S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

M

L

L

L

N

H

I

L

L

L

G

G

C

G

L

L

D

D

Q

T

P

P

T

T

S

S

G

G

L

D

Y

V

E

I

I

F

D

N

G

G

V

Q

S

P

V

M

K

S

D

K

L

L

S

S

R

S

N

A

E

A

L

K

A

A

T

E

I

L

R

R

N

N

E

Q

K

K

I

L

G

G

F

F

I

I

F

Y

Q
|
Q

S

F

Y

H

N

H

L

L

A

P

R

D

T

F

S

T

A

A

I

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

Y

I

N

G

S

K

K

K

V

-

S

K

T

P

E

A

E

E

R

I

R

N

K

S

R

R

A

A

I

L

E

E

A

M

L

L

K

K

K

A

V

V

G

G

L

L

G

D

E

H

R

R

L

A

D

N

H
|
H

T

R

P

P

A

S

Q
x
E

L

L

S
|
S

G
|
G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

S

A

L

L

V

V

N

N

P

P

V

R

M

L

L

V

L

L

A

A

D

D

E
|
E

A

P

T

T

G

G

N
|
N

L

L

D

D

T

A

R

R

T

N

A

A

Y

D

E

S

I

I

M

F

S

Q

I

L

F

L

Q

G

E

E

L

L

N

N

E

R

-

L

Q

Q

G

G

I

T

T

A

I

F

A

L

F

V

V

V

T

T

H
|
H

E

D

E

L

D

Q

I

L

A

A

T

K

F

R

S

M

S

S

R

R

T

Q

I

L

V

E

L

M

K

R

D

D

G

G

N

R

I

L

binding adp orthovanadate: Y12 (≠ F15), G42 (= G45), S43 (= S46), G44 (= G47), K45 (= K48), S46 (= S49), T47 (= T50), Q91 (= Q94), H138 (= H142), E142 (≠ Q146), S144 (= S148), G145 (= G149), G146 (= G150), E168 (= E172), N172 (= N176), H201 (= H204)
binding magnesium ion: S46 (= S49), Q91 (= Q94)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
46% identity, 89% coverage: 5:223/247 of query aligns to 2:220/222 of 7arlD

query
sites
7arlD

I

L

K

Q

I

C

E

D

D

N

L

L

K

C

R

K

E

R

F

Y

T

Q

M

E

G

G

A

S

E

V

T

Q

V

T

R

D

A

V

L

L

R

H

G

N

I

V

S

S

F

F

D

S

I

V

K

G

E

E

G

G

E

E

F

M

V

M

T

A

I

I

M

V

G

G

S

S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T

T

M

L

L

L

N

H

I

L

L

L

G

G

C

G

L

L

D

D

Q

T

P

P

T

T

S

S

G

G

L

D

Y

V

E

I

I

F

D

N

G

G

V

Q

S

P

V

M

K

S

D

K

L

L

S

S

R

S

N

A

E

A

L

K

A

A

T

E

I

L

R

R

N

N

E

Q

K

K

I

L

G

G

F

F

I

I

F

Y

Q

Q

S

F

Y

H

N

H

L

L

A

P

R

D

T

F

S

T

A

A

I

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

Y

I

N

G

S

K

K

K

V

-

S

K

T

P

E

A

E

E

R

I

R

N

K

S

R

R

A

A

I

L

E

E

A

M

L

L

K

K

K

A

V

V

G

G

L

L

G

D

E

H

R

R

L

A

D

N

H

H

T

R

P

P

A

S

Q

E

L

L

S

S

G

G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

S

A

L

L

V

V

N

N

P

P

V

R

M

L

L

V

L

L

A

A

D

D

E

E

A

P

T

T

G

G

N

N

L

L

D

D

T

A

R

R

T

N

A

A

Y

D

E

S

I

I

M

F

S

Q

I

L

F

L

Q

G

E

E

L

L

N

N

E

R

-

L

Q

Q

G

G

I

T

T

A

I

F

A

L

F

V

V

V

T

T

H

H

E

D

E

L

D

Q

I

L

A

A

T

K

F

R

S

M

S

S

R

R

T

Q

I

L

V

E

L

M

K

R

D

D

G

G

N

R

I

L

binding adenosine-5'-diphosphate: G42 (= G45), G44 (= G47), K45 (= K48), S46 (= S49)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
45% identity, 90% coverage: 2:223/247 of query aligns to 1:222/229 of 7v8iD

