SitesBLAST
Comparing WP_057507564.1 NCBI__GCF_001431535.1:WP_057507564.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4zz7A Crystal structure of methylmalonate-semialdehyde dehydrogenase (dddc) from oceanimonas doudoroffii (see paper)
48% identity, 95% coverage: 11:484/501 of query aligns to 7:481/489 of 4zz7A
- active site: N149 (= N154), K172 (= K177), L246 (≠ M251), C280 (= C285), E382 (= E385), A462 (≠ P465)
- binding nicotinamide-adenine-dinucleotide: T146 (= T151), P147 (= P152), F148 (= F153), N149 (= N154), K172 (= K177), E175 (= E180), K205 (≠ E210), V208 (= V213), F222 (= F227), V223 (= V228), G224 (= G229), S225 (= S230), I228 (≠ V233), L246 (≠ M251), G247 (≠ M252), C280 (= C285), E382 (= E385), F384 (= F387)
4iymC Crystal structure of putative methylmalonate-semialdehyde dehydrogenase from sinorhizobium meliloti 1021 complexed with NAD, target 011934
48% identity, 95% coverage: 11:484/501 of query aligns to 10:485/491 of 4iymC
- active site: N153 (= N154), K176 (= K177), F250 (≠ M251), C284 (= C285), E386 (= E385), Q466 (≠ P465)
- binding nicotinamide-adenine-dinucleotide: I149 (= I150), T150 (= T151), P151 (= P152), F152 (= F153), N153 (= N154), F154 (= F155), K176 (= K177), K209 (≠ E210), V212 (= V213), F226 (= F227), V227 (= V228), G228 (= G229), S229 (= S230), I232 (≠ V233), G251 (≠ M252), C284 (= C285), E386 (= E385), F388 (= F387)
P42412 Malonate-semialdehyde dehydrogenase; MSA dehydrogenase; Methylmalonate-semialdehyde dehydrogenase; MMSA dehydrogenase; MMSDH; MSDH; EC 1.2.1.27 from Bacillus subtilis (strain 168) (see 3 papers)
47% identity, 95% coverage: 7:484/501 of query aligns to 6:481/487 of P42412
- C36 (≠ A37) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
- R107 (= R108) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- A150 (≠ T151) binding NAD(+)
- F152 (= F153) binding NAD(+)
- C160 (≠ L161) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
- K176 (= K177) binding NAD(+)
- E179 (= E180) binding NAD(+)
- R180 (≠ Q181) binding NAD(+)
- S229 (= S230) binding NAD(+)
- T251 (≠ M252) binding NAD(+)
- R283 (= R284) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- C287 (≠ A288) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
- C351 (≠ L351) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
- E382 (= E385) binding NAD(+)
- C413 (≠ A416) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.
1t90A Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
47% identity, 95% coverage: 7:484/501 of query aligns to 4:479/484 of 1t90A
- active site: N151 (= N154), K174 (= K177), L248 (≠ M251), C282 (= C285), E380 (= E385), A460 (≠ P465)
- binding nicotinamide-adenine-dinucleotide: I147 (= I150), A148 (≠ T151), P149 (= P152), F150 (= F153), N151 (= N154), W159 (= W162), K174 (= K177), E177 (= E180), R178 (≠ Q181), H207 (≠ E210), V225 (= V228), G226 (= G229), S227 (= S230), V230 (= V233), L248 (≠ M251), T249 (≠ M252), C282 (= C285), E380 (= E385), F382 (= F387)
5tjrD X-ray crystal structure of a methylmalonate semialdehyde dehydrogenase from pseudomonas sp. Aac (see paper)
47% identity, 95% coverage: 8:484/501 of query aligns to 3:455/468 of 5tjrD
- active site: N144 (= N154), K167 (= K177), L241 (≠ M251), C270 (= C285), E356 (= E385), A436 (≠ P465)
- binding adenosine-5'-diphosphate: I140 (= I150), T141 (= T151), F143 (= F153), K167 (= K177), E170 (= E180), K200 (≠ E210), F217 (= F227), S220 (= S230), I223 (≠ V233)
5gtlA NADPH complex structure of aldehyde dehydrogenase from bacillus cereus
34% identity, 95% coverage: 7:480/501 of query aligns to 16:486/491 of 5gtlA
- active site: N165 (= N154), K188 (= K177), E263 (≠ M251), C297 (= C285), E394 (= E385), E471 (≠ D462)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I161 (= I150), P163 (= P152), K188 (= K177), A190 (≠ S179), E191 (= E180), Q192 (= Q181), G221 (= G209), G225 (≠ V213), G241 (= G229), S242 (= S230), T245 (≠ V233), L264 (≠ M252), C297 (= C285), E394 (= E385), F396 (= F387)
5gtkA NAD+ complex structure of aldehyde dehydrogenase from bacillus cereus
34% identity, 95% coverage: 7:480/501 of query aligns to 16:486/491 of 5gtkA
- active site: N165 (= N154), K188 (= K177), E263 (≠ M251), C297 (= C285), E394 (= E385), E471 (≠ D462)
- binding nicotinamide-adenine-dinucleotide: I161 (= I150), I162 (≠ T151), P163 (= P152), W164 (≠ F153), K188 (= K177), E191 (= E180), G221 (= G209), G225 (≠ V213), A226 (≠ N214), F239 (= F227), G241 (= G229), S242 (= S230), T245 (≠ V233), Y248 (≠ H236), L264 (≠ M252), C297 (= C285), Q344 (≠ A331), R347 (= R334), E394 (= E385), F396 (= F387)
4neaA 1.90 angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betb) from staphylococcus aureus in complex with NAD+ and bme-free cys289 (see paper)
30% identity, 95% coverage: 9:485/501 of query aligns to 19:503/505 of 4neaA
- active site: N166 (= N154), K189 (= K177), E264 (≠ M251), C298 (= C285), E399 (= E385), E476 (≠ D462)
- binding nicotinamide-adenine-dinucleotide: P164 (= P152), K189 (= K177), E192 (= E180), G222 (= G209), G226 (≠ V213), G242 (= G229), G243 (≠ S230), T246 (≠ V233), H249 (= H236), I250 (≠ V237), C298 (= C285), E399 (= E385), F401 (= F387)
4go4A Crystal structure of pnpe in complex with nicotinamide adenine dinucleotide
34% identity, 94% coverage: 9:480/501 of query aligns to 4:473/487 of 4go4A
- active site: N149 (= N154), K172 (= K177), E247 (≠ M251), C281 (= C285), E381 (= E385), E458 (≠ P465)
- binding nicotinamide-adenine-dinucleotide: I145 (= I150), V146 (≠ T151), W148 (≠ F153), N149 (= N154), F154 (≠ I159), K172 (= K177), G205 (= G209), G209 (≠ V213), Q210 (≠ N214), F223 (= F227), T224 (≠ V228), G225 (= G229), S226 (= S230), T229 (≠ V233), E247 (≠ M251), G249 (= G253), C281 (= C285), E381 (= E385), F383 (= F387)
6wsbA Crystal structure of a betaine aldehyde dehydrogenase from burkholderia pseudomallei bound to cofactor NAD (see paper)
35% identity, 89% coverage: 9:454/501 of query aligns to 7:458/489 of 6wsbA