SitesBLAST
Comparing WP_057507228.1 NCBI__GCF_001431535.1:WP_057507228.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P77165 Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA; EC 1.2.99.6 from Escherichia coli (strain K12) (see paper)
55% identity, 98% coverage: 3:211/213 of query aligns to 20:224/229 of P77165
- C99 (= C86) binding
- C104 (= C91) binding
- G105 (= G92) binding
- C107 (= C94) binding
- C119 (= C106) binding
- C158 (= C145) binding
- C161 (= C148) binding
- C208 (= C195) binding
- C210 (= C197) binding
5g5gA Escherichia coli periplasmic aldehyde oxidase (see paper)
62% identity, 76% coverage: 50:211/213 of query aligns to 12:173/175 of 5g5gA
- binding fe2/s2 (inorganic) cluster: G47 (= G85), C48 (= C86), D49 (= D87), G51 (= G89), C53 (= C91), G54 (= G92), C56 (= C94), C68 (= C106), C107 (= C145), G108 (= G146), C110 (= C148), C157 (= C195), C159 (= C197)
5y6qA Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
57% identity, 77% coverage: 49:211/213 of query aligns to 4:152/157 of 5y6qA
- binding fe2/s2 (inorganic) cluster: G40 (= G85), C41 (= C86), D42 (= D87), G44 (= G89), C46 (= C91), G47 (= G92), C49 (= C94), C61 (= C106), C101 (= C145), G102 (= G146), C104 (= C148), C136 (= C195), C138 (= C197)
- binding pterin cytosine dinucleotide: Q100 (= Q144), C138 (= C197)
4zohC Crystal structure of glyceraldehyde oxidoreductase (see paper)
45% identity, 77% coverage: 50:213/213 of query aligns to 12:159/161 of 4zohC
- binding fe2/s2 (inorganic) cluster: C47 (= C86), S50 (≠ G89), C52 (= C91), G53 (= G92), C55 (= C94), C67 (= C106), C106 (= C145), G107 (= G146), C109 (= C148), C141 (= C195), C143 (= C197)
- binding pterin cytosine dinucleotide: Q105 (= Q144), C143 (= C197)
1t3qA Crystal structure of quinoline 2-oxidoreductase from pseudomonas putida 86 (see paper)
45% identity, 76% coverage: 50:211/213 of query aligns to 6:152/162 of 1t3qA
- binding fe2/s2 (inorganic) cluster: I40 (≠ K84), C42 (= C86), E43 (≠ D87), G45 (= G89), C47 (= C91), G48 (= G92), C50 (= C94), R60 (≠ N104), C62 (= C106), C101 (= C145), G102 (= G146), C104 (= C148), C136 (= C195), C138 (= C197)
- binding pterin cytosine dinucleotide: Q100 (= Q144), C138 (= C197)
1ffuD Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava which lacks the mo-pyranopterin moiety of the molybdenum cofactor (see paper)
41% identity, 77% coverage: 47:211/213 of query aligns to 2:151/156 of 1ffuD
- binding fe2/s2 (inorganic) cluster: C41 (= C86), S44 (≠ G89), H45 (≠ Q90), C46 (= C91), G47 (= G92), C49 (= C94), C61 (= C106), C100 (= C145), G101 (= G146), C103 (= C148), C135 (= C195), C137 (= C197)
1ffvA Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava (see paper)
41% identity, 77% coverage: 47:211/213 of query aligns to 1:150/155 of 1ffvA
- binding fe2/s2 (inorganic) cluster: I38 (≠ K84), C40 (= C86), S43 (≠ G89), C45 (= C91), G46 (= G92), C48 (= C94), C60 (= C106), C99 (= C145), G100 (= G146), C102 (= C148), C134 (= C195), C136 (= C197)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q98 (= Q144), C136 (= C197)
4usaA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with trans-cinnamaldehyde (see paper)
43% identity, 75% coverage: 52:211/213 of query aligns to 6:153/907 of 4usaA
- binding fe2/s2 (inorganic) cluster: V38 (≠ K84), C40 (= C86), E41 (≠ D87), G43 (= G89), C45 (= C91), G46 (= G92), C48 (= C94), R58 (≠ N104), C60 (= C106), C100 (= C145), G101 (= G146), C103 (= C148), C137 (= C195), C139 (= C197)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q144), C139 (= C197)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 531, 532, 535, 539