query
sites
7v8iD

S

N

K

K

E

I

I

L

K

Q

I

C

E

D

D

N

L

L

K

C

R

K

E

R

F

Y

T

Q

M

E

G

G

A

S

E

V

T

Q

V

T

R

D

A
x
V

L

L

R

H

G

N

I

V

S

S

F

F

D

S

I

V

K

G

E

E

G

G

E

E

F

M

V

M

T

A

I

I

M

V

G

G

S

S

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

M

L

L

L

N

H

I

L

L

L

G

G

C

G

L

L

D

D

Q

T

P

P

T

T

S

S

G

G

L

D

Y

V

E

I

I

F

D

N

G

G

V

Q

S

P

V

M

K

S

D

K

L

L

S

S

R

S

N

A

E

A

L

K

A

A

T

E

I

L

R

R

N

N

E

Q

K

K

I

L

G

G

F

F

I

I

F

Y

Q
|
Q

S

F

Y

H

N

H

L

L

A

P

R

D

T

F

S

T

A

A

I

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

Y

I

N

G

S

K

K

K

V

-

S

K

T

P

E

A

E

E

R

I

R

N

K

S

R

R

A

A

I

L

E

E

A

M

L

L

K

K

K

A

V

V

G

G

L

L

G

D

E

H

R
|
R

L

A

D

N

H
|
H

T

R

P

P

A

S

Q
x
E

L

L

S
|
S

G

G

G
|
G

Q
x
E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

S

A

L

L

V

V

N

N

P

P

V

R

M

L

L

V

L

L

A

A

D

D

E

Q

A

P

T

T

G

G

N

N

L

L

D

D

T

A

R

R

T

N

A

A

Y

D

E

S

I

I

M

F

S

Q

I

L

F

L

Q

G

E

E

L

L

N

N

E

R

-

L

Q

Q

G

G

I

T

T

A

I

F

A

L

F

V

V

V

T

T

H
|
H

E

D

E

L

D

Q

I

L

A

A

T

K

F

R

S

M

S

S

R

R

T

Q

I

L

V

E

L

M

K

R

D

D

G

G

N

R

I

L

binding phosphoaminophosphonic acid-adenylate ester: V23 (≠ A24), S43 (= S44), G44 (= G45), G46 (= G47), K47 (= K48), S48 (= S49), T49 (= T50), Q93 (= Q94), R137 (= R139), H140 (= H142), E144 (≠ Q146), S146 (= S148), G148 (= G150), E149 (≠ Q151), H203 (= H204)
binding magnesium ion: S48 (= S49), Q93 (= Q94)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
38% identity, 96% coverage: 5:241/247 of query aligns to 1:238/343 of P30750

query
sites
P30750

I

M

I

I

K

K

I

L

E

S

D

N

L

I

K

T

R

K

E

V

F

F

T

H

M

Q

G

G

A

T

E

R

T

T

V

I

R

Q

A

A

L

L

R

N

G

N

I

V

S

S

F

L

D

H

I

V

K

P

E

A

G

G

E

Q

F

I

V

Y

T

G

I

V

M

I

G

G

S

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

M

L

L

I

N

R

I

C

L

V

G

N

C

L

L

L

D

E

Q

R

P

P

T

T

S

E

G

G

L

S

Y

V

E

L

I

V

D

D

G

G

V

Q

S

E

V

L

K

T

D

T

L

L

S

S

R

E

N

S

E

E

L

L

A

T

T

K

I

A

R

R

N

R

E

Q

K

-

I

I

G

G

F

M

I

I

F

F

Q

Q

S

H

Y

F

N

N

L

L

A

S

R

S

T

R

S

T

A

V

I

F

E

G

N

N

V

V

E

A

L

L

P

P

L

L

L

E

Y

L

N

D

S

N

K

-

V

T

S

P

T

K

E

D

E

E

R

V

R

K

K

R

R

R

A

V

I

T

E

E

A

L

L

L

K

S

K

L

V

V

G

G

L

L

G

G

E

D

R

K

L

H

D

D

H

S

T

Y

P

P

A

S

Q

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

S

A

L

L

V

A

N

S

N

N

P

P

V

K

M

V

L

L

A

C

D

D

E
|
E

A

A

T

T

G

S

N

A

L

L

D

D

T

P

R

A

T

T

A

T

Y

R

E

S

I

I

M

L

S

E

I

L

F

L

Q

K

E

D

L

I

N

N

E

R

Q

R

-

L

G

G

I

L

T

T

I

I

A

L

F

L

V

I

T

T

H

H

E

E

E

M

D

D

I

V

A

V

T

K

-

R

F

I

S

C

S

D

R

C

T

V

I

A

V

V

L

I

K

S

D

N

G

G

N

E

I

L

I

I

-

E

Q

Q

D

D

N

T

K

V

N

S

E

E

N

V

V

F

Q

S

S

H

A

P

K

K

V

T

E

P

L

L

A

A

K

Q

40:46 (vs. 44:50, 86% identical) binding ATP
E166 (= E172) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
38% identity, 96% coverage: 4:241/247 of query aligns to 1:239/344 of 3tuzC

query
sites
3tuzC

E

H

I

M

I

I

K

K

I

L

E

S

D

N

L

I

K

T

R

K

E

V

F
|
F

T

H

M
x
Q

G

G

A

T

E

R

T

T

V

I

R

Q

A

A

L

L

R

N

G

N

I

V

S

S

F

L

D

H

I

V

K

P

E

A

G

G

E

Q

F

I

V

Y

T

G

I

V

M

I

G

G

S

A

S

S

G
|
G

S

A

G
|
G

K
|
K

S
|
S

T
|
T

M

L

L

I

N

R

I

C

L

V

G

N

C

L

L

L

D

E

Q

R

P

P

T

T

S

E

G

G

L

S

Y

V

E

L

I

V

D

D

G

G

V

Q

S

E

V

L

K

T

D

T

L

L

S

S

R

E

N

S

E

E

L

L

A

T

T

K

I

A

R

R

N

R

E

Q

K

-

I

I

G

G

F

M

I

I

F

F

Q

Q

S

H

Y

F

N

N

L

L

A

S

R

S

T

R

S

T

A

V

I

F

E

G

N

N

V

V

E

A

L

L

P

P

L

L

L

E

Y

L

N

D

S

N

K

-

V

T

S

P

T

K

E

D

E

E

R

V

R

K

K

R

R

R

A

V

I

T

E

E

A

L

L

L

K

S

K

L

V

V

G

G

L

L

G

G

E

D

R

K

L

H

D

D

H

S

T

Y

P

P

A

S

Q

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

S

A

L

L

V

A

N

S

N

N

P

P

V

K

M

V

L

L

A

C

D

D

E

Q

A

A

T

T

G

S

N

A

L

L

D

D

T

P

R

A

T

T

A

T

Y

R

E

S

I

I

M

L

S

E

I

L

F

L

Q

K

E

D

L

I

N

N

E

R

Q

R

-

L

G

G

I

L

T

T

I

I

A

L

F

L

V

I

T

T

H

H

E

E

E

M

D

D

I

V

A

V

T

K

-

R

F

I

S

C

S

D

R

C

T

V

I

A

V

V

L

I

K

S

D

N

G

G

N

E

I

L

I

I

-

E

Q

Q

D

D

N

T

K

V

N

S

E

E

N

V

V

F

Q

S

S

H

A

P

K

K

V

T

E

P

L

L

A

A

K

Q

binding adenosine-5'-diphosphate: F12 (= F15), Q14 (≠ M17), G42 (= G45), G44 (= G47), K45 (= K48), S46 (= S49), T47 (= T50)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
38% identity, 96% coverage: 4:241/247 of query aligns to 1:239/344 of 3tuiC