- binding hydrocinnamic acid: 255, 425, 494, 497, 535, 626
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us9A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with 3- phenylpropionaldehyde (see paper)
43% identity, 75% coverage: 52:211/213 of query aligns to 6:153/907 of 4us9A
- binding fe2/s2 (inorganic) cluster: V38 (≠ K84), C40 (= C86), E41 (≠ D87), G43 (= G89), C45 (= C91), G46 (= G92), C48 (= C94), R58 (≠ N104), C60 (= C106), C100 (= C145), G101 (= G146), C103 (= C148), C137 (= C195), C139 (= C197)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q144), C139 (= C197)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 3-phenylpropanal: 255, 257, 258, 752
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us8A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with benzaldehyde (see paper)
43% identity, 75% coverage: 52:211/213 of query aligns to 6:153/907 of 4us8A
- binding fe2/s2 (inorganic) cluster: V38 (≠ K84), C40 (= C86), E41 (≠ D87), G43 (= G89), C45 (= C91), G46 (= G92), C48 (= C94), R58 (≠ N104), C60 (= C106), C100 (= C145), G101 (= G146), C103 (= C148), C137 (= C195), C139 (= C197)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q144), C139 (= C197)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539
- binding benzaldehyde: 255, 255, 394, 425, 425, 425, 425, 497, 497, 501, 531, 535, 535, 626, 626, 626, 694, 696, 697
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4c7yA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with sodium dithionite and sodium sulfide (see paper)
43% identity, 75% coverage: 52:211/213 of query aligns to 6:153/907 of 4c7yA
- binding fe2/s2 (inorganic) cluster: C40 (= C86), E41 (≠ D87), G43 (= G89), C45 (= C91), G46 (= G92), C48 (= C94), R58 (≠ N104), C60 (= C106), C100 (= C145), G101 (= G146), C103 (= C148), C137 (= C195), C139 (= C197)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q144), C139 (= C197)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 535, 539
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
- binding hydrogen peroxide: 696, 697, 869
3fc4A Ethylene glycol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
43% identity, 75% coverage: 52:211/213 of query aligns to 6:153/907 of 3fc4A
- binding fe2/s2 (inorganic) cluster: V38 (≠ K84), C40 (= C86), E41 (≠ D87), G43 (= G89), C45 (= C91), G46 (= G92), C48 (= C94), R58 (≠ N104), C60 (= C106), C100 (= C145), G101 (= G146), C103 (= C148), C137 (= C195), C139 (= C197)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q144), C139 (= C197)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 1,2-ethanediol: 535, 622, 696, 697, 869
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
3fahA Glycerol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
43% identity, 75% coverage: 52:211/213 of query aligns to 6:153/907 of 3fahA
- binding fe2/s2 (inorganic) cluster: V38 (≠ K84), C40 (= C86), E41 (≠ D87), G43 (= G89), C45 (= C91), G46 (= G92), C48 (= C94), R58 (≠ N104), C60 (= C106), C100 (= C145), G101 (= G146), C103 (= C148), C137 (= C195), C139 (= C197)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q144), C139 (= C197)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding glycerol: 416, 535, 622, 683, 696, 697, 869, 884, 889, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
1sijA Crystal structure of the aldehyde dehydrogenase (a.K.A. Aor or mop) of desulfovibrio gigas covalently bound to [aso3]- (see paper)
43% identity, 75% coverage: 52:211/213 of query aligns to 6:153/907 of 1sijA