query
sites
3tuiC

E

H

I

M

I

I

K

K

I

L

E

S

D

N

L

I

K

T

R

K

E

V

F
|
F

T

H

M

Q

G

G

A

T

E

R

T

T

V

I

R

Q

A

A

L

L

R

N

G

N

I

V

S

S

F

L

D

H

I

V

K

P

E

A

G

G

E

Q

F

I

V

Y

T

G

I

V

M

I

G

G

S

A

S

S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

M

L

L

I

N

R

I

C

L

V

G

N

C

L

L

L

D

E

Q

R

P

P

T

T

S

E

G

G

L

S

Y

V

E

L

I

V

D

D

G

G

V

Q

S

E

V

L

K

T

D

T

L

L

S

S

R

E

N

S

E

E

L

L

A

T

T

K

I

A

R

R

N

R

E

Q

K

-

I

I

G

G

F

M

I

I

F

F

Q

Q

S

H

Y

F

N

N

L

L

A

S

R

S

T

R

S

T

A

V

I

F

E

G

N

N

V

V

E

A

L

L

P

P

L

L

L

E

Y

L

N

D

S

N

K

-

V

T

S

P

T

K

E

D

E

E

R

V

R

K

K

R

R

R

A

V

I

T

E

E

A

L

L

L

K

S

K

L

V

V

G

G

L

L

G

G

E

D

R

K

L

H

D

D

H

S

T

Y

P

P

A

S

Q

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

S

A

L

L

V

A

N

S

N

N

P

P

V

K

M

V

L

L

A

C

D

D

E

Q

A

A

T

T

G

S

N

A

L

L

D

D

T

P

R

A

T

T

A

T

Y

R

E

S

I

I

M

L

S

E

I

L

F

L

Q

K

E

D

L

I

N

N

E

R

Q

R

-

L

G

G

I

L

T

T

I

I

A

L

F

L

V

I

T

T

H

H

E

E

E

M

D

D

I

V

A

V

T

K

-

R

F

I

S

C

S

D

R

C

T

V

I

A

V

V

L

I

K

S

D

N

G

G

N

E

I

L

I

I

-

E

Q

Q

D

D

N

T

K

V

N

S

E

E

N

V

V

F

Q

S

S

H

A

P

K

K

V

T

E

P

L

L

A

A

K

Q

binding adenosine-5'-diphosphate: F12 (= F15), G42 (= G45), A43 (≠ S46), G44 (= G47), K45 (= K48), S46 (= S49), T47 (= T50)

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
38% identity, 96% coverage: 4:241/247 of query aligns to 1:239/344 of 6cvlD

query
sites
6cvlD

E

H

I

M

I

I

K

K

I

L

E

S

D

N

L

I

K

T

R

K

E

V

F
|
F

T

H

M
x
Q

G

G

A

T

E

R

T

T

V
x
I

R

Q

A

A

L

L

R

N

G

N

I

V

S

S

F

L

D

H

I

V

K

P

E

A

G

G

E

Q

F

I

V

Y

T

G

I

V

M

I

G

G

S

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

M

L

L

I

N

R

I

C

L

V

G

N

C

L

L

L

D

E

Q

R

P

P

T

T

S

E

G

G

L

S

Y

V

E

L

I

V

D

D

G

G

V

Q

S

E

V

L

K

T

D

T

L

L

S

S

R

E

N

S

E

E

L

L

A

T

T

K

I

A

R

R

N

R

E

Q

K

-

I

I

G

G

F

M

I

I

F

F

Q

Q

S

H

Y

F

N

N

L

L

A

S

R

S

T

R

S

T

A

V

I

F

E

G

N

N

V

V

E

A

L

L

P

P

L

L

L

E

Y

L

N

D

S

N

K

-

V

T

S

P

T

K

E

D

E

E

R

V

R

K

K

R

R

R

A

V

I

T

E

E

A

L

L

L

K

S

K

L

V

V

G

G

L

L

G

G

E

D

R

K

L

H

D

D

H

S

T

Y

P

P

A

S

Q
x
N

L

L

S
|
S

G

G

Q
|
Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

S

A

L

L

V

A

N

S

N

N

P

P

V

K

M

V

L

L

A

C

D

D

E

Q

A

A

T

T

G

S

N

A

L

L

D

D

T

P

R

A

T

T

A

T

Y

R

E

S

I

I

M

L

S

E

I

L

F

L

Q

K

E

D

L

I

N

N

E

R

Q

R

-

L

G

G

I

L

T

T

I

I

A

L

F

L

V

I

T

T

H
|
H

E

E

E

M

D

D

I

V

A

V

T

K

-

R

F

I

S

C

S

D

R

C

T

V

I

A

V

V

L

I

K

S

D

N

G

G

N

E

I

L

I

I

-

E

Q

Q

D

D

N

T

K

V

N

S

E

E

N

V

V

F

Q

S

S

H

A

P

K

K

V

T

E

P

L

L

A

A

K

Q

binding phosphothiophosphoric acid-adenylate ester: F12 (= F15), Q14 (≠ M17), I19 (≠ V22), S41 (= S44), G42 (= G45), A43 (≠ S46), G44 (= G47), K45 (= K48), S46 (= S49), T47 (= T50), N141 (≠ Q146), S143 (= S148), Q146 (= Q151), H200 (= H204)

8g4cB Bceabs atpgs high res tm (see paper)
40% identity, 81% coverage: 25:223/247 of query aligns to 23:221/248 of 8g4cB

query
sites
8g4cB

L

L

R

K

G

G

I

I

S

D

F

I

D

H

I

I

K

E

E

K

G

G

E

E

F

F

V

V

T

S

I

I

M

M

G

G

S

A

S
|
S

G

G

S
|
S

G

G

K

K

S
x
T

T

T

M

L

L

L

N

N

I

V

L

L

G

S

C

S

L

I

D

D

Q

Q

P

V

T

S

S

H

G

G

L

T

Y

I

E

H

I

I

D

N

G

G

V

N

S

D

V

M

K

T

D

A

L

M

S

K

R

E

N

K

E

Q

L

L

A

A

T

E

I

F

R

R

N

K

E

Q

K

H

I

L

G

G

F

F

I

I

F

F

Q

Q

S

D

Y

Y

N

N

L

L

A

D

R

T

T

L

S

T

A

V

I

K

E

E

N

N

V

I

E

L

L

L

P

P

L

L

L

S

Y

I

N

-

S

T

K

K

V

L

S

S

T

K

E

K

E

E

R

A

R

N

K

R

R

K

A

F

I

E

E

E

A

V

L

A

K

K

K

E

V

L

G

G

L

I

G

Y

E

E

R

L

L

R

D

D

H

K

T

Y

P

P

A

N

Q

E

L

I

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

S

A

L

F

V

I

N

H

N

D

P

P

V

S

M

I

L

I

L

F

A

A

D
|
D

E

E

A

P

T

T

G

G

N

A

L

L

D

D

T

S

R

K

T

S

A

A

Y

S

E

D

I

L

M

L

S

N

I

K

F

L

Q

S

E

Q

L

L

N

N

E

Q

Q

K

-

R

G

N

I

A

T

T

I

I

A

I

F

M

V

V

T

T

H

H

E

D

E

P

D

V

I

A

A

A

T

S

F

Y

S

C

S

G

R

R

T

V

I

I

V

F

L

I

K

K

D

D

G

G

N

Q

I

M

binding phosphothiophosphoric acid-adenylate ester: S42 (= S44), S44 (= S46), T47 (≠ S49), D168 (= D171)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 13

7tchB Bceab e169q variant atp-bound conformation (see paper)
40% identity, 81% coverage: 25:223/247 of query aligns to 22:220/245 of 7tchB

query
sites
7tchB

L

L

R

K

G

G

I

I

S

D

F

I

D

H

I

I

K

E

E

K

G

G

E

E

F

F

V

V

T

S

I

I

M

M

G

G

S

A

S
|
S

G
|
G

S
|
S

G

G

K
|
K

S
x
T

T

T

M

L

L

L

N

N

I

V

L

L

G

S

C

S

L

I

D

D

Q

Q

P

V

T

S

S

H

G

G

L

T

Y

I

E

H

I

I

D

N

G

G

V

N

S

D

V

M

K

T

D

A

L

M

S

K

R

E

N

K

E

Q

L

L

A

A

T

E

I

F

R

R

N

K

E

Q

K

H

I

L

G

G

F

F

I

I

F

F

Q

Q

S

D

Y

Y

N

N

L

L

A

D

R

T

T

L

S

T

A

V

I

K

E

E

N

N

V

I

E

L

L

L

P

P

L

L

L

S

Y

I

N

-

S

T

K

K

V

L

S

S

T

K

E

K

E

E

R

A

R

N

K

R

R

K

A

F

I

E

E

E

A

V

L

A

K

K

K

E

V

L

G

G

L

I

G

Y

E

E

R

L

L

R

D

D

H

K

T

Y

P

P

A

N

Q
x
E

L

I

S

S

G
|
G

Q

Q

Q

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

S

A

L

F

V

I

N

H

N

D

P

P

V

S

M

I

L

I

L

F

A

A

D

D

E

Q

A

P

T

T

G

G

N

A

L

L

D

D

T

S

R

K

T

S

A

A

Y

S

E

D

I

L

M

L

S

N

I

K

F

L

Q

S

E

Q

L

L

N

N

E

Q

Q

K

-

R

G

N

I

A

T

T

I

I

A

I

F

M

V

V

T

T

H

H

E

D

E

P

D

V

I

A

A

A

T

S

F

Y

S

C

S

G

R

R

T

V

I

I

V

F

L

I

K

K

D

D

G

G

N

Q

I

M

binding adenosine-5'-triphosphate: S41 (= S44), G42 (= G45), S43 (= S46), K45 (= K48), T46 (≠ S49), E142 (≠ Q146), G145 (= G149), G146 (= G150)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 19, 21

8w6iD Cryo-em structure of escherichia coli str k12 ftsex complex with atp- gamma-s in peptidisc
38% identity, 89% coverage: 5:223/247 of query aligns to 1:215/219 of 8w6iD

query
sites
8w6iD

I

M

I

I

K

R

I

F

E

E

D

H

L

V

K

S

R

K

E

A

F
x
Y

T

L

M

G

G

G

A

R

E

Q

T

-

V

-

R

-

A

A

L

L

R

Q

G

G

I

V

S

T

F

F

D

H

I

M

K

Q

E

P

G

G

E

E

F

M

V

A

T

F

I

L

M

T

G

G

S

H

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

M

L

L

L

N

K

I

L

L

I

G

C

C

G

L

I

D

E

Q

R

P

P

T

S

S

A

G

G

L

K

Y

I

E

W

I

F

D

S

G

G

V

H

S

D

V

I

K

T

D

R

L

L

S

K

R

N

N

R

E

E

L

V

A

P

T

F

I

L

R

R

N

R

E

Q

K

-

I

I

G

G

F

M

I

I

F

F

Q
|
Q

S

D

Y

H

N

H

L

L

A

M

R

D

T

R

S

T

A

V

I

Y

E

D

N

N

V

V

E

A

L

I

P

P

L

L

L

I

Y

I

N

-

S

A

K

G

V

A

S

S

T

G

E

D

R

I

R

R

K

R

R

R

A

V

I

S

E

A

A

A

L

L

K

D

K

K

V

V

G

G

L

L

G

L

E

D

R
x
K

L

A

D

K

H

N

T

F

P

P

A

I

Q
|
Q

L

L

S
|
S

G
|
G

Q
x
E

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

S

A

L

V

V

V

N

N

N

K

P

P

V

A

M

V

L

L

A

A

D

D

E

E

A

P

T

T

G

G

N

N

L

L

D

D

T

D

R

A

T

L

A

S

Y

E

E

G

I

I

M

L

S

R

I

L

F

F

Q

E

E

E

L

F

N

N

E

R

Q

V

G

G

I

V

T

T

I

V

A

L

F

M

V

A

T

T

H
|
H

E

D

E

I

D

N

-

L

I

I

A

S

T

R

F

R

S

S

S

Y

R

R

T

M

I

L

V

T

L

L

K

S

D

D

G

G

N

H

I

L

binding phosphothiophosphoric acid-adenylate ester: Y11 (≠ F15), S37 (= S44), G38 (= G45), A39 (≠ S46), G40 (= G47), K41 (= K48), S42 (= S49), T43 (= T50), Q86 (= Q94), K130 (≠ R139), Q137 (= Q146), S139 (= S148), G140 (= G149), G141 (= G150), E142 (≠ Q151), H195 (= H204)

P0A9R7 Cell division ATP-binding protein FtsE from Escherichia coli (strain K12) (see paper)
38% identity, 89% coverage: 5:223/247 of query aligns to 1:215/222 of P0A9R7

query
sites
P0A9R7

I

M

I

I

K

R

I

F

E

E

D

H

L

V

K

S

R

K

E

A

F

Y

T

L

M

G

G

G

A

R

E

Q

T

-

V

-

R

-

A

A

L

L

R

Q

G

G

I

V

S

T

F

F

D

H

I

M

K

Q

E

P

G

G

E

E

F

M

V

A

T

F

I

L

M

T

G

G

S

H

S

S

G

G

S

A

G

G

K
|
K

S

S

T

T

M

L

L

L

N

K

I

L

L

I

G
x
C

C

G

L

I

D

E

Q

R

P

P

T

S

S

A

G

G

L

K

Y

I

E

W

I

F

D

S

G

G

V

H

S

D

V

I

K

T

D

R

L

L

S

K

R

N

N

R

E

E

L

V

A

P

T

F

I

L

R

R

N

R

E

Q

K

-

I

I

G

G

F

M

I

I

F

F

Q

Q

S

D

Y

H

N

H

L

L

A

M

R

D

T

R

S

T

A

V

I

Y

E

D

N

N

V

V

E

A

L

I

P

P

L

L

L

I

Y

I

N

-

S

A

K

G

V

A

S

S

T

G

E

D

R

I

R

R

K

R

R

R

A

V

I

S

E

A

A

A

L

L

K

D

K

K

V

V

G

G

L

L

G

L

E

D

R

K

L

A

D

K

H

N

T

F