- binding fe2/s2 (inorganic) cluster: V38 (≠ K84), C40 (= C86), E41 (≠ D87), G43 (= G89), C45 (= C91), G46 (= G92), C48 (= C94), R58 (≠ N104), C60 (= C106), Q99 (= Q144), C100 (= C145), G101 (= G146), C103 (= C148), C137 (= C195), C139 (= C197)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q144), C139 (= C197)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding arsenite: 535, 696, 697, 869
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 698, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
Q46509 Aldehyde oxidoreductase; Molybdenum iron sulfur protein; EC 1.2.99.7 from Megalodesulfovibrio gigas (Desulfovibrio gigas) (see paper)
43% identity, 75% coverage: 52:211/213 of query aligns to 6:153/907 of Q46509
- C40 (= C86) binding
- C45 (= C91) binding
- C48 (= C94) binding
- C60 (= C106) binding
- C100 (= C145) binding
- C103 (= C148) binding
- C137 (= C195) binding
- C139 (= C197) binding
P19921 Carbon monoxide dehydrogenase small chain; CO dehydrogenase subunit S; CO-DH S; EC 1.2.5.3 from Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) (Oligotropha carboxidovorans) (see 2 papers)
43% identity, 77% coverage: 47:211/213 of query aligns to 3:153/166 of P19921
- C42 (= C86) binding
- C47 (= C91) binding
- C50 (= C94) binding
- C62 (= C106) binding
- C102 (= C145) binding
- C105 (= C148) binding
- C137 (= C195) binding
- C139 (= C197) binding
1n5wA Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
43% identity, 77% coverage: 47:211/213 of query aligns to 1:151/161 of 1n5wA
- binding flavin-adenine dinucleotide: S43 (≠ G89), H44 (≠ Q90)
- binding fe2/s2 (inorganic) cluster: I38 (≠ K84), G39 (= G85), C40 (= C86), S43 (≠ G89), C45 (= C91), G46 (= G92), C48 (= C94), C60 (= C106), C100 (= C145), G101 (= G146), C103 (= C148), C135 (= C195), C137 (= C197)
1n5wD Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
43% identity, 77% coverage: 47:211/213 of query aligns to 1:151/158 of 1n5wD
- binding flavin-adenine dinucleotide: S43 (≠ G89), H44 (≠ Q90)
- binding fe2/s2 (inorganic) cluster: C40 (= C86), S43 (≠ G89), C45 (= C91), G46 (= G92), C48 (= C94), C60 (= C106), C100 (= C145), G101 (= G146), C103 (= C148), C135 (= C195), C137 (= C197)
- binding pterin cytosine dinucleotide: Q99 (= Q144), C137 (= C197)
3hrdD Crystal structure of nicotinate dehydrogenase (see paper)
38% identity, 79% coverage: 45:212/213 of query aligns to 1:153/160 of 3hrdD
- binding flavin-adenine dinucleotide: E44 (≠ Q88), G45 (= G89), E46 (≠ Q90)
- binding fe2/s2 (inorganic) cluster: E40 (≠ K84), C42 (= C86), S43 (≠ D87), G45 (= G89), C47 (= C91), G48 (= G92), C50 (= C94), C62 (= C106), Q100 (= Q144), C101 (= C145), G102 (= G146), C104 (= C148), C136 (= C195), C138 (= C197)
- binding pterin cytosine dinucleotide: Q100 (= Q144), C138 (= C197)
Q0QLF3 Nicotinate dehydrogenase small FeS subunit; NDH; Nicotinic acid hydroxylase small FeS subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
38% identity, 79% coverage: 45:212/213 of query aligns to 1:153/157 of Q0QLF3
- C42 (= C86) binding
- C47 (= C91) binding
- C50 (= C94) binding
- C62 (= C106) binding
- C101 (= C145) binding
- C104 (= C148) binding
- C136 (= C195) binding
- C138 (= C197) binding
Query Sequence
>WP_057507228.1 NCBI__GCF_001431535.1:WP_057507228.1
MTDVRLTRRELLAMGATTTAGAVAVLSPLPARATANEGQSRTSPVNESTVSLEVNGRPVV
LQVDNRSSLLDVLREQLRLTGTKKGCDQGQCGACTILVDGRRINSCLTLAIMFDGASITT
IEGIVTAGQTHPLQRAFVKHDGYQCGYCTPGQICSAIGALDEIERGVPSFASDDPAAMPK
RTEHEIRERMSGNLCRCGAYPNIVAAIIESAES
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory